From owner-acedb@hgmp.mrc.ac.uk  Fri Dec  1 09:22:57 2000
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From: Jean Thierry-Mieg <mieg@ncbi.nlm.nih.gov>
Reply-To: Jean Thierry-Mieg <mieg@ncbi.nlm.nih.gov>
Subject: Re: Update sequence?
To: bionet-software-acedb@moderators.isc.org, JRSchwartz@lbl.gov,
	keith@thale.life.nottingham.ac.uk
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There is a call in the acedb kernel to automatically
update all coordinates in touched objects up and down the hierarchy
provided they are declared withe the modifier COORD in the
ace model

however, this call is not included in any public interface
and my self i never use it
so i cannot garantee the reliability, but i beleive it works

this means that to use this call you would have to write some c
code

if there is demand, you could trigger the interest of the cambridge
group to add that public interface but just now, i could not 
spare the needed time for this, sorry

jean
>To: bionet-software-acedb@moderators.isc.org
>From: Keith Bradnam <keith@thale.life.nottingham.ac.uk>
>Subject: Re: Update sequence?
>Mime-Version: 1.0
>X-Trace: oyez.ccc.nottingham.ac.uk 974805477 13264 128.243.116.150 (21 Nov 2000 
11:17:57 GMT)
>X-Complaints-To: usenet@news.nottingham.ac.uk
>NNTP-Posting-Date: 21 Nov 2000 11:17:57 GMT
>To: JRSchwartz@lbl.gov
>X-Sender: keith@thale
>Date: Tue, 21 Nov 2000 15:58:15 +0000 (GMT)
>
>On 20 Nov 2000 JRSchwartz@lbl.gov wrote:
>
>> 
>> 
>> Hi all,
>> 
>> I am using acedb for annotation.  I was wondering if there is a way to
>> update the dna sequence for a clone, to add more to it, while preserving
>> the existing annotations.  I have done it in the past by hand, changing
>> all the froms and tos, but it is a royal pain.  Is there a nice way to
>> do that?  If I just delete the DNA sequence and add the new one, will
>> the annotation be automatically updated?
>
>DNA sequences are usually specified as 'UNIQUE' objects in most acedb
>databases, so if you try to read a new DNA sequence as part of an existing
>Sequence object it should just replace the existing DNA sequence but not
>change anything else.
>
>E.g. if your sequence/clone object in your database is something like:
>
>
>Sequence : "EMBL:AB006697"
>DNA      "EMBL:AB006697" 80970
>Version  "AB006697.1"
>Database_date    "26-OCT-2000"
>Species  "Arabidopsis thaliana"
>Title    "Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone:MAH20."
>Clone    "MAH20"
>
>
>Then all you should have to do is put your new DNA sequence in an ace file
>with the same name as that which already exists and read this into the
>database.
>
>
>Keith
>
>~  Keith Bradnam - UK CropNet (http://ukcrop.net) webmaster and
>~  Arabidopsis Genome Resource  (http://ukcrop.net/agr) developer.
>~
>~  Nottingham Arabidopsis Stock Centre, University Park,
>~  University of Nottingham, NG7 2RD, UK.  Tel: (0115) 951 3091 
>
>
>
>





From owner-acedb@hgmp.mrc.ac.uk  Fri Dec  1 09:23:01 2000
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From: Jean Thierry-Mieg <mieg@ncbi.nlm.nih.gov>
Reply-To: Jean Thierry-Mieg <mieg@ncbi.nlm.nih.gov>
Subject: Re: non-native byte order
To: jayoung@fhcrc.org
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if you dump and reload, the system will be marginally faster but on the other 
hand this will probably decrease the disk usage.
as you can see, acedb is platform independent!
jean

>To: bionet-software-acedb@net.bio.net
>From: jayoung@fhcrc.org (Janet Young)
>Subject: non-native byte order
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>
>Hi
>
>We've moved labs recently and have moved our acedb database to a new server
>(from OSF1 on an alpha to SunOS 5.7). Now when I start the new database I
>get 
>// Database has non-native byte-order: swapping
>(I tried editing and saving but that's not enough to fix it)
>
>This doesn't seem to be a problem right now but I just wondered if it leaves
>us more open to crashes or if it will make queries slow? How could I swap
>the byte order?
>
>thanks
>
>Janet
>
>-------------------------------------------------------------------
>                              Dr. Janet Young
>Fred Hutchinson Cancer Research Center
>1100 Fairview Avenue N.                    tel: (206) 667 1471
>C3-168                                     fax: (206) 667 6524
>P.O. Box 19024                             email: jayoung@fhcrc.org
>Seattle, WA 98109-1024, USA.
>
>
>
>---
>
>

Danielle Thierry-Mieg
NCBI. NLM. NIH Bldg 38A
8600 Rockville Pike
Bethesda, MD 20894, USA
email   mieg@ncbi.nlm.nih.gov
Tel   1 (301) 594 70 92   Fax   1 (301) 480 92 41





From owner-acedb@hgmp.mrc.ac.uk  Fri Dec  1 13:20:34 2000
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From: Ed Griffiths <edgrif@sanger.ac.uk>
To: Acedb Newsletter <acedbg@sanger.ac.uk>,
	acedb newsgroup on bionet <bionet-software-acedb@net.bio.net>,
	aratnasiri@ucdavis.edu, boberkfe@watson.wustl.edu,
	carson@mbt.washington.edu, dbaillie@gene.mbb.sfu.ca,
	dgrant@iastate.edu, G.REYES@CGIAR.ORG, jayne.vallance@bbsrc.ac.uk,
	jayoung@fhcrc.org, jmh.neefs@pandora.be, jspieth@watson.wustl.edu,
	jurg@sanger.ac.uk, keith@thale.life.nottingham.ac.uk,
	Lincoln Stein <lstein@cshl.org>, lnothake@watson.wustl.edu,
	lvandela@bccancer.bc.ca, matthews@greengenes.cit.cornell.edu,
	mieg@sanger.ac.uk, mummi@decode.is, mwilkinson@gene.pbi.nrc.ca,
	r.bruskiewich@CGIAR.ORG, sjones@bcgsc.bc.ca, sness@bcgsc.bc.ca,
	T.van_Wezel@lumc.nl
Subject: September newsletter
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Please find the below the long delayed September newsletter.

