From owner-ageing@net.bio.net Sun Mar 02 22:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.ageing Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 3 Mar 1997 02:00:12 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703031000.CAA21060@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-ageing@net.bio.net Mon Mar 03 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!newsfeeds.sol.net!europa.clark.net!news.msfc.nasa.gov!info.uah.edu!te6000.otc.lsu.edu!sp115.ocs.lsu.edu!grant From: grant@bit.csc.lsu.edu (Kevin Paul Grant) Newsgroups: bionet.molbio.ageing Subject: Roslin sheep/genetics/aging questions Date: 4 Mar 1997 11:10:47 GMT Organization: Department of Computer Science, LSU, Baton Rouge Lines: 84 Message-ID: <5fgvvn$h6m@sp115.ocs.lsu.edu> NNTP-Posting-Host: bit.csc.lsu.edu Forgive me if the material below shows a horrible ignorance of basic biochemistry. I have no training in the area. The genetic material used in the sheep cloning experiments so recently in the news was taken from cells from adult sheep. The procedure resulted in what appears to be another, normal sheep. This gives rise to some interesting questions: 1. If aging is due to cumulative damage in DNA wouldn't a cloning using DNA from the mammeries (the donor site, according to the CNN story) of an adult sheep *not* result in a normal, healthy clone sheep? 2. Are there cells ("stem" cells?) in a sheep's mammeries that are not susceptible to such cumulative damage and that were, in fact, used to get the needed DNA for these experiments? 3. Perhaps the DNA used *was* age-damaged but something about the cloning process or some natural process that takes place during the development of the fetus reverses the damage? Now if I may drift slightly for just a moment, my understanding is that: 1. The genes in DNA are covered by molecules that can prevent some of them from doing anything. 2. Every cell in the body has the same DNA but that what makes the cells different is that each type of cell has its own specific combination of "uncovered" genes that are able to operate. 3. At different times the set of "uncovered" genes in a cell can change. 4. All of this (and a lot more) is what comes under the heading of "gene expression" in genetics textbooks. Now the following question arises: Has "naked" (completely lacking in any covering molecules) DNA from a person ever been checked to see if it is the DNA itself that accumulates age-related damage? Or is it possible that it is the stuff that covers the various genes that gets damaged and it is the symptoms of *this* damage that are at least partially responsible for aging? Perhaps the DNA in sex cells (as they exist in normal fertilization) is completely "uncovered" and some part of the cloning (or fetal development) process can completely "uncover" covered DNA, removing the damaged covering material and thus allowing "adult" DNA to be used for cloning purposes without resulting in a damaged end-product. If any of this is reasonable, then perhaps a lot of what I've seen bandied about concerning telomere length and DNA damage is not as critical to the aging process as it would seem and restoring proper gene-expression would be a more profitable avenue to pursue regarding anti-aging. If this is reasonable, is there any material available about this sort of thing? Has anyone ever tried such an approach and if so what were the results? None of what I'm writing is new, I'm sure. My interest here is more along the lines of getting the answers to some questions like: 1. What are the stupid mistakes in what I've written? 2. Can anyone give me further information along the lines of what I've written (at least the parts that aren't stupid)? 3. Does the successful use of "adult" DNA in the "Roslin" sheep tell us anything about cumulative damage to genetic material as a partial explanation of the symptoms of aging?" Thanks, Kevin From owner-ageing@net.bio.net Tue Mar 04 22:00:00 1997 Path: biosci!OLINET.ISF.KIEV.UA!gerund From: gerund@OLINET.ISF.KIEV.UA (Vadim Chromiak) Newsgroups: bionet.molbio.ageing Subject: Quest fo Dr.Kirkwood's email Date: 4 Mar 1997 22:58:24 -0800 Organization: Research Center for Radiology, Kiev, Ukraine Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <331D9E4A.5C05@olinet.isf.kiev.ua> Reply-To: gerund@olinet.isf.kiev.ua NNTP-Posting-Host: net.bio.net Hello to all, Can anybody send me the e-mail address of Prof.Kirkwood. Some people in the Institute of Gerontology of Ukraine are very interested in receiving his address urgently in order to propose him a colabourative research project. Please, respond to "direct@geront.freenet.kiev.ua" (for Dr.Muradian) or to "gerund@olinet.isf.kiev.ua"(for Dr.Muradian) Many thanks in advance! Vadim Chromyak, Inst. Gerontology, Kiev, Ukraine From owner-ageing@net.bio.net Tue Mar 04 22:00:00 1997 Newsgroups: bionet.molbio.ageing Path: biosci!rutgers.rutgers.edu!news.sgi.com!howland.erols.net!cam-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.sprintlink.net!news-peer.sprintlink.net!news-pull.sprintlink.net!news.sprintlink.net!news-stk-3.sprintlink.net!NEWS!not-for-mail From: "Joseph Cannon" Subject: DHEA and athletic performance question Message-ID: <01bc28ca$dd6f6060$6d1b29cf@JCannon> Date: Tue, 04 Mar 1997 17:38:03 GMT X-Newsreader: Microsoft Internet News 4.70.1155 Lines: 11 I have read that DHEA works some of its reported benefits via inhibition of glucose -6- phosphat dehydrogenase, which is needed in a nutshell for ATP resynthesis. if this is true, would using DHEA effect athletic performance??? please reply via email. any respnonse is greately appreciated -- Joseph Patrick Cannon BS chemistry and Biology MS Exercise Physiology (May 1997) CSCS From owner-ageing@net.bio.net Tue Mar 04 22:00:00 1997 Path: biosci!agate!howland.erols.net!usenet.kornet.nm.kr!newsfeed.dacom.co.kr!usenet.dacom.co.kr!not-for-mail From: Hang-Jun Chang Newsgroups: bionet.molbio.ageing Subject: Re: Roslin sheep/genetics/aging questions Date: Wed, 05 Mar 1997 23:44:33 +0900 Organization: Dansan Public Health Center Lines: 40 Message-ID: <331D86D1.A97@chollian.dacom.co.kr> References: <5fgvvn$h6m@sp115.ocs.lsu.edu> NNTP-Posting-Host: 164.124.168.57 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win95; I) To: Kevin Paul Grant CC: iam@chollian.dacom.co.kr Kevin Paul Grant wrote: > > > > Has "naked" (completely lacking in any covering molecules) > DNA from a person ever been checked to see if it is the DNA > itself that accumulates age-related damage? Or is it possible > that it is the stuff that covers the various genes that gets > damaged and it is the symptoms of *this* damage that are at > least partially responsible for aging? > > > Perhaps the DNA in sex cells (as they exist in normal fertilization) > is completely "uncovered" and some part of the cloning (or fetal > development) process can completely "uncover" covered DNA, removing > the damaged covering material and thus allowing "adult" DNA to be > used for cloning purposes without resulting in a damaged end-product. > What a great idea it is! Kevin's idea can be summaried as follows: The cause of aging is in the altered expression of genes like a developmental process, rather than in accumulated damages to genes themselves. Should a certain damage be involved in the aging process, it may be in the molecules to regulate the gene expression. Thus, Aging may occurs as a result of altered expressions of genes by such accumulated damages. I want to suggest that DNA methylation holds some answers to your questions. Although I don't know much about it, as far as I know, DNA methylation patterns change as a cell differentiates, or ages. Perhaps DNA methylation appeares to be involved in transcription regulation. I remember the journal Cell has several good reviews on it. I hope that this discussion goes on. Is there anyboy to comment it? I am really excited by this great post. Best regards Hang-Jun Jang From owner-ageing@net.bio.net Tue Mar 04 22:00:00 1997 Path: biosci!CIS.OHIO-STATE.EDU!jj From: jj@CIS.OHIO-STATE.EDU (John Josephson) Newsgroups: bionet.molbio.ageing Subject: re: aging questions Date: 5 Mar 1997 12:48:03 -0800 Organization: LAIR, CIS, The Ohio State University Lines: 32 Sender: daemon@net.bio.net Distribution: world Message-ID: <331D8E0F.72AE@cis.ohio-state.edu> References: <331D86D1.A97@chollian.dacom.co.kr> NNTP-Posting-Host: net.bio.net > ... . > I want to suggest that DNA methylation holds some answers to your > questions. > Although I don't know much about it, as far as I know, DNA methylation > patterns change as a cell differentiates, or ages. > Perhaps DNA methylation appeares to be involved in transcription > regulation. I remember the journal Cell has several good reviews on it. > I hope that this discussion goes on. > Is there anyboy to comment it? I am really excited by this great post. > > Best regards > Hang-Jun Jang Richard Cutler has proposed what he calls the "disdifferentiation hypothesis," basically, that a big cause of the disfunctions of aging may be that differentiated cells tend to loose their specificity over time so that, for example, neurons tend to make more muscle proteins, livers make more skin proteins, etc. That they do so is supposedly an experimental fact; what is in question is whether that is indeed a deep cause of the manifestations of aging, or whether it is more an effect than a cause. (Sorry, I can't give references right now.) Presumably, too, one main way that the differentiation of cells is maintained is by methylation of DNA. (Though I think that methylation is supposedly used to do some imprinting across generations, i.e., for some genes it matter whether you got the gene from your mother or father, and that the mechanism for this sex imprinting is methylation of DNA. So perhaps just putting the nucleus of an adult cell into an egg is not enough to remove all methylation.) .. jj From owner-ageing@net.bio.net Wed Mar 05 22:00:00 1997 Newsgroups: bionet.molbio.ageing Path: biosci!rutgers.rutgers.edu!news.sgi.com!howland.erols.net!cam-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!cam-news-feed2.bbnplanet.com!shore!mv!mv.mv.com!katel