From owner-arab-gen@hgmp.mrc.ac.uk Wed Mar 1 16:51:53 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 14E7D17A85; Wed, 1 Mar 2000 16:51:45 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 746AA17A7C; Wed, 1 Mar 2000 16:51:43 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: hilson@evry.inra.fr (Pierre Hilson) Subject: postdoc fellowship in Evry, France Date: 1 Mar 2000 13:21:48 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <200003011321.OAA29000@evry.inra.fr> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk A call for applications has just been released for postdoctoral fellowships on the Genopole campus of Evry (south of Paris, France). Candidates can be of any nationality but MUST be fluent in French because of the application procedure. The announcement reads as follows in French. ================================== Bourse post-doctorale en Génomique Végétale (INRA, Genopole, Evry) Le site Génopole d'Evry vient d'émettre un appel d'offre pour des bourses post-doctorales. Dans ce cadre, l'Unité de Recherche en Génomique Végétale de l'INRA à Evry (Dir. M. Caboche) cherche un(e) candidat(e) qui désire développer un projet concernant l'analyse du transcriptome de la plante modèle Arabidopsis thaliana, via l'exploitation de puces à ADN. Les bourses sont de 12 mois renouvelables. La date limite de dépôt des candidatures est le 24 mars 2000. Chaque candidature doit être présentée par l'intermédiaire de la structure d'accueil sous la forme d'un dossier rédigé par le candidat. Veuillez nous contacter à l'adresse suivante si cette annonce vous intéresse. Pierre Hilson URGV-INRA 2, rue Gaston Crémieux C.P. 5708 91057 EVRY Cedex Tel : 33 1 60 87 45 17 - Fax : 33 1 60 87 45 10 e-mail : hilson@evry.inra.fr --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Mar 1 16:52:46 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id AEEDA17A92; Wed, 1 Mar 2000 16:52:36 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 2BCA917A85; Wed, 1 Mar 2000 16:52:33 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: J.Leonard@sussex.ac.uk (Janette Leonard) Subject: Medium for vertical plates Date: 1 Mar 2000 14:30:47 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear all, I have a question regarding the best medium to use for vertically positioned petri-dish studies using Arabidopsis. These are for use in ecological/physiological studies concerning heterogeneous nutrient conditions. I am mainly looking at root morphology and architecture and would therefore like a clear-ish medium to enable effective scanning of the plates. I hear conflicting comments as to whether Phytagel, Agar or Agarose are the most appropriate. Could anyone provide suggestions as to which is the most suitable medium for my work? Also, if anyone out there is working in a similar field and would like to make contact, please email me! Thank-you in anticipation, Janette Leonard ***************************** Janette Leonard School of Biological Sciences University of Sussex Falmer Brighton BN1 9QG Email: J.Leonard@sussex.ac.uk Telephone: 01273 606 755 ext. 2754 Fax: 01273 678 433 --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Mar 1 16:53:26 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id E961E17AA0; Wed, 1 Mar 2000 16:53:17 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 6F43817A92; Wed, 1 Mar 2000 16:53:16 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: jacksond@cshl.org (Dave Jackson) Subject: Postdoc positions available Date: 1 Mar 2000 15:35:00 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <38BD3881.79E4CBA8@cshl.org> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Postdoctoral Positions Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (Received 02/15) Two postdoctoral positions are available in the lab of Dr. David Jackson to study genetic and molecular aspects of shoot meristem function in maize and Arabidopsis. The first position is to study cell-to-cell trafficking of the KNOTTED1 homeodomain protein using Arabidopsis as a model system. Imaging of GFP fusions will be used to study this novel pathway of cell-to-cell communication; experience in confocal microscopy and/or Arabidopsis genetics preferred. The second position is to characterize mutations in maize ear development, including the fasciated ear2 gene that we have recently cloned. Experience in maize genetics and/or general molecular biology skills preferred. Cold Spring Harbor Lab offers state-of-the-art facilities for plant genetics, imaging, and genomics. Please make inquiries to jacksond@cshl.org, telephone 516-367-8467. CSHL is situated on the north shore of Long Island, one hour from New York City. Competitive salary and housing benefits are offered. See our Web site http://www.cshl.org/. --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Mar 1 16:54:06 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 2C61217AB1; Wed, 1 Mar 2000 16:53:57 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 5204917AA0; Wed, 1 Mar 2000 16:53:53 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: faramarz@cns.bu.edu ("Faramarz Valafar") Subject: METMBS 2000 DEADLINE EXTENSION (mARCH 6) Date: 1 Mar 2000 16:16:01 -0000 Organization: Boston University Message-ID: <89jfna$elp$1@news3.bu.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk The 2000 International Conference on Mathematics and Engineering Techniques in Medicine and Biological Sciences (METMBS'2000) (E-mail submissions are encouraged) June 26 - 29, 2000 Monte Carlo Resort, Las Vegas, Nevada, USA Call for Papers/Abstracts Recent advances in computer technology have provided the tools and the environment to study, analyze, and better understand complex systems. This technological development has enabled researchers to collect and analyze massive amounts of data to a scale previously not possible. The impact of this technology is now being felt in the medical field and in the biological sciences. In recent years, research in interdisciplinary areas such as Bioinformatics and computer assisted medical decision-making has dramatically intensified. METMBS'2000 aims to provide a platform for researchers to present and discuss recent breakthroughs in this area. The METMBS'2000 Conference will be held concurrently (i.e., same location and dates) with the International Conference on Parallel and Distributed Processing Techniques and Applications (PDPTA'2000), the International Conference on Imaging Science, Systems, and Technology (CISST) and the International Conference on Artificial Intelligence (IC-AI). You are invited to submit a one-page abstract or a draft paper of about 4 pages, and/or a proposal to organize a technical session (see below for submission information). All accepted submissions will be published in the conference proceedings. THE NAMES OF TECHNICAL SESSION CHAIRS WILL APPEAR AS ASSOCIATE EDITORS ON THE COVER OF THE CONFERENCE PROCEEDINGS. SCOPE Topics of interest include, but are not limited to, the following: o Bioinformatics: This includes informatics techniques in genomics gene sequencing, gene pattern discovery, gene pattern-function studies, and other genomics related studies). o Data mining in medicine and biological sciences. o Pattern recognition in medicine and biological sciences. o Signal processing in medicine and biological sciences (e.g. biomedical signal processing, etc.) o Image processing in medicine and biological sciences (e.g. biomedical image processing, biomedical imaging, etc.) o Medical decision-making. o Medical Physics. o Biomedical Engineering. o Biomedical Electronics. o Biosignal interpretation. o Any application of computers in Medicine and biological sciences (protein structure-function analysis, drug and protein design, molecular modeling and simulation, etc.) o Application of information technology in biomedicine (e.g. medical database management, information retrieval and use of computers in hospitals) o Application of Computational Intelligence (artificial neural networks, fuzzy logic, and evolutionary computing) in medicine and biological sciences o Medical and bio-computing. o Computer-based medical systems (automation in medicine, etc.) o Recent history (1990-1999) of Mathematics and engineering techniques in medicine and biological sciences, and what to expect during the next decade (2000-2009); New horizons. Review articles) o Other aspects and applications relating to technological advancements in medicine and biological sciences. SUBMISSION OF PAPERS Prospective authors are invited to submit three copies of their one-page abstract or draft paper (about 4 pages) to F. Valafar (address is given below) by March 6, 2000. E-mail and Fax submissions are also acceptable. The length of the Camera-Ready papers (if accepted) will be limited to 7 pages. Papers must not have been previously published or currently submitted for publication elsewhere. The abstract and the first page of the draft paper should include: title of the paper, name, affiliation, postal address, E-mail address, telephone number, and Fax number for each author. The first page should also include the name of the author who will be presenting the paper (if accepted) and a maximum of 5 keywords. PROPOSAL FOR ORGANIZING TECHNICAL SESSIONS Each technical session will have at least 6 paper presentations. The session chairs will be responsible for all aspects of their sessions, including soliciting papers, reviewing, selecting, ... The names of session chairs will appear as Associate Editors in the conference proceedings. After the conference, some sessions will be considered for publication in appropriate journals as Special Issues with the session proposer as the Guest Editor of the journal. Proposals to organize technical sessions should include the following information: name and address (+ E-mail) of the proposer, title of session, a 100-word description of the topic of the session, and a short description on how the session will be advertised (in most cases, session proposers solicit papers from colleagues and researchers whose work is known to the session proposer). Mail your proposal to F. Valafar (address is given below); E-mail submissions are preferred. EVALUATION PROCESS Papers will be evaluated for originality, significance, clarity, and soundness. Two researchers in the topical area will referee each paper. The Camera-Ready papers will be reviewed by one person. PUBLICATION The conference proceedings will be published by CSREA Press (ISBN). The proceedings will be available at the conference. Some accepted papers will also be considered for journal publication (soon after the conference). ORGANIZERS/SPONSORS A number of university faculty members and their staff, in cooperation with the Monte Carlo Resort (Conference Division, Las Vegas), will be organizing the conference. The conference will be sponsored by Computer Science Research, Education, & Applications Press (CSREA: USA Federal EIN # 58-2171953) in cooperation with research centers, international associations, international research groups, and developers of high-performance machines and systems. The complete list of sponsors and co-sponsors will be available at a later time. The last conference's sponsors included: CSREA, the National Supercomputing Center for Energy and the Environment - DOE, The International Association for Mathematics and Computers in Simulation, The International Technology Institute (ITI), The Java High Performance Computing research group, Korea Information Processing Society, World Scientific and Engineering Society, Sundance Digital Signal Processing Inc., the Computer Vision Research and Applications Tech., and more. LOCATION OF CONFERENCE The conference will be held in the Monte Carlo Resort Hotel, Las Vegas, Nevada, USA. This is a mega hotel with excellent conference facilities and over 3000 rooms. The hotel is minutes from the Las Vegas airport with free shuttles to and from the airport. This hotel has many vacation and recreational attractions, including: waterfalls, casino, spa, pools & kiddie pools, sunning decks, Easy River water ride, wave pool with cascades, lighted tennis courts, health spa (with workout equipment, whirlpool, sauna,...), arcade virtual reality game rooms, nightly shows, snack bars, a number of restaurants, shopping area, bars, ... Many of these attractions are open 24 hours a day and most are suitable for families and children. The negotiated hotel's room rate for conference attendees is very reasonable ($79 + tax) per night (no extra charge for double occupancy) for the duration of the conference. The hotel is within walking distance from most other Las Vegas attractions (major shopping areas, recreational destinations, fine dining and night clubs, free street shows, and more). For the benefit of our international colleagues: the state of Nevada neighbors with the states of California, Oregon, Idaho, Utah, and Arizona. Las Vegas is only a few driving hours away from other major cities and attractions, including: Los Angeles, San Diego, Phoenix, the Grand Canyon, and more. EXHIBITION An exhibit is planned for the duration of the conference. We have reserved 20+ exhibit spaces. Interested parties should contact F. Valafar (address is given below). All exhibitors will be considered to be the co-sponsors of the conference. IMPORTANT DATES March 6, 2000 (Monday): One-page Abstracts or Draft papers (about 4 pages) due April 3, 2000 (Monday): Notification of acceptance May 1, 2000 (Monday): Camera-Ready papers & Preregistration due June 26 - 29, 2000: METMBS'2000 Conference Proposals to organize technical sessions should be submitted as soon as possible. All accepted papers are expected to be presented at the conference. MEMBERS OF PROGRAM & ORGANIZING COMMITTEES The Program Committee is currently being formed. Those interested in joining the Program Committee should e-mail F. Valafar (faramarz@cns.bu.edu ) the following information: Name, affiliation and position, complete mailing address, e-mail address, tel/fax numbers, a short biography together with research interests. OTHER INFORMATION Last year PDPTA, CISST, and IC-AI had research contributions from over 44 countries (over 900 participants from all over the world.) To make this a more complete suite of conferences, we have added METMBS. METMBS will also have a strong international flavor and offers its participants an introduction to a wide range of related interdisciplinary subjects through the other three conferences. CONFERENCE CONTACT: Faramarz Valafar Cognitive and Neural Systems Boston University 677 Beacon Street Boston, MA 02215 Tel: (617) 353-5134 Fax: (617) 353-7755 E-mail: Faramarz@cns.bu.edu --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Mar 1 18:56:55 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id A265D17A98; Wed, 1 Mar 2000 18:56:48 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 088E017A58; Wed, 1 Mar 2000 18:56:44 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: bsp541@bangor.ac.uk ("G.Merkouropoulos") Subject: Bleached areas on the leaves: III (Responses) Date: 1 Mar 2000 17:40:36 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hi, About ten days ago I send two messages to this archive asking whether there are any ideas about the bleached areas I observe on the periphery of the leaves of the Arabidopsis plants I grow. The replies I have collected are the following: Maybe its nutrient stress of some sort, deficiency or toxicity. Grant ------------------------------- Is your room really windy? Excessive airflow can burn off the edges of arabidopsis leaves. josh ------------------------------- I don't know what your husbandry conditions are, but we've found that Arabidopsis is susceptable to photo-bleaching when grown in light levels much in excess of about 300 microEinsteins, and particularly when grown under constant illumination rather than light/dark cycles. You might check your light levels. Mark ------------------------------- If you are talking about dry, dead, white patches (as opposed to otherwise healthy-looking tissue like in an albino sectoring mutant), it may be thrips. These are very hard to find unless you look very closely (and they move, so they won't be where the white patches are). Another possibility--when the Weed Science class used a colleague's watering cans to water with Liberty herbicide, her plants showed bright, white sectoring. Is anyone else using your watering cans? Allan ------------------------------- Temperatures might be too high. We see temperature stress above 23 C. Grant ------------------------------- We found that the edges of some fully expanded leaves became yellow when they touched the surface of the soil especially if the soil was too wet. As you are growing your plants in propagators or plastic bags I imagine the humidity is very high and there is condensation on some of the leaves. We grow our plants in disposable, square plastic pots where each pot is approx 4 x 4 cm. The compost was Fisons M3 and are the pots are watered from below using capillary matting. The pots are just the right size so that as the leaves expand they are supported by the edge of the pot and don't come into contact with the soil. We grow our plants under short days so the leaves can get quite large. The problem with yellowing became very marked when we grew plants in larger pots as the tips of the mature leaves would curl down. Smaller pots also gave problems as the plants became too large given the fairly extended growth period (6 weeks) Steve ------------------------------- Sounds like your conditions are fine. The only other possibility that occurs to me is that it may just be a natural part of the lifecycle of the ecotype you're using. I've been taking a look at nitrogen allocation patterns in Arabidopsis in a sort of tangent to my Ph.D. research (so the data aren't as clean as they would be if they were collected for this purpose) and one interpretation of what I see is that once the inflorescence starts growing, it becomes the primary nitrogen sink in the plant--to the point that the rosette appears to decrease its nitrogen content. I doubt if the plant is actually deallocating resources away from the rosette, but it's possible that the inflorescence sink is so strong that even the nitrogen that would be spent on tissue maintenance in the rosette is being drawn into the infloresence. Thus, the bleaching may be the result of normal protein degredation in the rosette not being repaired once the infloresence gets going. Mark ------------------------------- 1. Arabidopsis is essentially a shade plant, and whilst 170 microE is low, it may still be high enough to cause problems, particularly if coupled with another stress. 2. If the spots you describe are randomly, you may be seeing nutrient deficiency symptoms brought on by low soil oxygen. Are you keeping the soil too moist, and hence anaerobic? Try adding vermiculite to your compost mixture. 