From daemon Fri Oct 1 01:56:38 1999 Received: by net.bio.net (8.9.1a/8.9.1) id BAA06968; Fri, 1 Oct 1999 01:56:38 -0700 (PDT) Date: Fri, 1 Oct 1999 01:56:38 -0700 (PDT) Message-Id: <199910010856.BAA06968@net.bio.net> To: annelida@net.bio.net From: "Rafael Sarda" Reply-To: annelida@net.bio.net Subject: Re: Capitella Dear Geoff, Tom, and everybody: May be Capitella capitata Cmplx sounds awful systematically speaking, but if you are doing ecological based work it is probably the best practice. It is simply not feasible to classify Capitella species if you are doing this kind of work. My best wishes. Rafael Sarda -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Fri Oct 1 13:52:09 1999 Received: by net.bio.net (8.9.1a/8.9.1) id NAA25795; Fri, 1 Oct 1999 13:52:09 -0700 (PDT) Date: Fri, 1 Oct 1999 13:52:09 -0700 (PDT) Message-Id: <199910012052.NAA25795@net.bio.net> To: annelida@net.bio.net From: "Judith A. Fournier" <110275.1004@compuserve.com> Reply-To: annelida@net.bio.net Subject: Re: Capitella Dear Folks, I agree with Tom and Rafael. In non-systematic ecological studies, trying to determine if you have one "species" or another of Capitella capitata is beyond the scope of the study (and not feasible in normal environmental assessment time frames.) I like the term "Cmplx". We have been using "Capitella capitata-group" with a similar notation. I will probably continue using this one as it seems to have made its way into many species lists from the east coast of Canada. As to whether or not we actually have discrete species or simply environmentally-induced morphological and/or reproductive variations, that will take a LOT of time to sort out and we may never be able to make species determinations with any sort of confidence based on morphological characters. We know that some groups appear to exhibit differences in sperm morphology and there appear to be differences in genetic material, but these procedures are not appropriate to routine identifications. Judy Fournier <110275.1004@compuserve.com> -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Fri Oct 1 15:53:00 1999 Received: by net.bio.net (8.9.1a/8.9.1) id PAA13204; Fri, 1 Oct 1999 15:53:00 -0700 (PDT) Date: Fri, 1 Oct 1999 15:53:00 -0700 (PDT) Message-Id: <199910012253.PAA13204@net.bio.net> To: annelida@net.bio.net From: "Geoff Read" Reply-To: annelida@net.bio.net Organization: Nat Inst Water Atmosp NZ Subject: Re: Capitella > In non-systematic ecological studies, trying > to determine if you have one "species" or another of Capitella capitata is > beyond the scope of the study All I will say at the moment is that the studies I have in mind are not the routine ecological reports that evidently Tom, Judith and Rafael have in mind, but are papers instead that have as main aim to differentiate ad hoc Capitella species in any way, usually without a morphological component, and use the words 'Capitella capitata,' and sibling or cryptic species somewhere in abstract or title. Go look. You'll find them. It's a whole subculture that have 'described' new species in their reports, but with data mostly lacking the detail necessary for that purpose. From what is published I don't get a sense an adequate knowledge is expressed about the background to the taxonomy of C. capitata, or of its known morphology, or how to use binominals. This is simply not what they're interested in. I have difficulty seeing the point of revealing 'entity one' is different from 'entity two' if we don't know how to relate the entities to the rest of the capitellids or to someone else's entities. I suspect they have left few voucher specimens, should anyone wish to review the papers for the purposes of taxonomy or any other comparison. Geoff -- Geoff Read -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Sat Oct 2 13:40:20 1999 Received: by net.bio.net (8.9.1a/8.9.1) id NAA18186; Sat, 2 Oct 1999 13:40:20 -0700 (PDT) Date: Sat, 2 Oct 1999 13:40:20 -0700 (PDT) Message-Id: <199910022040.NAA18186@net.bio.net> To: annelida@net.bio.net From: James Blake Reply-To: annelida@net.bio.net Subject: Re: Capitella Dear Folks, I agree with Geoff in that we need to sort out the Capitella sibling species complex and apply names to go with the entities. In the soon to be published and last annelid volume (7) of the "Taxonomic Atlas of the Santa Maria Basin Fauna....", I have attempted to set the framework for the revision of Capitella that Geoff is talking about. I review the recent taxonomic history including the classification schemes of Hartman and Warren, and where those schemes apply to the sibling species issues arising from the discoveries of Judy Grassle and others. I restrict Capitella capitata sensu lato to specimens having capillaries in both rami of setigers 1-6 and either capillaries, hooks, or mixed fascicles on setiger 7. I present a table of six of the sibling species that Judy and others have studied and point out that four of these belong to this broadly defined definition. However, when one looks at the table, it will be very apparent that each of those four siblings can be readily separated from one another on the basis of prostomial shape, nature of the peristomium, shape of the posterior end, distribution of hooded hooks, and several of the reproductive and developmental features. Among the specimens I examined from California were three different morphotypes that fall into the "sensu lato" restriction I have suggested. At least one of these is clearly sibling species I; I don't know if the other two belong to other defined siblings or are further new species. However, the point is that I am able to distinguish what appear to be different species based on adult morphology alone. One of these three has a distinct methyl green staining pattern, the others do not. What is needed is to acquire a collection of adult Capitella from Naples, the type locality and to establish and fix a definition of C. capitata from there. Once that is done, then the various siblings can be sorted out and named. I have set up a matrix of what I have and run some preliminary cladistics and given the relatively large number of characters available, the resolution among the siblings is very good with at least one good synapomorphy at each branch. Therefore, I am quite confident that the group can be sorted out at some point and some names applied. This really is quite crucial because C. capitata or whatever one calls it these days, is very important in coastal ecology and is sometimes used a test organism. When that is done, it becomes a matter of comparing apples and oranges as far as comparative test results are concerned. I am probably not the one who will ultimately resolve this issue (capitellids are not my favorite worms), but it was interesting to pull this information together. Hope this helps a little. We are hoping to have vol. 7 in print before the end of the year. Brigitte and I are just wrapping up our last chapters. Jim James A. Blake ENSR Marine & Coastal Center 89 Water Street Woods Hole, MA 02543 -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Sun Oct 3 03:00:08 1999 Received: by net.bio.net (8.9.1a/8.9.1) id DAA08620; Sun, 3 Oct 1999 03:00:08 -0700 (PDT) Date: Sun, 3 Oct 1999 03:00:08 -0700 (PDT) Message-Id: <199910031000.DAA08620@net.bio.net> To: annelida@net.bio.net From: James Blake Reply-To: annelida@net.bio.net Subject: Re: Capitella again Hi Folks, Just a note say that C. capitata's type locality is Greenland, not Naples. Sorry for the mental lapse. I am leaving for sea Monday and am a little stressed out. In this context, I suppose there are some good collections of Capitella from various Greenland locations in the ZMUC. Jim -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Sun Oct 3 13:53:26 1999 Received: by net.bio.net (8.9.1a/8.9.1) id NAA21634; Sun, 3 Oct 1999 13:53:26 -0700 (PDT) Date: Sun, 3 Oct 1999 13:53:26 -0700 (PDT) Message-Id: <199910032053.NAA21634@net.bio.net> To: annelida@net.bio.net From: Jack Pearce Reply-To: annelida@net.bio.net Subject: Re: Capitella again Hi all, Following up on Jim's note, in re: to contacting the ZMUC, I would suggest Mary Peterson as a key contact at Ithe Zool. Mus., U. of Kobenhavn, DK. She may be on this group mailing. Cheers, Jack B. Pearce, North American Editor Marine Pollution Bulletin P.S. Given the past use of group Capitella as an indicator, this entire discussion is extremely important, altho seemingly arcane, to the fields of marine pollution monitoring and experimental ecology; I see several manuscripts/month on the use of Capitella. James Blake wrote: > Just a note say that C. capitata's type locality is