From owner-ageing@net.bio.net Fri Sep 01 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!tank.news.pipex.net!pipex!dispatch.news.demon.net!demon!sunsite.doc.ic.ac.uk!lyra.csx.cam.ac.uk!news From: Jong Newsgroups: bionet.molbio.ageing Subject: (no subject) Date: 2 Sep 1995 09:57:06 GMT Organization: MRC Lines: 14 Message-ID: <4299pi$fes@lyra.csx.cam.ac.uk> NNTP-Posting-Host: sonja.acad.cai.cam.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2b4 (Windows; I; 16bit) Dear, I have made a page on the abstracts of Ginseng related scientific research. It is on; http://sonja.acad.cai.cam.ac.uk/aging/ginseng/ginseng.htm If you know any more interesting things on it, please let me know. cheers, Jong From owner-ageing@net.bio.net Sat Sep 02 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!news.uoregon.edu!news.u.washington.edu!root From: Lance Stewart Newsgroups: bionet.molbio.ageing Subject: (no subject) Date: 3 Sep 1995 04:56:53 GMT Organization: university of washington Lines: 4 Message-ID: <42bcil$m30@nntp3.u.washington.edu> NNTP-Posting-Host: cs103-16.u.washington.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:bionet.molbio.ageing Does Geron corp. have a Homepage? How can I invest in this exciting company? Lance. From owner-ageing@net.bio.net Sat Sep 02 23:00:00 1995 Path: biosci!agate!sunsite.doc.ic.ac.uk!dispatch.news.demon.net!demon!btnet!newsfeed.internetmci.com!news.uoregon.edu!vixen.cso.uiuc.edu!news.ecn.bgu.edu!psuvax1!news.eecs.nwu.edu!newsfeed.acns.nwu.edu!news.cc.uic.edu!E1.ED.UIC.EDU!u56375 From: u56375@uicvm.uic.edu (Dr. R. Krishnaraj) Newsgroups: bionet.molbio.ageing Subject: Post-Doc Position Date: Thu, 31 Aug 1995 21:38:50 Organization: University of Illinois at Chicago Lines: 10 Message-ID: NNTP-Posting-Host: e1.ed.uic.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] POSTDOCTORAL RESEARCH ASSOCIATESHIP available immediately for a Ph.D or equivalent with experience in molecular immunology (flow cytometry, quantitative, PCR, cytokines, etc.) to study human NK cells (NIH funded). Send curriculum vitae, reprints and three reference letters to: Dr. R. Krishnaraj, University of Illinois at Chicago, Department of Medicine (M/C 787), 840 South Wood Street, Chicago, IL 60612. FAX 312-413-2693. -- Educational Technology Lab University of Illinois at Chicago E-Mail: u29440@uicvm.uic.edu From owner-ageing@net.bio.net Sat Sep 02 23:00:00 1995 Path: biosci!agate!newsxfer.itd.umich.edu!newsfeed.internetmci.com!news.uoregon.edu!vixen.cso.uiuc.edu!uwm.edu!psuvax1!news.eecs.nwu.edu!newsfeed.acns.nwu.edu!news.cc.uic.edu!E1.ED.UIC.EDU!u56375 From: u56375@uicvm.uic.edu (Dr. R. Krishnaraj) Newsgroups: bionet.molbio.ageing Subject: Post-Doc position or Research specialist Date: Thu, 31 Aug 1995 21:51:51 Organization: University of Illinois at Chicago Lines: 18 Message-ID: NNTP-Posting-Host: e1.ed.uic.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] POSTDOCTORAL RESEARCH ASSOCIATESHIP avaialable immediately with experience in molecular immunology (flow cytometry, quantitative PCR, cytokines, etc.) to study human NK cells (NIH funded). Send curriculum vitae, reprints and three reference letters to: Dr. R. Krishnaraj, University of Illinois at Chicago, Department of Medicine (M/C 787), 840 South Wood Street, Chicago, IL 60612. FAX 312-413-2693. RESEARCH SPECIALIST: Part-time (or full-time, if funds are available) Research Specialist: BS or MS with some experience in immunology tissue culture and molecular biology techniques to work on human blood samples. Available immediately. Send resume to Dr. Krishnaraj, Geriatric Medicine (M/C 787) University of Illinois, 840 S. Wood St, Chicago, IL 60612. Fax 312- 413-2693. -- Educational Technology Lab University of Illinois at Chicago E-Mail: u29440@uicvm.uic.edu From owner-ageing@net.bio.net Sun Sep 03 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!usc!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!news.crd.ge.com!rebecca!gk1702 From: gk1702@csc.albany.edu (KRISHNA GOPALA S) Newsgroups: bionet.molbio.ageing Subject: situation wanted Date: 4 Sep 1995 03:03:20 GMT Organization: The University at Albany Lines: 214 Distribution: usa Message-ID: <42dq9o$85a@rebecca.albany.edu> NNTP-Posting-Host: eve.albany.edu RESUME From Mr.S.Gopal 248 Shakerrun Apts. Albany, NY-12205. Tel:(518)-456-1604 sub: application for electron microscopist- cum-computer programmer. EDUCATION: 1992-1993 : Certificate in electron microscopy technician from V.A.Hospital, Albany, NY. 1987-1990 : M.S. in Biology from Meerut University, India. 1985-1990 : Certificate in computer programming from M.K. University, India. Experience on basic, cobol, fortran, dbase-iii, dbase-iv, wordperfect, lotus-123, turbo prolog, pl/1, c, c++, visual basic, assembly language, database management, systems analysis & design, management information systems, business data processing, pascal, turbo-pascal, excel, image processing, operating systems like dos, unix, vax etc. EXPERIENCE: 1993-Continuing : Electron microscopist-cum-computer programmer at NYS Dept of health, Albany, NY. Duties: writing scientific programs for 3-D image analysis by spider and web software. Specimen preparation, processing for Trans- mission, Scanning, Cryo-Electron Microscopes, ultramicrotomy, positive , negative staining techniqs, operation, maintainance of microscopes, photography techniques, elemental analysis and image analysis. From owner-ageing@net.bio.net Sun Sep 03 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!usc!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!news.crd.ge.com!rebecca!gk1702 From: gk1702@csc.albany.edu (KRISHNA GOPALA S) Newsgroups: bionet.molbio.ageing Subject: situation wanted Date: 4 Sep 1995 03:02:32 GMT Organization: The University at Albany Lines: 26 Distribution: usa Message-ID: <42dq88$858@rebecca.albany.edu> NNTP-Posting-Host: eve.albany.edu Newsgroups: capdist.general from: gopala s.krishna subject: electron microscopist position-situation wanted summary: resume Followup-To:anywhere in usa Distribution: usa Organization: The University at Albany Cc: Keywords: situation wanted SITUATION WANTED B.S, M.S, Master Of philosophy in Biology, certificate in Electron Microscopy from V.A.Hospital, Albany, NY. Two years experience on Transmission, Scanning, Cryo-Electron microscopes, elemental analysis and image analysis. Available immediately. Willing to relocate. Call (518)-456-1604 or write to: Mr.S.Gopal 248 Shakerrun Apts. Albany, NY-12205. (518)-456-1604 From owner-ageing@net.bio.net Sun Sep 03 23:00:00 1995 Path: biosci!TENET.EDU!dashley From: dashley@TENET.EDU (Don Ashley) Newsgroups: bionet.molbio.ageing Subject: Investing In Geron Date: 3 Sep 1995 20:09:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <42bcil$m30@nntp3.u.washington.edu> NNTP-Posting-Host: net.bio.net Let me know when you find out. I think Japan paid Geron $20 million recently for rights to market their cancer arresting procedures. On 3 Sep 1995, Lance Stewart wrote: > Does Geron corp. have a Homepage? How can I invest in this exciting > company? Lance. > > > > From owner-ageing@net.bio.net Mon Sep 04 23:00:00 1995 Path: biosci!demo.ucl.ac.be!wunsch From: wunsch@demo.ucl.ac.be (WUNSCH Guillaume) Newsgroups: bionet.molbio.ageing Subject: Seminars on Longevity Research Date: 5 Sep 1995 05:12:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 67 Sender: daemon@net.bio.net Distribution: world Message-ID: <9509051208.AA20828@sci1.sri.ucl.ac.be> NNTP-Posting-Host: net.bio.net Dear Colleagues, The purpose of this message is to inform you about the significant extension of the list of our scientific lectures and seminars on longevity research after our 3 recent presentations on the III European Congress of Gerontology in Amsterdam and a seminar at the University of Utrecht. The new updated schedule of our seminars is printed below. We would be happy to give a seminar in your organization too (especially if you are located in Europe) and to discuss the opportunities for scientific collaboration on longevity research and centenarian studies. Sincerely yours, Dr.Leonid A.Gavrilov, Ph.D. Dr.Natalia S.Gavrilova, Ph.D. P.S.: The updated extended list of our seminars is printed below. ****************************************************************** OUR SCHEDULE OF LECTURES AND SEMINARS ON LONGEVITY RESEARCH: 1. Belgium. September 5-13, 15-20. Contact person: Professor Guillaume Wunsch. Phone: (32 10) 47 29 51 Fax: (32 10) 47 29 52 Working Address: Dr.Leonid A.Gavrilov, Ph.D. c/o Prof.G.Wunsch Institut de demographie place Montesquieu, 1/17 B-1348 Louvain-la-Neuve Belgium Home Address: Dr.Leonid A.Gavrilov, Ph.D. c/o Mrs.Cecile Goossens Clos des Molons, 1 B-1348 Louvain-la-Neuve Belgium Home phone: (32 10) 45 07 67 2. United Kingdom. September 13-14 and perhaps later. Contact person: Dr. Michael Murphy. Phone: 0171-405 7686 Fax: 0171-955 6833 Address: Dr.Leonid A.Gavrilov,Ph.D. c/o Dr.M.Murphy Population Studies London School of Economics Houghton Street London WC2A 2AE UK 3. France. September 21-22 and perhaps later. Contact person: Dr.Michel Allard. Phone: 33(1) 44 96 10 95 Fax: 33(1) 44 96 11 99 Address: Dr. Leonid A.Gavrilov,Ph.D. c/o Dr.Michel Allard IPSEN Foundation 24, rue Erlanger 75781 PARIS cedex 16 France P.S.: This list might be extended in future. 9 From owner-ageing@net.bio.net Tue Sep 05 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!news.sprintlink.net!in2.uu.net!comp.vuw.ac.nz!waikato!auckland.ac.nz!kcbbs!planet!chambers!steve From: steve@chambers.ak.planet.co.nz (Steve Chambers) Newsgroups: bionet.molbio.ageing,sci.life-extension Subject: Re: solid data that says cancer caused by ageing process (Forwarded article) Message-ID: Date: Wed, 6 Sep 95 17:15:24 +1200 References: Organization: PlaNet (Auckland New Zealand) Lines: 33 Xref: biosci bionet.molbio.ageing:1940 sci.life-extension:7050 In grovesa@starbase1.caltech.edu (Andrew K. Groves) writes: >In article , >steve@chambers.ak.planet.co.nz (Steve Chambers) wrote: >> Let's tidy this discussion up by reframing it. I say that if you >> succeed in preventing aging you'll also have eliminated cancer. I'm >> keen for anyone to present arguments to the contrary. >An interesting thought - although do you also mean to include childhood >cancers such as neuroblastomas, or embryonal carcinomas? These are >screw-ups right at the outset of life, and whether they are also due to a >manifestation of the ageing process is a moot point. Sure, why not? Just because an aging process is most observable late in life doesn't mean that it hasn't been going on throughout. Adults may not have neuroblasts and other early development cell types but that's not important - it's the somatic mutations (which can happen at any age) that are the "aging" process of interest here. Besides, we're quite happy to conceptualize Werner's and other progerias as premature aging. Why not other age-linked phenomenon that occur in childhood? Steve PS Andy - We're talking about aging of whole organisms here ;-) Good to hear from you again. -- ________________________ (I_lurk,_therefore_I_am!_\ ,,, Steve Chambers (o o) steve@chambers.ak.planet.co.nz -----------------------oOO--(_)--OOo------------------------------------ From owner-ageing@net.bio.net Tue Sep 05 23:00:00 1995 Path: biosci!demo.ucl.ac.be!wunsch From: wunsch@demo.ucl.ac.be (WUNSCH Guillaume) Newsgroups: bionet.molbio.ageing Subject: Funds for Aging Research Date: 6 Sep 1995 04:26:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 73 Sender: daemon@net.bio.net Distribution: world Message-ID: <9509061122.AA00536@sci1.sri.ucl.ac.be> NNTP-Posting-Host: net.bio.net Dear Colleagues, We have just received an information from European Community grant agency that a lot of funds could be received for our research work on aging and longevity on condition of international collaboration. We are looking for scientific partners in order to write joint grant application. Our schedule of the visits to the UK, France and Belgium is printed below (including fax numbers). The scientists interested in participation in our project based on our previous research (described in our book "The Biology of Life Span", Harwood Academic Publisher, 1991) are invited to join us. Sincerely yours, Dr.Leonid A.Gavrilov, Ph.D. Principal Research Scientist, Moscow State University Dr.Natalia S.Gavrilova, Ph.D. Senior Research Scientist Institute for System Analysis, Russian Academy of Sciences P.S.: Our schedule is printed below for your information. ************************************************************* OUR SCHEDULE OF LECTURES AND SEMINARS ON LONGEVITY RESEARCH: 1. Belgium. September 5-13, 15-20. Contact person: Professor Guillaume Wunsch. Phone: (32 10) 47 29 51 Fax: (32 10) 47 29 52 Working Address: Dr.Leonid A.Gavrilov, Ph.D. c/o Prof.G.Wunsch Institut de demographie place Montesquieu, 1/17 B-1348 Louvain-la-Neuve Belgium Home Address: Dr.Leonid A.Gavrilov, Ph.D. c/o Mrs.Cecile Goossens Clos des Molons, 1 B-1348 Louvain-la-Neuve Belgium Home phone: (32 10) 45 07 67 2. United Kingdom. September 13-14 and perhaps later. Contact person: Dr. Michael Murphy. Phone: 0171-405 7686 Fax: 0171-955 6833 Address: Dr.Leonid A.Gavrilov,Ph.D. c/o Dr.M.Murphy Population Studies London School of Economics Houghton Street London WC2A 2AE UK 3. France. September 21-22 and perhaps later. Contact person: Dr.Michel Allard. Phone: 33(1) 44 96 10 95 Fax: 33(1) 44 96 11 99 Address: Dr. Leonid A.Gavrilov,Ph.D. c/o Dr.Michel Allard IPSEN Foundation 24, rue Erlanger 75781 PARIS cedex 16 France P.S.: This list might be extended in future. From owner-ageing@net.bio.net Tue Sep 05 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.larc.nasa.gov!lll-winken.llnl.gov!fnnews.fnal.gov!nntp-server.caltech.edu!NewsWatcher!user From: grovesa@starbase1.caltech.edu (Andrew K. Groves) Newsgroups: bionet.molbio.ageing,sci.life-extension Subject: Re: solid data that says cancer caused by ageing