3. Are you certain you aren't just seeing signs of senescence of rosette leaves after the onset of flowering? Senescence in rosette leaves follows a clear pattern, and in Arabidopsis, runs from tip to base, and the region around the main veins remains green longest. Liz ------------------------------- Thanks again to all of you who replied to my query. G. Merkouropoulos bsp541@bangor.ac.uk --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Mar 2 00:21:42 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 9CF2E17B3E; Thu, 2 Mar 2000 00:21:38 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 4E61A17A5C; Thu, 2 Mar 2000 00:21:37 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: minsange@eden.rutgers.edu (Minsang Lee) Subject: GFP construct for plant mitochondrial targeting Date: 1 Mar 2000 21:07:29 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hello, everybody. I'm working on protein targeting in Arabidopsis thaliana. But I don't have positive control protein for mitochondrial targeting. I was wondering if anyone has the transformation construct GFP for plant mitochodrial targeting, and I could use it. If anyone help me, I'll really appreciate it. Thanks. --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Mar 2 00:22:30 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 64CC517B49; Thu, 2 Mar 2000 00:22:15 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 0BFEC17B3E; Thu, 2 Mar 2000 00:22:10 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: normand.brissonNOnoSPAM@umontreal.ca.invalid (Normand Brisson) Subject: Postdoctoral position available Date: 1 Mar 2000 22:52:01 -0000 Organization: http://www.remarq.com: The World's Usenet/Discussions Start Here Message-ID: <01f92a8a.838bc1d9@usw-ex0102-016.remarq.com> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Postdoctoral Position University of Montreal, Montreal, Canada A postdoctoral position is available immediately to work on the development of a new functional genomic approach to identify and characterize protein-protein interactions in plants. This approach is based on a Protein fragment Complementation Assay (PCA) developed recently in prokaryotes and mammalian cells (see Pelletier et al., PNAS 95, 12141-6 (1998); Pelletier et al., Nature Biotechnology 17, 683-690 (1999). The candidate will develop transient expression assays in protoplasts and tissue explants and will be in charge of plant transformation. The candidate will also use and develop fluorescence-based assays in protoplasts and in whole plant tissues. A strong background in plant tissue culture is required and preference will be given to candidates having also experience with cell biology techniques. The annual salary will be from $27,000 to $32,000 (CDN) depending on experience, and the initial appointment will be for one year with a possibility of renewal for two additional years. Please send (preferably via e-mail) your application with curriculum vitae including the names, phone numbers, and e-mail addresses of three references to Dr. Normand Brisson, Department of Biochemistry, University of Montreal, PO Box 6128, Station Centre-ville, Montreal Qc, Canada H3C 3J7. normand.brisson@umontreal.ca. * Sent from RemarQ http://www.remarq.com The Internet's Discussion Network * The fastest and easiest way to search and participate in Usenet - Free! --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Mar 2 16:31:46 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 864A017B0E; Thu, 2 Mar 2000 16:31:30 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id B09F717AFA; Thu, 2 Mar 2000 16:31:24 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: altpeter@ipk-gatersleben.de ("Dr. Fredy Altpeter") Subject: Postdoc Position Plant Molecular Biology EC funded Date: 2 Mar 2000 07:12:41 -0000 Organization: IPK Gatersleben Message-ID: <38BE12EF.23B18CAE@ipk-gatersleben.de> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Postdoc Position Plant Molecular Biology EC funded AG Gene Transfer IPK Gatersleben, Germany The IPK Gatersleben is a public research centre, with the objective to carry out basic and applied research in the field of molecular plant breeding (http://www.ipk-gatersleben.de). The Gene Transfer Group at the IPK-Gatersleben (http://www.ipk-gatersleben.de/englisch/abtl-ags/ipkengt.htm) has efficient nuclear transformation systems for the production of transgenic cereals and grasses in place. The selected candidate for this immediate opening will work in an international team focussing on the incorporation of quality traits in cereals and grasses, using genetic transformation technology and exchange research findings with multiple collaborators in an european network.. The immediate opening will be funded by the European Community for a period of 34 months at the scale BAT-IIa-OST. Development of a plastid transformation system for a selected monocot crop will build on extensive monocot tissue culture and transformation expertise and experience with biolistic plastid transformation in tobacco. The selected candidate will mainly focus on the construction of vectors for the site directed integration of transgenes through homologous recombination and the analysis of transgenic plants. We expect extensive experience with standard molecular biology techniques. Experience with plant tissue culture and -transformation is a plus. Gatersleben is located in the centre of Germany, close to the Harz mountains and the historic city of Quedlinburg. Please send your CV, list of references and intended starting date preferably by e-mail to: Dr. Fredy Altpeter, Gene Transfer Group, IPK Gatersleben Corrensstr. 3, 06466 Gatersleben, Germany e-mail: altpeter@ipk-gatersleben.de Fax: +49 39482 5139 --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Mar 2 23:59:51 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 4275217B00; Thu, 2 Mar 2000 23:59:40 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 9F8CA17AFB; Thu, 2 Mar 2000 23:59:37 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: zjchen@acs.tamu.edu ("Z. Jeffrey Chen") Subject: Rescue of an Arabidopsis plant Date: 2 Mar 2000 22:47:43 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Arabidopsis growers, I have an Arabidopsis trangenic plant that has interesting phenotypes but is male-sterile and possibly female sterile. I wonder if anyone has tried to grow Arabidopsis by cutting or some other ways to vegetatively propagate the plant. Thank you, Jeff Z. Jeffrey Chen Assistant Professor Genetics Program & Soil and Crop Sciences Department Texas A&M University College Station, TX 77843-2474 Phone: (979) 845-3773, Fax -0456 --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Mar 3 16:06:45 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 4137017B15; Fri, 3 Mar 2000 16:06:33 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 2B29E17AF3; Fri, 3 Mar 2000 16:06:30 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: dweigel@ems.salk.edu (Detlef Weigel) Subject: NAASC runoff Date: 3 Mar 2000 00:09:01 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <38BF0295.4BAD7966@ems.salk.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Colleagues, We have only received few votes on the runoff between Peter McCourt, John Bowman and Phil Benfey for the NAASC (North American Arabidopsis Steering Committee). You have one more day to vote for ONE of the three by emailing NAASC@smtp.salk.edu Detlef Weigel and Jeff Dangl, for the NAASC --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Mar 3 16:07:21 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 22C2217B21; Fri, 3 Mar 2000 16:07:05 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 8001B17B15; Fri, 3 Mar 2000 16:07:01 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: larcher@ensam.inra.fr (Marièle Larcher) Subject: looking for hormone hypersensitive mutants Date: 3 Mar 2000 09:49:37 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hi everyone I'm looking for mutants containing reporter genes useful for detecting specifically exogenous phytohormones in low concentration. The phytohormones are IAA, gibberrelin, and cytokinin. Could you advice me about the the mutant choice and where i can find it. Many thanks Mariele LARCHER Mariele Laboratoire des Symbioses Tropicales et Mediterraneennes TA 10/J Campus de Baillarguet 34398 Montpellier cedex 5 France tel: 0467593801 poste 4221 fax: 0467593802 --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Mar 3 17:04:12 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id AC4BB17B2F; Fri, 3 Mar 2000 17:04:07 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 45A2517B03; Fri, 3 Mar 2000 17:04:06 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: Michiel.VanLookerenCampagne@aventis.com ("Michiel Van Lookeren Campagne") Subject: Post-doc Position on Ovule Development Date: 3 Mar 2000 16:15:58 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Post-doc Position on Ovule Development Aventis CropScience N.V. (formerly Plant Genetic Systems) is a leading plant biotechnology company located in Gent, Belgium. We are seeking a dedicated, creative and effective Post-Doctoral Scientist with expertise in ovule development to strengthen the Reproductive Biology Research Department. The temporary position is full-time for a period of 3 years. The ideal candidate should be a cell biologist with a strong training in female reproductive development in both monocots and dicots. Experience with confocal microscopy, classical histology, ovule clearing techniques, and in situ hybridization are desired. Aventis CropScience N.V. offers an excellent compensation and benefits package. Send curriculum vitae and names of two references to Dr. Michiel M. van Lookeren Campagne, Team Leader Reproductive Biology, Aventis CropScience N.V., Jozef Plateaustraat 22, B-9000 Gent, Belgium. Phone: +32 9 235 85 31. Fax: +32 9 224 06 94. E-mail: michiel.vanlookerencampagne@aventis.com --- From owner-arab-gen@hgmp.mrc.ac.uk Mon Mar 6 16:10:23 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 64C3217A73; Mon, 6 Mar 2000 16:10:14 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 5D3B617A43; Mon, 6 Mar 2000 16:10:10 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: curator@arabidopsis.org Subject: New/Updated Arabidopsis DNA 04-Mar-2000 Date: 4 Mar 2000 14:12:27 -0000 Organization: The Arabidopsis Information Resource Message-ID: <200003041411.HAA21984@cleveland.ncgr.org> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Arabidopsis researchers, The following information is the number of Arabidopsis entries in GenBank that are new or were updated in the last week. These sequences have been updated in TAIR and are available for searching using TAIR BLAST and FASTA at: http://arabidopsis.org/blast/ http://arabidopsis.org/cgi-bin/fasta/TAIRfasta.pl New sequences : 0 Updated sequences : 0 Updated annotation : 0 --- From owner-arab-gen@hgmp.mrc.ac.uk Mon Mar 6 16:11:13 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id C8FCD17AB7; Mon, 6 Mar 2000 16:11:03 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id A3ADB17A73; Mon, 6 Mar 2000 16:11:01 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: "Vellanoweth's Lab" Subject: Chromosome 5 Date: Sat, 04 Mar 2000 12:22:25 -0800 Organization: CSUnet Message-ID: <38C17081.321172E5@calstatela.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk We are anxiously awaiting more sequence from chromosome 5. does anyone know who is working on sequencing chromo 5 or where I might find that information? Thanks, Janel Wheeler California State University, Los Angeles jwheele@calstatela.edu From owner-arab-gen@hgmp.mrc.ac.uk Mon Mar 6 16:12:29 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id E222517ACC; Mon, 6 Mar 2000 16:12:13 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id F13D417AB7; Mon, 6 Mar 2000 16:12:10 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: J.Leonard@sussex.ac.uk (Janette Leonard) Subject: Seed transfer techniques query Date: 6 Mar 2000 11:43:43 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear all, I am currently setting up a model system using Arabidopsis in agar to study considerations in the ecology and physiology of heterogeneous nutrient conditions and their effects. My plates are vertically positioned and a few cms of agar cut out of the top (as per Forde & Zheng). Could anyone tell me the best way to transfer seeds to these cut agar surfaces so they grow across the agar (or do seedlings have to be transferred for this type of growth)? Thanks in anticipation, Janette Leonard ***************************** Janette Leonard School of Biological Sciences University of Sussex Falmer Brighton BN1 9QG Email: J.Leonard@sussex.ac.uk Telephone: 01273 606 755 ext. 2754 Fax: 01273 678 433 --- From owner-arab-gen@hgmp.mrc.ac.uk Mon Mar 6 16:13:13 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 3883617ACC; Mon, 6 Mar 2000 16:13:05 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 62AC617AEF; Mon, 6 Mar 2000 16:13:03 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: kkorth@comp.uark.edu (Ken Korth) Subject: Postdoc position--Molec. Plant:Insect Interactions Date: 6 Mar 2000 15:39:17 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <3.0.6.32.20000306094217.007a5c70@comp.uark.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Postdoctoral Position Molecular Plant:Insect Interactions University of Arkansas, Fayetteville, AR A postdoctoral position is available to conduct research on plant gene induction in response to insect damage. The candidate will participate in a functional genomics project, carried out in collaboration with the Samuel Roberts Noble Foundation, aimed at genetic characterization of Medicago truncatula. The project could also involve other plant species. A Ph.D. with experience in plant molecular biology is required. Desirable skills include experience in differential screening, microarrays, cloning, and analysis of sequence and expression data. Experience in plant:insect interactions would be beneficial. Salary is commensurate with experience. Located near the Ozark Mountains, Fayetteville combines a small, college-town atmosphere with a cosmopolitan. Applications will be accepted until a suitable candidate is found. Send a letter of application, vitae and three letters of reference to: Dr. Ken Korth (kkorth@comp.uark.edu), Department of Plant Pathology, 217 Plant Science Building, University of Arkansas, Fayetteville, AR 72701. The University of Arkansas (www.uark.edu) is an equal opportunity/affirmative action institution. ********************************* Ken Korth Dept. of Plant Pathology 217 Plant Science Building University of Arkansas Fayetteville, AR 72701 Phone: (501) 575-5191 FAX: (501) 575-7601 email: kkorth@comp.uark.edu --- From owner-arab-gen@hgmp.mrc.ac.uk Mon Mar 6 18:50:55 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 629DC17A89; Mon, 6 Mar 2000 18:50:47 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 3E0D017A80; Mon, 6 Mar 2000 18:50:43 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: klimczak@akkadix.com (Les Klimczak) Subject: US-CA-SD position in microarray data analysis Date: 6 Mar 2000 16:52:39 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <38C3E240.E741D02B@akkadix.com> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Data Analyst, Expression Profiling - #AKK-066 Will contribute to ongoing database and data mining efforts with special emphasis on the management and analysis of gene expression profiling. Will be responsible for storage, extraction, and analysis of microarray and other profiling data. Will interface with lab scientists conducting expression profiling experiments. Requires familiarity with relational databases (Oracle, MS SQL Server), programming in Perl/Java, and various data mining approaches (clustering, multivariate statistics, neural networks), preferably as applied to biological data. Recent Ph.D. or M.Sc. with several years of experience. Applicants should send a cover letter indicating the job number (found next to job title) of interest and a CV describing research experience and names of three references to: Akkadix Corporation HR Department 11099 North Torrey Pines Road, Suite 200 La Jolla, CA 92037, USA Fax: (858) 625-0158 E-mail: admin@akkadix.com -- --------------------------------------------------------------------- Les Klimczak, Dr. rer. nat. | Tel.: (858) 646-8241 Senior Scientist | FAX: (858) 625-0158 Akkadix Corporation, S.160 | mailto:klimczak@akkadix.com 11099 North Torrey Pines Road | http://www.akkadix.com/lkl.html La Jolla, CA 92037 | --------------------------------------------------------------------- --- From owner-arab-gen@hgmp.mrc.ac.uk Tue Mar 7 01:38:44 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id D21BA17AAA; Tue, 7 Mar 2000 01:38:38 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 8C2B317AA9; Tue, 7 Mar 2000 01:38:37 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: kunst@interchange.ubc.ca (Ljerka Kunst) Subject: Post-doctoral Position in PLANT BIOTECHNOLOGY/BIOCHEMISTRY Date: 6 Mar 2000 22:55:02 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <3.0.1.32.20000306145414.006fce18@pop.interchange.ubc.ca> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk University of British Columbia and Linnaeus Plant Sciences are seeking a highly motivated individual to join a dynamic international team of researchers at the leading edge of oilseed engineering. The successful candidate will be based in Vancouver, a vibrant, modern city with a mild climate and excellent recreational opportunities, recently voted number one in a UN survey of the world's major cities. Candidates should have a strong background in molecular biology and biochemistry with an emphasis on the molecular genetic manipulation of plants. Experience in the field of lipid biosynthesis and metabolism will be advantageous, as will knowledge of genomics and protein expression in yeast. Applicants must be able to demonstrate a record of excellence in research with a minimum of 2 years postdoctoral experience. Excellent communication skills are essential. Salary will be competitive and commensurate with qualifications and experience. A comprehensive benefits package is included with this position. The position is available immediately for an initial period of 1 year, and will be extended subject to satisfactory performance. Interested candidates should send a cover letter outlining research experience and interests, a curriculum vitae, and the names and phone numbers of 3 referees to Ljerka Kunst, Department of Botany, University of British Columbia, 6270 University Blvd., Vancouver, B.C. V6T 1Z4, Canada; FAX (604) 822-6089; E-mail kunst@interchange.ubc.ca. Ljerka Kunst Assistant Professor Department of Botany University of British Columbia 6270 University Blvd. Vancouver, B.C. V6T 1Z4 Phone: (604) 822-2351 FAX: (604) 822-6089 E-mail kunst@interchange.ubc.ca http://www.botany.ubc.ca/Kunst/index.htm --- From owner-arab-gen@hgmp.mrc.ac.uk Tue Mar 7 01:39:08 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id E2FFD17ABA; Tue, 7 Mar 2000 01:39:00 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 1E11F17AAA; Tue, 7 Mar 2000 01:38:57 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: nraikhel@pilot.msu.edu (Natasha Raikhel) Subject: The Society Name Change Date: 6 Mar 2000 22:04:21 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <4.1.20000306115622.00b526f0@pilot.msu.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Colleagues,

