From owner-software@net.bio.net Fri Jan 01 22:00:00 1993
Path: biosci!kristoff
From: kristoff@net.bio.net (David Kristofferson)
Newsgroups: bionet.software
Subject: Re: (none)
Message-ID: <Jan.1.19.24.45.1993.7142@net.bio.net>
Date: 2 Jan 93 03:24:45 GMT
References: <20@toano.win.net>
Distribution: bionet
Organization: BIOSCI International Newsgroups for Biology
Lines: 25

mj@toano.win.net (Mary Jo Warsinsky) writes:

>INDEX BIO-SOFT

The BIOSCI FAQ sheets describe how to use the archives.  We do not run
LISTSERV here.

New users of BIOSCI/bionet may want to read the "Frequently Asked
Questions" or "FAQ" sheets for BIOSCI.  There are two FAQ sheets for
the newsgroup system.  FAQ I describes the general purpose and uses of
the BIOSCI/bionet newsgroups.  FAQ II provides details on how to
participate in these forums.  Both of these FAQs are available for
anonymous FTP from net.bio.net [134.172.2.69] in
pub/BIOSCI/biosci1.FAQ and pub/BIOSCI/biosci2.FAQ.  They may also be
requested by sending e-mail to biosci@net.bio.net (use plain English
for your request).  The FAQs are also posted on the first of each
month to the newsgroup BIONEWS/bionet.announce immediately following
the posting of the BIOSCI information sheet.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@net.bio.net

From owner-software@net.bio.net Fri Jan 01 22:00:00 1993
Path: biosci!CSC.FI!Rob.Harper
From: Rob.Harper@CSC.FI (Rob Harper)
Newsgroups: bionet.software
Subject: Best of MSDOS (Part 2)
Message-ID: <199301021032.AA12553@csc.fi>
Date: 2 Jan 93 10:32:56 GMT
Sender: daemon@net.bio.net
Distribution: bionet
Lines: 826


>Xref: funic comp.archives.msdos.d:274 comp.binaries.ibm.pc.wanted:8872 comp.os.msdos.apps:7771 news.answers:4582
>Path: funic!fuug!mcsun!uknet!bnr.co.uk!pipex!uunet!gatech!destroyer!cs.ubc.ca!newsserver.sfu.ca!kko
>From: kko@fraser.sfu.ca (Samuel Ko)
>Newsgroups: comp.archives.msdos.d,comp.binaries.ibm.pc.wanted,comp.os.msdos.apps,news.answers
>Subject: The Most Useful MSDOS Programs at Simtel20 and Garbo (Part II)
>Summary: A list of recommended msdos programs at major ftp sites.
>Keywords: Simtel20, Garbo, sharewares, freewares, PD programs, ftp
>Message-ID: <1993Jan1.231803.2642@sfu.ca>
>Date: 1 Jan 93 23:18:03 GMT
>Sender: news@sfu.ca
>Followup-To: comp.archives.msdos.d
>Organization: Simon Fraser University, Burnaby, B.C., Canada
>Lines: 810
>Approved: news-answers-request@MIT.Edu

Archive-name: msdos-archives/part2
Last-modified: Jan 1, 1993
Version: 2.0

     The Most Useful MSDOS Programs at Simtel20 and Garbo (Part II)
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
       ****   The Ultimate Guide to MSDOS Archive Files   ****

Version: 2.0  Jan 1, 1993

Compiled by Samuel Ko (kko@sfu.ca)

Contents:

Part II
-------
* Section C  Utilities
   Area 5:  Screen Utilities
   Area 6:  System Utilites
   Area 7:  Other Utilities
* Section D:  Anti-Virus Programs
* Section E:  Editors
* Section F:  Communication Programs
* Section G:  Miscellaneous Files
----
* Appendix 1:  Mirror Sites
* Appendix 2:  Obtaining files by e-mail
* Appendix 3:  Getting upload announcements by e-mail
* Appendix 4:  How to do anonymous ftp?

---------------------------------------------------------------------------
   Area 5:  Screen Utilities
---------------------------------------------------------------------------
Program: Explosiv Screen Saver (for DOS and Windows)
Version: 2.10
Upload Date: 92/10/08
File: explo210.zip
Size: 272,258 bytes
Location: oak: /pub/msdos/screen
          garbo: /pc/screen
Status: Shareware
Comment: Marvelous ... lots of displays ...
Alternatives: Explod Fireworks (explo203.zip in /pc/graphics) (garbo only)
              Billboard (bilbd110.zip) (oak only) - will display message
---------------------------------------------------------------------------
Program: Buffit - tsr screen scrollback utility
Version: 3.0
Upload Date: 91/05/21
File: buffit30.zip
Size: 10,640 bytes
Location: oak: /pub/msdos/screen
          garbo: /pc/screen
Status: Shareware
Comment: Good ...
---------------------------------------------------------------------------
Program: Snipper
Version: 2.6
Upload Date: 92/10/28
File: snippr26.zip
Size: 20,881 bytes
Location: oak: /pub/msdos/screen
          garbo: /pc/screen
Status: Freeware
Comment: Marvelous ... Can capture a text screen to the printer, a file,
         or internal buffer ...
---------------------------------------------------------------------------
   Area 6:  System Utilities
---------------------------------------------------------------------------
Program: InfoPlus - screens of system info
Version: 1.56
Upload Date: 92/12/30
File: ifp1p156.zip
Size: 127,580 bytes
Location: oak: /pub/msdos/info
          garbo: /pc/sysinfo
Status: Freeware
Comment: Good ...
Alternatives: Comptest (ctest257.zip)
              ASQ (asq130.zip)
              PC Mag Lab Benchmarks (pcben60{a,b,c}.zip; bench60.zip (garbo))
              Microsoft Diagnostics (msd.zip) ... this one is available
              from ftp-os2.nmsu.edu (in /pub/os2/2.0/dos/sysutils) ...
---------------------------------------------------------------------------
Program: Secure System Login
Version: 1.0
Upload Date: 92/08/29
File: login.zip
Size: 71,258 bytes
Location: oak: /pub/msdos/security
          garbo: /pub/sysutil
Status: Shareware, Free for personal use
Comment: Good ... unix-like ... login: ?  Password: ? ...
Alternative: CCP (ccp11.zip) - a more secure system control program
---------------------------------------------------------------------------
Program: HyperDisk
Version: 4.51
Upload Date: 92/12/07
File: spkt451s.zip
Size: 291,958 bytes
Location: oak: /pub/msdos/dskutl
          garbo: /pc/sysutil (not the latest)
Status: Shareware
Comment: Disk caching utilities ... better than the RAM Disk ... And
         other Speedkit's utilities (HyperKey and HyperScreen) included ...
         BTW ... Cache Test (ct43c.zip) is good for evaluating
         your disk's cache performance ...
---------------------------------------------------------------------------
Program: TSR Utilities
Version: 3.4
Upload Date: 92/03/12
File: tsrcom34.zip
Size: 75,275 bytes
Location: oak: /pub/msdos/sysutl
          garbo: /pc/memutil
Status: Freeware
Comment: Good ... Can remove TSRs from memory without rebooting ...
---------------------------------------------------------------------------
Program: File Completion
Version: 1.5
Upload Date: 92/08/07
File: filec15.zip
Size: 19,564 bytes
Directory: oak: /pub/msdos/sysutl
           garbo: /pc/keyboard
Status: Freeware
Comment: A good TSR program to add filename completion ...
---------------------------------------------------------------------------
Program: Shell Room
Version: 2.0a
Upload Date: 92/08/19
File: shroom20.zip
Size: 920819
Directory: oak: /pub/msdos/sysutl
           garbo: /pc/sysutil
Status: Shareware
Comment: Can swap programs from memory to disk ...
---------------------------------------------------------------------------
   Area 7:  Other Utilities
---------------------------------------------------------------------------
Program: PC ReadMail
Version: 4.01
Upload Date: 91/07/07
File: rmail401.zip
Size: 68,981 bytes
Location: oak: /pub/msdos/txtutl
          garbo: /pc/mail
Status: Freeware
Comment: A very good program for reading Unix mails, netnews articles and
         Timo's FAQs OFFLINE ...
---------------------------------------------------------------------------
Program: Cal (unix-like)
Version: 2.4
Upload Date: 92/07/11
File: cal24.zip
Size: 20,730 bytes
Location: oak: /pub/msdos/deskaccess
          garbo: /pc/unix/calendar or /pc/unix
Status: Freeware
Comment: This program can have the current date highlighted on the monthly
         calendar and a list of important dates displayed ...
---------------------------------------------------------------------------
Program: Ample Notice
Version: 3.0
Upload Date: 92/10/07
File: an300.zip
Size: 192,263 bytes
Location: oak: /pub/msdos/deskaccess
          garbo: /pc/calendar
Status: Shareware
Comment: A good appointments calendar / alarm clock package ...
---------------------------------------------------------------------------
Program: Ultimate Calculator
Version: 1.2
Upload Date: 92/10/27
File: ucalc12.zip
Size: 53,551 bytes
Location: oak: /pub/msdos/calculator
          garbo: /pc/math
Status: Shareware
Comment: A high precision scientific calculator which you can operate
         at the command prompt ...
Alternative: MEC (mathfc23.zip)
---------------------------------------------------------------------------
Program: Thumb Print Software's Electric Typewriter
Version: 1.0
Upload Date: 91/02/09
File: tp-et.zip
Size: 115,774 bytes
Location: oak: /pub/msdos/education
          garbo: /pc/editor
Status: Shareware
Comment: This program "emulates" an electronic type-writer ...
---------------------------------------------------------------------------
Program: TypeFast
Version: -
Upload Date: 90/09/12
File: typefast.zip
Size: 91,532 bytes
Location: oak: /pub/msdos/education
          garbo: /pc/educgames
Status: Public Domain
Comment: A game that can improve your typing speed ...
--------------------------------------------------------------------------
Program: The Animated Series
Version: 1.0
Upload Date: 92/11
Files: aalpha.zip, amath1.zip. amem10.zip, animshap.zip, awcmp{1,2}.zip
Size: various
Location: oak: /pub/msdos/education
          garbo: /pc/educgames
Status: Shareware
Comment: Animated programs to enhance learning for preschoolers ...
         These are just several examples of educational programs available
         from Simtel20 and Garbo ... There are many others in the above
         directories (and /../teaching at oak) ... My latest favorite is
         MathShop (mathsp11.zip) :) ...
---------------------------------------------------------------------------
Program: SkyGlobe
Version: 3.52
Upload Date: 92/10/16
File: skygl352.zip
Size: 297,105 bytes
Location: oak: /pub/msdos/astronomy
          garbo: /pc/astronomy
Status: Shareware
Comment: An easily accessible planetarium displaying stars ...
Alternative: COSMOS (cosmos16.zip)
---------------------------------------------------------------------------
Program: ACCU Music System
Version: 3.1
Upload Date: 92/12/10
File: ac31musc.zip
Location: oak: /pub/msdos/music
          garbo: not available
Status: Shareware
Comment: Create, print, transpose and play music ...
---------------------------------------------------------------------------
Section D:  Anti-Virus Programs
---------------------------------------------------------------------------
Note: New versions for these programs are released pretty frequently ...
      (usually once a month) ...
---------------------------------------------------------------------------
Programs: McAfee anti-virus utilities
Version: 99
Upload Date: 92/12/02
Files: scanv99.zip(scanner), clean99.zip(removal), vshld99.zip(prevention)
Size: scan(158,423 bytes), clean(172,556 bytes), vshield(121,575bytes)
Location: oak: /pub/msdos/trojan-pro
          garbo: /pc/virus
Status: Sharewares
Comment: Absolutely and positively the BEST ...
         BTW ... McAfee's anti-virus utilities can also be obtained from
         /pub/antivirus at the ftp site mcafee.com ...
---------------------------------------------------------------------------
Program: F-Prot anti-virus program
Version: 2.06a
Upload Date: 92/11/06
File: fp-206a.zip
Size: 312,066 bytes
Location: oak: /pub/msdos/trojan-pro
          garbo: /pc/virus
Status: Shareware, Free for private use
Comment: a MUST ...
Alternative: Thunderbyte A-V (tbav502.zip + vsig9214.zip)
---------------------------------------------------------------------------
Program: VIRx
Version: 2.6
Upload Date: 92/12/07
File: virx26.zip
Size: 103,915 bytes
Location: oak: /pub/msdos/trojan-pro
          garbo: /pc/virus
Status: Freeware
Comment: A fine virus scanner ...
---------------------------------------------------------------------------
Program: HyperText VSUM
Version: 9212
Upload Date: 92/12/30
File: vsumx212.zip
Size: 746,332 bytes
Location: oak: /pub/msdos/trojan-pro
          garbo: /pc/virus (not the latest)
Status: Shareware
Comment: Organized in hypertext form, this program tells you everything you
         ever want to know about viruses ... Also availabe at mcafee.com ...
         (in /pub/vsum) ...
---------------------------------------------------------------------------
Section E:  Editors
---------------------------------------------------------------------------
Program: QEdit
Version: 2.15
Upload Date: 91/09/09
File: qedit215.zip
Size: 134,778 bytes
Location: oak: /pub/msdos/qedit
          garbo: /pc/editor
Status: Shareware
Comment: This one is very very good ... I'm using it now :) ...
Alternatives: can be found in oak: /pub/msdos/editor
              Vision-Edit (ved331.zip)  - can edit files up to 4 megabytes
              Video Display Editor (vde164.zip) - an efficient editor/quasi-
              word-processor
              Boxer (box400b.zip) - a good, fully functional editor
              PDE (pde
From owner-software@net.bio.net Fri Jan 01 22:00:00 1993
Path: biosci!CSC.FI!Rob.Harper
From: Rob.Harper@CSC.FI (Rob Harper)
Newsgroups: bionet.software
Subject: Best of MSDOS (Part1)
Message-ID: <199301021031.AA12529@csc.fi>
Date: 2 Jan 93 10:31:26 GMT
Sender: daemon@net.bio.net
Distribution: bionet
Lines: 755


>Xref: funic comp.archives.msdos.d:271 comp.binaries.ibm.pc.wanted:8870 comp.os.msdos.apps:7769 news.answers:4580
>Newsgroups: comp.archives.msdos.d,comp.binaries.ibm.pc.wanted,comp.os.msdos.apps,news.answers
>Path: funic!fuug!mcsun!uunet!zaphod.mps.ohio-state.edu!swrinde!gatech!destroyer!cs.ubc.ca!newsserver.sfu.ca!kko
>From: kko@fraser.sfu.ca (Samuel Ko)
>Subject: The Most Useful MSDOS Programs at Simtel20 and Garbo (Part I)
>Message-ID: <1993Jan1.231418.2527@sfu.ca>
>Followup-To: comp.archives.msdos.d
>Summary: A list of recommended msdos programs at major ftp sites. 
>Keywords: Simtel20, Garbo, sharewares, freewares, PD programs, ftp
>Sender: news@sfu.ca
>Organization: Simon Fraser University, Burnaby, B.C., Canada
>Date: Fri, 1 Jan 1993 23:14:18 GMT
>Approved: news-answers-request@MIT.Edu
>Lines: 739

Archive-name: msdos-archives/part1
Last-modified: Jan 1, 1993
Version: 2.0

     The Most Useful MSDOS Programs at Simtel20 and Garbo (Part I)
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
       ****   The Ultimate Guide to MSDOS Archive Files   ****

Version: 2.0  Jan 1, 1993  (so, Happy New Year!!!)

Compiled by Samuel Ko (kko@sfu.ca)

This is a good selection of the "best" sharewares and freewares that are
available from Simtel20 (wsmr-simtel20.army.mil) - the largest depository
of MSDOS files - and Garbo (garbo.uwasa.fi) - a good alternative to Simtel20.
This list contains many commonly-and-frequently-wanted sharewares/freewares/
public domain programs. So before you post any requests for ftp'able MSDOS
files in comp.archives.msdos.d, comp.binaries.ibm.pc.wanted or other msdos/pc
newsgroups, please try to read the list first. This will help reduce the
traffic in those groups a bit. Besides you might find some other interesting
stuff here.

As it is more convenient to ftp files from Simtel20's mirror sites than
Simtel20 itself, so I use the directories of oak.oakland.edu (the most
up-to-date mirror site) instead. If you want to use the parent site (Simtel20)
or other mirror sites (e.g. wuarchive.wustl.edu), please change the path name
accordingly. Here is how to do it:  (`whatever' is the name of subdirectory
to be substituted)

oak: /pub/msdos/whatever = Simtel20: PD1:<MSDOS.WHATEVER>
                         = wuarchive: /mirrors/msdos/whatever

Please note that garbo is mirrored in N. America at wuarchive. Therefore
garbo: /pc/whatever = wuarchive: /mirrors/garbo.uwasa.fi/whatever

For info on other Simtel20 and Garbo mirror sites, please see Appendix 1.

If you do not have ftp access, please read Appendix 2 for instructions
on how to obtain most of the files listed below by e-mail.