process (Forwarded article) Date: 6 Sep 1995 17:43:12 GMT Organization: California Institute of Technology Lines: 34 Message-ID: References: NNTP-Posting-Host: mac168.bio.caltech.edu Xref: biosci bionet.molbio.ageing:1942 sci.life-extension:7053 In article , steve@chambers.ak.planet.co.nz (Steve Chambers) wrote: > Sure, why not? Just because an aging process is most observable late > in life doesn't mean that it hasn't been going on throughout. Adults may > not have neuroblasts and other early development cell types but that's > not important - it's the somatic mutations (which can happen at any age) > that are the "aging" process of interest here. > > Besides, we're quite happy to conceptualize Werner's and other progerias > as premature aging. Why not other age-linked phenomenon that occur in > childhood? > I can't really carry on the debate, as I don't know enough about the molecular lesions that have been implicated in embryonic and childhood cancers.I guess that if the mutation was caused by a mutation in the *parent's* germ cells, then one could quite easily claim it to be age-related!!! > > PS Andy - We're talking about aging of whole organisms here ;-) > Good to hear from you again. > Organisms? What are they? Can you grow them in a dish????? :) -- Andy Groves Division of Biology, 216-76 California Institute of Technology From owner-ageing@net.bio.net Wed Sep 06 23:00:00 1995 Path: biosci!demo.ucl.ac.be!wunsch From: wunsch@demo.ucl.ac.be (WUNSCH Guillaume) Newsgroups: bionet.molbio.ageing Subject: New book on Longevity Research Date: 7 Sep 1995 06:18:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 75 Sender: daemon@net.bio.net Distribution: world Message-ID: <9509071308.AA12927@sci1.sri.ucl.ac.be> NNTP-Posting-Host: net.bio.net Dear Colleagues, After our recent 3 presentations at the III European Congress of Gerontology in Amsterdam we have received an invitation from the Karger Publisher (from Dr.Thomas J.Karger personally) to write a book about our new results in addition to our previous book "The Biology of Life Span" published in 1991 by Harwood Academic Publishers. We can easily write the whole new book ourselves but it seems to us very interesting to try to create an international team of book coauthors since it will promote greatly scientific discussions and debates and scientific collaboration within such a team. We would like to invite at least one scientist from Europe, USA and Japan to join us in our new book project and to write a book that will be a result of scientific discussions between scientists of these different cultures. Sincerely yours, Dr.Leonid A.Gavrilov, Ph.D. Member of the Council of Russian Gerontological Society Principal Research Scientist, Moscow State University Dr.Natalia S.Gavrilova, Ph.D. Senior Research Scientist Instutute of System Analysis, Russian Academy of Sciences P.S.: Our detailed schedule is printed below for your information. ********************************************************************* 1. Belgium. September 5-13, 15-20. Contact person: Professor Guillaume Wunsch. Phone: (32 10) 47 29 51 Fax: (32 10) 47 29 52 Working Address: Dr.Leonid A.Gavrilov, Ph.D. c/o Prof.G.Wunsch Institut de demographie place Montesquieu, 1/17 B-1348 Louvain-la-Neuve Belgium Home Address: Dr.Leonid A.Gavrilov, Ph.D. c/o Mrs.Cecile Goossens Clos des Molons, 1 B-1348 Louvain-la-Neuve Belgium Home phone: (32 10) 45 07 67 2. United Kingdom. September 13-14 and perhaps later. Contact person: Dr. Michael Murphy. Phone: 0171-405 7686 Fax: 0171-955 6833 Address: Dr.Leonid A.Gavrilov,Ph.D. c/o Dr.M.Murphy Population Studies London School of Economics Houghton Street London WC2A 2AE UK 3. France. September 21-22 and perhaps later. Contact person: Dr.Michel Allard. Phone: 33(1) 44 96 10 95 Fax: 33(1) 44 96 11 99 Address: Dr. Leonid A.Gavrilov,Ph.D. c/o Dr.Michel Allard IPSEN Foundation 24, rue Erlanger 75781 PARIS cedex 16 France P.S.: This list might be extended in future. From owner-ageing@net.bio.net Thu Sep 07 23:00:00 1995 Newsgroups: bionet.molbio.ageing Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!in2.uu.net!nih-csl!postman From: Robert hewitt Subject: apoptosis and trypsinization Content-Type: text/plain; charset=us-ascii Message-ID: <1995Sep8.005448.14045@alw.nih.gov> Sender: postman@alw.nih.gov (AMDS Postmaster) Nntp-Posting-Host: 128.231.221.68 Content-Transfer-Encoding: 7bit Organization: NCI, NIH Mime-Version: 1.0 Date: Fri, 8 Sep 1995 00:54:48 GMT X-Mailer: Mozilla 1.1N (Macintosh; I; PPC) X-Url: news: bionet.molbio.ageing Lines: 31 Dear Internetter, I have been trying to estimate the level of apoptosis in the SW480 colon cancer cell line by propidium iodide staining and flow cytometry. The result I get is that 30% of cells are apoptotic, which seems awfully high! I wonder whether I may be inducing apotosis when preparing the single cell suspension. The procedure I use is to trypsinize, resuspend in DMEM + 10% FBS, spin at 700 rpm for 5 mins in a Sorvall benchtop, resuspend in PBS without calcium or magnesium, count, then spin again, then resuspend in an appropriate volume of PBS, before fixation. All this is completed in between 40 and 60 minutes. The whole procedure is done at room temperature to avoid "cold shock" induction of apoptosis. Is it likely that I could be inducing apoptosis by the above procedure? If so, then would there be evidence of apotosis given the fact that cells are fixed within 60 minutes of trypsinization? Any comments would be gratefully received. Thanks in advance, Robert Hewitt. From owner-ageing@net.bio.net Fri Sep 08 23:00:00 1995 Path: biosci!agate!newsxfer.itd.umich.edu!newsfeed.internetmci.com!EU.net!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: mikemldvn@aol.com (MikeMldvn) Newsgroups: bionet.molbio.ageing Subject: Talking to the hard of hearing Date: 7 Sep 1995 03:23:53 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 37 Sender: root@newsbf02.news.aol.com Message-ID: <42m6m9$gnd@newsbf02.news.aol.com> Reply-To: mikemldvn@aol.com (MikeMldvn) Talking to the hard of hearing About a year or so ago I posted in several places online a list of *tips for talking to the hard of hearing*. I plan to repeat the posting online and to also e-mail the list to news services and other media as a *public service* informational item. Before I do so, I invite additional *tips* from hearing experts, teachers, and other knowledgeable persons through this general posting which is being placed in several AOL forums and Internet newsgroups. Please e-mail your input to (AOL) mikemldvn or (Internet) mikemldvn@aol.com The *tips* that I have to date include the following: 1. Face the hard of hearing person directly, and on the same level with him/her, whenever possible. 2. If you are eating, chewing, smoking, etc., while talking, your speech will be more difficult to understand. 3. Reduce background noises when carrying on conversations -- turn off the radio or TV. 4. Keep your hands away from your face while talking. 5. If a person has difficulty understanding something, find a different way of saying the same thing, rather than repeating the original words over and over. 6. Recognize that hard of hearing people hear and undertand less well when they are tired or ill. 7. Never talk from another room. Be sure to get the person's attention before you start speaking to him/her. 8. Speak in a normal fashion without shouting. See that the light is not shining in the eyes of the hard of hearing person. Mike Moldeven From owner-ageing@net.bio.net Sat Sep 09 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!in2.uu.net!news.compuserve.com!news.production.compuserve.com!news From: Patrick R. Jones <102570.2734@CompuServe.COM> Newsgroups: bionet.molbio.ageing,bionet.molbio.bio-matrix,bionet.molbio.embldatabank,bionet.molbio.evolution,bionet.molbio.gdb Subject: Need Help Date: 10 Sep 1995 20:08:40 GMT Organization: Personal Lines: 19 Message-ID: <42vgk8$og2$8@mhafn.production.compuserve.com> Xref: biosci bionet.molbio.ageing:1947 bionet.molbio.bio-matrix:632 bionet.molbio.embldatabank:540 bionet.molbio.evolution:3432 bionet.molbio.gdb:360 I am a undergraduate student in biology. Some day I hope to go to graduate school in biochemistry. My grades are good enough but I am afraid that this may not be enough to get in. I am looking for something to set myself apart from the other students. At this point I am at a loss on what to do. I have no real resources to work on projects in the areas that interest me. Does anybody have any ideas? I do have one useful skill. I am a professional computer programmer. I don’t mean someone who has taken a FORTRAN class and is working as a work-study student, but a 10 year application programming veteran in the computing industry. If I could use this skill great if not, I don’t care. I need something to further my career; something good. Please help!!! I know that somebody out there knows of something I could do. Thank you for your time!!! Patrick R. Jones -- <> From owner-ageing@net.bio.net Sun Sep 10 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news From: ray and lisa <71623.3126@CompuServe.COM> Newsgroups: bionet.molbio.ageing Subject: NEW ANTI-AGING DRUG!!! Date: 11 Sep 1995 00:18:17 GMT Organization: n/a Lines: 3 Message-ID: <42vv89$t7s$1@mhade.production.compuserve.com> heard about new anti-aging drug out in Austria. would like to find out more about it & how to obtain it. info about other proven drugs would be helpful too. From owner-ageing@net.bio.net Mon Sep 11 23:00:00 1995 Newsgroups: bionet.molbio.ageing,bionet.molbio.bio-matrix,bionet.molbio.embldatabank,bionet.molbio.evolution,bionet.molbio.gdb Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news.cac.psu.edu!news.math.psu.edu!psuvax1!gvls1!newsfeed.pitt.edu!godot.cc.duq.edu!news.duke.edu!news-server.ncren.net!hearst.acc.Virginia.EDU!murdoch!darwin.clas.Virginia.EDU!mgk From: mgk@darwin.clas.Virginia.EDU (Mahlon G. Kelly) Subject: Re: Need Help X-Nntp-Posting-Host: darwin.clas.virginia.edu Message-ID: Sender: usenet@murdoch.acc.Virginia.EDU Organization: uva References: <42vgk8$og2$8@mhafn.production.compuserve.com> Date: Tue, 12 Sep 1995 06:23:38 GMT Lines: 33 Xref: biosci bionet.molbio.ageing:1949 bionet.molbio.bio-matrix:633 bionet.molbio.embldatabank:541 bionet.molbio.evolution:3445 bionet.molbio.gdb:361 Patrick R. Jones writes: > I am a undergraduate student in biology. Some day I hope to go to > graduate school in biochemistry. My grades are good enough but I > am afraid that this may not be enough to get in. I am looking for > something to set myself apart from the other students. At this > point I am at a loss on what to do. I have no real resources to > work on projects in the areas that interest me. Does anybody have > any ideas? I do have one useful skill. I am a professional > computer programmer. I don’t mean someone who has taken a FORTRAN > class and is working as a work-study student, but a 10 year > application programming veteran in the computing industry. If I > could use this skill great if not, I don’t care. I need something > to further my career; something good. Please help!!! I know that > somebody out there knows of something I could do. > > Thank you for your time!!! > Patrick R. Jones > > -- > <> Certainly there must be some investigator, either in your university or at a nearby medical center, who would be only too happy to use your skills in a for-credit research project. That would certainly look good on your application. Another hint: try to get to know your faculty members well enough that when it comes time to get letters of recommendation they can write something fairly personal, not just a form letter that says they had you in a class. -- Associate Professor (Emeritus) University of Virginia mgk@darwin.clas.virginia.edu From owner-ageing@net.bio.net Wed Sep 13 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!news.uoregon.edu!vixen.cso.uiuc.edu!uchinews!apollo.it.luc.edu!usenet From: nonn et al Newsgroups: bionet.molbio.ageing,bionet.molbio.bio-matrix,bionet.molbio.embldatabank,bionet.molbio.evolution,bionet.molbio.gdb Subject: Re: Need Help Date: 14 Sep 1995 02:21:56 GMT Organization: Loyola univ Chicago Lines: 2 Message-ID: <4383k4$dcj@apollo.it.luc.edu> References: <42vgk8$og2$8@mhafn.production.compuserve.com> NNTP-Posting-Host: 147.126.163.163 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2b2 (Windows; I; 16bit) To: 102570.2734@CompuServe.COM Xref: biosci bionet.molbio.ageing:1950 bionet.molbio.bio-matrix:634 bionet.molbio.embldatabank:542 bionet.molbio.evolution:3461 bionet.molbio.gdb:363 Grades are not enough, publish like a fiend and suck up to the big guns! From owner-ageing@net.bio.net Thu Sep 14 23:00:00 1995 Path: biosci!OZEMAIL.COM.AU!pjhynes From: pjhynes@OZEMAIL.COM.AU (Peter Hynes) Newsgroups: bionet.molbio.ageing Subject: Subscribe Date: 15 Sep 1995 16:11:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <199509152308.JAA14090@oznet02.ozemail.com.au> NNTP-Posting-Host: net.bio.net This is a test .. to forget it would be best. From owner-ageing@net.bio.net Fri Sep 15 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.sprintlink.net!in1.uu.net!news1.digital.com!ames!lll-winken.llnl.gov!fnnews.fnal.gov!nntp-server.caltech.edu!NewsWatcher!user From: cherbert@cco.caltech.edu (Curtis Herbert) Newsgroups: bionet.molbio.ageing,bionet.molbio.bio-matrix,bionet.molbio.embldatabank,bionet.molbio.evolution,bionet.molbio.gdb Subject: Re: Need Help Date: Fri, 15 Sep 1995 18:48:47 +0900 Organization: Caltech Lines: 24 Message-ID: References: <42vgk8$og2$8@mhafn.production.compuserve.com> NNTP-Posting-Host: 131.215.249.3 Xref: biosci bionet.molbio.ageing:1952 bionet.molbio.bio-matrix:635 bionet.molbio.embldatabank:543 bionet.molbio.evolution:3480 bionet.molbio.gdb:365 In article <42vgk8$og2$8@mhafn.production.compuserve.com>, Patrick R. Jones <102570.2734@CompuServe.COM> wrote: > I am a undergraduate