From owner-arab-gen@hgmp.mrc.ac.uk Thu Jun 1 17:01:48 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 13EC017B7F; Thu, 1 Jun 2000 17:00:32 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 89A7517BA2; Thu, 1 Jun 2000 16:55:11 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: Fejes.nb.szbk@nucleus.szbk.u-szeged.hu (Fejes ErzsThetabet) Subject: Re: Arbidopsis Callus Date: 1 Jun 2000 06:37:27 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <20CC4551DAE@nucleus.szbk.u-szeged.hu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hi Mauro! This is how I did it: Prepare Callus Inducing Medium (CIM) in Petri dishes: MS salts 2% sucrose pH adjusted to 5.7-5.8 with KOH 0.2 mg/l kinetin 1 mg/l 2,4-D 0.2% Phytagel Prepare AM medium, also in Petri dishes: 0.5x MS salts 1% sucrose pH to 5.7-5.8 with KOH 0.2% Phytagel Germinate sterilized Arabidopsis seed on AM medium, in the light. After 10 days you should have seedlings with expanded cotyledons and the first pair of true leaves just starting to show. Excise leaf and/or hypocotyl pieces (you should do this under water in a Petri dish, otherwise the seedlings dry out before you are finished) and lay them on solidified AM medium. Place them in light and callus growth should start soon. If you need more details, please ask - I cannot know what your previous experience is with this type of work. Good luck: Zso Fejes Institute of Plant Biology Biological Research Center H-6701 Szeged, P.O.Box 521. Ph. 36-62-432080/287 P.S. Your e-mail address doesn't seem to accept messages sent using the reply function. --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Jun 1 19:51:08 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id A9B8A17B49; Thu, 1 Jun 2000 19:51:04 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 891EB17B4B; Thu, 1 Jun 2000 19:51:03 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis Subject: Arabidopsis seeds Date: Thu, 01 Jun 2000 16:00:26 +0200 Organization: NASK - http://www.nask.pl Message-ID: <39366C79.EF05B974@alpha.sggw.waw.pl> From: nobody@hgmp.mrc.ac.uk (Nobody) Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk What is the higest temperature, in which Arabidopsis seeds can survive? From owner-arab-gen@hgmp.mrc.ac.uk Thu Jun 1 19:51:45 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 3298317B49; Thu, 1 Jun 2000 19:51:41 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id C99DC17B4C; Thu, 1 Jun 2000 19:51:39 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: chenauxp@biology.queensu.ca (Peter Chenaux) Subject: Arabadopsis library Date: 1 Jun 2000 16:22:50 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <3.0.5.32.20000601105549.007c6870@biology.queensu.ca> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hello, I was wondering if anyone knew of a yeast two-hybrid library (or a library that can be turned into a 2-hybrid) that contained root tissue as well as above ground tissue. I'm using the LexA system, and would prefer a library in a compatible vector, but I am willing to construct new bait vectors if a compatible library isn't found. Any help would be greatly appreciated as library sceening is completely new to me. Thanks in advance, Peter Chenaux Peter R Chenaux, Rm.3515 DEPARTMENT OF BIOLOGY QUEEN'S UNIVERSITY KINGSTON, ONTARIO K7L 3N6 CANADA chenauxp@biology.queensu.ca Tele:(613)533-6000 ext. 77611 Fax:613-533-6617 --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Jun 1 19:52:20 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 0870D17B54; Thu, 1 Jun 2000 19:52:15 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 6FFAC17B4F; Thu, 1 Jun 2000 19:52:13 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: memlab@uchile.cl ("Laboratorio de Membranas") Subject: Tubulin and actin purification Date: 1 Jun 2000 19:41:02 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <000d01bfcc09$858c4e40$283a5392@Helmholtz.ciencias.uchile.cl> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk
I am a PHD. student from Chile and I am just starting to work in Arabidopsis
I would like to ask for protocols about Tubulin and Actin purification from Arabidopsis or any other plant
If any one can help me I will be very gratefull,
Thanks
Alejandra Bustos
 From owner-arab-gen@hgmp.mrc.ac.uk Thu Jun 1 21:28:23 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 21CE317B41; Thu, 1 Jun 2000 21:28:19 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 3A50017B41; Thu, 1 Jun 2000 21:28:16 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: Testr@pz.uni-hohenheim.de (Testr Uni) Subject: heterosis studies in arabidopsis Date: 1 Jun 2000 20:19:57 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <3936B75A.E8B0DF44@pz.uni-hohenheim.de> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hello everyone, I am a graduate student at the University of Hohenheim,Germany and will be working with arabidopsis for my thesis project. I would like to hav more information on "Heterosis studies in Arabidopsis" . I would appreciate any help anyone can give me, references or anything. Thank you in advance ! Anil Kumar , Busimi anilkb99@yahoo.com --- From owner-arab-gen@hgmp.mrc.ac.uk Sat Jun 3 22:18:10 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 4E04E17B26; Sat, 3 Jun 2000 22:18:07 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id B9A7917B2E; Sat, 3 Jun 2000 22:18:05 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: curator@arabidopsis.org Subject: New/Updated Arabidopsis DNA 03-Jun-2000 Date: 3 Jun 2000 21:51:20 +0100 Organization: The Arabidopsis Information Resource Message-ID: <200006032051.OAA24095@cleveland.ncgr.org> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Arabidopsis researchers, The following information is the number of Arabidopsis entries in GenBank that are new or were updated in the last week. These sequences have been updated in TAIR and are available for searching using TAIR BLAST and FASTA at: http://arabidopsis.org/blast/ http://arabidopsis.org/cgi-bin/fasta/TAIRfasta.pl New sequences : 37 Updated sequences : 0 Updated annotation : 54 ====================== New DNA Sequences ======================= AF135189 AF135189 2271bp mRNA PLN 27-APR-2000 Arabidopsis thaliana zinc dependent protease (VAR2) mRNA, complete cds; nuclear gene for chloroplast product. VAR2; zinc dependent protease. AC069159 AC069159 112446bp DNA HTG 31-MAY-2000 Arabidopsis thaliana chromosome I clone IGF-F14G9, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC069160 AC069160 100954bp DNA HTG 31-MAY-2000 Arabidopsis thaliana chromosome I clone TAMU-T15K4, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC069299 AC069299 101488bp DNA HTG 25-MAY-2000 Arabidopsis thaliana chromosome I clone TAMU-T1E4, *** SEQUENCING IN PROGRESS ***, 12 unordered pieces. HTG; HTGS_PHASE1. AF186466 AF186466 7317bp DNA PLN 25-MAY-2000 Arabidopsis thaliana non-phototropic hypocotyl 4 (NPH4) gene, complete cds. non-phototropic hypocotyl 4; NPH4. ATH242671 AJ242671 1628bp RNA PLN 27-MAY-2000 Arabidopsis thaliana mRNA for putative serine threonine kinase (aak1 gene) AAK1 protein; aak1 gene; serine threonine kinase; putative serine threonine kinase; aak1. AC069325 AC069325 85237bp DNA HTG 27-MAY-2000 Arabidopsis thaliana chromosome 5 clone T10F18, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces. HTG; HTGS_PHASE1. AC069327 AC069327 94287bp DNA HTG 27-MAY-2000 Arabidopsis thaliana chromosome 5 clone T26G08, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces. HTG; HTGS_PHASE1. AC069329 AC069329 165942bp DNA HTG 27-MAY-2000 Arabidopsis thaliana chromosome 5 clone T3P01, *** SEQUENCING IN PROGRESS ***, 27 unordered pieces. HTG; HTGS_PHASE1. AB035288 AB035288 2577bp mRNA PLN 30-MAY-2000 Arabidopsis thaliana fkfbp mRNA for fructose-6-phosphate, 2-kinase/fructose-2,6-bisphosphatase, complete cds. Fructose-6-phosphate,2-kinase/Fructose-2,6-bisphosphatase; fructose-6-phosphate,2-kinase/fructose-2, 6-bisphosphatase; fkfbp. AF262733 AF262733 972bp mRNA PLN 30-MAY-2000 Arabidopsis thaliana putative transcription factor MYB108 (MYB108) mRNA, complete cds. putative transcription factor MYB108; MYB108. AF262734 AF262734 1200bp mRNA PLN 30-MAY-2000 Arabidopsis thaliana putative transcription factor MYB109 (MYB109) mRNA, complete cds. putative transcription factor MYB109; MYB109. AF263518 AF263518 10989bp DNA PLN 31-MAY-2000 Arabidopsis thaliana protein synthesis initiation factor 4G (EIF4G) gene, complete cds. protein synthesis initiation factor 4G; EIF4G. AF263834 AF263834 452bp mRNA PLN 31-MAY-2000 Arabidopsis thaliana protein synthesis initiation factor 4G (EIF4G) mRNA, partial cds. protein synthesis initiation factor 4G; EIF4G. AF148541 AF148541 2581bp mRNA PLN 01-JUN-2000 Arabidopsis thaliana cyclic nucleotide-binding transporter 1 (CNBT1) mRNA, complete cds. CNBT1; cyclic nucleotide-binding transporter 1. AF148542 AF148542 9070bp DNA PLN 01-JUN-2000 Arabidopsis thaliana cyclic nucleotide-binding transporter 2 (CNBT2) and cyclic nucleotide-binding transporter 1 (CNBT1) genes, complete cds. cyclic nucleotide-binding transporter 2; CNBT2; cyclic nucleotide-binding transporter 1; CNBT1. AF148852 AF148852 1775bp mRNA PLN 01-JUN-2000 Arabidopsis thaliana 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) mRNA, complete cds. DXR; 1-deoxy-D-xylulose 5-phosphate reductoisomerase. AF153443 AF153443 1793bp mRNA PLN 01-JUN-2000 Arabidopsis thaliana phytochrome A signal transduction 1 protein (PAT1) mRNA, complete cds. PAT1; phytochrome A signal transduction 1 protein. AF155171 AF155171 6570bp DNA PLN 01-JUN-2000 Arabidopsis thaliana flavonoid 3'-hydroxylase (TT7) gene, complete cds. flavonoid 3'-hydroxylase; TT7. AF213627 AF213627 6554bp mRNA PLN 01-JUN-2000 Arabidopsis thaliana MOM mRNA, complete cds. MOM. AF213628 AF213628 10329bp DNA PLN 01-JUN-2000 Arabidopsis thaliana MOM gene, complete cds. MOM. IG007P01 AF272706 110714bp DNA PLN 01-JUN-2000 Arabidopsis thaliana BAC IG007P01. ATH271731 AJ271731 1332bp RNA PLN 01-JUN-2000 Arabidopsis thaliana mRNA for branched-chain amino acid transaminase (bcat2 gene) bcat2 gene; branched-chain amino acid transaminase; bcat2. ATH271732 AJ271732 1286bp RNA PLN 01-JUN-2000 Arabidopsis thaliana mRNA for branched-chain amino acid transaminase (bcat4 gene) bcat4 gene; branched-chain amino acid transaminase; bcat4. ATH276123 AJ276123 1368bp RNA PLN 01-JUN-2000 Arabidopsis thaliana mRNA for branched-chain amino acid transaminase (bcat1 gene) bcat1 gene; branched-chain amino acid transaminase; bcat1. ATH276124 AJ276124 1539bp RNA PLN 01-JUN-2000 Arabidopsis thaliana mRNA for branched-chain amino acid transaminase 3 (bcat3 gene) bcat3 gene; branched-chain amino acid transaminase 3; bcat3. AF207994 AF207994 2304bp mRNA PLN 02-JUN-2000 Arabidopsis thaliana acyl-CoA oxidase ACX3 (ACX3) mRNA, complete cds. ACX3; acyl-CoA oxidase ACX3. AF223949 AF223949 2165bp mRNA PLN 02-JUN-2000 Arabidopsis thaliana amidase mRNA, complete cds. amidase. AF229850 AF229850 2972bp DNA PLN 02-JUN-2000 Arabidopsis thaliana ferritin (Fer1) gene, complete cds. Fer1; ferritin. AF236376 AF236376 542bp mRNA PLN 02-JUN-2000 Arabidopsis thaliana IPS1 mRNA, complete sequence. IPS1. AF239718 AF239718 4013bp DNA PLN 02-JUN-2000 Arabidopsis thaliana SGS2 gene, complete cds. SGS2. AF239719 AF239719 2254bp DNA PLN 02-JUN-2000 Arabidopsis thaliana SGS3 gene, complete cds. SGS3. AC069470 AC069470 291898bp DNA HTG 01-JUN-2000 Arabidopsis thaliana chromosome I clone IGF-F13E17, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces. HTG; HTGS_PHASE1. AC069471 AC069471 127042bp DNA HTG 01-JUN-2000 Arabidopsis thaliana chromosome I clone IGF-F13K9, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC069472 AC069472 52211bp DNA HTG 01-JUN-2000 Arabidopsis thaliana chromosome III clone BAC pool-F28J15, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC069473 AC069473 85822bp DNA HTG 01-JUN-2000 Arabidopsis thaliana chromosome III clone BAC pool-T21B14, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC069474 AC069474 106390bp DNA HTG 01-JUN-2000 Arabidopsis thaliana chromosome III clone BAC pool-T2E22, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. =========== Updated Sequence Features/Annotations ============= AC000348 AC000348 111566bp DNA PLN 20-MAY-2000 Genomic sequence for Arabidopsis thaliana BAC T7N9 from chromosome I, complete sequence. HTG. D85029 D85029 1245bp ss-mRNA PLN 31-MAY-2000 Arabidopsis thaliana mRNA for geranylgeranyl pyrophosphate synthase, partial cds. geranylgeranyl pyrophosphate synthase. AC002328 AC002328 109171bp DNA PLN 13-MAY-2000 Genomic sequence