Thanks to all who have helped in improving the list. However, to save
some bandwidth, I do not want to include a long list (over 150 names) of
acknowledgment. I do sincerely thank each and every one of you who has
contributed to this compilation in one way or the other.

Any further comments and suggestions will be very much appreciated. I am
particularly interested in any programs that you think are better than those
listed below.

I will usually update this list once a month. So, the next issue will be
out in early February.

General format of an entry:
---------------------------------------------------------------------------
Program:  Name and/or Description
Version:  The latest release
Upload Date:  The date the program was uploaded to Simtel or Oak
File:  The name of the file that corresponds to the program
Size:  The size of the archive file
Location:  The directory from which the file can be found
Status:  Shareware / Freeware Info
         Shareware - copyrighted (or patented) programs distributed and sold
         on a "try before you buy" basis.
         Freeware - copyrighted programs which do not require compensation
         for use in most cases.
         Public Domain - free from copyrights; open to use by anyone
         without compensation.
Comment:  My comment and supplementary info
Alternative:  Other similar program
---------------------------------------------------------------------------------
Contents:

Part I
------
* Section A:  Application Programs
* Section B:  Compression / Decompression Programs
* Section C:  Utilities
   Area 1:  4DOS
   Area 2:  Disk Utilites
   Area 3:  Directory Utilities
   Area 4:  File / Text Utilities

[ Part II
  -------
   Area 5:  Screen Utilites
   Area 6:  System Utilites
   Area 7:  Other Utilities
* Section D:  Anti-Virus Programs
* Section E:  Editors
* Section F:  Communication Programs
* Section G:  Miscellaneous Files
--
* Appendix 1:  Mirror Sites
* Appendix 2:  Obtaining files by e-mail
* Appendix 3:  Getting upload announcements by e-mail
* Appendix 4:  How to do anonymous ftp?  ]

---------------------------------------------------------------------------
Section A:  Application Programs
---------------------------------------------------------------------------
Program: PC-Write Advanced Level Word-Processor
Version: 4.0
Upload Date: 92/07/24
Files: pcwal401.zip (Program), pcwal402.zip (Help/Spell)
       pcwal1403.zip (Conversion), pcwal404.zip (Printer)
Size: 946,024 bytes (total)
Location: oak: /pub/msdos/editor
          garbo: /pc/txtutil
Status: Shareware
Comment: PC-Write is undoubtedly one of the most sophisticated word-processor
         There is a simpler version - pcws201*.zip (oak only) ...
---------------------------------------------------------------------------
Program: AsEasyAs Spreadsheet
Version: 5.01c
Upload Date: 92/07/08
File: aea501c.zip
Size: 338,568 bytes
Location: oak: /pub/msdos/spreadsheet
          garbo: /pc/spreadsheet
Status: Shareware
Comment: A powerful Lotus 123 clone ...
         There is a "lighter" version - alite1f.zip (oak only) ...
---------------------------------------------------------------------------
Program: Buttonware's PC-File Database
Version: 6.5
Upload Date: 92/08/11
Files: pcf65{a,b,c}.zip
Size: 895,990 bytes  (total)
Location: oak: /pub/msdos/dbase
          garbo: /pc/database
Status: Shareware
Comment: This database program is fine as long as you've got disk-space
         for it ...
Alternative: RExL (rexl211{a,b,c}.zip in /pub/msdos/database) (oak only)
             File expresss (fe51-{a,b,c}.zip) (ditto)
---------------------------------------------------------------------------
Program: EnVision Publisher
Version: 1.0
Upload Date: 92/12/02
Files: evp10{a,b}.zip
Size: 669,413 bytes (total)
Location: oak: /pub/msdos/deskpub
          garbo: not available
Status: Shareware
Comment: Great! ... A WYSIWYG full-feature desktop publishing program ...
---------------------------------------------------------------------------
Program: Draft Choice CAD
Version: 2.0
Upload Date: 92/07/15
File: dc20.zip
Size: 314,791 bytes
Location: oak: /pub/msdos/cad
          garbo: /pc/cad
Status: Shareware
Comment: A good computer-assisted-design program ... Get dcfonts.zip ...
BTW ... Data flow diagrams ...
        - Analyst's Toolkit (atk15ibm.zip in /pub/msdos/flowchart) - good
        - EasyCase (ezcase17.zip in /pub/msdos/case) - limited CASE
        Flow-Charts ...
        - FLODRAW (fdrw225{a,b}.zip - /pub/msdos/flowchart) (CGA only?)
---------------------------------------------------------------------------
Program: X(Plore) Mathematical Toolchest
Version: 4
Upload Date: 92/06/08
File: cc4-9206.zip
Size: 417,716 bytes
Location: oak: /pub/msdos/calculator
          garbo: /pc/math
Status: Shareware
Comment: A sophisticated calculus calculator ... doing numeric math ...
Alternatives: Symbolic Calculator (sm211a.zip) - doing symbolic math
              Mercury (mrcry209.zip) - solving equations (in /.../math for oak)
---------------------------------------------------------------------------
Section B:  Compression / Decompression Programs
---------------------------------------------------------------------------
Synopsis - DOS and UNIX
~~~~~~~~
                  DOS                                UNIX
                  ---                                ----
*.zip          pkz110eu.exe  (pkz193a.exe)        zip10ex.tar-z (or .tar.Z)
               zip10exx.zip  (zip19p1x.zip)       zip19p1.tar-z (or .tar.Z)
               unzip50.exe                        unzip50.tar-z (or .tar.Z)

*.arj          arj230ng.exe (arj239a.exe)         --
               unarj230.zip                       unarj230.tar-z (or *.tar.Z)

*.lzh          lha213.exe                         lha-101u.tar-z (oak)
                                                  lha-1.00.tar.Z (garbo)

*.arc          pk361.exe                          arc521e.tar-z (oak)
               arc602.exe                         arc.tar.Z (garbo)

*.zoo          zoo210.exe                         zoo210.tar-z (or .tar.Z)
               booz20.zip                         booz20.tar-z (or .Z)

*.hpk          hpack78.zip                        hpack78src.tar.Z (garbo only)

*.sqz          sqz1082e.exe                
From owner-software@net.bio.net Fri Jan 01 22:00:00 1993
Path: biosci!agate!ames!purdue!yuma!neptune.rrb.colostate.edu!elpeters
From: elpeters@lamar.colostate.edu (Eric L. Peters)
Newsgroups: bionet.software
Subject: The Vertebrate World
Message-ID: <Jan02.215130.20331@yuma.ACNS.ColoState.EDU>
Date: 2 Jan 93 21:51:30 GMT
Sender: news@yuma.ACNS.ColoState.EDU (News Account)
Organization: Colorado State University
Lines: 100
X-Useragent: Nuntius v1.1.1d16
X-Xxdate: Sat, 2 Jan 93 14:54:32 GMT
X-Xxmessage-Id: <A76B5D287B017D06@neptune.rrb.colostate.edu>
Nntp-Posting-Host: neptune.rrb.colostate.edu


              ANNOUNCING THE VERTEBRATE WORLD PROJECT

The Vertebrate World is a HyperCard stack that is designed to be a
database shell for the vertebrate animals.  The database as planned
will eventually provide taxonomic, ecological, behavioral and
distribution information for thousands of species of vertebrates.
Digitized pictures of each species will be available, as well as
phylogenetic trees, sounds, QuickTime movies, and range maps for many
species.  This will (obviously) require storage and distribution on
large capacity medium such as CD-ROM discs.  Because of the size of
the database, and the relative scarcity of CD-ROM drives, my plan is
to allow this database to be customizable by individual instructors by
creating a 'master control' stack.  The instructor can use this front
end to choose particular species for inclusion into self-running
subsets of the entire database.  These can then be run from a hard
disk drive.  For example, an instructor might select individual
species, or make inclusion criteria for species that correspond to
specified search terms, such as 'Birds of North Carolina' or
'Endangered Vertebrates of North America'.  The master control would
then automatically generate a new HyperCard stack containing those
species' entries, and this user-generated stack could then be
distributed to students or copied onto hard drives in a microcomputer
lab.  Various template stacks that automatically include only
information appropriate for use at particular education levels will be
provided, and these will be modifiable by the instructor.

                  THE VERTEBRATE WORLD PROJECT

As there are so many species of vertebrates, I would like to 'job out'
different segments of the project to different people. The Vertebrate
World Project will have several areas of potential contributions:
writing and editing the species accounts, constructing phylogenetic
data files, and locating and digitizing pictures, videos and sounds.
For example, one person might be in charge of writing the accounts of
the pinnepeds, another might collect and/or scan pictures of
Australian snakes, etc.

In return for these efforts, each person contributing a certain amount
of effort would obtain a copy of the finished product of their choice
(i.e., one disk from the series), as well as acknowledgement in the
documentation. Persons contributing a large amount of digitized or
(especially) original written material would be listed as co-authors
of the overall project.

I believe this is the best approach for us to take because it places
the responsibility for the stacks' contents in the hands of experts in
each taxon, and it prevents duplication of effort.  I also think it
would be most worthwhile to be able to offer a complete set (perhaps
on several disks according to Class, with the possibilty of doing it
by subscription as new species are added).  I think that this series
would be an excellent marketing tool for a publisher to convince
instructors to buy textbooks on ecology or vertebrate biol
From owner-software@net.bio.net Fri Jan 01 22:00:00 1993
Path: biosci!CSC.FI!Rob.Harper
From: Rob.Harper@CSC.FI (Rob Harper)
Newsgroups: bionet.software
Subject: Best of MSDOS (Changes and updates)
Message-ID: <199301021038.AA12616@csc.fi>
Date: 2 Jan 93 10:38:22 GMT
Sender: daemon@net.bio.net
Distribution: bionet
Lines: 510


>Xref: funic comp.archives.msdos.d:273 comp.binaries.ibm.pc.wanted:8871 comp.os.msdos.apps:7770 news.answers:4581
>Newsgroups: comp.archives.msdos.d,comp.binaries.ibm.pc.wanted,comp.os.msdos.apps,news.answers
>Path: funic!fuug!mcsun!uunet!cs.utexas.edu!swrinde!gatech!destroyer!cs.ubc.ca!newsserver.sfu.ca!kko
>From: kko@fraser.sfu.ca (Samuel Ko)
>Subject: The Most Useful MSDOS Programs at Simtel20 and Garbo (Changes)
>Message-ID: <1993Jan1.232159.2724@sfu.ca>
>Followup-To: comp.archives.msdos.d
>Summary: A list of major changes since version 1.9. 
>Keywords: changes, additions, updates
>Sender: news@sfu.ca
>Organization: Simon Fraser University, Burnaby, B.C., Canada
>Date: Fri, 1 Jan 1993 23:21:59 GMT
>Approved: news-answers-request@MIT.Edu
>Lines: 494

Archive-name: msdos-archives/diff
Last-modified: Jan 1, 1993

    The Most Useful MSDOS Programs at Simtel20 and Garbo (Changes)
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
        ****  The Ultimate Guide to MSDOS Archive Files  ****

Version: 2.0  Jan 1, 1993

Compiled by Samuel Ko (kko@sfu.ca)

As this most-useful-program list is quite long, I'm posting this article
listing major changes (additions and updates) for those of you who have
read the previous issue (version 1.9  Oct 24, 1992).

The information in different msdos-archives postings should be more or less
accurate as of Dec 31, 1992.  As the world of MSDOS archives is rapidly
changing, there will be lots of changes before the next version is released.
To keep yourself informed of the latest and greatest of MSDOS archive files,
do read the upload announcements on comp.archives.msdos.announce newsgroup
(or subscribe to the MSDOSANN mailing list if you do not have netnews access).

----
     The Most Useful MSDOS Programs at Simtel20 and Garbo (Part I)
  ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
[ a modified title ]
[ the compilation is now in 2 parts ]

General format of an entry:
---------------------------------------------------------------------------
...
Status:  Shareware / Freeware Info
         Shareware - copyrighted (or patented) programs distributed and sold
         on a "try before you buy" basis.
         Freeware - copyrighted programs which do not require compensation
         for use in most cases.
         Public Domain - free from copyrights; open to use by anyone
         without compensation.
--------------------------------------------------------------------------
Section A:  Application Programs
--------------------------------------------------------------------------
Program: Buttonware's PC-File Database
...
Alternative: ...
             File expresss (fe51-{a,b,c}.zip) (ditto)    [ new ]
---------------------------------------------------------------------------
Program: EnVision Publisher    [ new ]
Version: 1.0
Upload Date: 92/12/02
Files: evp10{a,b}.zip
Size: 669,413 bytes (total)
Location: oak: /pub/msdos/deskpub
          garbo: not available
Status: Shareware
Comment: Great! ... A WYSIWYG full-feature desktop publishing program ...
---------------------------------------------------------------------------
Program: Draft Choice CAD
...
BTW ... Data flow diagrams ...
        - Analyst's Toolkit (atk15ibm.zip in /pub/msdos/flowchart) - good
        - EasyCase (ezcase17.zip in /pub/msdos/case) - limited CASE
        Flow-Charts ...
        - FLODRAW (fdrw225{a,b}.zip - /pub/msdos/flowchart) (CGA only?)
[ new ]
---------------------------------------------------------------------------
Program: X(Plore) Mathematical Toolchest
...
Alternatives: ...
              Mercury (mrcry209.zip) - solving equations (in /.../math for oak)
[ new ]
---------------------------------------------------------------------------
Section B:  Compression / Decompression Programs
---------------------------------------------------------------------------
Synopsis - DOS and UNIX
~~~~~~~~
*.hpk          hpack78.zip                        hpack78src.tar.Z (garbo only)

*.F            --                                 freeze *

*.z            --                                 pack (on your system)
               --                                 gzip-0.5.tar.Z **

*.exe/         pklte115.zip                       --
*.com          lzexe91e.zip
               diet144.zip

* freeze is a new Unix compression program ... Get it from
  /usenet/comp.sources.misc/volume25/freeze at wuarchive ...

** gzip (GNU zip) is designed to replace Unix's compress (?) ... Get gzip
   from prep.ai.mit.edu (in /pub/gnu) ...
[ new ]
---------------------------------------------------------------------------
Program: Hpack multisystem archiver    [ new ]
Version: 0.78
Upload Date: (to garbo) 92/11/20
File: hpack078.zip
Size: 114,243
Location: oak: /pub/msdos/arc-lbr (not the latest)
          garbo: /pc/arcers
Status: Freeware
Comment: yet another fine archiver ... but slow compression :( ...
---------------------------------------------------------------------------
Program: Buerg's archive files viewer
Version: 1.41    [ update ]
Upload Date: 92/11/08
File: fv141.zip
--------------------------------------------------------------------------
Program: ArcMaster
Version: 8.3   [ update ]
Upload Date: 92/11/08
File: am83.zip
...
Alternative: Shez (shez83.zip)    [ update ]
---------------------------------------------------------------------------
Section C:  Utilities
---------------------------------------------------------------------------
   Area 1:  4DOS
---------------------------------------------------------------------------
Program: 4DOS command.com replacement
Version: 4.01 revision d    [ update ]
Upload Date: 92/10/31
File: 4dos401p.zip
---------------------------------------------------------------------------
   Area 2:  Disk Utilites
---------------------------------------------------------------------------
Program: OnePass - single-pass floppy-disk copier
...
Alternatives: ... , WWScopyd (copyd11.zip)
BTW ... A good program to break a large file up into pieces and copies it to
floppies is  slice  (oak: /pub/msdos/pcmag/slice13.arc) ...    [ new ]
---------------------------------------------------------------------------
Program: MegaBack disk system backup
Version: 2.1    [ update ]
Upload Date: 92/12/07
File: mback210.zip
---------------------------------------------------------------------------
Program: HDTEST    [ new ]
Version: 5.35a
Upload Date: 92/10/26
File: hdts535a.zip
Size: 139,775
Location: oak: /pub/msdos/dskutl
          garbo: /pc/diskutil (not the latest)
Status: Shareware
Comment: A good program for testing and maintaining disks ...
---------------------------------------------------------------------------
   Area 3:  Directory Utilities
---------------------------------------------------------------------------
Program: Vernon Buerg's file viewer and disk utility
Version: 7.7a    [ update ]
Upload Date: 92/10/26
File: list77a.zip
---------------------------------------------------------------------------
   Area 4:  File / Text Utilities
---------------------------------------------------------------------------
Program: Derr's FileFinder
Version: 4.1    [ update ]
Upload Date: 92/11/14
File: fff41.zip
---------------------------------------------------------------------------
Program: Pretty Good Privacy    [ new ]
Version: 2.1
Upload Date: (to garbo) 92/12/07
File: pgp21.zip
Size: 187,758 bytes
Location: oak: not available
          garbo: /pc/fileutil
Status: Freeware
Comment: This is a very popular but controversial (public key) encrytpion
         program ...also available from ghost.dsi.unimi.it (in /pub/security)
Alternatives: Encrypt (encrypt.zip), Tangle (tangle.zip)
---------------------------------------------------------------------------
Program: Dosnx - Unix-like utilities
...
BTW ... Some other "common" Unix Tools for Dos:
                   ^^^^^^^^^^^^^^^^^^^^
 ...
 ksh     -  oak: /pub/msdos/sysutl/ms_sh21b.zip (386+ preferred)   [ update ]
 ...
 rcs     -  oak: /pub/msdos/pgmutl/rcs56dos.zip    [ update ]
---------------------------------------------------------------------------
Program: CompuShow *.gif files viewer
...
Alternatives: ...
              GIFV (gifv37.zip)    [ new ]