student in biology. Some day I hope to go to > graduate school in biochemistry. My grades are good enough but I > am afraid that this may not be enough to get in. I am looking for > something to set myself apart from the other students. snip > > -- > <> Who's law? I also urge you to try to do some undergraduate research. Make up a little resume and hand-deliver it to some likely faculty members. Ask them who might be interested in you. Also try to keep an eye open for ways to apply your background; you have a valuable skill. You might mention it to some of the faculty types. Also, a better posting for this sort of thing might be: alt.grad-student.tenured Curtis H. From owner-ageing@net.bio.net Sat Sep 16 23:00:00 1995 Newsgroups: bionet.molbio.ageing,bionet.molbio.bio-matrix,bionet.molbio.embldatabank,bionet.molbio.evolution,bionet.molbio.gdb Path: biosci!agate!howland.reston.ans.net!torn!utnut!utzoo!mes From: mes@zoo.toronto.edu (Mark Siddall) Subject: Re: Need Help Message-ID: Date: Sun, 17 Sep 1995 05:37:33 GMT References: <42vgk8$og2$8@mhafn.production.compuserve.com> <4383k4$dcj@apollo.it.luc.edu> Organization: U of Toronto Zoology Lines: 17 Xref: biosci bionet.molbio.ageing:1953 bionet.molbio.bio-matrix:636 bionet.molbio.embldatabank:544 bionet.molbio.evolution:3490 bionet.molbio.gdb:366 In article <4383k4$dcj@apollo.it.luc.edu> nonn et al writes: >Grades are not enough, publish like a fiend and suck up to the big guns! > Good point, though I would add that any sucking up (or even lack thereof) is going to make people think "Hmmm. what camp are they in...?" and you will inevitably be taken on those grounds rather than for what you might have to say. It's a shame, but as a young phylogeneticist, I assure you it's true. If there's one thing that the majority does NOT respect out there... it's a firm conviction. So don't have one... you'll go further. -- Mark E. Siddall "I don't mind a parasite... mes@vims.edu I object to a cut-rate one" Virginia Inst. Marine Sci. - Rick Gloucester Point, VA, 23062 From owner-ageing@net.bio.net Sat Sep 16 23:00:00 1995 Path: biosci!agate!library.ucla.edu!info.ucla.edu!newsfeed.internetmci.com!news.sprintlink.net!parsifal.nando.net!usenet From: newfound@vistech.com Newsgroups: bionet.agroforestry,bionet.biology.tropical,bionet.jobs,bionet.jobs.wanted,bionet.molbio.ageing,bionet.molbio.evolution Subject: Simply Success-->Eat SBG Algae-->Gain Health, Energy, Time, and Financial Freedom... Date: Mon, 18 Sep 1995 01:47:03 GMT Organization: News & Observer Public Access Lines: 117 Message-ID: <43i8l5$b23@parsifal.nando.net> NNTP-Posting-Host: grail1109.nando.net X-Newsreader: Forte Free Agent 1.0.82 Xref: biosci bionet.agroforestry:2005 bionet.biology.tropical:1249 bionet.jobs.wanted:2597 bionet.molbio.ageing:1954 bionet.molbio.evolution:3493 You just took the first step to a better life. First of all, this is a legitimate Multi-Level Marketing (MLM) business that has been around for 13 years with more than 200,000 distributors. (Note: Less than 5% of MLM’s survive their second year). We at Newfound Energy can show you how to gain: Health/weight loss Physical energy Mental clarity and alertness A network of distributors that --we build for you!!-- Financial freedom ($500- $1500/ month in 6-12 months) Satisfaction of charity contribution (see 10% solution below) The above list of benefits will change your life -- It sure changed mine. Check out our home page, this business will stand the scrutiny of investigation. Newfound Energy with Cell Tech http://www.vistech.net/users/newfound We are independent distributors of Super Blue Green Algae (c) (SBGA) produced by Cell Tech (c). SBGA is the most pure form of Algae on the market. Cell Tech (c) offers a complete line of products (based on SBGA). It is harvested from a lake in the pristine Cascade Mountains in Oregon. All nutrients are quickly locked into the tablets and capsules by a patented process. As a result, our bodies can easily absorb this source of protein, enzymes, and vitamins. As a distributor, you can consume this algae at wholesale prices (30 % off) and enjoy the benefit of this super-food. Many distributors do not resell the product, they simply consume the product and tell their friends and family about the results. As they see the improvement in your health, energy, and financial freedom they will be curious and check it out for themselves. 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Unique product There is only one Super Blue Green Algae because no other environment on earth duplicates the wild and fertile ecology of Klamath Lake. By comparison, commercial freshwater Spirulina and Chlorella must be grown in man-made ponds, under artificially controlled conditions. In these ponds the algae receives a smaller quantity of minerals and, more important, a far narrower range of nonorganic minerals. 8. Health, energy and time to do more of what you want! Consider all of these factors when comparing this MLM with others. Finally, you will have the sense of satisfaction that comes with contributing to charity. Cell Tech (c) committed in 1992 to help feed the masses and introduced the 10% Solution program. From then on, 10% of the yearly harvest will go “to people who have the greatest need and the fewest resources.” The following projects are now underway: Nicaragua Cambodia Chernobyl Los Angeles (Cultivating Self-Worth in an Urban War Zone) American Indian Family Healing Center --------------------------------------------------------------------------------------- For more information, please contact me: Allen A. Harper email: newfound@vistech.net At least check out our home page, this business will stand the scrutiny of investigation. Don’t waste time. http://www.vistech.net/users/newfound or call Newfound Energy at 1-800-519-0013 PS: “wise men and women simply investigate what fools pass by...” From owner-ageing@net.bio.net Tue Sep 19 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!parsifal.nando.net!usenet From: newfound@vistech.net Newsgroups: bionet.agroforestry,bionet.biology.tropical,bionet.jobs,bionet.jobs.wanted,bionet.molbio.ageing,bionet.molbio.evolution Subject: Correction--> New Found Energy's email address is newfound@vistech.net (not newfound@vistech.com) Date: Wed, 20 Sep 1995 14:37:42 GMT Organization: News & Observer Public Access Lines: 9 Message-ID: <43ouie$ct1@parsifal.nando.net> NNTP-Posting-Host: grail1210.nando.net X-Newsreader: Forte Free Agent 1.0.82 Xref: biosci bionet.agroforestry:2010 bionet.biology.tropical:1254 bionet.jobs.wanted:2636 bionet.molbio.ageing:1955 bionet.molbio.evolution:3508 Sorry, On several previous postings our email address was incorrect. We have no connection with vistech.com. See our home page for details of our products: http://www.vistech.net/users/newfound ^ | From owner-ageing@net.bio.net Tue Sep 19 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!news.crd.ge.com!rebecca!gk1702 From: gk1702@csc.albany.edu (KRISHNA GOPALA S) Newsgroups: bionet.molbio.ageing Subject: Electron Microscopy Study! Date: 20 Sep 1995 18:09:56 GMT Organization: The University at Albany Lines: 21 Distribution: na Message-ID: <43pldk$ovm@rebecca.albany.edu> NNTP-Posting-Host: lilith.albany.edu ******************************************************** Do You Have Work On Transmission, Scanning, Cr- Electron Microscopes, Elemental Analysis and Image Analysis? ********************************************************* I am a Certified Electron Microscopist from V.A.Hospital, Albany, NY and I have two years experience on specimen preparation, processing for Transmission, Scanning, Cryo- Electron Microscopes, Ultramicrotomy, Photographic techniques. If you send your specimen samples, I can process them and take very nice pictures under Transmission, Scanning Electron Microscopes and also perform elemental analysis under SEM and mail them to you. I charge very less money per sample comparative to other people. I charge $10 per specimen. I can process any plant, animal specimens for Electron Microscpy study. Please send $10 cash or check and specimen sample to Mr.Krishna, 248 Shakerrun Apts, Albany, NY-12205. ************************************************************* From owner-ageing@net.bio.net Wed Sep 20 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!uunet!in1.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: spilchard@aol.com (S pilchard) Newsgroups: bionet.molbio.ageing Subject: Questions About Baldness Date: 20 Sep 1995 21:44:32 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 7 Sender: root@newsbf02.news.aol.com Message-ID: <43qg20$sk7@newsbf02.news.aol.com> Reply-To: spilchard@aol.com (S pilchard) NNTP-Posting-Host: newsbf02.mail.aol.com I'm looking for reliable information concerning baldness, thinning hair, and male pattern-baldness (I don't know any of the distinctions) and their purported causes. I'm also interested in any information about preventing, arresting, or reversing receding hairlines, etc. Perhaps, you have some information or you can refer me to some sources. Thanks From owner-ageing@net.bio.net Wed Sep 20 23:00:00 1995 Path: biosci!LEX.LCCC.EDU!rcb1 From: rcb1@LEX.LCCC.EDU (Ron Blue) Newsgroups: bionet.molbio.ageing Subject: neuromodel Date: 21 Sep 1995 06:11:22 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 69 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <43qg20$sk7@newsbf02.news.aol.com> NNTP-Posting-Host: net.bio.net The following is available by FTP: Note: this is Unix system which is case sensitive. ftp.tmn.com login: anonymous password: (your email address) cd Chaos-Complexity ls get BlueBlue-OpponentProcessing.txt bye Abstract: The correlational opponent-processing theory is a neuro homeostasis integration psychological immune theory that would connect phenomena such as sensation, perception, movement, habituation, memory, representations, learning, cognition, personality, psychopathology, paradoxical integration, emotion, and evolution of the mind under a unified theory. Perception/learning/cognition may be viewed as an effort to assimilate and accommodate all experience into neuro-energy-efficient eigenfunction equivalence or quasi-holographic correlational opponent- processing recordings. Stimuli causes brain wave modulations which interact with carrier or reference wavelets. This interaction creates a quasi-holographic stimulus wavelet. The opponent-process creates an opposing quasi- holographic memory wavelet. Through this process the correlations or associations of experience are encoded to memory. Every wavelet, regardless of source or type, triggers an opposing wavelet. The function of the opposing wavelet or feedback is to diminish the intensity of neural processing. A wavelet potential is stored or hard wired as long-term potentiation opponent-processes in nerve cells and the interconnections between nerve cells. The wavelets are quasi- holographic and allow recovery of information due to the interaction of reference carrier wavelets and stimuli, thought, motor movement, and emotional arousal. Outline: Discussion Neuro Net Quasi-holographic wavelets Habituation/immunization Memory Representations, copies or models Learning/Cognition Personality Sensations and Perceptions Movement Emotion Evolution Tools Implications Conclusion and applications from COP theory Discorrelation Education Biophysical Intelligence Defense Mechanisms Brain damage Creativity Brain Tape Computer Model Conclusion Bibliography Acknowledgments From owner-ageing@net.bio.net Wed Sep 20 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!news.crd.ge.com!rebecca!gk1702 From: gk1702@csc.albany.edu (KRISHNA GOPALA S) Newsgroups: bionet.molbio.ageing Subject: Electron Microscopy Study! Date: 21 Sep 1995 16:10:05 GMT Organization: The University at Albany Lines: 66 Distribution: na Message-ID: <43s2ot$fu@rebecca.albany.edu> NNTP-Posting-Host: eve.albany.edu ******************************************************** Do You Have Work On Transmission, Scanning, Cr- Electron Microscopes, Elemental Analysis and Image Analysis? ********************************************************* I am a Certified Electron Microscopist from V.A.Hospital, Albany, NY and I have two years experience on specimen preparation, processing for Transmission, Scanning, Cryo- Electron Microscopes, Ultramicrotomy, Photographic techniques. If you send your specimen samples, I can process them and take very nice pictures under Transmission, Scanning Electron Microscopes and also perform elemental analysis under SEM and mail them to you. I charge very less money per sample comparative to other people. I charge $50 per sample. I can process any plant, animal specimens for Electron Microscpy study. Please send $50 cash or check and specimen sample to Mr.Krishna, 248 Shakerrun Apts, Albany, NY-12205. Please E-mail your questions to: gk1702@ix.netcom.com Approximate time for specimen preparation, processing for TEM and SEM: Specimen processing time for TEM 24 Hours & SEM: Ultramicrotomy, thick, thin section slides preparation, positive, negative staining techniques, observation under Transmission electron microscope depending upon microscope time and photography techniques 1 to 2 days Specimen preparation for SEM i.e. sputter coating or sputtering or carbon coating, observing under SEM and taking pictures 1 to 2 days I need to pay for chemicals, electron microscopy time, for purchasing film for both TEM and SEM etc that is why I am charging $50 per specimen. I apologize I mentioned $10 per sample in my last posting and it is not feasible for me. P.S.: This is not scam or some thing nonsense. I completed B.S. in Biology and Chemistry, M.S. in Biology, Master of Philosophy in Biology, one year intensive training on Electron Microscopy at The School Of Electron Microscopy, Veterans Hospital, Albany, NY and I am a certified computerprogrammer. I have strong ambition to utilise my Electron Microscopy skills in the field of Biology and Medicine. This way, I can fulfil my ambition to become a diagnostic Electron Microscopist and inturn I will be useful to The School Of Electron Microscopy, V.A.Hospital, Albany, NY. ************************************************************* From owner-ageing@net.bio.net Wed Sep 20 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!news.sprintlink.net!in2.uu.net!EU.net!sun4nl!news.euro.net!usenet From: Luc Sala Newsgroups: bionet.molbio.ageing Subject: WANTED addresses of popular health magazines Date: 21 Sep 1995 20:21:38 GMT Organization: Sala Communications Lines: 15 Message-ID: <43shgi$n42@news.euro.net> NNTP-Posting-Host: p418.asp.euronet.nl Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2b6 (Windows; I; 16bit) Hi, I would like to receive addresses plus phonenumbers of magazines that publish articles concerning health and/or life extension, and which are aimed at the general public. Please don't reply to this email, but send mail to: ferry@info.uba.uva.nl Many thanks in advance, Ferry From owner-ageing@net.bio.net Thu Sep 21 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!howland.reston.ans.net!news.sprintlink.net!in2.