 A few months ago, ASPP created a page called "Comments on the Society Name
 Change" on its website (<http://aspp.org) and invited people to express  their opinions of whether or not the name of American Society of Plant  Physiologists should be changed to American Society of Plant Biologists. A  few people expressed their views, but only a few. I was the one who  suggested this change, so I was asked by the ASPP Executive Committee to  motivate people to express their views.
 
Whether you are currently an ASPP member or not, it would be very nice if  you would find a minute to express your view. I am appealing especially to  young professors, postdocs, and graduate students. A strong and active Society with a name that identifies its activities is essential  for our discipline to be successful a prosper. This is necessary for our  presentations before the U.S. Congress, for publication of our journals, and for
 establishing our (changing) identity. It is my personal opinion that it is very important
 for our Society to involve the community of young scientists who are making  important discoveries in plant science to be active participants of our  Society and its decisions. However, I would also like to emphasize that  this participation is not only necessary for the Society itself, but it is  also crucial for the young generation as well. It is a two-way street.

Just changing the name will not change the substance of the  Society. On the other hand, changing the name and giving the society  broader appeal reflects what we really are today and may attract a larger share of the younger  generation. I urge you to take a minute of your valuable time and express  your views on the ASPP web page. If the majority of people do not think  that it is a good idea to change the name, then it will probably not be
put on the ballot. If  the majority think that this change reflects the reality that the fields
of plant biology that we cover extend much beyond plant physiology, then this  question will be put on the ballot, to be decided by vote of the  membership.

 Sincerely yours,
 Natasha Raikhel


Natasha Raikhel
MSU-DOE Plant Research Laboratory
Michigan State University
East Lansing, MI 48824-1312 USA
Phone: (517) 353-3518
Fax: (517) 353-9168
e-mail: nraikhel@pilot.msu.edu

 From owner-arab-gen@hgmp.mrc.ac.uk Tue Mar 7 17:54:55 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 7DC0217AC7; Tue, 7 Mar 2000 17:54:49 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id C7B8317A79; Tue, 7 Mar 2000 17:54:46 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: rupam@scitec.kobe-u.ac.jp (Abidur rahman) Subject: Gravitropic nature of root Date: 7 Mar 2000 09:12:54 -0000 Organization: Kobe University Message-ID: <38C4C764.7DA20184@scitec.kobe-u.ac.jp> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk I am interested about the gravitropic response of Arabidopsis roots and the involvement of Auxin and ethylene in this process. The Arabidopsis auxin resistant mutants aux1\,axr1,axr2,axr3,axr4&dwf  showed a defect in root gravitropic response. Recently two auxin resistant mutants tir1-1and ask1-1 have been reported (Genes & Development 12:198-207 & 13:1678-4691). Could anybody tell me anything about the gravitropic nature of the root of these two mutants?

Thanks in anticipation.

ABIDUR RAHMAN
GRAD.SCHOOL OF SCIENCE & TECHNOLOGY
KOBE UNIVERSITY
KOBE-JAPAN From owner-arab-gen@hgmp.mrc.ac.uk Tue Mar 7 20:10:08 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id A620317A95; Tue, 7 Mar 2000 20:10:04 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 1141517A6F; Tue, 7 Mar 2000 20:10:02 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: delgadoi@pilot.msu.edu (Ivan Delgado) Subject: Stablishment of Arabidopsis cell suspension cultures Date: 7 Mar 2000 18:11:13 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Arabineters, I am interested in establishing a cell suspension culture from Arabidopsis. We already have one (Axelos M, Curie C, Mazzolini L, Bardet C, Lescure B (1992) A protocol for transient expression in Arabidopsis thaliana protoplasts isolated from cell suspension cultures. Plant Physiol Biochem 30 (1): 123-8), but I am interested in having cell suspensions from transgenic plants. Any help in this regard is very appreciated, Ivan P.S. alternatively, a protocol to transform Arabidopsis cell suspensions would be useful but my experience has been that it is a lot harder to obtain transgenic suspensions that way Ivan J. Delgado Orlic Graduate Student MSU-DOE-Plant Research Laboratory Genetics Department Michigan State University 178 Wilson Rd. 122 Plant Biology Building East Lansing, MI 48824-1312 Email: delgadoi@pilot.msu.edu URL: http://www.msu.edu/~delgadoi/ Phone: 517-353-3519 FAX: 517-353-9168 A good idea will keep you awake during the morning, but a great idea will keep you awake during the night -Marilyn Vos Savant --- From owner-arab-gen@hgmp.mrc.ac.uk Tue Mar 7 20:10:55 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 4E85917AED; Tue, 7 Mar 2000 20:10:47 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 082F217A95; Tue, 7 Mar 2000 20:10:42 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: goldj0@uleth.ca ("Joanne Golden") Subject: SSLP fragment Date: 7 Mar 2000 18:13:41 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <38C546EB.338ABA37@uleth.ca> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk We are mapping using PCR-amplified SSLP markers between Col and LER ecotypes. Our problem is in detecting small differences between small sized fragments (e.g. 114 bp with a difference of only 4 bp). The PCR reactions conditions are standard: genomic DNA, 5pM of each primer, 200uM dNTP"s, 50mM KCl, 10mM Tris-HCl pH 9, 2mM Mg++, 0.01% gelatin, 0.1% Triton X-100 and 2 units of TDP in 20ul reactions. Cycling conditions are 94C for 15 seconds, 55C for 15 seconds, and 72C for 30 seconds X40 cycles. The products have been run out on 8% non-denaturing polyacrylamide gels and 2% SMW agarose in 1XTBE. The bands on the PAGE gel are wavy and very uneven, making reliable fragment size assessment difficult. Could the detergent or other components in the PCR product be affecting the mobility of the fragments, and if so, is there a quick and cheap clean-up protocol for the PCR product? On the agarose gels, the same products show even bands, but resolution of small size differences is not as clear. We would appreciate any advice on the best protocol for band resolution. --- From owner-arab-gen@hgmp.mrc.ac.uk Tue Mar 7 20:11:31 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 3DE0B17AFD; Tue, 7 Mar 2000 20:11:27 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id C049917AED; Tue, 7 Mar 2000 20:11:25 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: nraikhel@pilot.msu.edu (Natasha Raikhel) Subject: Society Name Change #2 Date: 7 Mar 2000 18:58:31 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <4.1.20000307085121.00b16880@pilot.msu.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Colleagues,

This morning I was overwhelmed with responses to my message about the ASPP name change. It is great that people do have and do express their views. Many of you said that the name change is long overdue: push, Natasha, this is the right thing to do! But I cannot do more than I have done. If we want this to happen, you must post your opinion on the ASPP website. The messages that have been sent to me are nice; I see that I am not alone. However, we cannot make a difference this way. You don't have to write a long message to ASPP, a very short one will suffice.