From: katel@mv.mv.com (Katharine Lindner) Subject: Re: Roslin sheep/genetics/aging questions Organization: MV Communications, Inc. Message-ID: References: <5fgvvn$h6m@sp115.ocs.lsu.edu> <331D86D1.A97@chollian.dacom.co.kr> X-Nntp-Posting-Host: mv.mv.com Date: Thu, 6 Mar 1997 05:20:30 GMT Lines: 48 Hang-Jun Chang writes: >Kevin Paul Grant wrote: >> >> >> >> Has "naked" (completely lacking in any covering molecules) >> DNA from a person ever been checked to see if it is the DNA >> itself that accumulates age-related damage? Or is it possible >> that it is the stuff that covers the various genes that gets >> damaged and it is the symptoms of *this* damage that are at >> least partially responsible for aging? >> >> >> Perhaps the DNA in sex cells (as they exist in normal fertilization) >> is completely "uncovered" and some part of the cloning (or fetal >> development) process can completely "uncover" covered DNA, removing >> the damaged covering material and thus allowing "adult" DNA to be >> used for cloning purposes without resulting in a damaged end-product. >> >What a great idea it is! >Kevin's idea can be summaried as follows: >The cause of aging is in the altered expression of genes like a >developmental process, rather than in accumulated damages to genes >themselves. Should a certain damage be involved in the aging process, it >may be in the molecules to regulate the gene expression. >Thus, Aging may occurs as a result of altered expressions of genes >by such accumulated damages. >I want to suggest that DNA methylation holds some answers to your >questions. >Although I don't know much about it, as far as I know, DNA methylation >patterns change as a cell differentiates, or ages. >Perhaps DNA methylation appeares to be involved in transcription >regulation. I remember the journal Cell has several good reviews on it. >I hope that this discussion goes on. >Is there anyboy to comment it? I am really excited by this great post. Another possibility is that DNA in some cells is healthy and damaged in others. With a full organism the damage is averaged, but maybe in a single cell its likely to be either dead or reasonably healthy. Yet another speculation. Kate From owner-ageing@net.bio.net Wed Mar 05 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!newsfeeds.sol.net!europa.clark.net!cpk-news-hub1.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!news.pbi.net!news.PBI.net!nitrogen-c.themall.net!ptp105-218.themall.net From: JULIO KARWOSKI Newsgroups: bionet.molbio.ageing Subject: Re: aging questions Date: Wed, 05 Mar 1997 23:32:38 -0800 Organization: Pacific Bell Internet Services Lines: 71 Message-ID: <331E7316.554D@themall.net> References: <331D86D1.A97@chollian.dacom.co.kr> <331D8E0F.72AE@cis.ohio-state.edu> NNTP-Posting-Host: nitrogen-c.themall.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02E-KIT (Win16; U) John Josephson wrote: > > > ... . > > I want to suggest that DNA methylation holds some answers to your > > questions. > > Although I don't know much about it, as far as I know, DNA methylation > > patterns change as a cell differentiates, or ages. > > Perhaps DNA methylation appeares to be involved in transcription > > regulation. I remember the journal Cell has several good reviews on it. > > I hope that this discussion goes on. > > Is there anyboy to comment it? I am really excited by this great post. > > > > Best regards > > Hang-Jun Jang > > Richard Cutler has proposed what he calls the "disdifferentiation > hypothesis," basically, that a big cause of the disfunctions of aging > may be that differentiated cells tend to loose their specificity over > time so that, for example, neurons tend to make more muscle proteins, > livers make more skin proteins, etc. That they do so is supposedly an > experimental fact; what is in question is whether that is indeed a deep > cause of the manifestations of aging, or whether it is more an effect > than a cause. (Sorry, I can't give references right now.) Presumably, > too, one main way that the differentiation of cells is maintained is by > methylation of DNA. (Though I think that methylation is supposedly used > to do some imprinting across generations, i.e., for some genes it matter > whether you got the gene from your mother or father, and that the > mechanism for this sex imprinting is methylation of DNA. So perhaps just > putting the nucleus of an adult cell into an egg is not enough to remove > all methylation.) > > .. jj Hello Mr. J.J. and Mr Han Juang: I was somewhat surprised and pleased that somebody brought the methylation hypothesis to explain the cloning process. From what I read, these British researchers had to fertilize about 300 cells to obtain one successful fertilization. It appears that the DNA of the unsuccessful attempts was damaged in some way. Methylation is one of the major mechanisms for gene silencing and I am surprised that it is given so little publicity by those involved in aging research. The genetic "imprinting" as Mr J.J. well points out is according to some researchers an inherited pattern of distribution of methyl groups along the DNA strand. In addition to maintaining the expression or silencing of the appropriate genes, there is a loss of methyl groups with each cell division, and also due to random damage in postmitotic cells. This loss of methyl groups {C-H3} will cause some unwanted genes to be expressed, and according to several authorities will cause the appearance of scenesence. To me this theory is quite conclusive. There could be still a more basic mecanism for cellular aging but these researchers are definitely on the right track. The books I would recommend for anyone to read on the cutting edge on aging research are: genes and aging, by M.S. Kanungo understanding ageing, by Robin Holliday They give a comprehensive review of the different theories and focus an what is the most logical cause of aging at the cellular level, the inherent failure of maintenance mechanisms of the cell. For example, methylase does not repair the missing methyl groups perfectly, therefore, eventually the cell will have unwanted active genes. J.R.Smith from the University of Texas has, without mentioning methylation as a cause, discovered that unwanted proteins are expressed in aging cells. He has identified these proteins and his results have been confirmed by a different route by other researchers. They only focussed their research on those proteins which interfered with cell division. Their studies were done by cell fusion techniques. If anybody is interested in more information or the articles I have, please post. J.R. Smith also has a home page with useful information. cordially, Julio Karwoski From owner-ageing@net.bio.net Wed Mar 05 22:00:00 1997 Path: biosci!daresbury!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!gmorley From: gmorley@hgmp.mrc.ac.uk (Mr. G. Morley) Newsgroups: bionet.molbio.ageing Subject: Re: Sheep genetics ageing Date: 6 Mar 1997 14:04:36 GMT Organization: MRC Human Genome Resource Centre Lines: 15 Message-ID: <5fmitk$t18@mercury.hgmp.mrc.ac.uk> NNTP-Posting-Host: tin.hgmp.mrc.ac.uk Another possibility is that the aging process is linked to the number of normal mitochondria the individual possess per cell..... the nucleus of the adult cell was transplanted into the denucleated egg of another sheep - the mitochondria of which should all be fully functioning. Also telomerases might be active in the early embryo which are switched of in the adult (?) lengthening the telomeres and thus extending the division rate of the cell (?) But these (previous to this reply)are interesting speculations and they go to show that the cloning has already stimulated scientific discussion as well as, alas, the more excitable members of the general population. From owner-ageing@net.bio.net Thu Mar 06 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!newsfeeds.sol.net!news.maxwell.syr.edu!worldnet.att.net!howland.erols.net!torn!n1tor.istar!tor.istar!east.istar!news From: Webmaster Newsgroups: bionet.molbio.ageing Subject: Tempur-Pedic Date: 7 Mar 1997 15:30:22 GMT Organization: iSTAR internet Incorporated Lines: 17 Message-ID: <5fpcae$6ha@nr1.toronto.istar.net> NNTP-Posting-Host: news2.gtn.net Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-2 Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01Gold (Win95; I) REVOLUTIONARY PAIN RELIEVING MATTRESS Tempur-Pedic is a revolutionary comfort material, orginally developed for the space program. Because of its pressure relieving properties the Tempur-Pedic mattress effectively relieves bodily pains caused or made worse by unnatural sleeping postures. IMPROVES QUALITY OF SLEEP Test made at the Institution for Clinical and Physiological Research, Lillhagen Hospital, Gothenburg, Sweden, have demonstrated that subject turned an average of 17 times per night compared to 80-100 times on a conventional mattress. http://www.gtn.net/oxylite E-mail= oxylite@gtn.net From owner-ageing@net.bio.net Sun Mar 09 22:00:00 1997 Path: biosci!daresbury!not-for-mail From: mjfasmb Newsgroups: bionet.molbio.ageing Subject: (Fwd) Re: Sheep genetics ageing Date: 10 Mar 1997 11:41:52 -0000 Organization: Dept. Chem Eng - UMIST (FS1) Lines: 20 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5g0s20$7rn@mserv1.dl.ac.uk> X-mailer: Pegasus Mail for Windows (v2.32) MIME-Version: 1.0 Original-To: ageing@dl.ac.uk In reply to Mr. G. Morley, I'd be surprised if the mitochondria play an important role in the ageing process. My work with yeast (i'm afraid i work at the unglamorous end of the ageing field) implies that mitochondrial damage has very little effect on cellular senescence. Cheers Gordon Barker Another possibility is that the aging process is linked to the number of normal mitochondria the individual possess per cell..... the nucleus of the adult cell was transplanted into the denucleated egg of another sheep - the mitochondria of which should all be fully functioning. From owner-ageing@net.bio.net Sun Mar 09 22:00:00 1997 