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: aprilworks@aol.com (AprilWorks) Newsgroups: bionet.molbio.ageing Subject: Retirement Living Date: 22 Sep 1995 10:22:34 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 11 Sender: root@newsbf02.news.aol.com Message-ID: <43ugra$4ft@newsbf02.news.aol.com> Reply-To: aprilworks@aol.com (AprilWorks) NNTP-Posting-Host: newsbf02.mail.aol.com Can anyone help me locate guides to continuing care retirement communities. I am looking for any information that would help me find an appropriate environment for my mother and her husband, who are in their 80's. This may not be the appropriate newsgroup for answering this question, so would also appreciate any guidance in finding the newsgroup which would be most helpful to me. I've looked and looked on the internet and can't seem to find anything which addresses my questions. TIA April AprilWorks@aol.com From owner-ageing@net.bio.net Thu Sep 21 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news.nic.surfnet.nl!highway.LeidenUniv.nl!usenet From: Angelo Schouten Newsgroups: bionet.molbio.ageing Subject: This bionet newsgroup is alienated from its origin Date: 21 Sep 1995 13:48:52 GMT Organization: Leiden University, The Netherlands Lines: 15 Message-ID: <43rqg4$hsd@highway.LeidenUniv.nl> NNTP-Posting-Host: rulc12.leidenuniv.nl Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) I always considered this bionet newsgroup to be a meeting place for experts on ageing. Why do I see so many commercial ads and "nag nags". Is this the inherent policy or simply a plain weakness? No wonder that those seriously devoted to ageing seek comfort elsewhere. If gangrene starts to spread, it is better to amputate as quickly as possible. Not very funny unfortunately. Yours truly, Angelo Schouten (Physicist/Chemist) From owner-ageing@net.bio.net Thu Sep 21 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.sprintlink.net!simtel!harbinger.cc.monash.edu.au!sol.ccs.deakin.edu.au!pc-rbp161.sci.deakin.edu.au!drierac From: drierac@deakin.edu.au (Chris Driver) Newsgroups: bionet.molbio.ageing Subject: DHEA Date: Fri, 22 Sep 1995 18:34:25 Organization: Deakin University Lines: 12 Message-ID: NNTP-Posting-Host: pc-rbp161.sci.deakin.edu.au X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] To all readers and lurkers.. Will somemake some sort of comments about DHEA. Esepcially if it works and why it does (if it does) Chris Dirver Chris Driver, Ph D School of Biology and Chemistry, Rusden Campus Deakin University 662 Blackburn Rd Clayton, VIC, 3168 AUSTRALIA From owner-ageing@net.bio.net Thu Sep 21 23:00:00 1995 Path: biosci!biosci!not-for-mail From: Jorge Braz Newsgroups: bionet.molbio.ageing Subject: Oxidative stress as a cause for ageing Date: 21 Sep 1995 22:19:15 -0700 Organization: Faculdade de Ciencias da Universidade de Lisboa Lines: 4 Sender: biohelp@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I am looking for any information about the reactions of oxygen radicals (o2.-, HO., O1, H2O2) with the DNA bases especially the rate constants and the products percentages of this reactions. Jorge Braz From owner-ageing@net.bio.net Thu Sep 21 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!news.sprintlink.net!news.onramp.net!news.tcst.com!dildog.lgc.com!news.sesqui.net!uuneo.neosoft.com!sam-slip-i2.NeoSoft.COM!pproctor From: pproctor@sam.neosoft.com (Peter H. Proctor) Newsgroups: bionet.molbio.ageing Subject: Re: Questions About Baldness-- FAQ Date: Thu, 21 Sep 1995 11:03:20 UNDEFINED Organization: NeoSoft Internet Services +1 713 968 5800 Lines: 235 Message-ID: References: <43qg20$sk7@newsbf02.news.aol.com> NNTP-Posting-Host: sam-slip-i2.neosoft.com Keywords: Alopecia, balding, hairloss X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #1] In article <43qg20$sk7@newsbf02.news.aol.com> spilchard@aol.com (S pilchard) writes: >From: spilchard@aol.com (S pilchard) >Subject: Questions About Baldness >Date: 20 Sep 1995 21:44:32 -0400 >I'm looking for reliable information concerning baldness, thinning hair, >and male pattern-baldness (I don't know any of the distinctions) and their >purported causes. I'm also interested in any information about >preventing, arresting, or reversing receding hairlines, etc. Perhaps, you >have some information or you can refer me to some sources. FAQ: Hair-loss and Balding Part 1: Pathophysiology and some treatment agents Pattern Balding: Incidence in males roughly corresponds to age (i.e.,at age 35, about 35% of males have it ). Incidence in women half to third this, at least before menopause. *Inheritance complicated. Possibly: "autosomal dominant with mixed penetrance". Translation: you can inherit from mom or dad and it expresses itself variably. *What you inherit is susceptibility to male sex hormones. multiple factors, including increased uptake, conversion to more active forms, receptor sites may be involved. *Four major androgens: Testosterone, DHT, DHEA, and androstenedione. DHT most potent-- but, DHEA from adrenals important in women and possibly men. Likewise, androstene dione in men. Other factors in balding: immunological, damage to blood vessel lining. _Emerging Model for Pattern Balding_ (after Kligman, others ) Hormones do something to hair follicle which causes it to be read as "foreign body" by immune system, which then mounts an attack. Main damage in balding is probably immunologically-mediated. Damage to lining of blood vessels, which produces hair growth-stimulatory factors, makes this worse. A) Evidence for hormonal factors: Castration, lack of DHT-receptors/enzymes (testicular feminization) , feminine status block progression. However, women and castrated males have other sources of androgens and can still experience pattern loss. Even complete castration ( as in male to female sex change operations ) does not reverse balding much. Immunological factors * Microscopically, Balding looks like organ rejection. Cells of your immune system clustor around the follicle base. BTW, Immune system cells normally cluster around the hair follicle. They may have a role in the normal hair cyle. * Organ rejection drugs ( e.g., cyclosporin ) reverse balding a whole lot better than antiandrogens. This gives a rough indication of the relative importance of hormonal verses immunological factors in maintaining the balding state. * Antibodies to hair follicles present in blood. B) Blood Vessel Lining evidence *Minoxidil, other agents may imitate hair growth factors ( nitric oxide radical, etc. ) produced by vessel lining. In diseases involving damage to vessel lining (e.g.,atherosclerosis) production of these is decreased. Such diseases are associated epidemiologically with severe balding. Also, decreases in circulation reported in balding scalp may reflect local damage to vessel lining II) Treatment of Pattern Loss Aside: Forget looking in the medical literature for new agents. Because of the commercial potential, everyone (including me) goes after patents. Most drug companies want to keep things secret as long as possible and so often don't publish on a new drug until it's just about ready for commercial release. BTW, when developing any drug, the first place a PhD pharmacologist ( the guys who really develop drugs ) looks is in the patent literature. Most physicians and nonpharmacologist biomedical researchers do not know about about looking at patents, so you will rarely see them quoted. Even if a researcher is just interested in basic mechanisms, this is a bad mistake. For example, several patents from multiple sources indicate that superoxide dismutases stimulate hair growth. Further, another patent from the Procter and Gamble Company involves using an inhibitor of SODases ( DDTC ) to inhibit hair growth. The implication is that superoxide radical ( an important messenger in many other systems) is also a messenger modulating hair growth. There is not a hint of this in the "open" biomedical literature. Besides, at last count, over forty US and several hundred foreign patents are issued in this area. Probably most work at least some--few if any have been published. Treatment Agents A) Antiandrogens: E.g.: Proscar, Cyoctal, spironolactone. Poorly-effective alone. Mainly useful as adjuvants to other therapy where they 1) make it work better 2) Help prevent tolerance. Every few years, a new antiandrogen will be presented as the ultimate "solution for balding". This has yet to work out. E.g., clinical trials with cyoctal, arguably the most potent topical antiandrogen, were terminated because of lack of effectiveness. We'll see about proscar ( the weakest one ). Early reports suggest it works about as well as topical spironolactone or cyoctal ( e.g., about half of patients show a 10% or greater number of hairs on their heads after a year on treatment ). Because Proscar is already FDA-approved for prostate enlargement, Merck will have an easier time getting FDA opproval for it. BTW, I have prime patents in this area ( for growth stimulators plus antiandrogens ). In fact, because of the publication of our patents, the combination of a hair growth stimulator plus and antiandrogen is now " obvious " and thus unpatentable. I sure wish antiandrogens worked better. Possible explaination: Male hormones only initiate balding. Further, whatever hormones do seems to be mostly irreversible. The main damage to the hair follicle seems to be done by other factors, especially immunological. B) Direct Hair Growth Stimulators There are two main kinds: 1) Agents like minoxidil. These mimic the transmitter substance nitric oxide, which tells hair to grow and not fall out. 2) SODases and related agents. These destroy the tramsmitter substance superoxide, which tells hair to stop growing and fall out. Examples Nitroxides: Minoxidil, nicorandil, NANO, etc. Some of these are blood vessel dialating hair-growth stimulators. OTOH, most dialating agents don't stimulate hair growth, nor do all these agents dialate blood vessels. So, vessel dilatation is not _directly_ related to hair growth stimulation. Possible explaination: Nitrovasodialators mimic natural substance mediating _both_ dialation and hair growth. Best candidate: nitric oxide ( aka, EDRF ), a ubiquitous transmitter which has identical effects to minoxidil on blood vessels. Superoxide Dismutase Mimetics. These include Iamin, " trichomin" (Procyte corporation), Copper-Binding Peptide (Procter and Gamble), TEMPOL, others (In the late 70's, we found that SODase prevents stress loss in experimental animals ). Another copper-binding peptide SODase hair-growth stimulator made from soy protein was just patented. Other radical scavengers also effective ( e.g., various US and foreign patents-- including some to us.) BTW, the US patent office just awarded me the prime patent for SODases in hair loss. Further, this technology is so mature that the Procter and Gamble corporation has a US patent on a SODase inhibitor ( Diethyldithiocarbamate ) to PREVENT hair growth. SODases destroy superoxide free radical: Superoxide reacts with nitric oxide ( the putative "natural" minoxidil ) to produce other toxic free radical products. OTOH, in many systems, superoxide is a messenger substance in its own right. In such systems, it ofter opposes the action of nitric oxide. So, SODases may work by increasing nitric oxide levels or more directly. Alternately, such agents may interfere with the immunological component in hair growth. Active oxygen species are the most important mediators of cell-mediated immunity. BTW, a mild infiltrate of immune cells develops around the normal follicle as the hair cycle progresses. This may be the source of the superoxide that tells hair to stop growing. Significantly, nitric oxide and superoxide have opposing "yin-yang" effects on a number of bodily systems. Examples include blood-vessel dialatation ( nitric oxide dialates, superoxide constricts ), blood clotting, etc. These paired agents may be the most ubiquitous transmitter substances around. You can explain a lot by assuming that nitric oxide is the transmitter that initiates and maintains hair growth, while superoxide both inhibits hair growth and causes hair to transition from the growth phase to the loss phase. __________________________________________________________ I am an MD,PHD pharmacologist who practices dermatology and does hair-loss research. Repost, but please leave the credit. BTW: Please address general questions to the newsgroup so that I don't have to keep answering the same ones over and over. Peter H. Proctor, MD,PhD Suite 1616, 4126 SW Freeway Houston, TX 77027 (713) 960-1616 pproctor@sam.neosoft.com Shameless Plug: Send your surface mail address and we'll tell you about our program for treating hair loss. Sorry, our stuff is