You can send your message directly via email; the address is   namechange@aspp.org
It will be posted on the website.

Or you can get to the website, by typing (http://www.aspp.org), then selecting "Hot News." On that screen you will immediately see that one of the "Hot topics" is Comments on the Society Name Change. Clicking on this will take you to the page where you can read other's responses and send your own to ASPP. Simply click the e-mail address (namechange@aspp.org) and write your comments.

Thank you for your response!
Best wishes,
Natasha Raikhel

Natasha Raikhel
MSU-DOE Plant Research Laboratory
Michigan State University
East Lansing, MI 48824-1312 USA
Phone: (517) 353-3518
Fax: (517) 353-9168
e-mail: nraikhel@pilot.msu.edu

 From owner-arab-gen@hgmp.mrc.ac.uk Tue Mar 7 23:14:11 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id DA04017B0D; Tue, 7 Mar 2000 23:14:06 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 91C2917B0C; Tue, 7 Mar 2000 23:14:05 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: rhee@acoma.Stanford.EDU (Sue Rhee) Subject: Updated Seq Map/Table at TAIR Date: 7 Mar 2000 21:22:35 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Arabidopsis researchers, Updated Sequence maps and tables now include orientation of most of Kazusa's sequences relative to the chromsome on chr III and chr V. This was done by blasting their untrimmed sequences, kindly provided by Drs. Yasukazu Nakamura and Satoshi Tabata at the Kazusa DNA Research Institute, against one or both of their neighboring sequences. The untrimmed sequences are available from Kazusa's and our ftp sites. Seq Map: http://arabidopsis.org/cgi-bin/maps/Schrom Seq Table: http://arabidopsis.org/cgi-bin/maps/Seqtable.pl Kazusa's FTP: ftp://ftp.kazusa.or.jp/pub/arabidopsis/bulk/ TAIR's FTP: ftp://tairpub:tairpub@ftp.arabidopsis.org/home/tair/AGI/no_trim/ The TAIR Team, curator@arabidopsis.org --- From owner-arab-gen@hgmp.mrc.ac.uk Tue Mar 7 23:14:42 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 83F7217B15; Tue, 7 Mar 2000 23:14:34 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 8F87F17B0D; Tue, 7 Mar 2000 23:14:30 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: skd4@psu.edu (Sendil Devadas) Subject: Large insert cloning Date: 7 Mar 2000 23:04:19 -0000 Organization: Penn State Message-ID: <38C5457C.8C7F1C6A@psu.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear folks, I am having trouble cloning a 100 kb BAC insert into a TAC vector (22 kb) for eventual complementation. Any suggestion regarding DNA isolation ..digestion..ligation and transformation will be very helpful for me. I supplement my digestion with spermidine, BSA, DTT and do a B-agarase treatment of my low melting CHEF gel and drop dialyse my ligation mix. My vector is CIP treated and I use 10:1 (vector:insert) for ligation, supplemented with ATP. I follow the Gibco-BRl electroporator protocol for transforming DH10b cells. Advance thanks for your help Regards Sendil Devadas Graduate Student Program in Plant Physiology Penn State University PA 16802 --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Mar 8 16:56:52 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 520DF17AE2; Wed, 8 Mar 2000 16:56:46 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 357B817AAD; Wed, 8 Mar 2000 16:56:43 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: "steve.jobling@mercury ("@hgmp.mrc.ac.uk, steve.jobling@hgmp.mrc.ac.uk, " "@Unilever.com Subject: transfer of DNa outside T-DNA borders Date: 8 Mar 2000 12:23:56 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <38C6462D.72CA6E91@Unilever.com> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk This is my first posting to the bulletin board and I thought this question would be a good test! Can anyone give me some info on what is the frequency of transfer of DNA outside of the T-DNA borders and what is the most importnat factor that influences this (transformation vector, crop species or Agrobacterium strain). Steve Dr Steve Jobling Plant Science Unilever Research Colworth Colworth House Sharnbrook Bedford MK44 1LQ UK Tel 44 1234 222575 Fax 44 1234 248010 or 222552 Email Steve.Jobling@Unilever.com --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Mar 8 16:57:02 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id BB8D917AAD; Wed, 8 Mar 2000 16:56:47 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id CF1E817AD9; Wed, 8 Mar 2000 16:56:44 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: naito@abs.agr.hokudai.ac.jp (Satoshi Naito) Subject: mRNA stability/control mRNA Date: 8 Mar 2000 11:38:28 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <200003081137.UAA06107@orange.agr.hokudai.ac.jp> References: <38C5457C.8C7F1C6A@psu.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Folks, To analyze mRNA decay by Northern after actinomycin D etc treatment, we are looking for a control mRNA. Does anyone know an Arabidopsis mRNA which has a half-life of several hours and is abundantly present (ie, easy to detect) ? Thank you. Satoshi Naito =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Satoshi Naito Division of Applied Bioscience Graduate School of Agriculture, Hokkaido University Sapporo 060-8589, JAPAN Phone: +81-11-706-2800 FAX: +81-11-706-4932 e-mail: naito@abs.agr.hokudai.ac.jp http://arabi4.agr.hokudai.ac.jp/ArabiE/arabie.html =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Mar 8 16:57:04 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 3033D17AE4; Wed, 8 Mar 2000 16:56:55 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 72F5D17AD4; Wed, 8 Mar 2000 16:56:43 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: jlb@ncgr.org ("Jeff L. Blanchard") Subject: Bioinformatics related courses Date: 8 Mar 2000 16:22:55 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <200003081622.JAA12101@gallina.ncgr.org> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk This summer the BioPharmaceutical Technology Center Institute in Madison, WI will be offering the following two bioinformatics-related courses immediately before and after the Arabidopsis meeting. For more information see http://www.btci.org/courses/CourseList/courses.htm or contact Jeff Blanchard at the address below. ---------------------------------------------------------------------- ---------- Techniques in Bioinformatics and Comparative Genomics June 18 - June 23, 2000 Objectives and Goals: This five-day intensive computer laboratory course is designed to help participants construct a working library of bioinformatic tools and resources. The format will combine lectures and interactive problem-solving sessions with each participant working individually at a computer. An emphasis will be placed on analysis of practical problems posed by the participants. The flow of the course will move from traditional sequence analysis techniques to the opportunities afforded by the imminent flood of genomic information. Afternoon research seminars by the instructors will sample academic and private sector visions of bioinformatics and highlight creative approaches to utilizing genomic data. (Please note: this course is designed to provide an overview of the field; detailed training in specific packages will not be provided.) Instructors: Jeff Blanchard, Ph.D., Research Scientist, National Center for Genome Resources Tim Burland, Ph.D., Vice President and General Manager, DNASTAR Barbara Butler, Ph.D., Bioinformatics Training and Education Group Leader, Genetics Computer Group Ross Overbeek, Ph.D., Senior Computer Scientist, Integrated Genomics Ann Palmenberg, Ph.D., Professor, Institute for Molecular Virology Department of Biochemistry,University of Wisconsin Michael Slater, Ph.D., Senior Scientist, Promega Corporation Jeff Thorne, Ph.D., Assistant Professor, Department of Statistics, North Carolina State University Jennifer Weller, Ph.D., Senior Scientist, National Center for Genome Resources ---------------------------------------------------------------------- Database Design and Development for Genomics Research June 29 - July 1, 2000 Course Overview: Databases have been the quiet intermediary of molecular biology research and in their current state offer a wonderful example of using the web to share experimental results and knowledge. In recent years there has been a proliferation of individual and community oriented databases that contain DNA sequence, expression, structural, enzymatic, phenotypic, organismal and other types of data. Many scientists and private companies now face the challenge of integrating their data into these heterogeneous databases and/or synthesizing databases before they can finally get to the heart of a research question. This three-day "hands on" workshop will start with a session on "What are databases?" and move onto to database issues in molecular biology related to storing, integrating, visualizing, analyzing, synthesizing and presenting data. The workshop is geared for people in molecular biology lab groups and computer scientists getting into bioinformatics. The format will combine discussions with problem-solving sessions and each participant will work individually at a computer. Instructors: Jeff Blanchard, Ph.D., Research Scientist, National Center for Genome Resources Carol Bult, Ph.D., Research Scientist, Mouse Genome Informatics Group, The Jackson Laboratory Elizabeth Shoop, Ph.D., Research Associate, Computational Biology Center, University of Minnesota Lincoln Stein, Ph.D., Assistant Professor, Cold Spring Harbor Laboratory Matteo di Tommaso, Ph.D., Manager, Database Product Development, Genetics Computer Group ---------------------------------------------------------------------- The BioPharmaceutical Technology Center Institute (BTCI) is a not-for-profit organization operated exclusively for educational, scientific and cultural purposes. One goal of BTCI is to promote the exchange of scientific, educational and cultural information between industry, educators and the general public by providing facilities and resources to support conferences, seminars, classes, and electronic distribution of programs. _____________________________________ Jeffrey L. Blanchard Research Scientist National Center for Genome Resources 2935 Rodeo Park Drive East Santa Fe, NM 87505 tel: 505-995-4405 fax: 505-995-4432 http://www.ncgr.org/about/staff/jlb --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Mar 8 21:07:14 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 5A78A17B2D; Wed, 8 Mar 2000 21:07:11 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 461D917B14; Wed, 8 Mar 2000 21:07:10 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: smitam01@holmes.ipfw.edu (Alan Smith) Subject: Inexpensive X-Gluc Date: 8 Mar 2000 18:31:02 -0000 Organization: Indiana University Purdue University Ft. Wayne Message-ID: <38C69C59.B499F22A@holmes.ipfw.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hello Everyone, I am having a very difficult time finding companies which specialize in selling the GUS substrate X-GlucA (5-Bromo-4-chloro-3-indoyl-3-D-Glucuronic acid) at inexpensive prices. I was able to find X-Gluc at the large chemical companies (Sigma-Aldrich), but it was extremely expensive. We only need a small quantity of X-Gluc, about 200mg. Any information about companies which sell X-GlucA at a decent price would be greatly appreciated. Thank You Alan Smith Graduate Student Dept. of Biology Indiana-Purdue University email: smitam01@holmes.ipfw.edu phone: 219-481-5434 --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Mar 8 21:07:32 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id A3FDB17B30; Wed, 8 Mar 2000 21:07:29 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id B4F1117B2F; Wed, 8 Mar 2000 21:07:28 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: jlb@ncgr.org ("Jeff L. Blanchard") Subject: Bioinformatics related courses - correction Date: 8 Mar 2000 19:02:29 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <200003081901.MAA12432@gallina.ncgr.org> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk The following message includes the correct URL for the BTCI courses. This summer the BioPharmaceutical Technology Center Institute in Madison, WI will be offering the following two bioinformatics-related courses. For more information see http://www.btci.org/courses/CoursesList/courses.htm or contact Jeff Blanchard at the address below. ---------------------------------------------------------------------- ---------- Techniques in Bioinformatics and Comparative Genomics June 18 - June 23, 2000 Objectives and Goals: This five-day intensive computer laboratory course is designed to help participants construct a working library of bioinformatic tools and resources. The format will combine lectures and interactive problem-solving sessions with each participant working individually at a computer. An emphasis will be placed on analysis of practical problems posed by the participants. The flow of the course will move from traditional sequence analysis techniques to the opportunities afforded by the imminent flood of genomic information. Afternoon research seminars by the instructors will sample academic and private sector visions of bioinformatics and highlight creative approaches to utilizing genomic data. (Please note: this course is designed to provide an overview of the field; detailed training in specific packages will not be provided.) Instructors: Jeff Blanchard, Ph.D., Research Scientist, National Center for Genome Resources Tim Burland, Ph.D., Vice President and General Manager, DNASTAR Barbara Butler, Ph.D., Bioinformatics Training and Education Group Leader, Genetics Computer Group Ross Overbeek, Ph.D., Senior Computer Scientist, Integrated Genomics Ann Palmenberg, Ph.D., Professor, Institute for Molecular Virology Department of Biochemistry,University of Wisconsin Michael Slater, Ph.D., Senior Scientist, Promega Corporation Jeff Thorne, Ph.D., Assistant Professor, Department of Statistics, North Carolina State University Jennifer Weller, Ph.D., Senior Scientist, National Center for Genome Resources ---------------------------------------------------------------------- Database Design and Development for Genomics Research June 29 - July 1, 2000 Course Overview: Databases have been the quiet intermediary of molecular biology research and in their current state offer a wonderful example of using the web to share experimental results and knowledge. In recent years there has been a proliferation of individual and community oriented databases that contain DNA sequence, expression, structural, enzymatic, phenotypic, organismal and other types of data. Many scientists and private companies now face the challenge of integrating their data into these heterogeneous databases and/or synthesizing databases before they can finally get to the heart of a research question. This three-day "hands on" workshop will start with a session on "What are databases?" and move onto to database issues in molecular biology related to storing, integrating, visualizing, analyzing, synthesizing and presenting data. The workshop is geared for people in molecular biology lab groups and computer scientists getting into bioinformatics. The format will combine discussions with problem-solving sessions and each participant will work individually at a computer. Instructors: Jeff Blanchard, Ph.D., Research Scientist, National Center for Genome Resources Carol Bult, Ph.D., Research Scientist, Mouse Genome Informatics Group, The Jackson Laboratory Elizabeth Shoop, Ph.D., Research Associate, Computational Biology Center, University of Minnesota Lincoln Stein, Ph.D., Assistant Professor, Cold Spring Harbor Laboratory Matteo di Tommaso, Ph.D., Manager, Database Product Development, Genetics Computer Group ---------------------------------------------------------------------- The BioPharmaceutical Technology Center Institute (BTCI) is a not-for-profit organization operated exclusively for educational, scientific and cultural purposes. One goal of BTCI is to promote the exchange of scientific, educational and cultural information between industry, educators and the general public by providing facilities and resources to support conferences, seminars, classes, and electronic distribution of programs. _____________________________________ Jeffrey L. Blanchard Research Scientist National Center for Genome Resources 2935 Rodeo Park Drive East Santa Fe, NM 87505 tel: 505-995-4405 fax: 505-995-4432 http://www.ncgr.org/about/staff/jlb --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Mar 9 02:26:53 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 3A0AC17A98; Thu, 9 Mar 2000 02:26:50 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id DA77017A75; Thu, 9 Mar 2000 02:26:48 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: BaskinT@missouri.edu (Tobias Baskin) Subject: GUS reaction chemistry Date: 8 Mar 2000 21:48:52 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: References: <38C69C59.B499F22A@holmes.ipfw.