From owner-ageing@net.bio.net Fri Apr 01 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!noc.near.net!news.delphi.com!usenet From: GREGORY C.O'KELLY Newsgroups: bionet.molbio.ageing Subject: molecular biology, cellular senescence,and the lessons of physics Date: Sat, 2 Apr 94 02:56:47 -0500 Organization: Delphi (info@delphi.com email, 800-695-4005 voice) Lines: 15 Message-ID: NNTP-Posting-Host: bos1f.delphi.com The following message is an abstract of a paper that deals with the history of biological thought with regard to the nature of bioelectricity and nerve impulse propagation. Although the paper focuses on neurophysiology, the lessons contained therein are directly relevant to understanding the nature of inter and intracellular chemistry, and allusions are made to these issues in the paper. The point is made that the 'energy' produced by mitochondria and necessary for cellular chemical activity is nothing more than chemical energy wich is known as negative electrical charge. The failure of orthodox medicine to appreciate the lessons of quantum electrodynamics in this regard is ex- tensively detailed. The consequences for the study of cell ageing are signif- icant. By bombarding a cell with electrical charge it is energized as if by powerfully functioning mitochondria. It is not enough to immerse the cell in a medium conducive to its perpetuation and study how it runs down in order to gain an idea of the cell's lifespan. The paper alludes too the nature of the ageing process of the organism itself in terms of its cellular chemistry. From owner-ageing@net.bio.net Sun Apr 03 23:00:00 1994 Path: biosci!agate!ihnp4.ucsd.edu!usc!howland.reston.ans.net!noc.near.net!news.delphi.com!usenet From: GREGORY C.O'KELLY Newsgroups: bionet.molbio.ageing Subject: Re: molecular biology, cellular senescence,and the lessons of ph Date: Sun, 3 Apr 94 21:04:44 -0500 Organization: Delphi (info@delphi.com email, 800-695-4005 voice) Lines: 120 Message-ID: References: NNTP-Posting-Host: delphi.com X-To: GREGORY C.O'KELLY I present an abstract of a paper, "Biology, Bioelectricity, and the Nervous System", which I would like to have considered by others of a scientific bent. The paper is at once challenging and soporific. It is challenging because the material is quite cross disciplinary, and involves the history and the philosophy of science as well as knowledge about paleoanthropology, medicine, evolutionary theory, and physics. It is soporific because it is often technical or esoteric, so that few will grasp its entirety, and many will set it aside unfinished. The paper begins with a discussion of the attempt by biologists, most notably Ernst Mayr of Harvard, to qualify biology as scientific by specifying what qualifies as science. The paper points out that even by this specification biology does not qualify as science, and that the Mayrian notion that biology has escaped the millstone of Descartes is incorrect. The paper points out how Mayr's own biological prejudices against the notion of discontinuity in vertebrate and mammalian speciation flow from his desire to maintain the barrier between biology and physics. This issue is returned to in the epilogue. It is one of the theses of this paper that Descartes and Newton still exercise a strong influence over biological thought, especially in the idea that only the first of the four fundamental forces of nature (gravity, electromagnetism, the strong force, the weak force) is considered pertinent to biology, that creatures are thought of as mechanisms, and that is what biochemists look for. It is pointed out that this outlook is especially characteristic of thinking about the nervous system, the lack of a model of the functional organization of that system still being what Mayr calls a bottleneck in biological thought. In the first part of the paper, NEUROLOGY, ELECTRICITY AND THE NATURE OF NERVE IMPULSE PROPAGATION, a history of medical electricity is presented simultaneously with a discussion of how the thinking of physicists was changing with regard to the nature of electricity. It is pointed out that most of the conclusions with regard to the use of electrical stimulation to simulate nervous functioning were made before the electron was even hypothesized, the rest were made before the nature of the electron was understood by physicists. In this section is a discussion of the galvanic/faradic distinction - that is, the difference between AC and DC in terms of electron behavior, and how medicine failed to grasp the significance of polarity of electrical charge on chemical reactions, especially those involving neurotransmitters. The next section, VIEWS OF NERVOUS SYSTEM FUNCTIONING, starts with a discussion of how medical/neurological views of nervous functioning and electricity solidified in 1870, long before the physicists understood the nature of electricity. These ideas were institutionalized in the name of Sir Charles Sherrington, the father of modern neuro-physiology, in the first part of this century. It is stated that even Peter Medawar observes as recently as 1983 that neurology can do nothing more than it could do 100 years earlier. Thorough discussion of the role of the ignorance of electricity's functioning upon 19th century speculation about nervous functioning is presented. It is shown how the structure of neuroanatomy was misinterpreted in its functioning prior to and in the first part of the 20th century because the working of electricity was not yet even understood by physicists, even though it was already being used by engineers. It is pointed out in this section that traditional explanations of nervous functioning were never questioned, and were even awarded a Nobel Prize in 1963 for a bit of work by Sir John Eccles, Hodgkin and Huxley that is an affront to all philosophers of science and physicists, but is still taught as doctrine today. Neurologists insist on retaining a view of the nervous system that leaves it in an impoverished state with regard to possibly being an information processing system, and neuroscientists no longer question sciosophistic explanations of nerve impulse propagation that leave their discipline the most inept branch of clinical medicine. In the next section, THE FUNCTIONING OF THE NERVOUS SYSTEM BASED UPON AN EXAMINATION OF ITS STRUCTURE AND CONSIDERATION OF THE NATURE OF BIOELECTRICITY, the effects on organic molecules and the origins of life, and on extra and intracellular chemistry of electrical charge, are detailed. This is especially crucial for understanding motor functioning, and for seeing 'behavior' as the result of the movement of electrical charge, not the intervention of mind and consciousness in a dualistic world. For the biologists and their mechanisms, 'energy' and 'information' meant molecular momentum and some anthropomorphic form of cellular intelligence. For the physicists it meant direct current and electrolysis. In this section is also discussed the electronmicroscopic structure of muscle and how it legislates against Sherrington's own 'reflex activity of the spinal cord', reflexes being something that every neurologist knows how to check for, and how useless such things are for diagnosis and treatment. In addition, parallels between atrophy and aging are discussed as well as the possibility that some paralysis resulting from CNS trauma are do to deterioration of the muscle and not irreversible CNS damage. In the next section, NERVOUS SYSTEM FUNCTIONING, COMPLEXITY, AND STIMULATION, the relation between motor activity and brain complexity is clearly staked out. In addition, organism behavior as well as the functioning of the organism's cells are hypothesized to be dependent upon the same laws of nature, the movement of electrochemical energy, rather than the cells being electrochemical while the organism has a consciousness or some 'vital' force within it. With this in mind it is detailed how the CNS and the body may be maintained by the introduction of electrical charge via transcutaneous electrode, thereby preventing or curing chronic and degenerative illnesses and aging. Finally, in the epilogue it is discussed once again how Mayr's views of the continuity of genetic change and vertebrate speciation conflict not only with the fossil record (which is more supportive of the views of Gould and Eldridge with their 'punctuated equilibria'), but with a view of the nervous system which holds that nervous system complexity is better indicated by the number of emergent axons from that system, whether centrally or peripherally, than relative brain and body weights and cranial capacities. The number of axons is associated with the ganglia and alpha motor neurons, with the addition of pairs of these due to 'systemic mutations', an idea expressly disdained by Mayr. It is stated, in conclusion, that this approach to the nervous system is right in line with the calls of Gerald Edelman, Nobel Laureate and director of the Neurosciences Institute and chairman of the Department of Neurobiology at the Scripps Research Institute, calls for 'a theory of how morphology arises and how it is changed during evolution'; 'how alterations in form, either in the whole animal or at microscopic levels of brain, evolution; Mayr calls it 'macroevolution'. I would greatly appreciate the comments and criticisms of anyone who has the patience and perseverance to finish this paper and to ruminate over the ideas presented therein. This is just the proffered foundation of a theory, an hypothesis which can overturn its competition from the orthodox neurological community by resort to electronmicroscopic muscle biopsies which will overrule the clinical manual of neuroanatomy, flawed as it is, with regard to spinal reflexes being posited as responsible for the spasticity and rigidity of the spinal injured. I would like someday, having gotten this considered, to answer the 'so what?' of the issue as it relates to clinical application to chronic and degenerative illnesses and the aging process. For a copy of this paper binhexed please write to gokelly@delphi.com From owner-ageing@net.bio.net Mon Apr 04 23:00:00 1994 Path: biosci!CSUVAX1.MURDOCH.EDU.AU!sutarno From: sutarno@CSUVAX1.MURDOCH.EDU.AU (Sutarno) Newsgroups: bionet.molbio.ageing Subject: new subscriber Date: 4 Apr 1994 20:55:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: bionet Message-ID: NNTP-Posting-Host: net.bio.net I would like to subscribe to the net. My complete name and address are: Nano Sutarno Vet. Biology Vet. School Murdoch University WA 6150 AUSTRALIA From owner-ageing@net.bio.net Wed Apr 06 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!swrinde!emory!news.cc.emory.edu!wzhou-sl.cc.emory.edu!wzhou From: wzhou@emoryu1.cc.emory.edu Newsgroups: bionet.molbio.ageing Subject: Apoptosis and Necrosis Date: 7 Apr 1994 14:53:10 -0400 Organization: Emory University Lines: 7 Sender: news@emoryu1.cc.emory.edu Message-ID: NNTP-Posting-Host: emoryu1.cc.emory.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev B] Dear Netters: What is the single most important distinctions between these two kinds of cell death? I am puzzled by the traditional classifications. Few days ago, I post a message asking whether apoptotic bodies would exclude trypan blue. I got only one response. Do apoptotic cells maintain their membrane intergrity? wzhou@bimcore.cc.emory.edu From owner-ageing@net.bio.net Thu Apr 07 23:00:00 1994 Path: biosci!sjubiol.stjohns.edu!rick From: rick@sjubiol.stjohns.edu (Richard Lockshin) Newsgroups: bionet.molbio.ageing Subject: apoptosis (speakers) Date: 7 Apr 1994 20:39:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9404072226.AA20504@sjubiol.stjohns.edu> NNTP-Posting-Host: net.bio.net I am beginning to organize a conference on cell death (scheduled for August, 1995). I have a potential program in mind but, in order to do the best job that I can, I would be interested to hear other's thoughts on topics or speakers. I would appreciate a line or two of justification for topics. In terms of speakers, the operative adjectives would be: thoughtful, provocative, creative, insightful, analytical. Please reply via private email to rick@sjubiol.stjohns.edu. I give a report later on as appropriate. From owner-ageing@net.bio.net Thu Apr 07 23:00:00 1994 Path: biosci!sjubiol.stjohns.edu!rick From: rick@sjubiol.stjohns.edu (Richard Lockshin) Newsgroups: bionet.molbio.ageing Subject: apoptosis and necrosis Date: 7 Apr 1994 22:50:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9404080458.AA20647@sjubiol.stjohns.edu> NNTP-Posting-Host: net.bio.net mzhou@bimcore.cc.emory.edu asks for the distinctions between apoptosis and necrosis. start with the Tomei-Cope book (Cold Spring Harbor Press) and Wyllie's review 2 years ago. Otherwise, see the review by Zakeri and Lockshin in the Ann NY Acad Sci edited by Franceschi C et al last year. Necrosis is usually described as an uncontrolled membrane-failure lysis, resulting in swelling and rupture of the cell and organelles and (in mammals) an inflammatory response. Apoptotic cells typically shrink, apparently retaining membrane integrity (though one or a few authors dispute the point) and show nuclear condensation and at least temporary preservation of organelle structure. there are both lysosomal and non- lysosomal forms of controlled cell death. the trypan blue staining is typically for gross identification and is usually for macrophages that are destroying these apoptotic bodies. in vivo, apoptotic bodies exist for very short periods (Schulte-Hermann and Bursch, about 3 years ago, or see the Cold Spring Harbor book for an article by Schulte-Hermann). Apoptotic bodies can be identified by electron microscopy, H & E or toluidine blue staining, or in situ end-labeling (tunnel technique, Apoptag kit (Oncor)). Free-floating cells of reticulo-endothelial origin may be recognized by fluorescence-sorting techniques, looking for DNA content < 2N (numerous authors, including Wyllie and S. Umansky). the programmed cell death paradigm, requiring synthesis of apparently new proteins, is not easy to detect at present, since no unique protein can be claimed to document apoptosis. (proteins such as TRPM-2 and --correction, TRPM-2, alias clusterin or SGP-2--myc, fos, or ubiquitin) are clearly NOT "cell death proteins". High levels of cross-linkage may be useful but the technique is difficult. See also Zakeri et al, FASEB J January 93; a follow-up is coming. If you have a specific instance in mind, I can make a more effective suggestion. From owner-ageing@net.bio.net Fri Apr 08 23:00:00 1994 Path: biosci!sjubiol.stjohns.edu!rick From: rick@sjubiol.stjohns.edu (Richard Lockshin) Newsgroups: bionet.molbio.ageing Subject: apoptosis and necrosis Date: 8 Apr 1994 20:39:52 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9404081534.AA20891@sjubiol.stjohns.edu> NNTP-Posting-Host: net.bio.net Subject: Re: apoptosis and necrosis Newsgroups: bionet.molbio.ageing Tae H. Kim tkim@reed.edu writes >Regarding lysosomal and non-lysosomal PCD, I would like know if >these two serve different functions in regards to development >and physiology. I would appreciate any references that you might have concerning these two pathways(?) >of PCD. see the following: Schweichel, J. U. and Merker, H. J. (1973) The morphology of various types of cell death in prenatal tissues. Teratology. 