From owner-acedb@net.bio.net Thu Feb 01 22:00:00 1996
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Newsgroups: bionet.software.acedb
Subject: protein and peptide objects in acedb
Message-ID: <3112631C.3514@zone.org>
From: Jason Martin <jason@zone.org>
Date: Fri, 02 Feb 1996 11:16:44 -0800
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Ace users, developers:

I am interested in storing exon translations from gene-prediction
programs as Protein/peptide objects.  We want to do this because of
the following sitatuation that can occur at exon splice boundaries:

     SPLICE
exon1  |  exon2
ACC GAG|GAT AGG
--- --- --- ---   the 'show translation' feature would give
THR GLU ASP ARG   the correct translation for exon2, and we would be :)

but occasionally we get this:

     SPLICE
exon1  |  exon2
C CGA G|GA TAG G   the 'show translation' feature would choke on this
- --- ---- --- -   because it doesn't know what frame to translate the
. ARG GLY   *  .   second exon in.

What we would like to do is store the translation directly as a
pepetide, that we could access by going to the 'corresponding_protein'
of each exon.  Once there, we could hopefully export the peptide
as an AA sequence.  I've tried to store peptides in an analagous
manner to storing DNA features, but there doesn't seem to be
any back-end built into aCEdb for displaying the translation.

Any ideas or help would be greatly appreciated. (sorry if you
didn't view this with a non-proportional font!)

Jason


---------------------------------------
Jason Martin
Biocomputational Scientist
Sequana Therapeutics, Inc.
11099 North Torrey Pines Rd. Suite 160
La Jolla, CA 92037

(619)-452-6550 
(619)-452-6653  [FAX]
jason@sequana.com
---------------------------------------

From owner-acedb@net.bio.net Fri Feb 02 22:00:00 1996
Path: biosci!GREENGENES.CIT.CORNELL.EDU!matthews
From: matthews@GREENGENES.CIT.CORNELL.EDU ("Dave Matthews")
Newsgroups: bionet.software.acedb
Subject: Re:  ACEDB3 -> ACEDB4.1
Date: 3 Feb 1996 13:47:42 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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> Also, are there other things I need to know when upgrading from
> 3.0 to 4.1? Has anyone written any documentation on upgrading?

Well, not really.  The best I know of is
<a href="ftp://probe.nalusda.gov/pub/acedocs/sampledb/">SampleDB</a>,
a small sample ace4 database that's supposed to function as "executable
documentation".

The main things you need to work on, as far as I recall, are:

1. Some of the magic tags that drive particular features of the GMAP
display have changed.  See SampleDB's models.wrm and the explanations in
<a href="http://probe.nalusda.gov:8000/acedocs/magic">Magic Tags</a> and 
<a href="http://greengenes.cit.cornell.edu/acedoc/GMAP.html">The Genetic Map 
Display</a>.

2. The GMAP display is now configurable, and configurations can be saved in
objects of class ?View.  See "The Genetic Map Display".  SampleDB contains
some Views that are better than the configuration you get if you don't use
a default View at all.

3. The traditional online help system now runs via Mosaic, using html
files in a new directory $ACEDB/whelp.  This requires a script in the new
directory wscripts.  See SampleDB.

4. Pick_me_to_call no longer assumes the data files are in $ACEDB/
externalFiles.  To invoke xv now on those same files now, replace
"Pick_me_to_call xv my.gif" with "Pick_me_to_call display my.gif" and create
the following executable script named $ACEDB/wscripts/display:

#!/bin/csh
xv $ACEDB/externalFiles/$1 &


- Dave

From owner-acedb@net.bio.net Fri Feb 02 22:00:00 1996
Path: biosci!ns1.faseb.org!lamarck.sura.net!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!lll-winken.llnl.gov!enews.sgi.com!decwrl!tribune.usask.ca!rover.ucs.ualberta.ca!mongol.sasknet.sk.ca!canopus.cc.umanitoba.ca!news
From: Brian Fristensky <frist>
Newsgroups: bionet.software.acedb
Subject: ACEDB3 -> ACEDB4.1
Date: 2 Feb 1996 21:19:25 GMT
Organization: University of Manitoba
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(I originally posted this message with no subject line. Sorry.)

I am trying to upgrade from ACEDB3 to ACEDB4.1, but when I start
ACEDB4.1, I get errors regarding my models. These models worked
in ACEDB3. The errors seem to fall into 3 classes:

###########################################################################

1) Model error: non-tag ?Journal in column in model ?Paper

//------------------------------------------------------------------
?Journal Paper ?Paper XREF Journal

//------------------------------------------------------------------
?Paper   Reference Title UNIQUE ?Text
                   Journal UNIQUE ?Journal XREF Paper
                                  Unpublished
                   Volume UNIQUE Text Text
                   Pages UNIQUE Text
                   Year UNIQUE Text
                   Publisher UNIQUE Text
         Author ?Author XREF Paper
         Contained_in ?Paper XREF Contains
                      Editor ?Author
         Medline_ID  UNIQUE Int
         GenBank     UNIQUE Text
         Accession UNIQUE Text
         Abstract ?LongText
         Type UNIQUE Text
         Contains ?Paper XREF Contained_in
         Image ?Image XREF Paper
         Refers_to  ANY
         Keyword ?Keyword
         Remark  Text
 
Journal does, in fact appear as a tag in tags.wrm.

###########################################################################

2) Model error: tag Strain outside rooted subtree in model ?DNA_Sample
//------------------------------------------------------------------
?DNA_Sample Name Text
            Experiment ?Experiment XREF DNA_Sample
            Image   ?Image
            Accession Text
            DNA_Type UNIQUE Plasmid ?Plasmid XREF DNA_Sample
                            Vector ?Vector XREF DNA_Sample
                            Genomic ?Species Strain Text
                            Linear_DNA ?Linear_DNA XREF DNA_Sample
            Conc.[ug_per_ul] Float
            Freezer Text
            Box Text

###########################################################################
3) Model error: In model ?Library, tag Paper XREF's to tag Library
             in class ?Paper
             can't find tag Library in model ?Paper

//------------------------------------------------------------------
?Library  Name Text // Full name
          Description ?Text // Description of material included
          Laboratory ?Laboratory
          Author ?Author
          Paper ?Paper XREF Library
          Experiment ?Experiment XREF Library REPEAT
          Donor_Species ?Species
                        Strain Text
          Library_Type UNIQUE cDNA
                              Genomic UNIQUE sheared
                                             Restriction_Enzyme Text
          Vector ?Vector
                 Restriction_Site Text Text
          Host_Strain ?Bacterial_Stock

Some class 3 errors are in models that are unchanged from the
models.wrm as distributed with ACEDB3.

Has model syntax changed, or is ACEDB4.1 just more fastidious at
detecting errors? 

Also, are there other things I need to know when upgrading from 
3.0 to 4.1? Has anyone written any documentation on upgrading?


-- 
===============================================================================
Brian Fristensky                |  
Department of Plant Science     |  A monarch's neck should always
University of Manitoba          |  have a noose around it --
Winnipeg, MB R3T 2N2  CANADA    |  it keeps him upright.
frist@cc.umanitoba.ca           |  
Office phone:   204-474-6085    |  Robert A. Heinlein
FAX:            204-261-5732    |
http://home.cc.umanitoba.ca/~frist/
===============================================================================


From owner-acedb@net.bio.net Fri Feb 02 22:00:00 1996
Path: biosci!ns1.faseb.org!lamarck.sura.net!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!lll-winken.llnl.gov!enews.sgi.com!decwrl!tribune.usask.ca!rover.ucs.ualberta.ca!mongol.sasknet.sk.ca!canopus.cc.umanitoba.ca!news
From: Brian Fristensky <frist>
Newsgroups: bionet.software.acedb
Subject: (no subject)
Date: 2 Feb 1996 21:06:41 GMT
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I am trying to upgrade from ACEDB3 to ACEDB4.1, but when I start
ACEDB4.1, I get errors regarding my models. These models worked
in ACEDB3. The errors seem to fall into 3 classes:

###########################################################################

1) Model error: non-tag ?Journal in column in model ?Paper

//------------------------------------------------------------------
?Journal Paper ?Paper XREF Journal

//------------------------------------------------------------------
?Paper   Reference Title UNIQUE ?Text
                   Journal UNIQUE ?Journal XREF Paper
                                  Unpublished
                   Volume UNIQUE Text Text
                   Pages UNIQUE Text
                   Year UNIQUE Text
                   Publisher UNIQUE Text
         Author ?Author XREF Paper
         Contained_in ?Paper XREF Contains
                      Editor ?Author
         Medline_ID  UNIQUE Int
         GenBank     UNIQUE Text
         Accession UNIQUE Text
         Abstract ?LongText
         Type UNIQUE Text
         Contains ?Paper XREF Contained_in
         Image ?Image XREF Paper
         Refers_to  ANY
         Keyword ?Keyword
         Remark  Text
 
Journal does, in fact appear as a tag in tags.wrm.

###########################################################################

2) Model error: tag Strain outside rooted subtree in model ?DNA_Sample
//------------------------------------------------------------------
?DNA_Sample Name Text
            Experiment ?Experiment XREF DNA_Sample
            Image   ?Image
            Accession Text
            DNA_Type UNIQUE Plasmid ?Plasmid XREF DNA_Sample
                            Vector ?Vector XREF DNA_Sample
                            Genomic ?Species Strain Text
                            Linear_DNA ?Linear_DNA XREF DNA_Sample
            Conc.[ug_per_ul] Float
            Freezer Text
            Box Text

###########################################################################
3) Model error: In model ?Library, tag Paper XREF's to tag Library
             in class ?Paper
             can't find tag Library in model ?Paper

//------------------------------------------------------------------
?Library  Name Text // Full name
          Description ?Text // Description of material included
          Laboratory ?Laboratory
          Author ?Author
          Paper ?Paper XREF Library
          Experiment ?Experiment XREF Library REPEAT
          Donor_Species ?Species
                        Strain Text
          Library_Type UNIQUE cDNA
                              Genomic UNIQUE sheared
                                             Restriction_Enzyme Text
          Vector ?Vector
                 Restriction_Site Text Text
          Host_Strain ?Bacterial_Stock

Some class 3 errors are in models that are unchanged from the
models.wrm as distributed with ACEDB3.Has model syntax changed, 
or is ACEDB4.1 just more fastidious at detecting errors? 

Also, are there other things I need to know when upgrading from 
3.0 to 4.1? Has anyone written any documentation on upgrading?


-- 
===============================================================================
Brian Fristensky                |  
Department of Plant Science     |  A monarch's neck should always
University of Manitoba          |  have a noose around it --
Winnipeg, MB R3T 2N2  CANADA    |  it keeps him upright.
frist@cc.umanitoba.ca           |  
Office phone:   204-474-6085    |  Robert A. Heinlein
FAX:            204-261-5732    |
http://home.cc.umanitoba.ca/~frist/
===============================================================================


From owner-acedb@net.bio.net Fri Feb 02 22:00:00 1996
Path: biosci!daresbury!not-for-mail
From: mieg@kaa.crbm.cnrs-mop.fr (Danielle et Jean Thierry-Mieg)
Newsgroups: bionet.software.acedb
Subject: model syntax:
Date: 3 Feb 1996 15:51:48 -0000
Lines: 125
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Distribution: bionet
Message-ID: <4f00ak$78o@mserv1.dl.ac.uk>
Original-To: net@admcnrs.cnrs-mop.fr


3 sort of problems were reported:

###########################################################################

1) Model error: non-tag ?Journal in column in model ?Paper

//------------------------------------------------------------------
?Journal Paper ?Paper XREF Journal

//------------------------------------------------------------------
?Paper   Reference Title UNIQUE ?Text
                   Journal UNIQUE ?Journal XREF Paper
                                  Unpublished
 
Journal does, in fact appear as a tag in tags.wrm.