cheers Ed

 ------------------------------------------------------------------------
| Ed Griffiths, Acedb development, Informatics Group,                    |
|               The Sanger Centre, Wellcome Trust Genome Campus,         |
|               Hinxton, Cambridge CB10 1SA, UK                          |
|                                                                        |
| email: edgrif@sanger.ac.uk   URL: http://www.sanger.ac.uk/Users/edgrif |
|   Tel: +44-1223-494780       Fax: +44 1223 494919                      |
 ------------------------------------------------------------------------


----------------------------------------------------------------------------
----------------------------------------------------------------------------

ACEDB User Group Newsletter - September 2000
############################################

If you want to have this newsletter mailed to you _or_ you want to make
comments/suggestions about the format/content then send an email to
acedb@sanger.ac.uk.

Apologies
+++++++++

There have been no newsletters for too long, sorry, sorry, sorry. We have
had a number of problems with the build/test system for acedb which have
precluded the usual monthly releases of development code. This has ended up
with me delaying sending out the newsletters because there seemed little
point when they refer to new features/bug fixes in code that is not yet
available.

General News
************

_Xref bug believed fixed..._ a long standing bug in xref which resulted in
"object locked" errors when trying to update databases with objects
containing particular types of xrefs has now been fixed (see bugs section).

Most news this month is about bug fixes as we get ready for the next
significant release which will be called Acedb 4_9.

New Features
************

tace command sets
+++++++++++++++++

If you issue the "?" command from within tace you get to see a list of the
commands that you can issue. Previously in tace (and aceclient) this list
was misleading because it included commands that the user would not be able
to run because they did not have write access to the database. This has now
been fixed so that if a user does not have write access they will not see
commands that require write access.

Bugs Fixed
**********

tace bug
++++++++

When tace is run via a pipe (e.g. from a perl or shell script) it saves the
database on quitting by default. This behaviour was broken in 4_8 and has
now been restored. This is particularly important because running tace via
scripts is one of the main mechanisms used at Sanger for doing large scale
updates to databases.

Expanding database
++++++++++++++++++

There was a problem in 4_8 with databases growing enormously when a large
number of separate updates were made to the database. This has been fixed
and was caused by old sessions (a session is a record of the state of the
database after a set up updates has been made) not being deleted correctly
from the database.

To see the benefit from this fix you will need to rebuild the database.

CDS start/end positions
+++++++++++++++++++++++

Perhaps not a bug, more a confusion in meaning. For a sequence object that
is a CDS you can specify the start/end positions of the CDS within the
accompanying Source_exons and here in lies the problem. The start/end
positions must be specified in spliced DNA coordinates, _not_ in Source_exon
coordinates. This means you have to do some mental arithmetic if you are
inserting the CDS positions by hand: you will need to add up the length of
the Source_exon sections to get the size of the spliced DNA and work out
where the CDS starts and ends within the single continuous section of exon
DNA.

acedb will now output an error message if a CDS is displayed where the
start/end positions lie outside of the spliced DNA and will display the CDS
as if it occupied the entire length of the spliced DNA.

CDS start/end positions not working for reverse genes
+++++++++++++++++++++++++++++++++++++++++++++++++++++

There was an annoying bug whereby, for reverese genes only, acedb would
ignore a CDS start position unless an end position was specified as well.
This has been fixed so that a CDS for a reverse gene now behaves the same
way as for forward genes.

Obscure socket server bug
+++++++++++++++++++++++++

When started by inetd, the socket server crashed inetd on some linux systems
because it wrongly _"shutdown"_ the listening socket when exitting. This has
been fixed.

XREF problem
++++++++++++

There has been a bug in the XREF code when making updates to a database for
a long time. In 4_8 this has manifested itself in the form of updates
failing where they involve XREFs with UNIQUE tags. This seems now to be
fixed at long last, if this is not so then please send reports to
acedb@sanger.ac.uk.

This is an important bug to fix because it has been the principal reason for
not releasing updates to large databases as "diffs". Currently databases
such as the Worm database have to be released as complete new copies every
time there is an update. Now the Xref bug is fixed it should be possible to
release an update file which consists only of those parts of the database
are different since the previous relase.

No monthly build available.
***************************

Unfortunately, due to system problems at the Sanger Centre a monthly build
has not been possible for September.

Next User Group Meeting - D213, 2.30pm, Thursday, 14th September
****************************************************************

----------------------------------------------------------------------------
----------------------------------------------------------------------------
Ed Griffiths <edgrif@sanger.ac.uk>
Last modified: Thu Nov 30 15:30:54 GMT 2000






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From: Ed Griffiths <edgrif@sanger.ac.uk>
To: Acedb Newsletter <acedbg@sanger.ac.uk>,
	acedb newsgroup on bionet <bionet-software-acedb@net.bio.net>,
	aratnasiri@ucdavis.edu, boberkfe@watson.wustl.edu,
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	jayoung@fhcrc.org, jmh.neefs@pandora.be, jspieth@watson.wustl.edu,
	jurg@sanger.ac.uk, keith@thale.life.nottingham.ac.uk,
	Lincoln Stein <lstein@cshl.org>, lnothake@watson.wustl.edu,
	lvandela@bccancer.bc.ca, matthews@greengenes.cit.cornell.edu,
	mieg@sanger.ac.uk, mummi@decode.is, mwilkinson@gene.pbi.nrc.ca,
	r.bruskiewich@CGIAR.ORG, sjones@bcgsc.bc.ca, sness@bcgsc.bc.ca,
	T.van_Wezel@lumc.nl
Subject: November newsletter
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and the November newsletter....

the next one should be out around mid-December....