7, 253-266. Clarke, P. G. H. (1990) Developmental cell death: Morphological diversity and multiple mechanisms. Anat. Embryol. 181, 195-213. Beaulaton J and Lockshin RA (1982) The relation of programmed cell death to development and reproduction: comparative studies and an attempt at classification. Int. Rev. Cytol. 29: 215-235. a group of us are developing a paper to bring back to attention these forgotten papers. There is no particular theory, except to note that cell debris is consumed either by intracellular lysosomes (autophagic vacuoles) or by vacuoles in other cells (phagocytosis). The former is commonly seen in insects and in tissues such as the mammary gland, but other than the papers listed above it hasn't been well worked out. From owner-ageing@net.bio.net Sun Apr 10 23:00:00 1994 Path: biosci!agate!ihnp4.ucsd.edu!swrinde!emory!news.cc.emory.edu!wzhou-sl.cc.emory.edu!wzhou From: wzhou@emoryu1.cc.emory.edu Newsgroups: bionet.molbio.ageing Subject: More about Apoptosis Date: 11 Apr 1994 13:21:37 -0400 Organization: Emory University Lines: 33 Sender: news@emoryu1.cc.emory.edu Message-ID: NNTP-Posting-Host: emoryu1.cc.emory.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev B] Thank you all for your response to my previous post seeking your opinion about the distinctions between apoptosis and necrosis. I asked "Is it true that apoptotic cells maintain their membrane intergrity?" Most people said "yes". Some people said, specifically, that apoptotic cells exclude trypan blue. But, many published papers, especially those demonstrating the protective effect of bcl-2, used trypan blue exclusion as a measurement of cell death. Are those types of cell death really apoptotic or should they be considered as necrotic? In normal intestinal cell lines, I can detect DNA fragmentation. I call it spontaneous cell death and it is somehow cell density dependent. I can see very few trypan blue stained cells even though I can identify and then purify detergent-insoulbe envelope (apoptotic bodies). I assumed apoptotic cells were engulfed by neigboring cells before they lost their membrane intergrity. In some transformed cell lines, such as HT-29, most dead cells detach from the monolayer. Detached cells can be judged as apoptotic by acridine organe staining and are all stained well with trypan blue, even the cells maintaining good morphology (I assume they are in the early stage of cell death) . I got beautiful DNA ladder from those cells. Is it possible that apoptotic cells, when not engulfed by other cells, can not maintain their membrane intergrity? Also, what do you think is the function of apoptotic bodies? I would appreciate your comments. Wei Zhou Emory University wzhou@bimcore.cc.emory.edu From owner-ageing@net.bio.net Mon Apr 11 23:00:00 1994 Path: biosci!sjubiol.stjohns.edu!rick From: rick@sjubiol.stjohns.edu (Richard Lockshin) Newsgroups: bionet.molbio.ageing Subject: apoptosis and necrosis Date: 11 Apr 1994 20:40:30 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 54 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9404111456.AA22161@sjubiol.stjohns.edu> NNTP-Posting-Host: net.bio.net To: rick@sjubiol.stjohns.edu From: uunet!reed.edu!tkim (Tae Hoon Kim) Subject: apoptosis and necrosis Dear Dr. Lockshin, >Thank you for the references. I was able to locate your article in our >library--other two couldn't be found in my school library. The article was >very helpful for me. It seems that "autophagic degeneration" occurs in >plants as a form of programmed cell death. > >Do you suggest that there are more varied forms of necrosis and these can >be "programmed"? My initial understanding of the term was that necrosis >referred to accidental and "unprogrammed" death resulting from trauma or >stress, thus making necrosis non-physiological death. Is it correct in >stating that necrosis is a descriptive term, just as apoptosis is? If >programmed cell death is understood as a functional term, then both >necrosis and apoptosis can be understood as "programmed cell death" in >certain contexts? AFTER SOME TIME OF FIGHTING FOR CLEAR USE OF THE TERMS, I HAVE DECIDED THAT THE WORLD WILL COME TO A DEFINITION OF THE TERMS WITH OR WITHOUT MY EFFORTS TO CONTROL IT. PROGRAMMING IMPLIES IMMEDIATE GENETIC CONTROL OF THE COLLAPSE, THOUGH NOT NECESSARILY THE PRODUCTION OF DEATH GENES. I'M NOT SURE HOW A NECROTIC DEATH, WHICH IS CONSIDERED TO BE UNCONTROLLED, WOULD FIT THIS DEFINITION, THOUGH SOME METAMORPHIC PROGRAMMED DEATHS MAY RESULT IN OSMOTIC LYSIS. IF THIS IS WHAT YOU MEAN, OK, BUT IT IS VERY UNCOMMON. >I have been reviewing some literature on senescence regarding cell death. >How should I understand senenscence to mean, in terms of cell death. Some >researchers have used the term "programmed senescence" in reference to >plant cell death. I am still confused by these terms, senescence and >programmed cell death. Many of older literature mention cell death in >regards to senescence, but these obeservations of cell death seem to be in >the context of development. > >It seems that even coelenterates undergo programmed cell death. I have not >been able to locate a solid information concerning stalk cell >different. PLANTS AND LOWER INVERTEBRATES DO MANY CRAZY THINGS. FOR INSTANCE, MATURATION OF COTYLEDONS MAY BE A PROGRAMMED DEATH, AND FLOWERS, IN THE TERMS OF PLANT PHYSIOLOGISTS, 'SENESCE', AS DOES THE WHOLE PLANT IN AN ANNUAL. THERE WAS A SMALL LITERATURE A WHILE BACK ABOUT THE DEATH OF CELLS IN COELENTERATES (HYDRA), IN WHICH CELLS FORM AT OR NEAR THE MOUTH, GRADUALLY GET PUSHED TOWARD THE BASE OR FOOT, AND DIE AND ARE DISCARDED THERE. THE TERMS WERE FAIRLY CASUALLY AND SLOPPILY USED, AND MOST OF THE WORK DIED OUT AS FUNDING FOR SUCH PROJECTS DRIED UP. IF YOU COME ACROSS SOME REASONABLY MODERN, THOUGHTFUL WORK ON PLANTS OR LOWER INVERTEBRATES, I'D LOVE TO KNOW ABOUT IT. --richard a lockshin From owner-ageing@net.bio.net Mon Apr 11 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!wupost!ukma!seqanal.mi.uky.edu!mpm From: mpm@seqanal.mi.uky.edu (Mark Mattson) Newsgroups: bionet.molbio.ageing Subject: Apoptotic vs. necrotic Date: 12 Apr 1994 14:27:39 GMT Organization: University of Kentucky, Dept. of Math Sciences Lines: 26 Message-ID: <2oeb4r$rb0@s.ms.uky.edu> NNTP-Posting-Host: seqanal.mi.uky.edu X-Newsreader: TIN [version 1.1 PL9] It seems to me that -- although they may be ultimately different in mechanism of killing -- way too much emphasis has been placed on drawing a distinction between the events which TRIGGER apoptotic or necrotic deaths. I was particulary struck by the futility in this argument at last year's Society for Neuroscience meeting where I saw at least half a dozen posters lined up next to each other, alternating in their conclusions about whether glutamate excitotoxicity triggered necrotic or apoptotic death. It is my opinion that the SAME THING triggers both: FREE RADICALS. Obviously, free radicals can cause the kind of membrane damage characteristic of necrosis, and more and more evidence suggests that free radicals can trigger apoptosis. Ex: bcl-2 seems to prevent apoptosis by antagonizing a radical-evoked event; several labs suggest that tumor necrosis factors (which, despite their name, can trigger apoptosis) use reactive oxygen species in their signal transduction pathways. To me, the distinction seems to be a quantitative one: if free radicals are generated in large amounts, membranes are rapidly damaged; if the levels of oxidation are lower, some program (perhaps, genetic in nature) is activated which kills the cell more slowly. This is consistent with the first descriptions of apoptosis in the liver, where it was caused by the same insult as the coincident necrosis -- the necrosis was in the center of the lesion and the apoptosis was on the periphery. One can easily imagine that this spatial delineation was caused by the decreasing concen- trations of radicals as they diffused concentrically from the center of the lesion. Steven W. Barger, Ph.D. Sanders-Brown Center on Aging From owner-ageing@net.bio.net Tue Apr 12 23:00:00 1994 Path: biosci!sjubiol.stjohns.edu!rick From: rick@sjubiol.stjohns.edu (Richard Lockshin) Newsgroups: bionet.molbio.ageing Subject: More about apoptosis Date: 12 Apr 1994 20:40:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 44 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9404121634.AA22576@sjubiol.stjohns.edu> NNTP-Posting-Host: net.bio.net To: ageing@net.bio.net From: uunet!emoryu1.cc.emory.edu!wzhou Subject: More about Apoptosis >In normal intestinal cell lines, I can detect DNA fragmentation. >I call it spontaneous cell death and it is somehow cell density >dependent. I can see very few trypan blue stained cells even >though I can identify and then purify detergent-insoulbe envelope (apoptotic bodies). I assumed apoptotic cells were engulfed by >neigboring cells before they lost their membrane intergrity. intestinal cells are normally discarded into the lumen rather than being phagocytosed. I assume that you are seeing an (interesting) reflection of this in vitro. Also, cultured cells are not normally phagocytosed. This may be an artifact of the culture system. >In some transformed cell lines, such as HT-29, most dead cells >detach from the monolayer. Detached cells can be judged as >apoptotic by acridine organe staining and are all stained well > with trypan blue, even the cells maintaining good morphology >(I assume they are in the early stage of cell death) . I got >beautiful DNA ladder from those cells. that's nice to hear! >Is it possible that apoptotic cells, when not engulfed by other >cells, can not maintain their membrane intergrity? That would appear to be reasonable. At some point they must not be able to keep up their function. We have no reason to feel that apoptotic bodies are like spores or other dormant cells, and we know of no reversal from the collapsed state. >Also, what do you think is the function of apoptotic bodies? I don't know what you mean by the question. The evolutionary sig- nificance of apoptosis appears to be controlled, non-inflammatory elimination of injured cells, especially in the sense that they are potentially dangerous (carriers of viruses or toxins, or lytic enzymes). The apoptotic body appears to be an encapsulated form of the cell. From owner-ageing@net.bio.net Wed Apr 13 23:00:00 1994 Path: biosci!sjubiol.stjohns.edu!rick From: rick@sjubiol.stjohns.edu (Richard Lockshin) Newsgroups: bionet.molbio.ageing Subject: more on apoptosis/necrosis Date: 13 Apr 1994 20:22:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 61 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <9404131347.AA22937@sjubiol.stjohns.edu> NNTP-Posting-Host: net.bio.net Message 5: From uunet!net.bio.net!BIOSCI-REQUEST Tue Apr 12 23:43:26 1994 Return-Path: Received: from uunet.UUCP by sjubiol.stjohns.edu (4.0/SMI-4.0) id AA22767; Tue, 12 Apr 94 23:43:25 EDT Received: from net.bio.net by relay1.UU.NET with SMTP (5.61/UUNET-internet-primary) id AAwlhz10138; Tue, 12 Apr 94 10:53:04 -0 400 Received: (from daemon@localhost) by net.bio.net (8.6.8.1/8.6.6) id HAA18286 for ageing-list; Tue, 12 Apr 1994 07:28:29 -0700 Received: (from news@localhost) by net.bio.net (8.6.8.1/8.6.6) id HAA18282 for a geing-arpanet; Tue, 12 Apr 1994 07:28:28 -0700 To: ageing@net.bio.net From: uunet!seqanal.mi.uky.edu!mpm (Mark Mattson) Subject: Apoptotic vs. necrotic Date: 12 Apr 1994 14:27:39 GMT Message-Id: <2oeb4r$rb0@s.ms.uky.edu> Nntp-Posting-Host: seqanal.mi.uky.edu X-Newsreader: TIN [version 1.1 PL9] Status: R It seems to me that -- although they may be ultimately different in >mechanism of killing -- way too much emphasis has been placed on drawing >a distinction between the events which TRIGGER apoptotic or necrotic >deaths. I was particulary struck by the futility in this argument at >last year's Society for Neuroscience meeting where I saw at least half a dozen >posters lined up next to each other, alternating in their conclusions about >whether glutamate excitotoxicity triggered necrotic or apoptotic death. >It is my opinion that the SAME THING triggers both: FREE RADICALS. >Obviously, free radicals can cause the kind of membrane damage characteristic >of necrosis, and more and more evidence suggests that free radicals can >trigger apoptosis. Ex: bcl-2 seems to prevent apoptosis by antagonizing a >radical-evoked event; several labs suggest that tumor necrosis factors (which, >despite their name, can trigger apoptosis) use reactive oxygen species >in their signal transduction pathways. To me, the distinction seems to be a >quantitative one: if free radicals are generated in large amounts, membranes >are rapidly damaged; if the levels of oxidation are lower, some program >(perhaps, genetic in nature) is activated which kills the cell more slowly. >This is consistent with the first descriptions of apoptosis in the liver, >where it was caused by the same insult as the coincident necrosis -- >the necrosis was in the center of the lesion and the apoptosis was on the >periphery. One can easily imagine that this spatial delineation was caused >by the decreasing concentrations of radicals as they diffused >concentrically from the center of the lesion. > >Steven W. Barger, Ph.D. >Sanders-Brown Center on Aging you won't get an argument from me on this. You are following pretty much the same argument as Amedeo Columbano and Vanna Ledda-Columbano of Cagliari (Sardinia) Italy. They publish on hepatotoxins, usually in Am. J. Pathol. or similar journals. I think that the image of a lot of people is coalescing: that if a cell has time to think about its injury and nurse its wounds, it is capable of collapsing by an apoptotic means. Otherwise, it lyses (necrosis). The Columbanos observed that moderate-dose hepato- toxins would produce prompt necrosis followed later by a wave of apoptosis. Free radicals are certainly one means of creating cell injury. They may not be the only way. --Richard Lockshin St. John's University From owner-ageing@net.bio.net Wed Apr 13 23:00:00 1994 Path: biosci!ESSEX.HSC.COLORADO.EDU!deutschj From: deutschj@ESSEX.HSC.COLORADO.EDU (John Deutsch) Newsgroups: bionet.molbio.ageing Subject: antioxidants Date: 14 Apr 1994 14:55:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: bionet Message-ID: NNTP-Posting-Host: net.bio.net I am measuring labile nutrient antioxidants in serum, urine and CSF. I am particularly interested in samples from aged or ascorbate deficient guinea pigs. If anyone has an interest in this area or a bank of samples preferably stored below -70, with controls, please contact me. From owner-ageing@net.bio.net Thu Apr 14 23:00:00 1994 Newsgroups: bionet.molbio.ageing Path: biosci!headwall.Stanford.EDU!ames!sgiblab!swrinde!emory!europa.eng.gtefsd.com!