BTW ... a bit on jpeg files ...
viewers:  ...
          ColorView (dcview20.zip) - oak: /pub/msdos/graphics    [ new ]
          DVPEG (dvpeg21.zip) - sunee.uwaterloo.ca: /pub/jpeg/viewers [ update ]
---------------------------------------------------------------------------


     The Most Useful MSDOS Programs at Simtel20 and Garbo (Part II)
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
       ****   The Ultimate Guide to MSDOS Archive Files  ****

---------------------------------------------------------------------------
   Area 5:  Screen Utilities
---------------------------------------------------------------------------
Program: Snipper    [ update ]
Version: 2.6
Upload Date: 92/10/28
File: snippr26.zip
---------------------------------------------------------------------------
   Area 6:  System Utilities
---------------------------------------------------------------------------
Program: InfoPlus - screens of system info
Version: 1.56    [ update ]
Upload Date: 92/12/30
File: ifp1p156.zip
...
Alternatives: ...
              ASQ (asq130.zip)
              PC Mag Lab Benchmarks (pcben60{a,b,c}.zip; bench60.zip (garbo))
[ new ]
---------------------------------------------------------------------------
Program: Secure System Login
...
Alternative: CCP (ccp11.zip) - a more secure system control program  [ new ]
---------------------------------------------------------------------------
Program: HyperDisk
Version: 4.51    [ update ]
Upload Date: 92/12/07
File: spkt451s.zip
---------------------------------------------------------------------------
   Area 7:  Other Utilities
---------------------------------------------------------------------------
Program: Ultimate Calc
From owner-software@net.bio.net Fri Jan 01 22:00:00 1993
Path: biosci!agate!stanford.edu!enterpoop.mit.edu!eru.mt.luth.se!lunic!sunic!dkuug!uts!engeje
From: engeje@uts.uni-c.dk (Jacob Engelbrecht)
Newsgroups: bionet.software
Subject: Re: intron/exon borders
Message-ID: <1993Jan2.212630.683@uts.uni-c.dk>
Date: 2 Jan 93 21:26:30 GMT
References: <30DEC199214434286@aardvark.ucs.uoknor.edu>
Organization: UNI-C, Danish Computing Centre for Research and Education
Lines: 257

In <30DEC199214434286@aardvark.ucs.uoknor.edu> bfrank@aardvark.ucs.uoknor.edu (FRANK,BART) writes:

>Can anyone recommend a good program to screen human genomic seqeunces
>and predict positions of intros/exon borders?

>Thanks, 
>Bart Frank
>Internet: BFRANK@AARDVARK.UCS.UOKNOR.EDU

I include information of a mail server service for prediction of
splice sites as described in our Journal of Molecular Biology article.


********       Announcement of the NetGene Mail-server:        *********

DESCRIPTION:

   The  NetGene  mail  server  is a  service  producing  neural  network
   predictions  of splice  sites in  vertebrate  genes as described  in:
   Brunak, S.,  Engelbrecht,  J., and Knudsen, S.  (1991)  Prediction of
   Human mRNA Donor and Acceptor  Sites from the DNA  Sequence.  Journal
   of Molecular Biology, 220, 49-65.


ABSTRACT OF JMB ARTICLE:

   Artificial  neural  networks have been applied to the  prediction  of
   splice site location in human  pre-mRNA.  A joint  prediction  scheme
   where  prediction  of transition  regions  between  introns and exons
   regulates  a cutoff  level for  splice  site  assignment  was able to
   predict splice site locations with confidence  levels far better than
   previously  reported in the  literature.  The  problem of  predicting
   donor and  acceptor  sites in human genes is hampered by the presence
   of  numerous   amounts  of  false   positives  -  in  the  paper  the
   distribution  of these false splice sites is examined and linked to a
   possible  scenario  for the  splicing  mechanism  in vivo.  When  the
   presented  method detects 95% of the true donor and acceptor sites it
   makes less than 0.1% false donor site  assignments and less than 0.4%
   false acceptor site assignments.  For the large data set used in this
   study this means that on the  average  there are one and a half false
   donor sites per true donor site and six false acceptor sites per true
   acceptor site.  With the joint assignment method more than a fifth of
   the true donor sites and around one fourth of the true acceptor sites
   could  be  detected  without  accompaniment  of  any  false  positive
   predictions.  Highly  confident  splice  sites  could not be isolated
   with a widely used weight  matrix  method or by separate  splice site
   networks.  A complementary  relation between the confidence levels of
   the  coding/non-coding  and the  separate  splice site  networks  was
   observed, with many weak splice sites having sharp transitions in the
   coding/non-coding  signal and many stronger  splice sites having more
   ill-defined transitions between coding and non-coding.


INSTRUCTIONS:

   In order to use the NetGene mail-server:

   1) Prepare a file with the sequence in a format  similar to the fasta
      format:  the first line must start  with the symbol  '>', the next
      word  on  that  line  is  used  as the  sequence  identifier.  The
      following lines should contain the actual sequence,  consisting of
      the symbols A, T, U, G, C and N.  U is converted to T, letters not
      mentioned  are converted to N.  All letters are converted to upper
      case.  Numbers,  blanks and other  nonletter  symbols are skipped.
      The lines  should not be longer than 80  characters.  The  minimum
      length  analyzed  is 451  nucleotides,  and the  maximum is 100000
      nucleotides  (your  mail  system  may have a lower  limit  for the
      maximum  size of a message).  Due to the  non-local  nature of the
      algorithm  sites  closer than 225  nucleotides  to the ends of the
      sequence will not be assigned.

   2) Mail the file to netgene@virus.fki.dth.dk.  The response time will
      depend on system  load.  If nothing else is running on the machine
      the  speed is about  1000  nucleotides/min.  It may  take  several
      hours  before you get the answer, so please do not  resubmit a job
      if you get no answer within a short while.


REFERENCING AND FURTHER INFORMATION

   Publication  of output from  NetGene must be  referenced  as follows:
   Brunak, S.,  Engelbrecht,  J., and Knudsen, S.  (1991)  Prediction of
   Human mRNA Donor and Acceptor  Sites from the DNA  Sequence.  Journal
   of Molecular Biology, 220, 49-65.


CONFIDENTIALITY
   Your  submitted  sequence  will be  deleted automatically immediately 
   after processing by NetGene.


PROBLEMS AND SUGGESTIONS:  

   Should be addressed to:

   Jacob Engelbrecht

   e-mail: engel@virus.fki.dth.dk

   Department of Physical Chemistry
   The Technical University of Denmark
   Building 206
   DK-2800 Lyngby
   Denmark

   phone: +45 4288 2222 ext. 2478 (operator)
   phone: +45 4593 1222 ext. 2478 (tone)
   fax:   +45 4593 4808

   
EXAMPLE:

   A file test.seq is prepared with an editor with the following contents:

   >HUMOPS
   GGATCCTGAGTACCTCTCCTCCCTGACCTCAGGCTTCCTCCTAGTGTCACCTTGGCCCCTCTTAGAAGC
   CAATTAGGCCCTCAGTTTCTGCAGCGGGGATTAATATGATTATGAACACCCCCAATCTCCCAGATGCTG
   . Here come more lines with sequence.
   .
   .
   
   This is sent to the NetGene mail-server, on a Unix system like this:
   mail netgene@virus.fki.dth.dk < test.seq
   
   In return an answer similar to this is produced:

From netgene@virus.fki.dth.dk Fri Mar 20 13:30 MET 1992
Received: by virus.fki.dth.dk
	(16.7/16.2) id AA05624; Fri, 20 Mar 92 13:30:41 +0100
Date: Fri, 20 Mar 92 13:30:41 +0100
From: virus mail server <netgene@virus.fki.dth.dk>
Return-Path: <netgene@virus.fki.dth.dk>
To: engel@virus.fki.dth.dk
Subject: HUMOPS: NetGene splice site prediction
Status: RO


------------------------------------------------------------------------
                               NetGene
               Neural Network Prediction of Splice Sites
                                                         
Reference: 
Brunak, S.,  Engelbrecht,  J., and  Knudsen, S.  (1991).  Prediction  of
Human mRNA donor and acceptor  sites from the DNA  sequence.  Journal of
Molecular Biology 220:49-65.
------------------------------------------------------------------------

Report ERRORS to Jacob Engelbrecht engel@virus.fki.dth.dk.

Potential splice sites are assigned by combining output from a local and
a global  network.  The  prediction is made with two cutoffs:  1) Highly
confident  sites (no or few false  positives, on average 50% of the true
sites  detected);  2) Nearly all true sites (more  false  positives - on
average of all positions 0.1% false positive  donor sites and 0.4% false
positive  acceptor  sites, at 95% detection of true sites).  The network
performance on sequences from distantly  related  organisms has not been
quantified.  Due to the  non-local  nature of the algorithm sites closer
than 225 nucleotides to the ends of the sequence cannot be assigned.



Column explanations, field identifiers: 

POSITION in your sequence (either first or last base in intron).
Joint CONFIDENCE level for the site (relative to the cutoff). 
EXON INTRON gives 20 bases of sequence around the predicted site.
LOCAL is the site confidence from the local network. 
GLOBAL is the site confidence from the global network. 

------------------------------------------------------------------------
The sequence: HUMOPS contains 6953 bases, and has the following composition:
A 1524 C 2022 G 1796 T 1611


1) HIGHLY CONFIDENT SITES:
==========================

ACCEPTOR SITES:
POSITION     CONFIDENCE        INTRON EXON         LOCAL   GLOBAL
    4094           0.27    TGTCCTGCAG^GCCGCTGCCC    0.63     0.66
    5167           0.20    TGCCTTCCAG^TTCCGGAACT    0.59     0.64
    3812           0.17    CTGTCCTCAG^GTACATCCCC    0.68     0.54
    3164           0.02    TCCTCCTCAG^TCTTGCTAGG    0.79     0.32
    2438           0.01    TGCCTTGCAG^GTGAAATTGC    0.78     0.33

DONOR SITES:
POSITION     CONFIDENCE          EXON INTRON       LOCAL   GLOBAL
    3979           0.38    CGTCAAGGAG^GTACGGGCCG    0.92     0.74
    2608           0.17    GCTGGTCCAG^GTAATGGCAC    0.85     0.54
    4335           0.06    GAACAAGCAG^GTGCCTACTG    0.83     0.41


2) NEARLY ALL TRUE SITES:
=========================

ACCEPTOR SITES:
POSITION     CONFIDENCE        INTRON EXON         LOCAL   GLOBAL
    4094           0.55    TGTCCTGCAG^GCCGCTGCCC    0.63     0.66
    3812         
From owner-software@net.bio.net Sat Jan 02 22:00:00 1993
Path: biosci!agate!spool.mu.edu!uunet!pipex!bnr.co.uk!uknet!comlab.ox.ac.uk!oxuniv!oxpath!jasper
From: jasper@vax.path.ox.ac.uk
Newsgroups: bionet.software
Subject: Protein Structure Prediction mailserver
Message-ID: <1993Jan2.173434.1@vax.path.ox.ac.uk>
Date: 2 Jan 93 16:34:34 GMT
Organization: Oxford University Molecular Biology Data Centre
Lines: 63
Nntp-Posting-Host: heatly
Nntp-Posting-User: jasper

Hi,

	I haven't *seen* this posted anywhere yet (apologies if it has been, 
I have that sneaking suspicion we are losing some articles through the feed) 
but the information is tucked away in Nature, so I think it needs advertising!

****  PHD - a new mail server for protein secondary structure prediction  ***

	First the picture:  for those of us who just run sequence through the
database and are then faced with one of Russ Doolittle's less attractive
options (Like "your protein does not match anything else yet seen in the
Universe" :) this is going to help you. But if you find that your protein is a
member of a family, and there is no homolgous 3D structure to work to, you are
kind of stuck with secondary structure predicitions that you *know* are only
going to be a little over half right, and you don't which half it's going to
be..... The better the prediction is going to be, the more useful it could be
to you. And if you have a measure for which parts are more likely to be
correct then that is going to help enormously. 

	So: and this is the end of a string of letters and papers that make
interesting reading in themselves; look at Nature, vol 360, page 540 (10th Dec
1992 issue) at the letter from Burkhard Rost and Chris Sander from EMBL
describing a new network server (PHD, Profile Neural Network Prediction) for
the prediction of secondary structures using information from multiple
sequence alignments. 

	To get started send HELP as the body of text to the server at 
PredictProtein@EMBL-Heidelberg.DE, and go from there. 

	You will be instructed on the correct format to send your sequence,
which will then be matched against the database, multiply aligned, the
prediction of secondary structure calculated and the whole thing sent back.
The return times are reasonably fast, but maybe that's because I'm testing it
in the vacation? Overall round trip time for a 200 amino acid domain is about
30 minutes. Of course this could get a lot slower if everyone tries it at 
once!

	One final point; the whole process will not work if your sequence has 
less than 30% idenitity to another member of the database. You may be in the 
position where your own favorite sequence is a member of a family, but at less 
than 30%. In which case, you should be able to learn something using one of 
the other family members; the results will not include 'your' protein, but if 
you already have alignments that are satisfactory this may be useful.

	I, for one, would be interested to know what sort of results people 
get from the system, and how well the outupt compares with a range of known or 
new 3D structures. Maybe as people get data they could feed this back to the 
group? Unless there is preference from EMBL as to where to send feedback?

	I hope I'm not stealing anyone's thunder by posting this, but it looks
too good to go unnoticed. 

	Well, many thanks to Burkhard and Chris, and New Years greetings to
everyone else! 

regards,    jasper

Jasper Rees
Oxford University Molecular Biology Data Centre (nights and weekends)
Sir William Dunn School of Pathology  (always)
Oxford University 

jasper@vax.path.ox.ac.uk

From owner-software@net.bio.net Sat Jan 02 22:00:00 1993
Path: biosci!CSC.FI!Rob.Harper
From: Rob.Harper@CSC.FI (Rob Harper)
Newsgroups: bionet.software
Subject: MSDOS uploads to SIMTEL20 DEC 1992
Message-ID: <199301031054.AA02546@csc.fi>
Date: 3 Jan 93 10:54:51 GMT
Sender: daemon@net.bio.net
Distribution: bionet
Lines: 497


>Path: funic!fuug!mcsun!uunet!tacom-emh1.army.mil!w8sdz
>From: w8sdz@tacom-emh1.army.mil (Keith Petersen)
>Newsgroups: comp.archives.msdos.announce
>Subject: msdos uploads to SIMTEL20 during the month of December, 1992
>Summary: 275 new files were uploaded
>Keywords: simtel20,archives,msdos,monthly,uploads
>Message-ID: <9301030731.kp16868@tacom-emh1.army.mil>
>Date: Sun, 3 Jan 1993 07:31:46 GMT
>Followup-To: comp.archives.msdos.d
>Organization: The SIMTEL20 Archives
>Approved: w8sdz@tacom-emh1.army.mil
>Lines: 483

File PD1:<MSDOS.FILEDOCS>UPLOADS.DEC                 Created: Jan. 3, 1993

NOTE: This file is also available in comma-delimited format as SIM9212.IDX

           MSDOS FILES UPLOADED TO WSMR-SIMTEL20.ARMY.MIL
                  during the month of December 1992

NOTE: Type B is Binary; Type A is ASCII

 Filename   Type Length   Date    Description
==============================================
Directory PD1:<MSDOS.4DOS>
4FILE2U6.ZIP  B   75433  921203  4DOS file management tool & description editor
4UTILS7.ZIP   B   96374  921219  4DOS description editor & file finder (free)
EZ-BTM05.ZIP  B   50180  921231  4DOS BTM files: loaders/regular/libraries/help

Directory PD1:<MSDOS.ARC-LBR>
ARCE41A.ZIP   B    9224  921207  Vernon Buerg's fast ARC extraction program
HPACK78.ZIP   B  113973  921231  High performance archiver from New Zealand
SHEZ83.ZIP    B  211003  921227  Shell for archive manipulation, w/virus check

Directory PD1:<MSDOS.ASTRONOMY>
COSMOS16.ZIP  B  178908  921229  SVGA to Herc, professional Astronomy simulator

Directory PD1:<MSDOS.AUTOCAD>
ACADPCB.ZIP   B   71268  921223  Tools and blocks for PC board design w/AutoCAD
STREKDXF.ZIP  B  297704  921223  DXF format of Enterprise drawing for Autocad