 ------------------------------------------------------------------------
| Ed Griffiths, Acedb development, Informatics Group,                    |
|               The Sanger Centre, Wellcome Trust Genome Campus,         |
|               Hinxton, Cambridge CB10 1SA, UK                          |
|                                                                        |
| email: edgrif@sanger.ac.uk   URL: http://www.sanger.ac.uk/Users/edgrif |
|   Tel: +44-1223-494780       Fax: +44 1223 494919                      |
 ------------------------------------------------------------------------


----------------------------------------------------------------------------
----------------------------------------------------------------------------

ACEDB User Group Newsletter - November 2000
###########################################

If you want to have this newsletter mailed to you _or_ you want to make
comments/suggestions about the format/content then send an email to
acedb@sanger.ac.uk.

A new acedb developer joins the team, a number of new features for users and
a number of bug fixes.

General News
************

New acedb developer
+++++++++++++++++++

After a hiatus of some months following the departure of Fred Wobus from our
team (now pursuing his hobby of scuba diving in Thailand !), we have been
joined by John Sturdy (jcgs@sanger.ac.uk). John is a Computer Science Ph.D.
and has worked for various software companies locally for some years.
Initially he will be working on improving the acedb web site so you should
see quite a few improvements there over the next few weeks.

New Features
************

"Tree/Text" view and "update" mode
++++++++++++++++++++++++++++++++++

An irritating feature of using "update" mode in the "Tree" window has been
that the window is repositioned at the top when the mode is selected, thus
losing the users place each time an update is performed. This has now been
fixed: select a tag by clicking on it, then select "update" from the right
button menu and the tag will stay visible in the window.

"Tree/Text" view and "Tag search" menu item
+++++++++++++++++++++++++++++++++++++++++++

Searching for a particular tag in a large object in the tree window can be
tedious, especially when this involves opening collapsed nodes to find the
tag. The right button menu now has an additional "Find tag" item that allows
you to search for a tag (it will find the tag whether the tag is within a
collapsed node or not).

Fmap Horizontal Scrolling
+++++++++++++++++++++++++

A horizontal scroll bar has been added to the fmap display so its no longer
necessary to turn columns off to see whats displayed over to the right of
the display. Headings/Titles/Column buttons are not redrawn as you scroll
because this slows scrolling down too much.

Support for 2-button mouse users
++++++++++++++++++++++++++++++++

Most MS-Windows users have 2-button mice which means they have not been able
to use functions that were linked to the middle button on Xwindows/Unix
machines. This has now been fixed by making shift-leftmousebutton do the
same job as the middle button for 2-button users.

Change to help in tace
++++++++++++++++++++++

The _"?"_ command to get help in tace has changed in the way it works. If
there is only one command matching the string following "?" then the syntax
for that one command will be printed, if there is more than one, then syntax
for all matching commands will be printed, e.g.

"?"
     gives a list of all available commands
"? d"
     will give a list of all command beginning with "d"
"? du"
     will list the "dump" command because it's the only command that matches
     "du"

New "spick" addtion to keyset manipulating commands in tace.

If you use the spush, spop etc. commands for manipulating keysets in tace
then you should note a useful addition to these of spick (courtesy of
Lincoln Stein). spick allows you to choose one of the stacked keysets and
make it the active keyset without having to spop the stack to get to the one
you want.

Bugs Fixed
**********

Two Acedb MS-Windows bug fixes
++++++++++++++++++++++++++++++

Printing acedb windows now works from Windows 95 in the same way as for
acedb on Unix machines.

You can now use the up and down arrows to scroll through the items in the
keyset window.

xace/tace hang when trying to get write access
++++++++++++++++++++++++++++++++++++++++++++++

Oh dear, while I'd love to say this bug was fixed, its not and can't be for
the foreseeable future. The cause of the bug is based in NFS/lockmanager
problems which arise from time to time on the Sanger network. Acedb uses
lock files to make sure that only one user can have write access to a
database at a time, this works well most of the time. There can be problems
in two situations however: when the network is heavily loaded so that NFS
starts to time out meaning that requests to create/write lockfiles stall and
when lock managers on machines remote from yours on the network begin to
hang (usually because of the NFS problems) meaning that acedb will be unable
to lock its lock files. In either case xace or tace will simply hang, i.e.
not respond to mouse clicks etc.. When this happens, all you can do is kill
the program using the Unix "kill" command, you will need to use "ps" to find
out the process id of the hung program and then type "kill -9 xxxxx" where
xxxxx is the process id. The acedb code cannot detect that this problem has
occurred because it cannot run while waiting to create/lock the lockfile.
Sadly there is nothing we can do to solve this problem.

gene finder bug
+++++++++++++++

There is a bug in the November code which may cause the "Gene finder" button
in fmap to crash the xace program. The problem happens when the definition
of the ?Method class in the models.wrm file in the users database does not
match the definition in the models.wrm file distributed with the code.
Previous versions of acedb did not detect this mismatch and continued to
run, probably with some errors in display of some fmap information.
Detection of this sort of parse error was tightened up recently to support
new function in AcePerl, hence the reason for the code failing now. Database
administrators should check that the definition of the ?Method class in
their models.wrm files matches that distributed with the code level they are
using.

We hope to make the code more tolerant of this problem in the future by
altering the code to check the model of the database against its method own
object and decide if it can continue if there is a difference. This will
probably not happen until the New Year.

Tab key:

There was a bug whereby if the user pressed the Tab key while inputting
text, the window would then no longer accept input. This has now been fixed
and tabs can be used as input to text boxes.