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!news.mr.med.ge.com!wind!hinz From: hinz@wind (David Hinz W-641 548-3221 ) Subject: Aging rates/human vs. dog Message-ID: <1994Apr14.235840.7253@mr.med.ge.com> Sender: news@mr.med.ge.com Nntp-Posting-Host: wind Organization: GE Medical Systems, Magnetic Resonance X-Newsreader: TIN [version 1.1 PL8] Date: Thu, 14 Apr 94 23:58:40 GMT Lines: 12 I've been wondering this for a while, and was hoping someone here could answer this: Why do dogs often have cataracts when they reach the age of, say, 12 years, but in humans it takes maybe 5 times as long? Are the cells aging at a different rate, and if so, why? If we knew why, could we do something about affecting aging rates? Thanks - I hope someone can answer what is probably a rather naive and basic question. Dave Hinz From owner-ageing@net.bio.net Thu Apr 14 23:00:00 1994 Newsgroups: bionet.molbio.ageing Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.intercon.com!news.pipeline.com!malgudi.oar.net!sun!oucsace!ouvaxa.cats.ohiou.edu!JANAPATI From: janapati@ouvaxa.cats.ohiou.edu Subject: need post-doc position Message-ID: Sender: news@oucsace.cs.ohiou.edu (news account) Reply-To: janapati@ouvaxa.cats.ohiou.edu Organization: Ohio University Computing & Technology Services Date: Fri, 15 Apr 1994 18:22:27 GMT Lines: 2 seeking post-doctoral opportunity in the area of ageing/neurodegeneration/apoptosis. available immediately. cv will be sent. janapati@ouvaxa.cats.ohiou.edu. From owner-ageing@net.bio.net Thu Apr 14 23:00:00 1994 Path: biosci!VTVM1.CC.VT.EDU!VERPHELD From: VERPHELD@VTVM1.CC.VT.EDU Newsgroups: bionet.molbio.ageing Subject: fas gene Date: 15 Apr 1994 14:20:27 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199404152120.OAA26594@net.bio.net> NNTP-Posting-Host: net.bio.net Autoimmune prone MRL/lpr mice have been reported to have deffective Fas genes which would presumably alter the negative selection process in the thymus. I would like to know if anybody has any thoughtson why this deffective fas gene w ould alter T cell development and yet have no effect on other developmental processes mediatred through apoptosis. ************************************************************************ Daniela I. Verthelyi Virgina Tech. USA VERPHERLD@VTVM1 From owner-ageing@net.bio.net Thu Apr 14 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.ans.net!hp81.prod.aol.net!search01.news.aol.com!not-for-mail From: capital100@aol.com (Capital100) Newsgroups: bionet.molbio.ageing Subject: Anti-aging drugs... Date: 15 Apr 1994 16:31:07 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 5 Sender: news@search01.news.aol.com Message-ID: <2omtib$jn5@search01.news.aol.com> NNTP-Posting-Host: search01.news.aol.com A number of people have queried me in reference to the FDA import guidlines, and availability of anti-oxidants, anti-aging drugs, and "smart drugs" that are not available in the United States. There are several companies that I have dealt with, if interested I would recommend contacting: Life Extension Services, 558-A Baltimore Pike, Suite 230, Bel Air, MD 21014. From owner-ageing@net.bio.net Fri Apr 15 23:00:00 1994 Path: biosci!parc!barrnet.net!well!mjbrauer From: mjbrauer@well.sf.ca.us (Matthew J. Brauer) Newsgroups: bionet.molbio.ageing Subject: Re: fas gene Date: 16 Apr 1994 07:53:35 GMT Organization: The Whole Earth 'Lectronic Link, Sausalito, CA Lines: 21 Distribution: bionet Message-ID: <2oo5hv$cp2@nkosi.well.com> References: <199404152120.OAA26594@net.bio.net> NNTP-Posting-Host: well.sf.ca.us X-Newsreader: NN version 6.5.0 #1 (NOV) VERPHELD@VTVM1.CC.VT.EDU writes: >Autoimmune prone MRL/lpr mice have been reported to have deffective Fas genes >which would presumably alter the negative selection process in the thymus. I >would like to know if anybody has any thoughtson why this deffective fas gene w >ould alter T cell development and yet have no effect on other developmental >processes mediatred through apoptosis. >************************************************************************ > Daniela I. Verthelyi > Virgina Tech. > USA > VERPHERLD@VTVM1 Induction of apoptosis probably proceeds through any of several pathways, of which fas-mediated apoptosis is only one. Many of the downstream apoptosis genes (e.g. bcl-2, bax, etc.) show tissue-specific expression. It's conceivable that the signalling pathway in developing lymphocytes is more finely modulated than in other tissues. Matthew J. Brauer From owner-ageing@net.bio.net Fri Apr 15 23:00:00 1994 Path: biosci!HAL.HAHNEMANN.EDU!springerj From: springerj@HAL.HAHNEMANN.EDU Newsgroups: bionet.molbio.ageing Subject: bcl-2 cDNA Date: 16 Apr 1994 07:25:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <0097D09B.A91D7F60.15@hal.hahnemann.edu> NNTP-Posting-Host: net.bio.net I am interested in obtaining the cDNA for the bcl-2 gene for use in studying amy otrophic lateral sclerosis (ALS). Please send info to my e-mail address: springe rj@hal.hahnemann.edu. Thanks Joe e. Springer, Department of Neurology Hahnemann University Philadelphia, PA 19102 From owner-ageing@net.bio.net Sat Apr 16 23:00:00 1994 Newsgroups: bionet.molbio.ageing Path: biosci!agate!dog.ee.lbl.gov!ihnp4.ucsd.edu!usc!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!msuinfo!netnews.upenn.edu!news.drexel.edu!dunx1.ocs.drexel.edu!st93q84w From: st93q84w@Dunx1.OCS.Drexel.Edu (David Hallowell) Subject: CALL FOR PAPERS IN BIOLOGY Message-ID: Organization: Drexel University X-Newsreader: TIN [version 1.2 PL2] Date: Sun, 17 Apr 1994 03:45:09 GMT Lines: 37 =============================== CALL FOR UNDERGRADUATE PAPERS ---------- [] [] [] THE DREXEL UNIVERSITY JOURNAL OF UNDERGRADUATE [] [] [] [] [] [] [] [] [] BIOLOGICAL RESEARCH [] [] [] [] [] [] Requirements: [] Papers are limited to topics in biology. [] You must be an undergraduate at least assisting with the research on which you plan to write. [] You must use the American Psychological Association (APA) format of citing literature. [see American Scientist for guide]. Due: All papers are due by May 5. If possible, submit papers in MacWrite II compatible format. Submitted materials cannot be returned. :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::| FOR MORE INFORMATION OR TO SUBMIT A PAPER |::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: [] write Journal of Undergraduate Research at The Journal of Undergraduate Research Department of Bioscience and Biotechnology Drexel University 32nd and Chestnut Streets Philadelphia, Pennsylvania 19104 [] send e-mail to martinpf@dunx1.ocs.drexel.edu :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: From owner-ageing@net.bio.net Sun Apr 17 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!news.intercon.com!psinntp!psinntp!psinntp!sjuvm!yprlbio Nntp-Posting-Host: 149.68.2.20 Date: Sat, 16 Apr 1994 12:31:58 -0400 From: "LOCKSHIN, RICHARD A" Newsgroups: bionet.general,bionet.cellbiol,bionet.molbio.ageing Subject: Re: Apoptosis vs. necrosis Message-ID: <16APR94.13517670.0023@sjumusic.stjohns.edu> References: <199404131655.JAA01867@net.bio.net> <1994Apr13.185159.1758@alw.nih.gov> Sender: usenet@sjumusic.stjohns.edu Organization: St. John's University Lines: 48 Xref: biosci bionet.general:8637 bionet.cellbiol:456 bionet.molbio.ageing:715 In article <1994Apr13.185159.1758@alw.nih.gov> bernard@elsie.nci.nih.gov (Bernard Murray) writes: >In article <199404131655.JAA01867@net.bio.net>, CGE@CU.NIH.GOV writes: >> The definition from Dorland's medical Dictionary: >> >> Apoptosis is derived from the Greek Apo- [meaning separation >> or derivation from] and -ptosis [silent p, meaning downward >> displacement]. Apoptosis = a falling off. >> >> Dale, what is so strange sounding about apo-tosis? There are >> many 'pt' words with a silent p - pteridine, pterygoid, Ptolemy, >> asymptote, pteridophyte, ptarmigan, ptomaine, and every kids >> favorite - pterodactyl. >> >> Graham > > >Andrew Wyllie pronounces it as per the Greek derivation (silent p) and I assume >he has some say in the matter. However, since many people over here can't >even pronounce his name correctly I think this is a lost cause. > Personally, I'm no purist and enjoy the ay-POP-toh-siss (said with a >distinct Southern drawl) that seems to be the norm around here. Also, at the >FEBS meeting in Dublin in 1992 another derivation of the name was put forward >which supports the non-silent P.... > >Spontaneously dying cell resembles Popcorn >When encountered, flasks of dying cells are usually tossed in the bin/trash > >so... A "pop"! Toss it! > >(My apologies to the original author of this who had the advantage of a set > of slides to put the point across) > Bernard > > >Bernard Murray, Ph.D. >bernard@elsie.nci.nih.gov (National Cancer Institute, NIH, Bethesda MD, USA) > >. 11 thanks for the note. most of the discussion of apoptosis is taking place in bionet.molbio.ageing (ageing@net.bio.net). I was interested in getting it going in bionet.cellbiol (cellbiol@net.bio.net) but not too many people are interested there. i personally would prefer that it not be in bionet.general--it's too slow to get through it! richard a lockshin, st. john's u (yprlbio@sjumusic.stjohns.edu for news board type of material, or rick@sjubiol.stjohns.edu for correspondence) From owner-ageing@net.bio.net Sun Apr 17 23:00:00 1994 Path: biosci!agate!overload.lbl.gov!dog.ee.lbl.gov!ihnp4.ucsd.edu!swrinde!emory!europa.eng.gtefsd.com!howland.reston.ans.net!noc.near.net!das-news.harvard.edu!husc-news.harvard.edu!husc.harvard.edu!husc9.harvard.edu!gwmartin Newsgroups: bionet.molbio.ageing Subject: Re: fas gene Message-ID: <2opkt4$hem@scunix2.harvard.edu> From: gwmartin@husc9.harvard.edu (glover martin) Date: 16 Apr 1994 21:21:40 GMT References: <199404152120.OAA26594@net.bio.net> Distribution: bionet Organization: Harvard University, Cambridge, Massachusetts NNTP-Posting-Host: husc9.harvard.edu X-Newsreader: TIN [version 1.2 PL1] Lines: 16 VERPHELD@VTVM1.CC.VT.EDU wrote: : Autoimmune prone MRL/lpr mice have been reported to have deffective Fas genes : which would presumably alter the negative selection process in the thymus. I : would like to know if anybody has any thoughtson why this deffective fas gene w : ould alter T cell development and yet have no effect on other developmental : processes mediatred through apoptosis. : ************************************************************************ : Daniela I. Verthelyi : Virgina Tech. : USA : VERPHERLD@VTVM1 Could there be many ways to start the "program" of apoptosis? If this is the case, could fas be T-cell/T-cell precursor specific? Trei From owner-ageing@net.bio.net Mon Apr 18 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!noc.near.net!news.delphi.com!usenet From: jarice@delphi.com Newsgroups: bionet.molbio.ageing Subject: Re: Aging rates/human vs. dog Date: Tue, 19 Apr 94 01:20:34 -0500 Organization: Delphi (info@delphi.com email, 800-695-4005 voice) Lines: 10 Message-ID: References: <1994Apr14.235840.7253@mr.med.ge.com> NNTP-Posting-Host: delphi.com X-To: David Hinz W-641 548-3221 David Hinz W-641 548-3221 writes: >Why do dogs often have cataracts when they reach the age of, say, 12 years, >but in humans it takes maybe 5 times as long? Are the cells aging at a >different rate, and if so, why? If we knew why, could we do something >about affecting aging rates? One answer may be the much higher levels of antioxidants present in longer-lived animals, including man. Several recent studies also show that antioxidant supplementation may delay or prevent cataract formation. From owner-ageing@net.bio.net Tue Apr 19 23:00:00 1994 Path: biosci!daresbury!not-for-mail From: "Leonid Gavrilov" Newsgroups: bionet.molbio.ageing Subject: Request for assistance Date: 20 Apr 1994 21:16:10 +0100 Organization: A.N.Belozersky Institute, Moscow State University Lines: 50 Sender: daemon@mserv1.dl.ac.uk Distribution: bionet Message-ID: <2p42ia$akk@mserv1.dl.ac.uk> X-Russian: Cyrillic Original-To: AGEING@dl.AC.UK TO: FROM: Dr. Leonid A.Gavrilov, Ph.D. A.N.Belozersky Institute Moscow State University 119899 Moscow, RUSSIA FAX: 7 (095) 939-0338 7 (095) 939-3181 April 20, 1994 Dear Sirs, I am a Russian gerontologist trying to establish international scientific contacts. Since my previous attempts to subscribe to this LISTSERV and to receive the mail address of other subscribers were so far unsuccessful, I would be most grateful for any advice and instruction how to solve this problem. Please send your response to my E-mail address: aeiveos@glas.apc.org Thank you in advance for your kindness. Sincerely yours, Dr.Leonid A.Gavrilov, Ph.D. -- ________________________________________________________________ -- Dr. Leonid A. Gavrilov, Ph.D. Phone: 7 (095) 427 0047 Principal Research Scientist FAX: 7 (095) 939 0338 or A.N.Belozersky Institute 7 (095) 939 3181 Moscow State University E-mail addresses: Moscow 119899 gavrilov@aeiveos.uucp.free.msk.su Russia libro@genebee.msu.su aeiveos@glas.apc.org Please send your answer to my most reliable E-mail address: aeiveos@glas.apc.org if you wish to be sure to reach me. Thank you ! ___________________________________________________________________ From owner-ageing@net.bio.net Wed Apr 20 23:00:00 1994 Path: biosci!daresbury!not-for-mail From: Mail Delivery System Newsgroups: bionet.molbio.ageing Subject: Delivery failure notification Date: 21 Apr 1994 02:32:30 +0100 Lines: 68 Sender: daemon@mserv1.dl.ac.uk Distribution: bionet Message-ID: <2p4l3e$csn@mserv1.dl.ac.uk> Original-To: ----------------------------Original message---------------------------- With reference to your message with the subject: "fas gene" One or more addresses in your message have failed with the following responses from the mail transport system: File creation error delivering to 'jonas'. Should you need assistance, please mail postmaster@alf1.ngate.uni-regensburg.de. -------------------- Returned message follows --------------------- Return-path: Received: from ngate.rz.uni-regensburg.de by alf1.ngate.uni-regensburg.de (Mercury 1.11); Fri, 15 Apr 94 23:55:18 MET-1 Received: from VTVM1.CC.VT.EDU by ngate.rz.uni-regensburg.de; Fri, 15 Apr 94 23:53 GMT Received: from ib.rl.ac.uk by comsun.rz.uni-regensburg.de with SMTP id AA15420 (5.65c/IDA-1.4.4 for ); Fri, 15 Apr 1994 23:53:29 +0200 Received: from letterbox.rl.ac.uk by ib.rl.ac.uk (IBM VM SMTP V2R1) with TCP; Fri, 15 Apr 94 22:56:39 BST Received: from dl.ac.uk by pserv1.dl.ac.uk with SMTP (PP-6.0) id <02854-0@pserv1.dl.ac.uk>; Fri, 15 Apr 1994 22:51:25 +0100 Received: by mserv1.dl.ac.uk id WAA04408 (8.6.6.Beta8/5.3[ref pg@dl.ac.uk] for dl.ac.uk from server-daemon@dl.ac.uk); Fri, 15 Apr 1994 22:49:38 +0100 Received: by mserv1.dl.ac.uk id WAA04394 (8.6.6.Beta8/5.3[ref pg@dl.ac.uk] for dl.ac.uk from server-daemon@dl.ac.uk); Fri, 15 Apr 1994 22:49:07 +0100 Received: by mserv1.dl.ac.uk id WAA04279 (8.6.6.Beta8/5.3[ref pg@dl.ac.uk] for dl.ac.uk from news@dl.ac.uk); Fri, 15 Apr 1994 22:48:26 +0100 Via: uk.ac.dl.pserv1; Fri, 15 Apr 1994 22:49:59 +0100 Resent-From: server-daemon Date: 15 Apr 1994 14:20:27 -0700 Resent-Date: Fri, 15 Apr 94 22:49:32 UT Message-Id: <94415224932.