Greenland, not Naples. Sorry > for the mental lapse. I am leaving for sea Monday and am a little stressed out. > > In this context, I suppose there are some good collections of Capitella from > various Greenland locations in the ZMUC. -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Mon Oct 4 16:19:25 1999 Received: by net.bio.net (8.9.1a/8.9.1) id QAA11199; Mon, 4 Oct 1999 16:19:25 -0700 (PDT) Date: Mon, 4 Oct 1999 16:19:25 -0700 (PDT) Message-Id: <199910042319.QAA11199@net.bio.net> To: annelida@net.bio.net From: Tom Parker Reply-To: annelida@net.bio.net Organization: LACSD Subject: Re: Capitella James Blake wrote: > In the soon to be published and last annelid volume (7) of the "Taxonomic Atlas > of the Santa Maria Basin Fauna....", I have attempted to set the framework for > the revision of Capitella that Geoff is talking about. > Among the specimens I examined from California were three different > morphotypes that fall into the "sensu lato" restriction I have suggested. At > least one of these is clearly sibling species I; I don't know if the other two > belong to other defined siblings or are further new species. However, the > point is that I am able to distinguish what appear to be different species > based on adult morphology alone. One of these three has a distinct > methyl green staining pattern, the others do not. Greetings: It will be an improvement if we can use a morophology table to distinguish different species...or even groupings of species. Off Palos Verdes I have periodically encountered a recruitment of robust Capitella that have discrete tiny dark epidermal pigment speckles distributed either sparsely or densely over the entire animal. I don't recall such a feature being commented upon in the literature before....does anyone else encounter Capitella capitata Cmplx with such epidermal freckles? If methyl green staining can be relied upon for species discrimination, isn't it likely that these dark freckles might also be of similar discriminatory value? I also have collected sexually mature Capitella capitata Cmplx no larger in length and diameter than a Limnodriloides oligochaete...yet appear sexually mature with genital spines. Is this body size/maturity range common in other collections? bye for now, Tom Parker -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Tue Oct 5 02:45:56 1999 Received: by net.bio.net (8.9.1a/8.9.1) id CAA00813; Tue, 5 Oct 1999 02:45:56 -0700 (PDT) Date: Tue, 5 Oct 1999 02:45:56 -0700 (PDT) Message-Id: <199910050945.CAA00813@net.bio.net> To: annelida@net.bio.net From: "Phil Smith" Reply-To: annelida@net.bio.net Subject: Re: Capitella Tom Parker wrote: > I also have collected sexually mature Capitella capitata Cmplx no larger > in length and diameter than a Limnodriloides oligochaete...yet appear > sexually mature with genital spines. Is this body size/maturity range > common in other collections? All the UK estuarine Capitella capitata Cmplx that I have encountered have been of this size, and a large number are sexually mature. I would be interested to know if anyone in the UK or NW Europe is finding large Capitella capitata Cmplx. Regards, Dr Phil Smith, AEC/3E Ltd, The Limes, Creedy Park, Crediton, Devon, EX17 4EB, UK Tel +44 1363 776456 fax 774656 e-mail earth@online.rednet.co.uk Web site http://www.aecuk.com/ -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Tue Oct 5 04:11:43 1999 Received: by net.bio.net (8.9.1a/8.9.1) id EAA11446; Tue, 5 Oct 1999 04:11:43 -0700 (PDT) Date: Tue, 5 Oct 1999 04:11:43 -0700 (PDT) Message-Id: <199910051111.EAA11446@net.bio.net> To: annelida@net.bio.net From: "Geoff Read" Reply-To: annelida@net.bio.net Subject: Complexity and perplexity Folks, I'm perplexed (or should it be prplxd?). Is vowel aversion going to be the new expressive social phenomenon - like shaven heads or tongue studs? I don't think so. I expect most people have no difficulty striking the extra letters on the keyboard, do not think that abbreviated words have extra magic powers, are able to use database fields of 100's of characters, and so have no reason to adopt 'cmplx' for 'complex.' Thus this message to suggest nipping it in the bud is quite redundant. Yrs Gff :^) -- Geoff Read -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Tue Oct 5 14:42:42 1999 Received: by net.bio.net (8.9.1a/8.9.1) id OAA20515; Tue, 5 Oct 1999 14:42:42 -0700 (PDT) Date: Tue, 5 Oct 1999 14:42:42 -0700 (PDT) Message-Id: <199910052142.OAA20515@net.bio.net> To: annelida@net.bio.net From: Tom Parker Reply-To: annelida@net.bio.net Organization: LACSD Subject: Re: Complexity and perplexity Geoff Read wrote: > I'm perplexed (or should it be prplxd?). Is vowel aversion going to be > the new expressive social phenomenon - like shaven heads or tongue studs? > > I don't think so. I expect most people have no difficulty striking the > extra letters on the keyboard, do not think that abbreviated words have > extra magic powers, are able to use database fields of 100's of > characters, and so have no reason to adopt 'cmplx' for 'complex.' Greetings: You make a good point. My use of "cmplx" was just a repeating of the term adopted by the SCAMIT folks under the Conventions section of the SCAMIT List, Edition 3, page 4-5. I don't recall if there was any specific rationale for dropping the vowel. bye for now, Tom Parker -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Tue Oct 5 14:42:43 1999 Received: by net.bio.net (8.9.1a/8.9.1) id OAA20519; Tue, 5 Oct 1999 14:42:43 -0700 (PDT) Date: Tue, 5 Oct 1999 14:42:43 -0700 (PDT) Message-Id: <199910052142.OAA20519@net.bio.net> To: annelida@net.bio.net From: "Peter Olive" Reply-To: annelida@net.bio.net Subject: Phil Smith wrote about Capitella Dear all I have been reading with interest (and some concern) the correspondance re Capitella. Phil Smith wrote about small but sexually mature Capitella cmplx in British waters. Some time ago I began to get involved with this looking at sexual maturity in populations of Capitella in the R Tyne. My research student Son Ling Zhang obtained excellent data showing that in the R Tyne there were at least two reproductive forms of the Capitella complex. A smaller one (length at maturity less than 10mm which had the larger eggs and non- pelagic larvae and a larger one length at maturity about 20 to 50 mm) with smaller eggs, pelagic larvae. I also got good SEMs of the larvae which differed substantially in the length and pattern of ciliation. Son Lin Zhang tried to distinguish them by patterns of chaetal distribution in relation to other size parameters. I was not convinced that it was really possible though I have no doubt about the existence of two reproductive types (=species?) and have not therefore published this data. Unfortunately a little later a description was published in Chinese with a rather horrid name that is a nomen nudem (if that is the correct term). I still have the original data and the SEMs of the larvae. I would be glad to share these with others trying to make sense of breeding populations of Capitella cmplx in the UK. I do not want to get drawn into the naming of species - not my forte. Good luck to all engaged in this Peter Olive -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Fri Oct 8 12:09:04 1999 Received: by net.bio.net (8.9.1a/8.9.1) id MAA16181; Fri, 8 Oct 1999 12:09:04 -0700 (PDT) Date: Fri, 8 Oct 1999 12:09:04 -0700 (PDT) Message-Id: <199910081909.MAA16181@net.bio.net> To: annelida@net.bio.net Reply-To: annelida@net.bio.net From: "Sergio Salazar Vallejo" Subject: Canary Islands: Pilargids Dear colleagues, Langerhans (1881) described several polychaetes from Puerto de la Orotova, Tenerife, Canary Islands. Among them was Ancistrosyllis albini, which after the review by Pettibone (1966) is regarded as the type species of Synelmis Chamberlin 1919. She also regarded this species as a senior synonym to S. simplex Chamberlin, from Rangiroa Island, Paumotu Islands in the French Polynesia. Together with Harlan Dean, we are preparing a note on Synelmis and have already reviewed some specimens from Cape Vert Islands and some other type materials. However, we have been unable to see any S. albini from the Canary Islands. Jorge Nunez, our expert on Canary Islands polychaetes, has been very helpful but he lacks any specimen of this species. I wonder if any reader in this mailing list might have traveled there and have some pilargids that I could see. Thanks a lot for your help. Un abrazo, Sergio La Ciencia del Tercer Mundo no está perdida, sino ignorada. Third World Science is not lost (Sci. Amer. Aug. 1995:76), just ignored. Sergio I. Salazar-Vallejo Depto. Ecología