for Arabidopsis thaliana BAC F20N2 from chromosome I, complete sequence. HTG. AB005231 AB005231 79537bp DNA PLN 27-MAY-2000 Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone:MBB18. HTG. AB000835 AB000835 3250bp DNA PLN 31-MAY-2000 Arabidopsis thaliana gene for geranyl geranyl pyrophosphate synthase, complete cds. geranyl geranyl pyrophosphate synthase; GGPS6. AF091843 AF091843 1485bp mRNA PLN 25-MAY-2000 Arabidopsis thaliana aminoalcoholphosphotransferase (AAPT1) mRNA, complete cds. AAPT1; aminoalcoholphosphotransferase. AF091844 AF091844 1314bp mRNA PLN 25-MAY-2000 Arabidopsis thaliana aminoalcoholphosphotransferase (AAPT2) mRNA, complete cds. AAPT2; aminoalcoholphosphotransferase. AC006193 AC006193 118335bp DNA PLN 28-MAY-2000 Arabidopsis thaliana chromosome I BAC F13O11 genomic sequence, complete sequence. HTG; F13O11.1; F13O11.2; F13O11.3; Putative membrane related protein; F13O11.4; alpha1 tubulin; F13O11.5; F13O11.6; Similar to glucan endo-1,3-beta-glucosidase precursor; F13O11.7; F13O11.8; Ammonium transporter ATM1;2; F13O11.9; similar to translational activator; F13O11.10; F13O11.11; F13O11.12; F13O11.13; F13O11.14; F13O11.15; RNA polymerase sigma subunit 1; F13O11.16; F13O11.17; F13O11.18; F13O11.19; Putative Cytochrome P450; F13O11.20; Similar to Flavonol 3-O-Glucosyltransferase; F13O11.21; F13O11.22; F13O11.23; Putative cytochrome P450; F13O11.24; F13O11.25; F13O11.26; gamma-tocopherol methyltransferase; F13O11.27; F13O11.28; similar to CGI-13 protein; F13O11.29; F13O11.30. AC007152 AC007152 90388bp DNA HTG 24-MAY-2000 Arabidopsis thaliana chromosome I clone F1O19, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC007945 AC007945 80472bp DNA PLN 26-MAY-2000 Genomic sequence for Arabidopsis thaliana BAC F28C11 from chromosome I, complete sequence. HTG. AC008007 AC008007 130235bp DNA PLN 25-MAY-2000 Genomic sequence for Arabidopsis thaliana BAC F12M16 from chromosome I, complete sequence. HTG; F12M16.1; F12M16.2; F12M16.3; F12M16.4; F12M16.5; F12M16.6; F12M16.7; F12M16.8; F12M16.9; F12M16.10; F12M16.11; F12M16.12; tRNA-Ile; F12M16.13; F12M16.14; F12M16.15; F12M16.16; F12M16.17; F12M16.18; F12M16.19; F12M16.20; F12M16.21; F12M16.22; F12M16.23; F12M16.24; F12M16.25; F12M16.26; F12M16.27; F12M16.28; F12M16.29; tRNA-Cys; F12M16.30. AB015859 AB015859 588bp mRNA PLN 25-MAY-2000 Arabidopsis thaliana psbS mRNA for photosystem II 22 kDa protein, partial cds. photosystem II 22 kDa protein; psbS. AB015860 AB015860 295bp mRNA PLN 25-MAY-2000 Arabidopsis thaliana psaE mRNA for photosystem I subunit PSI-E, partial cds. photosystem I subunit PSI-E; psaE. AB015861 AB015861 512bp mRNA PLN 25-MAY-2000 Arabidopsis thaliana psaL mRNA for photosystem I subunit PSI-L, partial cds. photosystem I subunit PSI-L; psaL. AB015862 AB015862 240bp mRNA PLN 25-MAY-2000 Arabidopsis thaliana mRNA for phytochrome-regulated gene product, partial sequence. AB030730 AB030730 1701bp DNA PLN 27-MAY-2000 Arabidopsis thaliana AZF2 gene for Cys2/His2-type zinc finger protein 2, complete cds. Cys2/His2-type zinc finger protein 2; AZF2. AB030731 AB030731 735bp DNA PLN 27-MAY-2000 Arabidopsis thaliana AZF1 gene for Cys2/His2-type zinc finger protein 1, partial cds. Cys2/His2-type zinc finger protein 1; AZF1. AB030732 AB030732 579bp DNA PLN 27-MAY-2000 Arabidopsis thaliana AZF3 gene for Cys2/His2-type zinc finger protein 3, partial cds. Cys2/His2-type zinc finger protein 3; AZF3. AC011810 AC011810 103157bp DNA HTG 01-JUN-2000 Arabidopsis thaliana chromosome I BAC T6J4 genomic sequence, complete sequence. HTG. AC013430 AC013430 95769bp DNA PLN 26-MAY-2000 Genomic sequence for Arabidopsis thaliana BAC F3F9 from chromosome I, complete sequence. HTG; F3F9.1; F3F9.2; F3F9.3; F3F9.4; F3F9.5; F3F9.6; F3F9.7; F3F9.8; F3F9.9; F3F9.10; F3F9.11; F3F9.12; F3F9.13; F3F9.14; F3F9.15; F3F9.16; F3F9.17; F3F9.18; F3F9.19; F3F9.20; tRNA-Met; F3F9.21; F3F9.22. AC013453 AC013453 91001bp DNA PLN 25-MAY-2000 Arabidopsis thaliana chromosome I BAC T16N11 genomic sequence, complete sequence. HTG; Putative adenine nucleotide translocase; T16N11.1; T16N11.2; Putative ABC transporter; T16N11.3; T16N11.4; T16N11.5; GA4 protein; T16N11.6; T16N11.7; Putative cyclin; T16N11.8; auxin-induced protein AUX2-27; T16N11.9; T16N11.10; T16N11.11; T16N11.12; T16N11.13; T16N11.14; T16N11.15; T16N11.16. AF210731 AF210731 2130bp mRNA PLN 30-MAY-2000 Arabidopsis thaliana scarecrow-like 1 (SCL1) mRNA, complete cds. SCL1; scarecrow-like 1. AF210732 AF210732 1634bp mRNA PLN 30-MAY-2000 Arabidopsis thaliana scarecrow-like 21 (SCL21) mRNA, complete cds. SCL21; scarecrow-like 21. AF219222 AF219222 1342bp DNA PLN 22-MAY-2000 Arabidopsis thaliana homocysteine S-methyltransferase AtHMT-1 (AtHMT-1) gene, complete cds. homocysteine S-methyltransferase AtHMT-1; AtHMT-1. AF219223 AF219223 1391bp DNA PLN 22-MAY-2000 Arabidopsis thaliana homocysteine S-methyltransferase AtHMT-2 (AtHMT-2) gene, complete cds. homocysteine S-methyltransferase AtHMT-2; AtHMT-2. AF206324 AF206324 2431bp mRNA PLN 30-MAY-2000 Arabidopsis thaliana putative DNA binding protein (tso1) mRNA, tso1-3 allele, complete cds. tso1; putative DNA binding protein. AF151217 AF151217 1608bp mRNA PLN 30-MAY-2000 Arabidopsis thaliana proline-rich protein (PRP4) mRNA, complete cds. PRP4; proline-rich protein. AC022521 AC022521 121668bp DNA PLN 20-MAY-2000 Arabidopsis thaliana chromosome I BAC T14P4 genomic sequence, complete sequence. HTG. AC024226 AC024226 122905bp DNA HTG 20-MAY-2000 Arabidopsis thaliana chromosome 5 clone T32B03, *** SEQUENCING IN PROGRESS ***, 22 unordered pieces. HTG; HTGS_PHASE1. AC025813 AC025813 89390bp DNA HTG 31-MAY-2000 Arabidopsis thaliana chromosome I clone F15H4, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces. HTG; HTGS_PHASE1. AC027134 AC027134 102184bp DNA HTG 01-JUN-2000 Arabidopsis thaliana chromosome I clone F13B4, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC027656 AC027656 99328bp DNA HTG 20-MAY-2000 Arabidopsis thaliana chromosome 1 clone F21F23, *** SEQUENCING IN PROGRESS ***, 2 ordered pieces. HTG; HTGS_PHASE2. AC027665 AC027665 99231bp DNA PLN 26-MAY-2000 Genomic sequence for Arabidopsis thaliana BAC F5M15 from chromosome I, complete sequence. HTG. AC034256 AC034256 77521bp DNA PLN 27-MAY-2000 Arabidopsis thaliana chromosome 1 BAC F7H2 sequence, complete sequence. HTG. AC034257 AC034257 98950bp DNA PLN 01-JUN-2000 Arabidopsis thaliana chromosome I BAC F11A6 genomic sequence, complete sequence. HTG. AC036106 AC036106 51811bp DNA HTG 25-MAY-2000 Arabidopsis thaliana chromosome III clone TAMU-T8E24, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC037424 AC037424 76619bp DNA HTG 20-MAY-2000 Arabidopsis thaliana chromosome I clone IGF-F19K6, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC025416 AC025416 118374bp DNA PLN 26-MAY-2000 Genomic sequence for Arabidopsis thaliana BAC F5O11 from chromosome I, complete sequence. HTG. AC051625 AC051625 119039bp DNA HTG 20-MAY-2000 Arabidopsis thaliana chromosome 5 clone F19N02, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces. HTG; HTGS_PHASE1. AC051626 AC051626 96050bp DNA PLN 27-MAY-2000 Genomic Sequence For Arabidopsis thaliana Clone F20L16 From Chromosome V, complete sequence. HTG. AC051627 AC051627 85746bp DNA PLN 22-MAY-2000 Genomic Sequence For Arabidopsis thaliana Clone T1A4 From Chromosome V, complete sequence. HTG. AC051631 AC051631 97366bp DNA HTG 20-MAY-2000 Arabidopsis thaliana chromosome I clone TAMU-T2J15, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC061957 AC061957 66131bp DNA PLN 20-MAY-2000 Arabidopsis thaliana chromosome 1 BAC F22L4 sequence, complete sequence. HTG. AC064879 AC064879 109180bp DNA PLN 20-MAY-2000 Arabidopsis thaliana chromosome I BAC T6A9 genomic sequence, complete sequence. HTG. AC067971 AC067971 173520bp DNA HTG 27-MAY-2000 Arabidopsis thaliana chromosome 1 clone F10K1, *** SEQUENCING IN PROGRESS ***, 2 ordered pieces. HTG; HTGS_PHASE2. AC068143 AC068143 36844bp DNA PLN 01-JUN-2000 Arabidopsis thaliana chromosome 1 BAC T2D23 sequence, complete sequence. HTG. AC068562 AC068562 87048bp DNA HTG 26-MAY-2000 Arabidopsis thaliana chromosome 1 clone T16E15, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces. HTG; HTGS_PHASE1. AC068655 AC068655 96926bp DNA HTG 20-MAY-2000 Arabidopsis thaliana chromosome 5 clone F17K4, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces. HTG; HTGS_PHASE1. AC063973 AC063973 155386bp DNA HTG 27-MAY-2000 Arabidopsis thaliana chromosome 5 clone T6F08, *** SEQUENCING IN PROGRESS ***, 19 unordered pieces. HTG; HTGS_PHASE1. AC068901 AC068901 102744bp DNA HTG 27-MAY-2000 Arabidopsis thaliana chromosome I clone IGF-F1O3, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces. HTG; HTGS_PHASE1. AC068324 AC068324 96219bp DNA HTG 25-MAY-2000 Arabidopsis thaliana chromosome I clone IGF-F13A11, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC069273 AC069273 53025bp DNA HTG 24-MAY-2000 Arabidopsis thaliana chromosome I clone IGF-F28P5, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC069251 AC069251 139913bp DNA HTG 31-MAY-2000 Arabidopsis thaliana chromosome 1 clone F2D10, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces. HTG; HTGS_PHASE1. AC069252 AC069252 82717bp DNA HTG 25-MAY-2000 Arabidopsis thaliana chromosome 1 clone F2E2, *** SEQUENCING IN PROGRESS ***, 9 unordered pieces. HTG; HTGS_PHASE1. --- From owner-arab-gen@hgmp.mrc.ac.uk Tue Jun 6 15:51:55 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 1718C17AD4; Tue, 6 Jun 2000 15:51:48 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id C7D4317B20; Tue, 6 Jun 2000 15:51:46 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: ykamiya@postman.riken.go.jp (Yuji Kamiya) Subject: Postdoctoral position in Japan Date: 6 Jun 2000 07:34:20 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Arabidopsis scientist A postdoctoral position is available at RIKEN Plant Science Center, Japan from August 2000. RIKEN is located near Tokyo and about 20 min by train from downtown Tokyo. More than 2000 researchers are working at RIKEN institute and we have over 200 non-Japanese researchers. Please see our homepage. http://www.riken.go.jp/eng/index.html. The project is related to (1) regulation of GA and ABA biosynthesis and signal transduction in seeds (Arabidopsis and tomato) and (2) production of useful secondary metabolites in seeds. The project will prolong for 5 years. The positions are available for minimum 2 years and extension is possible till the end of March 2005. Those who are interested in this position, please contact Dr. Yuji Kamiya by e-mail Related papers to this project. *Hedden, P. and Kamiya, Y. (1997) Gibberellin biosynthesis: enzymes, genes and their regulation. Ann. Rev. Plant Physiol. Plant Mol. Biol. 48, 431-460 *Yamaguchi, S. et al. (1998) Phytochrome regulation and differential expression of gibberellin 3b-hydroxylase genes in germinating Arabidopsis seeds. Plant Cell 10, 2115-2126 *Rebers, M. et. al. (1999) Regulation of gibberellin biosynthesis genes during flower and early fruit development of tomato. Plant J. 17, 241-250 *Bishop, G. et al. (1999) The tomato DWARF enzyme is a bifunctional C-6 oxidase in brassinosteroid biosynthesis. Proc. Natl. Acad. Sci. USA 96, 1761-1766 *Ait-Ali, T. et al (1999) Regulation of GA 20-oxidase and GA3b-hydroxylase transcript accumulation during phytochrome-mediated de-etiolation of pea seedlings. Plant Physiol. 121, 783-791 *Kamiya, Y., Garcia-Martinez, J.L. (1999) Regulation of gibberellin biosynthesis by light. Current Opinion in Plant Biology 2, 398-403 *Yamaguchi, S., Kamiya, Y. (2000) Gibberellin Biosynthesis: Its Regulation by Endogenous and Environmental Signals. Plant Cell Physiol. 41, 251-257 ------------------------------------------------- Dr. Yuji Kamiya Plant Functions Lab. RIKEN (The Institute of Physical and Chemical Research) Hirosawa 2-1, Wako-shi, Saitama 351-0198 Japan Tel +81-48-462-9375 Fax +81-48-462-4691 E-mail: ykamiya@postman.riken.go.jp　 home page, http://frpphf.riken.go.jp/ --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Jun 7 16:06:31 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 04F9A17ABC; Wed, 7 Jun 2000 16:06:27 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id A807E17B23; Wed, 7 Jun 2000 16:06:26 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: cheongjj@snu.ac.kr ("Jong-Joo Cheong") Subject: DNA chip Date: 7 Jun 2000 08:18:57 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <007601bfd43e$acc02e40$485f2e93@snu.ac.kr> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk
Dear Arabinetters:
 
I have a question.