Directory PD1:<MSDOS.BATUTL>
STRINGS2.ZIP  B   82478  921207  Adds 70 string handling functions to BAT files
TSBAT40.ZIP   B   96390  921219  Collection of useful batch files by Timo Salmi

Directory PD1:<MSDOS.BBS>
PBINSBAT.ZIP  B    1807  921207  Batch file to install Powerboard BBS v1.20
PCBURM13.ZIP  B  130308  921217  PCBoard BBS user file/byte ratio monitor v1.3
SBBS_116.ZIP  B  777831  921202  SuperBBS: QBBS-compatible multi-node BBS,v1.16
TOPFILES.ZIP  B  134095  921227  PC Board top files d'loaded during last x days

Directory PD1:<MSDOS.BIBLE>
GWW11A01.ZIP  B  333509  921208  Complete Bible search prog. for Windows, 1/12
GWW11A02.ZIP  B  360810  921208  Complete Bible search prog. for Windows, 2/12
GWW11A03.ZIP  B  360810  921208  Complete Bible search prog. for Windows, 3/12
GWW11A04.ZIP  B  360810  921208  Complete Bible search prog. for Windows, 4/12
GWW11A05.ZIP  B  330096  921208  Complete Bible search prog. for Windows, 5/12
GWW11A06.ZIP  B  360810  921208  Complete Bible search prog. for Windows, 6/12
GWW11A07.ZIP  B  360810  921208  Complete Bible search prog. for Windows, 7/12
GWW11A08.ZIP  B  360810  921208  Complete Bible search prog. for Windows, 8/12
GWW11A09.ZIP  B  360810  921208  Complete Bible search prog. for Windows, 9/12
GWW11A10.ZIP  B  360810  921208  Complete Bible search prog. for Windows, 10/12
GWW11A11.ZIP  B  295595  921208  Complete Bible search prog. for Windows, 12/12
GWW11A12.ZIP  B  106869  921208  Complete Bible search prog. for Windows, 12/12

Directory PD1:<MSDOS.BIOLOGY>
BED100.ZIP    B   60715  921215  Molecular Biology input feedback echo w/speakr

Directory PD1:<MSDOS.BOOKS>
ANNE10.ZIP    B  244863  921202  Nov 1992 Anne of Green Gables

Directory PD1:<MSDOS.BORLAND>
PRBGI097.ZIP  B  357003  921215  BGI driver for dot matrix & laserjet printers

Directory PD1:<MSDOS.C>
SPWNO413.ZIP  B  107266  921212  Disk/EMS/EXT/INT15-swapping spawn() replacemnt

Directory PD1:<MSDOS.CAD>
KNWRS120.ZIP  B  139541  921227  KnitWare: Sweater knitting design/pattern prnt

Directory PD1:<MSDOS.CPLUSPLUS>
JCOOL01.ZIP   B  441547  921202  Extensive C++ class library (uses templates)
NEWMAT06.ZIP  B  108322  921203  C++ classes and functions for Matrices

Directory PD1:<MSDOS.CROSSASM>
ASSEMBLR.ZIP  B   54456  921210  Generic 6502/6803/8085 assembler

Directory PD1:<MSDOS.DATABASE>
CCHEF20.ZIP   B  186624  921219  CompuChef v2.0: Full-featured recipe database
CDS24D.ZIP    B   52587  921207  CD Catalog with browse & search. Mouse Support
MM-703.ZIP    B  207319  921207  MealMaster database program for recipes, v7.03
POS410.ZIP    B  310413  921202  ESD Point of Sale with Accounts Receivable
SCHED203.ZIP  B  365313  921204  Automatic employee scheduling system. DEMO
SSDINF11.ZIP  B   16133  921227  Displays information on selected SAS Datasets
TAPEM16B.ZIP  B   67967  921229  Music database & audio cassette label maker
TS20.ZIP      B  947840  921227  Travel Search: Hotels, resorts, inns in U.S.A.

Directory PD1:<MSDOS.DBASE>
LIB19.ZIP     B  211130  921223  dBASE IV procedure/UDF library

Directory PD1:<MSDOS.DDJMAG>
DDJ9210.ZIP   B 1603338  921231  Dr. Dobbs Journal mag listings, Oct. 1992
DDJ9211.ZIP   B  276723  921216  Dr. Dobbs Journal mag listings, Nov. 1992
DDJ9212.ZIP   B 1445216  921216  Dr. Dobbs Journal mag listings, Dec. 1992
DDJ9301.ZIP   B  403092  921216  Dr. Dobbs Journal mag listings, Jan. 1993

Directory PD1:<MSDOS.DESKACCESS>
FAC171.ZIP    B  120787  921207  Fast fuzzy find US/foreign telephone area code

Directory PD1:<MSDOS.DESKPUB>
EVP10A.ZIP    B  354479  921202  EnVision Publisher: DTP GUI,WYSIWYG,mouse,1of2
EVP10B.ZIP    B  314934  921202  EnVision Publisher: DTP GUI,WYSIWYG,mouse,2of2

Directory PD1:<MSDOS.DESQVIEW>
DVFAQ10.ZIP   B   72696  921207  DESQview/QEMM Frequently Asked Questions

Directory PD1:<MSDOS.DIRUTL>
OBSLT10.ZIP   B    7661  921227  Recursive delete files older than selected age
QDEL032.ZIP   B   22708  921221  Del dir tree FAST. Options, fun, statistics
RMYG13.ZIP    B    5419  921227  Joseph Gil's tiny Unix-like file removal util.
WALKDIR.ZIP   B   10998  921218  Walk through directory structure & print info

Directory PD1:<MSDOS.DISASM>
DASM85.ZIP    B   11084  921210  Disassembler for 8085 binaries
DMPPRG20.ZIP  B   46604  921202  Disassembles MS-DOS & Windows 3 .EXE files

Directory PD1:<MSDOS.DSKUTL>
COMBI110.ZIP  B   41443  921201  RAM disk & disk cache sharing XMS memory
COPYD11.ZIP   B   40075  921207  Wsscopyd, copy/format disks, 1-pass, w/source
FFIT162.ZIP   B   42303  921209  Copies files to disks optimally (knapsack)
HSUTIL60.ZIP  B  141554  921202  Hughes-Schoolfield collection of DOS utilities
JUMBO303.ZIP  B  322305  921228  CMS 120MB and 250MB tape drive software v3.03
MBACK210.ZIP  B  225252  921207  Patri-Soft's comprehensive disk system backup
QCOPY40.ZIP   B   80267  921207  QCOPY v4.0: Disk copying and format utility
SPKT451S.ZIP  B  291958  921207  HyperDisk disk cache w/HyperKey & HyperScreen

Directory PD1:<MSDOS.DV-X>
DCLOCK.ZIP    B  159082  921208  Simple digital alarm clock for DESQview/X
DVIX.ZIP      B  105798  921208  DVI previewer for DESQview/X
FNVW100.ZIP   B  155632  921202  DESQview/X font viewer, v1.00
GS252DVX.ZIP  B 1331344  921208  Postscript interpreter that runs under DV/X
TGIF212.ZIP   B  950862  921208  Xlib-based draw program that runs under DVX
XCALC.ZIP     B  179536  921208  Scientific calculator (TI-30 / HP-10C) for DVX
XCOLORED.ZIP  B  199169  921208  Graphical tool for mixing colors under DVX
XCOLORMA.ZIP  B  124690  921208  Displays current default colormap under DVX

Directory PD1:<MSDOS.EDITOR>
ANSIPC10.ZIP  B   74989  921218  ANSI graphics editor w/extensive mouse support
BE300.ZIP     B  236741  921219  Bingo: Multi-window programmer's text editor
DNGDNG11.ZIP  B  323790  921204  Chinese Word Processor (previously CEDIT)
KANTKS10.ZIP  B  107481  921207  Kannada Script editor/Transliterator, req. EGA
ME600.ZIP     B  554035  921215  Multi-Edit v6.0: Programmer's text editor
ME_CD25.ZIP   B  357600  921202  ME2 v2.5: Extendable emacs-like text editor
TRIVED08.ZIP  B   12532  921207  FOSSIL/console vi-like editor for BBS use

Directory PD1:<MSDOS.EDUCATION>
GSH220.ZIP    B  391784  921201  Gradebook program for college/university
MATHSP11.ZIP  B   37188  921218  Mathshop: A game-like math drill for age 6-12
PHYSCSXA.ZIP  B  293817  921203  Physics tutorial, graphic oriented hypertext
SUDBURY.ZIP   B  718539  921208  Learn mining vocabulary and French grammar

Directory PD1:<MSDOS.ELECTRICAL>
ADC8_10.ZIP   B   62706  921207  How: 8 bit analog->digital cvtr w/printr port
SPCTR3E2.ZIP  B  364462  921227  386/486 vers. of SPICE 3e2 ckt simu
From owner-software@net.bio.net Sat Jan 02 22:00:00 1993
Path: biosci!ICBR.IFAS.UFL.EDU!SJJ
From: SJJ@ICBR.IFAS.UFL.EDU ("JONG, SONG-MUH J")
Newsgroups: bionet.software
Subject: AsEasyAs spreadsheet
Message-ID: <9301031726.AA14971@net.bio.net>
Date: 3 Jan 93 17:17:00 GMT
Sender: daemon@net.bio.net
Distribution: bionet
Lines: 12

Dear netters,

I am writing regarding the graphic functions of the spreadsheet AsEasyAs.
This program has been selected as one of the best in MSDOS sharewares.
However, I found it difficult to create simple graphs with numbers ranging
from positive to negative numbers, or numbers with decimals. Can anyone
enlighten me as to the ways of doing graphics in AsEasyAs? Or perhaps
the graphics functions are only available from the high-end products?

Song-Muh Jong
sjj@icbr.ifas.ufl.edu


From owner-software@net.bio.net Sat Jan 02 22:00:00 1993
Path: biosci!foxmail.gfc.edu!dpowers
From: dpowers@foxmail.gfc.edu (Donald R. Powers)
Newsgroups: bionet.software
Subject: LabView hardware
Message-ID: <1993Jan02.165323.24056@foxmail.gfc.edu>
Date: 3 Jan 93 00:53:23 GMT
Sender: daemon@net.bio.net
Distribution: bionet
Organization: George Fox College, Newberg, Oregon USA
Lines: 11

I have LabView II and would like to set it up to monitor several
instruments on my Mac IIci.  Can anyone suggest a reasonably priced A/D
device I can use for an interface?  Most of what I have involves a
simple voltage inpute.  However, it is possible I might need a
differential input at some point.  Thanks!

Don Powers
*********************************************************************
This message was created on the George Fox College Fox Mail system.
The views expressed are those of the individual author only.
*********************************************************************

From owner-software@net.bio.net Sun Jan 03 22:00:00 1993
Path: biosci!agate!doc.ic.ac.uk!daresbury!buzz.bmc.uu.se!embl-heidelberg.de!omond
From: omond@embl-heidelberg.de
Newsgroups: bionet.software
Subject: Re: Protein Structure Prediction mailserver
Message-ID: <1993Jan4.152545.62019@embl-heidelberg.de>
Date: 4 Jan 93 14:25:45 GMT
References: <1993Jan2.173434.1@vax.path.ox.ac.uk>
Organization: EMBL, European Molecular Biology Laboratory
Lines: 27

In article <1993Jan2.173434.1@vax.path.ox.ac.uk>, jasper@vax.path.ox.ac.uk writes:

> 	I haven't *seen* this posted anywhere yet (apologies if it has been,
> I have that sneaking suspicion we are losing some articles through the feed)
> but the information is tucked away in Nature, so I think it needs advertising!
>
> ****  PHD - a new mail server for protein secondary structure prediction  ***
>
> The return times are reasonably fast, but maybe that's because I'm testing it
> in the vacation? Overall round trip time for a 200 amino acid domain is about
> 30 minutes. Of course this could get a lot slower if everyone tries it at
> once!

Jasper, sorry to revert to posting to the net, but ... you might be pleasantly
surprised to get a faster turnround if you configure your machine 
vax.path.ox.ac.uk to actually ACCEPT e-mail connections.  We've got lots
of stuff in our mail queue for you guys, and your machine constantly rejects
SMTP connection request from us.  Actually this seems to be a common "feature"
of sites in Oxford, since it happens pretty regularly.  What mailer software
are you running ?

Regards,

Roy Omond
System Manager/PostMaster etc. etc.
European Molecular Biology Laboratory
Heidelberg, Germany.

From owner-software@net.bio.net Sun Jan 03 22:00:00 1993
Path: biosci!uwm.edu!cs.utexas.edu!sdd.hp.com!think.com!yale.edu!ira.uka.de!chx400!bernina!neptune!gonnet
From: gonnet@inf.ethz.ch (Gaston Gonnet)
Newsgroups: bionet.software
Subject: Re: Program to compare all members of a group of sequences
Message-ID: <1993Jan4.102845.16334@neptune.inf.ethz.ch>
Date: 4 Jan 93 10:28:45 GMT
References: <1992Dec30.051200.21456@tigger.jvnc.net>
Sender: news@neptune.inf.ethz.ch (Mr News)
Organization: Dept. Informatik, Swiss Federal Institute of Technology (ETH), Zurich, CH
Lines: 21
Nntp-Posting-Host: rutishauser-gw.inf.ethz.ch

In article <1992Dec30.051200.21456@tigger.jvnc.net> mrl775@tigger.jvnc.net writes:
> Hello
>
>Does anyone know of a program that will take a set of sequences, and find
>common local alignments (sort of like what blast and blast3 do, but with each
>member of the set).  I am looking to define cofactor binding sites, and have a
>list of sequences that all bind the cofactor, but have no good method of
>comparing them all together (a multiple alignment doesnt work).  Any hints,
>pointers, source, executables would be appreciated.
======================================================
We compute multiple alignments based on local (all-against-all)
alignments.  This may solve your problem.  You can try this by
submitting a request to our automatic server.

Send a mail message to  cbrg@inf.ethz.ch  with the body

help AllAll

and you will receive a description on how to submit requests.

Gaston H. Gonnet, Informatik, Zurich

From owner-software@net.bio.net Mon Jan 04 22:00:00 1993
Path: biosci!daresbury!daresbury!news
From: PARSONS_A@snd01.pcr.co.uk
Newsgroups: bionet.software
Subject: GCG synchronisation problem
Message-ID: <1993Jan5.093228.21154@gserv1.dl.ac.uk>
Date: 5 Jan 93 09:33:09 GMT
Sender: list-admin@daresbury.ac.uk
Distribution: bionet
Lines: 166
Original-To: BIO-SOFT <BIO-SOFT@UK.AC.DARESBURY>

Happy New Year Neteers,

I have had no joy out of GCG on this one - anyone had anyexperience of this 
particular error whilst using GCG and (a) what it might mean and (b) how it
affects the quality of the results that I get?

Thanks in Advance,

Tony Parsons <<parsons_a@snd01.pcr.co.uk = Pfizer Central Research UK>>

Details.....

I was trying to search PIR using FASTA and got the following error :-

 *** ERROR in SQNext. Sequence reading is out of synchronization. 


Any ideas what is going on?

Log file follows...

Tony Parsons - Pfizer central Research  (parsons_a@snd01.pcr.co.uk)

-------------------------  log of session  -----------------------------------

 		                WELCOME TO GCG
                      Sequence Analysis Software Package 
                           Version 7.1,  March, 1992

         (c)1982, 1983, 1984, 1985, 1986, 1987, 1989, 1991, 1992
              Genetics Computer Group, Inc.  All rights reserved.