Path: biosci!ALUMNI.UBC.CA!BRowley From: BRowley@ALUMNI.UBC.CA (Longevity-Digest/Brian Rowley) Newsgroups: bionet.molbio.ageing Subject: English gerontologists Date: 10 Mar 1997 15:05:11 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703102305.PAA20091@unixg.ubc.ca> NNTP-Posting-Host: net.bio.net Those of you who are English gerontologists or graduate students studying the biology of aging, please reply to this post. I will be in England between April 28 and May 20, and would like to meet English gerontologists and their graduate students to chat with them informally about their (past or present) work. My purpose is purely educational; I'm not a job-seeker or spy trying to swipe unpublished ideas. I am visiting my English grandparents and wish to use the opportunity to hear about the work of as many English gerontologists as are willing to talk to me. I am Brian Rowley (BS, MS). I am currently applying to Ph. D programs at UCLA and UC Riverside to study the biology of aging (I'm being purposely vague, here). I wasn't planning on doing my Ph. D in England, but am interested in gaining contacts for possible future collaboration, and most importantly, in getting up to date on a broad range of work :-> Regards, Brian Rowley From owner-ageing@net.bio.net Tue Mar 11 22:00:00 1997 Path: biosci!GNN.COM!DSchwa1234 From: DSchwa1234@GNN.COM (Douglas Schwartz) Newsgroups: bionet.molbio.ageing Subject: Re: (Fwd) Re: Sheep genetics ageing Date: 11 Mar 1997 21:45:31 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703120545.AAA11309@mail-e2b.gnn.com> NNTP-Posting-Host: net.bio.net >From: mjfasmb > >In reply to Mr. G. Morley, I'd be surprised if the mitochondri >a play >an important role in the ageing process. My work with yeast ( >i'm >afraid i work at the unglamorous end of the ageing field) impl >ies >that mitochondrial damage has very little effect on cellular >senescence. > >Cheers >Gordon Barker Gordon, Could you go into what sorts of things you find with senescent yeast? And what (if any) methods of slowing their rate of aging are known. Thanks, --Doug Schwartz dschwa1234@gnn.com From owner-ageing@net.bio.net Tue Mar 11 22:00:00 1997 Path: biosci!daresbury!not-for-mail From: mjfasmb Newsgroups: bionet.molbio.ageing Subject: Sheep don't make beer Date: 12 Mar 1997 15:35:34 -0000 Organization: Dept. Chem Eng - UMIST (FS1) Lines: 44 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5g6ig6$q3c@mserv1.dl.ac.uk> X-mailer: Pegasus Mail for Windows (v2.32) MIME-Version: 1.0 Original-To: ageing@dl.ac.uk > Could you go into what sorts of things you find with senescent > yeast? And what (if any) methods of slowing their rate of aging are > known. > Thanks, > > --Doug Schwartz > dschwa1234@gnn.com Hi Doug, Blimey ! I'm not too sure where to begin... I guess the most interesting result is that whilst you can shorten yeast lifespan by increasing oxidative damage (by deleting SOD1 for example), senescing yeast only one division from arrest - which according to damage hypotheses are so knackered they are about to die from their mutational load - are still able to generate viable progeny. These daughters, despite inheriting an almost lethally mutated set of cellular apparatus, can divide several times and in turn give rise to perfectly healthy daughter cells ! Sounds to me like cells just lose the ability to overide their cell-cycle checkpoints. Perhaps each time a cell stalls in the cell cycle ('cos there aren't quite enough nutrients or maybe DNA damage has been detected), it doesn't quite reset the biological activity of its checkpoint-arrest signal. These signals would accumulate as the cell got older, until such a time as they prevented further division - even if the cell was otherwise fairly healthy.... what do you think ? As for methods of slowing ageing down in yeast - one of the easiest is to grow them on different carbon sources. Strains I've handled seem to increase their replicative lifespans on ethanol (as opposed to glucose) and especially galactose. I've tested other carbon sources too, and there doesn't appear to be any predictable pattern with, say, fermentable versus non-fermentable sources . Altering the expression levels of genes like LAG1 + 2, RAS2 (Jazwinski's group) and PHB1 seem able to extend yeast lifespan. Guarente's lab isolated a whole bunch of lifespan-extending genes which they called UTH1, 2, etc (for youth). I think their screen was based upon starvation tolerance. They found some pretty interesting genes with the screen - like SIR4 and HTR1 ! Cheers Gordon From owner-ageing@net.bio.net Fri Mar 14 22:00:00 1997 Path: biosci!IUNHAW1.IUN.INDIANA.EDU!TSTABLER From: TSTABLER@IUNHAW1.IUN.INDIANA.EDU ("Tim Stabler") Newsgroups: bionet.molbio.ageing Subject: Re: Meeting Date: 15 Mar 1997 09:38:20 -0800 Organization: Indiana University Northwest Lines: 33 Sender: daemon@net.bio.net Distribution: world Message-ID: <1CC71B60DF@iunhaw1.iun.indiana.edu> Reply-To: tstabler@iunhaw1.iun.indiana.edu NNTP-Posting-Host: net.bio.net The Society for In Vitro Biology (formerly the Tissue Culture Association)will be holding its 1997 Congress on In Vitro Biology in Washington, DC at the REnaissance Hotel, June 14-18, 1997. Main areas of interest incklude vertebrate, invertebrate, toxicology and plant. As a member of the Planning Committee, I can say the abstracts we reviewed for the meeting look to be excellent. The hotel is elegant and isa located about two blocks from museums and about a 15 walk from the White House. The program for the meeting is available at: http://www.sivb.org You can Email the Society at: sivb@sivb.org Although the abstract deadline is past, abstracts are still being accepted as "Hot Topics" to be published in a separate journal available at the meeting. I believe the deadline for these is April 15. If you have any questions, feel free to contact the Society or myself. Timothy A. Stabler, Ph.D. Department of Biology Indiana University Northwest Gary, IN 46408 (219)980-6718 FAX: (219)980-7125 From owner-ageing@net.bio.net Sat Mar 15 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!news-peer.gsl.net!news.gsl.net!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!news.campus.mci.net!n-f-m From: "Jan Coetzee" Newsgroups: bionet.molbio.ageing Subject: Is Hayflick's theory universal? Date: 15 Mar 1997 21:05:05 GMT Organization: CampusMCI Lines: 8 Message-ID: <01bc3184$9067d820$341166ce@coetzee_jv> References: <1CC71B60DF@iunhaw1.iun.indiana.edu> NNTP-Posting-Host: s23-pm07.atlanta.campus.mci.net X-Newsreader: Microsoft Internet News 4.70.1157 I am trying to find publications on Hayflick studies that has been done on different animals such as dogs, horses and turtles in order to see if his hypothesis is true for species other than man. If these studies has been done and there is a linear relationship between the age limit of different animals and limited cell division then his theory is universal. If not then his ideas are given to much emphasis in aging research Jan (John) Coetzee From owner-ageing@net.bio.net Mon Mar 17 22:00:00 1997 Path: biosci!worldnet.att.net!grendle From: grendle@worldnet.att.net (jim and barbara burkhart) Newsgroups: bionet.molbio.ageing Subject: implications of dolly Date: 17 Mar 1997 19:09:46 -0800 Organization: restorations ltd Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <332DC71E.59AF@worldnet.att.net> NNTP-Posting-Host: net.bio.net The cloning of the sheep has certainly opened a number of questions about the regulation of lifespan and the critical aging signal. The possibilty importance of methylation was mentioned. Alternate methylation patterns in maternal and paternal gametes have been suggested as critical for development but the cloning of the sheep clearly doesn't require gamete methylation programing. The suggestion of methylation as important to ageing is likewise defeated by the sheep. In both cases the presumed crucial methylation is a property of the "old" nucleus but development apparently proceeds normally. I believe that implicates the programming of the egg as critical. Does the ageing signal reside in the nucleus? If the ageing "clock" is nuclear, DNA damage or telomere lenth have been proposed, then Dolly is a problem. Perhaps the successful fusion contains not the odd undamaged nucleus but a nucleus that has lost genes critical to sensing ageing. It will be interesting to see if clones have an elevated cancer incidence. From owner-ageing@net.bio.net Mon Mar 17 22:00:00 1997 Path: biosci!daresbury!not-for-mail From: rattan@kemi.aau.dk (Suresh Rattan) Newsgroups: bionet.molbio.ageing Subject: Scientific hierarchy/humor Date: 18 Mar 1997 09:23:56 -0000 Lines: 28 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5glmvc$343@mserv1.dl.ac.uk> Original-To: ageing@dl.ac.uk All those scientists who study living systems are supposed to be "biologists". Yet, there is an intrinsic intellectual hierarchy which is mainly due to the changing fashions. It appears that for the last several years: Clinicians think that they are physiologists; Physiologists think that they are anatomists; Anatomists think that they are cell biologists; Cell biologists think that they are biochemists; Biochemists think that they are molecular biologists; Molecular biologists think that they are God; and what about God? Well, God does not think !!! (Got it????)