DNA chips with plant genes are available now, commercially or even privately ?
Or, can we expect when they are available ?
I am interested especially in the genes involved in plant defense responses.
It would be greatly appreciated if you would let me get such information.
Thank you in advance. 
 
---------------------------------
Jong-Joo Cheong, Ph.D.
School of Agricultural Biotechnology
Seoul National University
Suwon 441-744, KOREA
 
TEL : 011-759-9166
FAX : 0331-291-7011
E-mail : cheongjj@snu.ac.kr
----------- ----------------------
 From owner-arab-gen@hgmp.mrc.ac.uk Wed Jun 7 16:06:54 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 3F74317ABC; Wed, 7 Jun 2000 16:06:51 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 4271017B29; Wed, 7 Jun 2000 16:06:50 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: jagreet@ccmb.ap.nic.in (Jagreet Kaur) Subject: Antibodies against peptides?? Date: 7 Jun 2000 09:02:02 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hi I am working with a gene which is a member of a multigene family (4 Putative homologues acc to the database search) I want to design a specific antibody against my protein.I was told that it is possible to raise specific antibodies against small peptides too: But I donot know how to select for this peptide from my protein sequence. - Can I use hydrophilicity /hydrophobicity values derived using Dolittle's method to decide upon which peptide sequence would be good to use -How long a peptide is generally used for such purposes ? _Are there any programs which can predict the antigenicity of a given peptide/protein Any kind of help and suggestions regarding this problem would be greatly appreciated Thanks . Jagreet Kaur --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Jun 7 16:07:31 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id F346517B35; Wed, 7 Jun 2000 16:07:26 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id C011817B37; Wed, 7 Jun 2000 16:07:25 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: pscfr2@hermes.cam.ac.uk ("P. Rocha") Subject: Info on Binary Vectors Date: 7 Jun 2000 09:08:53 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hello, The integration patterns of T-DNAs (namely getting "single copy" versus "repeats" at a locus) depend on a variety of factors. Included are the VECTOR used, Agrobacterium strain used and the transformation method. I am seeking binary vectors (and strains of bacteria) that when used for transformation via infiltration will tend to yield transformants carrying a single T-DNA copy at the integration site (aren't we all?). If you have any tips on this, please share them. I would also be very happy to hear about vectors/strain combinations that upon transformation by this method would give the"undesirable" T-DNA repeats at the integration sites. I will collate and post back any replies as this information might be of general utility. Thanks in advance for your help. Pedro Rocha ----------------------------- E-mail: pscfr2@cam.ac.uk Fax:+44-1223-333992 --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Jun 9 15:17:15 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 2307A17A91; Fri, 9 Jun 2000 15:17:12 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 184D817A99; Fri, 9 Jun 2000 15:17:10 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: mark.thomas@pi.csiro.au (Mark Thomas) Subject: Postdoctoral positions at CSIRO, Australia Date: 9 Jun 2000 01:15:27 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Times_New_RomanPosition PG:99127 (PG:00127) 3 year term We are seeking a Post-doctoral fellow with experience in plant genetics and molecular biology to join a research group undertaking the genetic and molecular characterisation of a powdery mildew resistance gene in grapevine. The research will involve the identification and genetic mapping of molecular markers linked to the resistance gene and the localisation of these markers on high molecular weight DNA libraries for map-based cloning of the resistance gene. The successful candidate must have a strong background in genetic and/or physical mapping technologies and a PhD in plant genetics or plant molecular biology. Position PG:99128 (PG:00128) 3 year term. We are seeking a Post-doctoral fellow with experience in developmental plant genetics and molecular biology to join a project aimed at isolating and characterising genes involved in inflorescence development, with the strategic goal of manipulating bunch architecture in grapevines. The research will utilise model systems such as Arabidopsis to identify important genes involved in inflorescence development. The person will also investigate grapevine mutants with altered bunch architecture and interact with a project characterising grapevine floral genes and a project studying the inheritance of traits affecting grape bunch architecture. The successful candidate must have a PhD in plant genetics or plant molecular biology and a strong background in developmental biology and genetic mapping technologies. For further information and selection documentation please email kerry.summerfield@pi.csiro.au or visit our web site http://www.pi.csiro.au Applications close 16 June 2000. --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Jun 9 15:17:40 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 3D1F317A91; Fri, 9 Jun 2000 15:17:35 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id D2E5617AEC; Fri, 9 Jun 2000 15:17:33 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis Subject: PLANT-INSECT INTERACTIONS Date: 9 Jun 2000 01:41:24 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <4.2.0.58.20000608173721.03b04a40@ag.arizona.edu> From: nobody@hgmp.mrc.ac.uk (Nobody) Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk POSTDOCTORAL TRAINING IN THE FUNCTIONAL GENOMICS OF PLANT-INSECT INTERACTIONS. The NSF-supported Research Training Program in Plant-Insect Interactions at the University of Arizona invites applications from prospective postdoctoral fellows. We are particularly interested in identifying candidates to employ functional genomics to analyze the interactions of lepidopteran and homopteran herbivores with host plants. Individual projects include, but are not limited to, studies of gene expression (a) within generalist and specialist insect pests, (b) associated with insect digestive and olfactory processes, and (c) involved with the transmission of phytopathogenic agents. Salary range $25,500-$36,000 based on years of postdoctoral experience. Further information can be obtained from our website at http://ag.arizona.edu/pii-rtg or from Human Resources at http://www.hr.arizona.edu. To apply, send a statement of research accomplishments and aspirations, a r=E9sum=E9, and names of three references to: Plant-Insect Interactions Interdisciplinary Program, Attention: Dr. David Galbraith, 303 Forbes Building, University of Arizona, Tucson, AZ 85721. Please reference job number 17803. Review of applications is ongoing and will continue through December 31, 2000. The University of Arizona is an EEO/AA Employer-M/W/D/V. Please note: Applicants must be citizens or permanent residents of the United States. --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Jun 9 15:17:54 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id AF00F17A91; Fri, 9 Jun 2000 15:17:48 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 0398517AEC; Fri, 9 Jun 2000 15:17:46 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: hongyong@gate.sinica.edu.tw Subject: good antibody sources for GAL4 BD and AD domain Date: 9 Jun 2000 04:16:09 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <2.2.32.20000609031842.0069c1d4@gate.sinica.edu.tw> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Arabidopsis researchers: wondering who may know a good sources for GAL4 BD and AD domain antibodies that can be used to examine expression of AD BD fusions in yeast cells? we tried monoclone antibodies from clontech, and found out the detection sensitivity is quite low. Thanks! Hongyong /////// Hongyong Fu Assistant Research Fellow Room 422, Institute of Botany Academia Sinica Taipei Taiwan tel: 011-886-22782-3050 ext 422 fax:011-886-22782-7954 --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Jun 9 18:04:27 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 32D5617A8B; Fri, 9 Jun 2000 18:04:22 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id E3CB117A8D; Fri, 9 Jun 2000 18:04:19 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: campanellj@mail.montclair.edu ("James Campanell") Subject: Arabidopsis callus cultures Date: 9 Jun 2000 16:39:36 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <3.0.6.32.20000609113859.007c9560@mail.montclair.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Arabinauts: I'm looking for someone who is growing callus cultures of Arabidopsis. We are doing some histochemistry work with another tissue culture and we need an Arabidopsis wild-type culture for comparative purposes. We could easily induce such a culture ourselves, but we frankly need to start our work this summer and we just don't have the time to make this a DIY. Does anyone have the cultures available? I'd be grateful for any help. Thanks. Jim Campanella ------------------ Dr. Jim Campanella, Asst. professor Montclair State University Biology Dept. Montclair, New Jersey 07043 USA 973-655-4097 --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Jun 9 18:04:43 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 316D617A8B; Fri, 9 Jun 2000 18:04:37 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id D4C0F17B17; Fri, 9 Jun 2000 18:04:35 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: abbrands@artsci.wustl.edu (Alex Brands) Subject: Re: good antibody sources for GAL4 BD and AD domain Date: 9 Jun 2000 17:02:43 +0100 Organization: Washington University in St. Louis Message-ID: References: <2.2.32.20000609031842.0069c1d4@gate.sinica.edu.tw> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk On 9 Jun 2000 hongyong@gate.sinica.edu.tw wrote: > Dear Arabidopsis researchers: > > wondering who may know a good sources for GAL4 BD and AD domain antibodies > that can be used to examine expression of AD BD fusions in yeast cells? we > tried monoclone antibodies from clontech, and found out the detection > sensitivity is quite low. Thanks! Not only that, but their price is outrageous. I was searching for the same thing a few weeks ago, and found: http://www.santacruzbiotechnology.com/ Enter GAL4 in "Search our Catalog", and several GAL4 antibodies will pop up. I haven't used any of them, but they give journal references that have figures showing data obtained by using their antibodies. Good Luck, Alex Brands --- From owner-arab-gen@hgmp.mrc.ac.uk Sat Jun 10 00:16:48 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 77F8217A7F; Sat, 10 Jun 2000 00:16:45 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 1F31A17AE8; Sat, 10 Jun 2000 00:16:44 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: rhee@acoma.Stanford.EDU (Sue Rhee) Subject: TAIR curator positions Date: 9 Jun 2000 21:17:03 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk The Arabidopsis Information Resource (TAIR) is seeking two full-time database curators to start in the fall of 2000. They will participate in the collection and display of data for research use, the development and improvement of data model for TAIR database, and the development of software tools for literature curation, data integration, visualization, and analysis. Data types to curate and develop into the database structure include experimental data from the Arabidopsis literature, genome annotation, functional genomics, microarrays, phenotype and mutant lines, and biochemical pathways. Successful candidates will have a Ph.D degree or equivalent in biology, a demonstrated ability for independent, critical thinking and excellent communication and teamwork skills. Prior laboratory experience with Arabidopsis is preferred. Candidates with some background in biochemistry are especially encouraged to apply. Also, candidates with a working knowledge of PERL, SQL, and/or basic shell scripting languages is desired but an extensive background in computing is not required. Familiarity with basic UNIX commands, spreadsheets, and commonly used biological research tools such as BLAST and MEDLINE searching would be assets. For more information contact us by email at: curator@arabidopsis.org Qualified candidates should send a resume to: The Arabidopsis Information Resource Attn: Sue Rhee Carnegie Institution of Washington, Dept. of Plant Biology 260 Panama Road Stanford, CA 94305 APPLICATION DEADLINE: JULY 31, 2000 ----------------------------------------------------------------------------- Sue Rhee rhee@acoma.stanford.edu The Arabidopsis Information Resource URL: www.arabidopsis.org Carnegie Institution of Washington FAX: +1-650-325-6857 Department of Plant Biology Tel: +1-650-325-1521 ext. 251 260 Panama St. Stanford, CA 94305 U.S.A. ----------------------------------------------------------------------------- Please note that the AtDB project has officially ended and will go offline soon. It is replaced by TAIR (The Arabidopsis Information Resource). Please update your AtDB bookmarks to TAIR (www.arabidopsis.org) at your earliest convenience. ******************************************************************* --- From owner-arab-gen@hgmp.mrc.ac.uk Sat Jun 10 23:11:43 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 4440117AB5; Sat, 10 Jun 2000 23:11:40 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 2DC4417AB7; Sat, 10 Jun 2000 23:11:39 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: zheng@brookes.ac.uk ("Hugo Zheng") Subject: where to get antibodies for arabidopsis vacoule and ER? Date: 10 Jun 2000 02:30:28 +0100 Organization: Personal Station Message-ID: <000d01bfd27a$6e61a7c0$5a248cd4@default> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk It seems that some anti-TIPs (from tobacco, pea) do not work for arabidopsis sample, so just windering if anti-BiP (Bioreagent, CO) works or not in arabidopsis. Wondering who may know a good sources for labelling arabidopsis vacuole and ER that can be used in Western. Thanks. Hugo --- From owner-arab-gen@hgmp.mrc.ac.uk Sat Jun 10 23:12:03 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 192B517A7C; Sat, 10 Jun 2000 23:11:59 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 514B017ABA; Sat, 10 Jun 2000 23:11:57 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: jagreet@ccmb.ap.nic.in (Jagreet Kaur) Subject: anti-peptide antibody Date: 10 Jun 2000 17:22:13 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hi All Thanks a lot for the suggestions posted in reply to my query. Here are the replies thatI got: From: Jasmina Kurepa I was working on a protein which was also a member of a multigene family, so my only solution were the anti-peptide antibodies. Good news is that it worked really good. My basic steps were: 1. Prediction of hydrophylicity based on Hoop and Woods (PNAS USA 78; 3824-3828 from 1981). That was done with the program ANTIGEN which was a part of the PC/Gene swite (I guess there must be a stand-alone program somewhere on the web). 