November monthly build now available.
*************************************

You can pick up the monthly builds from:

_Sanger users_
     ~acedb/RELEASE.DEVELOPMENT
_External users_
     ftp://ftp.sanger.ac.uk/pub/acedb/Sanger-release.2000_11_21.tar.gz

Next User Group Meeting - D213, 2.30pm, Thursday, 9th November
**************************************************************

----------------------------------------------------------------------------
----------------------------------------------------------------------------
Ed Griffiths <edgrif@sanger.ac.uk>
Last modified: Thu Nov 30 15:53:27 GMT 2000






From owner-acedb@hgmp.mrc.ac.uk  Fri Dec  1 13:20:48 2000
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From: Ed Griffiths <edgrif@sanger.ac.uk>
To: Acedb Newsletter <acedbg@sanger.ac.uk>,
	acedb newsgroup on bionet <bionet-software-acedb@net.bio.net>,
	aratnasiri@ucdavis.edu, boberkfe@watson.wustl.edu,
	Carson_Thoreen@brown.edu, dbaillie@gene.mbb.sfu.ca,
	dgrant@iastate.edu, G.REYES@CGIAR.ORG, janety@u.washington.edu,
	jayne.vallance@bbsrc.ac.uk, jmh.neefs@pandora.be,
	jspieth@watson.wustl.edu, jurg@sanger.ac.uk,
	keith@thale.life.nottingham.ac.uk, Lincoln Stein <lstein@cshl.org>,
	lnothake@watson.wustl.edu, lvandela@bccancer.bc.ca,
	matthews@greengenes.cit.cornell.edu, mieg@sanger.ac.uk,
	mummi@decode.is, mwilkinson@gene.pbi.nrc.ca, r.bruskiewich@CGIAR.ORG,
	sjones@bcgsc.bc.ca, sness@bcgsc.bc.ca, T.van_Wezel@lumc.nl
Subject: acedb newsletters....
Message-ID: <Pine.OSF.4.21.0012011024360.217-100000@griffin.sanger.ac.uk>
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Dear all,

There have been no newsletters for some time but now the silence is to be
broken. Sanger centre systems have been in some turmoil since the summer for a
number of reasons (flooding, new DEC clusters installed etc. etc.), this has
meant that we have not been able to reliably build/test acedb so I have been
reluctant to send out monthly releases. This in turn has meant that I have not
sent out the newsletters as there seemed little point in describing code changes
that were simply not available.

I have done three retrospective newsletters for Sept-Nov and these will be sent
out to you later today. As usual the newsletters can also be seen on the web
site (they will be there from tomorrow) at:

	http://www.acedb.org/winfo/Newsletters/

cheers Ed

 ------------------------------------------------------------------------
| Ed Griffiths, Acedb development, Informatics Group,                    |
|               The Sanger Centre, Wellcome Trust Genome Campus,         |
|               Hinxton, Cambridge CB10 1SA, UK                          |
|                                                                        |
| email: edgrif@sanger.ac.uk   URL: http://www.sanger.ac.uk/Users/edgrif |
|   Tel: +44-1223-494780       Fax: +44 1223 494919                      |
 ------------------------------------------------------------------------






From owner-acedb@hgmp.mrc.ac.uk  Fri Dec  1 13:21:01 2000
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From: Ed Griffiths <edgrif@sanger.ac.uk>
To: Acedb Newsletter <acedbg@sanger.ac.uk>,
	acedb newsgroup on bionet <bionet-software-acedb@net.bio.net>,
	aratnasiri@ucdavis.edu, boberkfe@watson.wustl.edu,
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	r.bruskiewich@CGIAR.ORG, sjones@bcgsc.bc.ca, sness@bcgsc.bc.ca,
	T.van_Wezel@lumc.nl
Subject: October newsletter
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and the October newsletter....

 ------------------------------------------------------------------------
| Ed Griffiths, Acedb development, Informatics Group,                    |
|               The Sanger Centre, Wellcome Trust Genome Campus,         |
|               Hinxton, Cambridge CB10 1SA, UK                          |
|                                                                        |
| email: edgrif@sanger.ac.uk   URL: http://www.sanger.ac.uk/Users/edgrif |
|   Tel: +44-1223-494780       Fax: +44 1223 494919                      |
 ------------------------------------------------------------------------


----------------------------------------------------------------------------
----------------------------------------------------------------------------

ACEDB User Group Newsletter - October 2000
##########################################

If you want to have this newsletter mailed to you _or_ you want to make
comments/suggestions about the format/content then send an email to
acedb@sanger.ac.uk.

Like last month, this month has been a month of bug fixes in preparation for
the release of Acedb 4_9.

New Features
************

Acedb MS-Windows CD packaging improvements
++++++++++++++++++++++++++++++++++++++++++

The following improvements have been made to the Install process:

   * - there's a new database wizard in the for the installation, it will
     install the acedb binaries and set up a new/empty default database.
   * - the file extension ".wrm" is automatically registered with MS-Windows
     to be opened with MS-Windows Notepad as this is a suitable editor for
     altering them.
   * - The latest Worm WS releases are now regularly packaged with the Acedb
     MS-Windows CD.

_Coming soon -_ at the Acedb web site, a document on how to make windows
packages, databases, cds, for acedb.

URLs on Acedb MS-Windows
++++++++++++++++++++++++

URLs in objects now work correctly in the MS-Windows version of acedb, the
URL will be displayed by whichever program is registered with MS-Windows to
deal with URLs (this might be Internet Explorer or maybe Netscape).

".wrm" files on Acedb MS-Windows
++++++++++++++++++++++++++++++++

A problem with Unix vs. MS-Windows is that they signal the end of a line of
text in a different way. This matters if you edit one of acedbs text files
(e.g. models.wrm) on Windows and then try to edit again on a Unix machine.
Acedb expects ".wrm" text files to use the Unix way of representing an
end-of-line, but the MS-Windows version will also cope with the MS-Windows
way of doing this. This means that Acedb will continue to work if you have
editted your models.wrm file on an MS-Windows machine.

