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To: bionet-software-acedb@cs.washington.edu
From: Carson Thoreen <Carson_Thoreen@brown.edu>
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Subject: Chromosome band objects...
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Hello,
I'm configuring my database to display clone-FISH data on a human 
cytogenetic map using the Chrom_Band and Map objects. Has anyone already 
entered all of the band->Map_position information and would you mind 
emailing me an ace file describing those objects?

Thanks very much,
Carson

Carson_Thoreen@brown.edu





From owner-acedb@hgmp.mrc.ac.uk  Mon Jun  5 09:10:08 2000
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From: zgudmunt@my-deja.com
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Subject: Re: Chromosome band objects...
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	I have all the bands listed in Weizmann's UDB (see examply below) list
of chromosome bands in my Ace. I just parsed the tables that they supply
with my little Perl-script and added (most of) the Dark-tag by hand
afterwards. Do you want to use those band-data? In that case IŽd be
happy to share it with you. I know it's tedious to do by hand, that's
why I wrote  a script to do it :)

                    Mummi, Bioinformatics

Table example:
Band Mb Start Mb End Mb Size
pter 0.000 0.000 0.000
p16.3 0.000 4.231 4.231
p16.2 4.231 7.405 3.174
...etc..

In article <Carson_Thoreen-B6C9F5.15253201062000@news.washington.edu>,
  Carson Thoreen <Carson_Thoreen@brown.edu> wrote:
> Hello,
> I'm configuring my database to display clone-FISH data on a human
> cytogenetic map using the Chrom_Band and Map objects. Has anyone
already
> entered all of the band->Map_position information and would you mind
> emailing me an ace file describing those objects?
>
> Thanks very much,
> Carson
>
> Carson_Thoreen@brown.edu
>
>


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From owner-acedb@hgmp.mrc.ac.uk  Mon Jun  5 18:30:03 2000
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To: bionet-software-acedb@moderators.isc.org
From: Keith Bradnam <keith@thale.nott.ac.uk>
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Subject: problem with peptide display
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Hi,

I've just noticed that the peptide viewer (blixem?) in xace (4.7l)
handles BLASTX and BLASTP homologies but won't handle a TBLASTN homology.
This seems to be due to the view control only allowing a set number of
columns (the view control display box does seem to allow for a 'Homol'
column but I couldn't seem to get this to display anything).

Is there anyway around this or will there be support for TBLASTN
homologies in 4.8???  I can read these homologies in as BLASTX homologies
to get them to display but this would be a little misleading to anybody
using our database. 

Regards,

Keith


~  Keith Bradnam - Developer, Arabidopsis Genome Resource (AGR)
~  Nottingham Arabidopsis Stock Centre - http://nasc.nott.ac.uk/
~  University Park, University of Nottingham, NG7 2RD, UK
~  Tel: (0115) 951 3091 






From owner-acedb@hgmp.mrc.ac.uk  Tue Jun  6 15:55:31 2000
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From: Estann@hotmail.com (Cyril)
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Subject: Re : phredphrap ACE files to ACEDB interface?
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    Hello,

have a look at the CAF package that you will find at the Sanger center.
I have used it to reformat the ACE file from phred/phrap/consed and tigr
assembler as well to the ACE file from ACEDB. It works fine.

Best regards

Florent


---




From owner-acedb@hgmp.mrc.ac.uk  Tue Jun 13 16:41:25 2000
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From: zgudmunt@my-deja.com
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Subject: ACeDB2000 Conference proceedings: news from Vancouver
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	For those who are interested in what is happening this week at the
ACeDB conference/workshop in Vancouver, BC:

	The following URL leads to the official news homepage for the
conference:

	http://www.acedb2000.bcgsc.bc.ca/

	You are welcome to contribute suggestions on topics, schedules and
general conference affairs and in that way participate indirectly in the
conference.


		Mummi


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From owner-acedb@hgmp.mrc.ac.uk  Tue Jun 13 18:50:33 2000
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	For those interested in what's going on at the ACeDB2000 conference
currently in session here in Vancouver BC, the following homepage is the
official news center for daily conference information:

	http://www.acedb2000.bcgsc.bc.ca/

	People  who are interested but unable to attend are encouraged to send
in comments, suggestions or other material and thus contribute to the
conference.


	Gudmundur, appointed conference reporter.


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From owner-acedb@hgmp.mrc.ac.uk  Fri Jun 16 16:20:57 2000
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Just curious?