###########################################################################

First of all, tags.wrm can be removed before constructing
a new database,
the file must stay if the database was constructed before 3.3
but really this does not apply to ace4 databases

also the construction:

  Journal UNIQUE ?Journal XREF Paper
                                  Unpublished
is not legal
in a given column one should not mix up tags and class objects

the corerect model should say

?Paper Reference Title UNIQUE ?Text
                 J UNIQUE  Journal ?Journal XREF Paper
                           Unpublished

in fact it is closer to real life


###########################################################################

2) Model error: tag Strain outside rooted subtree in model ?DNA_Sample
//------------------------------------------------------------------
?DNA_Sample Genomic ?Species Strain Text
            
###########################################################################

This is indeed illegal
only basic or constructed types may appear to the right of a pointer


you should say

?DNA_Sample Genomic ?Species #St

#St Strain Text

or you can have

?DNA_Sample Genomic ?Species ?Strain

###########################################################################
3) Model error: In model ?Library, tag Paper XREF's to tag Library
             in class ?Paper
             can't find tag Library in model ?Paper

//------------------------------------------------------------------
?Library  Name Text // Full name
          Description ?Text // Description of material included
          Laboratory ?Laboratory
          Author ?Author
          Paper ?Paper XREF Library
          Experiment ?Experiment XREF Library REPEAT
          Donor_Species ?Species
                        Strain Text
          Library_Type UNIQUE cDNA
                              Genomic UNIQUE sheared
                                             Restriction_Enzyme Text
          Vector ?Vector
                 Restriction_Site Text Text
          Host_Strain ?Bacterial_Stock


very true: you are trying to XREF to a non existing tag
this is clearly a bug


#####################################

The fatsest way to edit the models is to open emacs
edit the models file

and in a separate window to start tace and issue the command
read_model

this is much better for that task than using xace

####################################

ace 4 models support DateType , look in the session object to see
the date foramt

correect dates are for example

now
today
1996
1992-01-25
1995-01-25_18:11:37

..

in addition the coming 4.2 supports COORD modificator inside 
sequence models to indicate numbers which may slide when an 
insertion is made

say i insert a base in 200
then internally the code will edit every COORD > 200 to
increase by 1

there is no esxternal interface yet for this thing but it
is used by our sequence manipulation tools

Jean

From owner-acedb@net.bio.net Fri Feb 02 22:00:00 1996
Path: biosci!daresbury!not-for-mail
From: mieg@kaa.crbm.cnrs-mop.fr (Danielle et Jean Thierry-Mieg)
Newsgroups: bionet.software.acedb
Subject: models in ace 3 and ace4
Date: 3 Feb 1996 15:37:02 -0000
Lines: 9
Sender: lpddist@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <4evveu$6ee@mserv1.dl.ac.uk>
Original-To: net@admcnrs.cnrs-mop.fr


the model syntax has not changed
it is enriched a little but 100% compatible

BUT, it is more strict and carefully checks the xref
we then realised that ALL models used so far had glitches
in the way we were using XREF and became fully convinced of the 
interest of the checking !


From owner-acedb@net.bio.net Sun Feb 04 22:00:00 1996
Path: biosci!agate!howland.reston.ans.net!nntp.coast.net!news00.sunet.se!sunic!news99.sunet.se!news.kth.se!sibirien.physchem.kth.se!not-for-mail
From: staffan@biochem.kth.se (Staffan Bergh)
Newsgroups: bionet.software.acedb
Subject: Re: ACEDB3 -> ACEDB4.1
Followup-To: bionet.software.acedb
Date: 5 Feb 1996 08:19:42 +0100
Organization: Biochemistry, KTH, Stockholm
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Brian, you may already have had this answered by email, but as the news articles
in this group are now available as a searchable archive and the documantation is
lagging behind, I think it would be nice to have these comments available for
all:

In article <4etv4t$pl5@canopus.cc.umanitoba.ca> Brian Fristensky <frist> writes:

>I am trying to upgrade from ACEDB3 to ACEDB4.1, but when I start
>ACEDB4.1, I get errors regarding my models. These models worked
>in ACEDB3. The errors seem to fall into 3 classes:
>
>###########################################################################
>
>1) Model error: non-tag ?Journal in column in model ?Paper
>
>//------------------------------------------------------------------
>?Paper   Reference Title UNIQUE ?Text
>                   Journal UNIQUE ?Journal XREF Paper
>                                  Unpublished

				   ^ here you have tags and ?Objects mixed in
column, which is not allowed. There will be one more error like this from the
Paper model, after Contained_in

>                   Volume UNIQUE Text Text
>                   [some stuff deleted ...]
>         Contained_in ?Paper XREF Contains
>                      Editor ?Author
>         Medline_ID  UNIQUE Int
> [some more stuff deleted ...]
>
>Journal does, in fact appear as a tag in tags.wrm.

The tags.wrm, systags.wrm and sysmodels.wrm files are now obsolete, ACE
constructs the list of tags from the models.wrm file.

>###########################################################################
>
>2) Model error: tag Strain outside rooted subtree in model ?DNA_Sample
>//------------------------------------------------------------------
>?DNA_Sample Name Text
>            Experiment ?Experiment XREF DNA_Sample
>            Image   ?Image
>            Accession Text
>            DNA_Type UNIQUE Plasmid ?Plasmid XREF DNA_Sample
>                            Vector ?Vector XREF DNA_Sample
>                            Genomic ?Species Strain Text

					      ^ a tag after a ?Object is not
allowed, you have to use a constructed subtype

>###########################################################################
>3) Model error: In model ?Library, tag Paper XREF's to tag Library
>             in class ?Paper
>             can't find tag Library in model ?Paper

The model for ?Paper included does not have a Library tag

>
>Some class 3 errors are in models that are unchanged from the
>models.wrm as distributed with ACEDB3.

As Dave has already pointed out, there are some changes in the 'magic' tags
etc. Documentation on (at least some) of the magic stuff is at
	http://probe.nalusda.gov:8000/acedocs/index.html

>Has model syntax changed, or is ACEDB4.1 just more fastidious at
>detecting errors? 

ACEDB4.1 is much more picky. It now does error checking on the models ... Came
as a shock to me also when moving MycDB from 3 to 4!

Good Luck /staffan

Staffan Bergh
Biochemistry, KTH, S-100 44 Stockholm, Sweden

email: staffan@biochem.kth.se           + Don't let that horse eat that violin
phone: int+46 8 790 9230                +               cried Chagall's mother
fax: int+46 8 24 54 52                  + but he kept right on painting
                                        +             -- Lawrence Ferlinghetti

<A HREF="http://www.biochem.kth.se">Webmaster</A> and 
<A HREF="http://www.biochem.kth.se/MycDB.html">MycDB maintainer</A>

From owner-acedb@net.bio.net Mon Feb 05 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!gatech!news.emf.net!overload.lbl.gov!acedbfaq
From: acedbfaq@s27w007.pswfs.gov (ACEDB FAQ Pseudouser)
Newsgroups: bionet.software.acedb,news.answers
Subject: ACEDB Genome Database Software FAQ
Followup-To: bionet.software.acedb
Date: 6 Feb 1996 00:16:19 GMT
Organization: Dendrome, A genome database for forest trees
Lines: 1170
Approved: news-answers-request@MIT.Edu
Message-ID: <4f66kj$bo9@overload.lbl.gov>
Reply-To: acedbfaq@s27w007.pswfs.gov
NNTP-Posting-Host: s27w007.pswfs.gov
Summary: Frequently Asked Questions about finding and getting
 started with the database system ACEDB.  ACEDB is used
 to collect information regarding the molecular biology
 of the genome.
Xref: biosci bionet.software.acedb:838 news.answers:57802

Archive-name: acedb-faq
Last-modified: 2/5/96
Version: 1.27




ACEDB FAQ
*********


Curated by: Bradley K. Sherman 


Frequently Asked Questions about ACEDB
++++++++++++++++++++++++++++++++++++++

 o Q0 : What is ACEDB? 
 o --------------------
 o Q1 : What is the current version of ACEDB? 
 o -------------------------------------------
 o Q2 : What hardware/software do I need to run ACEDB? 
 o ----------------------------------------------------
 o Q3 : Where can I get ACEDB? 
 o ----------------------------
 o Q4 : !What ACEDB databases exist? 
 o ----------------------------------
 o Q5 : What documentation exists for ACEDB? 
 o ------------------------------------------
 o Q6 : What other resources are available for ACEDB? 
 o ---------------------------------------------------
 o Q7 : How should ACEDB be cited? 
 o --------------------------------
 o Q8 : Is ACEDB object-oriented? 
 o -------------------------------
 o Q9 : How does one get on/off the ACEDB announcements mailing list? 
 o -------------------------------------------------------------------
 o Q10 : When and where is the next ACEDB Workshop? 
 o -------------------------------------------------
 o Q11 : Who prepared this document & where is the current version? 
 o -----------------------------------------------------------------

Questions marked with '+' are new, those with '!' have substantially changed
answers. 


Q0: What is ACEDB?
++++++++++++++++++

A0:
+++

ACEDB is an acronym for A Caenorhabditis elegans Database. It can refer to a
database and data concerning the nematode C. elegans, or to the database
software alone. This document is concerned primarily with the latter meaning.
ACEDB is being adapted by many groups to organize molecular biology data about
the genomes of diverse species [Q4 gives contact information]. 

ACEDB allows for automatic cross-referencing of items during loading and allows
for hypertextual navigation of the links using a graphical user interface and
mouse. Certain special purpose graphical displays have been integrated into the
software. These reflect the needs of molecular biologists in constructing
genetic and physical maps of genomes. 

ACEDB was written and developed by Richard Durbin (MRC LMB Cambridge, England)
and Jean Thierry-Mieg (CNRS, Montpellier, France), beginning circa 1990. It is
written in the C programming language and uses the X11 windowing system to
provide a platform independent graphical user interface. The source code is
publicly available [See Q3]. Durbin & Thierry-Mieg continue to develop the
system, with contributions from other groups including Lawrence Berkeley
Laboratory and the Integrated Genomic Database (IGD) project headed by Otto
Ritter. 

A description by Durbin & Thierry-Mieg: ACEDB does not use an underlying
relational database schema, but a system we wrote ourselves in which data are
stored in objects that belong in classes. This is nevertheless a general
database management system using caches, session control, and a powerful query
language. Typical objects are clones, genes, alleles, papers, sequences, etc.
Each object is stored as a tree, following a hierarchical structure for the
class (called the "model"). Maps are derived from data stored in tree objects,
but precomputed and stored as tables for efficiency. The system of models allows
flexibility and efficiency of storage --missing data are not stored. A major
advantage is that the models can be extended and refined without invalidating an
existing database. Comments can be added to any node of an object. 

Return to List of Questions 


Q1: What is the current version of ACEDB?
+++++++++++++++++++++++++++++++++++++++++

A1:
+++

(This answer refers to the software not the C. elegans data.) 
Current version is 4_1 (as of 10 August 1995). 

A test (beta) version of ACEDB 4.0b4 (5/95) is available. 

A Macintosh version is available as version 2.0b4. 

To retrieve the software see Q3. 
To be kept informed of new releases see Q9. 

Return to List of Questions 


Q2: What hardware/software do I need to run ACEDB?
++++++++++++++++++++++++++++++++++++++++++++++++++

A2:
+++

The software is available as source code, so you may be able to get it working
on any machine, with effort. It is also available in binary (pre-compiled)
format for a variety of machines. To retrieve the software see Q3. 

 o Unix and X11: 
    o Sun/SunOS 4.x 
    o Sun/Solaris 
    o DEC DECstation3100, 5100 etc. 
    o DEC Alpha/OSF-1 
    o Silicon Graphics Iris series 
    o PC 386/486/Pentium with Linux (free Unix) Jeff Bryer,
      jbryer@darwin.mbb.sfu.ca and Ken Clark provide a linux version of 4.1 and
      a complete C. elegans data set at trog.mbb.sfu.ca in /pub/acedb as
      bin.linux.4_1.tar.Z and in ELF binary as 
      ftp://trog.mbb.sfu.ca/pub/acedb/bin.linux.4_1.elf.tar.gz . 
    o There exist, or have existed, ports onto Alliant, Hewlett- Packard, IBM
      R6000, Convex. You may have to contact the developer responsible for the
      port to make these real. 
    o NeXT: contact Patrick Phillips at University of Texas, NeXTmail:
      patrick@wbar.uta.edu email: phil@decster.uta.edu 
 o Macintosh: 
    o [Contributed by Frank Eeckman] Macace is distributed as a self-extracting
      archive that contains the application, the wspec files, and a fully up to
      date database. macace 3.0 is available with an updated 21bdb database.
      Please send all questions/bug reports to eeckman@llnl.gov A native powerPC
      version is available as well. Macace needs a macintosh with > 16 MBytes of
      RAM, and a 17" color monitor is preferred. System 7 or greater is
      required. For the multimedia extensions Quicktime 1.0 is required. Please
      add your name to our mailing list by sending email to eeckman@llnl.gov. It
      is our belief that for cost savings a powerPC mac will beat the advertised
      linux-intel combination. Macace is fully compatible with xace, but
      includes some multimedia extensions (picture and movie support) not found
      in the unix versions. 
 o ACEDB for The NEC EWS4800 is available via anonymous ftp at ftp.nec.co.jp
   (192.135.93.2) in /pub/packages/acedb/ace2. Contact Tohru Sano Fundamental
   Research Laboratories NEC Corporation Tsukuba, 305, JAPAN, e-mail:
   sano@exp.cl.nec.co.jp, FAX: +81-298-56-6136, VOICE: +81-298-50-1507 

(Here at the Institute of Forest Genetics we run ACEDB on a Sun Microsystems
SPARCstation II, and users can interact using Macintoshes and PC-clones by using
X11 implementations for the personal computers and a LAN. --bks) 

X11 fonts note: ACEDB uses fonts listed in the xfonts.wrm file. If you install
new fonts on your machine be sure to run bldfamily(1) so that they are
available. 