Bugs Fixed
**********

"%" Dumping bug
+++++++++++++++

acedb 4_8 had a bug whereby "%" signs within data in the database e.g. "GC
content 48%" would not be dumped correctly because the "%" was read as a
formatting instruction. This is now fixed.

Incorrect ordering of output to screen under tace
+++++++++++++++++++++++++++++++++++++++++++++++++

Error messages in tace could get tangled with the "acedb>" prompt which was
confusing. This has now been fixed.

Loss of window focus
++++++++++++++++++++

Sometimes when acedb popped up messages (warnings, information etc.), this
could lead to the acedb losing the keyboard focus, i.e. any typing by the
user would be lost. This is now fixed.

No monthly build available.
***************************

Unfortunately, due to system problems at the Sanger Centre a monthly build
has not been possible for September.

Next User Group Meeting - D213, 2.30pm, Thursday, 12th October
**************************************************************

----------------------------------------------------------------------------
----------------------------------------------------------------------------
Ed Griffiths <edgrif@sanger.ac.uk>
Last modified: Thu Nov 30 15:41:36 GMT 2000






From owner-acedb@hgmp.mrc.ac.uk  Mon Dec  4 07:57:53 2000
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To: T.van_Wezel@lumc.nl, acedbg@sanger.ac.uk,
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Subject: Re:  November newsletter
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Thanks Ed for all your newsletters and updates.

I have one comment about the outstanding issue on 

xace/tace hang when trying to get write access
++++++++++++++++++++++++++++++++++++++++++++++

which I think is primarily a Sanger Centre problem.  As Ed says, this
is due to NFS problems.  The correct way to avoid this problem is to
ALWAYS run acedb from the machine that directly mounts the disk with
the database on.  This not only deals with NFS problems, but will also
improve performance substantially.  I'm going to copy copy Kate Rice
here who oversees all the humgen informatics operations, because this
may require changing some LSF behaviour for the humgen cluster.  We
have talked about this before....

Richard





From owner-acedb@hgmp.mrc.ac.uk  Mon Dec  4 15:56:30 2000
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From: "F. Coulier" <coulier@marseille.inserm.fr>
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Hello to all,

I have been trying for quite some time to move Acedb (plus other
databases that run with acedb) from one computer (linux mandrake 6.1) to
another (linux mandrake 7.1), but I have lots of difficulties setting up
web access.

I can run acedb on the new machine OK, as well as 2 others small
databases we are (trying to) develop in the lab.

On the "new" computer, I can manually start gifaceserver to run any of
these 3 databases (i.e. fc]$ gifaceserver /database/acedb 20114
1200:1200:100)
and then run aceclient (i.e. fc]$ aceclient xxx.xxx.xxx.xxx -port 20114)
from the PC where the gifaceserver is running, or from another one.

Now, where I failed, is when I try to start the gifaceserver on the new
computer by launching the aceclient (this works Ok on the "old" PC).

I suspect a file permission problem, but cannot find where this problem
could be.
gifaceserver is executable by user acedb and group U119
fc (me, the username underwhich I tried to run aceclient) is a member of
group U119.

In inetd.conf I have:
acedb/1 stream  rpc/tcp wait    acedb.U119
/usr/local/bin/rpc.gifaceserver rpc.gifaceserver /database/acedb 20114
1200:1200:0

rpc.gifaceserver is a symbolic link to gifaceserver

/database/acedb and /database/acedb/database are rwx for acedb.U119

Any help or clues would be greatly appreciated!

François

-- 
François Coulier,        mél: coulier@marseille.inserm.fr
INSERM Unité 119, 27 bd Leï Roure, 13009 Marseille, France 
Tel: 33 (0) 4 91 26 04 26 (temporaire) Fax: 33 (0) 4 91 26 03 64
Site ouaibe: http://olan.marseille.inserm.fr/





From owner-acedb@hgmp.mrc.ac.uk  Mon Dec  4 16:26:53 2000
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To: bionet-software-acedb@net.bio.net
From: Ed Griffiths <edgrif@sanger.ac.uk>
Newsgroups: bionet.software.acedb
Subject: Re: Still have prblems settings up things right
Organization: Sanger Centre
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Francois,

First a couple of questions:

1) What level of acedb are you using ?  If you are using 4_8, you should try
the    socket server rather than the RPC server. The programs are called
saceserver     and saceclient and details for set up are in wdoc.

2) What are your entries in  /etc/services  /etc/rpc  /etc/inetd  ??


Some suggestions

Have you looked in the server log or acedb log in the database to see if the
server program even starts up ?  If not, then its likely to be a problem either
with the /etc files or with permissions.

Are you trying to get write access or have you altered the server.wrm file to
allow universal reading (this is a good idea just to get things started).

Don't know if any of this helps....

cheers Ed

 ------------------------------------------------------------------------
| Ed Griffiths, Acedb development, Informatics Group,                    |
|               The Sanger Centre, Wellcome Trust Genome Campus,         |
|               Hinxton, Cambridge CB10 1SA, UK                          |
|                                                                        |
| email: edgrif@sanger.ac.uk   URL: http://www.sanger.ac.uk/Users/edgrif |
|   Tel: +44-1223-494780       Fax: +44 1223 494919                      |
 ------------------------------------------------------------------------





From owner-acedb@hgmp.mrc.ac.uk  Mon Dec  4 19:03:34 2000
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From: Vitali Proutski <V.Proutski@har.mrc.ac.uk>
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Hey there,
I'm having a problem with displaying graphics in AceBrowser.  The
asGig() method from Ace::Object.pm seems to quit earlier than it is
supposed to.  If  I woiuld love to hear from anybody how had similar
type of problem and solved it.

Vitali.