~INN-KREa00158.bionet-news@dl.ac.uk> Resent-Message-Id: <199404152120.OAA26594@net.bio.net> Precedence: list From: VERPHELD@VTVM1.CC.VT.EDU Reply-To: VERPHELD@VTVM1.CC.VT.EDU Original-Sender: "bionet.molbio.ageing mail newsgroup" To: "bionet.molbio.ageing mail newsgroup" Subject: fas gene Comments: List problems/queries to Comments: To mail both the group and netnews send to (ageing@dl.ac.uk) X-Article-Number: bionet.molbio.ageing Msg # 437 X-Listpath: bionet-news X-Mailer: MXT V 12.16.1 Content-Length: 489 Content-Type: text Sender: server-daemon@dl.ac.uk Autoimmune prone MRL/lpr mice have been reported to have deffective Fas genes which would presumably alter the negative selection process in the thymus. I would like to know if anybody has any thoughtson why this deffective fas gene w ould alter T cell development and yet have no effect on other developmental processes mediatred through apoptosis. ************************************************************************ Daniela I. Verthelyi Virgina Tech. USA VERPHERLD@VTVM1 From owner-ageing@net.bio.net Wed Apr 20 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!sunic!pipex!howland.reston.ans.net!gatech!swrinde!ihnp4.ucsd.edu!munnari.oz.au!newsroom.utas.edu.au!sd_chin@postoffice.utas.edu.au From: sd_chin@postoffice.utas.edu.au (Devika Chin) Newsgroups: bionet.molbio.ageing Subject: Autoimmunity in later life? Date: 21 Apr 1994 01:22:42 GMT Organization: University of Tasmania Lines: 7 Distribution: world Message-ID: <2p4kh2$1h1@franklin.cc.utas.edu.au> NNTP-Posting-Host: clinicals100h46.med.utas.edu.au Some investigators have hypothesied that the neuronal death in the aged could be due to autoimmunity that is expressed in later life. Is there any substantial evidence that supports this hypothesis? Does complement activation play a role in causing cell death? From owner-ageing@net.bio.net Fri Apr 22 23:00:00 1994 Path: biosci!daresbury!not-for-mail From: "Leonid Gavrilov" Newsgroups: bionet.molbio.ageing Subject: Looking for scientific contacts Date: 23 Apr 1994 17:40:29 +0100 Organization: A.N.Belozersky Institute, Moscow State University Lines: 322 Sender: daemon@mserv1.dl.ac.uk Distribution: bionet Message-ID: <2pbj1t$2f8@mserv1.dl.ac.uk> X-Russian: Cyrillic Original-To: AGEING@dl.AC.UK April 23, 1994 Dear Sirs, Please allow me to introduce myself. I am a Russian scientist interested in ageing research and a new subscriber to this List. I would like to establish scientific contacts and perhaps scientific collaboration with those who are interested in the problems of longevity and biology of life span. My CV is printed below for more detailed introduction. Sincerely yours, Dr.Leonid A.Gavrilov, Ph.D. Member of the New York Academy of Sciences ********************************************************************* March 26, 1994 C U R R I C U L U M V I T A E Dr. Leonid A. Gavrilov, Ph.D. Phone: 7 (095) 427 0047 Principal Research Scientist FAX: 7 (095) 939 0338 or A.N.Belozersky Institute 7 (095) 939 3181 Moscow State University E-mail addresses: Moscow 119899 libro@genebee.msu.su Russia gavrilov@aeiveos.uucp.free.msk.su aeiveos@glas.apc.org Date of Birth: August 28, 1954 Place of Birth: USSR (Russia), town of Sverdlovsk (now Ekaterinburg) Research interests: Mechanisms of ageing, age-related mortality, longevity. Education: Ph.D. - Moscow State University (1980) in mortality kinetics in human populations and animal models, with emphasis on mathematical models of mortality. M.Sc. - Moscow State University (1976) in mechanisms of aging with emphasis on mathematical models of aging Professional Experience: 1991-Present Principal Research Scientist, A.N.Belozersky Institute, Moscow State University. 1987-1991 Senior Research Scientist, A.N.Belozersky Institute, Moscow State University. 1980-1987 Research Scientist, A.N.Belozersky Institute, Moscow State University. Professional Activities and Other Positions: - Member of the New York Academy of Science. - Chairman of the Subsection of the Biology of Life Span at Moscow Society of Naturalists (1981-1992). - Expert (book reviewer) for journals AGE AND AGEING, AGEING AND SOCIETY, J.GENERAL BIOLOGY and Publishers "MIR". - Expert (peer reviewer) for journals MATHEMATICAL POPULATION STUDIES, J.GENERAL BIOLOGY. - Chairperson of the Biological Session at the II European Congress of Gerontology (Madrid, 1991). Certificate signed by F.Guillen Llera, General Secretary of the Congress. - Expert (book editor) for Institute of Scientific Information (VINITI, Moscow). - Fellow of Moscow Society of Naturalists. - Senior Research Scientist at the Russian Academy of Sciences Institute for System Analysis. - Scientific Secretary of Demographic Section of Moscow House of Scientists (1984-1991). - Fellow of the Moscow House of Scientists. Awards: - International Science Foundation (ISF) 1993 Grant Award ($500). - International Science Foundation (ISF) 1994 Emergency Grant for Russian Scientists of the Top Category ($3,000) - The Certificate from the International Association of Gerontology (1993) signed by the President and the Secretary General of the International Association of Gerontology. - A.N.Belozersky Institute 1991 Award for the best scientific research in the Institute in 1990-1991. - Moscow Society of Naturalists 1989 Award for the best scientific research in natural science in 1986-1988. - Grant Award with invitation to European Population Conference (Paris, October 1991). - Financial Award with invitation of giving a lectures to Trinity College, Cambridge University (December, 1991). - Financial Award with invitation to the Annual Meeting of British Biochemical Society (Southampton, April, 1992). Publications: 65 publications, including 5 books. Selected publications in the most prestigeous scientific peer reviewed journals are: - "Sex and Longevity", Nature, 1994, vol.367, February 10, p.520. - "Fruit Fly Aging and Mortality", Science, 1993, vol.260, No.5114, p.1565. - "When Your Time's Up", British Medical Journal, 1993, vol.306, No.6880, p.796. - "Human Life Span Stopped Increasing: Why?", Gerontology, 1983, vol.29, pp.176-180. - "Life Span is Determined by Resting Metabolic Rate of Parents Only!", Age, 1989, vol.12, p.113. - "Human Species-Specific Life-Span: Its Estimation and Interpretation", Age, 1985, vol.8, p.94. - "A New Trend in Human Mortality Decline: Derectangularization of the Survival Curve", Age, 1985, vol.8, p.93. - "Biology of Aging (Book Review)", Age and Ageing, 1993, vol.22, pp.233-234. - "Evolutionary Biology of Aging (Book Review)", Ageing and Society, 1992, vol.12, No.4, pp.539-541. - "How Many Cell Divisions in 'Old' Cells?", International Journal of Geriatric Psychiatry, 1993, vol.8, No.6, p.528. - "Common Sense and the Limits to Life", International Journal of Geriatric Psychiatry, 1993, vol.8, No.8, p.695. - "Historically Stable Indices of Mortality and their Use for Medical Geography", Geographia Medica, 1984, vol.14, pp.305-306. - "Epidemiologic Approach to the Biology of Human Life Span", Geographia Medica, 1985, vol.15, pp.40-64. The key publication where the main results of the previous studies are summarised is: THE BIOLOGY OF LIFE SPAN: A QUANTITATIVE APPROACH. By Leonid A.Gavrilov and Natalia S.Gavrilova. Harwood Academic Publishers: 1991. Pp. 385, $ 120, ISBN 3-7186-4983-7. Positive reviews of this book were published by the following journals: 1. AGE AND AGEING, 1992, vol.21, No.5, pp.386-387. 2. AGEING AND SOCIETY, 1991, vol.11, No.4, pp.509-510. 3. ARCHIVES OF GERONTOLOGY AND GERIATRICS, 1992, vol.15, No.2, pp.192-194. 4. ARGOMENTI DI GERONTOLOGIA, 1993, vol.5, No.2, p.91. 5. BIOESSAYS, 1993, vol.15, No.5, pp.359-362. 6. BOLLETTINO DELLA SOCIETA ITALIANA DI GERONTOLOGIA E GERIATRIA, 1992, No.7, p.1. 7. BRITISH MEDICAL JOURNAL, 1992, vol.305, No.6850, p.431. 8. EDUCATIONAL GERONTOLOGY, 1993, vol.19, No.1, pp.92-93. 9. EXPERIMENTAL GERONTOLOGY, 1992, vol.27, pp.251-253. 10. FREE RADICAL BIOLOGY & MEDICINE, 1992, vol.12, pp.331-332. 11. GERONTOLOGIST, 1991, vol.31, No.5, p.707. 12. HUMAN BIOLOGY, 1992, vol.64, No.4, pp.630-632. 13. INTERNATIONAL JOURNAL OF GERIATRIC PSYCHIATRY, 1992, vol.7, No.8, p.614. 14. JOURNAL OF CROSS-CULTURAL GERONTOLOGY, 1993, vol.8, No.3, pp.281-290. 15. JOURNAL OF EPIDEMIOLOGY & COMMUNITY HEALTH, 1992, vol.46, No.6, p.630. 16. JOURNAL OF THE INSTITUTE OF ACTUARIES, 1992, vol.119, Part II, pp.379-381. 17. JOURNAL OF ORTHOMOLECULAR MEDICINE, 1993, vol.8, No.1, pp.59-60. 18. NATURE, 1991, vol.352, 29 August, pp.767-768. 19. POPULATION AND DEVELOPMENT REVIEW, 1992, vol.18, No.3, pp.555-558. 20. POPULATION STUDIES, 1992, vol.46, No.2, pp.366-367. 21. QUARTERLY REVIEW OF BIOLOGY, 1993, vol.68, p.92. 22. STATISZTIKAI SZEMLE (STATISTICAL REVIEW, In Hungarian), 1992,vol.70,No.6,pp.546-547. Response of the Leading Scientists to the Scientific Research and the Book by L.A.Gavrilov: "HIGHLY RECOMMENDED FOR STIMULATING FRESH APPROACHES ... " - Professor Roy L.Walford, M.D., UCLA School of Medicine, USA. THE GERONTOLOGIST, 1991, vol.31, No.5, p.707. "CLEARLY, THIS IS A VERY INTERESTING, PROVOCATIVE AND THOUGHT-PROVOKING BOOK" - Dr. Ward Dean, M.D., The Center for Bio-Gerontology, Pencasola, Florida, USA. EXPERIMENTAL GERONTOLOGY, 1992, vol.27, No.2, pp.251-253. "THIS IS AN INTERESTING BOOK, FULL OF TABLES AND DATA AND POLEMICAL IN ITS APPROACH." - Professor William A.Pryor, Director of Biodynamic Institute, Louisiana State University, Baton Rouge, LA,USA. FREE RADICAL BIOLOGY & MEDICINE, 1992, vol.12, pp.331-332. "IT BRINGS TO BEAR A FRESH APPROACH WHOSE IDEAS ARE LOGICALLY PRESENTED AND DEVELOPED". - Dr. Brian Merry, Ph.D., Institute of Human Aging, University of Liverpool, United Kingdom. AGEING AND SOCIETY, 1991, vol.11, No.4, pp.509-510. "GOOD NEW BOOK ON AGEING". - Dr. Thomas B.L.Kirkwood, Ph.D. Head of the Laboratory of Mathematical Biology, National Institute for Medical Research, London, United Kingdom. NATURE, 1991, vol.352, 29 August, pp.767-768. "... SHOULD BE READ BY ANYONE SERIOUSLY INTERESTED IN AGEING. ... YOU WILL FIND THIS A SURPRISINGLY GOOD 'READ', AND A MUST FOR EVERY LIBRARY". - Dr. D.Sebastian Fairweather, Physician, Department of Geriatric Medicine, Oxford, United Kingdom. AGE AND AGEING, 1992, vol.21, No.5, pp.386-387. "THE BOOK FEATURES QUITE A BIT OF DEMOGRAPHIC THEORY AND ANALYSIS. .... IT IS REFRESHING" - Professor Michael R.Rose, University of California, Irvine, USA. ANNALS OF HUMAN BIOLOGY, 1992, vol.19, No.3, pp.320-321. "THE GAVRILOVS HAVE MADE A VALUABLE CONTRIBUTION TO THE STUDY OF LIFE SPAN, AND ANY FUTURE WORK ON THE SUBJECT WILL HAVE TO TAKE ACCOUNT OF THE RICH CONTENTS OF THIS BOOK". - Dr. Vaino Kannisto, Former United Nations Adviser on Demography POPULATION STUDIES, 1992, vol.46, No.2, pp. 366-367. "EIN AKTUELLES BUCH, DAS SICHERLICH DIE INTERNATIONALE DISKUSSION DES PROBLEMS BEFORDERN KANN." - Prof. Dr. Ingeborg Falck, Berlin, Germany. ZEITSCHRIFT FUR GERONTOLOGIE, 1991, Band 25, Heft 1, S.56. "THE BOOK IS WELL WRITTEN, EASY TO READ, AND SHOULD BE OF INTEREST TO A WIDE SPECTRUM OF READERS INCLUDING PHYSICIANS, BIOLOGISTS, BIOCHEMISTS, GERONTOLOGISTS..." - Prof. Robert W.Gracy, University of North Texas, USA. EDUCATIONAL GERONTOLOGY, 1993, vol.19, No.1, pp.92-93. "... THIS BOOK IS A HIGHLY VALUABLE CONTRIBUTION TO THE DISCIPLINE. ...THIS BOOK SHOULD MAKE SCIENTISTS TAKE NOTICE OF RESEARCH ON AGING AND LONGEVITY ... IN THE FORMER SOVIET UNION" - Dr. S.Jay Olshansky, Ph.D., University of Chicago, USA POPULATION AND DEVELOPMENT REVIEW, 1992, vol.18, No.3, pp.555-558. "... MANY WILL FIND AN EXCURSION INTO IT STIMULATING". - Dr. Emily Grundy, Lecturer in Gerontology, Age Concern Institute of Gerontology, King's College, London, UK. BRITISH MEDICAL JOURNAL, 1992, vol.305, No.6850, p.431. "GAVRILOV MAKES THE BEST ATTEMPT I KNOW OF TO DISTINGUISH HOW LONG PEOPLE COULD LIVE FROM HOW LONG THEY ACTUALLY LIVE - ONE OF THE MORE DIFFICULT TASKS FOR BOTH BIOLOGY AND STATISTICS... GAVRILOV'S SCHOLARSHIP IS IMPRESSIVE". - Professor Nathan Keyfitz, International Institute for Applied Systems Analysis, Laxenburg, Austria. MATHEMATICAL POPULATION STUDIES, 1991, vol.3, No.2, p.161. "LEONID GAVRILOV IS A TOP-FLIGHT STATISTICAL POPULATION BIOLOGIST... THE AREA IS IMPORTANT WELL BEYOND THE CONFINES OF BIOLOGICAL GERONTOLOGY AND EXTENDS INTO POLITICS AND SOCIAL PLANNING. GAVRILOV IS VERY GOOD ON JUST THIS SORT OF THING. HE HAS A FRESH APPROACH, NEW IDEAS, A GREAT DEAL OF DATA NOT READILY AVAILABLE ELSEWHERE." - Roy. L. Walford, Professor of Pathology, UCLA School of Medicine, USA. MATHEMATICAL POPULATION STUDIES, 1991, vol.3, No.2, p.161. "THIS BOOK IS DIRECTED TOWARD RESEARCHERS INTERESTED IN STATISTICAL PATTERNS AND MATHEMATICAL MODELS OF HUMAN LIFE SPAN. FOR STUDENTS WITHIN THIS DEFINED AREA OF RESEARCH, THIS TEXT OFFERS A GOOD HISTORICAL PERSPECTIVE, INSIGHT INTO THE RUSSIAN LITERATURE, AND INSIGHT INTO THE QUANTITATIVE ANALYSIS OF LIFE SPAN" - Dr. Deborah A.Roach, Department of Zoology, Duke University, Durham, USA. ECOLOGY, 1992, vol.73, No.1, pp.379-381. "THIS BOOK IS IN ESSENCE A BIOSTATISTICAL ANALYSIS OF THE PROBLEMS OF LIFESPAN, HUMAN AND ANIMAL. ... THE LITERATURE IN MANY LANGUAGES HAD CLEARLY BEEN WELL COVERED, AND MUCH HAS BEEN MADE OF NATIONAL AND OTHER MORTALITY STATISTICS IN THIS CENTURY. ... A USEFUL SOURCE BOOK." - Dr. F.I.Caird, D.M., F.R.C.P., Professor of Geriatric Medicine, Southern General Hospital, Glasgow, UK. J. OF CLINICAL AND EXPERIMENTAL GERONTOLOGY, 1992, vol.14, No. 3 & 4, p.309. "... THE READER WILL BENEFIT ENORMOUSLY FROM THE BROADENING OF PERSPECTIVE AS A RESULT OF EXPOSURE TO THE GAVRILOVS' THEORIES ON THE BIOLOGY OF LIFE SPAN" - Dr. M. Michael