Acuática ECOSUR, Apdo. Postal 424 Chetumal QR 77000 MEXICO salazar@ecosur-qroo.mx Tel. (983) 20115, 21666 Fax (983) 20447 http://www.ecosur.mx -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Sat Oct 9 03:32:34 1999 Received: by net.bio.net (8.9.1a/8.9.1) id DAA02700; Sat, 9 Oct 1999 03:32:34 -0700 (PDT) Date: Sat, 9 Oct 1999 03:32:34 -0700 (PDT) Message-Id: <199910091032.DAA02700@net.bio.net> To: annelida@net.bio.net From: "Dr. P. Y. Qian" Reply-To: annelida@net.bio.net Subject: Postdoc position Advertisement of Postdoctoral Research Associates Applications are now invited for two postdoctoral research associate positions in my laboratory at the Hong Kong University of Science and Technology. Both posts are open immediately. The university requires that the candidates must have their Ph.D. degree by the time of assuming their duty (or at least certain evidence for completion of Ph.D. degree requirement). Position 1: Chemical Ecologist Duties: To study the chemical communication between settling larvae and chemical properties of substratum. The candidate will team up with a postdoc (chemist) and Ph.D. student in my laboratory to study cues that either induce or inhibit larval settlement of marine invertebrates, particularly major fouling organisms in Hong Kong waters. She/he shall have a good background in analytic chemistry, the procedures and facilities for chemical extraction and separation. Some experience on larval biology of marine invertebrates will be helpful but not essential. Position 2: Benthic ecologist (preferably on polychaetes or echinoderms) Duties: To help in managing projects on environmental impact assessment (EIA) on benthic community. She/he shall have a good background in marine benthic invertebrates (preferably with experience in polychaete systematics). Strong background in biostatistics is essential. Since the candidate needs to spend about 20-30% of his/her time on EIA project, he/she shall be an active research on polychaete biology (preferably on either reproductive or larval biology or benthic community ecology). Salary/benefit: The salary for postdoctoral research associate at this university is about HK$25,000 per month (1US$=HK$7.76), depending on the working experience. The successful candidate will also receive medical and dental insurance. Fund will also be available for the postdoc to attend international conference. However, it is stipulated by the government policy that there will not be any moving expenses for the postdoc. The initial offer will be for one year but with second year renewable. Application Procedures Candidates are invited to send their c.v. and two reference letters to me directly either through e-mail or through fax and the position will remain open until being filled (hope not to be later than early spring). ++++++++++++++++++++++++++++++++++++++++++++++++++++ Dr. Pei-Yuan Qian Associate Professor In Biology Hong Kong University of Science and Technology Clear Water Bay, Hong Kong E-mail:boqianpy@ust.hk Fax: 0852-2358-1559 Tel: 0852-2358-7331 +++++++++++++++++++++++++++++++++++++++++++++++++++++ -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Sun Oct 10 19:46:17 1999 Received: by net.bio.net (8.9.1a/8.9.1) id TAA25341; Sun, 10 Oct 1999 19:46:17 -0700 (PDT) Date: Sun, 10 Oct 1999 19:46:17 -0700 (PDT) Message-Id: <199910110246.TAA25341@net.bio.net> To: annelida@net.bio.net From: "Geoff Read" Reply-To: annelida@net.bio.net Subject: Re: Capitella Some more thoughts on Capitella (capitata) species complexes. The latest Capitella sp. I paper to cross my desk is the one by Horng & Taghon (1999) JMBE 242:41-57. They are looking at organic contaminants and the effects of fecal pellet formation thereon. Their Capitella sp. I culture came direct from Judith Grassle. That is probably fine (except for worries about changes due to inbreeding) but it highlights what is going on. It is difficult to experiment on your local Capitella sibling spp because they are not definable - or so people may think. Horng & Taghon talk of "members of the Capitella sibling species complex" which is fine, but the previous sentence begins, "Capitella capitata, originally believed to be a single species, is now known to consist of a complex of species ..." Oops, we just parted company. Paper after paper after paper commits this mistake. In my scheme of things C. capitata will always be a single species. The question is which, if any, of the so far reported 'siblings' is C. capitata. By the way I would rewrite the above along the lines of, "the morphologically similar capitellids, originally believed to be one species, Capitella capitata, are now known to be ..." It's not at all difficult to be more accurate in name usage. What annelids do Capitella species resemble? They resemble oligochaetes. What do oligochaetologists use routinely to distinguish externally similar species? They use internal anatomy. Have any of the students of the group of species similar to Capitella capitata proved the internal morphologies are identical? Not as far as I am aware. Knowlton (1993) talks of sibling species being artifacts of ignorance. Amen to that. Usually they become pseudo-sibling species once more exhaustively examined. Adopting the term 'sibling species' should not be regarded as an end in itself, nor should the term be used as a crutch. It explains nothing. James Blake wrote: > each of those four siblings can be readily separated from > one another on the basis of prostomial shape, nature of the peristomium, > shape of the posterior end, distribution of hooded hooks, and several of > the reproductive and developmental features. To which can be added genital hook morphology, sperm morphology, chromosome number and karyotypes - thanks to work by Judith Grassle and Kevin Eckelbarger. One thing that worries me is the developmental sequence of capillary setae replacing hooks. I find it hard to believe no one found any variation in setigers 1-6 in juveniles at least. I also note in passing that Capitella sp III (C. jonesi), with capillaries always only on the first three setigers, has never been a sibling species, although included in papers as such. It just doesn't fit C. capitata. Wilcox and Nickell recently (1988: Ophelia 49(2):141-145), and others earlier, report poecilogony in Capitella capitata. For W&N it is on the basis of a difference in egg size in the brood tube of one specimen. I don't know what to make of this observation. Does anyone have any suggestions? > What is needed is to acquire a collection of adult Capitella from Naples, > the type locality and to establish and fix a definition of C. capitata from > there. Jim corrected himself to Greenland. Looking at Fabricius one can see the type locality appears to be a place called 'Pullateriak,' which is helpful. However, one can imagine there might be now, if not in Fabricius's day, more than one species of the complex present there. -- Geoff Read -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Sun Oct 10 23:20:43 1999 Received: by net.bio.net (8.9.1a/8.9.1) id XAA21013; Sun, 10 Oct 1999 23:20:43 -0700 (PDT) Date: Sun, 10 Oct 1999 23:20:43 -0700 (PDT) Message-Id: <199910110620.XAA21013@net.bio.net> To: annelida@net.bio.net From: Damion Reply-To: annelida@net.bio.net Subject: Capitella General Report I'm working on a report about Capitella, in particular, Capitella Sp. I that is found in a Mass. estuary. I'm looking for general pictures/diagrams and information on Capitella sp. Any suggestions on sites I can check out? thanks Damion -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Mon Oct 11 11:20:39 1999 Received: by net.bio.net (8.9.1a/8.9.1) id LAA10262; Mon, 11 Oct 1999 11:20:39 -0700 (PDT) Date: Mon, 11 Oct 1999 11:20:39 -0700 (PDT) Message-Id: <199910111820.LAA10262@net.bio.net> To: annelida@net.bio.net Reply-To: annelida@net.bio.net From: "Eugene D. Gallagher" Subject: Re: Capitella General Report We have at least 4 Capitella sibling species in Boston Harbor (Capitella sp. I, Ia, II, & IIIa). These species were identified by Judy Grassle using starch gel electrophoresis of allozymes. The species also differ markedly in reproductive modes. Some features of their biology and abundance is described in Gallagher & Keay (1998), which I've posted as a pdf on my web page http://www.es.umb.edu/edgwebp.htm. While Capitella sp. I is found in both the intertidal and subtidal, we've never found it to be the numerical dominant. Capitella sp. Ia, which can exceed 8 cm's in length as an adult, was dominant in the subtidal, and Capitella sp. IIIa was dominant in the intertidal. At 11:20 PM 10/10/1999 -0700, you