From owner-acedb@hgmp.mrc.ac.uk  Tue Dec  5 16:06:19 2000
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Ed Griffiths a écrit :

> Francois,
>
> First a couple of questions:
>
> 1) What level of acedb are you using ?  If you are using 4_8, you should try
> the    socket server rather than the RPC server. The programs are called
> saceserver     and saceclient and details for set up are in wdoc.
>

I use 4_7. Do you think I should  update to 4_8, or wait for 4_9 ?


>
> 2) What are your entries in  /etc/services  /etc/rpc  /etc/inetd  ??
>

/etc/services:
acedb           20114/tcp
nbdb            20117/tcp
madb            20118/tcp

/etc/rpc:
acedb           20114 rpc.gifaceserver
nbdb            20117 rpc.gifaceserver
madb            20118 rpc.gifaceserver

/etc/inetd.conf:
acedb/1 stream  rpc/tcp wait    acedb.U119      /usr/local/bin/rpc.gifaceserver
rpc.gifaceserver /database/acedb 20114 1200:1200:0
nbdb/1  stream  rpc/tcp wait    acedb.U119      /usr/local/bin/rpc.gifaceserver
rpc.gifaceserver /database/Nbdb 20117 1200:1200:0
madb/1  stream  rpc/tcp wait    acedb.U119        /usr/local/bin/rpc.gifaceserver
rpc.gifaceserver /database/madb 20118 1200:1200:0



>
> Some suggestions
>
> Have you looked in the server log or acedb log in the database to see if the
> server program even starts up ?  If not, then its likely to be a problem either
> with the /etc files or with permissions.
>

There is no traces in server.log. My guess is that the server does not start at
all.

I actually get the following message when trying to launch :aceclient
[fc@grun fc]$ aceclient xxx.xxx.xxx.xxx -port 20114
//! cannot establish connection
//! usage: aceclient host [-port number] [-time_out secs] [-access_info]
[-ace_out] [-ace_in] [-f reportfile parameters]


>
> Are you trying to get write access or have you altered the server.wrm file to
> allow universal reading (this is a good idea just to get things started).

I have WRITE_ACCESS_DIRECTORY /database/Write_access
With the proper permission set up for this directory (tried rwx for all)

I have also READ_ACCESS_DIRECTORY PUBLIC

Thanks for your help!

François





From owner-acedb@hgmp.mrc.ac.uk  Wed Dec 13 14:32:12 2000
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From: Ed Griffiths <edgrif@sanger.ac.uk>
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Calling all acedb installers...

I am making some changes to the acedb INSTALL script in preparation for the next
release of acedb.

The old script used to:

install source in ./w*, binaries in ./bin, docs in ./w*, install a version of
the worm database and mess about with various files in the worm database. The
script also created a couple of small scripts that would invoke xace/tace on the
worm database.

Its no longer practical to distribute the worm database with acedb source code,
its just too big. If you want the worm database, please can you now go to one of
the below for this:

http://www.sanger.ac.uk/Projects/C_elegans/WORMBASE/
ftp://ftp.sanger.ac.uk/pub/wormbase

I propose that the INSTALL script should now be a more simple affair that
unpacks source, binary and/or docs archives into the current directory and makes
any links required for building the code and makes sure that files have the
correct permissions.

Is this acceptable ? Are there any other functions that the script should
perform that are vital to users ?

cheers Ed

 ------------------------------------------------------------------------
| Ed Griffiths, Acedb development, Informatics Group,                    |
|               The Sanger Centre, Wellcome Trust Genome Campus,         |
|               Hinxton, Cambridge CB10 1SA, UK                          |
|                                                                        |
| email: edgrif@sanger.ac.uk   URL: http://www.sanger.ac.uk/Users/edgrif |
|   Tel: +44-1223-494780       Fax: +44 1223 494919                      |
 ------------------------------------------------------------------------





From owner-acedb@hgmp.mrc.ac.uk  Mon Dec 18 10:48:45 2000
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To: bionet-software-acedb@net.bio.net
From: John Sturdy <jcgs@sanger.ac.uk>
Newsgroups: bionet.software.acedb
Subject: AceDB Showcase
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I'm preparing the new version of www.acedb.org, and we'd like to
include an examples / showcase section, linking to various projects
that use AceDb. If any of you are prepared to have your project
included in this, please mail me the URL of a page describing it, and
I'll include it.

__John

-- 
Dr John Sturdy                                            jcgs@sanger.ac.uk
Informatics Division
Sanger Centre





From owner-acedb@hgmp.mrc.ac.uk  Mon Dec 18 12:21:18 2000
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Although I mentioned John in the November newsletter, for those of you that
missed that item: John is a new acedb developer and he's starting off by sorting
out our website and downloads sections.

I noted a number of suggestions from the Acedb Vancouver meeting earlier this
year (which John is implementing), if you have any comments about the acedb web
or download sites then do please either post them here or send them to John
direct.

cheers Ed

 ------------------------------------------------------------------------
| Ed Griffiths, Acedb development, Informatics Group,                    |
|               The Sanger Centre, Wellcome Trust Genome Campus,         |
|               Hinxton, Cambridge CB10 1SA, UK                          |
|                                                                        |
| email: edgrif@sanger.ac.uk   URL: http://www.sanger.ac.uk/Users/edgrif |
|   Tel: +44-1223-494780       Fax: +44 1223 494919                      |
 ------------------------------------------------------------------------





From owner-acedb@hgmp.mrc.ac.uk  Fri Dec 22 13:22:35 2000
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From: John Sturdy <jcgs@sanger.ac.uk>
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Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
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As I'm nearing the end (I think) of the first batch of my work on the
website, I'm beginning to think ahead to making use of emacs to help
with working with AceDB -- I do nearly all my work through emacs, and
often write bits of elisp to help out.

If any of you have any requests for what you'd like included in an
emacs package for working with AceDB, or have any pieces of existing
code you'd like to contribute, let me know in the next few weeks and
I'll try to include them.