What are the largest ace databases that people have worked with (in either
size Mb (or even Gb), or number of objects) and have people found any
performance problems with very large databases (obviously this is
dependent on the computer hosting and accessing the database).

Are there any theoretical limits on the number of objects an ace db could
hold?

Keith

~  Keith Bradnam - Developer, Arabidopsis Genome Resource (AGR)
~  Nottingham Arabidopsis Stock Centre - http://nasc.nott.ac.uk/
~  University Park, University of Nottingham, NG7 2RD, UK
~  Tel: (0115) 951 3091 






From owner-acedb@hgmp.mrc.ac.uk  Tue Jun 20 11:24:15 2000
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From: crepinau@sb-roscoff.fr (Crepineau)
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Subject: Re: problem with peptide display
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    Hello,

just in case you did not get any answer,

I think it is in method.ace that you define how homologies are presented
on screen, for example for Blastn this is the definition find in the
distibution of ACeDB :


// data dumped from keyset display

Method : "BLASTN"
Display  Colour LIGHTBLUE
Score_by_width
Score_bounds  100.000000 400.000000
Right_priority  5.200000
Strand_sensitive Show_Up_Strand
Blastn
Blixem_N

Best Regards

Florent


---




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To: bionet-software-acedb@net.bio.net
From: lvandela@bccancer.bc.ca (Louie van de Lagemaat)
Newsgroups: bionet.software.acedb
Subject: Dendrograms in acedb
Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
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Newbie question here

I've been trying to read in a dendrogram in New Hampshire format with
acedb ver 4.8c.  I tried various trees, including the example given in
the help pages.  I got the error:

Improperly formed tree structure or missing semicolon

for every tree I tried to read in.  Any clues as to what might be
wrong?  Thanks.


---




From owner-acedb@hgmp.mrc.ac.uk  Wed Jun 21 09:28:17 2000
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From: crepinau@sb-roscoff.fr (Crepineau)
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Subject: svc_tli_create: could not do t_sync: Illegal file descriptor
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    Hello,

I wrought six month ago about problems with solaris 2.7 and going on the
net. Has anybody managed to find an answer to this problem ? Or should I
wait for the socket ?


jaffar [11]%uname -a
SunOS jaffar 5.7 Generic_106541-04 sun4u sparc SUNW,Ultra-1

jaffar [12]%tail /var/adm/messages
Jun 20 14:27:38 jaffar inetd[183]: /usr/local/bin/rpc.acedb: Hangup
Jun 20 14:27:48 jaffar rpcace[21134]: svc_tli_create: could not do
t_sync: Illegal file descriptor
Jun 20 14:27:48 jaffar rpcace[21134]: cannot create tcp service.
Jun 20 14:27:48 jaffar inetd[183]: /usr/local/bin/rpc.acedb: Hangup
Jun 20 14:27:58 jaffar rpcace[21140]: svc_tli_create: could not do
t_sync: Illegal file descriptor
Jun 20 14:27:58 jaffar rpcace[21140]: cannot create tcp service.
Jun 20 14:27:58 jaffar inetd[183]: /usr/local/bin/rpc.acedb: Hangup
Jun 20 14:28:08 jaffar rpcace[21145]: svc_tli_create: could not do
t_sync: Illegal file descriptor
Jun 20 14:28:08 jaffar rpcace[21145]: cannot create tcp service.
Jun 20 14:28:08 jaffar inetd[183]: /usr/local/bin/rpc.acedb: Hangup
jaffar [13]%

Thank you for reading my message.

Florent


---




From owner-acedb@hgmp.mrc.ac.uk  Wed Jun 21 10:38:29 2000
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To: bionet-software-acedb@net.bio.net
From: Ed Griffiths <edgrif@sanger.ac.uk>
Newsgroups: bionet.software.acedb
Subject: Re: svc_tli_create: could not do t_sync: Illegal file descriptor
Organization: Sanger Centre
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> I wrought six month ago about problems with solaris 2.7 and going on the
> net. Has anybody managed to find an answer to this problem ? Or should I
> wait for the socket ?

Uuuuggghhh, this just seems to be the same old stuff, sun boxes are the only
boxes we have problems with, every time someone updates their operating system
on a sun the RPC server breaks....sigh... The problems are very hard to debug
because of course we don't have the source code for the sun libraries so 

This is a sorry tale, I have been putting my efforts into making the socket
server work and I would suggest that you try using that.