         Published research assisted by this software should cite:
  Devereux, Haeberli and Smithies (1984). A Comprehensive Set of Sequence 
    Analysis Programs for the VAX. Nucleic Acids Research 12(1); 387-395 

Databases available: 
GenBank            Release 73.0 (9/92)  PIR-Protein        Release 34.0 (10/92)
EMBL (Modified)    Release 32.0 (9/92)  SwissProt          Release 23.0 ( 8/92)
PIR-Nucleic        Release 36.0 (3/90)  Vecbase            Release  3.0 ( 8/87)
PROSITE            Release  9.2 (9/92)  REBASE restriction enzymes 9210 (10/92)
			MIPSX  34.0  (9/92)
             Help is available with the command $ GenHelp

PROBLEMS WITH NEW SOFTWARE or DATABASES        email: PARSONS_A   tel - 6871
 Running Fasta  using : 
...
/INfile=ul08.gcg
/BEGin=1
/END=750
/INfile2=p:*
/WORd=2
/LIStsize=40
/OUTfile=Ul08.Fasta
/Default


 *** ERROR in SQNext. Sequence reading is out of synchronization. 

 



(Peptide) FASTA of: Ul08.Gcg  from: 1   to: 750  December 8, 1992  15:09
 TO: p:*  Sequences:        223  Symbols:     30,658  Word Size: 2


Score Init1 Initn
<  2     0     0:
   4     0     0:
   6     0     0:
   8     0     0:
  10     0     0:
  12     0     0:
  14     0     0:
  16     1     1:=
  18     4     4:==
  20    12    12:======
  22    26    26:=============
  24    21    21:===========
  26    35    35:==================
  28    30    30:===============
  30    10    10:=====
  32    44    43:======================
  34     7     6:===-
  36     4     2:=-
  38    10     6:===--
  40     7     3:==--
  42     4     5:==+
  44     2     2:=
  46     1     1:=
  48     5     6:===
  50     0     2:+
  52     0     1:+
  54     0     1:+
  56     0     1:+
  58     0     0:
  60     0     1:+
  62     0     1:+
  64     0     0:
  66     0     1:+
  68     0     1:+
  70     0     0:
  72     0     1:+
  74     0     0:
  76     0     0:
  78     0     0:
  80     0     0:
> 80     0     0:
 mean initn score:  23.8 (2.83)
 mean init1 score:  23.8 (2.83)


The best scores are:				            init1 initn opt

Pir1:Cbms  Cytochrome b - Mouse mitochondrion (SGC1)          39    72    40
Pir1:Cbrt  Cytochrome b - Rat mitochondrion (SGC1)            39    68    40
Pir1:Cbqfr  Cytochrome b - Rhodospirillum rubrum              39    66    41
Pir1:Cbhu  Cytochrome b - Human mitochondrion (SGC1)          37    62    39
Pir1:Wmrz17  Cytochrome b6-f complex 17K protein - Rice c...  44    59    66
Pir1:O4hud1  Debrisoquine 4-hydroxylase (EC 1.14.14.-) cy...  37    55    37
Pir1:Cbbo  Cytochrome b - Bovine mitochondrion (SGC1)         39    54    40
Pir1:Wmlv17  Cytochrome b6-f complex 17K protein - Liverw...  37    52    57
Pir1:Cbxl  Cytochrome b - African clawed frog mitochondri...  38    49    42
Pir1:Ccqfct  Cytochrome c' - Rhodocyclus tenuis               35    49    35
Pir1:Cbboc6  Cytochrome b-c1 complex, 6.4K protein - Bovine   36    48    41
Pir1:Cblv55  Cytochrome b559 component E - Liverwort (Mar...  47    47    50
Pir1:Cbobe  Cytochrome b - Evening primrose mitochondrion     47    47    61
Pir1:Cbnt55  Cytochrome b559 component E - Common tobacco...  47    47    50
Pir1:Cbrz55  Cytochrome b559 component E - Rice chloroplast   47    47    50
Pir1:Cbvf  Cytochrome b - Fava bean mitochondrion             47    47    61
Pir1:Wmnt17  Cytochrome b6-f complex 17K protein - Common...  45    45    67
Pir1:Ccrfpp  Cytochrome c' - Rhodobacter capsulatus           33    43    33
Pir1:Cclm  Cytochrome c - Pacific lamprey                     43    43    46
Pir1:Cbutb  Cytochrome b - Trypanosoma brucei mitochondri...  32    42    42
Pir1:Rdbyun  Ubiquinol--cytochrome-c reductase (EC 1.10.2...  42    42    42
Pir1:Wmsp17  Cytochrome b6-f complex 17K protein - Spinac...  41    41    67
Pir1:Cbyb55  Cytochrome b559 component psbE - Synechocyst...  41    41    48
Pir1:O4psz  Denitrification system component nirT - Pseud...  41    41    51
Pir1:Ccps5a  Cytochrome c551 - Pseudomonas aeruginosa         40    40    41
Pir1:Cbrz  Cytochrome b - Rice mitochondrion                  39    39    41
Pir1:Cbzm  Cytochrome b - Maize mitochondrion                 39    39    41
Pir1:Ccps5f  Cytochrome c551 - Pseudomonas fluorescens bi...  38    38    49
Pir1:Cbbo5  Cytochrome b5 - Bovine                            38    38    52
Pir1:Ccps5d  Cytochrome c551 - Pseudomonas sp.                38    38    49
Pir1:Ccbf6  Cytochrome c6 - Yellow-green alga (Bumillerio...  37    37    39
Pir1:Ccps5s  Cytochrome c551 - Pseudomonas stutzeri (stra...  37    37    52
Pir1:Ccps5b  Cytochrome c551 precursor - Pseudomonas stut...  37    37    47
Pir1:Wmkl17  Cytochrome b6-f complex 17K protein - Chlore...  36    36    38
Pir1:Cctw5t  Cytochrome c552 - Thermus aquaticus              36    36    36
Pir1:Cblv6  Cytochrome b6 - Liverwort (Marchantia polymor...  34    34    36
Pir1:Ccck  Cytochrome c - Yeast (Issatchenkia orientalis)     33    33    36
Pir1:Ccus  Cytochrome c - Smut fungus (Ustilago sphaerogena)  33    33    34
Pir1:Cceg  Cytochrome c - Euglena gracilis                    33    33    41
Pir1:Cchq  Cytochrome c - Yeast (Hansenula anomala)           33    33    37

Aligning...
 CPU time:  0:00:19
 Output File: Ul08.Fasta
  PARSONS_A    job terminated at  8-DEC-1992 15:13:16.77

  Accounting information:
  Buffered I/O count:             457         Peak working set size:   12603
  Direct I/O count:              1919         Peak page file size:     21702
  Page faults:                  15293         Mounted volumes:             0
  Charged CPU time:           0 00:00:29.73   Elapsed time:     0 00:05:30.12

From owner-software@net.bio.net Mon Jan 04 22:00:00 1993
Path: biosci!bcm!cs.utexas.edu!sdd.hp.com!usc!wupost!spool.mu.edu!agate!usenet.ins.cwru.edu!po.CWRU.Edu!ccy
From: ccy@po.CWRU.Edu (Cheung C. Yue)
Newsgroups: bionet.software
Subject: Ethernet card recommendations for MacII
Message-ID: <1iaoi4INN3vf@usenet.INS.CWRU.Edu>
Date: 5 Jan 93 01:33:56 GMT
Reply-To: ccy@po.CWRU.Edu (Cheung C. Yue)
Organization: Case Western Reserve University, Cleveland, OH (USA)
Lines: 12
NNTP-Posting-Host: hela.ins.cwru.edu


I am daydreaming again, hoping to put the Mac IIsi in my laboratory
on the net.  Not being particularly familiar with Mac's, I would
like to solicit recommendations for brands of ethernet cards.  Also
I would welcome suggestions of appropriate software (I suppose NCSA
Telnet is probably the best).  Finally I wonder if it is possible to
allow remote control of the Mac over ethernet.  Thanks in advance for
all your input.

C Cho Yue
ccy@po.cwru.edu
-- 

From owner-software@net.bio.net Mon Jan 04 22:00:00 1993
Path: biosci!bcm!cs.utexas.edu!uwm.edu!spool.mu.edu!uunet!pipex!demon!manor.demon.co.uk!paulf
From: paulf@manor.demon.co.uk (Paul Fawcett)
Newsgroups: comp.ai,comp.ai.neural-nets,sci.cognitive,comp.theory.cell-automata,bionet.neuroscience,bionet.molbio.evolution,bionet.software
Subject: Biologically Plausible Dynamic Artificial Neural Networks
Message-ID: <726213237snz@manor.demon.co.uk>
Date: 5 Jan 93 05:53:57 GMT
Sender: usenet@demon.co.uk
Reply-To: paulf@manor.demon.co.uk
Organization: UDI
Lines: 59
Xref: biosci comp.ai:1368 comp.ai.neural-nets:1257 sci.cognitive:692 comp.theory.cell-automata:204 bionet.neuroscience:872 bionet.molbio.evolution:716 bionet.software:3875
X-Mailer: Simple NEWS 1.90 (ka9q DIS 1.19)


          Biologically  Plausible  Dynamic Artificial Neural Networks.
          -----------------------------------------------------------

          A   *Dynamic   Artificial   Neural   Network*   (DANN)   [1]
          possesses   processing  elements  that  are  created  and/or
          annihilated,  either in real time  or  as  some  part  of  a
          development phase [2].

          Of    particular    interest    is    the   possibility   of
          constructing   *biologically    plausible*    DANN's    that
          models    developmental   neurobiological   strategies   for
          establishing  and   modifying processing elements and  their
          connections.

          Work  with  cellular  automata in modeling cell genesis  and
          cell pattern  formation  could  be applicable to the  design
          of  DANN topologies.  Likewise, biological features that are
          determined by genetic  or  evolutionary  factors  [3]  would
          also have a  role  to play.

          Putting  all  this  together  with  a view to constructing a
          working DANN,  possessing cognitive/behavioral attributes of
          a biological system is a tall order; the modeling of nervous
          systems in simple organisms may be the  best  approach  when
          dealing with a problem of such complexity [4].

          Any  comments,  opinions  or  references  in respect of  the
          above assertions would be most welcome.


          Many thanks

          Paul Fawcett.

          University of Westminster


          References.

          1. Ross, M. D., et al  (1990);  Toward  Modeling  a  Dynamic
             Biological   Neural   Network,  Mathl  Comput.  Modeling,
             Vol 13 No.7, pp97-105.

          2. Lee, Tsu-Chang,(1991);  Structure  Level  Adaptation  for
             Artificial  Neural  Networks, Kluwer Academic Publishers.

          3. Edleman,  Gerald,(1987);  Neural Darwinism the Theory of
             Neural Group Selection, Basic Books.

          4. Beer, Randal, D,(1990); Intelligence as Adaptive Behavior
             :  An   Experiment   in    Computational   Neuroethology.
             Academic  Press.

-- 
--------------------------------------------------------------------------
Paul Fawcett                 |      Internet: paulf@manor.demon.co.uk
London, UK.                  |                tenec@westminster.ac.uk
--------------------------------------------------------------------------  

From owner-software@net.bio.net Mon Jan 04 22:00:00 1993
Path: biosci!daresbury!buzz.bmc.uu.se!corax.udac.uu.se!sunic!mcsun!uunet!spool.mu.edu!caen!batcomputer!ghost.dsi.unimi.it!univ-lyon1.fr!chx400!news.unige.ch!pun
From: pun@cui.unige.ch (PUN Thierry)
Newsgroups: bionet.software
Subject: Re: Analysis of 1-D Gels
Keywords: PAGE, AGAROSE GEL ANALYSIS
Message-ID: <1993Jan5.081627.24635@news.unige.ch>
Date: 5 Jan 93 08:16:27 GMT
References: <1993Jan5.065024.15769@hippo.ru.ac.za>
Sender: usenet@news.unige.ch
Organization: University of Geneva, Switzerland
Lines: 193

In article <1993Jan5.065024.15769@hippo.ru.ac.za> mimq@hippo.ru.ac.za (Molapo Qhobela) writes:
>
>Can anyone out there help me out. I am looking for a DOS based software
>package that can be used to analyse 1-D gels, with the ultimate aim of
>perantage determination of samples. I currently use a package called
>GelManager by Peter Jackman, but it only calculates similarities between
>samples. I saw a similar posting on this subject a few months back, but
>I never saw the responses.
>

Hello,

Our software LaboImage (public domain, general purpose image analysis,
see doc below) includes a comprehensive 1D gel analysis program. This
program is based on work I performed at the NIH in 1982-1985, but has 
been rewritten for Sun, then for Motif/X11. 

We have not had the resources to run LaboImage on PC/DOS, but since it
is now based on Motif/X11, it is feasible.

Best regards,

     Prof. Thierry Pun, Computer Vision Group
     Computing Science Center, University of Geneva
     24, r. General Dufour, CH-1211 Geneva 4, SWITZERLAND  
     Phone : +41(22) 705 7627; secr: x7660; fax: x7780
     E-mail: pun@cui.unige.ch [or pun@cgeuge51.bitnet]




********************************************************************************

LaboImage
---------
Original notice

March 8th, 1989: LaboImage 2.0 (SunView)
August 24th, 1990: LaboImage 3.0 (SunView)
March 19th, 1991: LaboImage 3.1 (SunView)
December 1st, 1992: LaboImage 4.0 (X11 / OSF Motif)
Computer Science Center, University of Geneva, Switzerland

Thank you for your interest in LaboImage!


General description
-------------------
Labo Image is a window based software for image processing and analysis. It
contains a comprehensive set of operators as well as general utilities. It
is designed to be open-ended; new modules can easily be added. The software,
written in C, is now based on X11 / OSF Motif. The current version has been
developped and tested on a Sun SPARC station, with X11r4 and Motif 1.1.
LaboImage has been extensively used by students as well as researchers from
various domains: computer science (image analysis), medicine, biology, physics.
It is distributed free of charge (source code).

Status
------
Version 4.0, 1st December 1992:

    - hosts: Sun SPARC station;
    - OS: 4.1.1;
    - window system: X11r4 / Motif 1.1;
    - language: C;
    - approx. code size: source 2.5MB (80'000 lines), executable 2.5MB;
    - documentation: interactive help (english)

Means of distributions
----------------------

LaboImage source code is available by anonymous ftp at:
1) ftp.ads.com, login name anonymous, in pub/VISION-LIST-ARCHIVE/SHAREWARE.
2) peipa.essex.ac.uk, login name anonymous, in ipa/proc-src.
If you have no access to ftp, please contact the author.
If you wish to be kept current with update, error reports, ..., please send
us a mail with your full name, regular and electronic addresses.

Distribution policy
-------------------
In essence:
    - this is a non-profit software, but it is our property and the copyright
      notice must appear;
    - the reference to cite in case of published results obtained with Labo
      Image is: "A. Jacot-Descombes, M. Rupp, T. Pun: `LaboImage: A portable
      window-based environment for research in image processing and analysis',
      SPIE Symposium on Electronic Imaging Science and Technology, Image
      Processing: Implementation and Systems, San Jose, California, USA,
      Feb. 9-14, 1992.
    - no responsibility is assumed;
    - not to be used for profit making purposes;
    - bugs will usually be corrected since we use the software intensively ;
    - modifications should be communicated to us, with (normally) allowance
      for redistribution.

Payment
-------
Athough LaboImage has undergone many upgrades and suffered in the hands of 
many users, the current version is certainly not bug free. For the time being,
we require NO prepayment, return postage or anything. 
We may however change this policy in the future, and ask for nominal fees to
cover material expenditures. HOWEVER, if you are satisfied with the product,
why not send us some "souvenir" (post card, drink, etc.)) from your country...??!!

Capabilities
------------
Labo Image is an interactive software, whose interface is menu, mouse and 
window based. Its main features are:
    - input-output: LaboImage format file, SUN raster file; postscript;
    - display: mono, RGB, dithering, color table editor;
    - preprocessing: filters (median, high pass, low pass: hamming, gauss, etc),
	    background subtraction, histogram equalization;
    - processing: thresholding, Fourier transforms, edge extractions: various
	    operators, ridge-riding, zero-crossing; segmentation into regions,
	    binary and gray tone mathematical morphology;
    - measures: histograms, image statistics, power, region outlining, 
	    object counting;
    - auxiliary: conversions, arithmetic & logical operations, noise addition,
	    image generation, magnification, convolution/correlation with
	    masks, image; padding;
    - elementary interactive operations: region outlining, statistics and
	    histogram computation, etc.;
    - special tools: 
	    - modify image at pixel level interactively,
	    - one-dimensional gel analysis,
	    - expert system for image segmentation (not implemented in LaboImage 4.0);
    - macros definitions, save and replay (not implemented in LaboImage 4.0);
    - on line documentation.

Image formats
-------------
Own format: descriptor file + data file (binary, byte, int, float, complex;
mono or RGB). Supports also Sun raster format. Conversions to various other 
formats.
Data structures:
    - iconic (pixel-based), with each image having its own parameter list;
    - vectors (histograms, look-up tables);

Miscellaneous remarks (answers to commonly asked questions)
---------------------

 - FILE FORMAT: we decided to go for: 1) a machine independant format; 2) a
   simple, data (ie. signal) oriented format. At the beginning of the
   development (summer 1987), we were not aware of any image format used by
   the whole community. There seems now to be some progress on the matter
   (TIFF, etc.), but they are still not that widely used in the community.
   Also, due to development priorities we consider conversion routines a more
   secondary issue as long as our format is simple. 
   In addition, the menu "ACQUISITION=>free byte format" is fairly versatile.
   Also, the SUN raster images can now be read into LaboImage and likewise
   images on system may be stored in SUN raster format.
   However.. we would welcome any software contribution!

 - 3D IMAGE PROCESSING: nothing special for such images.

 - ON LINE HELP: available.