Return to List of Questions 


Q3: Where can I get ACEDB?
++++++++++++++++++++++++++

A3:
+++

The standard ACEDB source and binaries are available in the following public
access accounts (anonymous ftp sites) accessible via Internet: 

 o lirmm.lirmm.fr in pub/acedb 
 o cele.mrc-lmb.cam.ac.uk in pub/acedb 
 o ncbi.nlm.nih.gov in repository/acedb 
 o bioinformatics.weizmann.ac.il in pub/databases/acedb. 

MacAce is available from: 

 o genome.lbl.gov in pub/macace 
 o cele.mrc-lmb.cam.ac.uk in pub/acedb/macace 

Linux; ACEDB version 4.1 (and earlier versions) for Linux 2-10: 

 o trog.mbb.sfu.ca /pub/acedb as bin.linux.4_1.tar.Z. 

ACEDB for The NEC EWS4800: 

 o ftp.nec.co.jp in /pub/packages/acedb/ace2. 

Return to List of Questions 


Q4: What ACEDB databases exist?
+++++++++++++++++++++++++++++++

A4:
+++

In alphabetic order by Database name
------------------------------------

[Curators, please submit changes as new paragraphs] 

Database : AaeDB
Species : Aedes aegypti (Mosquito)
Last_update : December 1994
ACEDB_version : 3.0
WWW : http://klab.agsci.colostate.edu/
Curator : Dennis Knudson, dknudson@lamar.colostate.edu
Curator : Martin Ferguson, martinf@lamar.colostate.edu
Co-Curator : Dave Severson, dave@aedes.vetsci.wisc.edu
Co-Curator : Leonard Munstermann, munst@biomed.med.yale.edu
Contact : aaedbmgr@klab.agsci.colostate.edu

Database : AAtDB
Species : Arabidopsis thaliana
Current version : 3-5
Last_update : August 1995
PI : J. Michael Cherry
Curator : David Flanders
Contact : arab-curator@genome.stanford.edu
Availability : UNIX and Macintosh versions via anonymous ftp
FTP : genome-ftp.stanford.edu in arabidopsis/aatdb
FTP : ncbi.nlm.nih.gov in repository/aatdb
FTP : probe.nalusda.gov in pub/aatdb
Gopher : genome-gopher.stanford.edu/11/Arabidopsis
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://genome-www.stanford.edu
WWW : http://probe.nalusda.gov:8300/plant/ 

Database : AboutDB
Curator : Staffan Bergh, staffan@biochem.kth.se
Subject : ACEDB itself (meta-meta-metadata)
ACEDB_version: 3.0
Last_update : July 1995
WWW : http://www.biochem.kth.se/AboutDB.html 

Database : ACeDB
Species : Caenorhabditis elegans
Current version: 4-8
Curator : Jean Thierry-Mieg, mieg@kaa.crbm.cnrs-mop.fr
Curator : Richard Durbin, rd@mrc-lmb.cam.ac.u
Curator : Sylvia Martinelli, sylvia@sanger.ac.uk
Last_update : August 1995
Availability: Unix and Macintosh versions via anonymous ftp
FTP: USA - ncbi.nlm.nih.gov in repository/acedb
FTP: England - ftp.sanger.ac.uk in pub/acedb
FTP: France - lirmm.lirmm.fr in genome/acedb
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/other/ 

DataBase : AceMap
Species : Homo sapiens
Subject : Physical mapping data for human chromsome X
ACEDB_version : 4.1
Curator : Hugues Roest Crollius, croillius@mpimg-berlin-dahlem.mpg.de
PI : Hans Lehrach
FTP : ftp.icnet.uk in icrf-public/GenomeAnalysis/X/acemap
Comment: Get the README file in the directory above.
Contact : Max-Planck-Institut fur Moleulare Genetic, Ihnestrasse 73, 14195
Berlin, Germany
Availability : beta release of the X chromosome data/models via anonymous ftp
FTP : ftp.icnet.uk in icrf-public/GenomeAnalysis/X/acemap 
Last_update : August 1994 

Database : AGsDB (A Genus species Database)
Species : Aspergillus nidulans
Species : Neurospora crassa
Species : Bos taurus (cow)
Species : Homo sapiens anchor loci
Species : Gossypium hirsutum (cotton)
Species : Neurospora crassa
Species : Homologs of Aspergillus cell cycle loci for budding and fission yeast
Curator : Leland Ellis, leland@straylight.tamu.edu
Last_update : March 1994
ACeDB_version : 3.0
Subject: Contains extensions to the Human C21 Models to provide for multiple
species, and queries between species via Homologs (e.g., cell cycle loci with
links via Homologs between Aspergillus and budding C. cerevisiae) and fission
(S. pombe yeast); interacting loci via defined Interactions for each locus
Revision : AAnDB for Aspergillus nidulans and ABtDB for Bos taurus (cow) have
been folded into AGsDB, and are not being developed futher as individual species
databases.
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/other/
WWW : http://keck.tamu.edu/cgi/agsdb/agsdbserver.html 

Database : Alfagenes
Species : Medicago sativa (alfalfa)
Curator : D. Z. Skinner, dzolek@ksu.ksu.edu
Telephone : (913) 532-7247
ACEDB_version : 3.0
Last_Update : July 1995
FTP : probe.nalusda.gov in pub/alfagenes
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/plant/ 

Database : BeanGenes
Species : Phaseolus and Vigna
Curator : Phillip E. McClean, mcclean@beangenes.cws.ndsu.nodak.edu
ACEDB_version : 1.0
Last_updated : September 1995
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/plant/ 

Database : ChlamyDB
Species : Chlamydomonas
Curator : Elizabeth Harris
Contact : chlamy@acpub.duke.edu
ACEDB_version : 3.0
Data_version : 1.2
Last_update : August 1995
Availability : Macintosh and UNIX versions via anonymous ftp
FTP : probe.nalusda.gov in pub/chlamydb
Gopher : ftp.duke.edu/11/pub/chlamy
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/plant/ 

Database : CIMMYT (Wheat International Nursery Data)
Species : Triticum spp.
ACEDB_version : 4.0
Last_update : September 1995
Curator : Hector Sanchez, hsanchez@cimmyt.mx
FTP : probe.nalusda.gov in pub/cimmyt
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/related/ 

Database : CoolGenes
Species : Cool Season Fool Legumes; Pisum, Lens, Cicer, Lathyrus, Vicia faba
Curator : Fred Muehlbauer, muehlbau@wsu.edubr> ACEDB_version : 3.0
Last_update : January 1996
Gopher : gopher://probe.nalusda.gov:7000/11/genome.databases/coolgenes/
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/plant/ 

Database : CottonDB
Species : Gossypium hirsutum (cotton) and related species
PI : Russell J. Kohel (rjk0339@acs.tamu.edu), USDA-ARS, Southern Crops Research
Laboratory, 2765 F&B Road, College Station, Texas 77845
Curator : Gerard R. Lazo, lazo@tamu.edu
Curator : Sridhar Madhavan, msridhar@tamu.edu
Phone : 409-260-9311
Fax : 409-260-9333
Last_update : January 1995 (version 95.1)
ACEDB_version : 3.0
FTP : probe.nalusda.gov in pub/cottondb
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/plant/
Data_submission_form : http://algodon.tamu.edu/ 

Database : CSNDB
Focus : Cell Signalling Molecules and Interactions
Contact : Takako Igarashi, National Insitute of Health Sciences, Division of
Chem-Bio Informatics, Setagaya-ku, Tokyo, Japan 158, taka@nihs.go.jp 

Database : EofD (Encyclopaedia of the Drosophila)
Species : Drosophilidae (primarily D. melanogaster)
Availability : Macintosh CD-ROM and SunOS executables via anonymous ftp
Last_update : November 1995
ACEDB_version : 4.1
Developer : Suzanna Lewis, suzi@fly2.berkeley.edu
Developer : Cyrus Harmon, sly@fly2.berkeley.edu
Developer : Edward Welbourne, eddy@gen.cam.ac.uk
Collaborator : The FlyBase Consortium, flybase-help@morgan.harvard.edu
Collaborator : The Berkeley Drosophila Genome Project,
flygenome@maillink.berkeley.edu
FTP : fruitfly.berkeley.edu in pub/EofD
Gopher : fly.bio.indiana.edu:70+/11/Flybase
WWW : http://shoofly.berkeley.edu
WWW : http://fly2.berkeley.edu:3512/EofD.html

Database : EthnobotDB (orldwide plant uses)
Species : wide range of plant species
ACEDB_version : 4.0
Last_update : June 1995
Comment : Converted to ACEDB from the original SQL database.
Curator : Stephen M. Beckstrom-Sternberg, peosb@ars-grin.gov 
Curator : James A. Duke, ngrljd@ars-grin.gov
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/related/ 

Database : FoodplantDB (Native American Food Plants)
Species : Over 1,100 plant species
ACEDB_version : 4.0
Last_update : May 1995
Curator : Stephen M. Beckstrom-Sternberg, peosb@ars-grin.gov 
Curator : James A. Duke, ngrljd@ars-grin.gov
Comment : Converted to ACEDB from ORACLE.
Comment : Data originally from a publication by Yanovsky, Elias. 1936. Food
Plants of the North American Indians. USDA Miscellaneous Publication Number 237.
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/related/ 

Database : GrainGenes
Species : Wheat, barley, oats, relatives
Curator : David E. Matthews, matthews@greengenes.cit.cornell.edu
PI : Olin D. Anderson, oandersn@pw.usda.gov
ACEDB_version : 4_1
Data_version : 1.5+
Last_update : November 1995
FTP : probe.nalusda.gov in pub/graingenes
Gopher : greengenes.cit.cornell.edu/
Gopher : probe.nalusda.gov:7002
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/plant/
WWW : http://wheat.pw.usda.gov/graingenes.html 

Database : IGD (Integrated Genomic Database)
Species : Homo sapiens
Subject : Chromosome 21
Availability : September 1994 by ftp, on-line server October 1994
Contact : Otto Ritter, o.ritter@dkfz-heidelberg.de
Contact : Jean Thierry-Mieg, mieg@kaa.cnrs-mop.fr
Contact : Nicole Creau-Goldberg, creau@arthur.citi2.fr
Contact : Jean-Maurice Delabar, delabar@arthur.citi2.fr
WWW : http://moulon.inra.fr/acedb/igd.html
Description : IGD (Integrated Genomic Database) aims to integrate multiple
public general molecular biology and human genome specific databases into single
logical database with unified interface to existing analysis tools. From data
produced by the 4th International Workshop on Chromosome 21
(Genomics,1993,18,735-744) and from data provided by or taken from the following
databases and data repositories: GDB, OMIM, EMBL, CEPH, Genethon, UKProbeBank,
and RLDB. 