wrote: > I'm working on a report about Capitella, in particular, Capitella Sp. I > that is found in a Mass. estuary. I'm looking for general > pictures/diagrams and information on Capitella sp. Any suggestions on > sites I can check out? Eugene D. Gallagher Environmental, Coastal & Ocean Sciences UMASS/Boston 100 Morrissey Blvd. Boston MA 02125-3393 New Email: Eugene.Gallagher@umb.edu (617) 287-7453 (UMASS) (617) 287-7474 (FAX) -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Mon Oct 11 14:21:07 1999 Received: by net.bio.net (8.9.1a/8.9.1) id OAA01231; Mon, 11 Oct 1999 14:21:07 -0700 (PDT) Date: Mon, 11 Oct 1999 14:21:07 -0700 (PDT) Message-Id: <199910112121.OAA01231@net.bio.net> To: annelida@net.bio.net From: "Petersen, Mary Elizabeth (MSX)" Reply-To: annelida@net.bio.net Subject: Capitella capitata's type locality Monday, 11 October 1999 Dear Friends, Sorry to refute Geoff's helpful comment, but "Pullateriak" is not a place but the Greenlandic name of the worm referred to by Fabricius as Lumbricus capitatus. The Greenlanders had names for many of the common polychaetes, and as far as possible, Fabricius included these names in his comments on the species. These Greenlandic names must have been sent by him to Otto Friderich Mueller, as they are also included in his "Zoologiae Danicae Prodromus seu Animalium ..." (1776), in which many of the diagnoses are ones sent to him by Fabricius. Whether "Pullateriak" or Lumbricus capitatus was being applied to one or more species is not yet certain as despite years of trying, I have not yet been able to get material from the area around Frederikshaab/Paamiut, which is where Fabricius worked and which thus must at least be the type area - we have no specific locality. We have much material from Greenland at the Zoological Museum, University of Copenhagen, but the Frederikshaab area is difficult to get to and material from here thus very poorly represented in our collections. I would be surprized if only one species was present, but to date have not been able to find out. Best wishes, Mary Mary E. Petersen Zoological Museum, University of Copenhagen mepetersen@zmuc.ku.dk -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Mon Oct 11 16:12:38 1999 Received: by net.bio.net (8.9.1a/8.9.1) id QAA11866; Mon, 11 Oct 1999 16:12:38 -0700 (PDT) Date: Mon, 11 Oct 1999 16:12:38 -0700 (PDT) Message-Id: <199910112312.QAA11866@net.bio.net> To: annelida@net.bio.net From: "Geoff Read" Reply-To: annelida@net.bio.net Organization: NIWA (Nat. Inst. Water & Atmos. Res. NZ) Subject: Re: Capitella capitata's type locality > Sorry to refute Geoff's helpful comment, but "Pullateriak" is not a place > but the Greenlandic name of the worm referred to by Fabricius as > Lumbricus capitatus. :-) Two hundred years plus later I think we know which we would prefer he had recorded. In defense of my mistaken assumption the word Pullateriak does follow the word GROENL. on a line of their own, & double- spaced from anything else. But I see now that if it was a place name the nereids, for instance, would be mostly recorded from the same locality - Sengiarak. Very suspicious! Thanks Mary. Geoff -- Geoff Read -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Tue Oct 12 14:47:27 1999 Received: (from daemon@localhost) by net.bio.net (8.9.1a/8.9.1) id OAA05288; Tue, 12 Oct 1999 14:47:27 -0700 (PDT) Date: Tue, 12 Oct 1999 14:47:27 -0700 (PDT) Message-Id: <199910122147.OAA05288@net.bio.net> To: annelida@net.bio.net From: BIOSCI Administrator Reply-To: annelida@net.bio.net Subject: BIOSCI/bionet miniFAQ & Fundraiser ============================================== As most of you will realise the content below my insertion is an automatic message. It is sent each month to help you with your subscription to Bionet lists. Do read it occasionally and KEEP at least ONE for future reference please. INSTRUCTIONS only for ANNELIDA ANNELIDA is a moderated list, mainly to stop spam (junk mail). 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ANNELIDA moderator Part of ANNELIDA Charter: For discussion of the scientific study of Phylum Annelida and of other worms with annelid affinities, encompassing the polychaetes, the clitellates (oligochaetes and leeches), the pogonophora, vestimentifera, sipunculans, and echiurids. ============================================== (LAST REVISION: 14-AUG-99) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- All BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. 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Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- the UK-HGMP-Resource Centre (known as hgmp.mrc.ac.uk): ----------------------------------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@hgmp.mrc.ac.uk. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to majordomo@hgmp.mrc.ac.uk. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Please ask for help at biosci@hgmp.mrc.ac.uk if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Wed Oct 13 13:51:15 1999 Received: by net.bio.net (8.9.1a/8.9.1) id NAA00196; Wed, 13 Oct 1999 13:51:15 -0700 (PDT) Date: Wed, 13 Oct 1999 13:51:15 -0700 (PDT) Message-Id: <199910132051.NAA00196@net.bio.net> To: annelida@net.bio.net From: Nicole Dubilier Reply-To: annelida@net.bio.net Organization: mpi-bremen Subject: Anesthetizing tubificids Dear Annelidians, A while ago I asked for suggestions on anesthetizing lumbricids and received many helpful suggestions. I am now back to my pet worms, gutless marine tubificid oligochaetes, and am trying to get them to hold still while I stick a needle through them . I started off with the classical meiofauna anesthetic MgCl and this worked well but unfortunately MgCl inhibits the symbiotic bacteria in these worms. What I need is an anesthetic that will knock out the worms but leave their bacteria active. I have heard that lidocain, the stuff dentists use on Homo sapiens, is supposed to work well on annelids. Has anyone ever worked with this or have any other suggestions? Cheers, Nicole Dubilier Dr. Nicole Dubilier Dept. of Molecular Ecology Max-Planck Institute for Marine Microbiology Celsiusstr. 1, D-28359 Bremen, Germany Tel.: +49 421 2028-932, Fax: +49 421 2028-580 ndubilie@mpi-bremen.de -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Thu Oct 14 22:20:55 1999 Received: by net.bio.net (8.9.1a/8.9.1) id WAA23328; Thu, 14 Oct 1999 22:20:55 -0700 (PDT) Date: Thu, 14 Oct 1999 22:20:55 -0700 (PDT) Message-Id: <199910150520.WAA23328@net.bio.net> To: annelida@net.bio.net From: Benbowme1@aol.com Reply-To: annelida@net.bio.net Subject: Freshwater oligochaeta from Hawaii Dear All, I am interested in finding someone who may have interest in identifying and possibly describing a very large aquatic oligochaete that I repeatedly collect from mountain streams on Maui, Hawaii. I am positive that they are fully aquatic. A couple of days ago I collected close to 20 (many > 12 cm in length) in about a 225cm2 area of sand covered with detritus. They were buried, and when disturbed actively began to burrow back into the sand. They had full guts and were turgid to the touch. When lifted and left in the water column they actively flail about in a trashing manner until they settle on the substrate and then begin to burrow again. no sign of osmotic stress at all. I have collected them in abundance several times before. The interesting thing is that they have a clittelum (or what appears to be one), which I have been told, is not found in any fully aquatic oligochaeta, but more indicative of earthworms (I think the Lumbriculidae or something like that, I am not a expert in annelid taxonomy, obviously) which are all terrestrial. If anyone has any information or is willing to take a look at these creatures please contact me at benbowme1@aol.com. I can collect as many as is needed. Thanks in advance. Eric Benbow Dept. of Biology University of Dayton Dayton, OH 45469-2320 Address in Hawaii: 2747 S. Kihei Rd. F102 Kihei, HI 96753 808-874-3902 -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Fri Oct 15 00:21:11 1999 Received: by net.bio.net (8.9.1a/8.9.1) id AAA06256; Fri, 15 Oct 1999 00:21:11 -0700 (PDT) Date: Fri, 15 Oct 1999 00:21:11 -0700 (PDT) Message-Id: <199910150721.AAA06256@net.bio.net> To: annelida@net.bio.net Reply-To: annelida@net.bio.net From: Prof Barrie Jamieson Subject: Re: Freshwater oligochaeta from Hawaii Dear Eric, All oligochaete families have a clitellum and the Lumbriculidae are aquatic. Nevertheless, your worm, with a clearly visible clitellum is probably a member of the Crassiclitellata (multilayered clitellum). In this group, Megascolecidae of the genus Pheretima