not in electronic form yet. c 1995 Peter H. Proctor From owner-ageing@net.bio.net Thu Sep 21 23:00:00 1995 Path: biosci!LEX.LCCC.EDU!rcb1 From: rcb1@LEX.LCCC.EDU (Ron Blue) Newsgroups: bionet.molbio.ageing Subject: Forwarded: aspartame a real danger? Date: 22 Sep 1995 05:51:53 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 255 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <43shgi$n42@news.euro.net> NNTP-Posting-Host: net.bio.net From betty@pd.org Fri Sep 22 07:57:28 1995 Date: Thu, 21 Sep 1995 14:50:10 -0400 (EDT) From: Betty Martini To: Ron Blue Subject: Re: Tinnitus FAQ Pointer (fwd) Betty Martini Domain: betty@pd.org UUCP: ...!emory!pd.org!betty ---------- Forwarded message ---------- Date: Wed, 20 Sep 1995 14:57:00 -0400 From: Betty Martini Newgroups: alt.support.tinnitus, alt.rock-n-roll.hard, alt.sci.physics.acoustics, misc.health.alternative, rec.audio.misc, rec.audio.pro, rec.music.makers, rec.music.misc, sci.med, sci.med.dentistry, sci.med.nutrition Subject: Re: Tinnitus FAQ Pointer I'm replying to several inquiries I've had about aspartame and tinnitus, including this one, because of a previous post I did explaining that aspartame triggers tinnitus. Now that didn't mean that aspartame is the only cause, of course, it simply meant that we see it frequently. Aspartame use to be in 300 products, and then 600 products, but now the patent has expired and it is now in 5000 products and climbing! Some people really live a nightmare with this problem, and are very happy to learn of this trigger because when it is from aspartame it vanishes as soon as the toxin is eliminated. Also, even if the tinnitus was from another source, it might not be easy to treat if the patient was not warned that a product they were using that triggers it by itself will aggravate the problem. And lastly, nobody should use this anyway because it is a chemical poison. It is not even a diet product. It says in the Congressional Record, S5511, May 7, 1985, the following: "Aspartame has been demonstrated to inhibit the carbohydrate-induced synthesis of the neurotransmitter serotonin (Wurtman affidavit). Serotonin blunts the sensation of craving carbohydrates and thus is part of the body's feedback system that helps limit consumption of carbohydrate to appropriate levels. Its inhibition by aspartame could lead to the anomalous result of a diet product causing increased consumption of carbohydrates." Translation: Use "diet" products sweetened with aspartame and get fat! Aspartame was discovered by a Searle chemist testing a peptic ulcer drug, and it was intended to be a "drug". It is not a food additive; consider possible reactions when mixed with other drugs. It was kept off the market for 16 years because it caused brain tumors and grand mal seizures in lab animals, and thats what it is doing to the population now - and thats just for starters. The FDA admits to 92 documented symptoms from coma to death. Diabetics who it is pushed on should be warned it has the component of methanol (wood alcohol) and this is a severe metabolic poison disastrous to the health of a diabetic, and the other components are just as dangerous. In my last post I quoted two books that link aspartame to tinnitus, and said that most people who research and write about aspartame know it triggers tinnitus. I picked up a new book yesterday called NEW CHOICES IN NATURAL HEALING EDITED BY Bill Gottlieb, Editor-in-Chief, Prevention Magazine Health Books. I happen to look up NutraSweet in this book last night just to see if it was listed and here is what it says: Page 45 "Perhaps even more significant is the possible danger of many common food additives. Aspartame, the artificial sweetener sold as NutraSweet and Equal, can cause headaches and migraines, rashes, ringing ears, depression, insomnia and loss of motor control, according to a study by the Food and Drug Administration." I'm calling attention to his remark about "ringing ears". It is a known fact that aspartame triggers tinnitus, as well as other ear problems. This book has a copyright date of 1995 by Rodale Press. After my post I got a few inquiries that wanted more information, and requested references to double-blind studies. I should warn you that the studies funded by Searle (Monsanto bought Searle in l985) are flawed and, in my opinion, useless. Dr. Arthur Hull Hayes, head of the FDA, over-ruled his own board of inquiry that said not to approve aspartame and went to work for Searle's PR firm and refused to speak to the press for 10 years. It was requested that U.S. Attorney Sam Skinner indict Searle because of these flawed studies (like rats being resurrected on paper after they were declared dead!) but instead he went to work for Searle's law firm defending the case as did the U.S. Attorney after him. The statute of limitations gave out and Searle got away with putting a poison, even though its a sweet poison, in the marketplace. Since many in this newsgroup insisted on medical documentation I notified Dr. H. J. Roberts who is considered the world expert on aspartame. He has written books on the subject and many publications that were published in peer review journals. He is a Board-certified practicing internist and an internationally known medical consultant and researcher. He is listed in Who's Who in America, Who's Who in The World, Who's Who in Science and Engineering, and The Best Doctors in the U.S. He is on the Active Staff of Good Samaritan Hospital and St. Mary's Hospital (West Palm Beach), Director of the Palm Beach Institute for Medical Research (since l964) and a member of many prestigious medical and scientific organizations - including the Endocrine Society and the American Academy of Neurology. He has authored nine acclaimed books, three being nominated for a Pulitzer Prize. I requested that Dr. Roberts write a report on aspartame and tinnitus and he most graciously forwarded me the attached, in the hope that it would warn those with tinnitus, as well as others, of the danger of using aspartame. We hope you will copy it and warn others with this problem. PROFESSIONAL OPINION OF H. J. ROBERTS, M.D., F.A.C.P., F.C.C.P. CONCERNING THE USE OF PRODUCTS CONTAINING ASPARTAME (NUTRASWEET@) BY PERSONS WITH EAR AND EQUILIBRIUM PROBLEMS It is my opinion that individuals who consume products containing aspartame, including drugs and supplements, should avoid them when no specific cause can be found for these symptoms: *"Ringing" or "buzzing" of the ears (tinnitus) ... some- times described as hissing humming or whistling *Marked intolerance to noise *Impairment or loss of hearing *Marked unsteadiness or dizziness The same precaution is reasonable for persons in whom these complaints are due to other disorders because they could be aggravated by aspartame, even in minimal amounts. These corporate-neutral suggestions are based on considerable observation, research and correspondence published in more than a score of articles and two books: * ASPARTAME (NUTRASWEET@): IS IT SAFE? (Philadelphia, The Charles Press) * SWEET'NER DEAREST: BITTERSWEET VIGNETTES ABOUT ASPARTAME (NUTRASWEET@) (West Palm Beach, Sunshine Sentinel Press, P. O. Box 17799: 1 800-814-9800: Fax 407-832-2400) I also have reviewed these and related problems in my two-cassette talk, IS ASPARTAME (NUTRASWEET@) SAFE? A MEDICAL, PUBLIC HEALTH AND LEGAL OVERVIEW --1995 (Sunshine Sentinel Press). These represent hard - won insights in the trenches of a medical practice. Patients and consumers should not be misled by the "negative" conclusions of flawed studies sponsored by vested interests. There is no bias or malice intended against any company, distributor, researcher or professional who may hold contrary views. THE ROLE OF ASPARTAME Each of these components of aspartame -- phenylalanine; aspartic acid; the methyl ester, which promptly becomes methyl alcohol or methanol -- and their multiple breakdown products after exposure to heat or during prolonged storage is potentially toxic to the brain and inner ear. These organs are uniquely vulnerable to metabolic disturbances and neurotoxins because of their unique metabolic requirements. REPRESENTATIVE CASE REPORT A 30-year-old woman drank five or more cups or glasses of aspartame beverages daily for 17 months. She experienced ringing and pain in both ears, dizziness, a severe headache, and considerable loss of hearing in the left ear by audio- metric studies. When brain tumor was ruled out by CT scans, otology and neurology consultants made the diagnosis of Meniere's disease. The patient deduced that the aspartame drinks were responsible because she could predictably reproduce these symptoms on rechallenge with them. A N O V E R V I E W In my publications, and in testimony to Congress and an FDA advisory group. I have expressed the belief that the current wholesale ingestion of aspartame products by over half the adult population constitutes a probable "imminent public health hazard." My concern is bolstered by (1) evidence that these products may play a causative or aggravating role in many other medical disorders (including headaches, dizziness, confusion, impaired vision, convulsions, and probably brain tumors), (2) the flawed nature of most "scientific" studies being used to prove the alleged safety of these products, and (3) reports of serious reactions volunteered to the FDA by over 7,300 irate consumers. In the present context, these statistics are pertinent. *In my earlier report on 551 aspartame reactors (the data base is now much larger), dizziness was a major problem in 217 (39%), tinnitus in 73 (13%), severe intolerance for noise in 47, and marked impairment in hearing in 25. *The FDA (as of August 1995) had received complaints about dizziness and problems with balance from 737 consumers, and a change in hearing from 36. *The American Tinnitus Association found that the cause of tinnitus in 18,000 suffers was unknown in one-third. These complications tend to be magnified in persons with unrecognized hypothyroidism (underactive thyroid), hypoglycemia (low blood sugar reactions), diabetes, hypertension, reactions to MSG, treatment with aspirin and other drugs that can irritate the auditory nerves, and problems associated with aging. They become compounded by the threat of falls and driving accidents. I welcome reports of such reactions, and results of the "no aspartame test", for our independent registry. A 9 page survey questionnaire can be obtained by calling (407) 832-2408, or fax 407 832-2400. I have also expressed concern that aspartame products might be accelerating Alzheimer's disease. The details appear in my just-published book, DEFENSE AGAINST ALZHEIMER'S DISEASE: A RATIONAL BLUEPRINT FOR PREVENTION (Sunshine Sentinel Press - address listed above). H. J. Roberts, M.D., F.A.C.P., F.C.C.P. @1995 I might add that we have Dr. Roberts' form by email. We also ask that you send us your case history as well and the FDA because they state that only 1% actually report serious problems. And even with them not accepting some complaints and referring others to the Aids Hotline, there were still 10,000 complaints on their April, 1995 report, almost 80% of all reports to the FDA on additives. This is a very serious problem folks. Dr. Roberts did not mention what the breakdown products are but methanol converts to formaldehyde and then formic acid (ant sting poison) causing metabolic acidosis. The phenylalanine breaks down to DKP (a brain tumor agent). So unless you are looking for free embalming I would not consume aspartame in any form. You cannot heat aspartame because of these breakdown products, but the FDA forgot they have mentioned this in the past and have now approved it for baked goods, and many times aspartame is contained in baked foods in the bakery of your local grocer as well restaurant food. Pharmacists are complaining about it being in drugs from cholesterol lowering drugs to antibiotics and many times cause the very problem the drug is suppose to cure. Be careful. The soda pop companies sent diet pop to the troops in the Persian Gulf and they sat 8 weeks in the 120 degree Arabian sun. If you want to review my report on Desert Storm Syndrome and receive other information on aspartame including the history and case histories from the auto-responder please email me for information. And please warn others. Betty Martini Operation Mission Possible Betty Martini Domain: betty@pd.org UUCP: ...!emory!pd.org!betty  From owner-ageing@net.bio.net Fri Sep 22 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!newsfeed.internetmci.com!howland.reston.ans.net!news.sprintlink.net!in2.