Akiyama, University of Michigan, Dearborn, USA. HUMAN BIOLOGY, 1992, vol.64, No.4, pp.630-632. "THIS IS A PROVOCATIVE AND REFRESHING BOOK" - Dr.Alan R.Hipkiss, Age Concern Institute of Gerontology, King's College London, UK. INTERNATIONAL JOURNAL OF GERIATRIC PSYCHIATRY, 1992, vol.7,No.8,p.614. "THIS BOOK SHOULD BE READ BY ALL MEMBERS OF THE PROFESSION INTERESTED IN MORTALITY - WHICH SHOULD BE ALL MEMBERS OF THE PROFESSION" - Dr. H.A.R.Barnett, Institute of Actuaries, UK. J.OF THE INSTITUTE OF ACTUARIES, 1992, vol.119, Part II, pp.379-381. "TO SUM UP, THE BOOK IS SUBSTANTIAL, INTERESTING AND BRINGS FRESH AIR. WE CAN CONFIDENTLY COMMEND ITS STUDY TO ALL SCIENTISTS ... " - Dr. Janos Izsak, Department of Zoology, Berzsenyi Teachers' College, Szombathely, Hungary. ARCHIVES OF GERONTOLOGY AND GERIATRICS, 1992, vol.15, No.2,pp.192-194. "A GENERAL THEORY OF LIFESPAN IS THUS CREATED." - - Dr. Norman Vetter, University Hospital of Wales, Cardiff, UK. J.OF EPIDEMIOLOGY & COMMUNITY HEALTH, 1992, vol.46, No.6, p.630. "I FOUND IT TO BE HIGHLY REWARDING READING." - Dr. Edward J.Masoro, University of Texas Health Science Center. QUARTERLY REVIEW OF BIOLOGY, 1993, vol.68, p.92. "THIS ... IS AN EXCELLENT BOOK. ... NOBODY INTERESTED IN AGING SHOULD FAIL TO READ". - Dr. C.S.Downes, Department of Zoology, University of Cambridge, UK. BIOESSAYS, 1993, vol.15, No.5, pp.359-362. "...PROVOCATIVE AND ENTERTAINING READING AND CAN BE RECOMMENDED TO ANYONE WITH AN INTEREST IN BIOLOGICAL AGING." - Dr. Douglas E.Crews, Department of Anthropology, Ohio State University, USA JOURNAL OF CROSS-CULTURAL GERONTOLOGY, 1993, vol.8, No.3, pp.281-290. "IT DESERVES TO BE READ WIDELY. SCIENTISTS AND PHYSICIANS WHO ARE INTERESTED IN THE AGING OF POPULATIONS OR OF INDIVIDUALS WILL BE MUCH MORE EFFECTIVE IN THEIR WORK IF THEY BECOME FAMILIAR WITH THE SUBJECT MATTER OF THIS BOOK." - JOURNAL OF ORTHOMOLECULAR MEDICINE, 1993, vol.8, No.1, pp.59-60. *********************************THE END************************************ From owner-ageing@net.bio.net Fri Apr 22 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!gatech!newsxfer.itd.umich.edu!jobone!lynx.unm.edu!news.cs.indiana.edu!babbage.ece.uc.edu!mary.iia.org!rtp.vnet.net!news.sprintlink.net!news.world.net!news.teleport.com!news.teleport.com!not-for-mail From: jor@teleport.com (Jo Robinson) Newsgroups: bionet.molbio.ageing Subject: melatonin Date: 23 Apr 1994 15:13:08 -0700 Organization: Teleport - Portland's Public Access (503) 220-1016 Lines: 13 Message-ID: <2pc6hk$hrp@linda.teleport.com> NNTP-Posting-Host: linda.teleport.com X-Newsreader: TIN [version 1.2 PL2] I am a freelance journalist researching a book for the educated public on melatonin, the pineal hormone. I am interested in its anti-aging, oncostatic, immunoenhancing, antioxidant properties. I would very much like to talk to researchers involved -- or interested -- in this fast-growing field. I'm particularly interested in finding out if anyone is doing primate or human longevity trials. But any input is appreciated. -- +-----------------------------------+-----------------------------------------+ | Jo Robinson | jor@teleport.com | | (503)284-4676 | 2826 NE 18th Portland, OR 97212 | +-----------------------------------+------------------------------------y-----+ From owner-ageing@net.bio.net Sat Apr 23 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!pipex!uknet!daresbury!not-for-mail From: "Leonid Gavrilov" Newsgroups: bionet.molbio.ageing Subject: Urgent grant for ageing research Date: 24 Apr 1994 09:14:40 +0100 Organization: A.N.Belozersky Institute, Moscow State University Lines: 31 Sender: daemon@mserv1.dl.ac.uk Distribution: bionet Message-ID: <2pd9pg$1j2@mserv1.dl.ac.uk> X-Russian: Cyrillic Original-To: AGEING@dl.AC.UK April 24, 1994 Dear Sirs, The purpose of this message is to invite you to join a research team for receiving EC grant called COPERNICUS. In order to receive this grant a team of 5 researchers from 5 different countries should be created: 2 researchers from EC countries, 2 researchers from Eastern European countries and 1 researcher from the countries of the former Soviet Union (myself). Additional researchers from other eligible countries are very much wellcome. Of special importance is participant from Belgium since the COPERNICUS Headquarters is located there and it is extremely important to have an opportunity for personal contact with COPERNICUS administration. The suggested topics of the grants are: 1. Parental age an offspring longevity. 2. Biomedical basis for sex differences in life span. 3. Any other topics could be negotiated. The deadline for application is May 2, 1994. So, we are to be very rapid ! Sincerely yours, Dr.Leonid A.Gavrilov, Ph.D. Member of the New York Academy of Sciences P.S.: My CV is available upon the request or could be extracted from archives of this LIST (message 447 of April 23). From owner-ageing@net.bio.net Sat Apr 23 23:00:00 1994 Path: biosci!DRUID.HSC.COLORADO.EDU!mandy From: mandy@DRUID.HSC.COLORADO.EDU (Mandy Caird PhD) Newsgroups: bionet.molbio.ageing Subject: CANCER, AGING AND TH Date: 24 Apr 1994 12:27:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 63 Sender: daemon@net.bio.net Distribution: bionet Message-ID: Reply-To: Mandy Caird PhD NNTP-Posting-Host: net.bio.net Dear all on the ageing list! I recently read an article in U.S. News and World Report on immortal cancer cells. The article went on to discuss how cancer cells are immortal because the produce the Telomerase enzyme which rejuvenates the Telomeres at the ends of the DNA strands in the cells which prevents the aging or senecesing of the cells. The article further discussed how this may allow researchers to target cancer cells since most human cells no longer produce Telomerase and are mortal. It may eventually may lead to a more specific way of fighting cancer, as opposed to the blunt approach of chemotherapy. A side issue dealt with in the article was that almost all cells in an adult human no longer produce Telomerase and are mortal, with the exception of certain cells in bone marrow and the male testicles. Mortal cells can only reproduce 50 to 100 times before degrading quite rapidly, whereas the cancer cells can reproduce acurately forever. This raised several questions in my mind. I realize that there is probably no current yes or no answers to these questions, but your insight and informed commentary on my questions would be greatly appreciated. My questions are as follows: 1. Is there a direct correlation between human aging (growing old) and the shorten of Telomeres thru cellular reproduction in normal human mortal cells? 2. If the enzyme telomerase were introduced into a normal mortal human cells could it extend the longevity and quality of a human life by repairing chromosomal instability and chromosome damage? Perhaps, eliminate death from old age? 2. a) Would this enzyme treatment only be temporary or permanent, and if it is temporary would regular and periodic introduction of this enzyme into the cell perform the same function as if the Telomerase enzyme were being constantly produced within the cell itself? 3. Would the introduction of the enzyme Telomerase into a mortal human cell stabilize the Telomeres at the ends of the DNA at their current length or at a shorter or longer length? 4. Is it feasable to reactivate the dormant chromosome responsible for the production of telomerase. 5. In addition to the potential of Telomerase being able to immortalize human mortal cells allowing them to replicate their DNA sequences accurately forever, would there be any potential side effects that would diminish the possible positive benefits from having this enzyme present in human cells? Thanks!!! Sincerely, Carl W. Weaver From owner-ageing@net.bio.net Sat Apr 23 23:00:00 1994 Path: biosci!DRUID.HSC.COLORADO.EDU!mandy From: mandy@DRUID.HSC.COLORADO.EDU (Mandy Caird PhD) Newsgroups: bionet.molbio.ageing Subject: Re: CANCER, AGING AND TH Date: 24 Apr 1994 14:16:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: bionet Message-ID: NNTP-Posting-Host: net.bio.net Please reply to MR CARL W WEAVER From owner-ageing@net.bio.net Sun Apr 24 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!pipex!doc.ic.ac.uk!susx.ac.uk!bafa1 From: bafa1@central.susx.ac.uk (Sydney Shall) Newsgroups: bionet.molbio.ageing Subject: Telomerase Date: 25 Apr 1994 09:09:28 GMT Organization: University of Sussex Lines: 26 Message-ID: <2pg1c8$kbu@infa.central.susx.ac.uk> NNTP-Posting-Host: solx1.central.susx.ac.uk X-Newsreader: TIN [version 1.2 PL2] The enzyme telomerase has been shown to be present in immortal cell lines. However, it has never been purified and therefore it has never been cloned. The average length of telomeres in dividing normal, untransformed cells has been shown to shorten as the cell culture ages. That is, there is a correlation between the length of the average telomere size and the age of the culture. The predictions of what the re-introduction of a telomerase enzyme might do in ageing cells requires the cloning of the gene for this enzyme. This is proving a very difficult task. In presence it should be possible to reactivate the expression of this gene in normal cells, and to repress its expression in cancer cells, but this also awaits the cloning of the gene. This area is therefore both very active and very excitibg, and there is lots of room for more people in this field. -- ************************************************************************** ************************************************************************** From owner-ageing@net.bio.net Sun Apr 24 23:00:00 1994 Path: biosci!agate!dog.ee.lbl.gov!ihnp4.ucsd.edu!swrinde!cs.utexas.edu!howland.reston.ans.net!pipex!uknet!daresbury!not-for-mail From: "Leonid Gavrilov" Newsgroups: bionet.molbio.ageing Subject: Hayflick limit wrong ? Date: 25 Apr 1994 17:32:33 +0100 Organization: A.N.Belozersky Institute, Moscow State University Lines: 50 Sender: daemon@mserv1.dl.ac.uk Distribution: bionet Message-ID: <2pgrb1$p2o@mserv1.dl.ac.uk> X-Russian: Cyrillic Original-To: AGEING@dl.AC.UK Dear AGEING LIST subscribers, I would like to know the current situation for the discovery of the authors mentioned below, that simple change of composition of the cell culture medium could eliminate cellular senescence: Loo, D.T., Fuquay, J.I., Rawson, C.L. and Barnes, D.W. (1987). Extended culture of mouse embryo cells without senescence: inhibition by serum. Science 236, 200-202. These authors demonstrated that normal diploid mouse embryo cells, which under standard conditions manifest a growth crisis after 7-10 population doublings, may be successfully cultivated without any sign of an approaching growth crisis for at least 200 population doublings. All that is necessary is to change the composition of the culture medium (excluding blood serum and adding a number of ingredients, including the epidermal growth factor). In this case the cells, which are apparently capable of unlimited multiplication, remain diploid and nontumorigenic. If that is true, then the claim of Dr.Hayflick that all diploid cells have intrinsic limit to cell division, is definitely wrong. Does anybody know whether the results of these authors were reproduced or disproved ? Does anybody knows the present addresses (E-mail in particular) of these authors ? Thanks, Dr.Leonid A.Gavrilov, Ph.D. -- ________________________________________________________________ -- Dr. Leonid A. Gavrilov, Ph.D. Phone: 7 (095) 427 0047 Principal Research Scientist FAX: 7 (095) 939 0338 or A.N.Belozersky Institute 7 (095) 939 3181 Moscow State University E-mail addresses: Moscow 119899 gavrilov@aeiveos.uucp.free.msk.su Russia libro@genebee.msu.su aeiveos@glas.apc.org Please send your answer to my most reliable E-mail address: aeiveos@glas.apc.org if you wish to be sure to reach me. Thank you ! ___________________________________________________________________ From owner-ageing@net.bio.net Sun Apr 24 23:00:00 1994 Path: biosci!aeiveos.uucp.free.msk.su!gavrilov From: gavrilov@aeiveos.uucp.free.msk.su ("Leonid Gavrilov") Newsgroups: bionet.molbio.ageing Subject: Grant for Longevity Research Date: 25 Apr 1994 10:38:19 -0700 Organization: A.N.Belozersky Institute, Moscow State University Lines: 335 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <2dba3297.aeiveos@aeiveos.uucp.free.msk.su> Reply-To: "Leonid Gavrilov" NNTP-Posting-Host: net.bio.net April 24, 1994 Dear Sirs, The purpose of this message is to invite you to join a research team for receiving EC grant called COPERNICUS. In order to receive this grant a team of 5 researchers from 5 different countries should be created: 2 researchers from EC countries, 2 researchers from Eastern European countries and 1 researcher from the countries of the former Soviet Union (myself). Additional researchers from other eligible countries are very much wellcome. Of special importance is participant from Belgium since the COPERNICUS Headquarters is located there and it is extremely important to have an opportunity for personal contact with COPERNICUS administration. The suggested topics of the grants are: 1. Parental age an offspring longevity. 2. Biomedical basis for sex differences in life span. 3. Any other topics could be negotiated. The deadline for application is May 2, 1994. So, we are to be very rapid ! Sincerely yours, Dr.Leonid A.Gavrilov, Ph.D. Member of the New York Academy of Sciences P.S.: My CV is printed below for introduction. ************************************************************************** March 26, 1994 C U R R I C U L U M V I T A E Dr. Leonid A. Gavrilov, Ph.D. Phone: 7 (095) 427 0047 Principal Research Scientist FAX: 7 (095) 939 0338 or A.N.Belozersky Institute 7 (095) 939 3181 Moscow State University E-mail addresses: Moscow 119899 libro@genebee.msu.su Russia gavrilov@aeiveos.uucp.free.msk.su aeiveos@glas.apc.org Date of Birth: August 28, 1954 Place of Birth: USSR (Russia), town of Sverdlovsk (now Ekaterinburg) Research interests: Mechanisms of ageing, age-related mortality, longevity. Education: Ph.D. - Moscow State University (1980) in mortality kinetics in human populations and animal models, with emphasis on mathematical models of mortality. M.Sc. - Moscow State University (1976) in mechanisms of aging with emphasis on mathematical models of aging Professional Experience: 1991-Present Principal Research Scientist, A.N.Belozersky Institute, Moscow State University. 1987-1991 Senior Research Scientist, A.N.Belozersky Institute, Moscow State University. 