You can pick up the latest monthly development build from:

	ftp://ftp.sanger.ac.uk/pub/acedb/Sanger-release.2000_05_09.tar.gz

and you can see documentation on how to set the server up at:

	http://www.acedb.org/Development/wdoc/SOCKET_aceserver.html

Send me email if you have problems or need help. I really recommend just
dropping the RPC server and going for the sockets version.

cheers Ed

 ------------------------------------------------------------------------------
| Ed Griffiths, Informatics Group,                                             |
|               The Sanger Centre, Wellcome Trust Genome Campus,               |
|               Hinxton, Cambridge CB10 1SA, UK                                |
|                                                                              |
| email: edgrif@sanger.ac.uk      URL: http://www.sanger.ac.uk/Users/edgrif    |
|   Tel: +44-1223-494780 (switch  +44 1223 834244) Fax: +44 1223 494919        |
 ------------------------------------------------------------------------------





From owner-acedb@hgmp.mrc.ac.uk  Wed Jun 21 10:38:39 2000
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To: bionet-software-acedb@net.bio.net
From: Ed Griffiths <edgrif@sanger.ac.uk>
Newsgroups: bionet.software.acedb
Subject: Re: How big is yours?
Organization: Sanger Centre
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> What are the largest ace databases that people have worked with (in either
> size Mb (or even Gb), or number of objects) and have people found any
> performance problems with very large databases (obviously this is
> dependent on the computer hosting and accessing the database).
> 
> Are there any theoretical limits on the number of objects an ace db could
> hold?

This is, unfortunately, a "how long is a piece of string" like question. The
number of objects you can stuff into an ace database will be dependent on the
size of those objects. I'm sure you can appreciate that the size can be just
about anything (allowing for various bits of house-keeping information that
every object stored will need). If you mean "can I have a really large number of
objects ?", then the answer is yes, you are likely to run out of disk space
before acedb runs out of index space.

In terms of performance, again its a bit tricky to be specific, there are two
pathological extremes:

	- if you have very big objects in your database then they will take a long time
to load and use up lots of memory.

	- if you have lots of tiny objects and want to search through them all without
using acedbs indexes, then each object must be loaded and explored and again
this will lead to poor performance.

Most databases sit in between these two and acedbs indexing allows many queries
to be done without the need to load objects and look through them, thus giving
very good query performance.

In terms of how big a database can get, well this is (as far as we know) only
dependent on the biggest file size that can be supported by a system. The file
position is usually given by a "long" which could mean anything from a 32 to a
64 bit signed integer. This implies that EACH of the database blockN.wrm files
could be huge. In practice operating systems usually only allow file sizes
somewhat smaller than the theoretical maximum allowed by the file position size.
Its unlikely that this is going to present problems at the moment.

hope this helps a bit...Ed

 ------------------------------------------------------------------------------
| Ed Griffiths, Informatics Group,                                             |
|               The Sanger Centre, Wellcome Trust Genome Campus,               |
|               Hinxton, Cambridge CB10 1SA, UK                                |
|                                                                              |
| email: edgrif@sanger.ac.uk      URL: http://www.sanger.ac.uk/Users/edgrif    |
|   Tel: +44-1223-494780 (switch  +44 1223 834244) Fax: +44 1223 494919        |
 ------------------------------------------------------------------------------





From owner-acedb@hgmp.mrc.ac.uk  Thu Jun 22 09:39:57 2000
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To: bionet-software-acedb@net.bio.net, edgrif@sanger.ac.uk
Subject: Re: How big is yours?
Newsgroups: bionet.software.acedb
Date: Thu, 22 Jun 2000 09:39:54 +0100 (BST)
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> What are the largest ace databases that people have worked with (in either
> size Mb (or even Gb), or number of objects) and have people found any
> performance problems with very large databases (obviously this is
> dependent on the computer hosting and accessing the database).
> 
> Are there any theoretical limits on the number of objects an ace db could
> hold?

There are databases of several GB that people use daily.

There is currently a theoretical limit of 2^24 (approx 16,000,000) objects per
class, and 256 main classes (i.e. not counting subclasses).  We are thinking
about ways one day in the future to remove those limits, but in practice they
have never approached being a problem.  You can certainly live with several
million objects on a reasonable computer.  As Ed says, the total disk usage
possible is very large (possibly 1k x 2^32 = 4 TB because blocks are determined
by a 32bit integer now).