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: mikemldvn@aol.com (MikeMldvn) Newsgroups: bionet.molbio.ageing Subject: Cancer and Suicide Among Older Men Date: 22 Sep 1995 21:48:46 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 240 Sender: root@newsbf02.news.aol.com Message-ID: <43vp1u$hli@newsbf02.news.aol.com> Reply-To: mikemldvn@aol.com (MikeMldvn) NNTP-Posting-Host: newsbf02.mail.aol.com Cancer and Suicide Among Older Men (This is a long posting concerning men, cancer, and suicide. I am also posting the message's substance to forums in America OnLine and to Internet newsgroups and mailing lists where it might be appropriate. Because of its length, I suggest that anyone intrigued by the first paragraph or so *save* the posting to disk and read it offline by word processor, when convenient. For readers' protection, I usually preface postings such as this with the caveat that I am a layman, and my expertise in medicine and all other healing arts is limited to peeling the backing off a Band-Aid. However, I was a *hotline* volunteer in a suicide prevention center for a couple of years during *Viet Nam*.) Every once in a while I am reminded that suicide is a private, silent and, at times, profound gut reaction to adversity in many men who, like me, are in their 8th decade or older. The statistics on suicide show that the suicide rate for white men increases with age, and that white men over 65 are about 4 or 5 times more likely to commit suicide than older white women. Illness, loss of wife or other life partner, family and financial difficulties, alcololism, and comparable difficulties intensify a man's urges toward wanting out. As a rule, elderly men are suspicious of suicide prevention agencies (and even distrust family and close friends when it comes to suicide), and will often resist being drawn into the *prevention* process. In no uncertain terms they feel that, "It's _my_ business and I'll take care of it." Yet, we older men do want to understand why we can feel so badly that killing ourselves is the only answer but, (pardon the pun) we wouldn't be caught dead checking a book on the subject out of the library, worse yet, being seen reading it. So unintentionally coming across a posting on old man suicide while scanning the Internet is maybe OK. *** I summarized this *professional* article a few years ago in America OnLine's *SeniorNet* because I thought it was well-written and understandable. I repeated the posting recently in the Seniornet *men's* folder in the light of recent postings there by other men in which they welcomed *health* related discussions that that didn't deride or sneer at others. Also, there had been quite a few new members since the article was last posted and discussed. Anyhow, in summary: *** An article *Suicide and Cancer in Late Life* (Vol. 41, No 12, December 1990) published in the journal *Hospital and Community Psychiatry* concerned suicide among older men who were, or suspected that they were, in advanced stages of cancer. The article's authors are Yeates Conwell, M.D., Eric D. Caine, M.D., and Kurt Olsen, Ph.D. Drs. Conwell and Caine hold professorships and Dr. Olsen holds an assistant professorship at prestigious academic or health care institutions. The article's Abstract, in full, states: *In a controlled psychological autopsy study of suicide in late life, eight cases in which the victim's belief that he had cancer played a major role in the decision to end his life, were examined. All victims were men. The majority had major affective disorders, but none had been seen in mental health care settings. Other common characteristics were an active relationship with a primary physician, numerous losses, prior experience with cancer or debilitating disease, and a rigid, self-sufficient personality. The cases illustrate the complex determinants of suicidal behavior in the elderly and suggest preventive strategies.* Physical illness is a commonly cited risk factor for suicide, especially in late life. Of the physical illnesses associated with suicidal behavior, cancer stands out. (Researcher's name) estimated that malignant neoplasm was present 20 times more often in his sample of suicide victims than expected in the general population, and (researcher) studying suicide and accident victims over age 50, found cancer was significantly more common among those who took their own lives. In addition, large scale epidemiological studies have found cancer patients to be at significantly higher risk for suicide than the general population. Cancer has a special psychological significance in our culture, where it is associated with pain, suffering, and death. The 8 men: One of the 8 was 50 years old and separated, a 71 and a 76 year old were married, and the remaining 5, in their mid to late 70s, were widowed. The psychological autopsy included interviews with relatives and friends, and reviews of medical records. Informants described the men as: Mr. A: Very robust. Strong-willed. Loved to be around people, but had a short fuse. Very domineering. --- Mr. B: Strict. Precise. Honest. Friendly independent. Quiet. Withdrawn. --- Mr. C: Self-sufficient, independent, liked to be needed. --- Mr. D: Isolated and dissatisfied. Just couldn't take any more disappointments. --- Mr. E: An extremely stoic, solid guy who never complained. Avoided conflict whenever possible. --- Mr. F: Jovial, outgoing, but couldn't make decisions. --- Mr. G: Friendly, but reserved. Obsessed with colostomy care. --- Mr. H: Conservative, emotionally constricted. Psychological diagnosis: Five of the 8 men were diagnosed as *Major depression, single episode.* One, a widower, was *Alcohol abuse, in remission.* Dependence on alcohol or other intoxicants were indicated for 3 men and 1 had no diagnosis. The men were selected for this report because of their expressed concerns about cancer. Their experience with cancer is quoted as follows: Mr. A: Pain due to cancer, fear of treatment. --- Mr. B: Physical decline associated with belief that his stomach was *loaded with cancer.* --- Mr. C: Wife died of uterine cancer in 1982; physical decline associated with belief that the colon cancer (he had in 1983) was terminal, *he was fatalistic.* --- Mr. D: Somatic preoccupation: *I might have cancer, but nobody's cutting me open.* --- Mr. E: Close friend recently died after one-year hospitalization; increasing abdominal pain; was convinced *something was seriously wrong.* --- Mr. F: First wife died of cancer in 1973; cousin died of cancer in 1987; physical decline with diarrhea; *he thought he had cancer.* --- Mr. G: Wife died of cancer in 1987; deterioration of health associated with belief that he had recurrent cancer. --- Mr. H: Wife died of cancer in 1983; discovered blood in his stool and assumed he had recurrent cancer. --- Mr. H survived an attempt at suicide and was included in the study because he was a unique source of data. Three of the cases were presented in detail: Mr. B, Mr. C, Mr. H. Case 1. Mr. B was a 77 year-old who killed himself with a gunshot to the head. His wife had died in 1969 of multiple sclerosis, for which he had provided assiduous care at home. Despite subsequent losses, including the death of his only son and a favored nephew and his daughter's diagnosis with multiple sclerosis, Mr. B functioned well socially. However, his physical state was progressively more compromised by emphysema. Mr. B stated repeatedly to his friends that he would not tolerate becoming a burden on others as his wife had been. *When I get that way,* he said, *I will take the bullet.* During his last several months of life, Mr. B became more withdrawn. After a flu-like illness, he complained increasingly of stomach and head pains, sleep and appetite disturbance, and anxiety. His friends reported that he seemed preoccupied, and he revealed to them the day before his death that he thought his stomach was *loaded with cancer.* There were no other indications of impaired reality testing. He had no prior history of suicidal behavior or psychiatric illness or concerns, and he drank infrequently. Mr. B had always avoided doctors, but with the apparent change in his health during his last weeks, he was persuaded by family and friends to see a physician. An appointment was scheduled for the day of his death. When a friend arrived at his home to take him to the appointment, she found him dead. A physical autopsy was not performed. Therefore, although Mr. B's symptoms and course were consistent with a single episode of major depression, (we) were unable to specify the relationship between his affective disorder and his physical condition. Case 2: Mr. C was a 77-year old widowed white man who had lived alone since his wife's death in 1982 after a protracted struggle with uterine cancer. After a brief and appropriate grief he began to travel, an activity he enjoyed until one year later, when he received a diagnosis of cancer of the colon. He underwent treatment but refused the recommended colostomy and developed incontinence of both urine and stool. Therefore, due to incontinence, chronic obstructive pulmonary disease, cataracts, and hearing deficits, he became increasingly withdrawn and restricted in his activities. He had no history of suicidal behavior or psychiatric contacts, and he rarely drank. Throughout life Mr. C had made such comments as, *When I get old and I can't do for myself, put a gun to my head.* During the last four months of his life he rapidly declined. He had pronounced anhedonia, anorexia, weight loss, and loss of concentration and energy. Four days before his death, he saw his primary physician at his daughter's insistence. Impressed by Mr. C's deterioration, the physician scheduled a hospital admission for further evaluation. The next day Mr. C killed himself with a gunshot to the head. His family believed that he took this action with the perception that he was terminally ill with metastatic colon cancer. An autopsy showed only pulmonary emphysema; there was no evidence of recurrent tumor. Our consensus diagnosis was major depression, single episode, without psychotic features. Case 3. Unlike the preceding subjects, Mr. H failed in his attempt to take his life. He provided the following history during an inpatient hospitalization after his suicide attempt. Mr. H was a 78-year old white man who had moved from his home to a senior citizen's apartment complex in 1986 following a series of losses -- his wife's death in 1983 from metastatic cancer, a right hemispheric stroke with residual hemiparesis, and a surgical resection for treatment of bowel cancer in 1985. He had no prior history of suicide attempts or psychiatric illness. One week before his suicide attempt, Mr. H discovered blood in his stool. Concluding that his cancer had returned and that he would die following a protracted and painful course, he carefully planned his death. On the morning of his attempt, Mr. H left his family a note and money for anticipated expenses, drove his car to a municipal parking lot, and shot himself in the left side of the chest. The bullet, which exited at the tip of the left scapula, narrowly missed his heart. He was discovered several hours later. During the subsequent hospitalization, his hematochezia was diagnosed as bleeding hemorrhoids. Mr. H was without somatic delusions and gratefully accepted the fact that there was no cancer recurrence. Discussion (excerpts): The conclusion drawn here must be considered cautiously, in light of the small sample size and retrospective methodology. In addition, we can define no appropriate subject group for direct comparison. The sample included patients with cancer and those with the unverified belief that they had the illness. Our concern is not solely whether cancer places an individual at risk for suicide, but whether the belief or perception that one has a terminal illness constitutes such a risk. We seek to understand how these individuals may have been predisposed to their apparently lethal beliefs and to recognize the implications of the cases for clinical practice and suicide prevention. A second link between suicide and the belief that one has cancer may be provided by the observation that loss and a prior experience with cancer or other debilitating chronic illness were common factors among our cases. Three of the eight subjects had a prior personal history of colon cancer (Mr. C, G, and H), and the wives of four had died of cancer (Mr. C, F, G, and H). Two others (Mr. B and E) had lost a wife or close friend to other debilitating diseases. The effects of chronic and deteriorating illnesses were, therefore, familiar to most subjects and were feared. As Mr. H stated, *I don't want to die a lingering and painful death like my wife.* The fear of cancer was far more prominent than its reality. Conclusion: (excerpted) The understanding of suicide in late life must go beyond the presence or absence of associated physical or psychiatric illness to include considerations of coping style, long-term functioning, and personal values. Physicians must not be quick to adopt the view that suicide is, in any instance, a simple matter. Rather, as these cases illustrate, suicide is a multifactorial process. Although superficially understandable, these deaths caricature *rational suicide*: the victims often were clinically depressed, and they uniformly exaggerated the medical significance of their symptoms. Early in the course of their medical school education physicians should be trained to diagnose and treat depressive disorders in late life, being particularly alert for atypical somatic presentations in previously high-functioning, non-psychiatric patients. Furthermore, our training centers should reexamine their commitment to the patient-centered approach. In this age of technological medicine and cost containment, the clinician must be alert to the latent content of our interactions with patients. To avoid the elaboration and distortion that characterized the thinking of the eight men we described, clinicians must actively elicit their patients' perceptions, apprehensions, and experiences relating to cancer and other debilitating illnesses. They must take time to explore their patients' fears regarding loss of autonomy and vigor and increase of pain. Finally, on the scale of national health policy, we must educate the general public about the relationship of depressive disorders, hypochondriasis, and suicide risk; demythologize cancer, and establish reimbursement mechanisms that value the personal, time-consuming interchanges between the primary care physician and the patient through which psychotherapeutic intervention can be made. *** From owner-ageing@net.bio.net Fri Sep 22 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!howland.reston.ans.net!vixen.cso.uiuc.edu!uchinews!news.luc.edu!news From: efasco Newsgroups: bionet.molbio.ageing Subject: RE: THINNING HAIR?....MINOXIDIL USERS?... Date: 23 Sep 1995 19:19:19 GMT Organization: TRICHOGEN Lines: 5 Message-ID: <441mjn$3dk@artemis.it.luc.edu> NNTP-Posting-Host: 147.126.58.44 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2b5 (Windows; I; 16bit) Now available in U.S. PENTADECANOIC ACID GLYCERIDE and FABAO FORMULA. World's leading treatments. #1 