1980-1987 Research Scientist, A.N.Belozersky Institute, Moscow State University. Professional Activities and Other Positions: - Member of the New York Academy of Science. - Chairman of the Subsection of the Biology of Life Span at Moscow Society of Naturalists (1981-1992). - Expert (book reviewer) for journals AGE AND AGEING, AGEING AND SOCIETY, J.GENERAL BIOLOGY and Publishers "MIR". - Expert (peer reviewer) for journals MATHEMATICAL POPULATION STUDIES, J.GENERAL BIOLOGY. - Chairperson of the Biological Session at the II European Congress of Gerontology (Madrid, 1991). Certificate signed by F.Guillen Llera, General Secretary of the Congress. - Expert (book editor) for Institute of Scientific Information (VINITI, Moscow). - Fellow of Moscow Society of Naturalists. - Senior Research Scientist at the Russian Academy of Sciences Institute for System Analysis. - Scientific Secretary of Demographic Section of Moscow House of Scientists (1984-1991). - Fellow of the Moscow House of Scientists. Awards: - International Science Foundation (ISF) 1993 Grant Award ($500). - International Science Foundation (ISF) 1994 Emergency Grant for Russian Scientists of the Top Category ($3,000) - The Certificate from the International Association of Gerontology (1993) signed by the President and the Secretary General of the International Association of Gerontology. - A.N.Belozersky Institute 1991 Award for the best scientific research in the Institute in 1990-1991. - Moscow Society of Naturalists 1989 Award for the best scientific research in natural science in 1986-1988. - Grant Award with invitation to European Population Conference (Paris, October 1991). - Financial Award with invitation of giving a lectures to Trinity College, Cambridge University (December, 1991). - Financial Award with invitation to the Annual Meeting of British Biochemical Society (Southampton, April, 1992). Publications: 65 publications, including 5 books. Selected publications in the most prestigeous scientific peer reviewed journals are: - "Sex and Longevity", Nature, 1994, vol.367, February 10, p.520. - "Fruit Fly Aging and Mortality", Science, 1993, vol.260, No.5114, p.1565. - "When Your Time's Up", British Medical Journal, 1993, vol.306, No.6880, p.796. - "Human Life Span Stopped Increasing: Why?", Gerontology, 1983, vol.29, pp.176-180. - "Life Span is Determined by Resting Metabolic Rate of Parents Only!", Age, 1989, vol.12, p.113. - "Human Species-Specific Life-Span: Its Estimation and Interpretation", Age, 1985, vol.8, p.94. - "A New Trend in Human Mortality Decline: Derectangularization of the Survival Curve", Age, 1985, vol.8, p.93. - "Biology of Aging (Book Review)", Age and Ageing, 1993, vol.22, pp.233-234. - "Evolutionary Biology of Aging (Book Review)", Ageing and Society, 1992, vol.12, No.4, pp.539-541. - "How Many Cell Divisions in 'Old' Cells?", International Journal of Geriatric Psychiatry, 1993, vol.8, No.6, p.528. - "Common Sense and the Limits to Life", International Journal of Geriatric Psychiatry, 1993, vol.8, No.8, p.695. - "Historically Stable Indices of Mortality and their Use for Medical Geography", Geographia Medica, 1984, vol.14, pp.305-306. - "Epidemiologic Approach to the Biology of Human Life Span", Geographia Medica, 1985, vol.15, pp.40-64. The key publication where the main results of the previous studies are summarised is: THE BIOLOGY OF LIFE SPAN: A QUANTITATIVE APPROACH. By Leonid A.Gavrilov and Natalia S.Gavrilova. Harwood Academic Publishers: 1991. Pp. 385, $ 120, ISBN 3-7186-4983-7. Positive reviews of this book were published by the following journals: 1. AGE AND AGEING, 1992, vol.21, No.5, pp.386-387. 2. AGEING AND SOCIETY, 1991, vol.11, No.4, pp.509-510. 3. ARCHIVES OF GERONTOLOGY AND GERIATRICS, 1992, vol.15, No.2, pp.192-194. 4. ARGOMENTI DI GERONTOLOGIA, 1993, vol.5, No.2, p.91. 5. BIOESSAYS, 1993, vol.15, No.5, pp.359-362. 6. BOLLETTINO DELLA SOCIETA ITALIANA DI GERONTOLOGIA E GERIATRIA, 1992, No.7, p.1. 7. BRITISH MEDICAL JOURNAL, 1992, vol.305, No.6850, p.431. 8. EDUCATIONAL GERONTOLOGY, 1993, vol.19, No.1, pp.92-93. 9. EXPERIMENTAL GERONTOLOGY, 1992, vol.27, pp.251-253. 10. FREE RADICAL BIOLOGY & MEDICINE, 1992, vol.12, pp.331-332. 11. GERONTOLOGIST, 1991, vol.31, No.5, p.707. 12. HUMAN BIOLOGY, 1992, vol.64, No.4, pp.630-632. 13. INTERNATIONAL JOURNAL OF GERIATRIC PSYCHIATRY, 1992, vol.7, No.8, p.614. 14. JOURNAL OF CROSS-CULTURAL GERONTOLOGY, 1993, vol.8, No.3, pp.281-290. 15. JOURNAL OF EPIDEMIOLOGY & COMMUNITY HEALTH, 1992, vol.46, No.6, p.630. 16. JOURNAL OF THE INSTITUTE OF ACTUARIES, 1992, vol.119, Part II, pp.379-381. 17. JOURNAL OF ORTHOMOLECULAR MEDICINE, 1993, vol.8, No.1, pp.59-60. 18. NATURE, 1991, vol.352, 29 August, pp.767-768. 19. POPULATION AND DEVELOPMENT REVIEW, 1992, vol.18, No.3, pp.555-558. 20. POPULATION STUDIES, 1992, vol.46, No.2, pp.366-367. 21. QUARTERLY REVIEW OF BIOLOGY, 1993, vol.68, p.92. 22. STATISZTIKAI SZEMLE (STATISTICAL REVIEW, In Hungarian), 1992,vol.70,No.6,pp.546-547. Response of the Leading Scientists to the Scientific Research and the Book by L.A.Gavrilov: "HIGHLY RECOMMENDED FOR STIMULATING FRESH APPROACHES ... " - Professor Roy L.Walford, M.D., UCLA School of Medicine, USA. THE GERONTOLOGIST, 1991, vol.31, No.5, p.707. "CLEARLY, THIS IS A VERY INTERESTING, PROVOCATIVE AND THOUGHT-PROVOKING BOOK" - Dr. Ward Dean, M.D., The Center for Bio-Gerontology, Pencasola, Florida, USA. EXPERIMENTAL GERONTOLOGY, 1992, vol.27, No.2, pp.251-253. "THIS IS AN INTERESTING BOOK, FULL OF TABLES AND DATA AND POLEMICAL IN ITS APPROACH." - Professor William A.Pryor, Director of Biodynamic Institute, Louisiana State University, Baton Rouge, LA,USA. FREE RADICAL BIOLOGY & MEDICINE, 1992, vol.12, pp.331-332. "IT BRINGS TO BEAR A FRESH APPROACH WHOSE IDEAS ARE LOGICALLY PRESENTED AND DEVELOPED". - Dr. Brian Merry, Ph.D., Institute of Human Aging, University of Liverpool, United Kingdom. AGEING AND SOCIETY, 1991, vol.11, No.4, pp.509-510. "GOOD NEW BOOK ON AGEING". - Dr. Thomas B.L.Kirkwood, Ph.D. Head of the Laboratory of Mathematical Biology, National Institute for Medical Research, London, United Kingdom. NATURE, 1991, vol.352, 29 August, pp.767-768. "... SHOULD BE READ BY ANYONE SERIOUSLY INTERESTED IN AGEING. ... YOU WILL FIND THIS A SURPRISINGLY GOOD 'READ', AND A MUST FOR EVERY LIBRARY". - Dr. D.Sebastian Fairweather, Physician, Department of Geriatric Medicine, Oxford, United Kingdom. AGE AND AGEING, 1992, vol.21, No.5, pp.386-387. "THE BOOK FEATURES QUITE A BIT OF DEMOGRAPHIC THEORY AND ANALYSIS. ... IT IS REFRESHING" - Professor Michael R.Rose, University of California, Irvine, USA. ANNALS OF HUMAN BIOLOGY, 1992, vol.19, No.3, pp.320-321. "THE GAVRILOVS HAVE MADE A VALUABLE CONTRIBUTION TO THE STUDY OF LIFE SPAN, AND ANY FUTURE WORK ON THE SUBJECT WILL HAVE TO TAKE ACCOUNT OF THE RICH CONTENTS OF THIS BOOK". - Dr. Vaino Kannisto, Former United Nations Adviser on Demography POPULATION STUDIES, 1992, vol.46, No.2, pp. 366-367. "EIN AKTUELLES BUCH, DAS SICHERLICH DIE INTERNATIONALE DISKUSSION DES PROBLEMS BEFORDERN KANN." - Prof. Dr. Ingeborg Falck, Berlin, Germany. ZEITSCHRIFT FUR GERONTOLOGIE, 1991, Band 25, Heft 1, S.56. "THE BOOK IS WELL WRITTEN, EASY TO READ, AND SHOULD BE OF INTEREST TO A WIDE SPECTRUM OF READERS INCLUDING PHYSICIANS, BIOLOGISTS, BIOCHEMISTS, GERONTOLOGISTS..." - Prof. Robert W.Gracy, University of North Texas, USA. EDUCATIONAL GERONTOLOGY, 1993, vol.19, No.1, pp.92-93. "... THIS BOOK IS A HIGHLY VALUABLE CONTRIBUTION TO THE DISCIPLINE. ...THIS BOOK SHOULD MAKE SCIENTISTS TAKE NOTICE OF RESEARCH ON AGING AND LONGEVITY ... IN THE FORMER SOVIET UNION" - Dr. S.Jay Olshansky, Ph.D., University of Chicago, USA POPULATION AND DEVELOPMENT REVIEW, 1992, vol.18, No.3, pp.555-558. "... MANY WILL FIND AN EXCURSION INTO IT STIMULATING". - Dr. Emily Grundy, Lecturer in Gerontology, Age Concern Institute of Gerontology, King's College, London, UK. BRITISH MEDICAL JOURNAL, 1992, vol.305, No.6850, p.431. "GAVRILOV MAKES THE BEST ATTEMPT I KNOW OF TO DISTINGUISH HOW LONG PEOPLE COULD LIVE FROM HOW LONG THEY ACTUALLY LIVE - ONE OF THE MORE DIFFICULT TASKS FOR BOTH BIOLOGY AND STATISTICS... GAVRILOV'S SCHOLARSHIP IS IMPRESSIVE". - Professor Nathan Keyfitz, International Institute for Applied Systems Analysis, Laxenburg, Austria. MATHEMATICAL POPULATION STUDIES, 1991, vol.3, No.2, p.161. "LEONID GAVRILOV IS A TOP-FLIGHT STATISTICAL POPULATION BIOLOGIST... THE AREA IS IMPORTANT WELL BEYOND THE CONFINES OF BIOLOGICAL GERONTOLOGY AND EXTENDS INTO POLITICS AND SOCIAL PLANNING. GAVRILOV IS VERY GOOD ON JUST THIS SORT OF THING. HE HAS A FRESH APPROACH, NEW IDEAS, A GREAT DEAL OF DATA NOT READILY AVAILABLE ELSEWHERE." - Roy. L. Walford, Professor of Pathology, UCLA School of Medicine, USA. MATHEMATICAL POPULATION STUDIES, 1991, vol.3, No.2, p.161. "THIS BOOK IS DIRECTED TOWARD RESEARCHERS INTERESTED IN STATISTICAL PATTERNS AND MATHEMATICAL MODELS OF HUMAN LIFE SPAN. FOR STUDENTS WITHIN THIS DEFINED AREA OF RESEARCH, THIS TEXT OFFERS A GOOD HISTORICAL PERSPECTIVE, INSIGHT INTO THE RUSSIAN LITERATURE, AND INSIGHT INTO THE QUANTITATIVE ANALYSIS OF LIFE SPAN" - Dr. Deborah A.Roach, Department of Zoology, Duke University, Durham, USA. ECOLOGY, 1992, vol.73, No.1, pp.379-381. "THIS BOOK IS IN ESSENCE A BIOSTATISTICAL ANALYSIS OF THE PROBLEMS OF LIFESPAN, HUMAN AND ANIMAL. ... THE LITERATURE IN MANY LANGUAGES HAD CLEARLY BEEN WELL COVERED, AND MUCH HAS BEEN MADE OF NATIONAL AND OTHER MORTALITY STATISTICS IN THIS CENTURY. ... A USEFUL SOURCE BOOK." - Dr. F.I.Caird, D.M., F.R.C.P., Professor of Geriatric Medicine, Southern General Hospital, Glasgow, UK. J. OF CLINICAL AND EXPERIMENTAL GERONTOLOGY, 1992, vol.14, No. 3 & 4, p.309. "... THE READER WILL BENEFIT ENORMOUSLY FROM THE BROADENING OF PERSPECTIVE AS A RESULT OF EXPOSURE TO THE GAVRILOVS' THEORIES ON THE BIOLOGY OF LIFE SPAN" - Dr. M. Michael Akiyama, University of Michigan, Dearborn, USA. HUMAN BIOLOGY, 1992, vol.64, No.4, pp.630-632. "THIS IS A PROVOCATIVE AND REFRESHING BOOK" - Dr.Alan R.Hipkiss, Age Concern Institute of Gerontology, King's College London, UK. INTERNATIONAL JOURNAL OF GERIATRIC PSYCHIATRY, 1992, vol.7,No.8,p.614. "THIS BOOK SHOULD BE READ BY ALL MEMBERS OF THE PROFESSION INTERESTED IN MORTALITY - WHICH SHOULD BE ALL MEMBERS OF THE PROFESSION" - Dr. H.A.R.Barnett, Institute of Actuaries, UK. J.OF THE INSTITUTE OF ACTUARIES, 1992, vol.119, Part II, pp.379-381. "TO SUM UP, THE BOOK IS SUBSTANTIAL, INTERESTING AND BRINGS FRESH AIR. WE CAN CONFIDENTLY COMMEND ITS STUDY TO ALL SCIENTISTS ... " - Dr. Janos Izsak, Department of Zoology, Berzsenyi Teachers' College, Szombathely, Hungary. ARCHIVES OF GERONTOLOGY AND GERIATRICS, 1992, vol.15, No.2,pp.192-194. "A GENERAL THEORY OF LIFESPAN IS THUS CREATED." - - Dr. Norman Vetter, University Hospital of Wales, Cardiff, UK. J.OF EPIDEMIOLOGY & COMMUNITY HEALTH, 1992, vol.46, No.6, p.630. "I FOUND IT TO BE HIGHLY REWARDING READING." - Dr. Edward J.Masoro, University of Texas Health Science Center. QUARTERLY REVIEW OF BIOLOGY, 1993, vol.68, p.92. "THIS ... IS AN EXCELLENT BOOK. ... NOBODY INTERESTED IN AGING SHOULD FAIL TO READ". - Dr. C.S.Downes, Department of Zoology, University of Cambridge, UK. BIOESSAYS, 1993, vol.15, No.5, pp.359-362. "...PROVOCATIVE AND ENTERTAINING READING AND CAN BE RECOMMENDED TO ANYONE WITH AN INTEREST IN BIOLOGICAL AGING." - Dr. Douglas E.Crews, Department of Anthropology, Ohio State University, USA JOURNAL OF CROSS-CULTURAL GERONTOLOGY, 1993, vol.8, No.3, pp.281-290. "IT DESERVES TO BE READ WIDELY. SCIENTISTS AND PHYSICIANS WHO ARE INTERESTED IN THE AGING OF POPULATIONS OR OF INDIVIDUALS WILL BE MUCH MORE EFFECTIVE IN THEIR WORK IF THEY BECOME FAMILIAR WITH THE SUBJECT MATTER OF THIS BOOK." - JOURNAL OF ORTHOMOLECULAR MEDICINE, 1993, vol.8, No.1, pp.59-60. *********************************THE END************************************ From owner-ageing@net.bio.net Sun Apr 24 23:00:00 1994 Path: biosci!daresbury!trane.uninett.no!eunet.no!nuug!EU.net!howland.reston.ans.net!europa.eng.gtefsd.com!library.ucla.edu!csulb.edu!paris.ics.uci.edu!news.claremont.edu!nntp-server.caltech.edu!mac9.bio.caltech.edu!user From: grovesa@starbase1.caltech.edu (Andrew K. Groves) Newsgroups: bionet.molbio.ageing Subject: Re: Hayflick limit wrong ? Followup-To: bionet.molbio.ageing Date: 25 Apr 1994 21:22:41 GMT Organization: California Institute of Technology Lines: 66 Distribution: bionet Message-ID: References: <2pgrb1$p2o@mserv1.dl.ac.uk> NNTP-Posting-Host: mac9.bio.caltech.edu In article <2pgrb1$p2o@mserv1.dl.ac.uk>, "Leonid Gavrilov" wrote: > > > Dear AGEING LIST subscribers, > > I would like to know the current situation for the discovery of the > authors mentioned below, that simple change of composition of the cell > culture medium could eliminate cellular senescence: > > Loo, D.T., Fuquay, J.I., Rawson, C.L. and Barnes, D.W. (1987). > Extended culture of mouse embryo cells without senescence: inhibition by > serum. Science 236, 200-202. > > These authors demonstrated that normal diploid mouse embryo cells, which > under standard conditions manifest a growth crisis after 7-10 population > doublings, may be successfully cultivated without any sign of an > approaching growth crisis for at least 200 population doublings. All that is > necessary is to change the composition of the culture medium (excluding > blood serum and adding a number of ingredients, including the epidermal > growth factor). In this case the cells, which are apparently capable of > unlimited multiplication, remain diploid and nontumorigenic. > > If that is true, then the claim of Dr.Hayflick that all diploid cells > have intrinsic limit to cell division, is definitely wrong. > > Does anybody know whether the results of these authors were reproduced > or disproved ? > Leonid, That paper hasn't been 'disproved' as such, but the findings have been put in a different perspective. In 1990 David Barnes' group published another paper showing that the cells referred to in their Science paper could be induced to express GFAP (glial fibrillary acidic protein) in the presence of serum or TGF-beta. The reference is Sakai et al., PNAS 87 8378-8392. In the original Science paper, I believe the authors simply took mouse embryo carcasses (including the head and spinal cord) and grew them in their defined medium. In the light of the induction of GFAP, it would appear that what they were growing were not fibroblasts (as in the Hayflick and Moorhead work), but rather astrocyte precursors. There are quite a few reports of precursor cells being able to proliferate for long periods of time in defined medium containing growth factors. For example, Reynolds and Weiss (Science 255, 1707-1710, 1992) could grow a neural precursor cell for long periods in EGF. Bogler et al. (PNAS 87 6368-6372, 1990) were able to grow oligodendrocyte-type-2 astrocyte progenitor cells in PDGF and FGF for many months. I have