2. Prediction of chain flexibility based on Karplus and Schultz (Naturwissenshaften 72: 212-213 from 1985). That was cone bu the program FLEXPRO from PC/Gene. 3. Peptide was coupled to the kayhole limpet hemocyanin as in Mumby et al (PNAS USA 83: 265-269 from 1986). 4. I injected 3 rabbits and only one gave reasonably good antisera - I had to purify the IgGs and concentrate before it become good antisera. Parts of my ordeal are in Kurepa et al in Plant Physiology and Biochemistry 35; 467-474 (1997). From: Barbara Moffatt moffatt@sciborg.uwaterloo.ca Jagreet: I have had good success at this using the facilities of the Alberta Peptide Institute. They have proprietary software that analyzes the proteins and predicts the best peptides based on several criteria. They also make the peptides conjugated to KLH. I have always had success with the peptides they have designed and made. You can contact them at Dept of Biochemistry, University of Alberta, Fax 403-492-7168. The person I spoke with was Dr. Robert Parker. His phone number is 403-492-3907. He has email but I don't have it handy. Likely you can get it from the U of Alberta web page. Kind regards Barb Moffatt "Immink, R." R.Immink@plant.wag-ur.nl There is a company in Belgium (Eurogentec) which provides these kind of services. You can send your protein sequence and they will select the best domains for raising an antibody. You can select yourself one of these domains and let them make the antibody against it. You can have a look at their website for more info: http://www.eurogentec.be/ Good luck, From: Francois Ouellet ouelletf@EM.AGR.CA Here are a few points that you should look for. Most of the information comes from "Current Protocols in Protein Science" and "Antibodies", two very well known lab protocols books. The region to choose as an epitope should be like this: . of course, it has to be totally different from the other members of the family . 10 amino acids or longer; 12-15 residues long is good . better to have more than one peptide for the protein . better to have a couple of 12 a.a. peptides than only one of 25-30 a.a. . highly hydrophilic . highly mobile (containing glycine and proline residues) . best if it is at the C-terminus . you have to add a cysteine residue at the N-terminus if the peptide is to be coupled to the carrier using MBS * in this case, there should not be a cysteine residue within your peptide You can check the following web site to find programs to analyze your protein.This is the reference site for protein work. http://www.expasy.ch Another possibility would be to analyze your protein using the "peptidestructure" command on the GCG software package from the University of Wisconsin, if you have access to it. I had an antibody made against an internal peptide of a family of over 20 members. I was successful in generating an antibody against a peptide of 12amino acids (13 with the cysteine), coupled to the carrier KLH using MBS as acoupling agent. I had the antibody made at Genosys, a company in the USA. They did everything after I sent them the sequence of the peptide: peptide synthesis, coupling to KLH, injections in rabbits, blood collection. The antibody was excellent for western blot analysis but showed a very low efficiency in immunoprecipitation. You may want to use a few peptides if you are to do IP. The mixture of peptides can be injected in the same rabbit. Theonly thing is that the cost of peptide synthesis is prohibitive. I hope this will help. Cheers. Francois Francois Ouellet Agriculture and Agri-Food Canada From: Mike Mulligan rmmullig@uci.edu We have made several anti-peptide antibodies, take a look at these papers: rps12 Transcripts Results in the Synthesis of Polymorphic Polypeptides in Plant Mitochondria. The Plant Cell 8:107-117 Williams, M.A., Tallakson, W.A. Phreaner, C.G., and R.M. Mulligan (1998) Editing and Translation of Ribosomal Protein S13 Transcripts: Unedited Translation Products Are Not Detectable in Maize Mitochondria. Current Genetics 34:221-226 Let ne know if you have other questions. Mike From: Jon Monroe monroejd@jmu.edu I recently went through the same process that you are going through and found lots of good information at http://www.genosys.com/index2.html. They (Sigma Genosys) were quite helpful and I'm looking forward to getting the antibodies in a few weeks. Best of luck, Jon "Jerry D. Cohen" cohen047@tc.umn.edu We have done this fairly often through Research Genetics (http://www.resgen.com/). There are a lot of different things to consider,but they are good at walking you through the details. Of course you need to decide if you want to pick up the whole family or design a specific antibody to your family member, etc. Hope this helps, Jerry Dr. Jerry D. Cohen Professor, Bailey Endowed Chair Department of Horticultural Science University of Minnesota 1970 Folwell Ave., 305 Alderman Hall --- From owner-arab-gen@hgmp.mrc.ac.uk Sat Jun 10 23:12:20 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 03A4317B96; Sat, 10 Jun 2000 23:12:14 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 5D8E017B96; Sat, 10 Jun 2000 23:12:11 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: curator@arabidopsis.org Subject: New/Updated Arabidopsis DNA 10-Jun-2000 Date: 10 Jun 2000 21:21:50 +0100 Organization: The Arabidopsis Information Resource Message-ID: <200006102021.OAA27384@cleveland.ncgr.org> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Arabidopsis researchers, The following information is the number of Arabidopsis entries in GenBank that are new or were updated in the last week. These sequences have been updated in TAIR and are available for searching using TAIR BLAST and FASTA at: http://arabidopsis.org/blast/ http://arabidopsis.org/cgi-bin/fasta/TAIRfasta.pl New sequences : 48 Updated sequences : 0 Updated annotation : 23 ====================== New DNA Sequences ======================= ATH271731 AJ271731 1332bp RNA PLN 01-JUN-2000 Arabidopsis thaliana mRNA for branched-chain amino acid transaminase (bcat2 gene) bcat2 gene; branched-chain amino acid transaminase; bcat2. ATH271732 AJ271732 1286bp RNA PLN 01-JUN-2000 Arabidopsis thaliana mRNA for branched-chain amino acid transaminase (bcat4 gene) bcat4 gene; branched-chain amino acid transaminase; bcat4. ATH276123 AJ276123 1368bp RNA PLN 01-JUN-2000 Arabidopsis thaliana mRNA for branched-chain amino acid transaminase (bcat1 gene) bcat1 gene; branched-chain amino acid transaminase; bcat1. ATH276124 AJ276124 1539bp RNA PLN 01-JUN-2000 Arabidopsis thaliana mRNA for branched-chain amino acid transaminase 3 (bcat3 gene) bcat3 gene; branched-chain amino acid transaminase 3; bcat3. AF207994 AF207994 2304bp mRNA PLN 02-JUN-2000 Arabidopsis thaliana acyl-CoA oxidase ACX3 (ACX3) mRNA, complete cds. ACX3; acyl-CoA oxidase ACX3. AF223949 AF223949 2165bp mRNA PLN 02-JUN-2000 Arabidopsis thaliana amidase mRNA, complete cds. amidase. AF229850 AF229850 2972bp DNA PLN 02-JUN-2000 Arabidopsis thaliana ferritin (Fer1) gene, complete cds. Fer1; ferritin. AF236376 AF236376 542bp mRNA PLN 02-JUN-2000 Arabidopsis thaliana IPS1 mRNA, complete sequence. IPS1. AF239718 AF239718 4013bp DNA PLN 02-JUN-2000 Arabidopsis thaliana SGS2 gene, complete cds. SGS2. AF239719 AF239719 2254bp DNA PLN 02-JUN-2000 Arabidopsis thaliana SGS3 gene, complete cds. SGS3. AC069470 AC069470 291898bp DNA HTG 01-JUN-2000 Arabidopsis thaliana chromosome I clone IGF-F13E17, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces. HTG; HTGS_PHASE1. AC069471 AC069471 127042bp DNA HTG 01-JUN-2000 Arabidopsis thaliana chromosome I clone IGF-F13K9, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC069472 AC069472 52211bp DNA HTG 01-JUN-2000 Arabidopsis thaliana chromosome III clone BAC pool-F28J15, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC069473 AC069473 85822bp DNA HTG 01-JUN-2000 Arabidopsis thaliana chromosome III clone BAC pool-T21B14, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC069474 AC069474 106390bp DNA HTG 01-JUN-2000 Arabidopsis thaliana chromosome III clone BAC pool-T2E22, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. F214505S12 AF214500 1425bp DNA PLN 17-MAY-2000 Arabidopsis thaliana fiddlehead protein (fdh) gene, fdh-5 allele, complete cds. fdh; fiddlehead protein. F214505S03 AF214491 656bp DNA PLN 17-MAY-2000 Arabidopsis thaliana fiddlehead protein (fdh) gene, fdh-1 allele, exon sequence. fdh. F214505S14 AF214502 1425bp DNA PLN 17-MAY-2000 Arabidopsis thaliana fiddlehead protein (fdh) gene, fdh-6 allele, complete cds. fdh; fiddlehead protein. F214505S07 AF214495 656bp DNA PLN 17-MAY-2000 Arabidopsis thaliana fiddlehead protein (fdh) gene, fdh-3 allele, exon sequence. fdh. F214505S06 AF214494 1425bp DNA PLN 17-MAY-2000 Arabidopsis thaliana fiddlehead protein (fdh) gene, fdh-2 allele, complete cds. fdh; fiddlehead protein. F214505S09 AF214497 656bp DNA PLN 17-MAY-2000 Arabidopsis thaliana fiddlehead protein (fdh) gene, fdh-4 allele, exon sequence. fdh. F214505S17 AF214505 656bp DNA PLN 17-MAY-2000 Arabidopsis thaliana fiddlehead protein (fdh) gene, fdh-9 allele, exon sequence. fdh. F214505S01 AF214489 656bp DNA PLN 17-MAY-2000 Arabidopsis thaliana cultivar Landsberg erecta fiddlehead protein (fdh) gene, exon sequence. fdh. F214505S10 AF214498 1425bp DNA PLN 17-MAY-2000 Arabidopsis thaliana fiddlehead protein (fdh) gene, fdh-4 allele, complete cds. fdh; fiddlehead protein. F214505S11 AF214499 656bp DNA PLN 17-MAY-2000 Arabidopsis thaliana fiddlehead protein (fdh) gene, fdh-5 allele, exon sequence. fdh. F214505S02 AF214490 1425bp DNA PLN 17-MAY-2000 Arabidopsis thaliana cultivar Landsberg erecta fiddlehead protein (fdh) gene, complete cds. fdh; fiddlehead protein. F214505S08 AF214496 1425bp DNA PLN 17-MAY-2000 Arabidopsis thaliana fiddlehead protein (fdh) gene, fdh-3 allele, complete cds. fdh; fiddlehead protein. F214505S15 AF214503 656bp DNA PLN 17-MAY-2000 Arabidopsis thaliana fiddlehead protein (fdh) gene, fdh-7 allele, exon sequence. fdh. F214505S05 AF214493 656bp DNA PLN 17-MAY-2000 Arabidopsis thaliana fiddlehead protein (fdh) gene, fdh-2 allele, exon sequence. fdh. F214505S04 AF214492 1425bp DNA PLN 17-MAY-2000 Arabidopsis thaliana fiddlehead protein (fdh) gene, fdh-1 allele, complete cds. fdh; fiddlehead protein. F214505S13 AF214501 656bp DNA PLN 17-MAY-2000 Arabidopsis thaliana fiddlehead protein (fdh) gene, fdh-6 allele, exon sequence. fdh. F214505S16 AF214504 1425bp DNA PLN 17-MAY-2000 Arabidopsis thaliana fiddlehead protein (fdh) gene, fdh-7 allele, complete cds. fdh; fiddlehead protein. F214505S18 AF214506 1425bp DNA PLN 17-MAY-2000 Arabidopsis thaliana fiddlehead protein (fdh) gene, fdh-9 allele, complete cds. fdh; fiddlehead protein. AC069551 AC069551 92293bp DNA HTG 03-JUN-2000 Arabidopsis thaliana chromosome 1 clone T10O22, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces. HTG; HTGS_PHASE1. AC069552 AC069552 93452bp DNA HTG 03-JUN-2000 Arabidopsis thaliana chromosome 5 clone F17M07, *** SEQUENCING IN PROGRESS ***, 10 unordered pieces. HTG; HTGS_PHASE1. AC069553 AC069553 110861bp DNA HTG 03-JUN-2000 Arabidopsis thaliana chromosome 5 clone F18A12, *** SEQUENCING IN PROGRESS ***, 16 unordered pieces. HTG; HTGS_PHASE1. AC069554 AC069554 86537bp DNA HTG 03-JUN-2000 Arabidopsis thaliana chromosome 5 clone F19I11, *** SEQUENCING IN PROGRESS ***, 13 unordered pieces. HTG; HTGS_PHASE1. AC069555 AC069555 107272bp DNA HTG 03-JUN-2000 Arabidopsis thaliana chromosome 5 clone F7I20, *** SEQUENCING IN PROGRESS ***, 10 unordered pieces. HTG; HTGS_PHASE1. AC069556 AC069556 89547bp DNA HTG 03-JUN-2000 Arabidopsis thaliana chromosome 5 clone T1G16, *** SEQUENCING IN PROGRESS ***, 11 unordered pieces. HTG; HTGS_PHASE1. AC069557 AC069557 119935bp DNA HTG 03-JUN-2000 Arabidopsis thaliana chromosome 5 clone T29A4, *** SEQUENCING IN PROGRESS ***, 11 unordered pieces. HTG; HTGS_PHASE1. AC069558 AC069558 97859bp DNA HTG 03-JUN-2000 Arabidopsis thaliana chromosome 5 clone T5G13, *** SEQUENCING IN PROGRESS ***, 15 unordered pieces. HTG; HTGS_PHASE1. AF268093 AF268093 5280bp DNA PLN 05-JUN-2000 Arabidopsis thaliana RNA-dependent RNA polymerase (SDE1) gene, complete cds. RNA-dependent RNA polymerase; SDE1. AP002457 AP002457 24294bp DNA PLN 07-JUN-2000 Arabidopsis thaliana genomic DNA, chromosome 3, P1 clone:MWE13. HTG. AP002458 AP002458 67517bp DNA PLN 07-JUN-2000 Arabidopsis thaliana genomic DNA, chromosome 3, BAC clone:T22C2. HTG. AP002459 AP002459 30780bp DNA PLN 07-JUN-2000 Arabidopsis thaliana genomic DNA, chromosome 3, BAC clone:T13B17. HTG. AP002460 AP002460 111554bp DNA PLN 07-JUN-2000 Arabidopsis thaliana genomic DNA, chromosome 3, BAC clone:F1D9. HTG. AP002461 AP002461 10607bp DNA PLN 07-JUN-2000 Arabidopsis thaliana genomic DNA, chromosome 3, BAC clone:T22P15. HTG. AP002462 AP002462 36329bp DNA PLN 07-JUN-2000 Arabidopsis thaliana genomic DNA, chromosome 3, BAC clone:T8N9. HTG. =========== Updated Sequence Features/Annotations ============= AC000348 AC000348 111566bp DNA PLN 20-MAY-2000 Genomic sequence for Arabidopsis thaliana BAC T7N9 from chromosome I, complete sequence. HTG. AF110771 AF110771 1846bp mRNA PLN 05-JUN-2000 Arabidopsis thaliana ammonium transporter mRNA, complete cds. ammonium transporter. AF036300 AF036300 1359bp mRNA PLN 05-JUN-2000 Arabidopsis thaliana scarecrow-like 1 (SCL1) mRNA, partial cds. scarecrow-like 1; SCL1. AF036301 AF036301 1231bp mRNA PLN 05-JUN-2000 Arabidopsis thaliana scarecrow-like 3 (SCL3) mRNA, partial cds. scarecrow-like 3; SCL3. AF036302 AF036302 1065bp mRNA PLN 05-JUN-2000 Arabidopsis thaliana scarecrow-like 5 (SCL5) mRNA, partial cds. scarecrow-like 5; SCL5. AF036303 AF036303 1279bp mRNA PLN 05-JUN-2000 Arabidopsis thaliana scarecrow-like 6 (SCL6) mRNA, partial cds. scarecrow-like 6; SCL6. AF036304 AF036304 527bp mRNA PLN 05-JUN-2000 Arabidopsis thaliana scarecrow-like 7 (SCL7) mRNA, partial