What I have in mind includes:
  editing modes for model files and ace files
  specialized comint (shell interaction) for driving tace, with emacs
    menu entries, and possibly with syntax colouring of results

The editing mode for model files will parse the model file to know
what tags are defined, etc, and the mode for ace files will be able to
make use of this data (if told which model to use), providing
completion on tag names, checks for valid tags on saving the file, etc.

Comments? Discussion?

I'll put the resulting code on the acedb.org website.

(If anyone's interested, I can also put the elisp I've used to re-work
the website on-line, probably in my webspace on sanger.ac.uk)

-- 
Dr John Sturdy                                            jcgs@sanger.ac.uk
Informatics Division
Sanger Centre





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From: Keith Bradnam <keith@thale.life.nottingham.ac.uk>
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A while ago I asked if anyone knew if the AceBrowser scripts used on the
wormbase site were publically available.

I spoke to Lincoln Stein a couple of weeks ago and he informed me that the
scripts are available from the wormbase ftp site at:

ftp.wormbase.org

need to look in the /pub/wormbase directory and grab the
wormbase-site.tar.gz file which contains everything.


Keith

~  Keith Bradnam - UK CropNet (http://ukcrop.net) webmaster and
~  Arabidopsis Genome Resource  (http://ukcrop.net/agr) developer.
~
~  Nottingham Arabidopsis Stock Centre, University Park,
~  University of Nottingham, NG7 2RD, UK.  Tel: (0115) 951 3091 






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From: Ed Griffiths <edgrif@sanger.ac.uk>
To: Acedb Newsletter <acedbg@sanger.ac.uk>,
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	aratnasiri@ucdavis.edu, boberkfe@watson.wustl.edu,
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	sjones@bcgsc.bc.ca, sness@bcgsc.bc.ca, T.van_Wezel@lumc.nl
Subject: December newsletter
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As promised, the December newsletter.

Please note that the December monthly build will not be available until the
first week in January, sorry, there just isn't time to test it before Christmas.

cheers Ed

 ------------------------------------------------------------------------
| Ed Griffiths, Acedb development, Informatics Group,                    |
|               The Sanger Centre, Wellcome Trust Genome Campus,         |
|               Hinxton, Cambridge CB10 1SA, UK                          |
|                                                                        |
| email: edgrif@sanger.ac.uk  Tel: +44-1223-494780  Fax: +44-1223-494919 |
 ------------------------------------------------------------------------


----------------------------------------------------------------------------
----------------------------------------------------------------------------

ACEDB User Group Newsletter - December 2000
###########################################

If you want to have this newsletter mailed to you _or_ you want to make
comments/suggestions about the format/content then send an email to
acedb@sanger.ac.uk.

This month sees: the announcement of ChickAce, a reminder for Unix users
about environment limits and "out of memory" errors from Acedb, major
reworking of the Acedb website, quite a number of bug fixes, and a proposal
for some new variations of the Rename function.

General News
************

ChickAce
++++++++

Martien Groenen emailed to say that he has been setting up an acedb database
for the chicken:

From: Martien.Groenen@Alg.VF.WAU.NL

During the last 6 months I have set up ChickAce which now contains almost all
the mapping information that is available for chicken including the linked
data such as references (abstracts), sequences, Comparative mapping
information (human and mouse), primers, SNP's etc.
Currently the database is running satisfactory under Windows in our lab, and
someone within my group is using the available CGI script to make the database
available on our website (hopefully running in the beginning of next year).

In one of the latest newsletters I also saw that URL's are now recognized
correctly from within AceDb. Can I use that to link data directly to other
databases that are available through the WWW (e.g. for all genes in ChickAce I
have also added an extra entry for the Locuslink ID for human and mouse. Can I
make a direct link with Locuslink?).

Could you add me to the mailing list for the AceDb newsletter (you could also
mention that an AceDb database for chicken has been set up in wageningen).

Best regards,
Martien

Articles
********

User Environment Limits and Acedb on Unix
+++++++++++++++++++++++++++++++++++++++++

This is a brief reminder about user limits which can cause acedb to crash if
they are set too low ( applies to unix not MS-Windows users).

All users who log on to a unix box (whether directly or via a network
terminal) have a set of user limits which control the amount of memory/files
etc. that they are allowed to use. You can look at these limits using the C
shell command "limit":

> limit
cputime         unlimited
filesize        unlimited
datasize        262144 kbytes
stacksize       2048 kbytes
coredumpsize    0 kbytes
memoryuse       505664 kbytes
descriptors     4096

"datasize" and "stacksize" particularly affect Acedb and can cause it to
crash by running out of memory. In the above example "stacksize" is about
2MB which is too low, whereas "datasize" is fine for most uses at about
260MB.

If Acedb crashes unexpectedly with either of these warnings:

"Memory allocation failure when acedb requested NNNNNN bytes,
 acedb has already allocated NNNNNN bytes...."

or

"ABORT : Fatal program fault, signal NN...."

then you should try increasing your limits using the "unlimit" C shell
command:

> unlimit stacksize
> unlimit datasize

If this does not fix the problem then report it either using the ddts bug
reporting system:

   * http://www.sanger.ac.uk/cgi-bin/webddts/WebDDTS.pl?Project=Acedb

or by sending a description of the problem to the acedb developers at:

   * acedb@sanger.ac.uk

Acedb Web Pages re-organisation
+++++++++++++++++++++++++++++++

John Sturdy has been undertaking a major reorganisation of the acedb web
pages and download site. If you visit the Acedb website you will see that
the front page has changed and will continue to change quite radically until
about mid-January.

Of special interest are:

   * Separate "User", "Administrator/Curator" and "Developer" sections.
   * A Search box which allows you to make keyword searches of acedb
     documents both on the Acedb website and the Acedb document library in
     the States. Please do try this and if you find notable omissions then
     either send mail to the Acedb newsgroup or to acedb@sanger.ac.uk.
   * There is a new "downloads" set of pages under construction to give
     better access to the ftp site (available in January).
   * There is a new "Site map" link which gives a quick overview of the
     document structure of the Acedb site as a whole.