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Greetings, I am looking for information about the chemical reactions leading to our familiar blue precipitate in the presence of the GUS enzyme. In particular, I'd like to learn more about what the iron salts are doing. Although I have wondered about this for some time, it became more pressing the other day when we observed a TOTALLY different staining pattern when the iron salts were replaced by EDTA in the buffer. If someone could explain this or point me to a good reference on this topic, I'd be very grateful. (I did look through the recent "GUS protocols" book but it presented lots of protocols but I couldn't find anything helpful about the chemistry, at least not from the table of contents.) Thanks in advance, Tobias Baskin _ ____ ^ __ ____ Tobias I. Baskin / \ / / \ / \ \ University of Missouri / | / / \ \ \ Biological Sciences /___/ /__ /___ \ \ \__ 109 Tucker Hall / / / \ \ \ Columbia, MO 65211-7400 USA / / / \ \ \ voice: 573-882-0173 / /____ / \ \__/ \____ fax: 573-882-0123 --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Mar 9 16:02:17 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 93C9317A87; Thu, 9 Mar 2000 16:02:11 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id D8B0C17A67; Thu, 9 Mar 2000 16:02:07 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: Nancy Terryn Subject: Re: Chromosome 5 Date: Thu, 09 Mar 2000 10:52:36 +0100 Organization: University of Ghent, Belgium Message-ID: <38C77464.70FE074@gengenp.rug.ac.be> References: <38C17081.321172E5@calstatela.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Janel, info on chromosome 5 can be found both at the European MIPS site : http://www.mips.biochem.mpg.de/proj/thal/chr5_index.html and the Japanese Kazusa site : http://www.kazusa.or.jp/kaos/cgi-bin/agd_chrlist.cgi?c=5 Good luck! Nancy -- ================================================================== Nancy Terryn DEPARTMENT OF GENETICS Fax:32 (0)9 2645349 UNIVERSITY OF GENT, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium Vlaams Instituut voor Biotechnologie VIB mailto:nater@gengenp.rug.ac.be http://www.plantgenetics.rug.ac.be ================================================================== From owner-arab-gen@hgmp.mrc.ac.uk Thu Mar 9 21:35:46 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id E511317AFA; Thu, 9 Mar 2000 21:35:42 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 9C90017A90; Thu, 9 Mar 2000 21:35:40 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: sean_may@thale.life.nottingham.ac.uk ("Sean May [NASC]") Subject: Release of GAL4-GFP 'M' lines from Jim Haseloff Date: 9 Mar 2000 20:07:56 -0000 Organization: NASC Message-ID: <8a90a3$sbp$1@oyez.ccc.nottingham.ac.uk> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Release of GAL4-GFP lines from Jim Haseloff NASC and ABRC are pleased to announce the release of several additional GAL4-GFP Enhancer-trap Arabidopsis lines from Dr. Jim Haseloff, MRC Laboratory of Molecular Biology, Cambridge, UK. Set 'M' (new release - 70 lines) and set 'KS' (already available - 31 lines) have been pre screened for shoot and floral expression. They show a variety of general and specific patterns of fluorescence in organs, tissues and cells ranging from the funiculus to the shoot apex. Several of these lines also show general and specific reporter expression in root tissue. Set 'M' is stock number CS/N9342. For detailed descriptions of these lines, and information relating to these lines, please refer to Jim Haseloff's web page: http://www.plantsci.cam.ac.uk/Haseloff/Home.html Stocks can be ordered in the usual way from the NASC (http://nasc.nott.ac.uk) and ABRC (http://aims.cse.msu.edu/aims) web sites. The handling charges associated with these lines are: CS/N9342 (M series) NASC 64 pounds sterling ABRC 100 US dollars Sean May (NASC) Randy Scholl (ABRC) Sean_May@nasc.nott.ac.uk Director, Nottingham Arabidopsis Stock Centre (NASC) University Park, Nottingham University. U.K. NG7 2RD. FON: +44 (0)115 9513237 FAX: +44 (0)115 9513297 Catalogue: http://nasc.nott.ac.uk UKCropNet: http://synteny.nott.ac.uk Dr. Randall L. Scholl ( scholl.1@osu.edu ) Associate Professor, Plant Biology Room 363 B&Z Bldg. 1735 Neil Avenue, Columbus, OH 43210 Office: (614) 292-1982 Lab: (614) 292-9371 Fax: (614)-292-0603 http://aims.cps.msu.edu/aims/ --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Mar 10 17:23:23 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id ED69817AF2; Fri, 10 Mar 2000 17:23:17 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 2AE6317AE0; Fri, 10 Mar 2000 17:23:14 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: rhee@acoma.Stanford.EDU (Sue Rhee) Subject: BLAST service at TAIR Date: 10 Mar 2000 02:51:57 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Arabidopsis researchers, Thank you for all your input regarding TAIR BLAST. We have changed a few things to hopefully improve the performance (using more than one server, preventing browser timeout, etc...) In order to improve the performance for everyone, we would like to impose the following limits on the input sequence size starting tomorrow, Friday, March 10th. BLASTN = 25000 bp (All datasets) BLASTX = 25000 bp (All datasets) BLASTP = 5000 aa (All datasets) TBLASTN = 1000 aa (Large datasets. ArabidopsisN, etc) TBLASTN = 5000 aa (Small datasets, New GenBank and similar size datasets) TBLASTX = 1000 bp (Large datasets. ArabidopsisN, etc) TBLASTX = 5000 bp (Small datasets, New GenBank and similar size datasets) Also you will be able to query upto 10 sequences at once (in FastA format), given that the total size doesn't exceed the above limits. ===================================================== This could be summarized as: nucleotide queries - 25 kb except tblastx/large datasets (1 kb) and tblastx/small datasets (5 kb) amino acid queries - 5000 aa except tblastn/large datasets (1000 aa) Large datasets = GenBankPlus, GenBankMinus, Protein, GenBank >10K, All Plant Please let us know if you have any problems with these limits. Thanks, The TAIR Team www.arabidopsis.org curator@arabidopsis.org --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Mar 10 17:24:09 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id B995317AF7; Fri, 10 Mar 2000 17:23:57 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id AB4D917AF2; Fri, 10 Mar 2000 17:23:50 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: h.fromm@leeds.ac.uk ("H.Fromm") Subject: GM crops and abiotic stress tolerance Date: 10 Mar 2000 09:53:25 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <200003100953.JAA15354@gps.leeds.ac.uk> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear All, I wonder if anyone has good examples of GM crops engineered for abiotic stress tolerance (e.g., high salinity, cold, heat, etc.), which successfully passed the stage of field trials. Thanks. Dr. H. Fromm Centre for Plant Sciences Leeds Institute for Biotechnology and Agriculture (LIBA) University of Leeds LS2 9JT, UK E-mail: h.fromm@leeds.ac.uk --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Mar 10 17:24:33 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 104EA17AF7; Fri, 10 Mar 2000 17:24:21 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id D34B917B59; Fri, 10 Mar 2000 17:24:17 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: siddiqui@scar.utoronto.ca (Najeeb Siddiqui) Subject: Re: Inexpensive X-Gluc Date: 10 Mar 2000 14:31:46 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <200003101431.JAA10690@banks.scar> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Research Products International offers great deals on X-Gluc. Check www.rpicorp.com Najeeb Siddiqui Dept. of Botany University of Toronto --- From owner-arab-gen@hgmp.mrc.ac.uk Sat Mar 11 03:29:21 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 335C517AFB; Sat, 11 Mar 2000 03:29:17 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 33F6D17A6A; Sat, 11 Mar 2000 03:29:14 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: maysong@ucla.edu ("M. Santiago Ong") Subject: biliverdin Date: 11 Mar 2000 02:16:18 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Biliverdin is no longer carried by Sigma. Can anyone recommend a vendor? Thanks, May Santiago Ong Graduate Student UCLA --- From owner-arab-gen@hgmp.mrc.ac.uk Sat Mar 11 21:27:06 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 75BBD17AA2; Sat, 11 Mar 2000 21:27:02 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 33B4D17A59; Sat, 11 Mar 2000 21:26:59 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: curator@arabidopsis.org Subject: New/Updated Arabidopsis DNA 11-Mar-2000 Date: 11 Mar 2000 14:16:15 -0000 Organization: The Arabidopsis Information Resource Message-ID: <200003111415.HAA26580@cleveland.ncgr.org> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Arabidopsis researchers, The following information is the number of Arabidopsis entries in GenBank that are new or were updated in the last week. These sequences have been updated in TAIR and are available for searching using TAIR BLAST and FASTA at: http://arabidopsis.org/blast/ http://arabidopsis.org/cgi-bin/fasta/TAIRfasta.pl New sequences : 9 Updated sequences : 0 Updated annotation : 4 ====================== New DNA Sequences ======================= AB034696 AB034696 2991bp mRNA PLN 23-FEB-2000 Arabidopsis thaliana AVPL1 mRNA for vacuolar-pyrophosphatase like protein, complete cds. vacuolar-pyrophosphatase like protein; AVPL1. AF145451 AF145451 1579bp mRNA PLN 23-FEB-2000 Arabidopsis thaliana branched chain alpha-keto acid dehydrogenase E2 subunit (din3) mRNA, complete cds; nuclear gene for mitochondrial product. din3; branched chain alpha-keto acid dehydrogenase E2 subunit. AF145452 AF145452 1326bp mRNA PLN 23-FEB-2000 Arabidopsis thaliana branched chain alpha-keto acid dehydrogenase E1 beta subunit (din4) mRNA, complete cds; nuclear gene for mitochondrial product. din4; branched chain alpha-keto acid dehydrogenase E1 beta subunit. AF225913 AF225913 1178bp mRNA PLN 23-FEB-2000 Arabidopsis thaliana putative cytochrome c biogenesis protein mRNA, complete cds. putative cytochrome c biogenesis protein precursor. AF225966 AF225966 709bp DNA PLN 23-FEB-2000 Arabidopsis thaliana peroxidase ATP A2 gene, promoter region. peroxidase ATP A2. AF059510 AF059510 1907bp mRNA PLN 23-FEB-2000 Arabidopsis thaliana 3-methylcrotonyl-CoA carboxylase non-biotinylated subunit (MCCB) mRNA, complete cds. MCCB; 3-methylcrotonyl-CoA carboxylase non-biotinylated subunit. AF059511 AF059511 5280bp DNA PLN 23-FEB-2000 Arabidopsis thaliana 3-methylcrotonyl-CoA carboxylase nonbiotinylated subunit (MCCB) gene, complete cds. 3-methylcrotonyl-CoA carboxylase nonbiotinylated subunit; MCCB. AF056971 AF056971 2944bp DNA PLN 23-FEB-2000 Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase (RBCL) gene, partial cds; and carboxyltransferase beta subunit (ACCD) gene, complete cds; chloroplast genes for chloroplast products. ribulose-1,5-bisphosphate carboxylase; RBCL; carboxyltransferase beta subunit; ACCD. AC024081 AC024081 82893bp DNA PLN 23-FEB-2000 Arabidopsis thaliana chromosome III P1 MJK13 genomic sequence, complete sequence. HTG; MJK13.1; putative ubiquitin-conjugating enzyme, 5' partial; MJK13.2; thioredoxin m4; MJK13.3; putative expansin S2 precursor; MJK13.4; MJK13.5; MJK13.6; anther development protein, ATA20; MJK13.7; MJK13.8; MJK13.9; MJK13.10; MJK13.11; MJK13.12; MJK13.13; MJK13.14; MJK13.15; MJK13.16; putative jasmonic acid regulatory protein; MJK13.17; MJK13.18; MJK13.19; MJK13.20; early auxin-induced protein, IAA19; MJK13.21. =========== Updated Sequence Features/Annotations ============= ATH010713 AJ010713 2782bp DNA PLN 02-MAR-2000 Arabidopsis thaliana fdh gene. fdh gene; fiddlehead protein; fdh. ATU90264 U90264 1181bp DNA PLN 23-FEB-2000 Arabidopsis thaliana bifunctional nuclease bfn1 (bfn1) gene, complete cds. bifunctional nuclease bfn1; bfn1. AC015447 AC015447 108370bp DNA HTG 23-FEB-2000 Arabidopsis thaliana chromosome I clone F24J8, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC020580 AC020580 95751bp DNA HTG 23-FEB-2000 Arabidopsis thaliana chromosome III clone IGF-F5E6, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. --- From owner-arab-gen@hgmp.mrc.ac.uk Sat Mar 11 21:27:52 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 9804917AA3; Sat, 11 Mar 2000 21:27:48 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 3B18117AA2; Sat, 11 Mar 2000 21:27:47 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: smitam01@holmes.ipfw.edu (Alan Smith) Subject: Summary Inexpensive X-Gluc Date: 11 Mar 2000 18:39:25 -0000 Organization: Indiana University Purdue University Ft. Wayne Message-ID: <38CA92BD.A7BA9128@holmes.ipfw.edu> References: <38C69C59.B499F22A@holmes.ipfw.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hello Everyone, This is a summary of emails I recieved from my earlier post to the newsgroup about X-Gluc prices. I am assuming all of these companies sell X-Gluc at a discounted price. --AaeChem Inc. of Bloomington IN have decent prices (MUCH CHEAPER THAN SIGMA). Their phone # is (812)334-9996 --Gold Biotechnology Inc, in St Louis, An empty bottle has this phone number on it:1-800-248-7609. --Calbiochem. I think we paid around $30-$40 for 100mg. --Denville Scientific Inc. (1-800-453-0385) carries X-Gluc. The price is about 65 dollar for 100mg. --Biosynth AG in Switzerland. http://www.biosynth.com/ --Melford Laboratories Ltd. Chelsworth, Ipswich, Suffolk, IP7 7LE. Tel: 01449 741178. 1g costs £245 --Rose Scientific of Edmonton, Alberta. 1 gram amounts for about 25% of other vendors. Phone: 1-800-661-9288. FAX: 403-462-5776 --San Diego company called DocFrugal for $69.90/100mg. --Research Products International www.rpicorp.com Great thanks to all who replied Alan Smith Graduate Student Dept of Biology Indiana-Purdue University Phone: 219-481-5434 email: smitam01@holmes.ipfw.edu Alan Smith wrote: > Hello Everyone, > > I am having a very difficult time finding companies which specialize in > selling the GUS substrate X-GlucA > (5-Bromo-4-chloro-3-indoyl-3-D-Glucuronic acid) at inexpensive prices. > I was able to find X-Gluc at the large chemical companies > (Sigma-Aldrich), but it was extremely expensive. We only need a small > quantity of X-Gluc, about 200mg. Any information about companies which > sell X-GlucA at a decent price would be greatly appreciated. > > Thank You > Alan Smith > Graduate Student > Dept. of Biology > Indiana-Purdue University > email: smitam01@holmes.ipfw.edu > phone: 219-481-5434 > > --- --- From owner-arab-gen@hgmp.mrc.ac.uk Mon Mar 13 15:55:40 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id DC42117ACD; Mon, 13 Mar 2000 15:55:28 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 90A0717AB5; Mon, 13 Mar 2000 15:55:23 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: Gene.Nomenclature2@bbsrc.ac.uk Subject: Bioinformatisist/Research Scientist Post Date: 13 Mar 2000 10:24:17 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <38CCC020.2BFF6349@bbsrc.ac.uk> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk VACANCY NOTICE 02/381