Database : LeishDB
Species : Leishmaia major, L. infantum, L. peruviana, L. donovani and others
PI : Jennie Blackwell
PI : Al Ivens
Last_update : September 1995
ACEDB_version : 4.1
Contact : Matin Aslett, ma10025@cus.cam.ac.uk
FTP : ftp://parsun1.path.cam.ac.uk
WWW : http://parsun1.path.cam.ac.uk/
Curator : Martin Aslett
Curator : Howard Cobb

Database : LIGM-DB
Curator : Veronique Giudicelli
Focus : genetic and physical mapping of loci of Immunoglobulins and Tcell
receptors
PI : Marie-Paule Lefranc
Contact : Veronique Giudicelli LIGM IGMM UMR CNRS 9942 BP 5051 Rte de Mende
34000 Montpellier giudi@ligm.crbm.cnrs-mop.fr 

Database : MaizeDB
Species : Zea mays L. ssp. mays and related species
Latest_release : April 1995
Acedb_version : 3.3
Comment : MaizeDB is a periodically extracted ACeDB front end for the Maize
Genome Database, MaizeDB, a SYBASE database, implemented using Genera software,
developed by Stan Letovsky.
Comment : Genera provides (1) up-to-the-minute form internet access to SYBASE
databases, both for query (WWW and APT) and data entry (APT only); (2) database
design alterations by systems administrators; (3) gopher file extraction.
Comment: MaizeDB uses WWW record-to-record hard-links to 16 external databases
for (1) annotated sequences: GenBank, dbEST, GenoBase, PIR, SwissProt, Prosite,
ENZYME; (2) other species genome information: AAtDB(Arabidopsis), RiceGenes,
GrainGenes, SaachDB (yeast), CGSC(E. coli), XLocus, RiceGenes, GrainGenes (3)
germplasm: GRIN. 
Data : Over 100,000 records include: 6513 mapped loci (located to chromosome or
better) including 1081 mapped genes and 1937 mapped probed sites (gene
candidates); 2448 probes; 2460 map scores; 1551 gel patterns
(Probe/Enzyme/Stock); 8777 stocks; 18,100 Variations (alleles, DNA
polymorphisms, rearrangements, etc); 596 phenotypes; 317 traits; 715 gene
products; 6158 bibliographic references; 2200 researchers with addresses.
FTP : probe.nalusda.gov in pub/maizedb
Gopher : gopher.agron.missouri.edu
Gopher : probe.nalusda.gov:7000/11/genome.databases/
Telnet : telnet teosinte.agron.missouri.edu login as guest, use password:
corncob
WWW : http://www.agron.missouri.edu/top.html
WWW : http://probe.nalusda.gov:8300/plant/
WWW Genera information : http://gdbdoc.gdb.org/letovsky/genera/genera.html
Funding : MaizeDB USDA/ARS to E. Coe
Funding : Genera NSF BIR 92-01652 to S. Letovsky and M. Berlyn
Curator/PI : Ed Coe, ed@teosinte.agron.missouri.edu
Curator : Mary Polacco, maryp@teosinte.agron.missouri.edu
Assoc Curator : Pat Byrne, byrne@teosinte.agron.missouri.edu QTL data
Assoc Curator : Georgia Davis, gdavis@teosinte.agron.missouri.edu Map data
Assoc Curator : Marty Sachs, Maize Stock Center, msachs@uiuc.edu Genetic Stock
data
Assoc Curator : Christiane Fauron, FAURON@GENE1.med.utah.edu Mitochondrion
genome
Assoc Curator : Carolyn Wetzel, cmwetzel@iastate.edu Chloroplast genome
Assoc Curator : Steve Rodermel, S1SRR@ISUVAX.IASTATE.EDU Chloroplast genome
Design : Stan Letovsky, letovsky@gdb.org
Design : Mary Berlyn, mary@fetalpig.biology.yale.edu
Systems Manager : Denis Hancock, dhancock@teosinte.agron.missouri.edu
Contact : db_request@teosinte.agron.missouri.edu
Last_update : 11 April 1995 

Database : Mendel (plant wide gene names)
Species : wide range of plant species
Subject : standardized designations for sequenced genes
Comment : The purpose is to provide a common system of nomenclature for
substantially similar genes across the plant kingdom. Mendel is maintained by
the Commission on Plant Gene Nomenclature.
ACEDB_version : 4.0
Last_update : July 1995
Curator : Carl Price, price@mbcl.rutgers.edu
Curator : Ellen Reardon, reardon@mbcl.rutgers.edu
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/related/ 

Database : Millet Genes
Species : Pennisetum Glaucum (Pearl Millet)
ACEDB_version : 4.1
Availablity : Local
Curator : Matt Couchman, couchman@bbsrc.ac.uk
Curator : Katrien Devos, devos@bbsrc.ac.uk

DataBase : Mousedb
Species : Musculus Musculus
Species : Homo Sapiens
ACEDB_version : 3.0 with extensions to define and display cytogenetic data.
Description : Mouse genome data from the published literature, including mouse
genes with phenotypic effects, chromosome anomalies, imprinted regions and
man-mouse homologies with associated pathological disorders. The maps are
consensus ones. They use data, such as the HIS and anomaly data, to show
alignments between the genetic and cytogenetic maps.
Curator : Rachael Selley, rselley@har-rbu.mrc.ac.uk
PI : Mary Lyon
PI : Jo Peters
Availability : Mousedb is available publicly from the UK HGMP Resource Centre's
computing service via the INTERNET. For user id. please contact Administration,
HGMP Resource Centre, Hinxton Hall, Cambridgeshire CB10 1RQ, UK. 
Tel: (+44) 1223 494520 Fax: (+44) 1223 494510 
Contact : Rachael Selley, MRC Radiobiology Unit, Chilton, Didcot, Oxon OX11 ORD
Last_update : July 1995 

Database : MPNADB (Medicinal Plants of Native America)
Species : Over 2,100 plant species
Curator : Daniel E. Moerman, dmoerman@umich.edu
Curator : Stephen M. Beckstrom-Sternberg, peosb@ars-grin.gov - ACEDB version 
Comment : MPNADB is based on a two-volume book of the same name published in
1986 by the Museum of Anthropology of the University of Michigan. MPNADB was
first developed at the University of Michigan in DBase II.
ACEDB_vertion : 4.0
Last_update : June 1995
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/related/ 

Database : MycDB
Species : Mycobacteria
Comment : MycDB is a collation of data on the mycobacteria, causative agents of
tuberculosis and leprosy. It is centered on the mapping and sequencing projects
under way in M.leprae and M.tuberculosis.
Curator : Staffan Bergh, staffan@biochem.kth.se
Curator : Stewart Cole, stcole@pasteur.fr
Last_update : November 1995
ACEDB_version : 4.1
Data_version : 4-9 (November 1995)
FTP : www.biochem.kth.se (130.237.52.64) in pub/MycDB
FTP : ftp.pasteur.fr (157.99.64.12) in pub/MycDB
FTP : bioinformatics.weizmann.ac.il (132.76.55.12) in pub/databases/acedb/mycdb
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://www.biochem.kth.se/MycDB.html
WWW : http://probe.nalusda.gov:8300/other/ 

Database : OMIA (Online Mendelian Inheritance in Animals)
Species : wide range of animal species
Subject : gene and phene (familial trait or phenotype) information
Comment : MIA is modeled after Victor McKusick's Mendelian Inheritance in Man
(MIM) database and was developed at the University of Sydney, Australia, in
Advanced Revelation.
Curator : Frank Nicholas, frankn@doolittle.vetsci.su.oz.au
Curator : Gail Juvik, gjuvik@nalusda.gov - ACEDB version
ACEDB_vertion : 4.0
Last_update : September 1995
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/animal/
WWW : http://morgan.angis.su.oz.au/BIRX/phenes_form.html 

Database : PhytochemDB (Plant Chemicals)
Species : wide range of plant species
Subject : Consists primarily of plant chemical data, including quantity,
taxonomic occurence, and chemical activity.
Comment : Converted to ACEDB from the original SQL database.
ACEDB_version : 4.0
Last_update : June 1995
Data_version : July 1994
Curator : Stephen M. Beckstrom-Sternberg, peosb@ars-grin.gov 
Curator : James A. Duke, ngrljd@ars-grin.gov
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/related/ 

Database : PomBase
Curator : Sean Walsh, svw@sanger.ac.uk
Curator : Marie-Adele Rajendream
PI : Bart Barrell, barrell@sanger.ac.uk
Species : Schizosaccharomyces pombe
ACEDB_version : 4.1
Last_update : September 1995
FTP : ftp.sanger.ac.uk in pub/PomBase 

Database : PVP (Plant Variety Protection)
Species : Glycine max (soybeans)
Subject : Data about plant varieties that have been granted a Certificate of
Protection by the Plant Variety Protection Office.
Curator : Stephen M. Beckstrom-Sternberg, sbeckstr@nalusda.gov - ACEDB version
ACEDB_version : 4.0
Last_update : June 1995
Contact: The Plant Variety Protection Office, Room. 500, National Agriculture
Library, 10301 Baltimore Blvd., Beltsville, Maryland 20705
Telephone : 301-504-5518
Fax : 301-504-5291
Email : Jeff Strachan, strachan@locus.nalusda.gov
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/related/ 

Database : RiceGenes
Species : Oryza sativa
Curator : Edie Paul, epaul@nightshade.cit.cornell.edu
PI : Susah McCouch
ACEDB_version : 4.1
Last_update : November 1995
FTP : probe.nalusda.gov in pub/ricegenes
Gopher : nightshade.cit.cornell.edu
Gopher : probe.nalusda.gov:7007
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/plant/ 

Database : SacchDB
Species : Saccharomyces cerevisiae
Subject : Budding (common baker's) Yeast Genome
ACEDB_version : UNIX 2.0, MacAce 2.0b4
Last_update : September 1995
Data_version : 2.4.12
Data : All Saccharomyces genes contained in the Registry of Gene Names. Results
of the completed chromosomal sequencing projects have been integrated into the
database. Physical Maps based on DNA sequencing projects, hybridization to the
Olson/Riles prime filter grids, and restriction mapping. For the completely
sequenced chromosomes the Olson prime clones have been re-mapped (on the
computer) to the DNA sequence. Saccharomyces DNA sequences contained within
GenBank are incorporated. Literature references, most including abstracts, for
the information contained within the database. Gene protein product information
obtained from the YPD database (Garrels and Latter, CSHL) and the literature.
Genetic Maps including the underlying two point tetrad data. Including all
tetrad data reported in previous additions of the Mortimer Yeast Maps.
FTP : genome-ftp.stanford.edu in pub/yeast/SacchDB
FTP : ncbi.nlm.nih.gov in repository/SacchDB
Gopher : genome-gopher.stanford.edu
WWW : http://genome-www.stanford.edu/
Funding : National Center for Human Genome Research, NIH
PI : David Botstein, botstein@genome.stanford.edu
Project Manager/Curator : Mike Cherry, cherry@genome.stanford.edu
Curator : Selena Dwight, dwight@genome.stanford.edu
Curator : Cathy Ball, ball@genome.stanford.edu
Curator : Rita Schmidt, bleb@genome.stanford.edu
Curator : Barbara Dunn, bdunn@genome.stanford.edu
Curator : Caroline Adler, adler@genome.stanford.edu
Programmer : Karen Davis, karen@genome.stanford.edu
Sys. Admin : Mark Schroeder, mark@genome.stanford.edu
Contact : yeast-curator@genome.stanford.edu
Data_Submission : yeast-curator@genome.stanford.edu 

Database : SolGenes
Subject : Solanaceae - tomato, potato, pepper
Curator : Clare Nelson, cnelson@nightshade.cit.cornell.edu
PI : Steve Tanksley
Release : ACEDB 3.0
Last_update : May 1995
FTP : probe.nalusda.gov in pub/solgenes
Gopher : nightshade.cit.cornell.edu:71
Gopher : probe.nalusda.gov:7006
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/plant/ 

Database : SorghumDB
Species : Sorghum bicolor (L.) Moench
PI : Keith F. Schertz, schertz@tamvm1.tamu.edu
USDA-ARS, Dept. of Soil & Crop Sciences, Texas A&M University, College Station,
TX 77843-2474
Phone : (409) 260-9252
FAX : (409) 845-0456
Curator : Najeeb U. Siddiqui, nus6389@tam2000.tamu.edu
Southern Crop Improvement Facility, Crop Biotechnology Center, Texas A&M
University, College Station, TX 77843-2123
Phone : (409) 862-1523
FAX : (409) 862-4790
Last_update : September 1995
ACEDB_version : 3.0
Data_version : 2.0
FTP : probe.nalusda.gov in pub/sorghumdb
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/plant/ 