sensu lato occur on Hawaii and they are noted for their 'thrashing' locomotion when disturbed. Pheretima and its near relatives (Amynthas etc) are exceptional in having setae BETWEEN the male pores (as part of a ring of setae, the perichaetine condition (only Propheretima in eastern Australia shares that conditon). If you see: a single midventral female pore on segment 14 (13th setigerous segment), clitellum on segs 14-16, and setae between the male pores, on segment 18, it will be a 'pheretimoid' (Megascolecidae). If not, you could send it to me (ethanol preservation only, in case the DNA is interesting!). Yours, Barrie Jamieson At 10:20 PM 10/14/99 -0700, you wrote: >Dear All, > >I am interested in finding someone who may have interest in identifying >and possibly describing a very large aquatic oligochaete that I repeatedly >collect from mountain streams on Maui, Hawaii. The University of Queensland, Zoology Department Barrie Jamieson Professor of Zoology E-Mail : BJamieson@zoology.uq.edu.au -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Fri Oct 15 00:50:48 1999 Received: by net.bio.net (8.9.1a/8.9.1) id AAA09175; Fri, 15 Oct 1999 00:50:48 -0700 (PDT) Date: Fri, 15 Oct 1999 00:50:48 -0700 (PDT) Message-Id: <199910150750.AAA09175@net.bio.net> To: annelida@net.bio.net From: "Geoff Read" Reply-To: annelida@net.bio.net Organization: NIWA (Nat. Inst. Water & Atmos. Res. NZ) Subject: Resurrection Re: Chloraemidae, Pherusidae, Flabelligeridae A quick disintering and hopefully reburial regarding the effect of junior synonymy of a type genus on a family name replaced before 1961 - as was Chloraemidae by Flabelligeridae. Once I wrote: > Flabelligeridae when it replaced Chloraemidae takes the original date of > Chloraemidae. This is laid down in Article 40b(i) which states > "[Flabelligeridae] takes the precedence of the replaced name of which it > is deemed to be the senior synonym." Chloraemidae is 1849, Pherusidae > is 1850. Pherusidae is not older and does not have priority. The previous > 1964 code actually had these words - "[Flabelligeridae] takes the date of > the rejected name ..." There was subsequent argument over that word 'precedence' which was viewed as inferior to using the word 'priority' with its direct link to the once allpowerful but now somewhat less disruptive Principle of Priority. In the new 2000 code article 40 remains, and the word is replaced by 'priority' which I believe confirms my view at the time of the intended power of that clause. Irrespective of that, the new clause 35.5. Precedence for names in use at higher rank also means that Flabelligeridae cannot be removed. No family- group name in prevailing usage can be displaced solely on the grounds another name is older. Phew, aren't we all relieved about that? Have a happy Friday & weekend. -- Geoff Read -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Sun Oct 17 17:55:46 1999 Received: by net.bio.net (8.9.1a/8.9.1) id RAA11288; Sun, 17 Oct 1999 17:55:46 -0700 (PDT) Date: Sun, 17 Oct 1999 17:55:46 -0700 (PDT) Message-Id: <199910180055.RAA11288@net.bio.net> To: annelida@net.bio.net From: "Geoff Read" Reply-To: annelida@net.bio.net Organization: NIWA (Nat. Inst. Water & Atmos. Res. NZ) Subject: Re: Capitella This recent paper might indicate an opportunity to the more technologically skilled of us, if the last sentence in the abstract is any guide. Townson, H., R. E. Harbach, & T. A. Callan. 1999. DNA identification of museum specimens of the Anopheles gambiae complex: an evaluation of PCR as a tool for resolving the formal taxonomy of sibling species complexes.— Systematic Entomology [Syst. Entomol.], 24(1):95-100. Abstract: "Species-specific rDNA primers were tested for their ability to identify museum specimens of the Anopheles gambiae complex ranging in age from 15 to 93 years. Twenty pinned females of known or inferred identity were selected for study from the collection of The Natural History Museum in London, primarily on grounds of their provenance and/or presumed identity. The abdomens were removed and provided 'blind' to the first author for identification. Most of the specimens (17/20) yielded amplifiable DNA and sixteen were identified to species, fifteen unambiguously. The PCR identifications of twelve specimens matched identifications on museum labels, including seven of unambiguous identity. One of the specimens identified correctly was a 93-year-old syntype of An. gambiae. This study demonstrates that PCR-based methods, by establishing the genetic identity of name-bearing type specimens in museum collections, may help in the application of available names to members of sibling species complexes." -- Geoff Read -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Mon Oct 18 01:49:13 1999 Received: by net.bio.net (8.9.1a/8.9.1) id BAA05705; Mon, 18 Oct 1999 01:49:13 -0700 (PDT) Date: Mon, 18 Oct 1999 01:49:13 -0700 (PDT) Message-Id: <199910180849.BAA05705@net.bio.net> To: annelida@net.bio.net From: "Geoff Read" Reply-To: annelida@net.bio.net Subject: Update to PRO researcher addresses Annelida colleagues, I have updated the online PRO polychaete researcher list. If you have recently asked for a change you may wish to check your entry. The downloadable text file is yet to be updated. http://biodiversity.uno.edu/~worms/pro.html The set of files is created automatically, and I don't check them apart from sampling a few. It's up to individuals to advise me of errors and any oddities they may find. Thanks & cheers, Geoff -- Geoff Read -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Tue Oct 19 14:26:20 1999 Received: by net.bio.net (8.9.1a/8.9.1) id OAA10803; Tue, 19 Oct 1999 14:26:20 -0700 (PDT) Date: Tue, 19 Oct 1999 14:26:20 -0700 (PDT) Message-Id: <199910192126.OAA10803@net.bio.net> To: annelida@net.bio.net From: "Geoff Read" Reply-To: annelida@net.bio.net Organization: NIWA (Nat. Inst. Water & Atmos. Res. NZ) Subject: Re: Capitella > Townson, H., R. E. Harbach, & T. A. Callan. 1999. DNA identification of > museum specimens of the Anopheles gambiae complex: an evaluation of > PCR as a tool for resolving the formal taxonomy of sibling species > complexes.— Systematic Entomology [Syst. Entomol.], 24(1):95-100. A correspondent writes: "The main problem with using PCR determinations on most polychaete material is that at least for recent such, it usually has been fixed in formaldehyde ... ." OK, then how about this .... Technology marches on, I guess. France, S. C., & T. D. Kocher. 1996. DNA sequencing of formalin-fixed crustaceans from archival research collections.— Molecular Marine Biology and Biotechnology, 5(4):304-313. Abstract: "Marine invertebrate collections have historically been maintained in ethanol following fixation in formalin. These collections may represent rare or extinct species or populations, provide detailed time-series samples, or come from presently inaccessible or difficult-to-sample localities. We have tested the viability of obtaining DNA sequence data from formalin- fixed, ethanol-preserved (FFEP) deep-sea crustaceans, and found that nucleotide sequences for mitochondrial 16S rRNA and COI genes can be recovered from FFEP collections of varying age, and that these sequences are unmodified compared to those derived from frozen specimens. These results were repeatable among multiple specimens and collections for several species. Our results indicate that in the absence of fresh or frozen tissues, archived, FFEP specimens may prove a useful source of material for analysis of gene sequence data by PCR and direct sequencing." -- Geoff Read -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Wed Oct 20 03:30:40 1999 Received: by net.bio.net (8.9.1a/8.9.1) id DAA13838; Wed, 20 Oct 1999 03:30:40 -0700 (PDT) Date: Wed, 20 Oct 1999 03:30:40 -0700 (PDT) Message-Id: <199910201030.DAA13838@net.bio.net> To: annelida@net.bio.net Reply-To: annelida@net.bio.net From: Torin Morgan Subject: DNA work on formalin fixed material Dear all, France & Kocher (1996) worked on tissue that was formalin fixed but then stored in ethanol. Note that ethanol is a fixative used for DNA work and may have stabilised the DNA to some extent. The problem comes with material stored long-term in formalin (eg most polychaetes), but take heart from the work done recently by Savioz et al. (1997) in Acta Neuropathologica 93, 408-413: A method for the extraction of genomic DNA from human brain tissue fixed and stored in formalin for many years. It may be interesting to watch developments being made by pathologists looking into the genetic basis of diseases like cancer. They have recently been characterising very useful DNA markers like microsatellites from biopsied tissue that had been originally taken for histochemical analysis. Such tissue would have been formalin-fixed and then embedded in paraffin wax. A quick search through your favourite bibliographic database for "microsatellite" + "formalin" should yield the relevant articles for anyone interested. all the best, Torin Geoff wrote: >A correspondent writes: "The main problem with using PCR determinations >on most polychaete material is that at least for recent such, it usually >has been fixed in formaldehyde ... ." > >OK, then how about this .... Technology marches on, I guess. > >France, S. C., & T. D. Kocher. 