cds. scarecrow-like 7; SCL7. AF036305 AF036305 1900bp mRNA PLN 05-JUN-2000 Arabidopsis thaliana scarecrow-like 8 (SCL8) mRNA, partial cds. scarecrow-like 8; SCL8. AF036306 AF036306 726bp mRNA PLN 05-JUN-2000 Arabidopsis thaliana scarecrow-like 9 (SCL9) mRNA, partial cds. scarecrow-like 9; SCL9. AF036307 AF036307 760bp mRNA PLN 05-JUN-2000 Arabidopsis thaliana scarecrow-like 11 (SCL11) mRNA, partial cds. scarecrow-like 11; SCL11. AF036308 AF036308 1078bp mRNA PLN 05-JUN-2000 Arabidopsis thaliana scarecrow-like 13 (SCL13) mRNA, partial cds. scarecrow-like 13; SCL13. AF036309 AF036309 2635bp mRNA PLN 05-JUN-2000 Arabidopsis thaliana scarecrow-like 14 (SCL14) mRNA, partial cds. scarecrow-like 14; SCL14. AC011810 AC011810 103157bp DNA HTG 01-JUN-2000 Arabidopsis thaliana chromosome I BAC T6J4 genomic sequence, complete sequence. HTG. ATF8J2 AL132969 99814bp DNA PLN 19-APR-2000 Arabidopsis thaliana DNA chromosome 3, BAC clone F8J2 beta-galactosidase precursor-like protein; F8J2_10; Spot 3 protein and vacuolar sorting receptor homolog; F8J2_20; putative protein; F8J2_30; F8J2_40; monodehydroascorbate reductase (NADH)-like protein; F8J2_50; protein kinase-like; F8J2_60; F8J2_70; F8J2_80; F8J2_90; fructose bisphosphate aldolase-like protein; F8J2_100; nuclear envelope membrane protein-like; F8J2_110; F8J2_120; peroxiredoxin-like protein; F8J2_130; cytochrome P450-like protein; F8J2_140; F8J2_150; pyruvate kinase-like protein; F8J2_160; F8J2_170; F8J2_180; 60S ribosomal protein-like; F8J2_190; serine protein kinase-like; F8J2_200; late embryogenesis abundant protein-like; F8J2_210; F8J2_220; kinetochore-like protein; F8J2_230; F8J2_240. AF210731 AF210731 2130bp mRNA PLN 05-JUN-2000 Arabidopsis thaliana scarecrow-like 1 (SCL1) mRNA, complete cds. SCL1; scarecrow-like 1. AC027134 AC027134 102184bp DNA HTG 01-JUN-2000 Arabidopsis thaliana chromosome I clone F13B4, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces. HTG; HTGS_PHASE2. AC034257 AC034257 98950bp DNA PLN 01-JUN-2000 Arabidopsis thaliana chromosome I BAC F11A6 genomic sequence, complete sequence. HTG. AC068143 AC068143 36844bp DNA PLN 01-JUN-2000 Arabidopsis thaliana chromosome 1 BAC T2D23 sequence, complete sequence. HTG. AC068900 AC068900 96240bp DNA HTG 02-JUN-2000 Arabidopsis thaliana chromosome III clone IGF-F11A12, *** SEQUENCING IN PROGRESS ***, 17 unordered pieces. HTG; HTGS_PHASE1. AP002067 AP002067 84711bp DNA PLN 07-JUN-2000 Arabidopsis thaliana genomic DNA, chromosome 3, BAC clone: T7B9. HTG. AC069325 AC069325 85237bp DNA HTG 03-JUN-2000 Arabidopsis thaliana chromosome 5 clone T10F18, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces. HTG; HTGS_PHASE1. AC069327 AC069327 93846bp DNA HTG 03-JUN-2000 Arabidopsis thaliana chromosome 5 clone T26G08, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces. HTG; HTGS_PHASE1. AF263518 AF263518 10989bp DNA PLN 02-JUN-2000 Arabidopsis thaliana protein synthesis initiation factor 4G (EIF4G) gene, complete cds. protein synthesis initiation factor 4G; EIF4G. --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Jun 14 00:19:51 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 463C017ABA; Wed, 14 Jun 2000 00:19:48 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 10FF217B25; Wed, 14 Jun 2000 00:19:46 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: rhee@acoma.Stanford.EDU (Sue Rhee) Subject: Arabidopsis reprints Date: 13 Jun 2000 06:45:00 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Arabidopsis researchers, TAIR is gearing up to start curating experimental data from Arabidopsis literature this summer. As a first step, we would like to collect reprints on Arabidopsis research for internal use to curate the data into TAIR database. We would greatly appreciate it if you could send us your reprints to the address below. Alternatively we encourage you to bring them by to the TAIR drop-in station at the Arabidopsis meeting in Madison. All the best, ----------------------------------------------------------------------------- Sue Rhee rhee@acoma.stanford.edu The Arabidopsis Information Resource URL: www.arabidopsis.org Carnegie Institution of Washington FAX: +1-650-325-6857 Department of Plant Biology Tel: +1-650-325-1521 ext. 251 260 Panama St. Stanford, CA 94305 U.S.A. ----------------------------------------------------------------------------- Please note that the AtDB project has officially ended and will go offline soon. It is replaced by TAIR (The Arabidopsis Information Resource). Please update your AtDB bookmarks to TAIR (www.arabidopsis.org) at your earliest convenience. ******************************************************************* --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Jun 14 00:20:29 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id B67B217ABA; Wed, 14 Jun 2000 00:20:25 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 826B117B28; Wed, 14 Jun 2000 00:20:24 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: Candace_Waddell@maclan.mcgill.ca (Candace Waddell) Subject: Faculty Position in Plant Biology Date: 13 Jun 2000 16:17:58 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk The text below is a copy of an ad from the Department of Biology at McGill University. We welcome applications from researchers working in any area of plant molecular biology. Candace Waddell ************************************ McGill University Department of Biology Plant Molecular Biology The Department of Biology at McGill University invites applications for a tenure-track position at the Assistant, Associate or Full Professor level in the area of plant molecular biology. Applicants should have a Ph.D. degree and postdoctoral experience and have demonstrated research excellence. The successful applicant will join a large and dynamic department with research strengths in cellular, molecular and developmental biology, ecology, evolution and neurobiology, with extensive affiliations with the Faculty of Medicine and local research institutes. Additional information on the Department is available at: http://www.mcgill.ca/Biology/biology1.htm. Duties will involve the development of a vigorous research program and teaching at the undergraduate and graduate level. Applications, including a curriculum vitae, a statement of research interests, and three letters of reference should be sent to: Ms. Louise Sabaz, Chair's Secretary, Department of Biology, McGill University, 1205 Docteur Penfield Avenue, Montreal, Quebec H3A 1B1, Canada. . The deadline for applications is July 15, 2000. In accordance with Canadian immigration requirements, this advertisement is directed in the first instance to Canadian citizens and permanent residents. McGill University is committed to equity in employment. ---------------------------------------- Candace S. Waddell Dept. of Biology, McGill University 1205 Dr. Penfield Ave., Montreal Quebec, Canada H3A 1B1 phone: (514) 398-6451 FAX: (514) 398-5069 email: Candace_Waddell@maclan.mcgill.ca ---------------------------------------- --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Jun 14 00:21:00 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 4622817ABA; Wed, 14 Jun 2000 00:20:55 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 3400A17B2D; Wed, 14 Jun 2000 00:20:54 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: vierling@U.Arizona.EDU (Elizabeth Vierling) Subject: postdoctoral position Date: 13 Jun 2000 23:54:17 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <3.0.6.32.20000613155250.0083f100@pop.u.arizona.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk POSTDOCTORAL POSITION available immediately to study genetic mechanisms of adaptation to high temperature in Arabidopsis thaliana (see P.N.A.S. 97:4392, 2000). Candidate must have a PhD in biochemistry or related field, experience with Arabidopsis molecular and transmission genetics, and an interest in plant interactions with the environment. For further information see http://www.biochem.arizona.edu/vierling/. Send curriculum vitae, brief statement of research interests and have three letters of reference sent to: Dr. Elizabeth Vierling, Department of Biochemistry, The University of Arizona, Tucson, AZ 85721. E-mail: vierling@u.arizona.edu. Review of applicant materials will begin 6/28/00 and will continue until the position is filled. The University of Arizona is an Equal Employment Opportunity/Affirmative Action Employer. Elizabeth Vierling Biochemistry Department University of Arizona Life Sciences South 1007 E. Lowell St. Tucson, Arizona 85721 USA Phone: 520-621-1601 Fax: 520-621-3709 E-mail: vierling@u.arizona.edu --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Jun 15 03:08:24 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id E52ED17B8A; Thu, 15 Jun 2000 03:08:20 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id D3F5A17B8D; Thu, 15 Jun 2000 03:08:19 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: chenqian@igt.ac.cn (chen qian) Subject: Have you have Ti plasmid inserting bar Date: 14 Jun 2000 03:51:47 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <18449.000614@igt.ac.cn> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hi,everyone: I need a Ti plasmid inserted with bar,nptII and Lb and Rb. Would anyone give me a favour to send me such plasmid? Thank you very much! Best regards, Yours sincerely: Chen mailto:chenqian@igt.ac.cn --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Jun 15 03:09:30 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id A604C17B8A; Thu, 15 Jun 2000 03:09:26 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 2F13217B8E; Thu, 15 Jun 2000 03:09:25 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: chenqian@igt.ac.cn (chen qian) Subject: Where could I get Silwet L-77? Date: 14 Jun 2000 04:05:39 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <0658.000613@igt.ac.cn> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hi,Everyone: I am graduate student and I am doing work on Arabidopsis transformation. Now I want try the floral dipping transformation protocol published on the www.arabidopsis.org . Is there anyone who could tell me how I can get Silwet L-77? Thank you very much! Best regards, Yours sincerely: Chen mailto:chenqian@igt.ac.cn --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Jun 15 03:49:31 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 0BBD617B92; Thu, 15 Jun 2000 03:49:26 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 52C2517B93; Thu, 15 Jun 2000 03:49:25 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: torki@sakura.cc.tsukuba.ac.jp (Moez TORKI) Subject: cDNA Macroarrays ? Date: 14 Jun 2000 07:07:45 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear all, I am planning to study the expression of genes from tomato by macroarrays technology. I would like to counsel with you about : (1) what cDNA construction kit would most suitable for that purpose ? (Stratagene, Gibco, Clonetech...) ? (2) What would be the method of choice for library normalization ? (any published refernce would be fine) (3) What would be the membrane of choice for spotting the templates > (4) Is it needed to cnvert the library into plasmid library for the screening or would it be enough to perform PCR reactions from individual phage clones ? Thanks for taking the time to sharing your experience. I will summarize all relevant replies. Best Regards, Moez TORKI _________________________________________ Moez TORKI, Eng., Ph.D. University of Tsukuba, Institute of Agriculture and Forestry, Tsukuba, Ibaraki 305-8572, JAPAN +81 (0) 298 53 7262 / +81 (0) 298 53 4710 (phone) +81 (0) 298 53 6617 (fax) torki@sakura.cc.tsukuba.ac.jp (email) http://www.tunisia.or.jp/tiwss99 http://stafellow.jst.go.jp/life/today011/torki.html http://www.intio.or.jp/tsukubaj/news/0006/top.html _________________________________________ The World : Once thought to be Flat, then Proved to be Round. Now it's quite definitely Web Shaped.... --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Jun 15 03:50:42 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 2ADDE17B92; Thu, 15 Jun 2000 03:50:39 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 1D99B17B94; Thu, 15 Jun 2000 03:50:38 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: borock@ust.hk (Chris Rock) Subject: Postdoctoral position Date: 14 Jun 2000 08:23:40 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Postdoctoral Position Hong Kong Univ. Sci. & Technol. To investigate the molecular mechanisms of ABA signal transduction. We are chromosome walking to Arabidopsis mutants that ectopically express an ABA-inducible reporter and have pleiotropic effects on morphogenesis (Planta 210: 875 and abstract #232, http://www.wisc.edu/union/info/conf/arabidopsis/reginfo_arab.html). A second project involves flow cytometry, electrophysiology, and biochemistry of rice protoplasts that express ABA-inducible GFP. We are focusing on the role of a cell surface glycoprotein that may interact with an ABA receptor (FEBS Lett. 456: 257), and the mechanism of action of trivalent ions (Plant Physiol., in press). Salary is US$37,100 per annum plus benefits. Send email with research interests and experience, career goals, curriculum vitae, and names of 3 references to: Chris Rock, Dept. Biology, HKUST, Clear Water Bay, Kowloon, Hong Kong. Email: borock@ust.hk; http://www.ust.hk/~webbo/. --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Jun 15 03:51:47 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 1AD6717B92; Thu, 15 Jun 2000 03:51:43 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 51DCB17B95; Thu, 15 Jun 2000 03:51:41 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: meinke@okstate.edu (DAVID W MEINKE) Subject: Temporary Teaching Position Date: 14 Jun 2000 14:43:02 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Colleagues: A temporary (4.5 or 9-month) position is available in my department at Oklahoma State University in Stillwater to teach undergraduate courses (with labs) in plant anatomy (Fall 2000) and plant microtechniques (Spring 2001). Fall semester begins August 21, 2000. Applicants should have some teaching experience, familiarity with fundamental concepts in plant anatomy and microtechniques, and good communication skills. Individuals with research expertise in modern cell biology and the analysis of reproductive development in Arabidopsis are particularly encouraged to apply. Facilities are available for research collaborations with my laboratory during the period of appointment. Interested individuals may learn more about the position and department by contacting me by phone or email (see below) or in person at the Madison meeting. Applicants should send a letter of application, resume, and two letters of reference to: Dr. Becky Johnson, Head, Department of Botany, Oklahoma State University, Stillwater, OK 74078 (bjohnso@okstate.edu; phone: 405-744-5559; FAX 405-744-7074). Application materials should be received no later than July 14, 2000. Regards, David Meinke ----- David W. Meinke Department of Botany Oklahoma State University Stillwater, OK 74078 Phone: 405-744-6549 FAX: 405-744-7074 (Note change) Email: meinke@osuunx.ucc.okstate.edu WWW: http://mutant.lse.okstate.edu/ --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Jun 16 20:46:26 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id B465D17ACC; Fri, 16 Jun 2000 20:46:23 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id AA42D17AF3; Fri, 16 Jun 2000 20:46:22 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: vierling@U.Arizona.EDU (Elizabeth Vierling) Subject: postdoctoral position Date: 14 Jun 2000 00:36:38 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <3.0.6.32.20000613163512.008332e0@pop.u.arizona.