in Japanese clinical studies. When used alone or in combination with minoxidil, will charge up your hair growth by 300%. Featured on CNN, NEWSWEEK, NEW YORK TIMES. For free info, please call 1-800-555-8655. From owner-ageing@net.bio.net Fri Sep 22 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!news.sprintlink.net!dispatch.news.demon.net!demon!mail2news.demon.co.uk!howl.demon.co.uk From: Jenny Newsgroups: bionet.molbio.ageing Subject: Advice please Date: Sat, 23 Sep 95 07:17:01 GMT Organization: - Lines: 8 Message-ID: <811840621snz@howl.demon.co.uk> Reply-To: jenny@howl.demon.co.uk X-NNTP-Posting-Host: howl.demon.co.uk X-Newsreader: Demon Internet Simple News v1.29 I hvae been searching for newsgroups which deal with coping with the effects of ageing, dealing with elderly parents, dealing with stroke or stroke support groups, carer support groups etc. So far I have not found anything. These newsgroups may exist but my server doesn't presently carry them. Anybody know of any please? -- ***** Jenny ***** From owner-ageing@net.bio.net Fri Sep 22 23:00:00 1995 Path: biosci!UCHSC.EDU!Ed.Krug From: Ed.Krug@UCHSC.EDU (Edward Krug) Newsgroups: bionet.molbio.ageing Subject: Old grafting test of Hayflick limit Date: 23 Sep 1995 01:55:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Help. I need to track down the scientific community's verdict on the experiments where a patch of distinct skin was serially passed to young mice (?) to attempt to determine if the skin stays young if it is always attached to a young animal, or if it ages even though it's blood supply comes from a young animal. I seem to recall some discussion that there was no clear conclusion because the graft became infiltrated with younger host cells. I could find the orginal article on Medline, but finding the follow on commentary and overall conclusion is difficult. Thanks, in adance, Ed Krug From owner-ageing@net.bio.net Fri Sep 22 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!howland.reston.ans.net!vixen.cso.uiuc.edu!uchinews!news.luc.edu!news From: efasco Newsgroups: bionet.molbio.ageing Subject: RE: THINNING HAIR?....MINOXIDIL USERS?... Date: 23 Sep 1995 19:19:20 GMT Organization: TRICHOGEN Lines: 5 Message-ID: <441mjo$3ht@artemis.it.luc.edu> NNTP-Posting-Host: 147.126.58.44 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2b5 (Windows; I; 16bit) Now available in U.S. PENTADECANOIC ACID GLYCERIDE and FABAO FORMULA. World's leading treatments. #1 in Japanese clinical studies. When used alone or in combination with minoxidil, will charge up your hair growth by 300%. Featured on CNN, NEWSWEEK, NEW YORK TIMES. For free info, please call 1-800-555-8655. From owner-ageing@net.bio.net Sat Sep 23 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!howland.reston.ans.net!news.nic.surfnet.nl!news.wau.nl!usenet From: Thorsten Jabs Newsgroups: bionet.molbio.ageing Subject: Re: Oxidative stress as a cause for ageing Date: 24 Sep 1995 16:44:10 GMT Organization: fyto.wau.nl Lines: 24 Message-ID: <4441sq$qu9@Trex.IenD.wau.nl> References: NNTP-Posting-Host: flex180.dbh.wau.nl Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: qjpcbraz@cc.fc.ul.pt Jorge Braz wrote: > I am looking for any information about the reactions of oxygen >radicals (o2.-, HO., O1, H2O2) with the DNA bases especially the rate >constants and the products percentages of this reactions. > Jorge Braz Hi Jorge, could you please forward your results to me. Thanks a lot, Thorsten Dr. Thorsten Jabs email: thorsten.jabs@medew.fyto.wau.nl Dept. of Phytopathology tel: +31-8370-8-3135 (fax: -3412) Wageningen Agricultural University +31-317-48-3135 from october 95 P.O. Box 8025 NL-6700 EE Wageningen The Netherlands Plant-pathogen interactions; signal perception and transduction; oxidative burst; systemic acquired resistance; hypersensitive cell death: OML means Orchestral Manoeuvers in the Light From owner-ageing@net.bio.net Sun Sep 24 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!tank.news.pipex.net!pipex!btnet!uunet!in2.uu.net!library.erc.clarkson.edu!rpi!rebecca!gk1702 From: gk1702@csc.albany.edu (KRISHNA GOPALA S) Newsgroups: bionet.molbio.ageing Subject: EM Work! Date: 25 Sep 1995 02:43:48 GMT Organization: The University at Albany Lines: 66 Distribution: na Message-ID: <445514$f0m@rebecca.albany.edu> NNTP-Posting-Host: eve.albany.edu ******************************************************** Do You Have Work On Transmission, Scanning, Cryo- Electron Microscopes, Elemental Analysis and Image Analysis? ********************************************************* I am a Certified Electron Microscopist from V.A.Hospital, Albany, NY and I have two years experience on specimen preparation, processing for Transmission, Scanning, Cryo- Electron Microscopes, Ultramicrotomy, Photographic techniques. If you send your specimen samples, I can process them and take very nice pictures under Transmission, Scanning Electron Microscopes and also perform elemental analysis under SEM and mail them to you. I charge very less money per sample comparative to other people. I charge $50 per sample. I can process any plant, animal specimens for Electron Microscpy study. Please send $50 cash or check and specimen sample to Mr.Krishna, 248 Shakerrun Apts, Albany, NY-12205. Please E-mail your questions to: gk1702@ix.netcom.com Approximate time for specimen preparation, processing for TEM and SEM: Specimen processing time for TEM 24 Hours & SEM: Ultramicrotomy, thick, thin section slides preparation, positive, negative staining techniques, observation under Transmission electron microscope depending upon microscope time and photography techniques 1 to 2 days Specimen preparation for SEM i.e. sputter coating or sputtering or carbon coating, observing under SEM and taking pictures 1 to 2 days I need to pay for chemicals, electron microscopy time, for purchasing film for both TEM and SEM etc that is why I am charging $50 per specimen. I apologize I mentioned $10 per sample in my last posting and it is not feasible for me. P.S.: This is not scam or some thing nonsense. I completed B.S. in Biology and Chemistry, M.S. in Biology, Master of Philosophy in Biology, one year intensive training on Electron Microscopy at The School Of Electron Microscopy, Veterans Hospital, Albany, NY and I am a certified computerprogrammer. I have strong ambition to utilise my Electron Microscopy skills in the field of Biology and Medicine. This way, I can fulfil my ambition to become a diagnostic Electron Microscopist and inturn I will be useful to The School Of Electron Microscopy, V.A.Hospital, Albany, NY. ************************************************************* From owner-ageing@net.bio.net Sun Sep 24 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!swrinde!tank.news.pipex.net!pipex!news.sprintlink.net!in2.uu.net!hoho.quake.net!xdcrlab.com!user From: xdcrlab@quake.net (Mike Davis) Newsgroups: bionet.molbio.ageing Subject: Re: Oxidative stress as a cause for ageing Date: Mon, 25 Sep 1995 13:11:23 -0700 Organization: XdcrLab Lines: 20 Distribution: world Message-ID: References: NNTP-Posting-Host: xdcrlab.com X-Newsreader: Value-Added NewsWatcher 2.0b24.0+ In article , Jorge Braz wrote: > I am looking for any information about the reactions of oxygen > radicals (o2.-, HO., O1, H2O2) with the DNA bases especially the rate > constants and the products percentages of this reactions. Jorge, You might try the Oxygen Therapy web site, many abstracts and a few papers, a few hundred listings. I recall Ramasarma's '94 paper mentioned a bacterial DNA protective protein that when oxidized folded over the DNA to protect it. I maintain a link on my SpringBoard website, the location may be changing soon. -- Melatonin.Folate.Trypto,Articles,Discnt.Suplmnt.Sources,Cool.Stuf The.Buffalo.SpringBoard:> http://www.quake.net/~xdcrlab/hp.html Ultrasnd.Tchnlgy: http://www.quake.net/~xdcrlab/Ultrasound.html From owner-ageing@net.bio.net Mon Sep 25 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: lcuster@aol.com (Lcuster) Newsgroups: bionet.molbio.ageing Subject: Re: Oxidative stress as a cause for ageing Date: 25 Sep 1995 20:03:46 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 29 Sender: root@newsbf02.news.aol.com Message-ID: <447g12$r40@newsbf02.news.aol.com> References: NNTP-Posting-Host: newsbf02.mail.aol.com X-Newsreader: AOL Offline Reader I just read about oxidative DNA damage in "DNA Repair and Mutagenesis" by E. Friedberg, G. Walker, and W. Siede, 1995, ASM Press. Below are a few of the references cited in this book. Damage to the DNA bases in mammalian chromatin by hydrogen peroxide in the presence of ferric and cupric ions. Arch. Biochem. Biophys. 285:317-324, 1991. Toxic DNA damage by hydrogen peroxide through the Fenton rxn in vivo & in vitro. Science 240:1302-1309. Sites & thermodynamic quantities assoc. w/proton & metal ion interaction w/RNA, DNA, and their bases, nucleosides, and nucleotides. Chem Rev. 71:439-481. Oxidative damage to DNA in mamm. chromatin Mutat. Res 275:331-342. DNA ox damage: Its mech. & measurement in mamm. systems FEBS Lett. 281:9-19 Hope this Helps -------------------------------------------------------------------------- ---- Laura Custer, Graduate Student Chemistry Dept. The American University Lcuster@aol.com From owner-ageing@net.bio.net Mon Sep 25 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!uunet!in2.uu.net!news.compuserve.com!news.production.compuserve.com!news From: George M. <75763.12@CompuServe.COM> Newsgroups: bionet.molbio.ageing Subject: Telomeres to the rescue? Date: 26 Sep 1995 02:46:27 GMT Organization: New York University Lines: 10 Message-ID: <447pi3$qpd$2@mhadf.production.compuserve.com> What role do telomeres play in cell cycle regulation? Does telomere length determine the point at which cell death occurs? If so, is there one chromosome who acts as the "watchdog" for the cell to trigger death? Thank you in advance for your insights. -- George M. From owner-ageing@net.bio.net Mon Sep 25 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!simtel!harbinger.cc.monash.edu.au!yarrina.connect.com.au!news.mel.aone.net.au!OzEmail!usenet From: Peter Hynes Newsgroups: bionet.molbio.ageing Subject: Re: What evidence for non-ageing species? Date: 22 Sep 1995 22:16:02 GMT Organization: OzEmail Pty Ltd - Australia Lines: 51 Message-ID: <43vcj2$c40@oznet03.ozemail.com.au> NNTP-Posting-Host: slmel3p54.ozemail.com.au On Mar 16, 1995 steve@chambers.ak.planet.co.nz (Steve Chambers) wrote : >In an article in Scientific American on the very old, it reported >that after 80 there was no relationship between old age and high >death rates in humans. As I recall this topic has reared its head several times. I have two queries which appear to be loosely related to Steve's - and others'- interests. 1/ The alleged independence of death rates from chronological age in the very old. *** Is this not merely an artefact of the Gaussian Curve ? When t>2*sigma, dN/dt --> 0 as a bit of algebra or simple inspection of the curve will show. So long as a group of individuals is far from its specie's mean age of death, the death RATE looks approx constant. In human males the mean age appears to be around 70.7 yr with an SD of about 8.5 yr. (The death rate curve is approx normal.) So IF you make it past say 85, your chances of dying APPEAR to level out. Comments? 2/ An oft used index of death rates is the "Age Specific Mortality, Qx". See : Carey et al, "Slowing of Mortality Rates at Older Ages in Large Medfly Cohorts" Science Vol 458, 457-461, 16\10\92 and Curtsinger et al, "Demography of Genotypes : Failure of the Limited Lifespan Paradigm in D. melanogaster", Science 258, 461-464, 16\10\92 These guys have real good data - over 10^6 flies fed and pampered till they all kicked off from old age. Trouble is, even if you start with a million, at the end you have just a handful left. And that leads to LARGE fluctuations in the stats for the tail enders. (Small sample noise.) Bad for Qx which is supposed - in the above papers - to show departures from the Gompertz Law in a large, ageing population. *** So, who thinks these papers really do show that? who has read'em, anyway? If Qx does not validate non-Gompertzian trends, where does that leave the assertion of a slowdown in death rates among the really old? Or am I missing it altogether? Comments? *** Bye all ... PJH. From owner-ageing@net.bio.net Mon Sep 25 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news.nic.surfnet.nl!highway.LeidenUniv.nl!rulc18 From: d_quale@dds.nl (Angelo Schouten) Newsgroups: bionet.molbio.ageing Subject: Re: Oxidative stress as a cause for ageing Date: Tue, 26 Sep 95 18:04:24 GMT Organization: U-Leiden Lines: 27 Message-ID: <449fkh$88m@highway.LeidenUniv.nl> References: NNTP-Posting-Host: rulc18.leidenuniv.nl X-Newsreader: News Xpress Version 1.0 Beta #3 In article , Jorge Braz wrote: > I am looking for any information about the reactions of oxygen >radicals (o2.-, HO., O1, H2O2) with the DNA bases especially the rate >constants and the products percentages of this reactions. > Jorge Braz This is a job for a physical chemist, Jorge. Perhaps theoretical calulations have been done. It is of no use to look for rate constant(s) of hydroxyl radicals with DNA bases in certain media. (.OH) is so extremely reactive and anti-specific/selective that it virtually does not excist. It even reacts with inert gases like Xenon. Therefor the toxicity of H2O2 in Fenton RedOx coupled reactions is related to the ease at which .OH (or superoxide, depending on the reaction conditions) is "released". That is the rate determining step. H2O2 should have little genotoxicity "an sich" -as a pure substance. You could consider to do time resolved pulsed EPR (Spin ECHO experiments). All of the intermediates .OH and (O2)- and singlet/triplet O have unpaired electron spins. Inducing the radical species (photolytic: UV) and watch the EPR signals become silent/silenced. Calibrate with a known spin marker like DPPH or TEMPO(L). Continuous Wave EPR is probably to slow to detect/determine .OH species. The solvent is important as well. Excited dioxygen species like singlet oxygen live longer in apolar and aprotic media than i.g. in water. You only need 10E-12 spins to get an EPR signal. Best regards, Angelo Schouten From owner-ageing@net.bio.net Tue Sep 26 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!swrinde!emory!metro.atlanta.com!news.sprintlink.net!sjuvm!lockshin Nntp-Posting-Host: 149.68.2.20 Date: Tue, 26 Sep 1995 13:50:07 -0400 From: "LOCKSHIN, RICHARD A" Newsgroups: bionet.cellbiol,bionet.celegans,bionet.drosophila,bionet.general,bionet.molbio.ageing,bionet.neuroscience Subject: Message-ID: <26SEP95.14939704.0124@sjumusic.stjohns.edu> Sender: usenet@sjumusic.stjohns.edu Organization: St. John's University Lines: 31 Xref: biosci bionet.cellbiol:3023 bionet.celegans:592 bionet.drosophila:1417 bionet.general:17311 bionet.molbio.ageing:1994 bionet.neuroscience:10450 NYC AREA CELL DEATH CLUB MEETING WED. OCT. 11, 1995, 6:30 PM Rockefeller University, 1230 York Ave. Tower Building Room 301 6:30-7 Pizza, 7-8:30 Talks and discussion Free parking after 5PM at 66th St. & York Ave. Speakers: 1. Jonathan Tilly, Harvard Molecular Basis of Ovarian Cell Death 2. Marcel Verheij, Memorial Sloan Kettering Environmental stresses signal apoptosis through a ceramide-initiated SAPK/JNK- mediated cascade. To help plan for pizza, talk, parking, etc, if you have not previously attended, please call Dr. Zakeri's LAB at 718: 997-3429 to let them know of the number of people expected and if parking is needed. Organizer: Zahra Zakeri, Queens College, Fax 718: 997-3445 Phone 718: 997-3417 Raymond Birge Rockefeller University, Fax 212: 327-7943 Phone 212: 327-7412 email pro-temp: lockshin@sjumusic.stjohns.edu From owner-ageing@net.bio.net Tue Sep 26 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news From: JULIO KARWOSKI <102225.2745@CompuServe.COM> Newsgroups: bionet.molbio.ageing Subject: Re: Telomeres to the rescue? Date: 27 Sep 1995 06:31:13 GMT Organization: CompuServe, Inc. (1-800-689-0736) Lines: 19 Message-ID: <44ar3h$klp$1@mhadf.production.compuserve.com> References: <447pi3$qpd$2@mhadf.production.compuserve.com> George: Responding to your inquiry about telomeres I should say that Allsop & Harley have found an average critical telomere length to be 0-2kbp at the onset of senescence. Also, at onset of senescense there are still many telomere sequences left on the average but since telomeres are heterochromatic, the prevailing theory is that the heterochromatin traps the first few genetic bases which will become silent. Yes, at this point senility is still reversible according to experimental evidence by J.W.Shay and W.E.West because actual genetic sequences have not been lost just yet. Your question about a senescent factor being expressed is partially right, actually there is some evidence of an anti senescent regulatory factor expressed by those first few genes but when they become engulfed in heterochromatin they become silent and senescence will occur. This is a hot area in biology and its interesting to know that some people like yourself are interested to learn more about whats making us old. best regards, Julio Karwoski From owner-ageing@net.bio.net Wed Sep 27 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!waikato!waikato.ac.nz!hawthorn From: hawthorn@waikato.ac.nz Newsgroups: bionet.molbio.ageing Subject: Re: Telomeres to the rescue? Message-ID: <1995Sep28.170323.40864@waikato.ac.nz> Date: 28 Sep 95 17:03:23 +1200 References: <447pi3$qpd$2@mhadf.production.compuserve.com> <44ar3h$klp$1@mhadf.production.compuserve.com> Organization: University of Waikato, Hamilton, New Zealand Lines: 24 In article <44ar3h$klp$1@mhadf.production.compuserve.com>, JULIO KARWOSKI <102225.2745@CompuServe.COM> writes: > George: Responding to your inquiry about telomeres I should say > that Allsop & Harley have found an average critical telomere > length to be 0-2kbp at the onset of senescence. Also, at onset of > senescense there are still many telomere sequences left on the > average but since telomeres are heterochromatic, the prevailing > theory is that the heterochromatin traps the first few genetic > bases which will become silent. Yes, at this point senility is > still reversible according to experimental evidence by J.W.Shay > and W.E.West because actual genetic sequences have not been lost > just yet. Your question about a senescent factor being expressed > is partially right, actually there is some evidence of an anti > senescent regulatory factor expressed by those first few genes > but when they become engulfed in heterochromatin they become > silent and senescence will occur. This is a hot area in biology > and its interesting to know that some people like yourself are > interested to learn more about whats making us old. > best regards, > > Julio Karwoski ... so you inject your rat with telomerase. What happens? Dead rat? Ian H From owner-ageing@net.bio.net Sat Sep 30 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.sprintlink.net!in2.uu.net!ayrton.eideti.com!usenet From: bob chapel Newsgroups: bionet.molbio.ageing Subject: Re: RE: THINNING HAIR?....MINOXIDIL USERS?... Date: 1 Oct 1995 06:46:09 GMT Organization: Clinical Aesthetic Nursing Associates Lines: 5 Message-ID: <44ldfh$rs5@ayrton.eideti.com> References: <441mjo$3ht@artemis.it.luc.edu> NNTP-Posting-Host: annex1-8.infi-net.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: yrutman@orion.it.luc.edu I've been using pentadecanoic acid for some time and feel it is of some value but I've been unable to find literature supporting it's actions. If you could direct me to some LEGITIMATE experimental data I'd be both grateful AND likely to change suppliers.....thanks From owner-ageing@net.bio.net Sat Sep 30 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.sprintlink.net!in2.uu.net!ayrton.eideti.com!usenet From: bob chapel Newsgroups: bionet.molbio.ageing Subject: Re: DHEA Date: 1 Oct 1995 07:03:00 GMT Organization: Clinical Aesthetic Nursing Associates Lines: 13 Message-ID: <44lef4$rs5@ayrton.eideti.com> References: NNTP-Posting-Host: annex1-8.infi-net.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: drierac@deakin.edu.au Chris-You might want to take a look at the book by Ward Dean and John Morgenthaler "Smart Drugs and Nutrients" it contains a chapter on DHEA and discusses it's many demonstrated and anecdotal uses(weight loss, attenuation of alzheimers,anti-tumor,anti-aging,anti-cancer effects. It has received positive evaluations in reputable medical journals (eg Lancet 1989,2(8670)pp.1048-9 "Dehydroepiandrosterone and alzheimers disease" ) It's use in humans is considered very experimental and ,not surprisingly,it is rather controversial. Those who do use it are very strongly advised to get regular assays and to d/c it's use when their blood levels return to that of a normal 20yo. Hope this helps. If you find anything of interest do return the favor as I too am interested. Bob Chapel,MSW,RN (USA) From owner-ageing@net.bio.net Sat Sep 30 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.sprintlink.net!in2.uu.net!ayrton.eideti.com!usenet From: bob chapel Newsgroups: bionet.molbio.ageing Subject: Re: DHEA Date: 1 Oct 1995 07:03:13 GMT Organization: Clinical Aesthetic Nursing Associates Lines: 13 Message-ID: <44lefh$rvs@ayrton.eideti.com> References: NNTP-Posting-Host: annex1-8.infi-net.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: drierac@deakin.edu.au Chris-You might want to take a look at the book by Ward Dean and John Morgenthaler "Smart Drugs and Nutrients" it contains a chapter on DHEA and discusses it's many demonstrated and anecdotal uses(weight loss, attenuation of alzheimers,anti-tumor,anti-aging,anti-cancer effects. It has received positive evaluations in reputable medical journals (eg Lancet 1989,2(8670)pp.1048-9 "Dehydroepiandrosterone and alzheimers disease" ) It's use in humans is considered very experimental and ,not surprisingly,it is rather controversial. Those who do use it are very strongly advised to get regular assays and to d/c it's use when their blood levels return to that of a normal 20yo. Hope this helps. If you find anything of interest do return the favor as I too am interested. Bob Chapel,MSW,RN (USA) From owner-ageing@net.bio.net Sat Sep 30 23:00:00 1995 Path: biosci!agate!library.ucla.edu!info.ucla.edu!news.ucdavis.edu!rocky!fzcboe From: fzcboe@rocky.ucdavis.edu (Carl Boe) Newsgroups: bionet.molbio.ageing Subject: Re: What evidence for non-ageing species? Date: 1 Oct 1995 22:36:50 GMT Organization: University of California, Davis Lines: 105 Message-ID: <44n562$17l@mark.ucdavis.edu> References: <43vcj2$c40@oznet03.ozemail.com.au> NNTP-Posting-Host: rocky.ucdavis.edu X-Newsreader: TIN [version 1.2 PL2] Peter Hynes (pjhynes@ozemail.com.au) wrote: : On Mar 16, 1995 steve@chambers.ak.planet.co.nz (Steve Chambers) wrote : : >In an article in Scientific American on the very old, it reported : >that after 80 there was no relationship between old age and high : >death rates in humans. : As I recall this topic has reared its head several times. I have two : queries which appear to be loosely related to Steve's - and others'- : interests. : 1/ The alleged independence of death rates from chronological age : in the very old. : *** Is this not merely an artefact of the Gaussian Curve ? : When t>2*sigma, dN/dt --> 0 as a bit of algebra or simple inspection : of the curve will show. : So long as a group of individuals is far from its specie's mean age : of death, the death RATE looks approx constant. : In human males the mean age appears to be around 70.7 yr with an : SD of about 8.5 yr. (The death rate curve is approx normal.) : So IF you make it past say 85, your chances of dying APPEAR to : level out. : Comments? Of course, this is a statement about declining numbers at risk with age. In older ages, N is quite small and so the rate of change in absolute terms (i.e. the number of deaths occuring per unit time) is also small. The death rate, as usually defined, conditions on the average number of individuals exposed to mortality risk in the interval (cf. Qx below). Statements about mortality leveling off and non-Gompertz mortality at old age is a statement about the normalized tail behavior (i.e. dN/dt / N --> ??? ). : 2/ An oft used index of death rates is the "Age Specific Mortality, : Qx". : See : Carey et al, "Slowing of Mortality Rates at Older Ages in : Large Medfly Cohorts" : Science Vol 458, 457-461, 16\10\92 : and Curtsinger et al, "Demography of Genotypes : Failure of : the Limited Lifespan Paradigm in D. melanogaster", : Science 258, 461-464, 16\10\92 : These guys have real good data - over 10^6 flies fed and pampered : till they all kicked off from old age. Trouble is, even if you : start with a million, at the end you have just a handful left. : And that leads to LARGE fluctuations in the stats for the tail : enders. (Small sample noise.) There is always increasingly large fluctuations in the tails. The point of the million is that you can go much further into the tail region before demographic stochasticity becomes a problem. : Bad for Qx which is supposed - in the above papers - to show : departures from the Gompertz Law in a large, ageing population. For any fixed x, the s.e. of the estimated Qx will be smaller, the larger the number of flies alive at x. So to get lots alive at x, you start with huge numbers. Of course there will always be an age old enough so that you don't have very many left alive and, at that age, the estimates wil be quite uncertain. : *** : So, who thinks these papers really do show that? who has read'em, : anyway? If Qx does not validate non-Gompertzian trends, where : does that leave the assertion of a slowdown in death rates : among the really old? Well, I think the phenomenon cannot be denied, although the *reasons* for the decline as well as the extent of the levelling off in other species remain important issues for current research. Alternative explanations include cage and density effects, genetic heterogeneity of individuals. These effects could produce a leveling off in the following way. If mortality increases with density, then as time progresses, the density within a cage falls, as more and more flies die off. This leaves the oldest flies with very low density and therefore with much lower mortality levels. The argument for genetic heterogeneity is that the initial population consists of a mixture of genetically different individuals and genetic endowments strongly influence mortality rates. Thus flies with high mortality genes die off early, leaving a greater and greater proportion of flies with low mortality genes. Selection proceeds thorugh the ages so that only the most robust of flies live to old age. If the selection process is fast enough, mortality rates can decline in the aggregate (even when they are remaining constant or even increasing for all individuals). I think to a large extent these were addressed in the original articles and responses to letters that appeared later in Science. The Carey et al. article conducted three separate experiments, two of which did not involve flies caged in groups but rather in isolation. There is still "confinement" in these isolation experiments, but there is fixed density. The qualitative findings were the same as for the 1 million experiment that used grouped cages: the mortality slowdown persists across all three experiments. The experiments in Curtsinger et al. controlled for genetic variation by using pure strains of Drosophila. While genetics played a considerable role in determining longevity, within each strain the same mortality levelling off was observed. : Bye all ... PJH. --Carl Boe UC Davis p.s. disclaimer: I have a vested interest in the above as I am Jim Carey's lab at Davis.