1996. DNA sequencing of formalin-fixed >crustaceans from archival research collections.— Molecular Marine >Biology and Biotechnology, 5(4):304-313. ---------------------- Dr Torin Morgan School of Ocean and Earth Sciences Southampton University Southampton Oceanography Centre European Way Southampton SO14 3ZH UK office tel: +44 (0)1703 596270 lab tel: +44 (0)1703 596502 mobile tel: 0976 924159 email: torin@soton.ac.uk http://www.soton.ac.uk/~merg -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Wed Oct 20 15:31:35 1999 Received: by net.bio.net (8.9.1a/8.9.1) id PAA18325; Wed, 20 Oct 1999 15:31:35 -0700 (PDT) Date: Wed, 20 Oct 1999 15:31:35 -0700 (PDT) Message-Id: <199910202231.PAA18325@net.bio.net> To: annelida@net.bio.net From: "Aparna Palmer" Reply-To: annelida@net.bio.net Subject: DNA from Formalin fixed polychaete specimens Dear All: Trying to get amplifiable DNA from formalin-fixed polychaete specimens was an issue near and dear to my heart during my Ph.D. I did attempt to use the France and Kocher (1996) protocol as well as a number of other protocols to get the DNA out and was always unsuccessful with the minute polychaete specimens that I had (syllids). So, I went ahead and designed my own protocol--it is very simple, inexpensive and relies upon the use of CTAB and a bit of PCR parameter manipulation. I am in the process of preparing the protocol for publication, but if anyone wants to give it a try, please contact me and I will be happy to send it to you! I was able to extract high quality DNA and was able to get amplification (via PCR) of up to 500 nucleotides reliably. I can be contacted at: aparna@mesastate.edu Alternatively, I can be reached at the address and phone number below. Cheers! Aparna Palmer Assistant Professor Biological Sciences Mesa State College P.O. Box 2647 Grand Junction, CO 81502 Phone: 970-248-1984 -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Thu Oct 21 02:48:31 1999 Received: by net.bio.net (8.9.1a/8.9.1) id CAA27811; Thu, 21 Oct 1999 02:48:31 -0700 (PDT) Date: Thu, 21 Oct 1999 02:48:31 -0700 (PDT) Message-Id: <199910210948.CAA27811@net.bio.net> To: annelida@net.bio.net Reply-To: annelida@net.bio.net From: Pierre CHEVALDONNE Subject: Re: DNA from Formalin fixed polychaete specimens Dear All, I want to comment briefly on the DNA from FFEP specimens issue. I've had the same kind of experience as Aparna Palmer mentioned. Do not have too much confidence in the technology. Yes, many protocols have been published, and yes, it is a fact that FFEP (Formalin Fixed Ethanol Preserved) specimens can yield good quality DNA. But you will definitely endure a lot of frustration before you can actually obtain some data. I did try to get DNA from large FFEP polychaetes and crabs for about three months using published protocols; without success. Finally it did work for ONE shrimp sample, but I gave up on the worms. So, I am glad there is a new protocol available, but I still think it is a very tricky business. Aparna, please send me your protocol, I'll be glad to give it a try one of these days. Thank you. Pierre Dr. Pierre Chevaldonne Cave & Deep-Sea Biology Group CNRS, Station Marine d'Endoume Centre d'Oceanologie de Marseille Rue de la Batterie des Lions 13007 Marseille, France Tel: 33 4 91 04 16 59 Fax: 33 4 91 04 16 35 E-mail: chevaldonne@com.univ-mrs.fr Web: http://www.com.univ-mrs.fr -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Thu Oct 21 13:46:08 1999 Received: by net.bio.net (8.9.1a/8.9.1) id NAA26019; Thu, 21 Oct 1999 13:46:08 -0700 (PDT) Date: Thu, 21 Oct 1999 13:46:08 -0700 (PDT) Message-Id: <199910212046.NAA26019@net.bio.net> To: annelida@net.bio.net From: beavis@maths.tcd.ie Reply-To: annelida@net.bio.net Subject: Spirorbis spirorbis Greetings Polychaeters! If thats not an indication that I'm a newbie then I dont know what would be. Anyway, down to business. I'm doing a project on Spirorbis spp. and was just wondering if anyone knew of any decent web pages on the subject with good pictures etc. Much appreciated. Ronan Donohoe beavis@maths.tcd.ie Zoology, Trinity College Dublin -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Thu Oct 21 13:51:28 1999 Received: by net.bio.net (8.9.1a/8.9.1) id NAA26891; Thu, 21 Oct 1999 13:51:28 -0700 (PDT) Date: Thu, 21 Oct 1999 13:51:28 -0700 (PDT) Message-Id: <199910212051.NAA26891@net.bio.net> To: annelida@net.bio.net From: msatterw@lcsc.edu Reply-To: annelida@net.bio.net Subject: Re: DNA from Formalin fixed polychaete specimens My two cents worth.... Speaking as a chemist, Formalin is highly reactive and probably derivatizes the DNA. The extent of the derivatization probably depends on the length of exposure, temperature in storage, the concentration of formalin and other factors such as stabilizers, etc. It doesn't surprise me that the results are variable, since samples vary in size, permeability, thickness, etc. If possible you might try sampling tissue from a more isolated section of the preserved specimen. On 21 Oct 99, at 2:48, Pierre CHEVALDONNE wrote: > I want to comment briefly on the DNA from FFEP specimens issue. I've > had the same kind of experience as Aparna Palmer mentioned. Do not > have too much confidence in the technology. msatterw@lcsc.edu -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Fri Oct 22 14:11:41 1999 Received: by net.bio.net (8.9.1a/8.9.1) id OAA17929; Fri, 22 Oct 1999 14:11:41 -0700 (PDT) Date: Fri, 22 Oct 1999 14:11:41 -0700 (PDT) Message-Id: <199910222111.OAA17929@net.bio.net> To: annelida@net.bio.net Reply-To: annelida@net.bio.net From: Elin Sigvaldadottir Subject: Urgent - Polychaete Conference registration reminder Dear colleagues and friends, This is to remind you that the closing date for registration to the 7th International Polychaete Conference is in a few days on November 1st 1999. I am sure there are many of you out there who are going to attend but have not registered yet. Further information about the Conference will only be send to those who have registered - but at this time registration is not binding so do not hesitate to register even if you are not sure of coming to Reykjavik in 2001. You can register by: E-mail - elins@ni.is Web - The conference homepage http://www.ni.is/7IPCI Post - The registration sheet on the Conference-brochure. (If you do not have it in your possession please contact me). With best regards, Elin Elin Sigvaldadottir Icelandic Institute of Natural History Box 5320 125 Reykjavik Iceland Phone: +354-5629822, +354-5626611-209 FAX: +354-5620815 E-mail: elins@ni.is http://www.ni.is/7IPCI -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Fri Oct 22 14:11:41 1999 Received: by net.bio.net (8.9.1a/8.9.1) id OAA17928; Fri, 22 Oct 1999 14:11:41 -0700 (PDT) Date: Fri, 22 Oct 1999 14:11:41 -0700 (PDT) Message-Id: <199910222111.OAA17928@net.bio.net> To: annelida@net.bio.net From: Andrew.Mackie@nmgw.ac.uk Reply-To: annelida@net.bio.net Subject: Museum of Wales website The National Museum of Wales website has just been updated and improved. See: http://websrv1.nmgw.ac.uk/biosyb/index.en.shtml Specimen info. on http://zoology.nmgw.ac.uk:591/Museum/Home.htm Regards, Andy Dr Andrew S.Y. Mackie Head of Marine Biodiversity Section Department of Biodiversity and Systematic Biology (BioSyB) National Museum of Wales Cathays Park Cardiff CF10 3NP UK Tel: int +44 (0)1222 573 311 Fax: int +44 (0)1222 239 009 E-mail: Andrew.Mackie@nmgw.ac.uk Web: http://websrv1.nmgw.ac.uk/biosyb/index.en.shtml Specimens: http://zoology.nmgw.ac.uk:591/Museum/Home.htm -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Wed Oct 27 19:40:57 1999 Received: by net.bio.net (8.9.1a/8.9.1) id NAA10041; Wed, 27 Oct 1999 13:59:49 -0700 (PDT) Date: Wed, 27 Oct 1999 13:59:49 -0700 (PDT) Message-Id: <199910272059.NAA10041@net.bio.net> To: annelida@net.bio.net From: Cutlereb@aol.com Reply-To: annelida@net.bio.net Subject: Echiura Dear Colleagues - Here's a shared point of view about: Echiura: Phylum, Family(ies) or ?? We