JOHN INNES CENTRE

JOB TITLE: BIOINFORMATISIST/RESEARCH SCIENTIST
VACANCY NO: 35 08 05
LOCATION: JIC, Norwich
BAND: 6
DATE OF ISSUE: 10 03 00
CLOSING DATE: 23 03 00

PURPOSE AND OBJECTIVES:

A Bioinformatisist is required to undertake detailed analysis and annotation of Arabidopsis genes and their cognate ESTs. The identification of primer sequences for developing gene specific sequence tags will also be undertaken. The project is part of a collaborative effort within the Arabidopsis Functional Genomics Resource Network to develop micro-arrays for the plant science community.

PERSON SPECIFICATION:

The candidate should have a BSc and a minimum of three years experience in a relevant scientific subject together with experience in bioinformatics, a PhD would be desirable. Experience of sequence analysis and familiarity of UNIX, PERL and EXCEL would also be desirable.
 
APPLICATION FORMS AND FURTHER PARTICULARS:

Personnel Dept, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH

or

E-Mail:   JICPERS @ BBSRC.AC.UK
DAVID.LONSDALE@BBSRC.AC.UK

Please quote reference no : 35 08 05 on all correspondence
APPOINTMENT DETAILS:

STATUS:         & nbsp;  Temporary - 3 YEAR POST
DESIGNATION: Fixed
WORK PATTERN:    Full time
SALARY:  £17,300 - £20,000 pa Depending on qualifications and experience