often wondered what results the Barnes group would have obtained if they had removed the nervous tissue from their mouse embryos. To my knowledge, there is no data to suggest that fibroblasts can be cultivated in defined medium indefinitely without transformation - although I would be happy to be proved wrong. I hope this is helpful. Andy -- Andy Groves Division of Biology, 216-76 California Institute of Technology From owner-ageing@net.bio.net Mon Apr 25 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!EU.net!uknet!daresbury!not-for-mail From: Sydney Shall Newsgroups: bionet.molbio.ageing Subject: Infinite growth of normal cells in serum-free medium Date: 26 Apr 1994 09:46:09 +0100 Lines: 66 Sender: daemon@mserv1.dl.ac.uk Distribution: bionet Message-ID: <2pikch$nbo@mserv1.dl.ac.uk> Original-To: ageing@dl.ac.uk There has been a query from Leonid Gavrilov concerning the report from D. W. Barnes et al., that mouse cells have an infinite life span in serum free medium. I understand that the Barnes group have found that this observation can not be reproduced with human cells. Clearly therefore the limit on life-span of normal human cells ( the Hayflick phenomenon still exists). The explanation for the obsevation made by Barnes et al., is still unclear. I am not aware of any published report confirming the observation. Also as another correspondent has already written, it is unclear whether the observation is explained by the absence of serum or by the presence of particular cell types in the Barnes experiments. It amy be that they were looking at cell types which differentiate in the presence of certain growth factors. A more interesting possibility to me, is that in the Barnes' experiment there were primordial embryonic cells which are in fact immortal. This is a distinct theorectical possibility because we know that germ cells are really immortal. It is also possible that the observation made by the Barnes' group was due, as they suggested, to the presence of serum. It is worth considering that the very long term presence of either stimulatory or inhibitory growth factors is part of the molecular causation of the limited life-span. I think this is unlikely because the life-span number is both tissue and species specific, and this implies a genetic origin to me. Moreover, and more convincingly, we have shown that in the human progeria disease, Werner's syndrome, which is a single Mendelian locus disease, there is a change in the length of the life-span of human fibroblasts. A repetition of the observation made by Barnes and coworkers and an exploration of this result would be very worthwhile. ************************************************************************** ************************************************************************** Sydney SHALL, Laboratory of Cell and Molecular Biology, Biology Building, University of Sussex, Brighton, East Sussex BN1 9QG, ENGLAND. Telephone: +44.273.67.83.03 FAX: +44.273.67.84.33 E-Mail: Janet: S.Shall@uk.ac.sussex Elsewhere: S.Shall@sussex.ac.uk EARN/BITNET: S.Shall%sussex@ukacrl ******************************************************************************* ******************************************************************************* From owner-ageing@net.bio.net Tue Apr 26 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!EU.net!sunic!ugle.unit.no!trane.uninett.no!daresbury!not-for-mail From: "Leonid Gavrilov" Newsgroups: bionet.molbio.ageing Subject: JOIN OUR RESEARCH TEAM ! Date: 27 Apr 1994 12:28:54 +0100 Organization: A.N.Belozersky Institute, Moscow State University Lines: 74 Sender: daemon@mserv1.dl.ac.uk Distribution: bionet Message-ID: <2pli9m$j0u@mserv1.dl.ac.uk> X-Russian: Cyrillic Original-To: AGEING@dl.AC.UK April 27, 1994 Dear AGEING LIST subscribers ! The purpose of this message is to invite you to join a research team for receiving EC grant called COPERNICUS. In order to receive this grant a team of 5 researchers from 5 different countries should be created: 2 researchers from EC countries, 2 researchers from Eastern European countries and 1 researcher from the countries of the former Soviet Union (myself). Additional researchers from other eligible countries are very much wellcome. The suggested topic of the grant is: PARENTAL AGE AND OFFSPRING LONGEVITY The members of the team are: 1) Professor F.A. Lints Unite de Genetique Universite catholique de Louvain Place Croix-du-Sud, 2 bte 14 B-1348 Louvain-la-Neuve Belgique tel 32-10-47.36.67 fax 32-10-47.30.31 2) Dr. Leonid A.Gavrilov, Ph.D. A.N.Belozersky Institute Moscow State University 119899 Moscow, RUSSIA FAX: 7 (095) 939-0338 or 7 (095) 939-3181 tel.: 7 (095) 427-0047 3) Dr. Eric Lebourg (Toulouse, France) Thus, we should find 2 additional partners from 2 different countries of the Eastern Europe: Albania, Bulgaria, Checkia, Estonia, Hungary, Latvia, Lithuania, Poland, Romania, Slovakia and Slovenia. WE ARE KINDLY INVITING RESEARCHERS FROM THE ABOVE MENTIONED COUNTRIES TO CONTACT US (BOTH ME AND PROFESSOR LINTS) AS SOON AS POSSIBLE ! The deadline for application is May 2, 1994. So, we are to be very rapid ! Sincerely yours, Dr.Leonid A.Gavrilov, Ph.D. Member of the New York Academy of Sciences P.S.: My CV is available upon the request or could be extracted from archives of AGEING LIST (message 447 of April 23). -- ________________________________________________________________ -- Dr. Leonid A. Gavrilov, Ph.D. Phone: 7 (095) 427 0047 Principal Research Scientist FAX: 7 (095) 939 0338 or A.N.Belozersky Institute 7 (095) 939 3181 Moscow State University E-mail addresses: Moscow 119899 gavrilov@aeiveos.uucp.free.msk.su Russia libro@genebee.msu.su aeiveos@glas.apc.org Please send your answer to my most reliable E-mail address: aeiveos@glas.apc.org if you wish to be sure to reach me. Thank you ! ___________________________________________________________________ From owner-ageing@net.bio.net Tue Apr 26 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!noc.near.net!das-news.harvard.edu!husc-news.harvard.edu!husc.harvard.edu!husc8.harvard.edu!gwmartin Newsgroups: bionet.molbio.ageing Subject: Re: Telomerase Message-ID: <2plpkd$p12@scunix2.harvard.edu> From: gwmartin@husc8.harvard.edu (glover martin) Date: 27 Apr 1994 13:34:05 GMT References: <2pg1c8$kbu@infa.central.susx.ac.uk> Organization: Harvard University, Cambridge, Massachusetts NNTP-Posting-Host: husc8.harvard.edu X-Newsreader: TIN [version 1.2 PL1] Lines: 45 Sydney Shall (bafa1@central.susx.ac.uk) wrote: : The enzyme telomerase has been shown to be present in immortal : cell lines. However, it has never been purified and therefore it has : never been cloned. Yeast telomeres were first cloned in 1982 (Szostak & Blackburn; Cell 29:245). Tetrahymena telomerase was cloned in 1987 (Greider & Blackburn; Cell 51: 887) and I suspect a mammalian homologue has since been cloned. Telomerase turns out to be a protein which contains a small RNA molecule, used to prime telomere synthesis. : The average length of telomeres in dividing normal, : untransformed cells has been shown to shorten as the cell culture ages. True. And Lundblad & Szostak (1989) showed that in yeast, defects in telomere elongation correlates with senescence. : That is, there is a correlation between the length of the average : telomere size and the age of the culture. : The predictions of what the re-introduction of a telomerase : enzyme might do in ageing cells requires the cloning of the gene for : this enzyme. This is proving a very difficult task. : In presence it should be possible to reactivate the expression : of this gene in normal cells, and to repress its expression in cancer : cells, but this also awaits the cloning of the gene. But, only actively dividing cells would really have a need for telomerase, right? I don't know how many cell types, besides epithelial and hematopoietic cells, aren't terminally differentiated in adults. However, part of the problem with transformed cells is that they do not senesce (sp?), like you imply. But I wonder if maintenace of telomere integrity isn't part of some greater cellular aging program. In that case, turning on telomerase in cancer cells may not have the desired effect. : This area is therefore both very active and very excitibg, and : there is lots of room for more people in this field. I agree. Trei From owner-ageing@net.bio.net Tue Apr 26 23:00:00 1994 Path: biosci!daresbury!not-for-mail From: "Leonid Gavrilov" Newsgroups: bionet.molbio.ageing Subject: Parental Age and Offspring Longevity Date: 27 Apr 1994 18:03:39 +0100 Organization: A.N.Belozersky Institute, Moscow State University Lines: 74 Sender: daemon@mserv1.dl.ac.uk Distribution: bionet Message-ID: <2pm5tb$p7n@mserv1.dl.ac.uk> X-Russian: Cyrillic Original-To: AGEING@dl.AC.UK April 27, 1994 Dear AGEING LIST subscribers ! The purpose of this message is to invite you to join a research team for receiving EC grant called COPERNICUS. In order to receive this grant a team of 5 researchers from 5 different countries should be created: 2 researchers from EC countries, 2 researchers from Eastern European countries and 1 researcher from the countries of the former Soviet Union (myself). Additional researchers from other eligible countries are very much wellcome. The suggested topic of the grant is: PARENTAL AGE AND OFFSPRING LONGEVITY The members of the team are: 1) Professor F.A. Lints Unite de Genetique Universite catholique de Louvain Place Croix-du-Sud, 2 bte 14 B-1348 Louvain-la-Neuve Belgique tel 32-10-47.36.67 fax 32-10-47.30.31 2) Dr. Leonid A.Gavrilov, Ph.D. A.N.Belozersky Institute Moscow State University 119899 Moscow, RUSSIA FAX: 7 (095) 939-0338 or 7 (095) 939-3181 tel.: 7 (095) 427-0047 3) Dr. Eric Lebourg (Toulouse, France) Thus, we should find 2 additional partners from 2 different countries of the Eastern Europe: Albania, Bulgaria, Checkia, Estonia, Hungary, Latvia, Lithuania, Poland, Romania, Slovakia and Slovenia. WE ARE KINDLY INVITING RESEARCHERS FROM THE ABOVE MENTIONED COUNTRIES TO CONTACT US (BOTH ME AND PROFESSOR LINTS) AS SOON AS POSSIBLE ! The deadline for application is May 2, 1994. So, we are to be very rapid ! Sincerely yours, Dr.Leonid A.Gavrilov, Ph.D. Member of the New York Academy of Sciences P.S.: My CV is available upon the request or could be extracted from archives of AGEING LIST (message 447 of April 23). -- ________________________________________________________________ -- Dr. Leonid A. Gavrilov, Ph.D. Phone: 7 (095) 427 0047 Principal Research Scientist FAX: 7 (095) 939 0338 or A.N.Belozersky Institute 7 (095) 939 3181 Moscow State University E-mail addresses: Moscow 119899 gavrilov@aeiveos.uucp.free.msk.su Russia libro@genebee.msu.su aeiveos@glas.apc.org Please send your answer to my most reliable E-mail address: aeiveos@glas.apc.org if you wish to be sure to reach me. Thank you ! ___________________________________________________________________ From owner-ageing@net.bio.net Tue Apr 26 23:00:00 1994 Path: biosci!agate!ihnp4.ucsd.edu!swrinde!cs.utexas.edu!howland.reston.ans.net!pipex!uknet!daresbury!not-for-mail From: "Leonid Gavrilov" Newsgroups: bionet.molbio.ageing Subject: Grant for Eastern European scientists Date: 27 Apr 1994 18:07:19 +0100 Organization: A.N.Belozersky Institute, Moscow State University Lines: 74 Sender: daemon@mserv1.dl.ac.uk Distribution: bionet Message-ID: <2pm647$pd5@mserv1.dl.ac.uk> X-Russian: Cyrillic Original-To: AGEING@dl.AC.UK April 27, 1994 Dear AGEING LIST subscribers ! The purpose of this message is to invite you to join a research team for receiving EC grant called COPERNICUS. In order to receive this grant a team of 5 researchers from 5 different countries should be created: 2 researchers from EC countries, 2 researchers from Eastern European countries and 1 researcher from the countries of the former Soviet Union (myself). Additional researchers from other eligible countries are very much wellcome. The suggested topic of the grant is: PARENTAL AGE AND OFFSPRING LONGEVITY The members of the team are: 1) Professor F.A. Lints Unite de Genetique Universite catholique de Louvain Place Croix-du-Sud, 2 bte 14 B-1348 Louvain-la-Neuve Belgique tel 32-10-47.36.67 fax 32-10-47.30.31 2) Dr. Leonid A.Gavrilov, Ph.D. A.N.Belozersky Institute Moscow State University 119899 Moscow, RUSSIA FAX: 7 (095) 939-0338 or 7 (095) 939-3181 tel.: 7 (095) 427-0047 3) Dr. Eric Lebourg (Toulouse, France) Thus, we should find 2 additional partners from 2 different countries of the Eastern Europe: Albania, Bulgaria, Checkia, Estonia, Hungary, Latvia, Lithuania, Poland, Romania, Slovakia and Slovenia. WE ARE KINDLY INVITING RESEARCHERS FROM THE ABOVE MENTIONED COUNTRIES TO CONTACT US (BOTH ME AND PROFESSOR LINTS) AS SOON AS POSSIBLE ! The deadline for application is May 2, 1994. So, we are to be very rapid ! Sincerely yours, Dr.Leonid A.Gavrilov, Ph.D. Member of the New York Academy of Sciences P.S.: My CV is available upon the request or could be extracted from archives of AGEING LIST (message 447 of April 23). -- ________________________________________________________________ -- Dr. Leonid A. Gavrilov, Ph.D. Phone: 7 (095) 427 0047 Principal Research Scientist FAX: 7 (095) 939 0338 or A.N.Belozersky Institute 7 (095) 939 3181 Moscow State University E-mail addresses: Moscow 119899 gavrilov@aeiveos.uucp.free.msk.su Russia libro@genebee.msu.su aeiveos@glas.apc.org Please send your answer to my most reliable E-mail address: aeiveos@glas.apc.org if you wish to be sure to reach me. Thank you ! ___________________________________________________________________ From owner-ageing@net.bio.net Fri Apr 29 23:00:00 1994 Path: biosci!agate!howland.reston.ans.net!EU.net!uknet!daresbury!not-for-mail From: "Leonid Gavrilov" Newsgroups: bionet.molbio.ageing Subject: Funds for Eastern European scientists Date: 30 Apr 1994 11:25:43 +0100 Organization: A.N.Belozersky Institute, Moscow State University Lines: 358 Sender: daemon@mserv1.dl.ac.uk Distribution: bionet Message-ID: <2ptbn7$7st@mserv1.dl.ac.uk> X-Russian: Cyrillic Original-To: AGEING@dl.AC.UK April 30, 1994 Dear subscribers, European Community allocated funds for international scientific collaboration of European scientists (program called COPERNICUS). I am one of the coordinators of the research team of 5 scientists from 5 different European countries that will work on the project: PARENTAL AGE AND OFFSPRING LONGEVITY We have room in our research group for scientists from the following countries: Albania, Bulgaria, Checkia, Estonia, Latvia, Lithuania, Poland, Romania, Slovakia and Slovenia We are kindly inviting the scientists from the above mentioned countries to join our research team and to send their CVs before the COPERNICUS deadline (May 2) to my E-mail address: aeiveos@glas.apc.org Thank you in advance for your kindness. Sincerely yours, Dr.Leonid A.Gavrilov, Ph.D. Member of the New York Academy of Sciences P.S.: My CV is printed below for introduction. *************************************************************************** April 24, 1994 C U R R I C U L U M V I T A E Dr. Leonid A. Gavrilov, Ph.D. Phone: 7 (095) 427 0047 Principal Research Scientist FAX: 7 (095) 939 0338 or A.N.Belozersky Institute 7 (095) 939 3181 Moscow State University E-mail addresses: Moscow 119899 libro@genebee.msu.su Russia gavrilov@aeiveos.uucp.free.msk.su aeiveos@glas.apc.org Date of Birth: August 28, 1954 Place of Birth: USSR (Russia), town of Sverdlovsk (now Ekaterinburg) Research interests: Mechanisms of ageing, age-related mortality, longevity. Education: Ph.D. - Moscow State University (1980) in mortality kinetics in human populations and animal models, with emphasis on mathematical models of mortality. M.Sc. - Moscow State University (1976) in mechanisms of aging with emphasis on mathematical models of aging Professional Experience: 1991-Present Principal Research Scientist, A.N.Belozersky Institute, Moscow State University. 