Database : SoyBase
Species : Glycine max (Soybeans) and related species
PI : Randy Shoemaker
Curator : David Grant, dgrant@iastate.edu
Assistant_curator : Marica Imsamde, mimsande@mendel.agron.iastate.edu
Contact : David Grant, dgrant@iastate.edu
ACEDB-Version : 3.7
Last_update : October 1995
FTP : probe.nalusda.gov in pub/soybase
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/plant/
WWW : http://mendel.agron.iastate.edu:8000/main.html 

Database : Syndb
Species : Homo sapiens, Mus musculus
Subject : STS content mapping & directed sequencing of Human Chromosomes 21,5
with Mouse for syntenic comparison
ACEDB_version : acedb v3.3 plus moulon server
Last_update : October 1994
FTP : genome.lbl.gov in pub/acedb/
WWW : http://genome.lbl.gov/Genome/acepage.html
Curator : Donn F. Davy, DFDavy@lbl.gov
Contact : Arun K. Aggarwal, aggarwal@genome.lbl.gov
PI : Michael Palazzolo
PI : Chris Martin
PI : Jan-Fang Cheng, jcheng@genome.lbl.gov 

Database : Toxo
Species : Toxoplasma gondii
PI : Jennie Blackwell
PI : Jim Ajioka
Last_update : September 1995
ACEDB_version : 4.1
Contact : Martin Aslett, ma10025@cus.cam.ac.uk
FTP : parsun1.path.cam.ac.uk
WWW : http://parsun1.path.cam.ac.uk/
Curator : Martin Aslett
Curator : Howard Cobb

Database : TreeGenes
Species : Forest trees
ACEDB_version : 3.0
Last_update : July 1995
Curator : Bradley K. Sherman, bks@s27w007.pswfs.gov
PI : David B. Neale, dbn@s27w007.pswfs.gov
Contact : Dendrome@s27w007.pswfs.gov
FTP : probe.nalusda.gov in /pub/treegenes
Gopher : s27w007.pswfs.gov/
Gopher : probe.nalusda.gov:7508/
Gopher : probe.nalusda.gov:7000/11/genome.databases/
WWW : http://probe.nalusda.gov:8300/plant/
WWW : http://s27w007.pswfs.gov/ 

Database : Trypbase
Species : Trypanosoma brucei
PI : Sara Melville
Curator : Martin Aslett, ma10025@cus.cam.ac.uk
Curator : Howard Cobb, hc102@econ.cam.ac.uk
Contact : sm160@mole.bio.cam.ac.uk
ACEDB_version : 4.1 (Sun and Linux)
FTP : parsun1.path.cam.ac.uk
WWW : http://parsun1.path.cam.ac.uk 

Database : 21Bdb
Species : Homo sapiens
Subject : STS content mapping and sequencing of Human Chromosome 21
ACEDB_version : acedb.1-10 plus moulon server
Curator : Donn F. Davy, DFDavy@lbl.gov
Contact : Arun K. Aggarwal, aggarwal@genome.lbl.gov
PI : Michael Palazzolo
PI : Chris Martin
PI : Jan-Fang Cheng, jcheng@genome.lbl.gov
Last_update : April 1994
FTP : ftp://genome.lbl.gov in pub/acedb/
WWW : http://genome.lbl.gov/Genome/acepage.html 

Database : 11Db
Species : Homo sapiens
Subject : Physical and Genetic mapping of Human Chromosome 11 Description : 11Db
attempts to show as full a picture as possible of the genetic and physical maps
of Human Chromosome 11. It has two new displays, one which attempts to integrate
as much of the mapping data as possible using minimal intervals, and one which
displays YAC Contigs downloaded from SEGMAP datafiles.
Contact : Benedict Arnold (b.arnold@bc.ic.ac.uk)
PI : Peter Little (p.little@bc.ic.ac.uk)
ACEDB_version : based on 4_1 with added map displays.
ACEDB_version : based on 3_6 with added map displays.
Data version : 1.0
Last Update : December 1995
WWW : http://chr11.bc.ic.ac.uk
FTP : ftp.cc.ic.ac.uk
Description : 11Db attempts to show as full a picture as possible of the genetic
and physical maps of Human Chromosome 11. It has two new displays, one which
attempts to integrate as much of the mapping data as possible using minimal
intervals, and one which displays YAC Contigs downloaded from SEGMAP datafiles.

Database : 22ace
Species : Homo sapiens
Subject : Physical map of human chromosome 22, genomic sequencing and more
ACEDB_version : 4.1
Curator : Ian Dunham, id1@sanger.ac.uk
Curator : Gareth Maslen, glm@sanger.ac.uk
PI : Ian Dunham
Last_update : August 1995
FTP : ftp.sanger.ac.uk in pub/human/chr22/physical_map/
WWW : http://www.sanger.ac.uk/hum22/

Database : VoxPop
Species : Populus species
Curator : Carl G. Riches, cgr@poplar1.cfr.washington.edu
PI : Reinhard F. Stettler, STETTLER@coyote.cfr.washington.edu
Last_update : September 1993
ACEDB_version : 1.9
FTP : poplar1.cfr.washington.edu in /pub/
Gopher : poplar1.crf.washington.edu 

Return to List of Questions 


Q5: What documentation exists for ACEDB?
++++++++++++++++++++++++++++++++++++++++

A5:
+++

From Sam Cartinhour: The ACEDB Documentation Server is a repository for
documentation concerned with "A C. elegans Data Base", the generic genome
database software designed by Richard Durbin (MRC, UK) and Jean Thierry-Mieg
(CNRS, France). The server is intended as a resource for developers, curators,
and end-users of all (not just plant) databases derived from ace. Eventually we
hope to offer all kinds of documentation, from reprints to (technical) gossip.
The ACEDB documentation server is sponsored by the Plant Genome Database Project
at the National Agricultural Library (USDA). The documentation server is listed
on the home page for the Agricultural Genome World Wide Web Server at 
http://probe.nalusda.gov:8000 . 

Primary documents from the developers are: 

 o acedb -- A C. elegans Database: I. Users' Guide. 
 o acedb -- A C. elegans Database: II. Installation Guide. 
 o acedb -- A C. elegans Database: III. Configuration Guide. 
 o Syntactic Definitions for the ACEDB Data Base Manager --Jean Thierry-Mieg and
   Richard Durbin (1991-) 

Get By anonymous ftp from ncbi.nlm.nih.gov in repository/acedb: 
ftp://ncbi.nlm.nih.gov/respository/acedb/doc*tar.Z and 
ftp://weeds.mgh.harvard.edu/acedb_doc . The files are in TeX (Jean Thierry-Mieg
suggests latex xxx.tex; dvi2ps xxx.dvi > xxx.ps; lpr xxx.ps) and PostScript. 

The Proceedings from the May 1995 ACEDB Conference are available at 
http://probe.nalusda.gov:8000/acedocs/ace95/index.html A final summary report is
available at http://probe.nalusda.gov:8000/acedocs/ace95/ace95.final.html . 

Japanese language guides: _Japanese ACEDB Guide Ver.1.2._ _ACEDB ver.2 for NEC
engineering workstation EWS4800 series_ are available in PostScript via
anonymous ftp at ftp.nec.co.jp as /pub/packages/acedb/acemanjp.1_2.ps.Z . There
is a Japanese language ACEDB FAQ available at 
http://www.cbi.or.jp/~sano/acemanjp12/index.html 

SampleDB, by Dave Matthews, is an ACEDB database constructed to demonstrate
features of ACEDB, especially map and sequence displays. Anonymous ftp to 
probe.nalusda.gov:pub/acedocs/sampledb 

You will find interesting documents in the wdoc subdirectory of the ACEDB
distribution. 

The Australian National Genomic Information Service has prepared good
documentation of the C. elegans version as Angistute.ps and angistute.hqx 
available by anonymous ftp at ncbi.nih.gov in repository/acedb/ace2. 

Cherry, J.M., Cartinhour, S.W., and Goodman, H.M. (1992) AAtDB, An Arabidopsis
thaliana Database. Plant Molecular Biology Reporter 10 (4): 308-309,409-410 

Tutorial manual for AAtDB: Cartinhour, S., Cherry, J.M., and Goodman, H.M.
(1992) An Introduction to ACeDB: For AAtDB, An Arabidopsis thaliana Database.
Massachusetts General Hospital. (Available on request in printed form from the
AAtDB curator). URL : http://genome-www.stanford.edu/docs/aatdb_man.html . 

A description of ACEDB: Cherry, J.M. and Cartinhour, S.W. (1994) ACEDB, A tool
for biological information. in Automated DNA Sequencing and Analysis, edited by
M. Adams, C. Fields, and C. Venter. Academic Press, pages 347-356. [text is
available through ftp or gopher from weeds.mgh.harvard.edu] URL : 
http://probe.nalusda.gov:8000/acedocs/overview.html . 

Another description of ACEDB for physical mapping projects: Dunham, I., Durbin,
R., Mieg, J-T & Bentley, D.R. (1994) Physical mapping projects and ACEDB, in
Guide to Human Genome Computing. Ed. Bishop, M.J. Academic Press, pages 111-158.
[text is available through ftp or gopher from weeds.mgh.harvard.edu] 

Return to List of Questions 


Q6: What other resources are available for ACEDB?
+++++++++++++++++++++++++++++++++++++++++++++++++

A6:
+++

Perl and WWW tools
++++++++++++++++++

The AGIS server at the National Agricultural Library provides a tool set for
integrating version 4 of ACEDB with perl and the World-Wide Web. The archive is 
ftp://probe.nalusda.gov/pub/tools/acelib.tar.gz 

For a general tool for converting data to ACEDB format input files, Joachim
Baumann (joachim.baumann@informatik.uni-stuttgart.de) has written the Perl
program TextConvert, available at ftp.informatic.uni.stuttgart.de/pub/DART/ . 

The Developers' Archive
+++++++++++++++++++++++

Mike Cherry maintains an archive of tools that may be useful in curating ACEDB
databases. It can be reached by anonymous ftp at URL 
ftp://weeds.mgh.harvard.edu/acedb_dev/ or via gopher at 
gopher://genome-gopher.stanford.edu:70/11/ftp 

If you have a contribution place it in
ftp://weeds.mgh.harvard.edu/acedb_dev/incoming/ and send a message to Mike
(cherry@genome.stanford.edu). 

The Biosci conference bionet.software.acedb
+++++++++++++++++++++++++++++++++++++++++++

There is a USENET/Biosci conference titled bionet.software.acedb created
expressly for discussion of ACEDB. The best way to interact with the Biosci
conferences is via a newsreader like rn, trn, tin or a WWW browser. Consult your
system administrator for more information. 

If you do not have access to the Biosci conferences via a newsreader (e.g. rn,
trn, tin) you can participate in the conference by electronic mail. To subscribe
to the e-mail version of the conference send email to biosci-server@net.bio.net
(UK, European readers use biosci@uk.ac.daresbury or biosci.daresbury.ac.uk) with
no subject line and only the message subscribe ACEDB-SOFT in the body. To
unsubscribe send the message unsubscribe ACEDB-SOFT to the same address. This is
an automated service. Your e-mail address will be taken from the header of the
message that you send. If you then send mail to acedb@net.bio.net the mail will
be distributed to all subscribers and to the electronic conference. 

 o All of the articles in biosci.software.acedb are archived by Biosci at 
   gopher://gopher.bio.net/1/ACEDB 
 o Mike Cherry makes it possible to search past articles in
   biosci.software.acedb using WAIS at 
   gopher://genome-gopher.stanford.edu:80/77/.index/acedb-biosci and at URL 
   http://genome-www.stanford.edu/biosci_acedb.html . If your WWW browser is
   configured properly you may be able to read the newsgroup at 
   news:biosci.software.acedb 

. 

And more
++++++++

The AAtDB, Soybase, GrainGenes, Mace, and TreeGenes [see Q4] databases regularly
submit data to the Plant Genome Database at the National Agricultural Library
(NAL). Nal makes this data available via the WWW using an http server with URL: 
http://probe.nalusda.gov:8000/index.html You will also find a selection of
models.wrm files (schemata) for the various databases here. You will want to get
a "mosaic client" to examine this. 