... Suresh Rattan Dr. Suresh I.S. Rattan, PhD; DSc Laboratory of Cellular Ageing Department of Chemistry Aarhus University DK-8000 Aarhus - C Denmark (Phone: +45 8942 3956; Fax: +45 8619 6199) From owner-ageing@net.bio.net Mon Mar 17 22:00:00 1997 Path: biosci!daresbury!not-for-mail From: mjfasmb Newsgroups: bionet.molbio.ageing Subject: Re: implications of dolly Date: 18 Mar 1997 17:13:34 -0000 Organization: Dept. Chem Eng - UMIST (FS1) Lines: 26 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5gmifu$ak4@mserv1.dl.ac.uk> X-mailer: Pegasus Mail for Windows (v2.32) MIME-Version: 1.0 Original-To: ageing@dl.ac.uk > Date: Tue, 18 Mar 1997 13:24:41 GMT Re: implications of dolly Surely patterns of transcription can be stably maintained without having to invoke some change in the methylation state of the DNA ? Such patterns might, given the right environmental cues, alter to assume a new expression-pattern (attractor ?), during progression from embryo to adult, but needn't be the product of physically altering the DNA. Resetting to some basal / default expression pattern either through mating or electric shock (I seem to remember that's what the Rosalin people did) would then suffice to produce a viable clone... MGB x > I disagree with your dismissal of methylation's role in > aging: DNA methylation patterns are reset during early > embryogenesis. In fact, the Dolly experiment supports a > methylation theory of aging (changes irreversible in adult > cells but reversible in embryonal cells) over a mutational > theory of aging (changes irreversible no matter what). > Incidentally, there is some data suggesting that this may be > true for some cancers as well. > Jean-Pierre Issa > > grendle@worldnet.att.net (jim and barbara burkhart) wrote: From owner-ageing@net.bio.net Mon Mar 17 22:00:00 1997 Path: biosci!daresbury!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!gmorley From: gmorley@hgmp.mrc.ac.uk (Mr. G. Morley) Newsgroups: bionet.molbio.ageing Subject: Re: implications of dolly Date: 18 Mar 1997 16:18:31 GMT Organization: MRC Human Genome Resource Centre Lines: 54 Message-ID: <5gmf8n$6h3@mercury.hgmp.mrc.ac.uk> NNTP-Posting-Host: tin.hgmp.mrc.ac.uk grendle@worldnet.att.net (jim and barbara burkhart) wrote: >>The cloning of the sheep has certainly opened a number of questions >>about the regulation of lifespan and the critical aging signal. The >>possibilty importance of methylation was mentioned. Alternate >>methylation patterns in maternal and paternal gametes have been >>suggested as critical for development but the cloning of the sheep >>clearly doesn't require gamete methylation programing. The suggestion of >>methylation as important to ageing is likewise defeated by the sheep. In >>both cases the presumed crucial methylation is a property of the "old" >>nucleus but development apparently proceeds normally. I believe that >>implicates the programming of the egg as critical. >>Does the ageing signal reside in the nucleus? If the ageing "clock" is >>nuclear, DNA damage or telomere lenth have been proposed, then Dolly is >>a problem. Perhaps the successful fusion contains not the odd undamaged >>nucleus but a nucleus that has lost genes critical to sensing ageing. It >>will be interesting to see if clones have an elevated cancer incidence. Jean-Pierre Issa wrote: >I disagree with your dismissal of methylation's role in >aging: DNA methylation patterns are reset during early >embryogenesis. In fact, the Dolly experiment supports a >methylation theory of aging (changes irreversible in adult >cells but reversible in embryonal cells) over a mutational >theory of aging (changes irreversible no matter what). >Incidentally, there is some data suggesting that this may be >true for some cancers as well. >Jean-Pierre Issa I may be wrong but what I think jim and barbara burkhart were reffering to was the aspect that imprinting can play in development... I don't think all DNA Methylation patterns are removed following miosis - the damaged gene which causes Angelman syndrome in females ,(I think!) is the same as that which causes Prauder-Willie (spelling?) syndrome... I may well be wrong (as my memory is not the best) but the differences in phenotype are determined by whether the gene is inhereted from the father or the mother..(via I think differences in parent methylation patterns. I could well be wrong but I think there are other cases (such as an offspring inheriting for eg. two chromosome 11's from the father and none from the mother) where the offsprings phenotype is affected(?) The second possibility is that I am talking complete b****ks.. I would be interested if anyone out there could make some sense of this post and the relevance of methylation/demethylation in embryogenisis vs aging? Cheers! Gary Morley gmorley@rpms.ac.uk From owner-ageing@net.bio.net Mon Mar 17 22:00:00 1997 NNTP-Posting-Host: invivo.edu Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!news-xfer.netaxs.com!europa.clark.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!rill.news.pipex.net!pipex!oleane!pasteur.fr!jussieu.fr!rain.fr!invivo.edu!news From: "mjfasmb,mjfasmb@fs1.ce.umist.ac.uk"@invivo.edu Reply-To: "mjfasmb,mjfasmb@fs1.ce.umist.ac.uk"@invivo.edu Newsgroups: bionet.molbio.ageing Distribution: world Subject: implications of dolly Date: Tue, 18 Mar 1997 17:13:34 GMT Message-ID: <753795038.3189553@invivo.edu> Organization: inVivo X-Gateway: FirstClass Gateway for SMTP/NNTP (MacPPC) version 1.02 Lines: 48 > Date: Tue, 18 Mar 1997 13:24:41 GMT Re: implications of dolly Surely patterns of transcription can be stably maintained without having to invoke some change in the methylation state of the DNA ? Such patterns might, given the right environmental cues, alter to assume a new expression-pattern (attractor ?), during progression from embryo to adult, but needn't be the product of physically altering the DNA. Resetting to some basal / default expression pattern either through mating or electric shock (I seem to remember that's what the Rosalin people did) would then suffice to produce a viable clone... MGB x > I disagree with your dismissal of methylation's role in > aging: DNA methylation patterns are reset during early > embryogenesis. In fact, the Dolly experiment supports a > methylation theory of aging (changes irreversible in adult > cells but reversible in embryonal cells) over a mutational > theory of aging (changes irreversible no matter what). > Incidentally, there is some data suggesting that this may be > true for some cancers as well. > Jean-Pierre Issa > > grendle@worldnet.att.net (jim and barbara burkhart) wrote: --- Internet Message Header Follows --- Path: news.remcomp.fr!rain.fr!jussieu.fr!univ-lyon1.fr!in2p3.fr!news-ge.switch.ch!news-fra1.dfn.de!news-ber1.dfn.de!zrz.TU-Berlin.DE!fu-berlin.de!news.apfel.de!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsxfer3.itd.umich.edu!howland.erols.net!agate!news.Stanford.EDU!biosci!daresbury!not-for-mail From: mjfasmb Newsgroups: bionet.molbio.ageing Subject: Re: implications of dolly Date: 18 Mar 1997 17:13:34 -0000 Organization: Dept. Chem Eng - UMIST (FS1) Lines: 26 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5gmifu$ak4@mserv1.dl.ac.uk> X-mailer: Pegasus Mail for Windows (v2.32) MIME-Version: 1.0 Original-To: ageing@dl.ac.uk -- end **** Sent by inVivo. The BBS of Life Sciences, Paris, France, ******** mailto://info@invivo.edu ****** http://www.invivo.net ***** From owner-ageing@net.bio.net Mon Mar 17 22:00:00 1997 Path: biosci!ALUMNI.UBC.CA!BRowley From: BRowley@ALUMNI.UBC.CA (Longevity-Digest/Brian Rowley) Newsgroups: bionet.molbio.ageing Subject: Re: implications of dolly Date: 18 Mar 1997 12:13:54 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703182014.MAA04074@unixg.ubc.ca> NNTP-Posting-Host: net.bio.net mjfasmb wrote: >Surely patterns of transcription can be stably maintained without >having to invoke some change in the methylation state of the DNA ? >Such patterns might, given the right environmental cues, alter to >assume a new expression-pattern (attractor ?), during progression >from embryo to adult, but needn't be the product of physically altering >the DNA. Resetting to some basal / default expression pattern either >through mating or electric shock (I seem to remember that's what the >Rosalin people did) would then suffice to produce a viable clone... > >MGB >x Your arguments make a lot of sense. I'd like to know, though, were Dolly's telomeres (or Hayflick divisions) restored to those of a newborn lamb? It would be easy to find that out; just compare number of remaining divisions (and length of telomeres) of sheep progenitor with that of lamb clone. If the telomeres and remaining divisions were restored by cloning that would imply that rejuvenation of adult cells is possible. Brian Rowley From owner-ageing@net.bio.net Mon Mar 17 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!cs.utexas.edu!howland.erols.net!worldnet.att.net!news.alt.net!newspost1.alt.net!usenet From: jpissa@welchlink.welch.jhu.edu Newsgroups: bionet.molbio.ageing Subject: Re: implications of dolly Date: Tue, 18 Mar 1997 13:24:41 GMT Organization: Altopia Corp. - Affordable Usenet Access - http://www.alt.net Lines: 28 Message-ID: <332e9692.248069034@news.alt.net> References: <332DC71E.59AF@worldnet.att.net> Reply-To: jpissa@welchlink.welch.jhu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Newsreader: Forte Agent .99f/32.289 X-No-Archive: yes I disagree with your dismissal of methylation's role in aging: DNA methylation patterns are reset during early embryogenesis. In fact, the Dolly experiment supports a methylation theory of aging (changes irreversible in adult cells but reversible in embryonal cells) over a mutational theory of aging (changes irreversible no matter what). Incidentally, there is some data suggesting that this may be true for some cancers as well. Jean-Pierre Issa grendle@worldnet.att.net (jim and barbara burkhart) wrote: >The cloning of the sheep has certainly opened a number of questions >about the regulation of lifespan and the critical aging signal. The >possibilty importance of methylation was mentioned. Alternate >methylation patterns in maternal and paternal gametes have been >suggested as critical for development but the cloning of the sheep >clearly doesn't require gamete methylation programing. The suggestion of >methylation as important to ageing is likewise defeated by the sheep. In >both cases the presumed crucial methylation is a property of the "old" >nucleus but development apparently proceeds normally. I believe that >implicates the programming of the egg as critical. >Does the ageing signal reside in the nucleus? If the ageing "clock" is >nuclear, DNA damage or telomere lenth have been proposed, then Dolly is >a problem. Perhaps the successful fusion contains not the odd undamaged >nucleus but a nucleus that has lost genes critical to sensing ageing. It >will be interesting to see if clones have an elevated cancer incidence. From owner-ageing@net.bio.net Tue Mar 18 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!news.sgi.com!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!news.campus.mci.net!n-f-m From: "Jan Coetzee" Newsgroups: bionet.molbio.ageing Subject: Re: implications of dolly Date: 18 Mar 1997 17:47:30 GMT Organization: CampusMCI Lines: 8 Message-ID: <01bc33c4$7421a5a0$2e1166ce@coetzee_jv> References: <332DC71E.59AF@worldnet.att.net> NNTP-Posting-Host: s17-pm07.atlanta.campus.mci.net X-Newsreader: Microsoft Internet News 4.70.1157 I think dolly is going to change the direction of aging research for the better. It is time to through out old theories and bring in new ones! Jan (John) Ph.D.. coetzee_jv@peachnet.campus.mci.net From owner-ageing@net.bio.net Tue Mar 18 22:00:00 1997 Path: biosci!OLINET.ISF.KIEV.UA!gerund From: gerund@OLINET.ISF.KIEV.UA (Vadim Chromiak) Newsgroups: bionet.molbio.ageing Subject: IP address of "www.aeiveos.com" Date: 18 Mar 1997 22:01:12 -0800 Organization: Research Center for Radiology, Kiev, Ukraine Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <333009AD.55D9@olinet.isf.kiev.ua> Reply-To: gerund@olinet.isf.kiev.ua NNTP-Posting-Host: net.bio.net Dear Group, Who can give me an IP address of "Aeiveos". My Name server can't translate "www.aeiveos.com". The same is true for "Life Extension Foundation". A typical response is " doesn't have a DNS Entry". Thanks in advance, Vadim. From owner-ageing@net.bio.net Tue Mar 18 22:00:00 1997 Path: biosci!newshost.lanl.gov!usenet From: Brian Foley Newsgroups: bionet.molbio.ageing Subject: Re: implications of dolly Date: Wed, 19 Mar 1997 11:56:26 -0700 Organization: HIV Database Lines: 31 Message-ID: <333036DA.56A1@t10.lanl.gov> References: <332DC71E.59AF@worldnet.att.net> NNTP-Posting-Host: idiotype.lanl.gov Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (X11; U; SunOS 5.5.1 sun4m) The Dolly Lamb ah... Mary had a little lamb, its fleece was slightly grey, It didn't have a father, just some borrowed DNA. It sort of had a mother, though the ovum was on loan, It was not so much a lambkin, as a little lamby clone. And soon it had a fellow clone, and soon it had some more, They followed her to school one day, all cramming through the door. It made the children laugh and sing, the teachers found it droll, There were too many lamby clones, for Mary to control. No other could control the sheep, since their programs didn't vary, So the scientists resolved it all, by simply cloning Mary. But now they feel quite sheepish, those scientists unwary, One problem solved, but what to do, with Mary, Mary, Mary... From owner-ageing@net.bio.net Tue Mar 18 22:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!worldnet.att.net!news.maxwell.syr.edu!mindspring!news.mindspring.com!usenet From: lockshin@mindspring.com (Richard A. Lockshin) Newsgroups: bionet.molbio.ageing Subject: NYC Cell Death meeting April 2 corrections Date: Wed, 19 Mar 1997 20:05:08 GMT Organization: MindSpring Enterprises, Inc. Lines: 23 Message-ID: <5gph8r$n6u@camel0.mindspring.com> Reply-To: lockshin@mindspring.com NNTP-Posting-Host: ip72.an11-new-york4.ny.pub-ip.psi.net X-Server-Date: 19 Mar 1997 20:11:07 GMT X-Newsreader: Forte Free Agent 1.0.82 The Cell Death Society will meet on Wednesday, April 1, 1997 at Rockefeller University, 1230 York Avenue, Weiss Research Building (Room 301). This month's speakers will be: 1) Karen Newell, University of Vermont Coll. of Med., "Evidence for the involvement of Fas and Fas-L in multidrug resistance"; 2) Stuart Horowitz, Winthrop-University Hospital SUNY Stony Brook, "Oxidative cell death and lung injury." To help us plan for pizza and number of attendees, we ask that you call Dr. Zakeri's lab (718) 997-3429 and let them know the number of people coming for pizza, talk, and if you need parking. RSVP by Tuesday, April 1, 1997. NOTE: WE NEED FOR SUBSCRIBERS YOUR COMPLETE MAILING ADDRESS, PHONE AND FAX NUMBERS, AND EMAIL ADDRESS. If you want to be on our list, register at our site (http://celldeath-apoptosis.org) Richard A. Lockshin (lockshin@mindspring.com;lockshin@sjumusic.stjohns.edu) check out Cell Death Soc web page: http://rdz.stjohns.edu/~lockshin/index.html From owner-ageing@net.bio.net Tue Mar 18 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!arclight.uoregon.edu!news.sprintlink.net!news-peer.sprintlink.net!news.maxwell.syr.edu!mindspring!news.mindspring.com!usenet From: lockshin@mindspring.com (Richard A. Lockshin) Newsgroups: bionet.molbio.ageing Subject: NYC CELL DEATH: WED APRIL 2 Date: Wed, 19 Mar 1997 20:12:36 GMT Organization: MindSpring Enterprises, Inc. Lines: 23 Message-ID: <5gphmr$n6u@camel0.mindspring.com> Reply-To: lockshin@mindspring.com NNTP-Posting-Host: ip72.an11-new-york4.ny.pub-ip.psi.net X-Server-Date: 19 Mar 1997 20:18:35 GMT X-Newsreader: Forte Free Agent 1.0.82 The Cell Death Society will meet on Wednesday, April 2, 1997 at Rockefeller University, 1230 York Avenue, Weiss Research Building (Room 301). This month's speakers will be: 1) Karen Newell, University of Vermont Coll. of Med., "Evidence for the involvement of Fas and Fas-L in multidrug resistance"; 2) Stuart Horowitz, Winthrop-University Hospital SUNY Stony Brook, "Oxidative cell death and lung injury." To help us plan for pizza and number of attendees, we ask that you call Dr. Zakeri's lab (718) 997-3429 and let them know the number of people coming for pizza, talk, and if you need parking. RSVP by Tuesday, April 1, 1997. NOTE: WE NEED FOR SUBSCRIBERS YOUR COMPLETE MAILING ADDRESS, PHONE AND FAX NUMBERS, AND EMAIL ADDRESS. IF YOU WANT TO BE ON THE MAILING LIST, SUBSCRIBE AT HTTP:\\WWW.CELLDEATH-APOPTOSIS.ORG Richard A. Lockshin (lockshin@mindspring.com;lockshin@sjumusic.stjohns.edu) check out Cell Death Soc web page: http://rdz.stjohns.edu/~lockshin/index.html From owner-ageing@net.bio.net Tue Mar 18 22:00:00 1997 Path: biosci!ABULAFIA.ST.HMC.EDU!kkalafus From: kkalafus@ABULAFIA.ST.HMC.EDU (Ken) Newsgroups: bionet.molbio.ageing Subject: Re: implications of dolly Date: 19 Mar 1997 15:33:53 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703192339.PAA03214@abulafia.st.hmc.edu> NNTP-Posting-Host: net.bio.net Certainly the cloning of Dolly has some interesting implications for aging theory, but keep in mind: 1. The cell population used as the source of DNA in the cloning experiment was mixed (not all the same cell type). According to the science paper in which cloning was reported, the experimenters could not rule out the possibility that Dolly arose from a stem cell -- if this is the case, the cloning experiment does not tell us much about the reversibility of cellular changes associated with aging because a stem cell would presumably be protected from having undergone these changes in the first place. 2. Dolly's progenitor was only 6 years old when the cells were harvested. A sheep can live to about 30 years. Even if the source of DNA was a normal, terminally differentiated somatic cell, further information is needed before we can conclude that a molecular clock was reset at the time of Dolly's conception. It would be much more convincing if Dolly lived the maximum sheep lifespan, or even better, if Dolly were herself cloned at some advanced age. Of course, the strength of evidence for reseting Dolly's molecular clock largely depends on what sort of molecular clock you're talking about. The ability of a terminally differentiated cell to replicate itself the incredible number of times required to create an entire organism would argue very convincingly that a clock which counts divisions has been reset. Another thing to keep in mind is that the fusion of a normal cell with a denucleated egg results in an embryo which has the mitochondrea from a normal egg. This makes it impossible to rule out that aging has something to do with damage to mitochondrea. Can anyone think of a way to permanently inactivate the mitochondrea in the denucleated egg, without causing other significant damage? Perhaps cyanide? This definitely has the potential to tell us some interesting things about the aging process. It would be unfortunate if the current negative public opinions of cloning leads to a blanket ban on cloning research. --- Ken Kalafus From owner-ageing@net.bio.net Wed Mar 19 22:00:00 1997 Path: biosci!EARTHLINK.NET!AlexYCChiu From: AlexYCChiu@EARTHLINK.NET ("Alex Chiu") Newsgroups: bionet.molbio.ageing Subject: (none) Date: 20 Mar 1997 02:30:12 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703201029.CAA15577@lithuania.it.earthlink.net> NNTP-Posting-Host: net.bio.net Please visit http://home.earthlink.net/~alexycchiu/ I have invented The Eternal Life Device which allows you to keep your body physically young forever as long as you use the device every night during sleep.NEW INVENTION ALLOWS HUMANS TO LIVE FOREVER http://home.earthlink.net/~alexycchiu ACCORDING TO ALEX CHIU, PEOPLE CAN STAY YOUNG AND LIVE FOREVER BY USING HIS NEW INVENTION 'THE ETERNAL LIFE RINGS' OR 'THE ETERNAL LIFE FOOT BRACES'. The Eternal Life Rings are rings to be worn on both small fingers of a user during sleep. The Eternal Life Foot Braces are to be worn on all fingers of both feet of a user during sleep. Both devices consist simply magnets and braces which hold magnets onto the fingers of the user. Mr. Alex Chiu claims that the magnetic devices, the finger rings or the foot braces, both are believed to allow the user to stay young physically forever or to become younger! The inventor explained that the magnetic pole on the right small finger or on the right foot is opposite to the poles of the magnets on the left small finger or of the left foot. With a different pole on each side of the human body, blood circulation and electric current of the body are propelled. The speeded up blood circulation and electric current generate faster metabolism in order to fight aging process. The mentioned devices invented by Alex Chiu are believed to be able to alter aging or to keep the users young physically as long as the users wear them every night during sleep. The devices also cure many handicapnesses and deseases. Mr.Alex Chiu is an inventor who is mainly specialized in effects on human health by magnetic forces. For interested party, please email alexycchiu@earthlink.net Check out my web site http://home.earthlink.net/~alexycchiu tel 415 585 3825 From owner-ageing@net.bio.net Wed Mar 19 22:00:00 1997 Path: biosci!EARTHLINK.NET!AlexYCChiu From: AlexYCChiu@EARTHLINK.NET ("Alex Chiu") Newsgroups: bionet.molbio.ageing Subject: (none) Date: 20 Mar 1997 01:39:32 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703200940.BAA25553@spain.it.earthlink.net> NNTP-Posting-Host: net.bio.net Please visit http://home.earthlink.net/~alexycchiu/ I have invented The Eternal Life Device which allows you to keep your body physically young forever as long as you use the device every night during sleep. NEW INVENTION ALLOWS HUMANS TO LIVE FOREVER www.swiftsite.com/eternal ACCORDING TO ALEX CHIU, PEOPLE CAN STAY YOUNG AND LIVE FOREVER BY USING HIS NEW INVENTION 'THE ETERNAL LIFE RINGS' OR 'THE ETERNAL LIFE FOOT BRACES'. The Eternal Life Rings are rings to be worn on both small fingers of a user during sleep. The Eternal Life Foot Braces are to be worn on all fingers of both feet of a user during sleep. Both devices consist simply magnets and braces which hold magnets onto the fingers of the user. Mr. Alex Chiu claims that the magnetic devices, the finger rings or the foot braces, both are believed to allow the user to stay young physically forever or to become younger! The inventor explained that the magnetic pole on the right small finger or on the right foot is opposite to the poles of the magnets on the left small finger or of the left foot. With a different pole on each side of the human body, blood circulation and electric current of the body are propelled. The speeded up blood circulation and electric current generate faster metabolism in order to fight aging process. The mentioned devices invented by Alex Chiu are believed to be able to alter aging or to keep the users young physically as long as the users wear them every night during sleep. The devices also cure many handicapnesses and deseases. Mr.Alex Chiu is an inventor who is mainly specialized in effects on human health by magnetic forces. For interested party, please email alexycchiu@earthlink.net Check out my web site www.swiftsite.com/eternal tel 415 585 3825 From owner-ageing@net.bio.net Wed Mar 19 22:00:00 1997 Path: biosci!agate!spool.mu.edu!uwm.edu!newsfeeds.sol.net!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!news.campus.mci.net!n-f-m From: "Jan Coetzee" Newsgroups: bionet.molbio.ageing Subject: To the moderator of this group Date: 20 Mar 1997 17:36:35 GMT Organization: CampusMCI Lines: 4 Message-ID: <01bc3555$43463c60$291166ce@coetzee_jv> References: <5gph8r$n6u@camel0.mindspring.com> NNTP-Posting-Host: s12-pm07.atlanta.campus.mci.net X-Newsreader: Microsoft Internet News 4.70.1157 How can I post to this group? coetzee_jv@peachnet.campus.mci.net From owner-ageing@net.bio.net Wed Mar 19 22:00:00 1997 Path: biosci!EARTHLINK.NET!AlexYCChiu From: AlexYCChiu@EARTHLINK.NET ("Alex Chiu") Newsgroups: bionet.molbio.ageing Subject: (none) Date: 20 Mar 1997 02:27:04 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703201026.CAA29932@sweden.it.earthlink.net> NNTP-Posting-Host: net.bio.net Please visit http://home.earthlink.net/~alexycchiu/ I have invented The Eternal Life Device which allows you to keep your body physically young forever as long as you use the device every night during sleep.NEW INVENTION ALLOWS HUMANS TO LIVE FOREVER http://home.earthlink.net/~alexycchiu ACCORDING TO ALEX CHIU, PEOPLE CAN STAY YOUNG AND LIVE FOREVER BY USING HIS NEW INVENTION 'THE ETERNAL LIFE RINGS' OR 'THE ETERNAL LIFE FOOT BRACES'. The Eternal Life Rings are rings to be worn on both small fingers of a user during sleep. The Eternal Life Foot Braces are to be worn on all fingers of both feet of a user during sleep. Both devices consist simply magnets and braces which hold magnets onto the fingers of the user. Mr. Alex Chiu claims that the magnetic devices, the finger rings or the foot braces, both are believed to allow the user to stay young physically forever or to become younger! The inventor explained that the magnetic pole on the right small finger or on the right foot is opposite to the poles of the magnets on the left small finger or of the left foot. With a different pole on each side of the human body, blood circulation and electric current of the body are propelled. The speeded up blood circulation and electric current generate faster metabolism in order to fight aging process. The mentioned devices invented by Alex Chiu are believed to be able to alter aging or to keep the users young physically as long as the users wear them every night during sleep. The devices also cure many handicapnesses and deseases. Mr.Alex Chiu is an inventor who is mainly specialized in effects on human health by magnetic forces. For interested party, please email alexycchiu@earthlink.net Check out my web site http://home.earthlink.net/~alexycchiu tel 415 585 3825 From owner-ageing@net.bio.net Wed Mar 19 22:00:00 1997 Path: biosci!EARTHLINK.NET!AlexYCChiu From: AlexYCChiu@EARTHLINK.NET ("Alex Chiu") Newsgroups: bionet.molbio.ageing Subject: (none) Date: 20 Mar 1997 01:45:12 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703200946.BAA25736@spain.it.earthlink.net> NNTP-Posting-Host: net.bio.net Please visit http://home.earthlink.net/~alexycchiu/ I have invented The Eternal Life Device which allows you to keep your body physically young forever as long as you use the device every night during sleep. NEW INVENTION ALLOWS HUMANS TO LIVE FOREVER www.swiftsite.com/eternal ACCORDING TO ALEX CHIU, PEOPLE CAN STAY YOUNG AND LIVE FOREVER BY USING HIS NEW INVENTION 'THE ETERNAL LIFE RINGS' OR 'THE ETERNAL LIFE FOOT BRACES'. The Eternal Life Rings are rings to be worn on both small fingers of a user during sleep. The Eternal Life Foot Braces are to be worn on all fingers of both feet of a user during sleep. Both devices consist simply magnets and braces which hold magnets onto the fingers of the user. Mr. Alex Chiu claims that the magnetic devices, the finger rings or the foot braces, both are believed to allow the user to stay young physically forever or to become younger! The inventor explained that the magnetic pole on the right small finger or on the right foot is opposite to the poles of the magnets on the left small finger or of the left foot. With a different pole on each side of the human body, blood circulation and electric current of the body are propelled. The speeded up blood circulation and electric current generate faster metabolism in order to fight aging process. The mentioned devices invented by Alex Chiu are believed to be able to alter aging or to keep the users young physically as long as the users wear them every night during sleep. The devices also cure many handicapnesses and deseases. Mr.Alex Chiu is an inventor who is mainly specialized in effects on human health by magnetic forces. For interested party, please email alexycchiu@earthlink.net Check out my web site www.swiftsite.com/eternal tel 415 585 3825 From owner-ageing@net.bio.net Wed Mar 19 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!howland.erols.net!cam-news-hub1.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!news.alt.net!newspost1.alt.net!usenet From: biochemist@biochemist.com (Michael Almeida) Newsgroups: bionet.molbio.ageing Subject: Biochemist.com Date: Thu, 20 Mar 1997 17:41:34 GMT Organization: Biochemist.com Lines: 22 Message-ID: <333176c0.70460953@news.alt.net> X-Newsreader: Forte Agent .99d/32.182 Fellow Scientists, Biochemsit.com is a new site dedicated to Biochemistry and Molecular Biology. It is run and maintained by a stundent of MSU majoring in Biochemistry. The site has: 1) Online live chat room dedicated to scientific discusions 2) A message board where individuals can post question for opions related to the sciences 3) Refernces to all the commercial databases on the Internet 4) A place where individuals can post their finding/research FREE of charge Please visit the site and and make comments on what you would like added!!! thanks, Michael ALmeida http://www.biochemist.com biochemist@biochemist.com From owner-ageing@net.bio.net Wed Mar 19 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!news.sgi.com!news.maxwell.syr.edu!ix.netcom.com!compuserve.com!news.production.compuserve.com!news From: Ming Soo <110025.1742@CompuServe.COM> Newsgroups: bionet.microbiology,bionet.molbio.ageing,bionet.molbio.bio-matrix Subject: ..,WE ARE ACCEPTING NEW WRITERS Date: 19 Mar 1997 22:26:06 GMT Organization: teehoo Lines: 19 Message-ID: <5gpp5u$g0n$1@mhafc.production.compuserve.com> Xref: biosci bionet.microbiology:9364 bionet.molbio.ageing:3275 bionet.molbio.bio-matrix:878 We**are now accepting new & previously published* writers for publication. We have 3 offices/ 2 in *New York / the other in *Florida**. For ALL fiction & nonfiction/ send brief digest/ first chapter/ include a self addressed, stamped envelope: *S.A.S.E.* Poetry: send 3 poems/ *S.A.S.E. Short Stories: send brief *synopsis/ 3 pages/ *S.A.S.E. Do not send complete manuscript* unless invited to do so. *We are also accepting screenplays for TV/ Movies/ Please follow guidelines as for fiction and nonfiction. >*NYC AGENCY== >*33-29 58 Street== >*Woodside, NY 11377=- >*(718)651-8145== From owner-ageing@net.bio.net Wed Mar 19 22:00:00 1997 Path: biosci!IMB.IMB.AC.RU!koudin From: koudin@IMB.IMB.AC.RU ("Alexei R. Koudinov") Newsgroups: bionet.molbio.ageing Subject: MBF / 2 MOLGMI / RGMY Date: 20 Mar 1997 14:09:03 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.16.19970321000322.351f8d64@imb.imb.ac.ru> NNTP-Posting-Host: net.bio.net =C0 =EF=EE=F7=E5=EC=F3 =E1=FB, =E3=EE=F1=EF=EE=E4=E0, =ED=E0=EC =ED= =E5 =EE=E1=FA=E5=E4=E8=ED=E8=F2=FC=F1=FF =E8 =ED=E5 =EF=EE=E7=ED=E0=EA=EE=EC= =E8=F2=FC=F1=FF -- =F0=F3=F1=F1=EA=EE=EC=F3 =F1=EE=EE=E1=F9=E5=F1=F2=E2=F3 =F1=E8=F5 =F0=E5=F1= =F3=F0=F1=EE=E2 ? =CC=EE=E9 =F1=EF=E5=F6=E8=E0=EB=FC=ED=FB=E9 =E8=ED=F2=E5= =F0=E5=F1 -- =F1=EE=E1=F0=E0=F2=FC =E2=FB=EF=F3=F1=EA=ED=E8=EA=EE=E2 =CC=E5=E4=E8=EA=EE-=C1=E8=EE=EB=EE=E3=E8= =F7=E5=F1=EA=EE=E3=EE =D4=E0=EA=F3=EB=FC=F2=E5=F2=E0 =D0=EE=F1=F1=E8=E9=F1= =EA=EE=E3=EE =C3=EE=F1.=CC=E5=E4.=D3=ED=E8=E2=E5=F0=F1=E8=F2=E5=F2=E0, =E1=FB=E2=F8=E5=E3= =EE 2=EE=E3=EE =CC=EE=F1=EA=EE=E2=F1=EA=EE=E3=EE =CE=F0=E4=E5=ED=E0 =CB=E5= =ED=E8=ED=E0 =CC=E5=E4.=C8=ED=F1=F2=E8=F2=F3=F2=E0. =CF=EE=EB=E5=E7=ED=FB=E5 references =E1=F3=E4=F3=F2 =F1 =F0=E0=E4=EE=F1=F2= =FC=FE =EF=F0=E8=ED=FF=F2=FB. =C2=E0=F8 =C0. =CC=C1=D4-92. _____________________________ A pochemu by, gospoda, nam ne ob'edinit'sia i ne poznakomit'sia -- russkomu soobshestvu sich resursov ? Moi special'nii interes -- sobrat vypusknikov Mediko-Biologiheskogo Fakulteta Rossijskogo Gos.Med.Universiteta, byvshego 2ogo Moskovskogo Ordena Lenina Med.Instituta. Poleznye references budut s radost'iy i blagodarnost'iy priniati. Vaw A. MBF-92. From owner-ageing@net.bio.net Wed Mar 19 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!howland.erols.net!worldnet.att.net!news.alt.net!newspost1.alt.net!usenet From: biochemist@biochemist.com (Michael Almeida) Newsgroups: bionet.molbio.ageing Subject: Biochemist.com Date: Thu, 20 Mar 1997 17:41:37 GMT Organization: Biochemist.com Lines: 22 Message-ID: <333176cf.70476019@news.alt.net> X-Newsreader: Forte Agent .99d/32.182 Fellow Scientists, Biochemsit.com is a new site dedicated to Biochemistry and Molecular Biology. It is run and maintained by a stundent of MSU majoring in Biochemistry. The site has: 1) Online live chat room dedicated to scientific discusions 2) A message board where individuals can post question for opions related to the sciences 3) Refernces to all the commercial databases on the Internet 4) A place where individuals can post their finding/research FREE of charge Please visit the site and and make comments on what you would like added!!! thanks, Michael ALmeida http://www.biochemist.com biochemist@biochemist.com From owner-ageing@net.bio.net Thu Mar 20 22:00:00 1997 Path: biosci!ksc-bg.murmansk.su!peterk From: peterk@ksc-bg.murmansk.su ("P. A. Kaschoulin") Newsgroups: bionet.molbio.ageing Subject: describe needs Date: 21 Mar 1997 03:44:45 -0800 Organization: Polar-Alpine Botanical Garden-Institute Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net SUBSCRIBE From owner-ageing@net.bio.net Sat Mar 22 22:00:00 1997 Path: biosci!webtv.net!uunet!in2.uu.net!199.94.215.18!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!usc!chi-news.cic.net!cougar.olivet.edu!usenet From: IRST Newsgroups: bionet.molbio.ageing Subject: IRST Needs your help. Date: Tue, 18 Mar 1997 22:21:01 -0600 Organization: Olivet Nazarene University Lines: 108 Message-ID: <332F69AD.7E29@eggbeater.olivet.edu> NNTP-Posting-Host: r330a.chapman.olivet.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.0b2 (Win95; I) X-Priority: 3 (Normal) Dear Internet User, By now you've probably heard about the current issues pertaining to free speech and the regulation of Internet related materials. We, The Internet Regulatory Survey Team (IRST), are conducting a survey/study pertaining to these issues and are interested in your opinions. We are sending along a small 16 question survey that should take no more than five minutes of your time to complete. We ask for your participation in this study and provide us with your information. Your response will be completely anonymous. If you'd prefer to visit our Web site and fill out the form there the address is: http://eggbeater.olivet.edu/~survey/ Thank You in advance for your time! ----------------------------------------------- Internet Regulatory Survey 1. What service do you use to access the Internet? AOL Compuserve MSN Netcom Local Internet Service Provider (ISP) College or University LAN Other 2. How many times do you use Email during the week? 0-5 6-10 11-15 16-20 20+ 3. How many hours during the week do you spend on the Internet? 0-5 6-10 11-15 16-20 20+ 4. How long have you been using the Internet? 1-6 months 7-12 months 1-2 years 2-3 years 3+ years 5. How would you rate yourself as an Internet user? Beginner Intermediate Advanced Expert For questions 6-11 please indicate the level of agreement or disagreement for each. SA=Strongly Agree A=Agree N=Neutral D=Disagree SD=Strongly Disagree 6. The government should be allowed to regulate the Internet in general. SA A N D SD 7. How do you feel about laws governing material displayed on web sites? SA A N D SD 8. How do you feel about Internet service providers censoring email? SA A N D SD 9. A web site should be able to contain any information it wants no matter how vulgar, obscene, or derogatory it may be. SA A N D SD 10. Web sites should display a warning if they contain any material that may be questionable or offensive to others. SA A N D SD 11. All web sites should register their site with an Internet Censor Rating (ICR) company and display a censor rating, much like that used in television. SA A N D SD 12. Gender: Male / Female 13. Age: 14. Country: __________________ 15. Religious affiliation: Catholic Protestant Jewish Islamic Hindu Buddhist Other None 16. Political party: (U.S. Residents) Democrat Republican Independent Libertarian Other None (Non-US Residents) List political party name: _______________ ------------------------------------------------- We thank you for participation and encourage you to send us your comments and suggestions. Visit our Web site for more info Internet Regulatory Survey Team (IRST) WEB: http://eggbeater.olivet.edu/~survey/ EMAIL: survey@eggbeater.olivet.edu From owner-ageing@net.bio.net Sun Mar 23 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!newshub.csu.net!news.ironhorse.com!nntp.portal.ca!news.bc.net!rover.ucs.ualberta.ca!news.ucalgary.ca!srv1.freenet.calgary.ab.ca!hewitt From: Duane Hewitt Newsgroups: bionet.molbio.ageing Subject: Re: implications of dolly Date: Sun, 23 Mar 1997 16:57:48 -0700 Organization: Calgary Free-Net Lines: 29 Message-ID: References: <199703182014.MAA04074@unixg.ubc.ca> NNTP-Posting-Host: hewitt@srv1.freenet.calgary.ab.ca Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII To: Longevity-Digest/Brian Rowley In-Reply-To: <199703182014.MAA04074@unixg.ubc.ca> On 18 Mar 1997, Longevity-Digest/Brian Rowley wrote: > Your arguments make a lot of sense. I'd like to know, though, were > Dolly's telomeres (or Hayflick divisions) restored to those of a newborn > lamb? It would be easy to find that out; just compare number of remaining > divisions (and length of telomeres) of sheep progenitor with that of lamb > clone. If the telomeres and remaining divisions were restored by cloning > that would imply that rejuvenation of adult cells is possible. > Brian Rowley There seem to be two possibilities: 1) The early embryo has some telomerase activity which restores the telomeres. This would mean that adult cells could be rejuvenated in their telomeres. 2) The cell that the successful clone was derived from had long telomeres and that was why it was able to be cloned. Some stem cells in adults have detectable telomerase activity and it is quite possible that the cell that Dolly arose from was one of these and therefore is a relatively "rare" occurence. Duane Hewitt Immortality, Inc. http://immortality.org From owner-ageing@net.bio.net Sun Mar 23 22:00:00 1997 Path: biosci!agate!spool.mu.edu!uwm.edu!cs.utexas.edu!swrinde!howland.erols.net!newsxfer3.itd.umich.edu!newsfeed.internetmci.com!news.campus.mci.net!n-f-m From: "Jan Coetzee" Newsgroups: bionet.molbio.ageing Subject: Re: implications of dolly Date: 24 Mar 1997 01:24:01 GMT Organization: CampusMCI Lines: 24 Message-ID: <01bc37f2$0ccff5a0$4e1166ce@coetzee_jv> References: <199703182014.MAA04074@unixg.ubc.ca> NNTP-Posting-Host: s19-pm08.atlanta.campus.mci.net X-Newsreader: Microsoft Internet News 4.70.1157 Duane Hewitt writes: "There seem to be two possibilities: 1) The early embryo has some telomerase activity which restores the telomeres. This would mean that adult cells could be rejuvenated in their telomeres. 2) The cell that the successful clone was derived from had long telomeres and that was why it was able to be cloned. Some stem cells in adults have detectable telomerase activity and it is quite possible that the cell that Dolly arose from was one of these and therefore is a relatively "rare" occurence." There is a third possibility! Telomeres may have nothing to do with cell division in these particular cells. The telomere hypotheses is not universally true. Jan(John) From owner-ageing@net.bio.net Sun Mar 23 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!news.ironhorse.com!nntp.portal.ca!van-bc!n1van.istar!van.istar!west.istar!ott.istar!istar.net!tor.istar!east.istar!news1.istar.ca!news From: jpuderer@magi.com (James Puderer) Newsgroups: bionet.molbio.ageing Subject: Re: implications of dolly Date: 24 Mar 1997 04:06:49 GMT Organization: iSTAR Internet Incorporated Lines: 47 Message-ID: <5h4ukp$goc@news.istar.ca> References: <199703182014.MAA04074@unixg.ubc.ca> <01bc37f2$0ccff5a0$4e1166ce@coetzee_jv> NNTP-Posting-Host: ts33-04.ott.istar.ca X-Newsreader: Forte Free Agent 1.0.82 "Jan Coetzee" wrote: >Duane Hewitt writes: >"There seem to be two possibilities: >1) The early embryo has some telomerase activity which restores the >telomeres. This would mean that adult cells could be rejuvenated in their >telomeres. >2) The cell that the successful clone was derived from had long telomeres >and that was why it was able to be cloned. Some stem cells in adults have >detectable telomerase activity and it is quite possible that the cell that >Dolly arose from was one of these and therefore is a relatively "rare" >occurence." >There is a third possibility! >Telomeres may have nothing to do with cell division in these particular >cells. The telomere hypotheses is not universally true. As far as I know there is an awful lot of evidence pointing to telemeres being genetic clocks. It will be interesting to see what effects the possibility of shortened telomeres has on Dolly. In order to determine if telomeres do in fact play an important role in the aging process it may be important to know if the adult genetic material used to clone Dolly, was at anytime subject to telomerase activity. Which comes to my question. Does anyone know if female gametes are telomerase active? Sperm are apparantly telomarase active and it would make sence for ova to be telomarase active as well. Also, if the adult genetic material was in fact subject to telomarase activity how much would this telomarase activity extend to telomeres? It seems likely that even if the adult genetic material was subject to telomarase activity Dolly's telomeres will probably not be as long as telomeres occuring in an uncloned lamb, but then I'm just speculating. Regards, James Puderer. From owner-ageing@net.bio.net Sun Mar 23 22:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsxfer3.itd.umich.edu!portc01.blue.aol.com!audrey01.news.aol.com!not-for-mail From: djx1@aol.com (DJX1) Newsgroups: bionet.molbio.ageing Subject: Re: (none) Date: 24 Mar 1997 15:19:54 GMT Organization: AOL http://www.aol.com Lines: 13 Message-ID: <19970324151900.KAA06857@ladder01.news.aol.com> References: <199703201029.CAA15577@lithuania.it.earthlink.net> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com Hahahahahahhahahahahahahahahahahha! HHHAhaahahahahaahhahahahahahhahahhah!! Hahahahahahhaahah Hahahahh.... Whew! I needed a good laugh. Thanks, Alex. DX From owner-ageing@net.bio.net Sun Mar 23 22:00:00 1997 Path: biosci!daresbury!not-for-mail From: mjfasmb Newsgroups: bionet.molbio.ageing Subject: Sheep stuff... Date: 24 Mar 1997 20:53:03 -0000 Organization: Dept. Chem Eng - UMIST (FS1) Lines: 28 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5h6pjf$n5i@mserv1.dl.ac.uk> X-mailer: Pegasus Mail for Windows (v2.32) MIME-Version: 1.0 Original-To: ageing@dl.ac.uk > Hi Brian, > > Your arguments make a lot of sense. I'd like to know, though, were > > Dolly's telomeres (or Hayflick divisions) restored to those of a newborn > > lamb? It would be easy to find that out; just compare number of remaining > > divisions (and length of telomeres) of sheep progenitor with that of lamb > > clone. If the telomeres and remaining divisions were restored by cloning > > that would imply that rejuvenation of adult cells is possible. > > Brian Rowley > > Interesting question. I sincerely hope someone is > planning / attempting to find out how many P.D. Dolly's cells are > capable of. The length of her telomeres would be easy enough to > ascertain. Don't know if Dolly's `mother' is still alive, or > whether tissue samples taken at the same time as the `Dolly-biopsy' > were frozen, but if would be nice to find out if there is a > difference in telomere length between mother and clone cells. > > My guess is that in response to some maternal factor, or as a result > of manipulations to Dolly's original adult nucleus, some basal > embryonic expression pattern was re-established - including > expression of telomerase. There does appear to be a telomere length monitoring pathway (recent Nature paper) in cells capable of expressing telomerase - I imagine it would correct for any shortening detected... > (?) > MGB From owner-ageing@net.bio.net Tue Mar 25 22:00:00 1997 Path: biosci!BIOLOGY.LSA.UMICH.EDU!ijohn From: ijohn@BIOLOGY.LSA.UMICH.EDU (Isaac John) Newsgroups: bionet.molbio.ageing Subject: (none) Date: 26 Mar 1997 09:47:07 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe From owner-ageing@net.bio.net Fri Mar 28 22:00:00 1997 Path: biosci!agate!howland.erols.net!ix.netcom.com!enews.sgi.com!news.corp.sgi.com!news.sgi.com!news-out.internetmci.com!newsfeed.internetmci.com!news.campus.mci.net!n-f-m From: "Jan Coetzee" Newsgroups: bionet.molbio.ageing Subject: evolution and aging Date: 28 Mar 1997 22:06:43 GMT Organization: CampusMCI Lines: 10 Message-ID: <01bc3bc4$4daddd00$721166ce@coetzee_jv> NNTP-Posting-Host: s25-pm09.atlanta.campus.mci.net X-Newsreader: Microsoft Internet News 4.70.1157 Has it occurred to anyone that aging may be the consequences of "evolution" taking place in the body. For example take hair follicles. Assume that every one are born with two kinds of follicles. Normal and gray pigmented. With time subtle changes in the bodies chemistry cause the gray pigment follicles to dominate. Finally you end up with gray hair. If this is true for most cells in the body this idea could explain aging. Jan(John) From owner-ageing@net.bio.net Fri Mar 28 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!cs.utexas.edu!news.tamu.edu!news From: Dawn Newsgroups: bionet.molbio.ageing Subject: Re: evolution and aging Date: Sat, 29 Mar 1997 09:19:56 -0600 Organization: Texas A&M Univ., College of Medicine, Dept of Medical Biochemistry and Genetics Lines: 20 Message-ID: <333D331C.24AE@bigfoot.com> References: <01bc3bc4$4daddd00$721166ce@coetzee_jv> Reply-To: DawnC@bigfoot.com NNTP-Posting-Host: modem-0670.rns.tamu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit NNTP-Posting-Date: 29 Mar 1997 15:18:17 GMT X-Mailer: Mozilla 3.0 (Win95; I) Jan Coetzee wrote: > > Has it occurred to anyone that aging may be the consequences of "evolution" > taking place in the body. For example take hair follicles. Assume that > every one are born with two kinds of follicles. Normal and gray pigmented. > With time subtle changes in the bodies chemistry cause the gray pigment > follicles to dominate. Finally you end up with gray hair. If this is true > for most cells in the body this idea could explain aging. > > Jan(John) While many people have proposed an evolutionary significace to aging (may be an anti-cancer mechanism, etc.), THAT is not why we get grey hair (i.e., we are not born with two kinds of hair follicles and one begins to dominate.) -- -Dawn- Time is the best teacher. Unfortunately, it kills all of its students. Visit: http://members.aol.com/CappSci Animal Lovers check out http://members.tripod.com/~DawnC From owner-ageing@net.bio.net Sat Mar 29 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!www.nntp.primenet.com!nntp.primenet.com!newsfeed.nacamar.de!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsxfer3.itd.umich.edu!newsfeed.internetmci.com!news.campus.mci.net!n-f-m From: "Jan Coetzee" Newsgroups: bionet.molbio.ageing Subject: Re: evolution and aging Date: 30 Mar 1997 02:41:19 GMT Organization: CampusMCI Lines: 6 Message-ID: <01bc3cb3$d27dbec0$351166ce@coetzee_jv> References: <01bc3bc4$4daddd00$721166ce@coetzee_jv> <333D331C.24AE@bigfoot.com> NNTP-Posting-Host: s24-pm07.atlanta.campus.mci.net X-Newsreader: Microsoft Internet News 4.70.1157 Some times a young person have an occasional gray hair. How do you know we are not born with more than one kind of follicle? Babies (white) are often born with blond hair just to lose the hair color later on. What about animals. Some have white coats in the Winter. Jan(John) From owner-ageing@net.bio.net Mon Mar 31 23:00:00 1997 Path: biosci!TENET.EDU!dashley From: dashley@TENET.EDU (Don Ashley) Newsgroups: bionet.molbio.ageing Subject: Senior numbers Date: 1 Apr 1997 03:38:30 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 74 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net from the newsgroup: FACTS YOU SHOULD KNOW ABOUT RETIREMENT Did you know that over 100 years ago, the first retirement act was established? In Germany, General Otto Von Bismarck set the age of retirement at 65. Back then, only two percent of the population lived to retire! Nowadays, the fastest-growing segment of the population is age 80 and over--we'll be enjoying retirement for 15 or more years, not just two or three. Retirement definitely isn't what it use to be! Whether we devote that time to our families, travel, hobbies or second careers, we all want to be able to live out those years in comfort and with the knowledge that we have enough for our needs. A longer retirement is both the good news and the bad news. It is fortunate to live a longer, healthier life, but where will the income come from when we're ready to retire? An average American in 1992, with an average household income of $35,000, received only 40% of his or her income from Social Security. The rest came from personal assets, private pensions, earnings and other sources. Sources of Retirement Income 40% Social Security 10% Private Pensions 9% Government Employee Pensions 17% Earnings 21% Personal Assets 3% Other 100% Total Source: Social Security Administration, 1992. As the table indicates, you can expect to have to earn at least 38% (earnings and personal assets) of your retirement income from personal assets, private pensions, earnings and other sources. According to a 1993 Department of Labor Survey, of every 1000 people age 65, the following situations exist: * 450 are dependent on their relatives. * 280 rely entirely on public charity, welfare or Social Security. * 220 must continue to work. * 50 have enough money to meet their needs. * Of these 50, only ten have enough money to provide the financial independence that retirement should bring. YOU MAY SPEND A THIRD OF YOUR LIFE IN RETIREMENT Workers are retiring earlier. Longer life spans and earlier, healthier retirements mean you may end up spending 15, 20, even 25 or more years in retirement. It also means that there is more danger than ever of out-living your income. INFLATION Another important reason you need to plan and save for retirement is inflation. Inflation simply means that the cost of goods and services generally rises over time, as shown by the Consumer Price Index (CPI). This measurement of inflation is stated as a yearly percentage and, according to the Department of Labor, has averaged approximately 5.4% from 1965 through 1994. For example, a loaf of bread costing $1.00 today would cost $1.05 a year from now. In 20 years, that same loaf of bread would cost about $2.86, assuming that inflation continues to average 5.4%. In other words, your money will not go as far in the future as it does today.