Richard





From owner-acedb@hgmp.mrc.ac.uk  Tue Jun 27 13:04:45 2000
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From: zgudmunt@my-deja.com
Newsgroups: bionet.software.acedb
Subject: Re: How big is yours?
Organization: Deja.com - Before you buy.
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References: <Pine.SOL.3.96.1000616100025.14266C-100000@thale> <395087D3.288CE1E8@sanger.ac.uk>
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  Hey, guys, thanks for last time. I have noticed what Ed mentioned
about looking through each of very many objects in a large database
leading  to poor performance. I am mirroring the GDB-database amplimer
objects over to my local Ace, with each amplimer being represented by an
STS-object, with up to 15 aliases each (talk about redundancy!) as a
Text tag. I had a problem at first when I was looking for markers by
aliases if they were not found by name; looking through each of those
100.000+arkers and check their alias was of course mindboggingly slow.
But when I changed the Alias from Text to a XREF to an Alias-object and
queried the Alias-class instead, all was well and fast. It did however
give my over 600.000 Alias objects and increased my dbase size !:)


              Mummi


In article <395087D3.288CE1E8@sanger.ac.uk>,
  Ed Griffiths <edgrif@sanger.ac.uk> wrote:
> > What are the largest ace databases that people have worked with (in
either
> > size Mb (or even Gb), or number of objects) and have people found
any
> > performance problems with very large databases (obviously this is
> > dependent on the computer hosting and accessing the database).
> >
> > Are there any theoretical limits on the number of objects an ace db
could
> > hold?
>
> This is, unfortunately, a "how long is a piece of string" like
question. The
> number of objects you can stuff into an ace database will be dependent
on the
> size of those objects. I'm sure you can appreciate that the size can
be just
> about anything (allowing for various bits of house-keeping information
that
> every object stored will need). If you mean "can I have a really large
number of
> objects ?", then the answer is yes, you are likely to run out of disk
space
> before acedb runs out of index space.
>
> In terms of performance, again its a bit tricky to be specific, there
are two
> pathological extremes:
>
> 	- if you have very big objects in your database then they will take a
long time
> to load and use up lots of memory.
>
> 	- if you have lots of tiny objects and want to search through them
all without
> using acedbs indexes, then each object must be loaded and explored and
again
> this will lead to poor performance.
>
> Most databases sit in between these two and acedbs indexing allows
many queries
> to be done without the need to load objects and look through them,
thus giving
> very good query performance.
>
> In terms of how big a database can get, well this is (as far as we
know) only
> dependent on the biggest file size that can be supported by a system.
The file
> position is usually given by a "long" which could mean anything from a
32 to a
> 64 bit signed integer. This implies that EACH of the database
blockN.wrm files
> could be huge. In practice operating systems usually only allow file
sizes
> somewhat smaller than the theoretical maximum allowed by the file
position size.
> Its unlikely that this is going to present problems at the moment.
>
> hope this helps a bit...Ed
>
>
------------------------------------------------------------------------------
> | Ed Griffiths, Informatics
Group,                                             |
> |               The Sanger Centre, Wellcome Trust Genome
Campus,               |
> |               Hinxton, Cambridge CB10 1SA,
UK                                |
>
|
|
> | email: edgrif@sanger.ac.uk      URL:
http://www.sanger.ac.uk/Users/edgrif    |
> |   Tel: +44-1223-494780 (switch  +44 1223 834244) Fax: +44 1223
494919        |
>
------------------------------------------------------------------------------
>
>


Sent via Deja.com http://www.deja.com/
Before you buy.





From owner-acedb@hgmp.mrc.ac.uk  Thu Jun 29 11:32:36 2000
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To: bionet-software-acedb@net.bio.net
From: crepinau@sb-roscoff.fr ("Florent.Crepineau")
Newsgroups: bionet.software.acedb
Subject: xaceclient ?
Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
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	Hello,

I would like to know what one can do with the xaceclient. Can one connect from a windows box with the xaceclient over the net to a saceserver running on a unix box? How can I do that ?