1987-1991 Senior Research Scientist, A.N.Belozersky Institute, Moscow State University. 1980-1987 Research Scientist, A.N.Belozersky Institute, Moscow State University. Professional Activities and Other Positions: - Member of the New York Academy of Science (Member ID #364586) - Principal Investigator of the International Science Foundation Research Grant # M7E000. - Chairman of the Subsection of the Biology of Life Span at Moscow Society of Naturalists (1981-1992). - Expert (book reviewer) for journals AGE AND AGEING, AGEING AND SOCIETY, J.GENERAL BIOLOGY and Publishers "MIR". - Expert (peer reviewer) for journals MATHEMATICAL POPULATION STUDIES, J.GENERAL BIOLOGY. - Chairperson of the Biological Session at the II European Congress of Gerontology (Madrid, 1991). Certificate signed by F.Guillen Llera, General Secretary of the Congress. - Expert (book editor) for Institute of Scientific Information (VINITI, Moscow). - Fellow of Moscow Society of Naturalists. - Senior Research Scientist at the Russian Academy of Sciences Institute for System Analysis. - Scientific Secretary of Demographic Section of Moscow House of Scientists (1984-1991). - Fellow of the Moscow House of Scientists. Awards: - International Science Foundation (ISF) 1994 Research Grant #M7E000. - International Science Foundation (ISF) 1993 Emergency Grant Award. - International Science Foundation (ISF) 1994 Emergency Grant for Russian Scientists of the Top Category. - The Certificate from the International Association of Gerontology (1993) signed by the President and the Secretary General of the International Association of Gerontology. - A.N.Belozersky Institute 1991 Award for the best scientific research in the Institute in 1990-1991. - Moscow Society of Naturalists 1989 Award for the best scientific research in natural science in 1986-1988. - Grant Award with invitation to European Population Conference (Paris, October 1991). - Financial Award with invitation of giving a lectures to Trinity College, Cambridge University (December, 1991). - Financial Award with invitation to the Annual Meeting of British Biochemical Society (Southampton, April, 1992). Publications: 65 publications, including 5 books. Selected publications in the most prestigeous scientific peer reviewed journals are: - "Sex and Longevity", Nature, 1994, vol.367, No.6463, p.520. - "Fruit Fly Aging and Mortality", Science, 1993, vol.260, No.5114, p.1565. - "When Your Time's Up", British Medical Journal, 1993, vol.306, No.6880, p.796. - "Human Life Span Stopped Increasing: Why?", Gerontology, 1983, vol.29, pp.176-180. - "Life Span is Determined by Resting Metabolic Rate of Parents Only!", Age, 1989, vol.12, p.113. - "Human Species-Specific Life-Span: Its Estimation and Interpretation", Age, 1985, vol.8, p.94. - "A New Trend in Human Mortality Decline: Derectangularization of the Survival Curve", Age, 1985, vol.8, p.93. - "Biology of Aging (Book Review)", Age and Ageing, 1993, vol.22, pp.233-234. - "Evolutionary Biology of Aging (Book Review)", Ageing and Society, 1992, vol.12, No.4, pp.539-541. - "How Many Cell Divisions in 'Old' Cells?", International Journal of Geriatric Psychiatry, 1993, vol.8, No.6, p.528. - "Common Sense and the Limits to Life", International Journal of Geriatric Psychiatry, 1993, vol.8, No.8, p.695. - "Historically Stable Indices of Mortality and their Use for Medical Geography", Geographia Medica, 1984, vol.14, pp.305-306. - "Epidemiologic Approach to the Biology of Human Life Span", Geographia Medica, 1985, vol.15, pp.40-64. The key publication where the main results of the previous studies are summarised is: THE BIOLOGY OF LIFE SPAN: A QUANTITATIVE APPROACH. By Leonid A.Gavrilov and Natalia S.Gavrilova. Harwood Academic Publishers: 1991. Pp. 385, $ 120, ISBN 3-7186-4983-7. Positive reviews of this book were published by the following journals: 1. AGE AND AGEING, 1992, vol.21, No.5, pp.386-387. 2. AGEING AND SOCIETY, 1991, vol.11, No.4, pp.509-510. 3. ARCHIVES OF GERONTOLOGY AND GERIATRICS, 1992, vol.15, No.2, pp.192-194. 4. ARGOMENTI DI GERONTOLOGIA, 1993, vol.5, No.2, p.91. 5. BIOESSAYS, 1993, vol.15, No.5, pp.359-362. 6. BOLLETTINO DELLA SOCIETA ITALIANA DI GERONTOLOGIA E GERIATRIA, 1992, No.7, p.1. 7. BRITISH MEDICAL JOURNAL, 1992, vol.305, No.6850, p.431. 8. EDUCATIONAL GERONTOLOGY, 1993, vol.19, No.1, pp.92-93. 9. EXPERIMENTAL GERONTOLOGY, 1992, vol.27, pp.251-253. 10. FREE RADICAL BIOLOGY & MEDICINE, 1992, vol.12, pp.331-332. 11. GERONTOLOGIST, 1991, vol.31, No.5, p.707. 12. HUMAN BIOLOGY, 1992, vol.64, No.4, pp.630-632. 13. INTERNATIONAL JOURNAL OF GERIATRIC PSYCHIATRY, 1992, vol.7, No.8, p.614. 14. JOURNAL OF CROSS-CULTURAL GERONTOLOGY, 1993, vol.8, No.3, pp.281-290. 15. JOURNAL OF EPIDEMIOLOGY & COMMUNITY HEALTH, 1992, vol.46, No.6, p.630. 16. JOURNAL OF THE INSTITUTE OF ACTUARIES, 1992, vol.119, Part II, pp.379-381. 17. JOURNAL OF ORTHOMOLECULAR MEDICINE, 1993, vol.8, No.1, pp.59-60. 18. NATURE, 1991, vol.352, 29 August, pp.767-768. 19. POPULATION AND DEVELOPMENT REVIEW, 1992, vol.18, No.3, pp.555-558. 20. POPULATION STUDIES, 1992, vol.46, No.2, pp.366-367. 21. QUARTERLY REVIEW OF BIOLOGY, 1993, vol.68, p.92. 22. STATISZTIKAI SZEMLE (STATISTICAL REVIEW, In Hungarian), 1992,vol.70,No.6,pp.546-547. Response of the Leading Scientists to the Scientific Research and the Book by L.A.Gavrilov: "HIGHLY RECOMMENDED FOR STIMULATING FRESH APPROACHES ... " - Professor Roy L.Walford, M.D., UCLA School of Medicine, USA. THE GERONTOLOGIST, 1991, vol.31, No.5, p.707. "CLEARLY, THIS IS A VERY INTERESTING, PROVOCATIVE AND THOUGHT-PROVOKING BOOK" - Dr. Ward Dean, M.D., The Center for Bio-Gerontology, Pencasola, Florida, USA. EXPERIMENTAL GERONTOLOGY, 1992, vol.27, No.2, pp.251-253. "THIS IS AN INTERESTING BOOK, FULL OF TABLES AND DATA AND POLEMICAL IN ITS APPROACH." - Professor William A.Pryor, Director of Biodynamic Institute, Louisiana State University, Baton Rouge, LA,USA. FREE RADICAL BIOLOGY & MEDICINE, 1992, vol.12, pp.331-332. "IT BRINGS TO BEAR A FRESH APPROACH WHOSE IDEAS ARE LOGICALLY PRESENTED AND DEVELOPED". - Dr. Brian Merry, Ph.D., Institute of Human Aging, University of Liverpool, United Kingdom. AGEING AND SOCIETY, 1991, vol.11, No.4, pp.509-510. "GOOD NEW BOOK ON AGEING". - Dr. Thomas B.L.Kirkwood, Ph.D. Head of the Laboratory of Mathematical Biology, National Institute for Medical Research, London, United Kingdom. NATURE, 1991, vol.352, 29 August, pp.767-768. "... SHOULD BE READ BY ANYONE SERIOUSLY INTERESTED IN AGEING. ... YOU WILL FIND THIS A SURPRISINGLY GOOD 'READ', AND A MUST FOR EVERY LIBRARY". - Dr. D.Sebastian Fairweather, Physician, Department of Geriatric Medicine, Oxford, United Kingdom. AGE AND AGEING, 1992, vol.21, No.5, pp.386-387. "THE BOOK FEATURES QUITE A BIT OF DEMOGRAPHIC THEORY AND ANALYSIS. .... IT IS REFRESHING" - Professor Michael R.Rose, University of California, Irvine, USA. ANNALS OF HUMAN BIOLOGY, 1992, vol.19, No.3, pp.320-321. "THE GAVRILOVS HAVE MADE A VALUABLE CONTRIBUTION TO THE STUDY OF LIFE SPAN, AND ANY FUTURE WORK ON THE SUBJECT WILL HAVE TO TAKE ACCOUNT OF THE RICH CONTENTS OF THIS BOOK". - Dr. Vaino Kannisto, Former United Nations Adviser on Demography POPULATION STUDIES, 1992, vol.46, No.2, pp. 366-367. "EIN AKTUELLES BUCH, DAS SICHERLICH DIE INTERNATIONALE DISKUSSION DES PROBLEMS BEFORDERN KANN." - Prof. Dr. Ingeborg Falck, Berlin, Germany. ZEITSCHRIFT FUR GERONTOLOGIE, 1991, Band 25, Heft 1, S.56. "THE BOOK IS WELL WRITTEN, EASY TO READ, AND SHOULD BE OF INTEREST TO A WIDE SPECTRUM OF READERS INCLUDING PHYSICIANS, BIOLOGISTS, BIOCHEMISTS, GERONTOLOGISTS..." - Prof. Robert W.Gracy, University of North Texas, USA. EDUCATIONAL GERONTOLOGY, 1993, vol.19, No.1, pp.92-93. "... THIS BOOK IS A HIGHLY VALUABLE CONTRIBUTION TO THE DISCIPLINE. ...THIS BOOK SHOULD MAKE SCIENTISTS TAKE NOTICE OF RESEARCH ON AGING AND LONGEVITY ... IN THE FORMER SOVIET UNION" - Dr. S.Jay Olshansky, Ph.D., University of Chicago, USA POPULATION AND DEVELOPMENT REVIEW, 1992, vol.18, No.3, pp.555-558. "... MANY WILL FIND AN EXCURSION INTO IT STIMULATING". - Dr. Emily Grundy, Lecturer in Gerontology, Age Concern Institute of Gerontology, King's College, London, UK. BRITISH MEDICAL JOURNAL, 1992, vol.305, No.6850, p.431. "GAVRILOV MAKES THE BEST ATTEMPT I KNOW OF TO DISTINGUISH HOW LONG PEOPLE COULD LIVE FROM HOW LONG THEY ACTUALLY LIVE - ONE OF THE MORE DIFFICULT TASKS FOR BOTH BIOLOGY AND STATISTICS... GAVRILOV'S SCHOLARSHIP IS IMPRESSIVE". - Professor Nathan Keyfitz, International Institute for Applied Systems Analysis, Laxenburg, Austria. MATHEMATICAL POPULATION STUDIES, 1991, vol.3, No.2, p.161. "LEONID GAVRILOV IS A TOP-FLIGHT STATISTICAL POPULATION BIOLOGIST... THE AREA IS IMPORTANT WELL BEYOND THE CONFINES OF BIOLOGICAL GERONTOLOGY AND EXTENDS INTO POLITICS AND SOCIAL PLANNING. GAVRILOV IS VERY GOOD ON JUST THIS SORT OF THING. HE HAS A FRESH APPROACH, NEW IDEAS, A GREAT DEAL OF DATA NOT READILY AVAILABLE ELSEWHERE." - Roy. L. Walford, Professor of Pathology, UCLA School of Medicine, USA. MATHEMATICAL POPULATION STUDIES, 1991, vol.3, No.2, p.161. "THIS BOOK IS DIRECTED TOWARD RESEARCHERS INTERESTED IN STATISTICAL PATTERNS AND MATHEMATICAL MODELS OF HUMAN LIFE SPAN. FOR STUDENTS WITHIN THIS DEFINED AREA OF RESEARCH, THIS TEXT OFFERS A GOOD HISTORICAL PERSPECTIVE, INSIGHT INTO THE RUSSIAN LITERATURE, AND INSIGHT INTO THE QUANTITATIVE ANALYSIS OF LIFE SPAN" - Dr. Deborah A.Roach, Department of Zoology, Duke University, Durham, USA. ECOLOGY, 1992, vol.73, No.1, pp.379-381. "THIS BOOK IS IN ESSENCE A BIOSTATISTICAL ANALYSIS OF THE PROBLEMS OF LIFESPAN, HUMAN AND ANIMAL. ... THE LITERATURE IN MANY LANGUAGES HAD CLEARLY BEEN WELL COVERED, AND MUCH HAS BEEN MADE OF NATIONAL AND OTHER MORTALITY STATISTICS IN THIS CENTURY. ... A USEFUL SOURCE BOOK." - Dr. F.I.Caird, D.M., F.R.C.P., Professor of Geriatric Medicine, Southern General Hospital, Glasgow, UK. J. OF CLINICAL AND EXPERIMENTAL GERONTOLOGY, 1992, vol.14, No. 3 & 4, p.309. "... THE READER WILL BENEFIT ENORMOUSLY FROM THE BROADENING OF PERSPECTIVE AS A RESULT OF EXPOSURE TO THE GAVRILOVS' THEORIES ON THE BIOLOGY OF LIFE SPAN" - Dr. M. Michael Akiyama, University of Michigan, Dearborn, USA. HUMAN BIOLOGY, 1992, vol.64, No.4, pp.630-632. "THIS IS A PROVOCATIVE AND REFRESHING BOOK" - Dr.Alan R.Hipkiss, Age Concern Institute of Gerontology, King's College London, UK. INTERNATIONAL JOURNAL OF GERIATRIC PSYCHIATRY, 1992, vol.7,No.8,p.614. "THIS BOOK SHOULD BE READ BY ALL MEMBERS OF THE PROFESSION INTERESTED IN MORTALITY - WHICH SHOULD BE ALL MEMBERS OF THE PROFESSION" - Dr. H.A.R.Barnett, Institute of Actuaries, UK. J.OF THE INSTITUTE OF ACTUARIES, 1992, vol.119, Part II, pp.379-381. "TO SUM UP, THE BOOK IS SUBSTANTIAL, INTERESTING AND BRINGS FRESH AIR. WE CAN CONFIDENTLY COMMEND ITS STUDY TO ALL SCIENTISTS ... " - Dr. Janos Izsak, Department of Zoology, Berzsenyi Teachers' College, Szombathely, Hungary. ARCHIVES OF GERONTOLOGY AND GERIATRICS, 1992, vol.15, No.2,pp.192-194. "A GENERAL THEORY OF LIFESPAN IS THUS CREATED." - - Dr. Norman Vetter, University Hospital of Wales, Cardiff, UK. J.OF EPIDEMIOLOGY & COMMUNITY HEALTH, 1992, vol.46, No.6, p.630. "I FOUND IT TO BE HIGHLY REWARDING READING." - Dr. Edward J.Masoro, University of Texas Health Science Center. QUARTERLY REVIEW OF BIOLOGY, 1993, vol.68, p.92. "THIS ... IS AN EXCELLENT BOOK. ... NOBODY INTERESTED IN AGING SHOULD FAIL TO READ". - Dr. C.S.Downes, Department of Zoology, University of Cambridge, UK. BIOESSAYS, 1993, vol.15, No.5, pp.359-362. "...PROVOCATIVE AND ENTERTAINING READING AND CAN BE RECOMMENDED TO ANYONE WITH AN INTEREST IN BIOLOGICAL AGING." - Dr. Douglas E.Crews, Department of Anthropology, Ohio State University, USA JOURNAL OF CROSS-CULTURAL GERONTOLOGY, 1993, vol.8, No.3, pp.281-290. "IT DESERVES TO BE READ WIDELY. SCIENTISTS AND PHYSICIANS WHO ARE INTERESTED IN THE AGING OF POPULATIONS OR OF INDIVIDUALS WILL BE MUCH MORE EFFECTIVE IN THEIR WORK IF THEY BECOME FAMILIAR WITH THE SUBJECT MATTER OF THIS BOOK." - JOURNAL OF ORTHOMOLECULAR MEDICINE, 1993, vol.8, No.1, pp.59-60. *********************************THE END****************************** -- ________________________________________________________________ -- Dr. Leonid A. Gavrilov, Ph.D. Phone: 7 (095) 427 0047 Principal Research Scientist FAX: 7 (095) 939 0338 or A.N.Belozersky Institute 7 (095) 939 3181 Moscow State University E-mail addresses: Moscow 119899 gavrilov@aeiveos.uucp.free.msk.su Russia libro@genebee.msu.su aeiveos@glas.apc.org Please send your answer to my most reliable E-mail address: aeiveos@glas.apc.org if you wish to be sure to reach me. Thank you ! ___________________________________________________________________ From owner-ageing@net.bio.net Sat Apr 30 23:00:00 1994 Path: biosci!internet!biosci!not-for-mail From: kristoff (David Kristofferson) Newsgroups: bionet.molbio.ageing Subject: UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ Date: 1 May 1994 02:00:25 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 275 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <199405010900.CAA19069@net.bio.net> NNTP-Posting-Host: net.bio.net Four important items follow: How to cancel e-mail subscriptions to BIOSCI newsgroups, BIOSCI archive searching, the BIOSCI FAQ, and the BIOSCI User Address Directory form. If you have not yet listed yourself in our BIOSCI user directory, please take a few minutes to complete and return the form below. If your personal information has changed since you listed yourself, please send us an updated form. 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