three, who have some 'hands-on' experience with Echiura would like to share our perspective, in this informal context, on the issue of 'proper' taxonomic rank for this group of worms. Our intent is to open the idea that either to maintain it as a phylum MAY be wrong, but to consider it as a single family COULD BE equally wrong. The problem may concern the separate issues of "clustering" and "ranking". If the echiuran worms are proved non-monophyletic, we might well claim two or more families for this animal group. On the other hand, IF the worms are proved to constitute a monophyletic group, its ranking may be dependent on the reconstructed tree; the family rank might be also possible in the system of Annelida. It is our judgement that to reduce it to a single Family based on the currently available information, is premature. At the very least, if one is going to base this judgement on molecular data, we must look at a representative of the bonellids, a major sub-set, and one with distinctive sexual dimorphism. It is in some ways unfortunate that the morphologically unique genus Urechis (4 putative species each in its own corner of the Pacific) is so easy to obtain because it is the only genus in its Order & Family - a derived taxon whose molecular (and developmental?) attributes may not be an accurate representation of the larger group. Thus, in our minds the questions of number of higher taxa as well as rank remain open. It is our opinion that a richer, more convincing database is needed to support clustering these within the Annelida, and to more firmly establish the number and rank of such group(s). We adopt this more conservative position partly to avoid serial changes in the system that we know are not well appreciated by our non-taxonomist colleagues. Regards, T. Nishikawa, J. I. Saiz-Salinas, E. Cutler ----------------------------------------------------------------------- Teruaki Nishikawa, D. Sc. Graduate School of Human Informatics, Nagoya University, Chikusa-ku, Nagoya 464-8601, JAPAN E-mail: teruaki@info.human.nagoya-u.ac.jp Dr. José I. SAIZ SALINAS Dpto. de Zoología y DCA Univ. País Vasco E-48080 Bilbao, Apdo. 644 SPAIN E-mail: zopsasaj@lg.ehu.es http://www.fauna-iberica.mncn.csic.es/faunai/cvi/salinasi.html Edward B. Cutler, PhD Department of Invertebrate Zoology, Museum of Comparative Zoology Harvard University, Cambridge, MA 02138, USA e-mail: CutlerEB@aol.com http://www.mcz.harvard.edu/Departments/InvertZoo/as.fldr/cutler/cutler.htm -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Thu Oct 28 14:20:33 1999 Received: by net.bio.net (8.9.1a/8.9.1) id OAA23439; Thu, 28 Oct 1999 14:20:33 -0700 (PDT) Date: Thu, 28 Oct 1999 14:20:33 -0700 (PDT) Message-Id: <199910282120.OAA23439@net.bio.net> To: annelida@net.bio.net From: "Kristian Fauchald" Reply-To: annelida@net.bio.net Subject: Re: Echiura The Echiura is such a nice compact group so I do not believe they would ever become scattered all over the Annelida. Thus, if the group is monophyletic, it is going to stay together no matter what; if it consists of two recognizable subunits, fine, if it does not, equally fine. It is actually trivial whether the group is considered a phylum or a family: Ranks such as these are artificial and represent a major problem if one starts to take them seriously. The important point is that the group is clearly monophyletic: There is a branch in any cladogram including these taxa that can be named Echiura, with all sorts of synapomorphies tied to it. I have no clear idea of what its sister-taxon would be. I hope that will become clear as we study them, and the rest of the annelids. I believe it is important that we understand the artificial nature of the ranks: Taking them seriously leads to attempts at comparing them across the animal kingdom, and to all sorts of absurdities, such as naming certain features as family-level characters, genus-level characters etc. The three gentlemen are eminent scholars on their groups, and I believe they should be happy the groups they study are so obviously monophyletic: For us working in a far messier world, this is one of the first goals to reach. Kristian Fauchald -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Thu Oct 28 14:24:32 1999 Received: by net.bio.net (8.9.1a/8.9.1) id OAA24088; Thu, 28 Oct 1999 14:24:32 -0700 (PDT) Date: Thu, 28 Oct 1999 14:24:32 -0700 (PDT) Message-Id: <199910282124.OAA24088@net.bio.net> To: annelida@net.bio.net From: Damhnait McHugh Reply-To: annelida@net.bio.net Subject: Research Assistantship - Molecular Systematics of Annelids SUMMARY: A Research Assistant is sought to participate in an NSF- funded research project on the phylogenetic relationships of a group of marine annelids and the evolution of their larval developmental modes. The Research Assistant will help in collecting nuclear and mitochondrial DNA sequence data for phylogenetic analysis; the results will allow the pattern of change in larval development throughout the evolutionary history of the group to be inferred, and will provide the basis for a re-classification of the group. POSITION: Research Assistant Molecular systematics and phylogeny of annelids NSF-funded salary ($24,000 plus benefits) Full-time, one to two years Department of Biology, Colgate University, Hamilton, NY 13346 STARTING DATE: January, 2000 ESSENTIAL FUNCTIONS: Responsible for completion RNA preparations, cDNA sysnthesis, PCR amplifications, automated sequencing, data management and analysis. Other duties will include monitoring lab equipment and supplies, and general lab management. May help to train students and visitors in DNA sequencing techniques, and may oversee their work. Related duties as required. QUALIFICATIONS: B.Sc. or M.Sc. in biology, with previous experience in molecular techniques, including DNA and RNA isolation, PCR and RT- PCR, automated DNA sequencing and cloning. Ability to lift heavy objects and work with some hazardous materials (phenol, chloroform, formaldehyde). Familiarity with sequencing software and phylogenetic analysis preferred. TO APPLY: Send letter of interest, resume (including curriculum vitae and names of three references) to: Damhnait McHugh, Biology Department, Colgate University, Hamilton, NY 13346, U.S.A. (dmchugh@mail.colgate.edu; FAX: 315-228-7997) ******************* Damhnait McHugh Department of Biology Colgate University Hamilton, NY 13346, U.S.A. Tel: (315) 228-7339 Fax: (315) 228-7997 dmchugh@mail.colgate.edu -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Thu Oct 28 14:32:03 1999 Received: by net.bio.net (8.9.1a/8.9.1) id OAA25404; Thu, 28 Oct 1999 14:32:03 -0700 (PDT) Date: Thu, 28 Oct 1999 14:32:03 -0700 (PDT) Message-Id: <199910282132.OAA25404@net.bio.net> To: annelida@net.bio.net From: "Marie Pendle EG CEFAS" Reply-To: annelida@net.bio.net Subject: Methyl blue/ Methyl green Hello everyone, Following a conversation with a colleague today, I was wondering how many others use the above mentioned stains as an aid to polychaete identification? We use them to accentuate chaetal lobes (Nephtys, Glycera etc.), but I vaguely recall hearing that some animals produce characteristic patterns when these stains are used - does anyone know of any literature published, or is this one of those tools that gets used in- house only? I would be pleased to get any information Many thanks Marie Marie Pendle CEFAS Burnham Laboratory Remembrance Avenue Burnham-on-Crouch Essex. CM0 8HA 01621 787200 ext. 243/246/204 -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Thu Oct 28 15:18:58 1999 Received: by net.bio.net (8.9.1a/8.9.1) id PAA01899; Thu, 28 Oct 1999 15:18:58 -0700 (PDT) Date: Thu, 28 Oct 1999 15:18:58 -0700 (PDT) Message-Id: <199910282218.PAA01899@net.bio.net> To: annelida@net.bio.net Reply-To: annelida@net.bio.net From: MMM Secretariat Subject: Mombasa Mangrove Symposium Subject: Mombasa Mangrove Symposium Dear Colleagues, A meeting will be held in Mombasa, 7-11 September 2000, on Mangrove Macrobenthos and Macrofauna. Any paper dealing directly or indirectly with mangrove fish, birds, mammals, crustaceans, insects, mollusks, annelids and other invertebrates is more than welcome. Further information and a pre-registration form is available at: http://www.specola.unifi.it/MMM/ Hoping to hear from you soon, I remain Sincerely yours, Marco Vannini ********************************************************* International Meeting on Mangrove Macrobenthos (MMM) Prof. Marco Vannini director of the Museum of Zoology "La Specola" of the University of Florence via Romana 17 - 50125 Firenze Italy tel. : +39 55 2288251/9 fax : +39 55 225325 e-mail: mmm@www.specola.unifi.it http://www.unifi.it/unifi/msn/ -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Thu Oct 28 16:29:36 1999 Received: by net.bio.net (8.9.1a/8.9.1) id QAA10390; Thu, 28 Oct 1999 16:29:36 -0700 (PDT) Date: Thu, 28 Oct 1999 16:29:36 -0700 (PDT) Message-Id: <199910282329.QAA10390@net.bio.net> To: annelida@net.bio.net From: Tom Parker Reply-To: annelida@net.bio.net Organization: LACSD Subject: Re: Methyl blue/ Methyl green Marie Pendle EG CEFAS wrote: > any literature published, or is this one of those tools that gets used in- > house only? I would be pleased to get any information Dear Marie: I would suggest you inspect the SCAMIT web site at . They have discussed and utilized these stains for polychaete identification (formulas, uses, stain patterns,etc). Much of this information is contained in the newsletters -- some of which are linked to the web site home page. bye for now Tom Parker -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Thu Oct 28 18:22:52 1999 Received: by net.bio.net (8.9.1a/8.9.1) id SAA23224; Thu, 28 Oct 1999 18:22:52 -0700 (PDT) Date: Thu, 28 Oct 1999 18:22:52 -0700 (PDT) Message-Id: <199910290122.SAA23224@net.bio.net> To: annelida@net.bio.net From: "Geoff Read" Reply-To: annelida@net.bio.net Organization: NIWA (Nat. Inst. Water & Atmos. Res. NZ) Subject: Re: Methyl blue/ Methyl green > I vaguely recall hearing that some animals produce > characteristic patterns when these stains are used - does anyone know of > any literature published, or is this one of those tools that gets used in- > house only? If Banse (1970) is any guide Methyl green staining was perhaps first used on polychaetes by Hofsommer (1913). Brigitte Hilbig's (1996) Cossura paper is a recent good example amongst several presenting staining patterns. Banse, K. 1970. The small species of Euchone Malmgren (Sabellidae, Polychaeta).— Proceedings of the Biological Society of Washington, 83(35):387-408. Hofsommer, A. 1913. Die Sabelliden-Ausbeute der Poseidon- Fahrten und die Sabelliden der Kieler Bucht.— Wissenschaftliche Meeresuntersuchungen, Kiel, neue Folge, 15:305-364. Hilbig, B. 1996. Family Cossuridae Day, 1963. Pp. 385-404 in J. A. Blake, B. Hilbig and P. H. Scott (eds.), Taxonomic Atlas of the Benthic Fauna of the Santa Maria Basin and Western Santa Barbara Channel. Volume 6. The Annelida Part 3. Polychaeta: Orbiniidae to Cossuridae, Vol. 6, Santa Barbara Museum of Natural History, Santa Barbara. -- Geoff Read -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Fri Oct 29 16:26:43 1999 Received: by net.bio.net (8.9.1a/8.9.1) id QAA08803; Fri, 29 Oct 1999 16:26:43 -0700 (PDT) Date: Fri, 29 Oct 1999 16:26:43 -0700 (PDT) Message-Id: <199910292326.QAA08803@net.bio.net> To: annelida@net.bio.net From: "Geoff Read" Reply-To: annelida@net.bio.net Subject: Re: Echiura > [...] I have no clear idea of what its sister-taxon would be. I hope > that will become clear as we study them, and the rest of the annelids. The REST of the annelids indeed! Has the second jury come back with a verdict already? The first verdict was overturned on appeal and a mistrial declared. :^) -- Geoff Read -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Sun Oct 31 01:23:22 1999 Received: by net.bio.net (8.9.1a/8.9.1) id BAA07760; Sun, 31 Oct 1999 01:23:22 -0800 (PST) Date: Sun, 31 Oct 1999 01:23:22 -0800 (PST) Message-Id: <199910310923.BAA07760@net.bio.net> To: annelida@net.bio.net From: "Geoff Read" Reply-To: annelida@net.bio.net Subject: Payraudeau book Hi ANNELIDA, Is anyone familiar with this? (offered by a dealer for lots & lotsa $$$!) What annelids are they? It looks as though it _might_ include marine annelids judging from the title. Hartman doesn't list it but Ward & Fauchald does. Payraudeau B.C., 1826 - Catalogue descriptif et méthodique des annelides et des mollusques de l'ile de Corse avec huit planches représentant quatre-vingt-huit esoéces, dont soixant-huit nouvelles. pp.218 -- Geoff Read -- ANNELIDA Discuss = annelida@net.bio.net = talk to all members Server = biosci-server@net.bio.net = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Sun Oct 31 12:53:13 1999 Received: by net.bio.net (8.9.1a/8.9.1) id MAA22935; Sun, 31 Oct 1999 12:53:13 -0800 (PST) Date: Sun, 31 Oct 1999 12:53:13 -0800 (PST) Message-Id: <199910312053.MAA22935@net.bio.net> To: annelida@net.bio.net Reply-To: annelida@net.bio.net From: Salva Subject: Last doubts Dear list-mates, After a lasting literature review, plus the help from individual researchers and Annelida members, I have been able to trace the reproductive modes of 80% of the species found in a polychate survey from the Canary Islands. I have a final list of species no-one seems to know/guess/have come accross/have explored into their reproducive strategy, whether planktotrophic (PLK), lecitotrophic (LEC) or direct development (DD). Does anyone know how do the following 10 polychaete taxa reproduce? (I attach my guess from refs from taxonomic relatives): 1/ (Amphinomidae) Chloeia viridis: DD, Kudenov 1977. 2/ (Amphynomidae) Eurythoe chilensis: no reference found. 3/ (Capitellidae) Capitomastus minimus: no reference found despite Jamieson & Rouse1989. May not even be a valid sp according to Linda Warren. 4/ (Chrysopetalidae) Bhawania reyssi: PLK, Blake 1975. 5/ (Eunicidae) Nematonereis unicornis: no reference found. ? LEC 6/ (Nereidae) Neanthes caudata and N. rubicunda: DD or LEC, Smith 1950, Fischer & Dhainaut 1985........LEC at a guess 7/ (Orbiniidae) Scolaricia sp.: LEC or DD, Anderson 1961, Gibbs 1968. Probably LEC 8/ (Paraonidae) Cirrophorus spp.: DD, Rasmussen 1973, Curtis 1977 9/ (Questidae) Questa caudicirra: unknown despite Jamieson & Rouse 1989 10/ (Serpulidae) Ditrupa arietina: unknown could be anything Thank you in advance. Salva ---------------------------------------------------------------------------- Working-@ addressses: salherra@ull.es / salherra@ctv.es _____________________________ Salvador Herrando-Perez Tenerife University, Spain ____________________________________________________ Temporal address until December 1999 Salvador Herrando-Pérez C/Padre Jofre 19, piso 3, pta 7 12006 Castellón de la Plana Spain telephone (34) 609 939201 fax 34 964 665074 (available from 8 am to 14 pm, Spanish timetable) -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html -- From daemon Sun Oct 31 14:40:17 1999 Received: by net.bio.net (8.9.1a/8.9.1) id OAA04552; Sun, 31 Oct 1999 14:40:17 -0800 (PST) Date: Sun, 31 Oct 1999 14:40:17 -0800 (PST) Message-Id: <199910312240.OAA04552@net.bio.net> To: annelida@net.bio.net Reply-To: annelida@net.bio.net From: bjamieson@zoology.uq.edu.au (Barrie Jamieson) Subject: Questa Re: Last doubts Dear Salva and list-mates, Insemination in Questa ersei Jamieson and Webb, 1984, is clearly by transference of intorsperm into an unpaired dorsal spermatheca in the female from a male apparatus which includes 'dorsal folds'. How the two copulate by means of these dorsal structures is not known but presumably they merely appose their dorsal surfaces (some enchytraeids and a few other oligochaetes also have dorsal spermathecae but then have ventrolateral male pores!). At the time I wrote, at least, questids were the only non-oligochaetes with a clitellum (differing from oligochaetes in being ANTERIOR to the female pores). Presumably eggs shed from the female pores are fertilized in the cocoon by egress of the allosperm from the spermatheca, as in most oligochaetes. However, one oligochaete family, the Eudrilidae, has spermathecal insemination and then internal fertilization - but there there are mechanisms for gettng the sperm to the eggs internally (in the simplest method by penetration of the wall of the spermathecae, in others by systems of tubes etc.) and I saw no evidence of this in Questa. The reference is: Jamieson, B.G.M. and Webb, R.I. 1984d. The morphology, spermatozoal ultrastructure and phylogenetic affinities of a new species of questid (Polychaeta; Annelida). In Proceedings of the First International Polychaete Conference, Sydney. Hutchings, P.A. ed. pp. 21-34. The Linnean Society of New South Wales, Sydney. Hope this helps to reduce your list. Best regards, Barrie Jamieson >I have a final list of species no-one seems to know/guess/have come >accross/have explored into their reproducive strategy, whether >planktotrophic (PLK), lecitotrophic (LEC) or direct development (DD). >9/ (Questidae) Questa caudicirra: unknown despite Jamieson & Rouse 1989 -- ANNELIDA LIST Discuss = = talk to all members Server = = un/subscribes Archives = http://www.bio.net:80/hypermail/ANNELIDA/ Resources = http://biodiversity.uno.edu/~worms/annelid.html --