Thank you for reading my message

Best Regards

Florent


---




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To: bionet-software-acedb@cs.washington.edu
From: Carson Thoreen <Carson_Thoreen@brown.edu>
Newsgroups: bionet.software.acedb
Subject: Constructed types in Ace 5
Organization: University of Washington
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Hello --
I'm using the 5/9 development release and have found a couple problems 
that may or may not be my fault:

1. If I construct a model like this:

?Dog BelongsTo Text
     DogHouse Text #Yard

?Yard Frontyard
      Backyard
      InTheGarden

and then try to import an .ace file like this:

Dog : Rufus
DogHouse "Deluxe" Frontyard

I get an error claiming Frontyard doesn't exist in the model. When I 
create a Dog object through the edit menu and then choose to update it, 
the constructed type shows up as "DogHouse Text #SomeOtherClass"! If I 
open the "Model" class and examine the "Dog" model, I see "DogHouse Text 
#Yard". So I think there's a problem in the way ace is parsing the 
models for creating objects. Ideas?


2. In the last newsletter, there was mention that indexing in ace 5 was 
a little screwy so that queries wouldn't work correctly unless the 
database was re-indexed. I've had this problem with AQL -- the objects 
returned meet the query criteria, but the data from tags isn't right ie. 
in a database with lots of "Dog" objects, "Rufus" might correctly 
returned, but Rufus->BelongsTo column often shows data that isn't 
connected to "Rufus". Even when I choose "read models and reindex" the 
problem remains. Is there another way to re-index, or should I wait 
until the next release?


3. When I try to go to www.acedb2000.bcgsc.bc.ca to see what I've 
missed, my browser can't find the server. Is this the correct URL?

Any help is very much appreciated!
Thanks,
Carson

---------------------
Carson Thoreen
Dept. of Molecular Biotechnology
University of Washington
Carson_Thoreen@brown.edu





From owner-acedb@hgmp.mrc.ac.uk  Thu Jun 29 11:52:22 2000
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As I announced in an earlier append, I have been producing a monthly Newsletter
for acedb users in the Sanger Centre. At Acedb2000 we agreed that I would append
this newsletter to this newsgroup, I will be doing this later today. If you
would also like to receive the letter in text form via email then send me a note
at edgrif@sanger.ac.uk, remember that you can also access all the newsletters
at:
	http://www.acedb.org/winfo/Newsletters/

Currently I write the Newsletter and put in it whatever I think is of interest
to Sanger users. If you would like to contribute a short article about something
you have discovered or find useful in acedb then please send it to me and I will
include it in a future Newsletter. I want the Newsletter to be of general
interest to as many acedb users as possible.

The idea of the Newsletter is to keep users/developers in touch with what's
going on in acedb, i.e. to provide more feedback. I also want it to include
hints/tips/how-to type articles as well.

Comments are welcome, but articles are even more welcome !!

cheers Ed

 ------------------------------------------------------------------------------
| Ed Griffiths, Informatics Group,                                             |
|               The Sanger Centre, Wellcome Trust Genome Campus,               |
|               Hinxton, Cambridge CB10 1SA, UK                                |
|                                                                              |
| email: edgrif@sanger.ac.uk      URL: http://www.sanger.ac.uk/Users/edgrif    |
|   Tel: +44-1223-494780 (switch  +44 1223 834244) Fax: +44 1223 494919        |
 ------------------------------------------------------------------------------





From owner-acedb@hgmp.mrc.ac.uk  Thu Jun 29 13:11:26 2000
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From: matthews@greengenes.cit.cornell.edu (Dave Matthews)
Newsgroups: bionet.software.acedb
Subject: Re:  Constructed types in Ace 5
Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
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X-Cc: ACEDB newsgroup <acedb@net.bio.net>,
	Ed Griffiths <edgrif@sanger.ac.uk>,
	Richard Bruskiewich <rbsk@sanger.ac.uk>,
	matthews@greengenes.cit.cornell.edu
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Hi Carson,

Sorry, that host turned into a pumpkin at the end of the meeting.
The stuff that was there is supposed to go onto www.acedb.org.  I guess
there'll be an announcement when it's online.
- Dave

> From: Carson Thoreen <Carson_Thoreen@brown.edu>
> Newsgroups: bionet.software.acedb
> Date: Thu, 29 Jun 2000 11:32:41 +0100 (BST)
> 
> When I try to go to www.acedb2000.bcgsc.bc.ca to see what I've
> missed, my browser can't find the server. Is this the correct URL?


---




From owner-acedb@hgmp.mrc.ac.uk  Thu Jun 29 14:14:32 2000
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From: rbsk@sanger.ac.uk (Richard Bruskiewich)
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Subject: Re:  Constructed types in Ace 5
Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
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Hi Dave,

As you can imagine, acedb has taken back seat to the working draft and
EnsEMBL for the moment. I'm sure Ed will get some of the www.acedb.org
updates in place once the dust has settled around here and he can engage a
few of the team web to do the magic.