John will be adding a lot of new material to the website over the next
couple of weeks and our intention is to make it a lot more usable. Comments
or suggestions are welcome, please send them to the Acedb newsgroup or
acedb@sanger.ac.uk.

New Features
************

New position readout in fmap
++++++++++++++++++++++++++++

A small change courtesy of Jean Thierry-Mieg, fmap has a friendlier readout
for the position on the sequence which will now be in kilo (kb) and Mega
(Mb) bases, making it a little easier to read than 1238749217498123794 as
used to be displayed for long sequences.

Better diagnotics from Rename/Alias failures
++++++++++++++++++++++++++++++++++++++++++++

Previously Acedb has not reported the reason for these failures, only that
they happened, now parsing will report in detail the reason for any Rename
or Alias failures so that fixing them should be easier.

Bugs Fixed
**********

Colours bug with gifs
+++++++++++++++++++++

GIF images produced by Acedb 4_8c were sometimes in garish/hard to read
colours, this is now fixed in the December monthly build.

Dumping objects with very large tag values
++++++++++++++++++++++++++++++++++++++++++

Some tag values can be very large (e.g. DNA) and this has caused acedb to
overrun one of its internal buffers, this is now fixed with dynamically
growing buffers.

File Chooser irritations
++++++++++++++++++++++++

Several irritating features of the File Chooser window have been fixed:

   * If you want to go directly to a directory, you can do this by typing
     the directory name in the bottom "selection" window and pressing the
     <tab> key. This will point the file chooser to this directory so you
     can then choose files from there. The <tab> key also does filename
     completion like the C shell.
   * The File Chooser window now shows a "busy" cursor when it cannot
     respond to user input e.g. because its waiting to list an NFS mounted
     directory.
   * The default size of the window has been increased to make it easier to
     see long filenames.

Cut/Paste bug fixed
+++++++++++++++++++

Cut and Paste of larger texts (e.g. DNA) was broken, this was a bug in the
underlying Window System software (GTK) and is being fixed by installing
updated versions of the software which include the bug fix.

Main Window reset after "Clean Up"
++++++++++++++++++++++++++++++++++

"Clean up" behaviour has been returned to how it was in 4_7, the
local/global search is left as it was before the "Clean up".

Socket Server Admin users permissions bug
+++++++++++++++++++++++++++++++++++++++++

If you noticed problems with Admin users not having access to some commands
for the socket server, this is now fixed. Admin users should have access to
all possible commands as they have the highest permission levels.

Education
*********

HGMP are hosting an Acedb course in the week of 22nd-26th January 2001.

The next Sanger Centre course is likely to be in June 2001.

If you are interested in either please mail Sylvia Martinelli
(sylvia@sanger.ac.uk)

Future Plans
************

If you wish to make suggestions about any of the below, please mail them to
acedb@sanger.ac.uk

Revised INSTALL script for Acedb
++++++++++++++++++++++++++++++++

The current INSTALL script for acedb is being revised, the section for
installing the worm database will be moved into a separate script (available
from the worm web site in the January). The download site will include for
each acedb release the following archives: compilable source code, binaries
for selected platforms, help pages, docs and a test database.

Enhancements to "Rename"
++++++++++++++++++++++++

Discussions with a number of users have suggested that the "Rename"
operation doesn't always so what they would like or expect. Currently it
works like this:

Let's say we wish to rename Sequence object "A" to "B":

If the database does _not_ contain a Sequence object with the name "B", the
operation is straight forward: object "A" is simply given the new name of
"B" and the name "A" ceases to exist.

If the database _does_ contains a Sequence object with the name "B", things
are more complicated, the two objects are combined to form a new "B" object
following these rules:

                                                    value used for
tag type   tag in "A" ?    tag in "B" ?             tag in new "B"
--------   ------------    ------------             --------------
UNIQ           Y               N                      "A"s value
               N               Y                      "B"s value
               Y               Y                      "A"s value

non-UNIQ       Y               N                      "A"s values
               N               Y                      "B"s values
               Y               Y                   (all of "A"s values
                                                   + all of "B"s values)

In this case the original "A" and "B" objects will be replaced with the
single new "B" object.

The new proposal is:

Given that we want to rename object "A" to "B" and that "B" already points
to an object:

  1. The existing rename operation will combine "A" and "B" as before.
  2. New variants of rename will be provided:
        o safe-rename: will fail because "B" points to an existing object
        o replace-rename: will delete "B" and then rename "A" to "B"
        o overwrite-rename: will combine "A" and "B", where tags exist in
          both "A" will take preference.
        o augment-rename: as for overwrite-rename, but where tags exist in
          both "B" will take preference.
     When implemented, the third and fourth operations will probably be
     called "Merge-nnn".

This would allow users to do renames and choose how this should work. They
could for instance do a "safe-rename" which would avoid any loss of data, or
conversely do a "replace-rename" which would avoid any combining of objects.

A question remains about whether these operations should be inclusive or
exclusive for non-UNIQ tags, i.e. should they be combined as before, or
should one set of tags overwrite another. If you are regularly parsing in
data to an Acedb database and have thoughts about how you would like Rename
to work, then please mail acedb@sanger.ac.uk.

December monthly build available in first week of January 2001
**************************************************************

Sorry, not time to do this before Christmas, build has been done and will be
distributed first week in January.

You can pick up the monthly builds from:

_Sanger users_
     ~acedb/RELEASE.DEVELOPMENT
_External users_
     ftp://ftp.sanger.ac.uk/pub/acedb/Sanger-release.2000_12_22.tar.gz

Next User Group Meeting - D319, 2.30pm, Thursday 18th January 2001
******************************************************************

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Ed Griffiths <edgrif@sanger.ac.uk>
Last modified: Fri Dec 22 17:38:09 GMT 2000