AboutDB is a stab at an integrated info and project tracking database for the
'Greater ACEDB Community'. It was conceived and implemented by Staffan Bergh
(staffan@biochem.kth.se), the 'coordinator', during the ace94 workshop in
Montpellier, based on an earlier effort by John McCarthy. The aim is to collect
information on all aspects of ACEDB use as a database manager. Currently it
contains information on Databases implemented in ACEDB, Colleagues in the
community, some Tools for >curators of ACEDB databases and some of the
information on 'magic tags' collected during the ace94 workshop. AboutDB can be
reached at URL: http://kiev.physchem.kth.se/AboutDB.html 

Other URL's that readers with mosaic clients might want to examine are:

 o http://moulon.inra.fr/acedb/acedb.html for C. elegans data 
 o http://kiev.physchem.kth.se/MycDB.html for Mycobacterium data 
 o http://moulon.inra.fr:8001/acedb/igd.html for an integrated genome database. 

For information on how these were created see 
http://moulon.inra.fr/acedb_conf_eng.html and en francais 
http://moulon.inra.fr/acedb_conf.html A how-to manual on the Moulon server is
available at http://keck.tamu.edu/cgi/staff/ace-mosaic-howto.html 

The Genome Computing Group, Lawrence Berkeley Laboratory, has an anonymous ftp
service at machine genome.lbl.gov which contains: 

 o flydb - LBL's Drosophila Acedb-style database 
 o 21bdb - LBL's Human Chromosome 21 Acedb-style database 
 o querdb - LBL's query-language extensions to Acedb 
 o metadata - LBL's compendium of Acedb database schema variants 
 o macace-aatdb-demo.hqx - pre-release Acedb MacIntosh version 
 o There is also a repository of contributed software for data conversions and
   the like. 

[From Otto Ritter] IGD - the Integrated Genomic Database - is an international
project of DKFZ, Heidelberg (Germany), CNRS, Montpellier (France), ICRF, London
(UK), LBL, Berkeley (USA), and MRC, London/Cambridge, (UK). IGD is an extensible
object-oriented distributed information management system with one global
schema, physical data integration at the back-end, and local data management at
the front-end. It supports local schema evolution and local data integration,
and has a potential for truly virtual "on-the-fly" integration (federation) of
its resource databases. Beside data integration, IGD provides graphical user
interface, client/server communication, and seamless interface to a growing
number of tools for structure, sequence, genetic, physical and comparative
mapping analysis. ACEDB is the IGD main software component for data management.
As a database, IGD integrates and references genome related data from public
sources. As an analysis tool, IGD provides uniform interface to existing
programs and program packages for tructure and sequence analysis, genetic and
physical map construction and analysis, etc. In addition to the major human and
mouse databases already planned SWISS-PROT/PIR, PDB, GDB, OMIM, CitDB, CEPH,
CHLC, CEPH-Genethon, Genbase, UK DNA ProbeBank, RLDB2, CAD, MGD, MouseBackcross
DB), crossreferences will be maintained to dataabases established around
specific model organisms (C.elegans, D. melanogaster, S. cerevisiae, pombe
etc.). Refs: 

 o 1/ Ritter,O.: The Integrated Genomic Database. in Computational Methods in
   Genome Research, edited by S.Suhai, Plenum, 57-73 (1994). 
 o 2/ Ritter,O., Kocab,P., Senger,M., Wolf,D., and Suhai,S.: Prototype
   Implementation of the Integrated Genomic Database, Computers and Biomedical
   Research, 27, 97-115 (1994) 

Computer staff for the UC Berkeley Drosophila physical mapping project the LBL
Human Chromosome 21 project, and the LBL plant genome projects meet regularly to
coordinate their ACEDB extension and development efforts, along with Frank
Eeckman, who is working on the Macintosh version of ACEDB (for further
information, contact jlmccarthy@lbl.gov). They also keep in close touch (via
email, personal visits, etc.) with their counterparts in Cambridge (Richard
Durbin et al), Montpellier Jean Thierry-Mieg et al), and the Interated Genome
Database project in Heidelburg (Otto Ritter, Detlef Wolf et al). 

Return to List of Questions 


Q7: How should ACEDB be cited?
++++++++++++++++++++++++++++++

A7:
+++

From the distribution: 
We realize that we have not yet published any "real" paper on ACEDB. We consider
however that anonymous ftp servers are a form of publication. We would
appreciate if users of ACEDB could quote: 
Richard Durbin and Jean Thierry Mieg (1991-). A C. elegans Database.
Documentation, code and data available from anonymous FTP servers at
lirmm.lirmm.fr, cele.mrc-lmb.cam.ac.uk and ncbi.nlm.nih.gov. 

Papers involved in database development could quote more precisely: 
I. Users' Guide. Included as part of the ACEDB distribution kit, 
II. Installation Guide. Included as part of the ACEDB distribution 
III. Configuration Guide. Included as part of the ACEDB distribution 
and the preprintkit available via anonymous ftp. Jean Thierry-Mieg and Richard
Durbin (1992). Syntactic Definitions for the ACEDB Data Base Manager. Included
as part of the ACEDB distribution. 

--Jean and Richard. 

Return to List of Questions 


Q8: Is ACEDB object-oriented?
+++++++++++++++++++++++++++++

A8:
+++

From the ACEDB User's Guide: 

A major current vogue in computer languages and database design is for
``object-oriented'' systems. It's also a source of lots of argument. We are just
trying to build a good system, and don't want to get caught in the crossfire,
but we do talk about organising our data into objects and classes. We have
undoubtedly been influenced by many of the ideas going around, but it isn't
likely our system would be regarded as kosher by the object- oriented community.
In particular there is no class hierarchy, nor inheritance, and it is written in
a modular but non-ideological way in straight C. However display and disk
storage methods are class dependent. 

In some ways the class hierarchy is replaced by our system of models and trees,
which seems to be rather unusual. We think it is very natural for the
representation of biological information, where for some members of a class a
lot might be known about some aspect, but for most only a little is known. 

The advantages of our sytem over a relational database, such as Oracle or
Sybase, is our ability to refine our descriptions without rebuilding the
database and the possibility of organising the storage of data on disk according
to their class, i.e. we store in a very different way the tree-objects and the
long stretches of DNA sequence. 

Return to List of Questions 


Q9: How can I get on/off the ACEDB announcements mailing list?
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

A9:
+++

To get on or off the mailing list send mail to rd@mrc-lmb.cam.ac.uk or
mieg@kaa.crbm.cnrs-mop.fr. New releases of the software are announced to this
list and very little else. The BIOSCI newsgroup bionet.software.acedb [See Q6
for details] is on the mailing list. 

Return to List of Questions 


Q10: When and where is the Next ACEDB Workshop?
+++++++++++++++++++++++++++++++++++++++++++++++

A10:
++++

The 1996 Workshop is not yet scheduled. 

If you would like to see some pictures of the ACEDB '94 Workshop in St. Matthieu
de Treviers, there are online collections: 

 o by Mike Cherry at
   http://genome-www.stanford.edu/~cherry/pics/acedb-94.pics.html ; 
 o by John Morris at http://weeds.mgh.harvard.edu/ace94/ace94.image.html ; 
 o and by Brad Sherman at ftp://s27w007.pswfs.gov/ACEDB/ace94pix.html 

Return to List of Questions 


Q11:Who prepared this document & where is the current version?
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

A11:
++++

This document will be posted monthly to the BIOSCI newsgroup
bionet.software.acedb and to USENET conference news.answers. It is intended to
be used as an index to ACEDB databases and to information about the database
software. 

The latest text version of the ACEDB FAQ should be available via anonymous ftp
at machine net.bio.net as file pub/BIOSCI/ACEDB/ACEDB.FAQ or at rtfm.mit.edu as 
pub/usenet/news.answers/acedb-faq . If you only have electronic mail, the FAQ
can be retrieved from mail-server@rtfm.mit.edu. 

There is an HyperText Markup Language (HTML) version of this document available
on the World Wide Web: http://probe.nalusda.gov:8000/acedocs/acedbfaq.html and 
http://s27w007.pswfs.gov/Homepage/acedbfaq.html There is a Japanese language FAQ
available at http://www.cbi.or.jp/~sano/acemanjp12/index.html 

Curators of ACEDB databases should take note of Question 4 and keep me apprised
of changes. 

Errors of commission or omission are unintentional. If I have forgotten to give
you credit please let me know. Please send comments and corrections to: 
acedbfaq@s27w007.pswfs.gov 

Major contributions in getting this FAQ off the ground were made by Mike Cherry,
John McCarthy, and Doug Bigwood. Other contributors include: 

 o Lisa Lorenzen 
 o David Matthews 
 o Edie Paul 
 o Donn Davy 
 o Eric De Mund 
 o Sam Cartinhour 

Please cite as: 
Sherman,B.K., ACEDB Genome Database Software FAQ,
ftp://rtfm.mit.edu/pub/usenet/news.answers/acedb-faq,
http://probe.nalusda.gov:8000/acedocs/acedbfaq.html, 1993,1994,1995 approx. 50K
bytes. 

To add or modify information in this document, please send mail to:
acedbfaq@s27w007.pswfs.gov 

Bradley K. Sherman 
Dendrome Project 
Institute of Forest Genetics 
P.O. Box 245, Berkeley, CA, 94701 
Phone: 510-559-6437 Fax: 510-559-6440 

The Dendrome Project and TreeGenes are funded by the USDA ARS Plant Genome
Research Program. 

Return to List of Questions 

End of ACEDB FAQ --bks 


From owner-acedb@net.bio.net Tue Feb 06 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!wizard.pn.com!brighton.openmarket.com!decwrl!tribune.usask.ca!canopus.cc.umanitoba.ca!news
From: Brian Fristensky <frist>
Newsgroups: bionet.software.acedb
Subject: Re: ACEDB3 -> ACEDB4.1
Date: 7 Feb 1996 18:46:36 GMT
Organization: University of Manitoba
Lines: 34
Message-ID: <4fas2c$768@canopus.cc.umanitoba.ca>
References: <4etv4t$pl5@canopus.cc.umanitoba.ca> <4f4b2e$7t8@sibirien.physchem.kth.se>
NNTP-Posting-Host: vogt.cc.umanitoba.ca
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 1.12 (X11; I; SunOS 5.4 sun4m)
X-URL: news:4f4b2e$7t8@sibirien.physchem.kth.se

staffan@biochem.kth.se (Staffan Bergh) wrote:
..deleted
>In article <4etv4t$pl5@canopus.cc.umanitoba.ca> Brian Fristensky <frist> writes:
>
>>I am trying to upgrade from ACEDB3 to ACEDB4.1, but when I start
>>ACEDB4.1, I get errors regarding my models. These models worked
>>in ACEDB3. The errors seem to fall into 3 classes:
.. error descriptions deleted...
>ACEDB4.1 is much more picky. It now does error checking on the models ... Came
>as a shock to me also when moving MycDB from 3 to 4!
>
Yes. All of my problems stemmed from doing things in 3.0 that 4.1 
wouldn't let me get away with. After a bit of tedium, I now have
tighter models and a database that works under 4.1.

>Good Luck /staffan
>
>Staffan Bergh
>Biochemistry, KTH, S-100 44 Stockholm, Sweden
>

Many thanks to those who responded to my original post.