I've had an initial chat with the wiki people here about the docs
service. again, we'll have to wait our turn at the time slicing of web
expertise.

cheers,
richard b.

ps. Mummi has promised us a draft of his edited Acedb '2000 proceedings
notes sometime soon.

On Thu, 29 Jun 2000, Dave Matthews wrote:

> Hi Carson,
> 
> Sorry, that host turned into a pumpkin at the end of the meeting.
> The stuff that was there is supposed to go onto www.acedb.org.  I guess
> there'll be an announcement when it's online.
> - Dave
> 
> > From: Carson Thoreen <Carson_Thoreen@brown.edu>
> > Newsgroups: bionet.software.acedb
> > Date: Thu, 29 Jun 2000 11:32:41 +0100 (BST)
> > 
> > When I try to go to www.acedb2000.bcgsc.bc.ca to see what I've
> > missed, my browser can't find the server. Is this the correct URL?
> 

========================================================
Dr. Richard Bruskiewich		<rbsk@sanger.ac.uk>
	WWW: http://www.sanger.ac.uk/Users/rbsk/
Human Analysis/Bioinformatics
The Sanger Centre		Tel:  +44-(0)1223-834244
Wellcome Trust Genome Campus,	Fax:  +44-(0)1223-494919
Hinxton, Cambridge   CB10 1SA
United Kingdom
========================================================


---




From owner-acedb@hgmp.mrc.ac.uk  Thu Jun 29 14:14:33 2000
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To: bionet-software-acedb@net.bio.net
From: Ed Griffiths <edgrif@sanger.ac.uk>
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Subject: Acedb - June Newsletter
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Below is Junes Acedb Newsletter.

Please note that the newsletter is biased towards Sanger Centre users, I'm happy
to see this emphasis change a but will need articles (of a few paragraphs) from
other people. I can't undertake to do a "world-wide" newsletter on my own !!

cheers Ed

 ------------------------------------------------------------------------------
| Ed Griffiths, Informatics Group,                                             |
|               The Sanger Centre, Wellcome Trust Genome Campus,               |
|               Hinxton, Cambridge CB10 1SA, UK                                |
|                                                                              |
| email: edgrif@sanger.ac.uk      URL: http://www.sanger.ac.uk/Users/edgrif    |
|   Tel: +44-1223-494780 (switch  +44 1223 834244) Fax: +44 1223 494919        |
 ------------------------------------------------------------------------------

----------------------------------------------------------------------------
----------------------------------------------------------------------------

ACEDB User Group Newsletter - June 2000
#######################################

If you want to have this newsletter mailed to you _or_ you want to make
comments/suggestions about the format/content then send an email to
acedb@sanger.ac.uk.

This month saw the first Acedb Workshop since 1997, which took place in
Vancouver. Thanks go to Richard Bruskiewich and Steve Jones (a former Sanger
person, who now works at the Genome Sequence Centre, Vancouver) for their
efforts in organising the workshop. This issue will be about the conference
and what happened there.

General News
************

Apologies
+++++++++

Apologies for no Acedb Users meeting this month, in the end there just was
not time to organise one.

There will be a meeting in July, if you have issues you want to raise before
then, please come and see the acedb developers in our new room (D2-12) which
is just down from reception, near personnel/accounts etc.

The Newsletter
++++++++++++++

Partly as a result of requests at the conference and partly because of
independent requests from acedb users at other sites, this newsletter is now
being added to the acedb newsgroup each month and is also being sent out to
users at other sites. I hope to solicit some useful articles from contacts
made at the conference.

Acedb2000 at Vancouver
**********************

I'm not going to cover everything that happened at Vancouver, the following
sections deal with subjects most relevant to the Sanger Centre. A fuller
description of the conference will appear on the acedb web site in due
course.

Grid
++++

Gudmund Thorission from Iceland gave a short presentation on how he hopes to
use the grid models/display for micro-array data. The main points for
discussion were:

   * scale - can acedb hold the amount of data that micro-arrays would
     produce.
   * colours - is it possible to colour code the squares in the grid
     display so that they look meaningful to the user.
   * intelligent display - can the grid display be made to work to allow
     reasonable scrolling across a micro-array but not need to load in the
     data for the entire array at once (as this may require prohibitive
     amounts of memory).