EQUAL OPPORTUNITIES EMPLOYER

******************************
  From owner-arab-gen@hgmp.mrc.ac.uk Mon Mar 13 21:06:13 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 5DD1417A83; Mon, 13 Mar 2000 21:06:10 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 637D517A81; Mon, 13 Mar 2000 21:06:09 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: johnmcd@vt.edu ("Dr. John M. McDowell") Subject: Graduate Assistantships in Plant Molecular Sciences Date: 13 Mar 2000 20:52:29 -0000 Organization: Virginia Tech Message-ID: <38CEA634.6BC8894C@vt.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Graduate Assistantships in Plant Molecular Sciences Virginia Tech, Blacksburg Research/teaching assistantships are available for studies leading to an M.S. or Ph.D. degree. The faculty listed below are using contemporary biochemical, genetic, and genomics-based approaches to address basic and applied problems in the plant sciences: Glenda Gillaspy-signal transduction; John Jelesko-meiotic recombination; John McDowell-plant/pathogen interactions; Jim Westwood-plant/plant interactions. Virginia Tech is among the top 50 US research universities in terms of federal funding, and is rapidly expanding in the areas of biological sciences and biotechnology. Blacksburg is located in an attractive mountain setting and contains all of the standard college town amenities as well as exceptional outdoor recreation opportunities (http://www.bev.net/). Interested individuals should contact John McDowell, johnmcd@vt.edu, 540-231-2388. --- From owner-arab-gen@hgmp.mrc.ac.uk Mon Mar 13 23:43:00 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id EAF7F17ADA; Mon, 13 Mar 2000 23:42:57 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id DDB9817AB6; Mon, 13 Mar 2000 23:42:56 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: weigel@salk.edu (Detlef Weigel) Subject: New NAASC members Date: 13 Mar 2000 23:31:07 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Two new members of the North American Arabidopsis Steering Committee (NAASC) have been elected by electronic ballot. Mike Sussman and Peter McCourt will replace Jeff Dangl and myself. Steve Kay and Chuck Gasser are the new co-chairs. The 2000 NAASC members are: Steve Kay ('98-'00) Chuck Gasser ('98-'00) Kathy Barton ('99-'01) Mary Lou Guerinot ('99-'01) Mike Sussman ('00-'02) Peter McCourt ('00-'02) Thanks to everbody who voted, and thanks to all the nominees for standing for election. --- From owner-arab-gen@hgmp.mrc.ac.uk Tue Mar 14 02:09:55 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 68F4717ADA; Tue, 14 Mar 2000 02:09:52 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 2010517A7F; Tue, 14 Mar 2000 02:09:50 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: joeverica@yahoo.com (Joseph Verica) Subject: promoter lengths Date: 14 Mar 2000 00:05:07 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <20000314000501.6357.qmail@web702.mail.yahoo.com> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk I am looking for information regarding promoter lengths in plant genes. Specifically, what is the length of a "typical" promoter? How much variation in length is there? What is the shortest promoter described? If anyone could provide me with these answers, or better yet a reference, I would appreciate it. Thanks in advance, Joe Verica San Francisco State University __________________________________________________ Do You Yahoo!? Talk to your friends online with Yahoo! Messenger. http://im.yahoo.com --- From owner-arab-gen@hgmp.mrc.ac.uk Tue Mar 14 16:50:23 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 8187C17A9C; Tue, 14 Mar 2000 16:50:19 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id BB02317A9B; Tue, 14 Mar 2000 16:50:17 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: A.Plume@botany.uq.edu.au (Andrew Plume) Subject: Bacillus thuringiensis var. israelensis for fungus gnat control Date: 14 Mar 2000 05:35:09 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <38CDCF57.5D56E3F2@botany.uq.edu.au> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hi everyone, We have just switched the potting mix we use to grow Arabidopsis to one rich in organic matter and have since developed a serious fungus gnat outbreak in our growth room. We have heard about Gnatrol, a commercially available preparation of bacteria (Bacillus thuringiensis var. israelensis) which is used as a soil drench to control the larvae. Has anyone out there ever tried to grow cultures of this strain for themselves to this purpose? Does anyone out there (particularly in Australia, but anywhere will do) have a culture of this strain they are willing to share, and advice on appropraite growth conditions? Cheers, Andrew Plume Plant Genetic Engineering Laboratory The uni of Queensland St Lucia 4067 AUSTRALIA --- From owner-arab-gen@hgmp.mrc.ac.uk Tue Mar 14 16:50:48 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 2CC6817A9D; Tue, 14 Mar 2000 16:50:43 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 121F217A9C; Tue, 14 Mar 2000 16:50:41 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: Jean-David.Rochaix@molbio.unige.ch (Jean-David Rochaix) Subject: position announcement Date: 14 Mar 2000 15:07:38 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <3.0.1.32.20000314155345.00d11c18@sc2a.unige.ch> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk The Faculties of Sciences of the Universities of Geneva and Lausanne, Switzerland, invite applications for two positions of: Professor of Plant Biology Both positions are full-time, beginning October 1st, 2000, and require a Ph.D. or equivalent. Evidence of effective teaching experience and direction of research programs is required. The successful applicant will give courses in French; a grace period can be granted for language acquisition. Both institutions offer excellent research opportunities in multidisciplinary environments. Université de Genève Professeur ordinaire ou adjoint au Département de botanique et biologie végétale Full Professor or Associate Professor in the Department of Plant Biology Candidates will be expected to direct cutting-edge research in the areas of plant molecular and cellular biology. Université de Lausanne Professeur associé à l'Institut d'écologie Associate Professor in the Plant Biology Laboratory of the Institute of Ecology Candidates should have a publication record in plant cell biology. Preference will be given to specialists in structure-function relationships. Applications, including a curriculum vitae, a statement of proposed research, three examples of publications and names and addresses of referees, should be submitted before May 1st 2000 to: For Geneva: Secrétariat de la Faculté des Sciences, Université de Genève, 30 Quai Ernest-Ansermet, CH-1211 Genève 4, Switzerland, where inquiries can be made (Tel. +41-22-702-6652). For further information: E-mail: jean-david.rochaix@molbio.unige.ch. For Lausanne: the Décanat of the Faculty of Science, Collège Propédeutique, CH-1015 Lausanne, Switzerland. For further information on infrastructure, responsablitites and conditions see http://www.unil.ch/ibpv Candidates are encouraged to apply for both positions but if applying for one position must state which one in their applications. The Universities encourage applications from women. Jean-David Rochaix Department of Molecular Biology University of Geneva 30, Quai Ernest Ansermet 1211 Geneva 4 Switzerland Tel +41 22 702 6187 Fax +41 22 702 6868 e-mail Jean-David. Rochaix@molbio.unige.ch --- From owner-arab-gen@hgmp.mrc.ac.uk Tue Mar 14 19:22:36 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 1021217A90; Tue, 14 Mar 2000 19:22:33 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id B8B2417A5D; Tue, 14 Mar 2000 19:22:32 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: buschmann@gsf.de Subject: Chr1 marker Date: 14 Mar 2000 17:23:03 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <3.0.6.32.20000314182135.008ee600@pop> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hello everybody, is anybody familiar with the RFLP marker mi106b? I can not find any precise information about it, only that it has been used to establish BAC contigs and that it localizes to the top of Chr.1. Please note that mi106b is not identical to mi106. mi106b is not included in the mi RFLP-Marker Table at the TAIR web site. Many thanks for any suggestions, Henrik Henrik Buschmann GSF-Research Center for Health and Environment Institute for Biochemical Plant Pathology (BIOP) Oberschleissheim, Germany phone 0049 89 3187 3308 fax 0049 89 3187 2726 --- From owner-arab-gen@hgmp.mrc.ac.uk Tue Mar 14 19:23:03 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id DC7AD17A9E; Tue, 14 Mar 2000 19:22:58 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 79FDD17A90; Tue, 14 Mar 2000 19:22:57 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: peta.bonhamsmith@usask.ca (Peta C. Bonham-Smith) Subject: post-doc on plant ribosome Date: 14 Mar 2000 17:32:07 -0000 Organization: University of Saskatchewan Message-ID: <8alt29$hj1$1@tribune.usask.ca> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk 14th March 2000 POST-DOCTORAL POSITION AVAILABLE TO WORK ON THE PLANT RIBOSOME My lab is interested in determining the protein composition of the plant ribosome, the relatedness of the plant ribosomal proteins (r-proteins) with prokaryotic, yeast & animal r-proteins and the mode of regulation of expression of plant r-proteins. This post-doctoral position will focus on determining the protein composition of the plant ribosome as well as determining the mode of regulation of expression of the 2 r-proteins S6 and S15A. R-protein S6 is a r-protein which can exist in a phosphorylated or unphosphorylated form. Regulation of phosphorylation appears to play a role in regulation of translation by the ribosome. How is expression of r-protein S6 regulated at the transcriptional level? R-protein S15A is a primary binder of 18S rRNA. As such, regulation of it's expression could play a major role in subsequent ribosomal small subunit assembly. How is expression of r-protein S15A regulated at the transcriptional level? The applicant must have a Ph.D. in plant molecular biology or experience in plant molecular biology since graduating. Some experience in protein manipulation would be a bonus. The competition will be open until a suitable candidate has been identified. The position is to start ASAP. Please check my web page at: http://duke.usask.ca/~bonhamp/ for more information concerning my laboratory, its occupants and our research interests. Interested applicants can contact me through email at: bonhamp@duke.usask.ca or via mail at: Dr. Peta Bonham-Smith Department of Biology University of Saskatchewan 112 Science Place Saskatoon S7N 5E2 SK CANADA --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Mar 15 17:10:47 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id D439B17B22; Wed, 15 Mar 2000 17:10:43 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 5401817AA5; Wed, 15 Mar 2000 17:10:40 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: galbraith@arizona.edu (David Galbraith) Subject: Postdoctoral positions available Date: 14 Mar 2000 21:36:41 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <4.2.0.58.20000314143208.00a6ae70@ag.arizona.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk POSTDOCTORAL TRAINING IN THE FUNCTIONAL GENOMICS OF PLANT-INSECT INTERACTIONS. The NSF-supported Research Training Program in Plant-Insect Interactions at the University of Arizona invites applications from prospective postdoctoral fellows. We are particularly interested in identifying candidates to employ functional genomics to analyze the interactions of lepidopteran and homopteran herbivores with host plants. Individual projects include, but are not limited to, studies of gene expression (a) within generalist and specialist insect pests, (b) associated with insect digestive and olfactory processes, and (c) involved with the transmission of phytopathogenic agents. Salary range $25,500-$36,000 based on years of postdoctoral experience. Further information can be obtained from our website at http://ag.arizona.edu/pii-rtg or from Human Resources at http://www.hr.arizona.edu. To apply, send a statement of research accomplishments and aspirations, a résumé, and names of three references to: Plant-Insect Interactions Interdisciplinary Program, Attention: Dr. David Galbraith, 303 Forbes Building, University of Arizona, Tucson, AZ 85721. Please reference job number 17803. Review of applications will begin April 24, 2000 and will continue through December 31, 2000. The University of Arizona is an EEO/AA Employer-M/W/D/V. Please note: Applicants must be citizens or permanent residents of the United States. --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Mar 15 17:11:04 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 2585F17B22; Wed, 15 Mar 2000 17:10:59 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id DC34317B10; Wed, 15 Mar 2000 17:10:57 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: rmtlee@home.com Subject: Tandem Repeat Search Programs Date: 15 Mar 2000 07:22:54 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <38CF3BB2.7C1DCC81@home.com> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hello! I was wondering if there are any programs available on the web for academic use that can search for tandem repeats in sequences that are over 1 Mb in size? Thank you! Robert Lee --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Mar 15 17:12:08 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 95BD217B23; Wed, 15 Mar 2000 17:12:05 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 23DD717B22; Wed, 15 Mar 2000 17:12:03 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: nbaumber@botinst.unizh.ch (Nicolas Baumberger) Subject: SEM of root hairs Date: 15 Mar 2000 12:55:21 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <38CF8615.62316882@botinst.unizh.ch> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear netters, I would like to make scanning electron microscope pictures of Arabidopsis root hairs grown on vertical MS plate. Unfortunately so far I was never able to preserve the fine and delicate structure of the root hairs. The main difficulty is that a loss of humidity of the air during manipulation destroys the hairs in a matter of seconds. We tried to freeze them in liquid nitrogen slush and do the observation in a SEM at low temperature but the short time needed (15-30 seconds) to transfer the root in the freezing device was already too long for most of the root hairs. In addition we had a lot of ice crystals at the surface of the specimen which was difficult to sublime. We also tried to fix them 2 h with OsO4 vapors in a humid chamber followed by dehydration in acetone but this too destroyed the hairs. I am desperatly looking for any advice and trick to make SEM observation of delicate structure like root hairs. Thanks for any answer. -- Nicolas Baumberger Institute of Plant Biology, University of Zürich Zollikerstr.107, CH- 8008 ZURICH, Switzerland Tel: 41-1-6348232 Fax: 41-1-6348257 Email: nbaumber@botinst.unizh.ch --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Mar 15 19:19:37 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 5150417B2C; Wed, 15 Mar 2000 19:19:34 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 14F6D17B26; Wed, 15 Mar 2000 19:19:32 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: schnell@andromeda.rutgers.edu ("Danny Schnell") Subject: Postdoc Position in Chloroplast Protein Import Date: 15 Mar 2000 18:19:20 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <000401bf8eab$93854600$49210680@test.rutgers.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Arabidopsis community, I have two new postdoctoral positions available as of July 2000 to study the mechanism of protein import into chloroplasts and the role of protein import in plastid development. The laboratory will be moving to wonderful new facilities in the Department of Biochemistry and Molecular Biology at the University of Massachusetts, Amherst, MA at the end of the year. Interested individuals could start at Rutgers and move to Amherst or join us in our new home at the end of the year. The official announcement of the positions follows. NIH/NSF funded POSTDOCTORAL POSITIONS are available to investigate the molecular mechanism of protein import into chloroplasts. The projects will involve the characterization of protein import components using combined biochemical and molecular genetic approaches in Arabidopsis. Oualified applicants should have a strong background in biochemistry and molecular biology. Send curriculum vitae with references and cover letter to: Dr. D. Schnell, Rutgers University, Department of Biological Sciences, 101 Warren Street, Newark, NJ 07102. E-mail: schnell@andromeda.rutgers.edu. Webpage: http://biology-newark.rutgers.edu/Schnell/schnell.html Danny J. Schnell, Ph.D. Associate Professor Department of Biological Sciences Rutgers University 101 Warren Street Newark, NJ 07102 Phone: (973) 353-1082 Fax: (973) 353-1007 E-mail: schnell@andromeda.rutgers.edu --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Mar 16 16:47:13 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id DB0B617AAE; Thu, 16 Mar 2000 16:46:57 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 1AD4D17A4E; Thu, 16 Mar 2000 16:46:54 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: schuermanp@missouri.edu ("Peter L. Schuerman") Subject: pOCA28 Date: 15 Mar 2000 21:17:09 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Can anyone tell me where I can find a description of the construction of the pTi-derived cosmid vector pOCA28? Thanks, Peter L. Schuerman Department of Biosciences 109 Tucker Hall University of Missouri at Columbia Columbia, MO 65211 office: schuermanp@missouri.edu home: schuerman@biosci.org "Who walks with truth, generates life." - Sumerian proverb --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Mar 16 16:47:13 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 023C717AB5; Thu, 16 Mar 2000 16:46:58 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 8F65B17A93; Thu, 16 Mar 2000 16:46:55 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: tenhaken@rhrk.uni-kl.de (Raimund Tenhaken) Subject: Soybean Two-Hybrid library Date: 16 Mar 2000 12:13:13 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear all, we are looking for a Two-Hybrid cDNA-library from soybean with Gal4 AD. Does anybody have such a library and is willing to share it with us? We can offer a high-quality expression cDNA-library that contains long full-length cDNAs. Thanks for any help Raimund Tenhaken *************************** Raimund Tenhaken Universität Kaiserslautern FB Biologie, Geb. 22 D-67663 Kaiserslautern Tel: +49 631 205 3040 Fax: +49 631 205 2600 *************************** --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Mar 17 00:23:12 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 5825A17AAA; Fri, 17 Mar 2000 00:23:09 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 47A0617A5C; Fri, 17 Mar 2000 00:23:07 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: bfxu@hotmail.com ("Bingfang Xu") Subject: nuclear protein extract kit? Date: 16 Mar 2000 16:56:43 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <20000316165602.19477.qmail@hotmail.com> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hi! Guys Would you please do us a favor to tell ue which company produce the "plant nuclear protein extract kit"? How to order it? Or whatever suggestion about the protocol of it you prefer and how can get the information of this kind kit. We are looking forward to hear from you! Thaanks a lot! Nan & bingfang e-mail: nr4g@virginia.edu ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Mar 17 20:22:10 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 4858D17A90; Fri, 17 Mar 2000 20:22:07 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 17DCE17A5D; Fri, 17 Mar 2000 20:22:05 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: mlodhi@biocept-inc.com ("Muhammad A. Lodhi") Subject: Positions in Genomics Dept. Date: 17 Mar 2000 16:46:35 -0000 Organization: Biocept, Inc. Message-ID: <38D26055.7ED3C683@biocept-inc.com> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Two openings in Genomics Department

We have two new positions open in our Genomics Department starting from April, 2000.  First position is for a Protein Biochemist to work on, but not limited to, protein-protein interactions in a novel microarray system.  An ideal candidate has a Ph.D. in biochemistry, molecular biology or related fields and experienced in working with protein expression, purification and analysis.  The
second position is for a research associate with a BS or MS.  Candidate for this position should have experience using basic molecular biology in his/her projects and can work with minimum supervision.  Preference will be given to candidates familiar with computational biology techniques.