===============================================================================
Brian Fristensky                |  We've heard 3 or 4 times this morning that,
Department of Plant Science     |  out of 85,000 works funded by the NEA,
University of Manitoba          |  only 20 were controversial. I don't know
Winnipeg, MB R3T 2N2  CANADA    |  why anyone would cite that as something to
frist@cc.umanitoba.ca           |  be proud of.
Office phone:   204-474-6085    |  
FAX:            204-261-5732    |  Garrison Keillor, testimony to Congress in
http://home.cc.umanitoba.ca/~frist/  defense of Natl. Endowment for the Arts
===============================================================================


From owner-acedb@net.bio.net Wed Feb 07 22:00:00 1996
Path: biosci!daresbury!not-for-mail
From: mieg@kaa.crbm.cnrs-mop.fr (Danielle et Jean Thierry-Mieg)
Newsgroups: bionet.software.acedb
Subject: Re:  converting to .ace format
Date: 8 Feb 1996 11:50:47 -0000
Lines: 28
Sender: lpddist@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <4fco2o$f2i@mserv1.dl.ac.uk>
Original-To: carson@abacus.mc.duke.edu

acedb 4.2 now reads fasta format
4.1 does not i think (i can t remember if it does in fact)


fasta format is a file called

mydna.ace (other wise the file chooser won t prompt for it)

>ZC23A  junk that will be kept as a remark
atgc
aggttdtggttc
atgcATGCTGATGC
TGA
TTGGTGG

ace format differs by the title line:

DNA ZC23A
atgc
aggttdtggttc
atgcATGCTGATGC
TGA
TTGGTGG

that is it: read the file
there will be a new sequence called ZC23A
pick it and run genefinder
you can then exports the translation to blast it etc

From owner-acedb@net.bio.net Mon Feb 12 22:00:00 1996
Path: biosci!GREENGENES.CIT.CORNELL.EDU!matthews
From: matthews@GREENGENES.CIT.CORNELL.EDU ("Dave Matthews")
Newsgroups: bionet.software.acedb
Subject: escaping "//" in acediff
Date: 13 Feb 1996 12:35:23 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 9
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9602132031.AA24048@greengenes.cit.cornell.edu>
NNTP-Posting-Host: net.bio.net

The Configuration Guide says that in .ace files, "Two consecutive forward
slashes (//) signify the start of a comment. Any text following them is
ignored. You can escape one of them with a backslash if you really want two
forward slashes."

This works for xace, but acediff seems to ignore everything after "\//" or
"\/\/" too.  Is there any way to escape the // in acediff?

- Dave

From owner-acedb@net.bio.net Mon Feb 12 22:00:00 1996
Path: biosci!daresbury!not-for-mail
From: mieg@kaa.crbm.cnrs-mop.fr (Danielle et Jean Thierry-Mieg)
Newsgroups: bionet.software.acedb
Subject: bug in acediff
Date: 13 Feb 1996 23:40:32 -0000
Lines: 4
Sender: lpddist@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <4fr7hg$v3@mserv1.dl.ac.uk>
Original-To: net@admcnrs.cnrs-mop.fr


Dave says: acediff does not escape \/\/
this is an ovious bug
we ll try to patch it soon, sorry

From owner-acedb@net.bio.net Thu Feb 15 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.tamu.edu!algodon!lazo
From: lazo@sun.com (Gerard R. Lazo)
Newsgroups: bionet.software.acedb
Subject: Re: escaping "//" in acediff
Date: 16 Feb 1996 14:33:24 GMT
Organization: Sun Microsystems, Inc.
Lines: 16
Distribution: world
Message-ID: <4g24jk$a6j@news.tamu.edu>
References: <9602132031.AA24048@greengenes.cit.cornell.edu>
Reply-To: lazo@sun.com
NNTP-Posting-Host: algodon.tamu.edu

I tried using "//" in the datainfo.wrm file to highlight a web site.
Everthing beyond "//" was taken as comments/

I tried "\/\/" also, but "\/\/" was printed.

I settled on "/ /", but you need to know that the space shouldn't be 
there.

---
Gerard R Lazo      .-.-.     USDA-ARS Southern Crops Research Laboratory
-. .-.   .-. .-.  .-. ).).   .-. .-.   .-. .-.   .-. .-.   .-. .-.   .-.
||X|||\ /|||X|||\(.( .) .)\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||
|/ \|||X|||/ \|||X(_( ._)||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/
'   `-' `-'   `-' `-//\)`-' `-'   `-' `-'   `-' `-'   `-' `-'   `-' `-'
lazo@tamu.edu      ((        2765 F&B Rd, College Station, TX 77845-9592


From owner-acedb@net.bio.net Thu Feb 22 22:00:00 1996
Path: biosci!daresbury!not-for-mail
From: rd@mrc-lmb.cam.ac.uk (Richard Durbin)
Newsgroups: bionet.software.acedb
Subject: ACEDB update for C.elegans 4-14
Date: 23 Feb 1996 15:06:07 -0000
Lines: 86
Sender: lpddist@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <4gkl4v$f1d@mserv1.dl.ac.uk>
Original-To: acedb_distrib@ppsw.cam.ac.uk


This is a broadcast message to the ACEDB mailing list and related
newsgroups.  If you are on the explicit mailing list and do not want
to be, please send email to rd@sanger.ac.uk.

ACEDB data update 4-14 for C. elegans
=====================================

The additions since 4-13 are:
  - more genomic sequence data (there are now 986 cosmids totalling
      29,930,126 bases).
  - electronic entries in WBG 14 Number 2 have been added
  - a physical map update (January 24)
  - some medline references have been added together with connections
     to sequences from these and cgc_references
  - years have been added to wbg citations
  - new data submitted by email to the CGC in Cambridge or direct to 
	Sylvia has been entered
  - a number of errors and problems have been fixed
  - names of proteins and motifs have been made more systematic, removing
	some duplications

The total database size after adding updates 4-1 to 4-14 is
approximately 260 Mbytes.  The last 10% of the update, where the
protein naming is fixed, is unfortunately extremely slow to read in.
Also at this point the update window will flicker.

Instructions for obtaining updates/the whole thing
==================================================

All the files are available in the following public access accounts
(anonymous ftp sites) accessible over internet:

  ncbi.nlm.nih.gov (130.14.20.1) in the USA, in repository/acedb
  ftp.sanger.ac.uk in England, in pub/acedb
  lirmm.lirmm.fr (193.49.104.10) in France, in directory genome/acedb

In each case, log in as user "anonymous" and give a user identifier
as password.  Remember to transfer the files in BINARY mode by
typing the word "binary" at the start of your ftp session.  Many
thanks to NCBI for letting us share in their excellent resource.

Example:

ftp ncbi.nlm.nih.gov
login: anonymous
password: your user id or email address
cd repository/acedb             # change to relevant directoy
binary				# IMPORTANT
dir				# display files in this directory
get README
get NOTES
get INSTALL
cd ace4				# change to ace4 directory
get bin.sunos.4_1.tar.Z		# get program
cd ../celegans			# change to worm data directory
mget update*			# get all update files
quit

--------------------------------

Get any update.4-n.tar.Z that you do not have already and read the
file NOTES before proceeding further.

Always get a copy of the INSTALL script.  Move it and the .tar.Z files
into the home directory in which you are installing ACEDB.  Type
"source INSTALL".  Start acedb (normally by typing "acedb"), click
"Yes" to accept initialising the database if starting from scratch,
then choose "Add Update File" from the menu (right button), and press
"All updates" with the left mouse button.

If you have a problem making the program work, look at the section
on problems in NOTES, and if that fails to help, let us know.

******************************************************************

Comments about the data should be sent to the data curator, Sylvia
Martinelli (sylvia@sanger.ac.uk).

Comments about the program, or the installation procedure, should be
sent to one of us:

Richard Durbin (rd@sanger.ac.uk)
Jean Thierry-Mieg (mieg@kaa.cnrs-mop.fr)

-------------------- end of message --------------------

From owner-acedb@net.bio.net Fri Feb 23 22:00:00 1996
Path: biosci!daresbury!not-for-mail
From: rd@mrc-lmb.cam.ac.uk (Richard Durbin)
Newsgroups: bionet.software.acedb
Subject: possible ACEDB update problem
Date: 24 Feb 1996 02:42:15 -0000
Lines: 21
Sender: lpddist@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <4gltu7$7hc@mserv1.dl.ac.uk>
Original-To: acedb_distrib@ppsw.cam.ac.uk


I have had a reliable report of a crash while loading the update 4-14
announced yesterday, using the standard 4_1 software on a Sun
computer.  This has not happened with previous updates, or on other
computers of other types that we know load this update OK.  We don't
know yet if the problem is with the specific environment where it
crashed, or is more general.

This type of crash generates the message "segmentation fault" in the
Unix shell window from which you started acedb.  If there is a message
"malloc failure..." then you have run out of memory and should add
swap space using mkfile and swapon (not hard, see your system manager
or man pages for help if necessary).

We will look for a fix/workaround for the segmentation fault problem,
but it may take several days (I will be away for 4 days myself from
now).  If this crash happens to you please email Jean Thierry-Mieg
(mieg@kaa.cnrs-mop.fr) and myself (rd@sanger.ac.uk).  That way you
will be notified about the fix.

Richard Durbin

From owner-acedb@net.bio.net Mon Feb 26 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!ddsw1!news.mcs.net!van-bc!unixg.ubc.ca!news.bc.net!news.sfu.ca!trog.mbb.sfu.ca!jbryer
From: jbryer@trog.mbb.sfu.ca (Jeff Bryer)
Newsgroups: bionet.software.acedb,bionet.celegans
Subject: ANNOUNCE: Linux binary for ACEDB v4.2
Date: 27 Feb 1996 02:32:39 GMT
Organization: IMBB, Simon Fraser University
Lines: 51
Distribution: world
Message-ID: <4gtqg7$f20@morgoth.sfu.ca>
NNTP-Posting-Host: trog.mbb.sfu.ca
Xref: biosci bionet.software.acedb:851 bionet.celegans:822

I have made available both an a.out and an ELF version of 
ACEDB v4.2 for Linux on trog.mbb.sfu.ca in /pub/acedb
(the normal location).  I assume that the files will
migrate over to the ncbi site shortly.  These files
are based on the February 14th release of the 4.2 code.

For the C. elegans community I will be making available a mult-part
archive containing all data releases up to and including 4-14 (same
location).

The readme's follow:

Jeff
jbryer@darwin.mbb.sfu.ca

-------------

README.LINUX.4_2
February 26, 1996

This is the a.out binary version of ACEDB v4.2 for Linux and can be
found at
  ftp://trog.mbb.sfu.ca/pub/acedb/bin.linux.4_2.tar.gz
and soon at
  ftp://ncbi.nlm.nih.gov/repository/acedb/ace4/bin.linux.4_2.tar.gz

If your Linux system cannot handle the a.out format, an ELF version
is also available.
  ftp://trob.mbb.sfu.ca/pub/acedb/bin.linux.4_2.elf.tar.gz

Linux is a free Unix-like OS available for the Intel [3456]86 platform.  
You can find out more about Linux in the comp.os.linux.* groups on usenet.

Acedb runs very well on Linux, but you will need a fairly loaded system.
I reccomend at _least_ 16MB of RAM and a 486/33.  You will also want a
large disk that can accomodate the database's growth.  More RAM is 
much better than a faster processor.  A 486/66 with 32MB RAM will
blow the doors off a Pentium/100 with 8M.  You should have 32MB of swap
for updates.

You should be running Linux 1.0 or better and either the a.out
shared libraries (I am using libc 4.5.26) or the ELF libraries
(I am using libc 5.0.9).  Other versions should work, but are untested.

   Jeff Bryer
   jbryer@darwin.mbb.sfu.ca
-- 
-------
Jeff Bryer               | "Maybe VR.12 turns you into Fabio."
jbryer@darwin.mbb.sfu.ca |   -Duncan in Parallel Lives (VR.5)


From owner-acedb@net.bio.net Tue Feb 27 22:00:00 1996
Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!news.cis.okstate.edu!news.ksu.ksu.edu!pstpm.pp.ksu.edu!user
From: PST@KSU.KSU.Edu (Paul St. Amand)
Newsgroups: bionet.software.acedb
Subject: MacACE 4.1b1 and map display problem
Date: 28 Feb 1996 21:52:24 GMT
Organization: USDA/ARS and KSU Agronomy Dept.
Lines: 13
Message-ID: <PST-2802961552230001@pstpm.pp.ksu.edu>
NNTP-Posting-Host: pstpm.pp.ksu.edu
X-Newsreader: Yet Another NewsWatcher 2.1.7

We are using MacACE 4.1b1 and can not display maps at all. If a map name
is double-clicked, a window opens and then the machine crashes. We had no
trouble with version 2. We have tried numerous models all with the same
result. Any suggestions?