This conversation mirrors several that have happened in the Sanger Centre
over at least the last year (e.g. with Dan Lawson and Adam Butler). What's
required now is an initial specification which includes:

   * expected size of micro-arrays
   * amount of data per micro-array element
   * some typical colour schemes

Adam Butler is going to take an initial stab at this, but if you are
interested in seeing this done then don't sit back, either start a
discussion on the acedb newsgroup or email the acedb developers direct
(acedb@sanger.ac.uk).

Zacedb
++++++

Richard Durbin spent much of his time at Vancouver working on a new storage
layer for acedb. This is the part of acedb that stores/retrieves objects
to/from disk. The idea here would to allow a choice of "backends", perhaps
including free (e.g. mySQL, BerkeleyDB, the original acedb system) or
commercial (e.g. Oracle, Sysbase) systems to be used. The backend would not
"know" about acedb objects, it would only be used to store/retrieve them and
provide other services such as locking.

The rationale for doing this is that the backend system could be used to
allow truly concurrent reading/writing via threading and more reliability
via support for transactions. This would be a great advance for acedb and
would supply features requested by Sanger and external users.

This work is currently only at the "broken prototype" stage, much work needs
to be done on further prototyping, in particular to assess whether
performance would be good enough with a different backend.

The acedb web site
++++++++++++++++++

The session on the format/usability of the acedb web site produced one main
conclusion: it's not very user friendly. Fortunately there were also a lot
of suggestions for improvements including:

   * completely revamping the downloads section
   * less developer, more user stuff
   * provide search facilities for acedb keywords
   * sort the documentation out !

and a host of other good suggestions.

We plan to substantially reorganise the web site over the next few weeks.

socket server
+++++++++++++

There were a number of talks/tutorials on how to use/set up the new
sockets-based version of the acedb server/client (available now in Acedb
4_8c in ~acedb/RELEASE.DEVELOPMENT/bin.XXX directories). If you would like
more information or a tutorial on the new server/client system (how to set
up user passwords etc.) then contact Ed Griffiths (edgrif@sanger.ac.uk).

Other important developments related to the socket server included:

   * AcePerl - Lincoln Stein has produced a new version of AcePerl that
     now talks directly to the socket server, you can get more information
     on this from: http://stein.cshl.org/AcePerl/. Note that because AcePerl
     can now talk directly to the server you will be able to use AcePerl
     from Windows and Apple Macs because AcePerl is now purely in Perl with
     no C code compilation required.
   * AcePython - Sean Powell & Siegfried Schloissnig wrote a python
     interface to the socket server whilst at the conference. If you want
     access to this code send a note to Ed Griffiths (edgrif@sanger.ac.uk).

Note that you can also interface to the socket server from Java:
(http://www.biojava.org/docs/api/.

SMAP - a new way to construct sequences
+++++++++++++++++++++++++++++++++++++++

There has been a lot of discussion at Sanger over how to construct larger
sequences up to whole chromosome level. There was a whole session devoted to
this with an introductory talk by Richard Bruskiewich, followed by a talk by
Richard Durbin which gave an overview of the proposed model for SMAP and a
model for exons.

A fair summary would probably be that the SMAP model should solve a lot of
the problems that were experienced with chromosome 22 and with large
sequence construction in general. The difficult bit of the code still needs
writing but that should happen over the next few weeks.

What to do about building haplotype sequences remains unsolved however.

AQL
+++

There was a lot of interest in AQL as a more powerful replacement for the
Query language and TableMaker. We would welcome more reports about how
people find AQL to use, any performance problems etc.

A major requirement for AQL is some kind of query builder and also more
tutorial documentation of the "how to..." type. Anyone fancy writing an
article on how to use AQL ??

Acedb and the Mac
+++++++++++++++++

There were a significant number of users who would like to see a more up to
date copy of acedb available on the Mac. Simon Kelley has made some headway
with obtaining a software environment in which to build acedb on the Mac.
The basic problem here is that we do not have the resources to maintain code
that requires a completely different build environment, graphics routines
etc.

Things look hopeful but no more than that.

Next User Group Meeting - D213, 2.30pm, Thursday, 13th July
***********************************************************

----------------------------------------------------------------------------
----------------------------------------------------------------------------
Ed Griffiths <edgrif@sanger.ac.uk>
Last modified: Thu Jun 29 14:03:23 BST 2000