Acknowledgements
----------------
More than 10 people have so far participated in this project, and their
contribution is gratefully acknowledged.
Staff: Pierre-Yves Burgi, Claudia Coiteux-Rosu, Ziping Hu, Alain Jacot-
Descombes, Rene Lutz, Christian Pellegrini, Thierry Pun, Marianne Logean-
Rupp, Krassimir Todorov.
Students: Anne Bobillier, Alain Brunner, Markus Buchi, Christian Girard,
Rene Perrier, Vrinda Shukla. 
Amongst them, A. Jacot-Descombes is responsible for general design issues, 
and is the keystone for implementation; R. Lutz is responsible for display
manipulations (Color Table Editor,etc.); T. Pun is responsible for the 
original layout and general design issues;
V. Shukla is responsible for the upgrade from LaboImage 2.0 to LaboImage 3.0;
Marianne Logean-Rupp is responsible for the portability of LaboImage to X11
(LaboImage 4.0).
We are particularly grateful to Drs. D. F. Hochstrasser and O. Ratib, Digital
Imaging Unit, Computer Center, University Hospital of Geneva, for their
extended support. LaboImage 4.0 could not have been without their help.

Contacts
--------
Particular problems will be redirected to relevant persons, but we prefer
that all communications be made to the same address:
e-mail: "pun@uni2a.unige.ch" or pun@cgeuge51.bitnet (if this fails,
	"pun@cui.unige.ch").
tel.: +(4122) 705 76 27 (T. Pun), 705 76 30 (A. Jacot-Descombes).
fax:  +(4122) 320 29 27.
postal address: Thierry Pun
                LaboImage
                Computing Science Center, University of Geneva
                24, rue du General-Dufour
                CH - 1211 Geneva 4
		SWITZERLAND

********************************************************************************

From owner-software@net.bio.net Mon Jan 04 22:00:00 1993
Path: biosci!s.u-tokyo!ccut!news.u-tokyo.ac.jp!wnoc-tyo-news!sh.wide!sun-barr!cs.utexas.edu!wupost!spool.mu.edu!uunet!psgrain!hippo!mimq
From: mimq@hippo.ru.ac.za (Molapo Qhobela)
Newsgroups: bionet.software
Subject: Analysis of 1-D Gels
Keywords: PAGE, AGAROSE GEL ANALYSIS
Message-ID: <1993Jan5.065024.15769@hippo.ru.ac.za>
Date: 5 Jan 93 06:50:24 GMT
Organization: Rhodes University, Grahamstown, South Africa
Lines: 20

Hi,

Can anyone out there help me out. I am looking for a DOS based software
package that can be used to analyse 1-D gels, with the ultimate aim of
perantage determination of samples. I currently use a package called
GelManager by Peter Jackman, but it only calculates similarities between
samples. I saw a similar posting on this subject a few months back, but
I never saw the responses.

Thanks in advance for your help. Please reply to this posting or
directly to me at mimq@hippo.ru.ac.za

Cheers

Molapo
-- 
   Molapo Qhobela PhD.                |  Do not ever get caught with 
   Rhodes University - Microbiology   |       your plants down!!
   Internet: mimq@hippo.ru.ac.za      |  Consult a Plant Pathologist 
   Telefax: +27 (0461) 24377          |            via APS 

From owner-software@net.bio.net Mon Jan 04 22:00:00 1993
Path: biosci!TIGGER.STCLOUD.MSUS.EDU!q00023
From: q00023@TIGGER.STCLOUD.MSUS.EDU
Newsgroups: bionet.software
Subject: want pkunpak program
Message-ID: <00966206.44C22B60.14459@TIGGER.STCLOUD.MSUS.EDU>
Date: 5 Jan 93 04:15:56 GMT
Sender: daemon@net.bio.net
Distribution: bionet
Lines: 7

Dear netters,
	I am looking for PKUNPAK program for de-arcing a program. Also I
amlooking for some useful list of biotechnoloy softwares. Could anyone can send
me the program and the list?

Thanks in advance,
Gabriel Cheung

From owner-software@net.bio.net Mon Jan 04 22:00:00 1993
Path: biosci!SALK-SC2.SDSC.EDU!mangalam
From: mangalam@SALK-SC2.SDSC.EDU (Harry Mangalam)
Newsgroups: bionet.software
Subject: Re: Mac plasmid programs?
Message-ID: <9301062004.AA02606@salk-sgi.sdsc.edu>
Date: 6 Jan 93 20:04:19 GMT
Sender: daemon@net.bio.net
Distribution: bionet
Lines: 65

Scott Monsma writes:
>Is anyone aware of Mac programs for keeping records of plasmid constructs?
>Such program would hopefully also draw graphic maps of the plasmids,
>and keep sequence information.  Thanks!

   As a matter of fact, yes....  Check out the program Gene Construction
Kit - an excerpt a critically disclaimed review follows (also check Peter
Markiewicz's reviews on the same (but older version of the) program.

Gene Construction Kit by Textco (vox/fax: 603 643 1471) is also a
relatively new entry but it is an extremely interesting cloning tool.  All
of the previously mentioned programs allow you to 'clone electronically',
but GCK is optimized for it (and does nothing else).  It is made only for
the Mac and while it may run on B+W Macs, it really requires a color
monitor to show its stuff.  It combines elements of a drawing program and
sequence editor to allow you to cut and paste elements of a number of
sequences together to form new constructs.  It remembers the history (even
multiple histories) of the sequences you use to clone, what enzymes you
used to clip the sequences, what you did to the ends to allow ligation (and
prevents you from performing biologically impossible operations); whatever
you create, you have a detailed construction history.  It now also allows
boolean searches of the associated text boxes in which you store any
comments.  In this way you could for instance, search all your "electronic
freezer" for any constructs that had a luciferase reporter gene AND amp AND
tet but NOT kan resistance genes.  
   Besides being a great idea, it is also extraordinarily easy to use.  The
last program that attempted this trick was a piece of work from
Intelligenetics called Stratagene (A great idea, if only they hadn't made
it for _only_ a $30,000 Xerox LISP workstation and demanded about $50,000
for the software).  Despite my aforementioned rage at demos, there is an
excellent demo-tutorial of GCK available at the usual archives
(ftp.bio.indiana.bio, in the IUBIO Software+Data/molbio/mac folder by
gopher).  
   There is also a new demo available direct from the company.  From my
experience with the new demo and its very reasonable cost (especially
compared to the amount of aggro and wasted time that a questionable
contruct can bring to a lab) there is no reason why any cloning lab should
be without this bit of software.  Surprisingly enough, it is also available
as a networked version, one of the few that is - however, Textco has taken
a somewhat contrary approach in pricing - they base their pricing not only
on the number of copies you buy (the more, the cheaper, as usual), but also
on the number of Macs that can be served (the larger the number, the more
expensive).  There is a logic to it, but it is not the usual logic 8). 
And, unlike the DNASTAR network license, GCK uses Keyserver, which allows
you find out how many keys are checked out, who has them and for how long. 
For a largish institution, this allows (evil word follows) recharging for
use, if anyone cares. 

   Later today, I'll upload the demo mentioned within to the Indiana (if
there's still room) and U Houston archives.  You can also get it direct
from our anonymous FTP server - FTP to salk-sc2.sdsc.edu, login as
anonymous, any password, get the 890K(!), binhexed, self extracting archive
named GCK_DEMO.SEA_HQX (it's a VAX, he said apologetically) in the
directory [anonymous.mac].

Cheers
Harry

Harry Mangalam                                   Vox:(619) 453-4100, x250
Dept of Biocomputing                                   Fax:(619) 552-1546
The Salk Institute                        1'   mangalam@salk-sc2.sdsc.edu
10010 N Torrey Pines Rd                   2'        hjm@salk-sgi.sdsc.edu
La Jolla CA 92037                         3'         mangalam@salk.bitnet



From owner-software@net.bio.net Mon Jan 04 22:00:00 1993
Path: biosci!snd01.pcr.co.uk!PARSONS_A
From: PARSONS_A@snd01.pcr.co.uk
Newsgroups: bionet.software
Subject: GCG synchronisation problem
Message-ID: <1993Jan5.093129.20898@gserv1.dl.ac.uk>
Date: 5 Jan 93 09:32:58 GMT
Sender: kristoff@net.bio.net
Distribution: bionet
Lines: 167

Happy New Year Neteers,

I have had no joy out of GCG on this one - anyone had anyexperience of this 
particular error whilst using GCG and (a) what it might mean and (b) how it
affects the quality of the results that I get?

Thanks in Advance,

Tony Parsons <<parsons_a@snd01.pcr.co.uk = Pfizer Central Research UK>>

Details.....

I was trying to search PIR using FASTA and got the following error :-

 *** ERROR in SQNext. Sequence reading is out of synchronization. 


Any ideas what is going on?

Log file follows...

Tony Parsons - Pfizer central Research  (parsons_a@snd01.pcr.co.uk)

-------------------------  log of session  -----------------------------------

 		                WELCOME TO GCG
                      Sequence Analysis Software Package 
                           Version 7.1,  March, 1992

         (c)1982, 1983, 1984, 1985, 1986, 1987, 1989, 1991, 1992
              Genetics Computer Group, Inc.  All rights reserved.

         Published research assisted by this software should cite:
  Devereux, Haeberli and Smithies (1984). A Comprehensive Set of Sequence 
    Analysis Programs for the VAX. Nucleic Acids Research 12(1); 387-395 

Databases available: 
GenBank            Release 73.0 (9/92)  PIR-Protein        Release 34.0 (10/92)
EMBL (Modified)    Release 32.0 (9/92)  SwissProt          Release 23.0 ( 8/92)
PIR-Nucleic        Release 36.0 (3/90)  Vecbase            Release  3.0 ( 8/87)
PROSITE            Release  9.2 (9/92)  REBASE restriction enzymes 9210 (10/92)
			MIPSX  34.0  (9/92)
             Help is available with the command $ GenHelp

PROBLEMS WITH NEW SOFTWARE or DATABASES        email: PARSONS_A   tel - 6871
 Running Fasta  using : 
...
/INfile=ul08.gcg
/BEGin=1
/END=750
/INfile2=p:*
/WORd=2
/LIStsize=40
/OUTfile=Ul08.Fasta
/Default


 *** ERROR in SQNext. Sequence reading is out of synchronization. 

 



(Peptide) FASTA of: Ul08.Gcg  from: 1   to: 750  December 8, 1992  15:09
 TO: p:*  Sequences:        223  Symbols:     30,658  Word Size: 2


Score Init1 Initn
<  2     0     0:
   4     0     0:
   6     0     0:
   8     0     0:
  10     0     0:
  12     0     0:
  14     0     0:
  16     1     1:=
  18     4     4:==
  20    12    12:======
  22    26    26:=============
  24    21    21:===========
  26    35    35:==================
  28    30    30:===============
  30    10    10:=====
  32    44    43:======================
  34     7     6:===-
  36     4     2:=-
  38    10     6:===--
  40     7     3:==--
  42     4     5:==+
  44     2     2:=
  46     1     1:=
  48     5     6:===
  50     0     2:+
  52     0     1:+
  54     0     1:+
  56     0     1:+
  58     0     0:
  60     0     1:+
  62     0     1:+
  64     0     0:
  66     0     1:+
  68     0     1:+
  70     0     0:
  72     0     1:+
  74     0     0:
  76     0     0:
  78     0     0:
  80     0     0:
> 80     0     0:
 mean initn score:  23.8 (2.83)
 mean init1 score:  23.8 (2.83)


The best scores are:				            init1 initn opt

Pir1:Cbms  Cytochrome b - Mouse mitochondrion (SGC1)          39    72    40
Pir1:Cbrt  Cytochrome b - Rat mitochondrion (SGC1)            39    68    40
Pir1:Cbqfr  Cytochrome b - Rhodospirillum rubrum              39    66    41
Pir1:Cbhu  Cytochrome b - Human mitochondrion (SGC1)          37    62    39
Pir1:Wmrz17  Cytochrome b6-f complex 17K protein - Rice c...  44    59    66
Pir1:O4hud1  Debrisoquine 4-hydroxylase (EC 1.14.14.-) cy...  37    55    37
Pir1:Cbbo  Cytochrome b - Bovine mitochondrion (SGC1)         39    54    40
Pir1:Wmlv17  Cytochrome b6-f complex 17K protein - Liverw...  37    52    57
Pir1:Cbxl  Cytochrome b - African clawed frog mitochondri...  38    49    42
Pir1:Ccqfct  Cytochrome c' - Rhodocyclus tenuis               35    49    35
Pir1:Cbboc6  Cytochrome b-c1 complex, 6.4K protein - Bovine   36    48    41
Pir1:Cblv55  Cytochrome b559 component E - Liverwort (Mar...  47    47    50
Pir1:Cbobe  Cytochrome b - Evening primrose mitochondrion     47    47    61
Pir1:Cbnt55  Cytochrome b559 component E - Common tobacco...  47    47    50
Pir1:Cbrz55  Cytochrome b559 component E - Rice chloroplast   47    47    50
Pir1:Cbvf  Cytochrome b - Fava bean mitochondrion             47    47    61
Pir1:Wmnt17  Cytochrome b6-f complex 17K protein - Common...  45    45    67
Pir1:Ccrfpp  Cytochrome c' - Rhodobacter capsulatus           33    43    33
Pir1:Cclm  Cytochrome c - Pacific lamprey                     43    43    46
Pir1:Cbutb  Cytochrome b - Trypanosoma brucei mitochondri...  32    42    42
Pir1:Rdbyun  Ubiquinol--cytochrome-c reductase (EC 1.10.2...  42    42    42
Pir1:Wmsp17  Cytochrome b6-f complex 17K protein - Spinac...  41    41    67
Pir1:Cbyb55  Cytochrome b559 component psbE - Synechocyst...  41    41    48
Pir1:O4psz  Denitrification system component nirT - Pseud...  41    41    51
Pir1:Ccps5a  Cytochrome c551 - Pseudomonas aeruginosa         40    40    41
Pir1:Cbrz  Cytochrome b - Rice mitochondrion                  39    39    41
Pir1:Cbzm  Cytochrome b - Maize mitochondrion                 39    39    41
Pir1:Ccps5f  Cytochrome c551 - Pseudomonas fluorescens bi...  38    38    49
Pir1:Cbbo5  Cytochrome b5 - Bovine                            38    38    52
Pir1:Ccps5d  Cytochrome c551 - Pseudomonas sp.                38    38    49
Pir1:Ccbf6  Cytochrome c6 - Yellow-green alga (Bumillerio...  37    37    39
Pir1:Ccps5s  Cytochrome c551 - Pseudomonas stutzeri (stra...  37    37    52
Pir1:Ccps5b  Cytochrome c551 precursor - Pseudomonas stut...  37    37    47
Pir1:Wmkl17  Cytochrome b6-f complex 17K protein - Chlore...  36    36    38
Pir1:Cctw5t  Cytochrome c552 - Thermus aquaticus              36    36    36
Pir1:Cblv6  Cytochrome b6 - Liverwort (Marchantia polymor...  34    34    36
Pir1:Ccck  Cytochrome c - Yeast (Issatchenkia orientalis)     33    33    36
Pir1:Ccus  Cytochrome c - Smut fungus (Ustilago sphaerogena)  33    33    34
Pir1:Cceg  Cytochrome c - Euglena gracilis                    33    33    41
Pir1:Cchq  Cytochrome c - Yeast (Hansenula anomala)           33    33    37

Aligning...
 CPU time:  0:00:19
 Output File: Ul08.Fasta
  PARSONS_A    job terminated at  8-DEC-1992 15:13:16.77

  Accounting information:
  Buffered I/O count:             457         Peak working set size:   12603
  Direct I/O count:              1919         Peak page file size:     21702
  Page faults:                  15293         Mounted volumes:             0
  Charged CPU time:           0 00:00:29.73   Elapsed time:     0 00:05:30.12


From owner-software@net.bio.net Mon Jan 04 22:00:00 1993
Path: biosci!bcm!cs.utexas.edu!milano!cactus.org!wixer!pacoid
From: pacoid@wixer.cactus.org (Paco Xander Nathan)
Newsgroups: bionet.software
Subject: Re: LabView hardware
Message-ID: <1993Jan5.014505.10138@wixer.cactus.org>
Date: 5 Jan 93 01:45:05 GMT
References: <1993Jan02.165323.24056@foxmail.gfc.edu>
Distribution: bionet
Organization: FringeWare Inc., +1 512 477 1366
Lines: 13

In article <1993Jan02.165323.24056@foxmail.gfc.edu> dpowers@foxmail.gfc.edu (Donald R. Powers) writes:
>I have LabView II and would like to set it up to monitor several
>instruments on my Mac IIci.  Can anyone suggest a reasonably priced A/D
>device I can use for an interface?  Most of what I have involves a
>simple voltage inpute.  However, it is possible I might need a
>differential input at some point.  Thanks!

Check with Radient in SF - 415 296 8040..  I wrote a review on them
for the last issue of Mondo 2000 - they have a CNX box that'll take
8 inputs into a Mac(A or D) and the whole system sells for around $350
which is a lot less than a LabView II ..  Tell 'em I sent you :-)

Paco Xander Nathan

From owner-software@net.bio.net Mon Jan 04 22:00:00 1993
Path: biosci!agate!usenet.ins.cwru.edu!magnus.acs.ohio-state.edu!zaphod.mps.ohio-state.edu!wupost!cs.utexas.edu!uunet!newsstand.cit.cornell.edu!vax5.cit.cornell.edu!samx
From: samx@vax5.cit.cornell.edu
Newsgroups: bionet.software
Subject: Mac plasmid programs?
Message-ID: <1993Jan5.114333.16686@vax5.cit.cornell.edu>
Date: 5 Jan 93 16:43:33 GMT
Distribution: bionet
Organization: Cornell University
Lines: 6

Is anyone aware of Mac programs for keeping records of plasmid constructs?
Such program would hopefully also draw graphic maps of the plasmids,
and keep sequence information.  Thanks!
	-Scott Monsma
	 Boyce Thompson Institute
	 607/254-1369

From owner-software@net.bio.net Mon Jan 04 22:00:00 1993
Path: biosci!daresbury!daresbury!news
From: PARSONS_A@snd01.pcr.co.uk
Newsgroups: bionet.software
Subject: RE: GCG Synchronisation Problem
Message-ID: <1993Jan5.145442.6246@gserv1.dl.ac.uk>
Date: 5 Jan 93 14:53:24 GMT
Sender: list-admin@daresbury.ac.uk
Distribution: bionet
Lines: 23
Original-To: BIO-SOFT <BIO-SOFT@UK.AC.DARESBURY>

Many thanks to all those who helped me out of a jam with a GCG
synchronisation problem in particular those at the foot of this
message - makes you wonder what we a re paying GCG maintenance for
sometimes.

Thanks again!

Rick Westerman                System Manager of the AIDS Center Laboratory
westerm@aclcb.purdue.edu      for Computational Biochemistry (ACLCB), BCHM
Phone: (317) 494-0505         bldg., Purdue University, W. Lafayette, IN 47907
  FAX: (317) 494-7897

Cary O'Donnell
*****************************************************************************
AFRC Computing Division    JANET   : ODONNELL@UK.AC.AFRC.ARCB
West Common                INTERNET: ODONNELL@ARCB.AFRC.AC.UK
Harpenden                  Long/Lat: 00d 21m 45s West   51d 48m 30s North
Herts AL5 2JE              Tel: (+44) 582 762271 xt 229 Fax: (+44) 582 761710
U.K.                       (AFRC = Agricultural & Food Research Council)
-----------------------------------------------------------------------------
|    Dr. Reinhard Doelz            | RFC     doelz@urz.unibas.ch         |
|      Biocomputing                | DECNET  20579::48130::doelz         |
|Biozentrum der Universitaet       | X25     022846211142036::doelz      |

From owner-software@net.bio.net Mon Jan 04 22:00:00 1993
Path: biosci!DARWIN.SFBR.ORG!bdyke
From: bdyke@DARWIN.SFBR.ORG (Bennett Dyke)
Newsgroups: bionet.software
Subject: Re: Ethernet card recommendations for MacII
Message-ID: <Pine.3.05.9301050818.A3629-b100000@darwin>
Date: 5 Jan 93 14:12:20 GMT
References: <9301050311.AA21058@net.bio.net>
Sender: daemon@net.bio.net
Distribution: bionet
Lines: 34

We have made it a practice to use external SCSI Ethernet boxes,
which are available from several vendors at about $275, with
all Macs without multiple internal slots.  Communications are
said to be a bit slower than with an internal card, but the
single slot of MacIIsi's is saved for other purposes, and we
have maximum flexibility to switch and upgrade processors.

+----------------------------------------------------------------------------+
|   Bennett Dyke                                                             |
|   Department of Genetics               Internet:  bdyke@darwin.sfbr.org    |
|   Southwest Foundation                    Phone:  (210) 674-1410 Ext 281   |
|   P.O. Box 28147                            Fax:  (210) 670-3317           |
|   San Antonio, TX 78228, USA                                               |
+----------------------------------------------------------------------------+


On 5 Jan 1993, Cheung C. Yue wrote:

> 
> I am daydreaming again, hoping to put the Mac IIsi in my laboratory
> on the net.  Not being particularly familiar with Mac's, I would
> like to solicit recommendations for brands of ethernet cards.  Also
> I would welcome suggestions of appropriate software (I suppose NCSA
> Telnet is probably the best).  Finally I wonder if it is possible to
> allow remote control of the Mac over ethernet.  Thanks in advance for
> all your input.
> 
> C Cho Yue
> ccy@po.cwru.edu
> -- 
> 




From owner-software@net.bio.net Mon Jan 04 22:00:00 1993
Path: biosci!daresbury!bioftp.unibas.ch!biox!doelz
From: doelz@comp.bioz.unibas.ch (Reinhard Doelz)
Newsgroups: bionet.software
Subject: Re: GCG synchronisation problem
Message-ID: <doelz.726229363@biox>
Date: 5 Jan 93 10:22:43 GMT
References: <1993Jan5.093228.21154@gserv1.dl.ac.uk>
Distribution: bionet
Organization: EMBnet Switzerland [Basel]
Lines: 135

PARSONS_A@snd01.pcr.co.uk writes:

>affects the quality of the results that I get?

This Problem is Lethal. 

>I was trying to search PIR using FASTA and got the following error :-

> *** ERROR in SQNext. Sequence reading is out of synchronization. 


>Any ideas what is going on?


You need to rerun dbindex and the problem should be fixed. 

Regards 
Reinhard 



> *** ERROR in SQNext. Sequence reading is out of synchronization. 

> 



>(Peptide) FASTA of: Ul08.Gcg  from: 1   to: 750  December 8, 1992  15:09
> TO: p:*  Sequences:        223  Symbols:     30,658  Word Size: 2


>Score Init1 Initn
><  2     0     0:
>   4     0     0:
>   6     0     0:
>   8     0     0:
>  10     0     0:
>  12     0     0:
>  14     0     0:
>  16     1     1:=
>  18     4     4:==
>  20    12    12:======
>  22    26    26:=============
>  24    21    21:===========
>  26    35    35:==================
>  28    30    30:===============
>  30    10    10:=====
>  32    44    43:======================
>  34     7     6:===-
>  36     4     2:=-
>  38    10     6:===--
>  40     7     3:==--
>  42     4     5:==+
>  44     2     2:=
>  46     1     1:=
>  48     5     6:===
>  50     0     2:+
>  52     0     1:+
>  54     0     1:+
>  56     0     1:+
>  58     0     0:
>  60     0     1:+
>  62     0     1:+
>  64     0     0:
>  66     0     1:+
>  68     0     1:+
>  70     0     0:
>  72     0     1:+
>  74     0     0:
>  76     0     0:
>  78     0     0:
>  80     0     0:
>> 80     0     0:
> mean initn score:  23.8 (2.83)
> mean init1 score:  23.8 (2.83)


>The best scores are:				            init1 initn opt

>Pir1:Cbms  Cytochrome b - Mouse mitochondrion (SGC1)          39    72    40
>Pir1:Cbrt  Cytochrome b - Rat mitochondrion (SGC1)            39    68    40
>Pir1:Cbqfr  Cytochrome b - Rhodospirillum rubrum              39    66    41
>Pir1:Cbhu  Cytochrome b - Human mitochondrion (SGC1)          37    62    39
>Pir1:Wmrz17  Cytochrome b6-f complex 17K protein - Rice c...  44    59    66
>Pir1:O4hud1  Debrisoquine 4-hydroxylase (EC 1.14.14.-) cy...  37    55    37
>Pir1:Cbbo  Cytochrome b - Bovine mitochondrion (SGC1)         39    54    40
>Pir1:Wmlv17  Cytochrome b6-f complex 17K protein - Liverw...  37    52    57
>Pir1:Cbxl  Cytochrome b - African clawed frog mitochondri...  38    49    42
>Pir1:Ccqfct  Cytochrome c' - Rhodocyclus tenuis               35    49    35
>Pir1:Cbboc6  Cytochrome b-c1 complex, 6.4K protein - Bovine   36    48    41
>Pir1:Cblv55  Cytochrome b559 component E - Liverwort (Mar...  47    47    50
>Pir1:Cbobe  Cytochrome b - Evening primrose mitochondrion     47    47    61
>Pir1:Cbnt55  Cytochrome b559 component E - Common tobacco...  47    47    50
>Pir1:Cbrz55  Cytochrome b559 component E - Rice chloroplast   47    47    50
>Pir1:Cbvf  Cytochrome b - Fava bean mitochondrion             47    47    61
>Pir1:Wmnt17  Cytochrome b6-f complex 17K protein - Common...  45    45    67
>Pir1:Ccrfpp  Cytochrome c' - Rhodobacter capsulatus           33    43    33
>Pir1:Cclm  Cytochrome c - Pacific lamprey                     43    43    46
>Pir1:Cbutb  Cytochrome b - Trypanosoma brucei mitochondri...  32    42    42
>Pir1:Rdbyun  Ubiquinol--cytochrome-c reductase (EC 1.10.2...  42    42    42
>Pir1:Wmsp17  Cytochrome b6-f complex 17K protein - Spinac...  41    41    67
>Pir1:Cbyb55  Cytochrome b559 component psbE - Synechocyst...  41    41    48
>Pir1:O4psz  Denitrification system component nirT - Pseud...  41    41    51
>Pir1:Ccps5a  Cytochrome c551 - Pseudomonas aeruginosa         40    40    41
>Pir1:Cbrz  Cytochrome b - Rice mitochondrion                  39    39    41
>Pir1:Cbzm  Cytochrome b - Maize mitochondrion                 39    39    41
>Pir1:Ccps5f  Cytochrome c551 - Pseudomonas fluorescens bi...  38    38    49
>Pir1:Cbbo5  Cytochrome b5 - Bovine                            38    38    52
>Pir1:Ccps5d  Cytochrome c551 - Pseudomonas sp.                38    38    49
>Pir1:Ccbf6  Cytochrome c6 - Yellow-green alga (Bumillerio...  37    37    39
>Pir1:Ccps5s  Cytochrome c551 - Pseudomonas stutzeri (stra...  37    37    52
>Pir1:Ccps5b  Cytochrome c551 precursor - Pseudomonas stut...  37    37    47
>Pir1:Wmkl17  Cytochrome b6-f complex 17K protein - Chlore...  36    36    38
>Pir1:Cctw5t  Cytochrome c552 - Thermus aquaticus              36    36    36
>Pir1:Cblv6  Cytochrome b6 - Liverwort (Marchantia polymor...  34    34    36
>Pir1:Ccck  Cytochrome c - Yeast (Issatchenkia orientalis)     33    33    36
>Pir1:Ccus  Cytochrome c - Smut fungus (Ustilago sphaerogena)  33    33    34
>Pir1:Cceg  Cytochrome c - Euglena gracilis                    33    33    41
>Pir1:Cchq  Cytochrome c - Yeast (Hansenula anomala)           33    33    37

>Aligning...
> CPU time:  0:00:19
> Output File: Ul08.Fasta
>  PARSONS_A    job terminated at  8-DEC-1992 15:13:16.77

>  Accounting information:
>  Buffered I/O count:             457         Peak working set size:   12603
>  Direct I/O count:              1919         Peak page file size:     21702
>  Page faults:                  15293         Mounted volumes:             0
>  Charged CPU time:           0 00:00:29.73   Elapsed time:     0 00:05:30.12
-- 
+----------------------------------+-------------------------------------+
|    Dr. Reinhard Doelz            | RFC     doelz@urz.unibas.ch         |
|      Biocomputing                | DECNET  20579::48130::doelz         |
|Biozentrum der Universitaet       | X25     022846211142036::doelz      |

From owner-software@net.bio.net Mon Jan 04 22:00:00 1993
Path: biosci!agate!ames!haven.umd.edu!uunet!news.univie.ac.at!chx400!urz.unibas.ch!bioftp.unibas.ch!comp.bioz.unibas.ch!doelz
From: doelz@comp.bioz.unibas.ch (Reinhard Doelz)
Newsgroups: bionet.software
Subject: RE: GCG Synchronisation Problem
Message-ID: <1993Jan5.174756.27917@comp.bioz.unibas.ch>
Date: 5 Jan 93 17:47:56 GMT
References: <1993Jan5.145442.6246@gserv1.dl.ac.uk>
Sender: usenet@comp.bioz.unibas.ch (NEWS transaction account)
Reply-To: doelz@urz.unibas.ch
Distribution: bionet
Organization: EMBnet Switzerland [BASEL]
Lines: 38
Nntp-Posting-Host: biox.embnet.unibas.ch

In article <1993Jan5.145442.6246@gserv1.dl.ac.uk>, PARSONS_A@snd01.pcr.co.uk writes:
...
|> message - makes you wonder what we a re paying GCG maintenance for
|> sometimes.
|> 

I wouldn't be that harsh. Guess where the knowledge comes from first - I 
found the GCG guys to be quite responsive, as compared to other software 
companies. I certainly would appreciate to get all maintenance for nothing 
:-) but I am afraid that we would wait very long to get the next version 
this way. You certainly can flame anyone we ought to pay money  but 
the example chosen isn't the prototype suited for blaming. 

By the way: did you get the databases from GCG or did you get them else-
where, and how much did you pay for getting the databases? If you consider 
the current situation of the database vendors, all people expect that 
the data are available for free on FTP, and certainly formatted in 
all available and possible formats. That is apparently true for some 
databases, but you get what you paid for. I don't want to pay more 
either (in particular, the database you had problems with is not the 
cheapest on the block if you purchase its CD-ROM), but the current funding 
model for databases is that the public state money funds the databases 
with really big money, and the users get it for negotiable money. If 
the current growth continues, I am pessimistic enough to predict more 
of these problems as you had in the future. 

Regards 
Reinhard 

-- 
+----------------------------------+-------------------------------------+
|    Dr. Reinhard Doelz            | RFC     doelz@urz.unibas.ch         |
|      Biocomputing                | DECNET  20579::48130::doelz         |
|Biozentrum der Universitaet       | X25     022846211142036::doelz      |
|   Klingelbergstrasse 70          | FAX     x41 61 261- 6760 or 267- 2078     
|     CH 4056 Basel                | TEL     x41 61 267- 2076 or 2247    |   
+------------- bioftp.unibas.ch is the SWISS EMBnet node ----------------+
               -----------------------------------------

From owner-software@net.bio.net Mon Jan 04 22:00:00 1993
Path: biosci!MED.PITT.EDU!bsh
From: bsh@MED.PITT.EDU (Basavaraju Shankarappa)
Newsgroups: bionet.software
Subject: Re: software for silent mutagenesis
Message-ID: <9301052220.AA09065@miranda.med.pitt.edu>
Date: 5 Jan 93 22:20:49 GMT
References: <9212302202.AA15166@net.bio.net>
Sender: daemon@net.bio.net
Distribution: bionet
Lines: 34

> In article <1992Dec30.185104.9506@alw.nih.gov> usdin@helix.nih.gov (Ted Usdin) writes:
> >Weiner and Scheraga (CABIOS, vol 5 pp191-198, 1989) described a Macintosh
> >program (Gene.Design.?) to assist in introducing restriction sites into DNA without changing the amino
> >acid sequence.  Does anyone know where I can get a copy of this, or a similar
> >program that runs on a Macintosh?
> 
> There is a similar program which runs on a PC called OligoMutantMaker.
> It is available by anonymous ftp from:
> Host fly.bio.indiana.edu
> 
>     Location: /molbio/ibmpc
>            FILE -rw-r--r--       8540  May 14 1991  oligo.doc
>            FILE -rw-r--r--     231882  May 14 1991  oligo.uue
> Dan Jacobson
> danj@welchgate.welch.jhu.edu

	I would like to add that we have also developed a PC compatible
program called SILMUT for identifying regions for the introduction of 
restriction enzyme sites by silent mutagenesis.  Although we have used
the same principle as detailed in the above two programs our approach 
was highly simplified.  This programs just tells you what restriction
enzyme/s can be introduced by silent mutagenesis in a specific sequence
of amino or nucleic acid.  This program is most useful for those who are
contemplating introduction of RE sites to generate casette vectors or
for swapping specific fragments of a gene in a construct.  
	The program is also available on fly.bio.indiana.edu in the 
directory molbio/ibmpc as two files: Silmut.xxx.  
	The work has been published in Biotechinques 12: 882-884 (1992).
The program sets include source codes for the program which has been internally
documented.  The programs work in PC and unix machines but if anyone can 
make it work on a Mac, we would welcome that.

Raj Shankarappa
bsh@med.pitt.edu 

From owner-software@net.bio.net Tue Jan 05 22:00:00 1993
Path: biosci!uwm.edu!cs.utexas.edu!usc!wupost!darwin.sura.net!welchgate.welch.jhu.edu!danj
From: danj@welchgate.welch.jhu.edu (Dan Jacobson)
Newsgroups: bionet.software
Subject: Re: Plasmid draw program for IBM
Message-ID: <1993Jan6.151812.23835@welchgate.welch.jhu.edu>
Date: 6 Jan 93 15:18:12 GMT
References: <9301060448.AA14948@net.bio.net>
Distribution: bionet
Organization: Johns Hopkins Univ. Welch Medical Library
Lines: 32

In article <9301060448.AA14948@net.bio.net> preissj@CLVAX1.CL.MSU.EDU ("J Preiss--Seq Anal") writes:
>Plasmid.drw
>
>I have a request to post to the bio software net.  I want a
>program to draw plasmids for IBM/DOS based computers.  I have
>compiled a wish list of what I want in such a program.  If anyone
>knows of any program that comes close to my desires, I would
>appreciate hearing about it.  
>(wish list deleted)

There are a few programs which draw plasmids for IBM/DOS.  I don't
think that they meet all of your specs but you can probably achieve
what you'd like by the combination of  one of these programs and some
utility programs for the PC.

1) Plasmid - can be retrieved by anonymous ftp (or gopher)
from ftp.bio.indiana.edu in the molbio/ibmpc directory.

2) Plasmidg - which can be retrieved by anonymous ftp from
ftp.bchs.uh.edu in the /pub/gene-server/dos directory

3) Easyclon - which can be retrieved by anonymous ftp from
ftp.bchs.uh.edu in the /pub/gene-server/dos directory

These can also be retrieved by anonymous ftp or gopher from
many of the EMBnet nodes.

Best of luck,

Dan Jacobson

danj@welchgate.welch.jhu.edu

From owner-software@net.bio.net Tue Jan 05 22:00:00 1993
Path: biosci!CLVAX1.CL.MSU.EDU!preissj
From: preissj@CLVAX1.CL.MSU.EDU ("J Preiss--Seq Anal")
Newsgroups: bionet.software
Subject: Small scale Auto Sequence entry system
Message-ID: <9301060448.AA14938@net.bio.net>
Date: 6 Jan 93 04:39:00 GMT
Sender: daemon@net.bio.net
Distribution: bionet
Lines: 61

sequence.pgm

     I am looking for a system for automating entry of sequence
data into the computer in our lab.

     We are a midsized (about 12 of us) biochemistry lab who
sequence 2-10 kb per year to verify clones and site directed
mutants.  We use Sanger di-deoxy sequencing with Sequenase and
35-S-dATP on acrylamide gels and X-ray film.  We use an IBM clone
(386-25) and GCG on a VAX for sequence analysis.

     Currently we read sequence films on a light box, record data
on scratch paper, and type it into the computer for analysis. 
This is tedious and error prone.

     The lab PI recently asked me to recommend a system to enter
sequence data into the computer automatically.  He wants to spend
under $1,000- and wants to stick to the traditional autorad
technology that he feels comfortable with.

     I have a vision of a system in mind, but have no idea if
anything like it exists.  I envision a small scanner device to
enter data into the computer and software which would allow you
to see the autorad on screen.  I would like to see a visual
picture of the computers choices for reading the sequence which
could be edited on screen by the user.  Data should be in a
format which could be easily saved as an ASCII file of sequence
or directly shipped down to the vax for GCG to analyze.

     Ideally, I would like some versatility in the system.  If I
had a scanner on hand, I would like to be able to use it to enter
sequence from old publications or faxed data directly from text
on paper.  I would like the option to digitize pictures of
agarose gels into the computer to use graphics programs to add
pointers and text to the gel picture for publication quality
figures. 

     This system would require some hardware and some software,
of which we have neither.  The last system I used was purchased
around 1986 and I no longer have access to it or any of it's
components.  Any suggestions for hardware and/or software for a
system like this would be greatly appreciated.  In leu of my
dream system, I would also appreciate any recommendations from
people using small scale automated sequence data entry systems. 
Any warnings or advice will be greatly appreciated.

     A summary will be posted back to the net if I get anything
interesting and helpful back.  If I get nothing I will try
posting to autoseqs, but I don't subscribe to that group.


          Thanks

          Dr.  Leonard N. Bloksberg
          PreissJ@clvax1.cl.msu.edu
          Dept. of Biochemistry
          Michigan State University

                         "Life is weird"



From owner-software@net.bio.net Tue Jan 05 22:00:00 1993
Path: biosci!CLVAX1.CL.MSU.EDU!preissj
From: preissj@CLVAX1.CL.MSU.EDU ("J Preiss--Seq Anal")
Newsgroups: bionet.software
Subject: Plasmid draw program for IBM
Message-ID: <9301060448.AA14948@net.bio.net>
Date: 6 Jan 93 04:41:00 GMT
Sender: daemon@net.bio.net
Distribution: bionet
Lines: 105

Plasmid.drw

I have a request to post to the bio software net.  I want a
program to draw plasmids for IBM/DOS based computers.  I have
compiled a wish list of what I want in such a program.  If anyone
knows of any program that comes close to my desires, I would
appreciate hearing about it.  Also, if any programmers monitor
this net, I would like to see such a program written.  I have
heard of several programs for the Mac which are supposed to come
close, but none that really does the job.  I am also aware of
Clone, plus Enhance from Scientific & Educational Software for
IBM.  It is a very nice program, from what I've heard, but a
little out of my price range at $650- last I checked.
     I would invite others to comment on my wish list, add to it,
direct me to their favorite program, or tell me that I am a fool
for asking for so much for so little.  With any luck, I will
either find what I'm looking for, or inspire some enterprising
programmer to write it.

Things I want in a plasmid drawing program for IBM computers.

1.   Low cost, around $250-
     Based on informal surveys of my colleagues, my estimates for
how many copies you could sell, worldwide, of a good program are
as follows:          $250-         10,000
                     $500-          1,000
                    $1000-            100    Ok, very informal.

2.   Ability to take in data and output data in formats that are
easily compatible with other systems and programs.
     Most computer users have more than one program loaded on
their hard drive, and we each have our favorite for doing certain
functions.  My favorite programs are those that allow me to do
what that program does best and transfer to another program for
other functions.  An example, I entered my sequence on a
digitizer with Microgene, edited it with MS Word, performed
sequence analysis on GCG, prepared it for publication on Aldus
Pagemaker and prepared slides to present it at meetings with
Powerpoint.

3.   Ability to take input as either sequence (in ASCII files) or
as point info (eg, this site here, that gene there, on a plasmid
this big).

4.   Ability to produce publication quality plasmid and clone
drawings.  Printed graphics must be clear and sharp and
comparable in print quality with those drawn by graphic artists
and published in existing literature.

5.   Ability to accommodate new genetic elements and features.  

     Different scientists want to highlight different things in
their published plasmid diagrams.  Some elements are point sites,
some cover a region.  Some are directional, some not.  Some
elements are embedded within other other features.  Others are
fragments eg, the 5' region of a gene, and may be linked to
another fragment, eg the 3' region of another gene.  Some
features are too detailed to be shown directly in the diagram and
need to be blown up, eg, a multiple cloning site.

6.   Modifiable or editable final picture.  I sometimes want to
modify things to highlight features I am discussing in the text
or minimize things like mundane vector functions.  Can I have the
final say in how the figure looks please?

7.   Many options for shading and fill patterns in black and
white laser prints.  Most of the scientific community publishes
in black and white.  Color options are nice for seminars, but we
need black and white to publish.

8.   Ability to combine several plasmid and clone drawings on a
single page with other graphics.  This may really be part of my
request to have file formats that can be accessed in many
programs.  If I could paste several clone diagrams into a file in
another program and draw arrows and text between to make flow
diagrams showing the history of construction of a plasmid, that
would be perfect.

9.   Identify all known restriction enzyme sites and names.  I
would like the program to identify, and include in the final
diagram, all sites that I ask it to.  An extension of this is to
have it include all unique cutting enzymes, or all enzymes that
cut less than 3 times or more than 10 times, or what ever I
choose that day.  A useful, but not necessary, side function here
would be the ability to provide a list of restriction fragments
resulting from any single or multiple digest and sizes of
fragments in order of size.

10.  Simple cloning functions to allow creation of new plasmids
from pieces of existing figures.  Nothing fancy is required here,
but it should be able to cut at specified restriction enzyme
sites, modify ends with typical enzymes (eg, Klenow, T4-Pol...)
and ligate, while maintaining the correct resultant sequence.


          Thanks

          Dr.  Leonard N. Bloksberg
          PreissJ@clvax1.cl.msu.edu
          Dept. of Biochemistry
          Michigan State University

                         "Life is weird"



From owner-software@net.bio.net Tue Jan 05 22:00:00 1993
Path: biosci!uwm.edu!cs.utexas.edu!qt.cs.utexas.edu!yale.edu!ira.uka.de!rz.uni-karlsruhe.de!stepsun.uni-kl.de!sun.rhrk.uni-kl.de!andrick
From: andrick@rhrk.uni-kl.de (Ulf Andrick [Biologie])
Newsgroups: comp.ai,comp.ai.neural-nets,sci.cognitive,comp.theory.cell-automata,bionet.neuroscience,bionet.molbio.evolution,bionet.software
Subject: Re: Biologically Plausible Dynamic Artificial Neural Networks
Message-ID: <1993Jan6.224107.27144@rhrk.uni-kl.de>
Date: 6 Jan 93 22:41:07 GMT
References: <726213237snz@manor.demon.co.uk>
Organization: University of Kaiserslautern, Germany
Lines: 97
Xref: biosci comp.ai:1398 comp.ai.neural-nets:1290 sci.cognitive:698 comp.theory.cell-automata:206 bionet.neuroscience:876 bionet.molbio.evolution:720 bionet.software:3889
X-Newsreader: Tin 1.1 PL4

paulf@manor.demon.co.uk (Paul Fawcett) writes:
: 
:           Biologically  Plausible  Dynamic Artificial Neural Networks.
:           -----------------------------------------------------------

Biologically Plausible Artificial Neural Network sounds to me
a bit like an oxymoron. I tend to consider any `Artificial Neural 
Networks' as not biologically plausible. 

: 
:           A   *Dynamic   Artificial   Neural   Network*   (DANN)   [1]
:           possesses   processing  elements  that  are  created  and/or
:           annihilated,  either in real time  or  as  some  part  of  a
:           development phase [2].
: 
:           Of    particular    interest    is    the   possibility   of
:           constructing   *biologically    plausible*    DANN's    that
:           models    developmental   neurobiological   strategies   for
:           establishing  and   modifying processing elements and  their
:           connections.
: 
:           Work  with  cellular  automata in modeling cell genesis  and
:           cell pattern  formation  could  be applicable to the  design
:           of  DANN topologies.  Likewise, biological features that are
:           determined by genetic  or  evolutionary  factors  [3]  would
:           also have a  role  to play.
: 

Cellular automata? One might feel reminded of the Game of
Life, where the cells change their state of being alive or
dead according to the states of the neighbouring cells. If
something like that is suggested, I feel somewhat skeptical if
that is of use. I thought that the main issue of neurogenesis
was the formation of synapses. That means, e. g., how do the
neuronal processes find their way to their targets through a
nascent entanglement of cells (not necessarily neurones, but
also glia)? How is synaptic coupling changed in response to some 
stimulus? So, are your `cells' neurones, processes, synapses, or what?

But perhaps you meant a concept of a cellular automaton so general
that one might consider the use of the word as nearly
meaningless.

At least, the point seems to be a little mute to a person with
some half-knowledge about cellular automata and neurogenesis. 

:           Putting  all  this  together  with  a view to constructing a
:           working DANN,  possessing cognitive/behavioral attributes of
:           a biological system is a tall order; the modeling of nervous
:           systems in simple organisms may be the  best  approach  when
:           dealing with a problem of such complexity [4].

There seems to be enough work to be done to simulate `static' Neural 
Networks in simple organisms. An interesting question is, e. g.,
what role the complex electrophysiological properties of the single 
neuron play for the behaviour of the whole network? What are
the effects of neuromodulators? And these questions may
also be of relevance in neural development. 

Artificial Neural Networks do hardly play any role in that
kind of research, IMVHO, unless they have sophisticated
neuronal properties, which most information scientists never
dream of, but I wouldn't call such a model Artificial Network
in order to distinguish it from much more primitive devices,
which might be appropriate to describe spin glasses or
whatever.

As you can see, my view is that the Artificial Neural Network research 
is an engineering discipline detached from natural paradigmata,
just as the whole AI. (As this is also crossposted to AI
groups, I expect to have to put on my flame-proof suit.)

: 
:           Any  comments,  opinions  or  references  in respect of  the
:           above assertions would be most welcome.
: 
: 
:           Many thanks
: 
:           Paul Fawcett.
: 
:           University of Westminster
: 
: 
:           References.
[deleted]

--
Ulf R. Andrick                                andrick@rhrk.uni-kl.de
FB Biologie - Tierphysiologie
Universitaet                             Was du nicht selber weiszt, 
D-W 6750 Kaiserslautern       das muszt du dir erklaeren (Tegtmeier)
-- 
Ulf R. Andrick                                andrick@rhrk.uni-kl.de
FB Biologie - Tierphysiologie
Universitaet                             Was du nicht selber weiszt, 
D-W 6750 Kaiserslautern       das muszt du dir erklaeren (Tegtmeier)

From owner-software@net.bio.net Wed Jan 06 22:00:00 1993
Path: biosci!uwm.edu!caen!kuhub.cc.ukans.edu!husc-news.harvard.edu!husc.harvard.edu!robison1
From: robison1@husc10.harvard.edu (Keith Robison)
Newsgroups: bionet.software
Subject: Experiences with Entrez under UNIX?
Message-ID: <robison1.726426691@husc.harvard.edu>
Date: 7 Jan 93 17:11:31 GMT
Lines: 16
Nntp-Posting-Host: husc10.harvard.edu

Does anyone out there have NCBI's Entrez running under UNIX?
What machine are you using, and how much trouble was it to
install and how reliable is it?  I had heard rumors of it
being a difficult beast to install and maintain, but that
was 6 months ago so maybe if they had any basis they are
now false.

Thanks.


Keith Robison
Harvard University
Department of Cellular & Developmental Biology
Department of Genetics / HHMI

robison@biosun.harvard.edu 

From owner-software@net.bio.net Wed Jan 06 22:00:00 1993
Path: biosci!agate!spool.mu.edu!yale.edu!ira.uka.de!chx400!urz.unibas.ch!bioftp.unibas.ch!comp.bioz.unibas.ch!doelz
From: doelz@comp.bioz.unibas.ch (Reinhard Doelz)
Newsgroups: bionet.molbio.genbank,bionet.software
Subject: This is a question to bionet.software, commercial issue.
Message-ID: <1993Jan7.072233.9124@comp.bioz.unibas.ch>
Date: 7 Jan 93 07:22:33 GMT
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With respect to the discussion going on about Maspar's posting on 
the nicety of the BLAZE program, I would like to ask whether companies 
like GCG woudln't have the same rights? They've been public domain for 
a bit longer than BLAZE, and still each time I inquired about a 
bionet.software.gcg newsgroup I was told by Dake K. that this is not an 
issue (sorry that I don't quote the mail here, the last was already some 
time back and I deleted them.) Despite the full appreciation of the 
beauty and beneficious nature of BIONET I start to worry whether there 
isn't some bias coming in in interpreting the rules. There are hundreds 
of postings in comp.sys which permanently reflect commercial issues. 
So why not have the policy adopted for BIONET also? In USENET terminology, 
BIONET still does behave a little different (just remember voting, email gw 
etc), but wouldn't it be time to revise this fear of being commercial and 
afterwards filter (other would call it censor) at the justice of 
administrators? 

Regards 
Reinhard 


-- 
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|    Dr. Reinhard Doelz            | RFC     doelz@urz.unibas.ch         |
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From owner-software@net.bio.net Wed Jan 06 22:00:00 1993
Path: biosci!agate!spool.mu.edu!uunet!mcsun!uknet!daresbury!news
From: PARSONS_A@snd01.pcr.co.uk
Newsgroups: bionet.software
Subject: Synchronisation Problem in GCG with PIR : Solution & description
Message-ID: <1993Jan7.105853.21680@gserv1.dl.ac.uk>
Date: 7 Jan 93 10:58:17 GMT
Sender: list-admin@daresbury.ac.uk
Distribution: bionet
Lines: 41
Original-To: BIO-SOFT <BIO-SOFT@UK.AC.DARESBURY>

Hi Netfolk,

David Mathog (Caltech) mailed me to post to the net what the solution to my
problem was, as had thanked all those who had helped me.  Reinhard Doelz
(Basel) and Liz Cowe (Oxford) both gave me the answer to fix the problem but Liz
also provided an in depth analysis of the cause - with her agreement I am
posting her analysis and am also alerting MIPS who provided the copy of PIR
in question.

Viva la (le?) Net!

Tony p.


==============================================================================
To: parsons_a@uk.co.pcr.snd01
Message-ID: <009662A5.93B2E8A0.15248@vax.path.ox.ac.uk>
Subject: GCG syncoronization problem

Tony,

	I have also seen this problem with the PIR 