Thanks,

Paul C. St. Amand    (PST@KSU.KSU.Edu)
USDA Research Geneticist        // \\  // \\        // \\  // \\          // 
2004 Throckmorton Hall, KSU  // | :,\\': | \\     // | :,\\': | \\      // | 
Manhattan KS 66506-5501\\  // | | //  \\ | |\\  // | | //  \\ | | \\  // | | 
(913) 532-6168    \\ | :,\\': | //      \\ |:,\\': | //      \\ | :,\\': | //
Fax (913) 532-6167  \\ //  \\ //          \\//  \\ //          \\ //  \\ //  

From owner-acedb@net.bio.net Wed Feb 28 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!news.uiowa.edu!news.physics.uiowa.edu!newsrelay.iastate.edu!news.iastate.edu!macgrant.agron.iastate.edu!user
From: dgrant@iastate.edu (David Grant)
Newsgroups: bionet.software.acedb
Subject: images in MacACE
Date: 27 Feb 1996 21:30:40 GMT
Organization: Iowa State University
Lines: 105
Message-ID: <dgrant-2702961530460001@macgrant.agron.iastate.edu>
NNTP-Posting-Host: macgrant.agron.iastate.edu

Colleagues,

A while back I posted a message asking how to get MacACE to
display images the way the UNIX versions of ACeDB would. No
one had an answer then but after some poking around I have
found a method that works. 

The biggest problem I had was that although the images
distributed with MacACeDB 4-1b1 are labeled as JPEGs, they
are actually PICTs. Only PICTs will work with MacACE. I
discovered this for myself but later found that the images
distributed with MacAAtDB_3-6 are correctly labeled as
PICT.

The PICT images must be in a folder named 'pictures' in the
same folder as MacACE, and the database and wspec folders.
This is in contrast to our UNIX ACeDB where the GIF files
were stored in a folder named 'externalFiles'. 

I altered our models.wrm and options.wrm to change 'Image' 
to 'Picture' to match MacACeDB. Later I found that
'Image' is used in MacAAtDB so the exact name is apparently
not important. The 'Picture' line in my options.wrm was
lifted verbatum from the MacACeDB file. The equivalent line in
MacAAtDB is somewhat different though I don't know the
significance of the differences. All of our *.ace files that
used the tag 'Image' were modified to use 'Picture'. I left
in the 'Pick_me_to_call' line although I am fairly certain
it is not needed.

The following shows the before and after versions of my
*.wrm files. 

original SoyBase UNIX version

in options.wrm
_VImage    -H

in displays.wrm
_DDtImage -g TEXT_FULL_SCROLL -t "Image" -width .6 -height
.4 -help Image

in models.wrm
?Image   Probe ?Probe XREF Image
         Locus ?Locus XREF Image
         Pathology ?Pathology XREF Image
         Gene ?Gene XREF Image
         Pick_me_to_call  UNIQUE Text Text
         ...
         ...
         ...
         Last_update UNIQUE Text

?Gene   Name   Gene_class UNIQUE ?Gene_Class XREF Gene
               Correct_name ?Gene XREF Other_name
               Other_name ?Gene XREF Other_name
        Image ?Image XREF Gene
        ...
        ...
        ...
        Last_update UNIQUE Text


Macintosh SoyBase version

in options.wrm
_VImage     -H
_VPicture   -H -D DtImage  (this line added)

in displays.wrm (unchanged from UNIX original)
_DDtImage -g TEXT_FULL_SCROLL -t "Image" -width .6 -height
.4 -help Image

in models.wrm (all instances of 'Image' in the other classes
               also changed to 'Picture')
?Picture   Probe ?Probe XREF Picture
           Locus ?Locus XREF Picture
           Pathology ?Pathology XREF Picture
           Gene ?Gene XREF Picture
           Pick_me_to_call  UNIQUE Text Text
           ...
           ...
           ...
           Last_update UNIQUE Text

?Gene   Name   Gene_class UNIQUE ?Gene_Class XREF Gene
               Correct_name ?Gene XREF Other_name
               Other_name ?Gene XREF Other_name
        Picture ?Picture XREF Gene
        ...
        ...
        ...
        Last_update UNIQUE Text

I make no claims to being a MacACE expert and it is possible 
that some of the changes I made are unneeded. I would be glad 
to hear from anyone who understands ACeDB better than I as 
to what the changes I made are doing and which, if any, 
should be reversed or modified.

David Grant
dgrant@iastate.edu

-- 
David Grant   dgrant@iastate.edu    (515) 294-1205

From owner-acedb@net.bio.net Wed Feb 28 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!gatech!newsxfer2.itd.umich.edu!chi-news.cic.net!news.uiowa.edu!news.physics.uiowa.edu!newsrelay.iastate.edu!news.iastate.edu!macgrant.agron.iastate.edu!user
From: dgrant@iastate.edu (David Grant)
Newsgroups: bionet.software.acedb
Subject: Re: MacACE 4.1b1 and map display problem
Date: 29 Feb 1996 17:33:25 GMT
Organization: Iowa State University
Lines: 15
Message-ID: <dgrant-2902961133480001@macgrant.agron.iastate.edu>
References: <PST-2802961552230001@pstpm.pp.ksu.edu>
NNTP-Posting-Host: macgrant.agron.iastate.edu

In article <PST-2802961552230001@pstpm.pp.ksu.edu>, PST@KSU.KSU.Edu (Paul
St. Amand) wrote:

> We are using MacACE 4.1b1 and can not display maps at all. If a map name
> is double-clicked, a window opens and then the machine crashes. We had no
> trouble with version 2. We have tried numerous models all with the same
> result. Any suggestions?
> 

I had the same problem. Reverting to 4.0 allowed map displays. I don't
remember where I got this version but I'll send it to you if you can't
find it.

-- 
David Grant   dgrant@iastate.edu    (515) 294-1205

From owner-acedb@net.bio.net Thu Feb 29 22:00:00 1996
Path: biosci!ns1.faseb.org!lamarck.sura.net!newsfeed.internetmci.com!btnet!zetnet.co.uk!usenet
From: chris.ctran@zetnet.co.uk (Chris Tranter)
Newsgroups: bionet.software.acedb
Subject: Internet timer
Date: Fri, 01 Mar 1996 08:05:06 GMT
Lines: 7
Message-ID: <4h5eml$kop@irk.zetnet.co.uk>
NNTP-Posting-Host: man-b2.zetnet.co.uk
X-Newsreader: Forte Free Agent 1.0.82

Internet/modem useage logger and timer program now available!

Please E-mail for download info!

CTran



From owner-acedb@net.bio.net Thu Feb 29 22:00:00 1996
Path: biosci!internet!biosci!not-for-mail
From: biohelp (BIOSCI Administrator)
Newsgroups: bionet.software.acedb
Subject: IMPORTANT - BIOSCI Fundraising Update!
Date: 1 Mar 1996 02:01:03 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 149
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199603011000.CAA02079@net.bio.net>
NNTP-Posting-Host: net.bio.net


I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to
bring you up to date on BIOSCI fundraising progress, a topic of
concern to your future use of this resource.  Thank you in advance for
taking the time to read this message carefully.

Last year we announced that BIOSCI was going to adopt the U.S. Public
Broadcasting System model to fund its operations after our DOE/NSF
grant runs out later this year.  Unlike PBS, we are not soliciting
contributions from users; we are only selling ads on our Web pages
solely to cover our operating costs.  Our goal is to seek sponsorships
until we build up an operating reserve of about $100,000 and then
cease further promotions until we need to build the reserve back up.
(The accountants among our readership will be familiar with the
problem of deferred revenue which we can not safely utilize until ads
have been displayed for a period of time.)  We have three sponsors to
date with a couple more pending.  The process is time-consuming,
however, and we need your help as explained further below.

Our operating costs consist of our network connection, phone lines,
hardware maintenance (we hope to have new and faster hardware soon!),
plus 0.7 FTE of salaries covering UNIX systems admin, technical
support, quality assurance, i.e., testing, of our system, and
administrative costs (such as the time it takes to actually
find/write/call potential sponsors and raise money!).  Although the
BIOSCI staff does get compensated for a portion of the work that they
do, this project has always received a lot of free after-hours and
"vacation" time labor, so we hope that no one will begrudge the time
that we do charge to the project to serve you.  All of the three
part-time staff members, Dave Mack, Julie Lawrence, and myself, have
full time day jobs and families in addition to working hard to keep
this service running for all of you.  Julie and Dave Mack are
subcontractors for BIOSCI; my time that is charged to the project
defrays a portion of my regular salary instead of adding to my income.

Besides having to relocate the project, we were very busy this last
year building new infrastructure such as our WWW hypermail interface
to the system.  This was released last December along with scores of
WAIS indices for the newsgroups.  Virtually everything is complete,
although we do continue to find and fix bugs (many through your
helpful feedback!).  We are still having some problems with our WAIS
indexing.  The archives continue to grow rapidly.  We are running over
100 indexes now versus three previously and any systems crashes cause
greater havoc with the indexing than before!  We are still working to
fix this as fast as our resources permit and appreciate your patience,
but we have been able to automate a lot of the infrastructure to
reduce labor as compared to past requirements.

We have also implemented new software to make moderation of
BIOSCI/bionet newsgroups much easier and combat the growing problem of
Internet junk mail and USENET "spamming."  About 20% of our groups are
now moderated, many of them by the BIOSCI staff!  This, for example,
made a major difference last year in the quality of content in our
EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial
concerns and recruiting firms are using **without charge** to recruit
candidates for positions in the biological sciences.

We are also now in a position to have sponsors for individual
newsgroups as you will have noticed if you have visited
http://www.bio.net/ and clicked on "Access the BIOSCI/bionet
newsgroups" recently.

So, how can you help??
----------------------

As noted above it can take a lot of time to contact potential sponsors
if I have to do it all myself.  Our request is quite simple.  You can
do two important things which will take very little time for you
individually.  

First, please use our WWW system at http://www.bio.net/ to access the
archives.  You can now post or reply to messages via your Web browser.
Your usage helps attract sponsors.  If you contact any of our
sponsors, please be sure to thank them for supporting BIOSCI.  It is
critical for them to get this feedback if they are to continue their
sponsorship for the long term.

Second, if you work for a company or organization that provides
products or services of interest to the biology community, please pass
this message on to your marketing or marketing communications
department or other appropriate group.  Please ask them to help
support BIOSCI by sponsoring our Web site and explain the uses and
benefits of the system to the biology community.  If they are
interested, they can then contact us for further information at our
tech support address, biosci-help@net.bio.net.

Our hope is to quickly raise several large corporate/institutional
sponsors on our heavily-used WWW locations (some stats appended
below), and then end this sponsorship campaign so that our resources
can continue to be used for service provision, not fundraising.  Many
of our specialty newsgroup WWW archives are still used by small
communities of scientists (and they haven't been heavily promoted
yet).  While these may be valuable niche markets to some advertisers,
it will generate more labor and overhead having to find these
sponsors, fairly price the locations, and deal with lots of smaller
sponsorships than fewer mid-to large sponsors.  We are striving to
keep our operation as lean and efficient as possible since we are not
trying to make careers out of running BIOSCI.  We are trying if at all
possible to avoid the administrative overhead entailed with processing
lots of small payments to reach our fundraising goals.

I'd like to thank all of you for your help in advance. In helping us,
you are also helping yourselves, not only in keeping this resource
available for all of the both large and small research communities
that we serve, but also by alleviating the need for us to go back and
compete with researchers for tight grant dollars!  We promised NSF
when we were awarded the BIOSCI grant that we would carry out this
mission to make the service self-supporting.  With your help, we will
succeed in continuing BIOSCI's work into its second decade.  Thank you
very much!

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				biosci-help@net.bio.net


A list of our prime WWW sponsorship locations follow.  Statistics are
for the four week period from 22 Jan. - 18 Feb. 1996 and usage
continues to grow.
----------------------------------------------------------------------

The overall BIOSCI WWW pages are currently visited by users from close
to 5000 unique computer hosts per week.  Web servers only log the
Internet computer/host name and frequently more than one individual
can connect to us from a particular host.

Main home page, http://www.bio.net, visited recently by about 2100
unique hosts per week

Main Newsgroups archives page, http://www.bio.net/archives.html,
visited recently by about 1200 Unique hosts per week

BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html,
visited recently by about 1000 unique hosts per week.

EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ 
and monthly header pages, visited recently by about 600 unique hosts
per week.

Address database search page, http://www.bio.net/addrsearch.html,
visited recently by about 450 unique hosts per week.

Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS-
REAGNTS/ and monthly header pages, visited recently by about 350
unique hosts per week.
----------------------------------------------------------------------