Biocept provides an exciting and motivating environment for research work and is located in Carlsbad, about 40 miles North of San Diego.  To be considered for either of these positions please forward your resume to the following;
 

Muhammad A. Lodhi, Ph.D.
Director, Genomics
Biocept, Inc.
2151 Las Palmas Drive, Suite C
Carlsbad, CA 92009
Fax: 760-804-6238
E-mail: mlodhi@biocept-inc.com From owner-arab-gen@hgmp.mrc.ac.uk Mon Mar 20 18:22:36 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 5BA0717ABF; Mon, 20 Mar 2000 18:22:31 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id CBE1717A61; Mon, 20 Mar 2000 18:22:27 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: yinli@iris.sipp.ac.cn (Li Ying) Subject: spm mutants Date: 20 Mar 2000 06:00:53 -0000 Organization: Shanghai Institute of Plant Physiology , Chinese Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hi, friends: I have two En/I insertion mutants. Can anybody tell me wether I could select the mutants progeny on the antibiotics plates? If in this case, which antibiotics should I use , Km or Hygromycin? How can I stablize the I element? Dong Zhi-cheng Shanghai Institute of Plant Physiology, Chinese Academy of Science 300 Fenglin Rd. Shanghai 200040 --- From owner-arab-gen@hgmp.mrc.ac.uk Mon Mar 20 18:22:54 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id D01B917AC0; Mon, 20 Mar 2000 18:22:50 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 53F2D17ABF; Mon, 20 Mar 2000 18:22:49 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: laurent.cournac@cea.fr ("Laurent Cournac") Subject: Arabidopsis halleri Date: 20 Mar 2000 11:32:29 -0000 Organization: (CEA) Commissariat ý l'Energie Atomique, France. Message-ID: <8b527r$nv1$1@ellebore.saclay.cea.fr> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear netters, do somebody know where I could find or purchase seddds of the wild plant Arabidopsis halleri. Many thanks, Laurent Cournac -- ************************************************************ Dr. Laurent Cournac Laboratoire d'Ecophysiologie de la PhotosynthËse DEVM Bat 161 CEA, Centre de Cadarache 13108 Saint Paul Lez Durance Cedex FRANCE TÈl : 33 (0) 4 42 25 43 66 Fax : 33 (0) 4 42 25 62 65 email : laurent.cournac@cea.fr ************************************************************ --- From owner-arab-gen@hgmp.mrc.ac.uk Tue Mar 21 00:02:15 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 3123817ACA; Tue, 21 Mar 2000 00:02:12 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id F3DFD17A56; Tue, 21 Mar 2000 00:02:10 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: ouellettr@EM.AGR.CA (Thérèse Ouellet) Subject: postdoc job in fungal genomics Date: 20 Mar 2000 20:30:01 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Molecular Genetics Eastern Cereal and Oilseed Research Centre (ECORC) Agriculture and AgriFood Canada Ottawa, Ontario K1A 0C6 Canada One postdoctoral position is available now in the area of molecular pathology, to study the interactions between Fusarium graminearum and its hosts, maize (Gibberella ear rot) and wheat (Fusarium head blight). The fungal genes necessary for the plant/pathogen interaction will be the main focus of this project. We are looking for candidates combining knowledge and experience in molecular techniques and in pathology/mycology. Applications should be submitted to Therese Ouellet or Linda Harris, ECORC, via mail, fax or email before May 15 2000. Enquiries: Therese Ouellet, ECORC, Phone (613) 759-1658 Fax (613) 759-1701 E-mail ouellettr@em.agr.ca Linda Harris, ECORC, phone (613) 759-1314 Fax (613)759-6566 E-mail harrislj@em.agr.ca --- From owner-arab-gen@hgmp.mrc.ac.uk Tue Mar 21 19:12:02 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 3258817B05; Tue, 21 Mar 2000 19:11:56 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id EE5D917AC2; Tue, 21 Mar 2000 19:11:54 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: tamara@waksman.Rutgers.EDU (Tamara Western) Subject: testing dormancy Date: 21 Mar 2000 18:50:08 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <38D7C451.7FA5D516@mbcl.rutgers.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hi all, I am interested in checking seed dormancy for some of my plants. I was hoping someone out there could tell me at what stage should I harvest seeds in order to do this test (ie while siliques still yellow? do I need to watch to get them the day the siliques turn brown ...? ). thanks, Tamara -- Dr. Tamara L. Western Waksman Institute Rutgers University 190 Frelinghuysen Rd. Piscataway NJ 08854 tamara@waksman.rutgers.edu ____________ I wish I were a jelly fish That cannot fall downstairs -- G. K. Chesterton --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Mar 23 16:33:44 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 0B42317A7C; Thu, 23 Mar 2000 16:33:40 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id E237617A69; Thu, 23 Mar 2000 16:33:38 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: hughes@zedat.fu-berlin.de (Jon Hughes) Subject: PhD, FU Berlin: Phytochrome Date: 22 Mar 2000 16:53:43 -0000 Organization: Freie Universitaet Berlin Message-ID: <38d8fa15.112059252@news> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk I currently have a number of positions open in my group (in particular for a PhD student) to work on the Cph1 phytochrome from the cyanobacterium Synechocystis (see Hughes et al. 1997, Nature, 386: 663; Hughes & Lamparter, 1999, Plant Physiol., 121, 1059-1068). We use molecular-genetic (expression cloning, in vitro mutagenesis, protein chromatography, crystallography) and other methods to study the structure and function of this photoreceptor. In the process, we collaborate with other groups in a newly-established special research program studying protein structure/function (SFB 498) as well as with numerous colleagues throughout the world. In my lab I stress the importance of a friendly atmosphere, not only excellent science. (See also www.userpage.fu-berlin.de/~hughes/) - PhD (Doktorandenstelle, BAT2a/2) - Master's and short-term projects (Diplomarbeiten; freie Mitarbeit) Please contact me as soon as possible PD Dr Jon Hughes, Lab 228 Plant Physiology Free University of Berlin K–nigin-Luise-Str. 12-16 14195 Berlin / Dahlem Germany. Tel.: +49/0 30 838 54308 Fax.: +49/0 30 838 54357 email: hughes@zedat.fu-berlin.de --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Mar 23 16:35:21 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id EBD5017A7F; Thu, 23 Mar 2000 16:35:17 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 7DC7D17A7C; Thu, 23 Mar 2000 16:35:16 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: pikaard@biology.wustl.edu (Craig Pikaard) Subject: Ribosome/nucleolar function conference Date: 22 Mar 2000 21:38:09 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear colleagues, I'd like to call to the attention of the Arabidopsis and plant biology research communities a conference on the topic of ribosome biogenesis and nucleolar function. The meeting will occur August 17-21 at Lake Tahoe, California. A website describing the conference can be found at the following URL: http://www.umbc.edu/biosci/Ribosomes/ Please visit the website for information on how to be added to the mailing list. Plant labs working on such things as cytogenetic analyses of rRNA genes and NORs, ribosomal RNA synthesis and processing, ribosomal protein synthesis and regulation (both nuclear and plastid-encoded), ribosome inactivating proteins, and other related topics are encouraged to attend. Most speakers will be chosen from submitted abstracts, and poster sessions will provide additional opportunities for sharing results. Session topics are expected to include: Expression and regulation of rRNA and ribosomal protein genes Maturation of rRNA - processing, methylation, pseudouridylation snoRNP structure, formation and function Structure and function of the nucleolus and its components Import/export/localization of ribosomes and component proteins/RNAs Assembly of the ribosome machine Genomics and evolution of ribosomes and ribosome biogenesis Ribosome and nucleolar relevance to development and disease The meeting will cover all experimental systems, including bacteria, Archaea, Eucarya, and organelles. Increased participation from the plant biology community is strongly encouraged! Craig S. Pikaard Associate Professor Biology Department, Washington University Campus Box 1137, One Brookings Drive St. Louis, MO 63130 Ph: 314-935-7569, FAX:314-935-4432 e-mail: pikaard@biology.wustl.edu www: http://biosgi.wustl.edu/faculty/pikaard.html --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Mar 23 16:35:55 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id DC39E17A94; Thu, 23 Mar 2000 16:35:47 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id BE10C17A7F; Thu, 23 Mar 2000 16:35:44 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: mjchen@iris.sipp.ac.cn ("mingjie chen") Subject: metaphor agarose Date: 23 Mar 2000 08:39:30 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <000701bf94a3$22f49e60$0c2e0b0b@xdxu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk

Hi,friends:
 
Could anyone know which company sell metaphor agarose gel for electrophesis?My E.mail is:mjchen@iris.sipp.ac.cn< /FONT>
 
Thanks lot.
 
Mingjie Chen
 From owner-arab-gen@hgmp.mrc.ac.uk Thu Mar 23 16:36:33 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id B5D0817AB4; Thu, 23 Mar 2000 16:36:27 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 85E3717A94; Thu, 23 Mar 2000 16:36:26 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: naasuki@hotmail.com ("Kiran") Subject: QUESTION: Brassinosteroids and flowering Date: 23 Mar 2000 14:33:43 -0000 Organization: unknown Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Does anybody know if there are any known effects of brassinosteroids on flowering time in Arabidopsis or other plant species? Can you suggest an article to use as a starting point? Matej Lexa m-lexa@sci.muni.cz --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Mar 24 18:04:55 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 62E0817ABB; Fri, 24 Mar 2000 18:04:52 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id B233F17A8D; Fri, 24 Mar 2000 18:04:50 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: bstankovic@facstaff.wisc.edu (Bratislav Stankovic) Subject: Postdoc position in Molecular Biology at UW- Madison Date: 24 Mar 2000 16:21:48 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <4.2.2.20000324102315.00a56b60@facstaff.wisc.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Postdoctoral (Research Associate) position in Plant Molecular Biology available at the University of Wisconsin - Madison The Wisconsin Center for Space Automation and Robotics (WCSAR), located at the College of Engineering, University of Wisconsin Madison, has a postdoctoral position for broadly trained plant molecular biologist to become part of a team effort focused on development of novel technologies for plant transformation aimed to increase the biosynthesis of secondary metabolites. WCSAR's commercially-oriented research is conducted terrestrially, on the Space Shuttle, and on the International Space Station. We are particularly interested in applicants with experience in plant transformation and plant cell and tissue culture. A proven record of productivity including first-authored publications in internationally recognized journals will be a plus. The successful candidate will interact with other scientists and engineers in a team-oriented, collaborative approach. The Wisconsin Center for Space Automation and Robotics offers a unique opportunity for interfacing biotechnology, bioengineering and microgravity research. Information on WCSAR is available at http://wcsar.engr.wisc.edu. This is a one-year appointment, renewable for a period of up to 3 years. Salary: minimum $30,000. The University of Wisconsin is an Equal Opportunity Employer. Madison, a vibrant and colorful city located on an isthmus and surrounded by 4 lakes, is frequently referred to as one of the best places for living, working, and playing in the U.S.A. Information on Madison is available at http://www.ci.madison.wi.us If interested in research that is out of this world, send applications including a current CV, brief statement of research experience and interest, and names and addresses of 3 references to: Bratislav Stankovic, University of Wisconsin, Wisconsin Center for Space Automation and Robotics (WCSAR), 2352 Engineering Hall, Madison, WI 53706. Tel. (608) 265-8247; Fax: (608) 262-9458; E-mail: bstankovic@facstaff.wisc.edu --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Mar 24 18:05:33 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 8ED4C17AE4; Fri, 24 Mar 2000 18:05:28 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id C2FD117ABB; Fri, 24 Mar 2000 18:05:25 +0000 (GMT) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: bstankovic@facstaff.wisc.edu (Bratislav Stankovic) Subject: Postdoc position in Analytical Phytochemistry at UW- Madison Date: 24 Mar 2000 16:21:52 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <4.2.2.20000324102240.00a5a690@facstaff.wisc.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Postdoctoral (Research Associate) position in Analytical Phytochemistry available at the University of Wisconsin - Madison The Wisconsin Center for Space Automation and Robotics (WCSAR), located at the College of Engineering, University of Wisconsin Madison, has a postdoctoral position for a broadl