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk POSTDOCTORAL POSITION available to study the molecular chaperone activity of small heat shock proteins. Project involves biochemical analyses of in vitro systems, genetic dissection of structure/function relationships in a cyanobacterium, and collaborations with X-ray crystallographers and NMR spectroscopists. Candidate must have a PhD in biochemistry or related field and excellent background in protein purification and physical characterization. Experience with microbial genetics and physiology a plus. For further information see http://www.biochem.arizona.edu/vierling/. Send curriculum vitae, brief statement of research interests, and have three letters of reference sent to: Dr. Elizabeth Vierling, Department of Biochemistry, The University of Arizona, Tucson, AZ 85721. E-mail: vierling@u.arizona.edu. Review of applicant materials will begin 6/28/00 and will continue until the position is filled. The University of Arizona is an Equal Employment Opportunity/Affirmative Action Employer. Elizabeth Vierling Biochemistry Department University of Arizona Life Sciences South 1007 E. Lowell St. Tucson, Arizona 85721 USA Phone: 520-621-1601 Fax: 520-621-3709 E-mail: vierling@u.arizona.edu --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Jun 16 20:48:41 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 462BF17ACC; Fri, 16 Jun 2000 20:48:38 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 33F6517AFF; Fri, 16 Jun 2000 20:48:37 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: xieqi@ima.org.sg (Qi Xie) Subject: Bleached Arabodopsis plant Date: 15 Jun 2000 07:09:47 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Colleagues, We found recently bleached Arabidopsis plants (especially in flower and silique development stages) in our growth room. We would like to know whether you found the same case in your work. Do you know why this happens? Please share the experience to resolve this problem. Thanks in advance! QI ________________________________________ Qi Xie, Ph.D Laboratory of Plant Cell Biology Institute of Molecular Agrobiology 1 Research Link The National University of Singapore Singapore 117604 Phone. (65)872 7481 : (65)872 7484 Fax. (65)872 7007 E-mail. xieqi@xena.ima.org.sg ________________________________________ --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Jun 16 20:49:20 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id DC31617ACC; Fri, 16 Jun 2000 20:49:16 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 6598217B00; Fri, 16 Jun 2000 20:49:15 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: afadiel@bioinfo.sickkids.on.ca (Ahmed Fadiel) Subject: chaos game Date: 15 Jun 2000 16:28:23 +0100 Organization: The Hospital for Sick Children Message-ID: <39491F01.FBEC2E36@bioinfo.sickkids.on.ca> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hi, Please, if any one knows how can I got a copy of the cgr (chaos game representative), that runs on windows or a web site that provide interface for this program. Also, if there are other programs that present the DNA sequence in chaos. Please, let me know. Ahmed --- From owner-arab-gen@hgmp.mrc.ac.uk Mon Jun 19 02:43:38 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 0CCE617AAE; Mon, 19 Jun 2000 02:43:32 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 1526017AD0; Mon, 19 Jun 2000 02:43:31 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: curator@arabidopsis.org Subject: New/Updated Arabidopsis DNA 17-Jun-2000 Date: 17 Jun 2000 23:20:36 +0100 Organization: The Arabidopsis Information Resource Message-ID: <200006172220.QAA06804@cleveland.ncgr.org> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Arabidopsis researchers, The following information is the number of Arabidopsis entries in GenBank that are new or were updated in the last week. These sequences have been updated in TAIR and are available for searching using TAIR BLAST and FASTA at: http://arabidopsis.org/blast/ http://arabidopsis.org/cgi-bin/fasta/TAIRfasta.pl New sequences : 51843 Updated sequences : 0 Updated annotation : 16 =========== Updated Sequence Features/Annotations ============= ATAJ5130 AJ005130 1418bp DNA PLN 13-JUN-2000 Arabidopsis thaliana Sbe2.2 gene, 5' region. Sbe2.2 gene; Sbe2.2. AF019748 AF019748 1702bp mRNA PLN 08-JUN-2000 Arabidopsis thaliana high-affinity nitrate transporter ACH1 (ACH1) mRNA, complete cds. ACH1; high-affinity nitrate transporter ACH1. AF019749 AF019749 1709bp mRNA PLN 08-JUN-2000 Arabidopsis thaliana high-affinity nitrate transporter ACH2 (ACH2) mRNA, complete cds. ACH2; high-affinity nitrate transporter ACH2. AF029980 AF029980 1352bp mRNA PLN 08-JUN-2000 Arabidopsis thaliana A37 (A37) mRNA, complete cds. A37. AF029981 AF029981 3353bp DNA PLN 08-JUN-2000 Arabidopsis thaliana A37 (A37) gene, complete cds. A37. AB016570 AB016570 3260bp DNA PLN 09-JUN-2000 Arabidopsis thaliana DREB2A gene, complete cds. DREB2A. AB016571 AB016571 3646bp DNA PLN 09-JUN-2000 Arabidopsis thaliana DREB2B gene, complete cds. DREB2B. AC007183 AC007183 72236bp DNA HTG 08-JUN-2000 Arabidopsis thaliana chromosome 1 clone F9D18, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces. HTG; HTGS_PHASE1. ATH242988 AJ242988 3705bp DNA PLN 13-JUN-2000 Arabidopsis thaliana DNA for regulatory sequence which mediates guard cell specific gene expression promoter. AF056190 AF056190 1619bp mRNA PLN 06-JUN-2000 Arabidopsis thaliana Na+/H+ exchanger (NHX1) mRNA, complete cds. NHX1; Na+/H+ exchanger. AC021044 AC021044 121720bp DNA PLN 08-JUN-2000 Arabidopsis thaliana chromosome I BAC F3H9 genomic sequence, complete sequence. HTG. AC026757 AC026757 12357bp DNA HTG 07-JUN-2000 Arabidopsis thaliana chromosome I clone IGF-F2H10, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces. HTG; HTGS_PHASE1. AF230737 AF230737 909bp mRNA PLN 07-JUN-2000 Arabidopsis thaliana 4-Diphosphocytidyl-2C-methyl-D-erythritol synthase (ISPD) mRNA, complete cds. 4-Diphosphocytidyl-2C-methyl-D-erythritol synthase; ISPD. AC068655 AC068655 89453bp DNA PLN 06-JUN-2000 Genomic Sequence For Arabidopsis thaliana Clone F17K4 From Chromosome V, complete sequence. HTG. AC069143 AC069143 56438bp DNA PLN 06-JUN-2000 Arabidopsis thaliana chromosome 1 BAC T29M8 sequence, complete sequence. HTG. AC069144 AC069144 123133bp DNA HTG 07-JUN-2000 Arabidopsis thaliana chromosome I clone IGF-F14C21, *** SEQUENCING IN PROGRESS ***, 10 unordered pieces. HTG; HTGS_PHASE1. --- From owner-arab-gen@hgmp.mrc.ac.uk Mon Jun 19 02:44:30 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id AC3D717AAE; Mon, 19 Jun 2000 02:44:23 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 2F57717AD4; Mon, 19 Jun 2000 02:44:22 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: cherry@genome.stanford.edu (Mike Cherry) Subject: Arabidopsis cDNA Microarray Results Date: 18 Jun 2000 08:37:34 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <200006180737.AAA07488@fasolt.Stanford.EDU> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk The Arabidopsis Functional Genomics Consortium (AFGC) in collaboration with the Stanford Microarray Database (SMD) announces the public availability of AFGC microarray results. The SMD URL is: http://genome-www.stanford.edu/microarray Links to the SMD site can also be found at the AFGC Web site: http://afgc.stanford.edu/ and at the Stanford Genomics Resource site: http://genome-www.stanford.edu/ Arabidopsis microarray results can be located within SMD by selecting the "Public Search" button. The form that is displayed allows the user to access all currently public microarray data. As of today the results from 38 arrays (ie. slides) are available. Not all features of the Web site are yet complete. This is an on going effort. There will be several improvements over the coming months. Please see the HELP pages for information about the options on each page. For those attending the Arabidopsis Meeting in Madison there will be two workshops on microarrays. One will be presented by the AFGC and include descriptions of basic features provided by SMD. Several members of the AFGC bioinformatic team will be at Madison and will be staffing demonstrations of the resource throughout the meeting. The AFGC is funded by the NSF. SMD is a project database funded by the NCI/NIH. Mike J. Michael Cherry Internet: cherry@stanford.edu Department of Genetics Stanford University School of Medicine Medical Center, Room M341 Stanford, California 94305-5120 Voice: 650-723-7541 FAX: 650-723-7016 --- From owner-arab-gen@hgmp.mrc.ac.uk Mon Jun 19 23:48:24 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 342FB17A74; Mon, 19 Jun 2000 23:48:21 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 1909717AE0; Mon, 19 Jun 2000 23:48:19 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: MASLE@rsbs.anu.edu.au ("Josette Masle") Subject: Post-doctoral position in plant genomics Date: 19 Jun 2000 07:51:33 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk THE AUSTRALIAN NATIONAL UNIVERSITY INSTITUTE OF ADVANCED STUDIES RESEARCH SCHOOL OF BIOLOGICAL SCIENCES ENVIRONMENTAL BIOLOGY Postdoctoral Fellow in Root stress genomics Postdoctoral Fellow (Level A-step 6; fixed term) - $46,514 pa Ref: RSBS 2.2.1 A post-doctoral fellowship is available at The Australian National University, Research School of Biological Sciences to work in the area of root stress functional genomics. The successful candidate will join a research program aimed at understanding plant responses to edaphic stress, more specifically to root mechanical stress. He/She will be one of three appointees to a new genomic program initiated to investigate the molecular controls of these responses, using cereals and Arabidopsis as model systems. We are seeking a molecular biologist with experience in library construction and in state of the art molecular techniques such as RT-PCR and cDNA analysis by differential display and/or micro-array techniques. Experience in proteomics would also be very relevant. The appointee will have a central role in developing molecular approaches and techniques appropriate to the program and coordinating the work within the program. This will involve day to day supervision of a Technical Officer newly appointed to the project and close interactions with other members of the lab and with collaborators within the School. The work will also require coordination with several overseas labs, members of a genomic consortium collaborating on the project. Appointment: 2 or 3 year contracts with the possibility of a further contract, subject to funding. Starting date negotiable. Enquiries: Dr Josette Masle, tel: 61 2 6249 4410; e-mail: masle@rsbs.anu.edu.au Contact: Further particulars, including selection criteria must be obtained from Susan Toscan, Administration, RSBS, tel: (02) 6249 4752, fax: (02) 6249 4891, e-mail: Closing date: 21 July 2000 Information on how to apply may be obtained from the ANU Web page - http://www.anu.edu.au/hr/jobs or by telephoning/emailing the contact. All applications should be addressed to the Staffing Recruitment Officer, Chancelry 2A, Australian National University, CANBERRA ACT 0200. Applications from suitably qualified women are particularly welcome. Dr Josette Masle Environmental Biology Group RSBS- ANU GPO Box 475 Canberra ACT 2601 Australia Tel: 61 2 6249 4410 Fax: 61 2 6249 4919 --- From owner-arab-gen@hgmp.mrc.ac.uk Mon Jun 19 23:49:06 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 36CB117A74; Mon, 19 Jun 2000 23:49:00 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id F36C517AE9; Mon, 19 Jun 2000 23:48:57 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: Peter Rice Subject: Re: chaos game Date: 19 Jun 2000 09:18:13 +0100 Organization: The Sanger Centre Message-ID: References: <39491F01.FBEC2E36@bioinfo.sickkids.on.ca> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk afadiel@bioinfo.sickkids.on.ca (Ahmed Fadiel) writes: > Please, if any one knows how can I got a copy of the cgr (chaos game > representative), that runs on windows or a web site that provide > interface for this program. > Also, if there are other programs that present the DNA sequence in > chaos. The chaos program in EMBOSS does this. The EMBOSS home page is at http://www.sanger.ac.uk/Software/EMBOSS/ and the FTP distribution is at ftp://ftp.sanger.ac.uk/pub/EMBOSS/ You can also run chaos in EMBOSS at the Institut Pasteur web site: http://bioweb.pasteur.fr/seqanal/interfaces/chaos.html -- ---------------------------------------------------------------------- Peter Rice | Informatics Division, The Sanger Centre, E-mail: pmr@sanger.ac.uk | Wellcome Trust Genome Campus, Tel: (44) 1223 494967 | Hinxton, Cambridge, CB10 1SA, England Fax: (44) 1223 494919 | URL: http://www.sanger.ac.uk/Users/pmr/ From owner-arab-gen@hgmp.mrc.ac.uk Mon Jun 19 23:50:14 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 6F39817A74; Mon, 19 Jun 2000 23:50:10 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 3DC6517AEE; Mon, 19 Jun 2000 23:50:09 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: javierr@eden.rutgers.edu (Javier) Subject: Request help for a new research Date: 19 Jun 2000 16:30:41 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <394E3C96.188398E1@eden.rutgers.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear Dr. Rhee; My name is Javier Ramirez, PhD student at Rutgers Env. Science Dpt. I want to include the following test seed germination and plant growth of Arabidopsis using compost and lechate. the compost produced as amendment of the soil and the lechate of the compost as hydrophonic medium. In order to do that I have some questions for you: 1. to test arabidopsis on compost -Do I have to sterilize? the compost -What kind of soil I may use as a control - What materials I may require -What would be the procedure to do this test, in terms of temperature and nutrients 2. to test arabidopsis on lechate -Do I have to sterilize the lechate -What kind of solution I may use as control - What would be the procedure on this test - What materials I may require -What would be the procedure to do this test, in terms of temperature and nutrients I would like to understand the procedure first with the control and then I would follow with the samples. I understand that this is my job but you probably know more about that and you might refer to me to other source where I can get the answers. Thank you very much for your cooperation Javier --- From owner-arab-gen@hgmp.mrc.ac.uk Mon Jun 19 23:50:58 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 0ED6017AF2; Mon, 19 Jun 2000 23:50:53 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id B777617A74; Mon, 19 Jun 2000 23:50:52 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: finkel@genome.stanford.edu (David Finkelstein) Subject: Public Arabidopsis cDNA Microarray Results Date: 19 Jun 2000 16:32:16 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: References: <200006180737.AAA07488@fasolt.Stanford.EDU> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hi All, I've just added two new CO2 experiments to the public access portion of the data base. They are reverse duplicates, meaning they were probed with the same 2 RNAs. "Reverse" means the dye used for the treated RNA in the first chip was used for the control in the second and vice versa. It's a way to see any effects due solely to the chemistry of the dyes. I'll be also be talking about data, normalization, replication and interpretation of microarray results in WI next Sunday the 25th at the Arabidopsis meeting. We'll also have AFGC drop in sessions during the meeting. Naturally, your all invited. Regards, David Finkelstein NSF Post Doc.Fellow, Bioinformatics Department Plant Biology, Carnegie Institute of Washington 260 Panama Street Stanford, CA 94305 --- From owner-arab-gen@hgmp.mrc.ac.uk Mon Jun 19 23:51:24 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id A1C4E17AF2; Mon, 19 Jun 2000 23:51:20 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id A31AE17AF3; Mon, 19 Jun 2000 23:51:19 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: finkel@genome.stanford.edu (David Finkelstein) Subject: CO2 Experiments at SMD Date: 19 Jun 2000 16:44:12 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: References: <200006180737.AAA07488@fasolt.Stanford.EDU> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hi All, You can best access my experiments at: http://genome-www.stanford.edu/microarray Select the "Public Search" button. Then you'll get a new page select FINKEL and you'll be able to see 3 CO2 experiments. Even if CO2 doesn't interest you heres a chance to get a feel for the way our database functions. I should note that CO2 experiments are notoriously nitrogen dependent and the nitrogen status of the plants which yielded the RNA used in these chips has yet to be determined. Also CO2 experiments will be posted two other independent labs. Eventually the total data set will give all microarray users clear picture of the overall variability of plant microarray experiments. Regards, David Finkelstein NSF post doc fellow, Bioinformatics --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Jun 21 17:10:02 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 37B3A17AC3; Wed, 21 Jun 2000 17:09:59 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id D0D7F17AC7; Wed, 21 Jun 2000 17:09:57 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: meier.56@osu.edu (Iris Meier) Subject: Postdoctoral Position Date: 21 Jun 2000 01:40:36 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk A postdoctoral position is available to study the role of the plant nuclear envelope in chromatin organization and gene expression. Association of chromatin with the nuclear envelope has been recently connected with gene silencing and heterochromatin formation in animals and yeast. So far, no orthologs of the animal inner nuclear envelope proteins implied in chromatin attachment appear to be present in the Arabidopsis genome. We have identified two novel, potentially plant-specific proteins (MFP1 and MAF1) which are located at the nuclear envelope. MFP1 is a filament-like protein and binds in a phosphorylation-dependent way to matrix attachment region DNA. It is associated with the nuclear envelope via a novel targeting domain. MAF1 is a novel protein, which binds in yeast and in vitro to the coiled-coil domain of MFP1. Our hypothesis is that MFP1 is involved in attaching chromatin to the plant nuclear envelope and that MAF1 is involved in this process through interaction with MFP1. Potential projects for the postdoctoral researcher are the investigation of changes in nuclear architecture and gene expression pattern in knock-out mutants of MFP1 and MAF1 in Arabidopsis; the use of MFP1 and MAF1 as plant nuclear envelope markers during cell cycle using live dual imaging of GFP fusions; and the cell cycle-dependent regulation of MAR-binding by phosphorylation. For further information see: Meier et al. (1996), Plant Cell 8:2105; Gindullis and Meier (1999), Plant Cell 11:1117; Gindullis et al. (1999), Plant Cell 11:1755; Harder et al. (2000) Plant Phys. 122:225, and our webpage. To apply, please send a CV, a brief statement of research experience and research interests, and the names of at least three references (including address, phone, and email) to Iris Meier by mail or email. Successful postdoctoral candidates will have a Ph.D. and a proven record of productivity including first-authored publications in internationally recognized journals. Experience in either fluorescence microscopy or Arabidopsis molecular genetics will be a plus. If you are planning to attend the Arabidopsis meeting in Madison, please send me an email to arrange for a meeting, or meet us at posters 281 and 288. Iris Meier Plant Biotechnology Center Ohio State University 210 Rightmire Hall 1060 Carmack Road Columbus, OH 43210 PHONE: (614) 292 8323 FAX: (614) 292 5379 email: meier.56@osu.edu http://www.biosci.ohio-state.edu/~plantbio/plantbio.html --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Jun 21 17:11:15 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 4538417B17; Wed, 21 Jun 2000 17:11:11 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id B723617AC3; Wed, 21 Jun 2000 17:11:07 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: chenqianchina@yahoo.com (qian chen) Subject: Have you such plasmid? Date: 21 Jun 2000 06:42:07 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <20000621054202.4226.qmail@web5304.mail.yahoo.com> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hi,everyone: I a graduate student majoring in Plant molecular biology. Now I need a mini-Ti plasmid for my research work. Npt-II,bar,Lb, Rb and multicloning sites are necessary for my transformation work. If you have such plasmid, could you give me a favor to send me it? Thank you very much. Best regards Yours Sincerey:chen,qian Group603,Institute of Genetics Chinese Academy of Sciences Beijing,P.R.china, 100101 email address: chenqian@igt.ac.cn __________________________________________________ Do You Yahoo!? Send instant messages with Yahoo! Messenger. http://im.yahoo.com/ --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Jun 22 19:58:20 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 8B29D17A65; Thu, 22 Jun 2000 19:58:17 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 6CA5217A70; Thu, 22 Jun 2000 19:58:16 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: pietro.piffanelli@bbsrc.ac.uk (Pietro Piffanelli) Subject: Postdoctoral and Research assistant Positions - Sainsbury Date: 21 Jun 2000 19:00:38 +0100 Organization: J. Innes Centre Message-ID: <39510290.DFE88CB1@bbsrc.ac.uk> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk SAINSBURY LABORATORY John Innes Centre Postdoctoral and Research Assistant Positions Applications are invited for four, two year positions within the Sainsbury Laboratory. Two Postdoctoral Research Scientists and two Research Assistants are required to work on an exiting research project, currently funded by the Gatsby Foundation and Zeneca Ltd. This pioneering project will employ recently developed tools and utilize novel methodology to understand functions of genes required for disease resistance and cell death in plants (see Cell 1999, 99:355-366). Candidates should be highly motivated individuals with a PhD (for Postdoctoral positions) or a BSc, (for Research Assistant positions) with a background in biochemistry, genetics, molecular biology or a closely related field. Informal inquiries are welcome, please contact Dr Ken Shirasu ken.shirasu@bbsrc.ac.uk Salary on appointment for the Postdoctoral Research Scientist positions (Ref. Number 24 08 57/8) will be within the RA1A scale #16,286 to #24,479. Salary for the Research Assistant positions (Ref. Number 24 08 56/9) will be within the RA1B scale #16,286 to #18,185 per annum depending on qualifications and experience. To apply please send a full CV together with the name of two referees to the Personnel Officer, John Innes Centre, Colney, Norwich, NR4 7UH quoting the appropriate Reference No. The closing date for applications is 21 July 2000. --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Jun 22 19:59:04 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id D90A917A65; Thu, 22 Jun 2000 19:58:59 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 052E917A8C; Thu, 22 Jun 2000 19:58:56 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: tj@socrates.berkeley.edu (Tom Juenger) Subject: fully expanded leaves Date: 21 Jun 2000 20:46:18 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <4.1.20000621125133.009252e0@socrates.berkeley.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Hi; I'd like to make some measurements on Arabidopsis leaf morphology using mutants and ecotypes - most of the leaf molecular genetics papers make these sorts of measurements on "fully expanded" leaves, but they never really describe how they determine when a leaf is fully expanded. I know this sounds like a naive question, but does anyone have suggestions on how to determine when leaves are fully expanded, without making repeated measurements. Thanks TJ --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Jun 22 19:59:47 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id E7C6E17A94; Thu, 22 Jun 2000 19:59:42 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 8EA5517A65; Thu, 22 Jun 2000 19:59:41 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: ms73r@udcf.gla.ac.uk ("M.Shenton") Subject: Where can I get a Binary vector with unique SphI site? Date: 22 Jun 2000 10:08:42 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <3951D7FB.C2E@udcf.gla.ac.uk> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Could anyone tell me where I could get a small (ideally <10Kb) binary vector with a single SphI cloning site for inserting genomic DNA with a view to complementation. I've already spent quite some time looking through websites and catalogues. Thanks Bobby Brown Gareth Jenkins' Lab Glasgow email: rab3j@udcf.gla.ac.uk --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Jun 22 20:02:01 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 5B7C517AAC; Thu, 22 Jun 2000 20:01:58 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 1385817AD2; Thu, 22 Jun 2000 20:01:56 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: sean_may@thale.life.nottingham.ac.uk ("Sean May [ NASC ]") Subject: New 'sequence tagged insert' Blast server at NASC / AGR Date: 22 Jun 2000 15:53:45 +0100 Organization: ACS, The University of Nottingham Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Sequences derived from regions flanking transposon insertions (colloquially referred to as 'sequence tagged inserts' or 'insert sequences') are powerful tools for reverse genetics. Simple searches of insert sequences against the sequence of your gene can provide you with a potential mutant line, possibly a gene-function 'knockout'. Inspection of the sequence will also indicate where the insertion sits within the gene itself (e.g promoter; intron; exon; 3'UTR). This is the 'next step on' from PCR pool-hybridisation approaches for reverse genetics and will become increasingly useful as the available data on inserts expands to cover more of the genome / transcriptome. A second use for sequenced inserts in this post-genomic phase of Arabidopsis research is to provide 'launchpads' for local transposon saturation of the area around the insertion. This is possible from inspection of the 'Blast' derived genome position for the insert and the transient re-introduction of a transposase source. The NASC website now allows you to 'Blast' search against all public Arabidopsis sequence tagged inserts. The results of your search are then linked directly into the NASC stock catalogue to facilitate easy ordering of your chosen potential knockout(s). See: http://arabidopsis.org.uk/blast.html Currently you can search against SINS (Sainsbury Laboratory, Jonathan Jones' group, UK) and ITS (CPRO, Pereira Lab, Holland) sequences. In the near future we will also include sequence information from the Launchpad lines (John Innes, Paul Muskett, UK) and the IMA lines (IMA, Venkatesan lab, Singapore). Germplasm is already available at NASC for the SINS and IMAs and will shortly be available for the Launchpad lines (also to be available at ABRC) and Pereira insert lines. In addition you can keyword search pre-computed blast results of all SINS, IMA and ITS sequences searched against EMBL. This will find inserts based on a variety of keywords including specific genes, BACS, gene families or putative functions. The Arabidopsis Genome Resource (AGR - http://ukcrop.net/agr/) now contains improved information concerning insert sequences (IMA, SINS, and Pereira ITS) and provides direct links to our resources for searching insert homology information at NASC as described above. Please see http://ukcrop.net/agr/insert.html for more details. AGR provides positional information about the local genome surrounding the insertion of choice, and displays the insert position graphically in relation to the gene structure. AGR will contain new and updated insert information as soon as it becomes available and is actively developed as a genome / map-oriented complementary resource to the NASC germplasm catalogue. Please address enquiries as below: NASC catalogue: jamie@nasc.nott.ac.uk AGR / genomics: keith@nasc.nott.ac.uk Germplasm : karen@nasc.nott.ac.uk General: arabidopsis@nasc.nott.ac.uk or sean_may@nasc.nott.ac.uk All enquiries, donations of germplasm or 'insert sequences' are welcome. If you are aware of tagged sequence insertions that could be made available to the Arabidopsis community, please do contact sean_may@nasc.nott.ac.uk to explore how we might improve this resource. -------------------------------------------------------- Sean_May@nasc.nott.ac.uk Director, Nottingham Arabidopsis Stock Centre (NASC) Biological Sciences building, University Park, Nottingham University. U.K. NG7 2RD. FON: +44 (0)115 9513237 FAX: +44 (0)115 9513297 Catalogue: seeds.arabidopsis.org.uk Genomics: ukcrop.net Transcriptomics: garnet.arabidopsis.org.uk ICQ: 57288800 --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Jun 22 20:47:41 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id BC7D217AA7; Thu, 22 Jun 2000 20:47:37 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id B5DD917AD5; Thu, 22 Jun 2000 20:47:35 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: tbaum@iastate.edu (Thomas J Baum) Subject: Six Postdoc Positions in Molecular Plant Nematology Date: 22 Jun 2000 20:11:59 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <4.2.0.58.20000622140726.00a62950@tbaum.mail.iastate.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Postdoctoral Positions studying NEMATODE PARASITISM GENES The research labs of Dr. Thomas Baum at Iowa State University, Dr. Eric Davis at North Carolina State University, and Dr. Dick Hussey at the University of Georgia collaborate on a research project on the molecular basis of nematode parasitism of plants. Specifically we are studying parasitism genes expressed in the esophageal gland cells of the soybean cyst nematode, Heterodera glycines. We have adopted a functional genomics approach to study nematode parasitism genes, including the development of technologies for gland cell-specific cDNA library construction, cDNA libraries of nematode parasitic stages, genomic libraries, mRNA in situ hybridization within plant-parasitic nematodes, RNA interference, combinatorial peptide library screening, functional analyses in transformed plant tissues, and DNA microchips for nematode gene expression analyses. We currently are undertaking a large scale EST project of gland cell-specific cDNA libraries for identification of parasitism genes. Collectively, we have 6 postdoctoral positions (two in each lab) available for creative and energetic individuals to conduct novel research on nematode parasitism genes. Candidates should have a Ph.D. with a strong background in molecular biology, genetics, plant-microbe interactions, computational biology, or other techniques related to the project described above. Previous experience with plant-parasitic nematodes is not necessary. Salaries are commensurate with experience. Interested candidates should electronically submit a one-page statement of their interest and aptitude for the position, a 2-page CV, and contact information for three professional references. Information from applicants should be pasted into an e-mail (not attached) and sent to Drs. Baum (tbaum@iastate.edu), Davis (eric_davis@ncsu.edu), and/or Hussey (hussey@arches.uga.edu). **************************************************************************** Thomas J. Baum Assistant Professor Iowa State University Department of Plant Pathology 351 Bessey Hall Ames, IA 50011 USA ph. (515) 294-2398 fax (515) 294-9420 e-mail tbaum@iastate.edu http://www.public.iastate.edu/~trmaier/baumlab/baumfr.html ***************************************************************************** --- From owner-arab-gen@hgmp.mrc.ac.uk Thu Jun 22 20:48:22 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 7DA5C17ADE; Thu, 22 Jun 2000 20:48:16 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 2F7B117AA7; Thu, 22 Jun 2000 20:48:13 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: Mary.Lou.Guerinot@Dartmouth.EDU (Mary Lou Guerinot) Subject: postdoctoral position Date: 22 Jun 2000 20:33:21 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <37694183@donner.Dartmouth.EDU> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk POSTDOCTORAL POSITION Dartmouth College A postdoctoral research position is available immediately to study metal uptake in Arabidopsis. Our long term goal is to manipulate plant mineral status to create food crops with enhanced mineral content and to develop crops that can bioaccumulate or exclude toxic metals. We have identified a novel family of metal transporters from a diverse array of eukaryotes [see: Eide et al. 1996. Proc. Natl. Acad. Sci. U.S.A. 93: 5624; Grotz et al. 1998. Proc. Natl. Acad. Sci. U.S.A. 95: 7220]. Arabidopsis family members variously transport iron, zinc, manganese and cadmium when expressed in yeast. We have also identified the gene encoding ferric reductase (Robinson et al. 1999. Nature 397:694). This enzyme is required by most plants to acquire soluble iron. Projects currently underway include 1) a structure/function analysis of the proteins encoded by the ZIP gene family to identify regions of the protein responsible for substrate specificity and affinity, 2) analysis of ZIP gene expression and localization of ZIP proteins, 3) analysis of transgenic plants engineered to overexpress members of the ZIP gene family as well as lines carrying knockouts of various transporters for alterations in metal accumulation and 4) analysis of the role of various members of the ferric reductase gene family in metal metabolism. A background in molecular biology and genetics is required. If interested, please contact Mary Lou Guerinot at the Wisconsin Arabidopsis meeting. Alternatively, send a letter of application, Curriculum vitae, and names, addresses and FAX numbers of 3 references to Dr. Mary Lou Guerinot, Department of Biological Sciences, Dartmouth College, 6044 Gilman Lab, Hanover, NH 03755-3576. Telephone (603) 646-2527; FAX (603) 646-1347. E-mail inquiries are also welcome (guerinot@Dartmouth.edu). Dartmouth College is an equal opportunity/affirmative action employer. A few words about Dartmouth: Dartmouth is a small, Ivy League university with an undergraduate body of about 4300 and a graduate and professional school enrollment of approximately 850. We offer Ph.D programs in all of the Sciences; there is an extensive group whose research interests are focused on cellular, molecular and developmental biology (see http://www.dartmouth.edu/artsci/biochem/mcb/). We are situated in Hanover, a traditional New England town in the heart of winter skiing and summer lake resort areas. We are only a two hour drive from Boston and a three hour drive from Montreal, providing ample opportunities for sampling big city life. --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Jun 23 19:48:25 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id BE8F117A5D; Fri, 23 Jun 2000 19:48:22 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id AA65E17AAF; Fri, 23 Jun 2000 19:48:20 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: sean_may@thale.life.nottingham.ac.uk ("Sean May [ NASC ]") Subject: Re: Where could I get Silwet L-77? Date: 22 Jun 2000 21:44:15 +0100 Organization: ACS, The University of Nottingham Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Please try: arabidopsis.com (Lehle seeds) All the best, Sean -------------------------------------------------------- Sean_May@nasc.nott.ac.uk Director, Nottingham Arabidopsis Stock Centre (NASC) Biological Sciences building, University Park, Nottingham University. U.K. NG7 2RD. FON: +44 (0)115 9513237 FAX: +44 (0)115 9513297 Catalogue: seeds.arabidopsis.org.uk Genomics: ukcrop.net Transcriptomics: garnet.arabidopsis.org.uk ICQ: 57288800 --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Jun 23 19:48:59 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id C5D2017A5D; Fri, 23 Jun 2000 19:48:52 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id B716F17AB1; Fri, 23 Jun 2000 19:48:50 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: sean_may@thale.life.nottingham.ac.uk ("Sean May [ NASC ]") Subject: Re: Have you such plasmid? Date: 22 Jun 2000 21:46:50 +0100 Organization: ACS, The University of Nottingham Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Please try : http://www.pgreen.ac.uk/ All the best, Sean -------------------------------------------------------- Sean_May@nasc.nott.ac.uk Director, Nottingham Arabidopsis Stock Centre (NASC) Biological Sciences building, University Park, Nottingham University. U.K. NG7 2RD. FON: +44 (0)115 9513237 FAX: +44 (0)115 9513297 Catalogue: seeds.arabidopsis.org.uk Genomics: ukcrop.net Transcriptomics: garnet.arabidopsis.org.uk ICQ: 57288800 --- From owner-arab-gen@hgmp.mrc.ac.uk Fri Jun 23 19:49:36 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 3109117A5D; Fri, 23 Jun 2000 19:49:33 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id C0F2617AB6; Fri, 23 Jun 2000 19:49:31 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: zl17@umail.umd.edu (Zhongchi Liu) Subject: POSTDOCTORAL POSITION Date: 23 Jun 2000 00:19:47 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk A POSTDOCTORAL POSITION is available immediately to study the role of TSO1 in Arabidopsis inflorescence meristem development (Song et al., Development 127, 2207-2217, 2000; Liu et al., Development 124, 665-672, 1997) . Previous genetic and morphological characterizations of tso1 mutants and recent molecular isolation of the TSO1 gene indicate that TSO1 plays a regulatory role in cell division regulation, cytokinesis and inflorescence meristem organization. Potential postdoctoral projects will be the molecular and biochemical analyses of the TSO1 gene products and genetic screens for interacting genes and downstream targets. For further information, please email Dr. Zhongchi Liu at ZL17@umail.umd.edu or contact her at the Wisconsin Arabidopsis meeting (poster # 359) The position is for three years with an annual salary of US$ 30,861.00. University of Maryland at College Park is located in the midst of the Baltimore-Washington DC metropolitan area. It is 8 miles from the US capitol and about 25 miles from Baltimore. The proximity to Washington provides access and collaborative opportunities to many important federal research facilities such as NIH, USDA research center at Beltsville and Smithsonian Institute. The university is also about 45 minute drive to the Chesapeake Bay and within 2-3 hour drive to the mountains and good beaches on the Atlantic coast. To apply, please send a CV, a brief statement of research experience and research interests, and the names of three references (including address, phone, and email) to Dr. Zhongchi Liu by mail or email. Successful postdoctoral candidates should be highly motivated individuals with a Ph.D. degree and a proven record of productivity such as first-authored publications in internationally recognized journals. Experiences either in biochemistry or in plant molecular genetics are a plus. Zhongchi Liu, Ph.D. Assistant Professor Dept. of Cell Biology and Molecular Genetics 3236 H.J. Patterson Hall University of Maryland College Park, MD 20742 PH: (301) 405-1586 FAX: (301) 314-9082 email: ZL17@umail.umd.edu http://www.life.umd.edu/cbmg/faculty/liu.html --- From owner-arab-gen@hgmp.mrc.ac.uk Sat Jun 24 00:58:49 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 0515A17A6E; Sat, 24 Jun 2000 00:58:46 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id AF30617A7C; Sat, 24 Jun 2000 00:58:45 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: cryu@acesag.auburn.edu (Choong-min Ryu) Subject: re:fully expanded leaves Date: 23 Jun 2000 22:34:05 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <3953D929.9AB3400F@acesag.auburn.edu> Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk Dear TJ Generally, I measure leaf surface area as one of growth parameter based on Dario Leister's method (Plant Physiol. Biochem. 1999. 37 (9), 671-678). They demonstrate that a novel system, comprising of digital video and image analysis could determine plant size non-destructively by measuring plant leaf area of A. thaliana ecotype. col-0 and Landsberg erecta. It was a cool method but I don't know if you can apply to measure your curling leaf. Take care. Choong-Min Ryu Auburn University --- From owner-arab-gen@hgmp.mrc.ac.uk Wed Jun 28 20:00:34 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id E9AB917AD1; Wed, 28 Jun 2000 20:00:30 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id C37CE17B0C; Wed, 28 Jun 2000 20:00:29 +0100 (BST) To: arab-gen@net.bio.net Newsgroups: bionet.genome.arabidopsis From: zhe@sfsu.edu ("Z. He") Subject: Postdoc position available Date: 24 Jun 2000 01:44:35 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-arab-gen@hgmp.mrc.ac.uk Precedence: bulk A postdoctoral position is available to study the molecular mechanisms of interactions between extracellular and intracellular compartments. The project will involve the functional analysis of a family of cell wall-associated receptor kinases (WAKs) in Arabidopsis using a combination of cellular and molecular genetic approaches (Plant Journal 14:55-64 (1998); PMB 39:1189-1196 (1999)). Applicants are required to have a Ph.D. or equivalent doctorate level degree, and are expected to have a strong background in molecular biology. Review of applicant materials will begin immediately and will continue until the position is filled. Send applications including a curriculum vita, a brief statement of research interests, and three letters of reference to: Zheng-Hui He, Ph.D. Department of Biology San Francisco State University San Francisco, CA 94132 zhe@sfsu.edu Phone: (415)338-6193 (office) 405-0365 (lab) Fax: (415)338-2295 San Francisco State University is EOE/ADA employer. Women and minorities are strongly encouraged to apply. From owner-arab-gen@hgmp.mrc.ac.uk Wed Jun 28 20:01:09 2000 Return-Path: