From owner-ageing@net.bio.net Tue Feb 01 22:00:00 1994 Path: biosci!daresbury!not-for-mail From: olxpa01@mailserv.zdv.uni-tuebingen.de (Graham Pawelec) Newsgroups: bionet.molbio.ageing Subject: Debate_on_telomerase Date: 2 Feb 1994 20:51:12 -0000 Lines: 82 Sender: daemon@mserv1.dl.ac.uk Distribution: bionet Message-ID: <2ip3o0$gbc@mserv1.dl.ac.uk> Original-To: AGEING@dl.ac.uk Concerning the recent debate on telomerase, may I summarize (so that experts can correct me!) and ask a couple of questions? The TELOMERE HYPOTHESIS of cellular ageing proposes that loss of telomeric DNA, and gradual shortening of telomeres, results, after a certain number of cell divisions, in the inability of cells to divide again(1). Loss of telo- meric DNA has been demonstrated in blood cells in vivo, and has been shown to occur more rapidly in premature ageing syndromes, eg. Hutchinson-Gilford progeria(2) or trisomy-21(3). These investigators also examined lymphocytes from normal donors cultured for 10 - 30 PD, and estimated a telomere DNA loss rate of 120 bp/cell doubling, comparable to that seen in other somatic cells. Moreover, telomere lengths from lymphocytes of centenarians were si- milar to those of senescent cells in culture, which suggests that telomere shortening may indeed by of relevance to normal immunosenescence(3). More- over, telomere length in sperm DNA did NOT decrease with increasing age of the donor, suggesting that a mechanism for maintaining telomere length may be active in germ cells but not somatic cells(2,4). Such a factor, telome- rase, an enzyme responsible for maintaining telomere length in unicellular eukaryotes, has been found in immortalized human cell lines and tumour cells, but not in normal somatic cells. Telomerase may be an obligate but late event in transformation, because transformed cells and tumour tissues generally have critically short telomeres(5). However, it seems to me that the telomerase hypothesis is difficult to re- concile with the dominance of the senescent state over the proliferative state in heterokaryons. A possible way out of this dilemma was suggested by Shay et al(6) who pointed out that this could be explained by postulating dominant telomerase repressors in normal cells, which are lost in immortal cells. This hypothesis has not yet been experimentally tested - or could somebody tell me whether it has been in the meantime?. I also think that arguing against telomere shortening as a general mechanism of ageing is the finding that, whereas senescence and immunosenescence are well-recognized occurrences even in short-lived species such as mouse, telomere lengths are NOT decreased during murine ageing, and neither are sperm telomeres longer than somatic tissue telomeres7. Isn't it unlikely that phenotypically simi- lar ageing processes in human and mouse would involve telomere shortening in the former but not the latter? References 1. Harley CB, Futcher AB, Greider CW. Telomeres shorten during ageing of human fibroblasts. Nature 1990;345:458-60. 2. Allsopp RC, Vaziri H, Patterson C, et al. Telomere Length Predicts Replicative Capacity of Human Fibroblasts. Proc Natl Acad Sci USA 1992;89:10114-8. 3. Vaziri H, Schachter F, Uchida I, et al. Loss of Telomeric DNA During Aging of Normal and Trisomy-21 Human Lymphocytes. Am J Hum Genet 1993;52(4):661-7. 4. Hastie ND, Dempster M, Dunlop MG, Thompson AM, Green DK, Allshire RC. Telomere reduction in human colorectal carcinoma and with ageing. Nature 1990;346:866-8. 5. Harley CB. Telomere Loss - Mitotic Clock or Genetic Time Bomb. Mutat Res 1991;256:271-82. 6. Shay JW, Wright WE, Werbin H. Loss of Telomeric DNA During Aging May Predispose Cells to Cancer (Review). Int J Oncol 1993;3(4):559-63. 7. Kipling D, Cooke HJ. Hypervariable ultra-long telomeres in mice. Nature 1990;347:400-2. ############################################################ # # # Graham Pawelec, # # Second Department of Internal Medicine, # # University of Tuebingen Medical School, # # D-72076 Tuebingen, Germany; # # # # Coordinator, European Union Concerted # # Action on the Molecular Biology of # # Immunosenescence, EUCAMBIS. # # # # e-mail INTERNET: pawelec@mailserv.zdv.uni-tuebingen.de # # # # Phone: +49-7071-29-2805 # # # # FAX: +49-7071-29-4464 # # # ############################################################ From owner-ageing@net.bio.net Fri Feb 04 22:00:00 1994 Newsgroups: bionet.molbio.ageing Path: biosci!daresbury!doc.ic.ac.uk!uknet!pipex!howland.reston.ans.net!vixen.cso.uiuc.edu!uchinews!news From: dscohen@midway.uchicago.edu (David Spencer Cohen) Subject: Re: Debate_on_telomerase Message-ID: <1994Feb4.231937.18126@midway.uchicago.edu> X-Posted-From: InterNews 1.0.1b7@bert1.rh.uchicago.edu Lines: 8 Sender: news@uchinews.uchicago.edu (News System) Organization: University of Chicago References: <2ip3o0$gbc@mserv1.dl.ac.uk> Date: Fri, 4 Feb 1994 23:19:37 GMT Xdisclaimer: No attempt was made to authenticate the sender's name. That seemed very thorough and certainly interesting. I'm not sure I'd be quite so dismissive, though. Considering ageing as a multitude of entropic mechanisms of different relative importances acting upon the organismal genome, it is not necessary that the subtle phenotypic differences in ageing be correlated to differences (or similarities) in molecular processes. It is possible that longer-lived mammals make use of telomerase repressor lifespan regulation whereas some other primary mechanism is more evolutionarily appropriate for, say, murine species. From owner-ageing@net.bio.net Fri Feb 04 22:00:00 1994 Path: biosci!bcm!cs.utexas.edu!uunet!news.sprintlink.net!news.clark.net!gog From: gog@clark.net (Charles Lancelotta) Newsgroups: bionet.molbio.ageing Subject: what about mort 1,2; antoxidants? Date: 5 Feb 1994 05:59:25 GMT Organization: Clark Internet Services, Inc., Ellicott City, MD USA Lines: 1 Message-ID: <2ivcjt$79r@clarknet.clark.net> NNTP-Posting-Host: explorer.clark.net Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit X-Newsreader: TIN [version 1.2 PL2] From owner-ageing@net.bio.net Sun Feb 06 22:00:00 1994 Path: biosci!daresbury!doc.ic.ac.uk!agate!howland.reston.ans.net!cs.utexas.edu!uunet!athos.cc.bellcore.com!fubar!andrew From: andrew@fubar.NoSubdomain.NoDomain (Andrew Verba) Newsgroups: bionet.molbio.ageing Subject: you are a retard Date: 6 Feb 1994 18:58:05 GMT Organization: Bell Communications Research Lines: 2 Sender: andrew@fubar (Andrew Verba) Distribution: world Message-ID: <2j3ejt$s7l@athos.cc.bellcore.com> NNTP-Posting-Host: fubar.ccs.bellcore.com what can i say, Marko Kapalla is a retard? From owner-ageing@net.bio.net Fri Feb 11 22:00:00 1994 Path: biosci!NET.BIO.NET!biosci-help From: biosci-help@NET.BIO.NET (BIOSCI Administrator) Newsgroups: bionet.molbio.ageing Subject: test of ageing@net.bio.net Date: 12 Feb 1994 02:19:13 -0000 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: bionet Message-ID: Reply-To: biosci-help@net.bio.net NNTP-Posting-Host: net.bio.net test of ageing@net.bio.net From owner-ageing@net.bio.net Mon Feb 14 22:00:00 1994 Path: biosci!parc!decwrl!ames!agate!howland.reston.ans.net!newsserver.jvnc.net!raffles.technet.sg!nuscc.nus.sg!eng10345 From: eng10345@leonis.nus.sg (Pan Keung) Newsgroups: bionet.molbio.ageing Subject: Re: test of ageing@net.bio.net Date: 15 Feb 1994 03:19:55 GMT Organization: National University of Singapore Lines: 6 Distribution: bionet Message-ID: <2jpf0r$ago@nuscc.nus.sg> References: NNTP-Posting-Host: leonis.nus.sg X-Newsreader: TIN [version 1.2 PL0] BIOSCI Administrator (biosci-help@NET.BIO.NET) wrote: : test of ageing@net.bio.net : just testing From owner-ageing@net.bio.net Tue Feb 15 22:00:00 1994 Newsgroups: bionet.molbio.ageing Path: biosci!parc!decwrl!ames!elroy.jpl.nasa.gov!sdd.hp.com!saimiri.primate.wisc.edu!xanth.cs.odu.edu!concert!news-feed-1.peachnet.edu!umn.edu!maroon.tc.umn.edu!siems001 From: siems001@maroon.tc.umn.edu (Dann Paul Siems) Subject: Is this newsgroup active? Message-ID: Summary: I am wondering if this group is worth adding to my .newsrc file Sender: news@news.cis.umn.edu (Usenet News Administration) Nntp-Posting-Host: maroon.tc.umn.edu Organization: University of Minnesota, Twin Cities Date: Wed, 16 Feb 1994 01:33:49 GMT Lines: 10 Is anybody out there reading this newsgroup? I'd be interested in participating in any dialogues that might develop especially regarding the current thinking regarding antagonistic pleitropy. Thanks. -- =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Dann Siems | Ecology/Evolution/Behavior | Without speculation there can be no good University of Minnesota | and original observation. Saint Paul MN 55108 | =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= From owner-ageing@net.bio.net Fri Feb 18 22:00:00 1994 Newsgroups: bionet.molbio.ageing Path: biosci!daresbury!doc.ic.ac.uk!uknet!pipex!uunet!newsgate.watson.ibm.com!hawnews.watson.ibm.com!earth!soreff From: soreff@watson.ibm.com (Jeffrey Soreff) Subject: Missing subject header Sender: news@watson.ibm.com (News Poster) Message-ID: <1994Feb19.021143.17930@watson.ibm.com> Date: Sat, 19 Feb 1994 02:11:43 GMT Reply-To: soreff@fshvmfk1.vnet.ibm.com Disclaimer: This posting represents the poster's views, not necessarily those of IBM Nntp-Posting-Host: arizona.fishkill.ibm.com Organization: IBM East Fishkill Lines: 8 Just out of curiosity: What is the approximate total funding of fundamental studies on the mechanism(s) of ageing? I have no feeling for how active this field is. Thanks in advance for any information. -Jeffrey Soreff standard disclaimer: I do not speak for my employer. From owner-ageing@net.bio.net Fri Feb 18 22:00:00 1994 Newsgroups: bionet.molbio.ageing From: jm@ambident.demon.co.uk (Jonathan Montagu) Path: biosci!daresbury!doc.ic.ac.uk!uknet!pipex!demon!ambident.demon.co.uk!jm Subject: Info required Lines: 23 X-Newsreader: Archimedes ReadNews Date: Sat, 19 Feb 1994 14:33:56 +0000 Message-ID: <6PjtIEj024n@ambident.demon.co.uk> Sender: usenet@demon.co.uk I'm writing an essay on the applications of genetic manipulation in humans for general discussion. It's an interesting idea that it might be possible remove or attenuate the genes responsible for ageing and hence prolong lives. I was wondering whether anybody can offer any suggestions. Particularly: 1. What do the 'ageing' genes code for in a multicellular organism such as a human? 2. At a cellular level I understand that if cells do not receive certain social signals death occurs. What about on a multicellular level - why do organisms deteriorate over time? 3. What might be the social consequences of prolonged lifetimes? Thanks in advance ------ | | |onathan Montagu __/ ----------------------------------------------------------------------------- Internet: jm@ambident.demon.co.uk | 'Murphy's Law of Thermodynamics: Arcade: User #677, J.MONTAGU | Things get worse under pressure' Digital Databank: User #330, J.MONTAGU | ----------------------------------------------------------------------------- From owner-ageing@net.bio.net Sat Feb 19 22:00:00 1994 Path: biosci!NETCOM.COM!wick From: wick@NETCOM.COM (Potter Wickware) Newsgroups: bionet.molbio.ageing Subject: Re: Missing subject header Date: 20 Feb 1994 00:05:26 -0000 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: bionet Message-ID: References: <1994Feb19.021143.17930@watson.ibm.com> NNTP-Posting-Host: net.bio.net Kinda hard to gauge activity of the field by funding, I would think. Why don't you search Medline for journal articles, by keyword and year? PW. On Sat, 19 Feb 1994, Jeffrey Soreff wrote: > Just out of curiosity: > What is the approximate total funding of fundamental studies > on the mechanism(s) of ageing? I have no feeling for how > active this field is. Thanks in advance for any information. > > -Jeffrey Soreff > > standard disclaimer: I do not speak for my employer. > > From owner-ageing@net.bio.net Sat Feb 19 22:00:00 1994 Newsgroups: bionet.molbio.ageing Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!usenet.ins.cwru.edu!magnus.acs.ohio-state.edu!csn!boulder!cnsnews!ucsu.Colorado.EDU!fugate From: fugate@ucsu.Colorado.EDU (FUGATE STACEY ANN) Subject: Calorie Restriction Message-ID: Sender: usenet@cnsnews.Colorado.EDU (Net News Administrator) Nntp-Posting-Host: ucsu.colorado.edu Organization: University of Colorado, Boulder Distribution: usa Date: Sun, 20 Feb 1994 21:00:59 GMT Lines: 8 I'm a molecular biology major doing a paper on calorie restriction and longevity. Does anyone have any information regarding the recent primate studies in this field? Ann fugate@ucsu.colorado.edu From owner-ageing@net.bio.net Mon Feb 21 22:00:00 1994 Path: biosci!headwall.Stanford.EDU!ames!elroy.jpl.nasa.gov!usc!howland.reston.ans.net!nctuccca.edu.tw!news!news.csie.nctu.edu.tw!mjhsieh From: mjhsieh@life.nthu.edu.tw (Meng-Juei Hsieh) Newsgroups: bionet.molbio.ageing Subject: Definition of "Ageing" Date: 22 Feb 1994 15:01:45 GMT Organization: Dep. Computer Sci. & Information Eng., Chiao Tung Univ., Taiwan, R.O.C Lines: 15 Message-ID: <2kd6op$k9s@news.csie.nctu.edu.tw> NNTP-Posting-Host: @life.nthu.edu.tw X-Newsreader: TIN [version 1.2 PL2] Hello netters: Can I ask you all a question ? That is ; "what is the definition of ageing?" Cause I had quarreled with my freinds on this topic for a long long time. -- /////////////////////////////////////////////////////////////////// / Francis M.J. Hsieh 謝孟叡 / / Life Science NTHU ,HsinChu 清大生命科學系九五級 / /Email address: u801629@WinKie.Oz.nthu.edu.tw / / mjhsieh@life.nthu.edu.tw / /////////////////////////////////////////////////////////////////// From owner-ageing@net.bio.net Mon Feb 21 22:00:00 1994 Path: biosci!ESSEX.HSC.COLORADO.EDU!santhosc From: santhosc@ESSEX.HSC.COLORADO.EDU (Santhosh Kumar) Newsgroups: bionet.molbio.ageing Subject: dementia/micronutrients/neurotransmitters Date: 22 Feb 1994 23:53:42 -0000 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Distribution: bionet Message-ID: Reply-To: Santhosh Kumar NNTP-Posting-Host: net.bio.net We are a group of MD biochemists and neurotransmitter chemists at University of Colorado Health Sci Ctr in Denver, who wish to develop collaborative efforts examining the relationship between micronutrient status and biochemical pathways involved in mental health. (folate and ascorbate dependent pathways including oxidized sulfur amino acids) We are interested in HIV associated neurological disease and neurodegenerative disorders in non-HIV patients but other ideas are welcome. Please contact C.R.Santhosh-Kumar -- e-mail shown above or John Deutsch deutschj@essex.hsc.colorado.edu or J. Fred Kolhouse 303-270-8474 From owner-ageing@net.bio.net Tue Feb 22 22:00:00 1994 Newsgroups: bionet.molbio.ageing Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!torn!watserv2.uwaterloo.ca!gobbi From: gobbi@healthy.uwaterloo.ca (gobbi sebastiao) Subject: Re: Definition of "Ageing" Message-ID: Sender: news@watserv2.uwaterloo.ca Organization: University of Waterloo References: <2kd6op$k9s@news.csie.nctu.edu.tw> Date: Wed, 23 Feb 1994 20:21:19 GMT Lines: 10 Personally, I like the definition expressed by King (1988) : Aging is defined as a genetic physiological process associated with morphological and functional changes in cellular and extracellular components aggravated by injury troughout life and resulting in a progressive imbalance of the control regulatory systems of the organism, including hormonal, autocrine, neuroendocrine and immune homeostatic mechanisms. King, D.W. (1988) Pathology and aging. In: Kent, B. & Butler, R. (Eds), Human Aging Research : concepts and techniques, pp.325 - 340, New York, NY: Raven Press. From owner-ageing@net.bio.net Wed Feb 23 22:00:00 1994 Path: biosci!sjubiol.stjohns.edu!rick From: rick@sjubiol.stjohns.edu (Richard Lockshin) Newsgroups: bionet.molbio.ageing Subject: definition of aging Date: 24 Feb 1994 07:23:40 -0000 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9402231513.AA02998@sjubiol.stjohns.edu> NNTP-Posting-Host: net.bio.net To: ageing@net.bio.net From: uunet!life.nthu.edu.tw!mjhsieh (Meng-Juei Hsieh) (Francis MJ Hsieh) Subject: Definition of "Ageing" Can I ask you all a question ? That is ; "what is the definition of ageing?" ->-> I presume that you mean "senescence", which is usually as an increased probability, with time, of dying. Thus, organisms that show a linear disappearance over time are not considered to senesce, but organisms, like mammals, that show a (logarithmically) increasing probability of dying as they age are considered to senesce. See Alex Comfort's classic "The Biology of Senescence," a book with a somewhat similar title by Peter Medawar, the introductory chapters in Finch and Hayflick (1st edition: Finch and others, later editions) Handbook of the Biology of Aging, or Finch (CE)'s latest encyclopedic tome. That should provide enough for you. --richard lockshin From owner-ageing@net.bio.net Fri Feb 25 22:00:00 1994 Path: biosci!bcm!mbcr.bcm.tmc.edu!th035681 From: th035681@mbcr.bcm.tmc.edu (Timothy R. Hughes) Newsgroups: bionet.molbio.ageing Subject: FAQ for aging newsgroup Date: 26 Feb 1994 20:24:40 GMT Organization: Baylor College of Medicine, Houston, Tx Lines: 94 Distribution: world Message-ID: <2kob68$4q1@gazette.bcm.tmc.edu> NNTP-Posting-Host: mbcr.bcm.tmc.edu I have noticed that other newsgroups have monthly FAQ postings (Frequently Asked Questions), which serve to 1.) briefly describe topics encompassed, in order to indicate what sort of postings are appropriate, and what's going on in general, and 2.) provide a list of a few general references on the field on the whole and/or particular topics. As far as I can tell there is no FAQ for the aging newsgroup so unless anyone has any objections I am going to start one. I will keep the file in my account and add to it as I think of things, as people mail me suggestions, and as new publications come out. I will post it around the first of each month. Hopefully this will increase participation and boost the quality of the postings to the aging newsgroup. Judging from the number of people I have spoken with here (I am at Baylor College of Medicine) about the Aging newsgroup, many more people are reading it as are posting to it. For this first FAQ I will post a tentative outline of topics. In subsequent postings I will probably have to modify the outline. Following the outline will be listings under the subject headings in the outline. Under each subject heading I will put a brief description of the subject, and then some general references (review articles or books) and any recent publications. I will have to limit the size of this stuff, since I don't want to get much over 1000 lines, and Finch wrote an 800 page book doing the same thing. If anybody notices something obvious I left out, or has any suggestions or criticism, please let me know. Also if you have a ready-to-go short description for any of the topics, I will try to put it in. My address should accompany this posting but if not mail to: th035681@bcm.tmc.edu and please put "Re: Aging FAQ" in the subject line if possible. AGING FAQ OUTLINE (tentative) (some of these topics overlap) I. Definition of Aging A. Senescence B. Mortality C. Biomarkers of aging in humans 1. prevalent age-related illnesses II. Theories and evidence regarding the aging of multicellular organisms A. Population genetics arguments 1. Antagonistic Plieotropy 2. Longevity Assurance Genes A. DNA Damage 1. random 2. free radicals 3. carcinogens 4. immortality through offspring vs. DNA damage model B. Endocrine cascade 1. growth hormone studies 2. phenotypic changes in endocrine organs C. Cell Senescence 1. telomere shortening 2. vs. cell immortality: cell-cycle related issues F. DNA methylation G. Other III. Aging in model organisms A. Humans 1. progeroid syndromes B. Rodents 1. calorie restriction C. Drosophila 1. selected long-lived mutants 2. engineered mutants D. C. Elegans 1. age mutants E. Other Species F. Relationship of biomarkers of aging in humans to those in model organisms IV. Other interesting phenomena, etc. Please help me out! From owner-ageing@net.bio.net Sun Feb 27 22:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!pipex!warwick!not-for-mail From: cuhes@csv.warwick.ac.uk (Malcolm McMahon) Newsgroups: bionet.molbio.ageing Subject: Re: Definition of "Ageing" Date: 28 Feb 1994 14:17:36 -0000 Organization: University of Warwick, Coventry, UK Lines: 13 Message-ID: <2ksue0$v2@violet.csv.warwick.ac.uk> References: <2kd6op$k9s@news.csie.nctu.edu.tw> NNTP-Posting-Host: violet.csv.warwick.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit In article , gobbi@healthy.uwaterloo.ca (gobbi sebastiao) writes: >Personally, I like the definition expressed by King (1988) : Aging is >defined as a genetic physiological process associated with morphological >and functional changes in cellular and extracellular components >aggravated by injury troughout life and resulting in a progressive >imbalance of the control regulatory systems of the organism, including >hormonal, autocrine, neuroendocrine and immune homeostatic mechanisms. > Wow! Is that a definition, or a theory? Malcolm From owner-ageing@net.bio.net Sun Feb 27 22:00:00 1994 Path: biosci!daresbury!trane.uninett.no!sunic!pipex!zaphod.crihan.fr!jussieu.fr!univ-lyon1.fr!ghost.dsi.unimi.it!batcomputer!news.reed.edu!usenet From: tkim@reed.edu (Tae Hoon Kim) Newsgroups: bionet.molbio.ageing Subject: STUDENT QUESTION: relationship between senescence and apoptosis Date: 28 Feb 1994 23:14:22 GMT Organization: Reed College, Portland, Oregon Lines: 55 Message-ID: <2kttse$3eg@scratchy.reed.edu> NNTP-Posting-Host: reed.edu I am a fourth year undergraduate student working on a senior thesis concerning a possible role of cell death in a mutant phenotype of Arabidopsis thaliana. I am in the process of describing this mutant phenotype and making anatomical observation of the developing leaf. There are some circumstantial evidences which suggest that some of the mutant phenotype are resultant of unregulated(?) cell death. I am interested in getting opinions on the relationship between senescence and apoptosis (or programmed cell death). Kane et al.('93), in Science 262:1274-77, has made a observation that bcl-2 inhibits neural death by decreasing the generation of reactive oxygen species. According to my limited knowledge of this field the increased generation of reactive oxygen species is noted for the senescing cells. In plants, there is a dearth of knowledge concerning apoptosis (or programmed cell death). Much of the studies of plant cell death are addressed in the perspective of cell senescence. Consequently, there are much more information concerning the increased generation of reactive oxygen species in a dying plant cells and tissue. In addition, there are studies that indicate decreased activity of SOD in senescing tissues. My specific questions are the following: 1. Are there evidences that may implicate PKC in the pathwway of cell ageing? 2. Are ultrastructural, morphological, behavior changes in ageing cells similar to apoptotic cells? 3. How is senescence regulated? Or is it regulated at all? 4. How are ageing cells affected by environment? 5. How much evidence are there to suggest that senescence is a genetic process? 6. What is the relationship between senescence and cell cycle? 7. Can this generalization hold?: Senescence occurs in a terminally differentiated cells, while apoptosis occurs in relatively more neoplastic tissues. I would appreciate any comments on the above set of questions. Can I view apoptosis as a suicide and senescence as a natural death? This distinction seems to helpful in conceptualizing these ideas. Thanks your time and patience with my limited English skills. Your responses can be posted to this newsgroup or can be directly e-mailed to me. Thanks again! Tae Hoon Kim tkim@reed.edu Reed College, Box 1140 Portland, OR 97202 From owner-ageing@net.bio.net Mon Feb 28 22:00:00 1994 Path: biosci!NET.BIO.NET!kristoff From: kristoff@NET.BIO.NET (David Kristofferson) Newsgroups: bionet.molbio.ageing Subject: IMPORTANT BIOSCI INFORMATION Date: 1 Mar 1994 10:00:30 -0000 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 244 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199403011000.CAA12350@net.bio.net> NNTP-Posting-Host: net.bio.net Three important items follow: BIOSCI archive searching by e-mail, the BIOSCI FAQ, and the BIOSCI User Address Directory form. If you have not yet listed yourself in our e-mail address directory, please take a few minutes to complete and return the form below. If your address information has changed since you listed yourself, please send us an updated form. Sincerely, Dave Kristofferson BIOSCI/bionet Manager kristoff@net.bio.net **** SEARCHING BIOSCI ARCHIVES WITH WAISMAIL **** E-mail users can search the BIOSCI archives by using our waismail e-mail server. For instructions send the message help to waismail@net.bio.net. Leave the Subject: line blank. Other methods of searching the archives via WAIS and gopher are described in the BIOSCI FAQ. **** BIOSCI FREQUENTLY ASKED QUESTIONS (FAQ) SHEET **** New users of BIOSCI/bionet may want to read the "Frequently Asked Questions" or "FAQ" sheet for BIOSCI. The FAQ provides details on how to participate in these forums and is available for anonymous FTP from net.bio.net [134.172.2.69] in pub/BIOSCI/doc/biosci.FAQ or for retrieval by gopher to net.bio.net, port 70. It may also be requested by sending e-mail to biosci-help@net.bio.net (use plain English for your request). The FAQ is also posted on the first of each month to the newsgroup BIONEWS/bionet.announce immediately following the posting of the BIOSCI information sheet. **** BIOSCI USER ADDRESS DIRECTORY **** Please take this opportunity to add your name and address information to the BIOSCI User Address Database if you have not already done so. Below is the address form that we would like each reader of the BIOSCI/bionet newsgroups to complete and return if you would like to be listed in our database. The database serves as a directory that enables biologists, who are currently using (or even just reading) the BIOSCI newsgroups, to look up e-mail addresses and other information about our users. The address database is reindexed nightly for WAIS and waismail access (waismail is our WAIS e-mail server, more below) and will also be available for access via other gopher sites if they wish to permit it. The raw unindexed data is available for FTP from net.bio.net and is atomized sufficiently to allow import into your local RDBMS should you so desire. Please carefully follow the instructions for completing the form below and return it to either of the following two addresses (whichever is more convenient for you). Thanks in advance for taking the time to complete and return the form. Addresses for returning forms Location Network ----------------------------- -------- ------- biovote@net.bio.net U.S.A. Internet/BITNET biovote@daresbury.ac.uk U.K. JANET MAKING SURE THAT YOUR INFORMATION IS CURRENT This notice will be mailed bimonthly to each newsgroup. You should check our WAIS source or waismail e-mail server from time-to-time to see if your address information is still up-to-date. Send the message help to waismail@net.bio.net for instructions on using waismail. Leave the Subject: line in your message blank. Using Gopher to complete the form --------------------------------- If you don't want to use a text editor, you can also use Dan Jacobson's gopher site to fill out the address database form as follows. Otherwise skip this section on gopher and proceed to the instructions for filling out the form below. > To add yourself to the database just point your > gopher client at merlot.gdb.org and select the following: > > --> 15. Searching For Biologists/ > > --> 9. E-mail Addresses of Biosci-Bionet Users/ > > --> 1. Add (or Correct) Your Address to the BIOSCI User Address > Data.. > > > And fill out the form. or Rob Harper's gopher site in Europe as follows: > Europeans can point their gopher client at gopher.csc.fi and add their > information to the database. All entries will be mailed directly to > Dave for incorporation in a wais source. > > The path to the questionare is as follows. > > ---> 10. Finnish EMBnet BioBox/ > > ---> 8. FAQ Files/ > > FAQ Files > > 1. EMBnet: Information. > 2. EMBnet: Internet resources guide. > 3. A Biologist's Guide to Internet Resources/ > 4. All FAQs (Frequently Asked Questions) Searches and Archives/ > --->5. Bionauts Address Database (questionaire) IMPORTANT INSTRUCTIONS - PLEASE READ CAREFULLY Please enter all responses after the : on each line, leaving one (1) blank space after the : (i.e., before the start of your text). Please do NOT extend your responses past the end of each line (80 characters) or alter any of the field identifiers such as "first name: ". Several lines are provided at the end of the form for comments, but, please adhere to the line length restriction. On the date: line, please enter the date in the DD-MM-YY format, e.g., 05-05-93 for 5 May 1993. This line will tell others when the information was last updated. Please be sure to include the 0's for single digit days or months, e.g., 05-05-93, not 5-5-93. Note that the "e-mail network: " line below is for specifying, e.g., "Internet," "BITNET," "EARN," "JANET," or whatever other network that your computer may be on. If you are uncertain about any field, please feel free to leave it blank, but please DO NOT DELETE the field identifier from the form! In the first field below, "New information or Update ...", please enter "N" if this is the first time that you have registered in the directory or "U" if you are correcting a listing that you sent to us previously. The comment: lines may be used for anything that you like but PLEASE DO NOT DELETE THEM FROM THE FORM OR ALTER THEM. One suggested use is to list the names of the newsgroups in which you participate. Please use the MAILING LIST name (see below - the latest version of the list can be requested from biosci@net.bio.net) instead of the USENET name even if you don't participate by e-mail. WAIS might get confused by the periods in the USENET names. This allows one to retrieve via WAIS or waismail the list of participants in a particular group. For example: comment: ARABIDOPSIS PLANT-BIOLOGY BIONEWS On the comment: lines use these names below ---- NOT the USENET names below MAILING LIST NAME USENET Newsgroup Name ----------------- --------------------- ACEDB-SOFT bionet.software.acedb AGEING bionet.molbio.ageing AGROFORESTRY bionet.agroforestry ARABIDOPSIS bionet.genome.arabidopsis BIOFORUM bionet.general BIO-INFORMATION-THEORY bionet.info-theory BIONAUTS bionet.users.addresses BIONEWS bionet.announce BIO-JOURNALS bionet.journals.contents BIO-MATRIX bionet.molbio.bio-matrix BIO-SOFTWARE bionet.software CHROMOSOMES bionet.genome.chromosomes COMPUTATIONAL-BIOLOGY bionet.biology.computational DROSOPHILA bionet.drosophila EMBL-DATABANK bionet.molbio.embldatabank EMPLOYMENT bionet.jobs GDB bionet.molbio.gdb GENBANK-BB bionet.molbio.genbank GENETIC-LINKAGE bionet.molbio.gene-linkage HIV-MOLECULAR-BIOLOGY bionet.molbio.hiv HUMAN-GENOME-PROGRAM bionet.molbio.genome-program IMMUNOLOGY bionet.immunology INFO-GCG bionet.software.gcg JOURNAL-NOTES bionet.journals.note METHODS-AND-REAGENTS bionet.molbio.methds-reagnts MOLECULAR-EVOLUTION bionet.molbio.evolution NEUROSCIENCE bionet.neuroscience N2-FIXATION bionet.biology.n2-fixation PHOTOSYNTHESIS bionet.photosynthesis PLANT-BIOLOGY bionet.plants POPULATION-BIOLOGY bionet.population-bio PROTEIN-ANALYSIS bionet.molbio.proteins PROTEIN-CRYSTALLOGRAPHY bionet.xtallography RAPD bionet.molbio.rapd SCIENCE-RESOURCES bionet.sci-resources TROPICAL-BIOLOGY bionet.biology.tropical VIROLOGY bionet.virology WOMEN-IN-BIOLOGY bionet.women-in-bio YEAST bionet.molbio.yeast Listing newsgroups on the comment: line is optional, of course. Thanks again for your cooperation! --------------- please cut here and return portion below --------------- New information or Update to old record (enter N or U): date (DD-MM-YY): first name: middle initial: family name: job title: e-mail address: e-mail network: phone number: FAX number: institution: address1: address2: address3: city: state/province: country: postal code: research interest: research interest: comment: comment: comment: comment: comment: