From owner-software@net.bio.net Sat Feb 01 22:00:00 1997
Path: biosci!agate!howland.erols.net!news.sprintlink.net!news-peer.sprintlink.net!news.voicenet.com!news
From: "George A. Heavner" <gheavner@voicenet.com>
Newsgroups: bionet.software
Subject: Re: Short seq search?
Date: Sat, 01 Feb 1997 20:41:39 -0500
Organization: Voicenet - Internet Access - (215)674-9290
Lines: 10
Message-ID: <32F3F0D3.2F14@voicenet.com>
References: <32F371F8.71B1@usfca.edu>
NNTP-Posting-Host: paoli26-pri.voicenet.com
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.0Gold (WinNT; U)

Chihara wrote:
> 
> Is there a program that will search genbank or prosite etc. for short
> amino acid sequnces?  i.e. 5-7 residues?
> Thanks


Try a BLAST search at http://www.ncbi.nlm.nih.gov/BLAST/

   George A. Heavner

From owner-software@net.bio.net Sat Feb 01 22:00:00 1997
Path: biosci!agate!spool.mu.edu!uwm.edu!www.nntp.primenet.com!nntp.primenet.com!news.maxwell.syr.edu!insync!Gamma.RU!srcc!news.rcom.ru!infopro!not-for-mail
From: "Ekaterina E. Mikhailova" <kath@isc.nw.ru>
Newsgroups: bionet.software,bionet.molec-model
Subject: Re: Program for molecular modeling.
Date: 2 Feb 1997 05:17:02 GMT
Organization: WinWare
Lines: 36
Message-ID: <01bc10c8$6cf79600$7601f2c3@any.rcom.spb.su>
References: <Pine.OSF.3.95.970125173803.2874B-100000@saturn.mahidol.ac.th> <32ECCC08.7352@rockvax.rockefeller.edu>
NNTP-Posting-Host: dialup-118.infopro.spb.su
X-Newsreader: Microsoft Internet News 4.70.1155
Xref: biosci bionet.software:17763 bionet.molec-model:1350



Roberto Sanchez <sancher@rockvax.rockefeller.edu> wrote in article
<32ECCC08.7352@rockvax.rockefeller.edu>...
> Panapat Uawithya wrote:
> > 
> > Dear all,
> >  I wold like to know the name of the software other than from MSI and
> > Waht_if. Are there any that can do molecular modeling for protein?
> 
> Have a look at our page for MODELLER at :
> 
> http://guitar.rockefeller.edu/modeller/modeller.html
> 
> Modeller is a protein modeling program mainly used in comparative
> modeling. It is free to academics.
> 
> best wishes,
> 
> Roberto

Hi!

Dear Panapat! 

Probably, the program that you are looking for is on the
http://www.scivision.com 
It is called SciProtein for Alchemy 2000

Alexei Leznikov
alex@isc.nw.ru

Winware Unlimited
St. Petersburg
RUSSIA


From owner-software@net.bio.net Sat Feb 01 22:00:00 1997
Path: biosci!agate!spool.mu.edu!howland.erols.net!usenet.kornet.nm.kr!ames!purdue!oitnews.harvard.edu!news
From: Robin Colgrove <robin@louis.bidmc.harvard.edu>
Newsgroups: bionet.software
Subject: Re: Short seq search?
Date: Sat, 01 Feb 1997 23:02:29 -0500
Organization: Beth Israel Hospital, Harvard Medical School
Lines: 7
Message-ID: <32F411CC.543E@louis.bidmc.harvard.edu>
References: <32F371F8.71B1@usfca.edu>
Reply-To: robin@louis.bidmc.harvard.edu
NNTP-Posting-Host: louis.bidmc.harvard.edu
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.01 (Macintosh; I; PPC)

I wrote such a program back in the mid 80's
before the "blast" era. It's in C.
I haven't thought mouch about it since there are
so many net tools around these days but if it
would be useful, I can dig it our and post it.

robin colgrove

From owner-software@net.bio.net Sat Feb 01 22:00:00 1997
Path: biosci!agate!howland.erols.net!vixen.cso.uiuc.edu!uwm.edu!newsspool.doit.wisc.edu!news.doit.wisc.edu!ebv.oncology.wisc.edu!aiyar
From: aiyar@ebv.oncology.wisc.edu (Ashok Aiyar)
Newsgroups: bionet.software
Subject: Software/code to search for palindromes?
Date: 2 Feb 1997 01:53:34 GMT
Organization: Sugden Lab, McArdle Laboratory for Cancer Research, UW-Madison
Lines: 21
Message-ID: <slrn5f7ssu.o85.aiyar@ebv.oncology.wisc.edu>
Reply-To: aiyar@ebv.oncology.wisc.edu
NNTP-Posting-Host: ebv.oncology.wisc.edu
X-Newsreader: slrn (0.9.2.1 BETA UNIX)


I am looking for C/C++ source, or a compiled binary that will
let me search through sequences for perfect or imperfect
palindromes.

If only a binary is available, it must run under one of the 
following OSes:
IRIX
DOS
Windows
MacOS
Linux
Digital Unix

Thank you,
Ashok

-- 
Ashok Aiyar, Ph.D.
McArdle Laboratory for Cancer Research
aiyar@ebv.oncology.wisc.edu

From owner-software@net.bio.net Sat Feb 01 22:00:00 1997
Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!newsfeeds.sol.net!hunter.premier.net!www.nntp.primenet.com!nntp.primenet.com!news.bbnplanet.com!su-news-hub1.bbnplanet.com!su-news-feed4.bbnplanet.com!jeeves.usfca.edu!news
From: Chihara <chihara@usfca.edu>
Newsgroups: bionet.software
Subject: Short seq search?
Date: Sat, 01 Feb 1997 16:40:24 +0000
Organization: University of San Francisco
Lines: 3
Message-ID: <32F371F8.71B1@usfca.edu>
Reply-To: chihara@usfca.edu
NNTP-Posting-Host: annex41.usfca.edu
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 2.02 (Macintosh; I; 68K)

Is there a program that will search genbank or prosite etc. for short 
amino acid sequnces?  i.e. 5-7 residues? 
Thanks

From owner-software@net.bio.net Sun Feb 02 22:00:00 1997
Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!howland.erols.net!news.nacamar.de!uni-erlangen.de!winx03!wpxx02!not-for-mail
From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel)
Newsgroups: bionet.software
Subject: Re: Short seq search?
Date: 3 Feb 1997 00:47:07 GMT
Organization: University of Wuerzburg, Germany
Lines: 17
Message-ID: <5d3cib$7ep@winx03.informatik.uni-wuerzburg.de>
References: <32F371F8.71B1@usfca.edu> <32F3F0D3.2F14@voicenet.com>
NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de
X-Newsreader: TIN [UNIX 1.3 950824BETA PL0]

George A. Heavner (gheavner@voicenet.com) wrote:
> Chihara wrote:
> > Is there a program that will search genbank or prosite etc. for short
> > amino acid sequnces?  i.e. 5-7 residues?
> 
> Try a BLAST search at http://www.ncbi.nlm.nih.gov/BLAST/

BLAST usually doesn't work with short query sequences because the
alignment produced is not significant. However, FASTA searches work
fine.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004@rzbox.uni-wuerzburg.de  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */

From owner-software@net.bio.net Sun Feb 02 22:00:00 1997
Path: biosci!daresbury!yama.mcc.ac.uk!hydraulix.bangor.ac.uk!thunder!bss194
From: bss194@thunder (K.James)
Newsgroups: bionet.software
Subject: Re: Oligonucleotide databases
Date: 3 Feb 1997 11:03:30 GMT
Organization: University of Wales, Bangor.
Lines: 29
Message-ID: <5d4gm2$l8n@hydraulix.bangor.ac.uk>
References: <32F21D41.66AB@anl.gov>
NNTP-Posting-Host: thunder.bangor.ac.uk
X-Newsreader: TIN [version 1.2 PL2]

James Akowski (akowski@anl.gov) wrote:
: Hello,

: 	Does anyone know of software for cataloging/organizing
: oligonucleotides?  I want to start a database before the number of
: oligos in our lab gets too large.  We have Excel and QuatroPro but I do
: not wish to spend time learning these - I would appreciate any pointers
: to existing templates/front-ends for these programs or any other PC or
: Unix database software for oligonucleotides.

Being in the same position a year ago I wrote some VBA code in Excel to do
this. You are welcome to a copy if you want it. Its main limitations are
that it stores the details as a flat file within the spreadsheet, no
relational database this one! Also it has no special printout option to 
dump a search result to the printer.

It stores name, sequence, owner, design method, date etc. and maintains 
the list's integrity by indexing on the oligo name i.e. when you enter a 
new oligo it won't let you give it the same name as an existing one. It 
will also ensure certain information is entered. Data is entered using a 
dialog with dropdowns for designer, supplier, date and so on. Searching 
uses Excel's own search dialogs.

--
Keith James Ph.D. - k.james@bangor.ac.uk  PGP 2.6.2i  Key ID 469A9FA1
Biodegradation Group                         *Encrypt and Survive*  
School of Biological Sciences             Nightmare: Quake me up now!
University of Wales, Bangor, UK                                     
-------http://oracle.bangor.ac.uk/sbs/research/biodegradation/-------

From owner-software@net.bio.net Sun Feb 02 22:00:00 1997
Path: biosci!daresbury!nntp-trd.UNINETT.no!sn.no!hermod.uio.no!nntp.uio.no!newsfeeds.sol.net!newspump.sol.net!howland.erols.net!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!uunet!in3.uu.net!137.208.127.3!newsfeed.wu-wien.ac.at!usenet
From: bunka@i112pc09.vu-wien.ac.at (Sebastian Bunka)
Newsgroups: bionet.software
Subject: Re: Looking for BOB
Date: 3 Feb 1997 09:52:12 GMT
Organization: Vet. University Vienna
Lines: 23
Message-ID: <5d4cgc$id@cantine.wu-wien.ac.at>
References: <m.mitchell-ya02408000R3101971520150001@news.lif.icnet.uk>
NNTP-Posting-Host: i112pc09.vu-wien.ac.at
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
X-Newsreader: knews 0.9.8

In article <m.mitchell-ya02408000R3101971520150001@news.lif.icnet.uk>,
	m.mitchell@icrf.icnet.uk (Mike Mitchell) writes:
> I am trying to find a source for BOB, the Blast Output Browser. Can any one
> help?
> 
You can check for the source of tbob, a blast output parser:

Host bioinformatics.weizmann.ac.il

    Location: /pub/software/unix
           FILE -r--r--r--      25842  May 30 1994  tbob.tar.gz

SWB

-- 

;-)
------------------------------------------------------------------------
Sebastian Bunka                 ph. (+43-1) 250 77 4208
Institute of                    FAX (+43-1) 250 77 4290
Medical Chemistry               email: Sebastian.Bunka@vu-wien.ac.at
University of Veterinary Medicine - Vienna - Austria
------------------------------------------------------------------------

From owner-software@net.bio.net Sun Feb 02 22:00:00 1997
Newsgroups: bionet.software
Path: biosci!agate!spool.mu.edu!howland.erols.net!news.sprintlink.net!news-peer.sprintlink.net!rill.news.pipex.net!pipex!uknet!usenet1.news.uk.psi.net!uknet!uknet!newsfeed.ed.ac.uk!leeds.ac.uk!news
From: bmb5meb@bmb.leeds.ac.uk (M E Beck)
Subject: Re: MS Word to HTML converter
Message-ID: <32F5EB0E.41C6@bmb.leeds.ac.uk>
NNTP-Posting-Host: bmbsgi11.leeds.ac.uk
X-Mailer: Mozilla 3.0Gold (X11; I; IRIX 5.3 IP22)
Content-Type: text/plain; charset=us-ascii
Organization: University of Leeds
MIME-Version: 1.0
Date: Mon, 3 Feb 1997 13:41:42 +0000 (GMT)
References: <32F0B4DD.41C6@bmb.leeds.ac.uk>
    <32f3edce.6348911@news.uhura.minet.net>
Lines: 32
Content-Transfer-Encoding: 7bit

Thanks for everyone's help on this. I've tried Internet Assistant and it
does exactly what I want, saving me from a lot of HTML coding!  :)

Mike.

Russell Holliman wrote:
> 
> Have you tried Microsoft's Internet Assistant for Word? Have not tried
> it myself, but it is supposed to be a (free) add-in for word that lets
> you save the document directly to an HTML file from within word.
> 
> You can download it from their web site at:
> http://www.microsoft.com/word/internet/ia/
> 
> Hope this helps.
> 
> Russell Holliman
> 
> On Thu, 30 Jan 1997 14:50:18 +0000 (GMT), bmb5meb@bmb.leeds.ac.uk (M E
> Beck) wrote:
> 
> >Hi there,
> >
> >I'm trying to create some online documentation which is currently in
> >MS Word format. Does anyone know of any freely available conversion
> >program which will convert from this format to HTML, keeping as much of
> >the formatting (bold type, italics, etc.) as possible?
> >
> >All help appreciated,
> >
> >Mike.
> >

From owner-software@net.bio.net Sun Feb 02 22:00:00 1997
Path: biosci!daresbury!not-for-mail
From: Richard Cooke <richard@sunlpv1.univ-perp.fr>
Newsgroups: bionet.software
Subject: Re: Looking for BOB
Date: 3 Feb 1997 14:51:24 -0000
Lines: 30
Sender: lpddist@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <5d4u1c$4m2@mserv1.dl.ac.uk>
X-Sender: cooke@gala.univ-perp.fr
Original-To: bio-software@dl.ac.uk

In article <m.mitchell-ya02408000R3101971520150001@news.lif.icnet.uk>,
m.mitchell@icrf.icnet.uk (Mike Mitchell) wrote:

 >  I am trying to find a source for BOB, the Blast Output Browser. Can any one
 >  help?
 >

There used to be a graphical interface called BOB at
ftp://milo.dcrt.nih.gov/pub. The source is available as well as executables
for a fair number of platforms but I don't know if it has been updated to
read more recent BLAST output.

Andy (big nose) Law replied:

>I've written a Macintosh version.

Is that a graphical Mac version, Andy? Is it available?

Richard Cooke
(medium-sized nose in Edinburgh some time ago)

Richard Cooke
LPBMP UMR5545 CNRS
UNIVERSITE DE PERPIGNAN
FRANCE
Tel: +33 (0)468662131
Fax: +33 (0)468668499
cooke@univ-perp.fr



From owner-software@net.bio.net Sun Feb 02 22:00:00 1997
Path: biosci!angis.su.OZ.AU!tim
From: tim@angis.su.OZ.AU (Tim Littlejohn)
Newsgroups: bionet.software
Subject: Re: Looking for BOB
Date: 3 Feb 1997 02:30:32 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 17
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <Pine.GSO.3.95.970203212943.12081G-100000@morgan.angis.su.OZ.AU>
References: <5d4cgc$id@cantine.wu-wien.ac.at>
NNTP-Posting-Host: net.bio.net


The original distribution site for tbob is from the Canadian Organelle
Genome Program's site at:

    http://megasun.bch.umontreal.ca/abouttb.html

Cheers,

Tim Littlejohn
 

--------------------------------------------------------------------------
|   ANGIS- The Australian National Genomic Information Service           |
|    http://www.angis.su.oz.au      email: tim@angis.su.oz.au            |
|     Phone Toll Free: 1 800 728 028     FAX: 61-2-9351 2948             |
--------------------------------------------------------------------------


From owner-software@net.bio.net Sun Feb 02 22:00:00 1997
Path: biosci!daresbury!is.bbsrc.ac.uk!Andy.Law
From: Andy.Law@bbsrc.ac.uk (Andy Law)
Newsgroups: bionet.software
Subject: Re: Looking for BOB
Date: Mon, 03 Feb 1997 09:47:11 +0000
Organization: Roslin Institute
Lines: 18
Message-ID: <Andy.Law-ya023480000302970947110001@is.bbsrc.ac.uk>
References: <m.mitchell-ya02408000R3101971520150001@news.lif.icnet.uk>
NNTP-Posting-Host: pc0734.ri.bbsrc.ac.uk
Mime-Version: 1.0
Content-Type: text/plain; charset=ISO-8859-1
Content-Transfer-Encoding: 8bit
X-Newsreader: Yet Another NewsWatcher 2.3.4

In article <m.mitchell-ya02408000R3101971520150001@news.lif.icnet.uk>,
m.mitchell@icrf.icnet.uk (Mike Mitchell) wrote:

 >  I am trying to find a source for BOB, the Blast Output Browser. Can any one
 >  help?
 >  

I've written a Macintosh version.

I think you can get tbob at ftp.ebi.ac.uk (/pub/software/unix ??). I'm not
sure if BOB was there though.

Later,

Andy Law
------------------
( Andy.Law@bbsrc.ac.uk )
( Big Nose in Edinburgh )

From owner-software@net.bio.net Sun Feb 02 22:00:00 1997
Path: biosci!daresbury!nntp-trd.UNINETT.no!online.no!sn.no!www.nntp.primenet.com!nntp.primenet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!uunet!in3.uu.net!198.168.54.138!rcogate.rco.qc.ca!altitude!usenet
From: Xuefeng Yu <xuefeng@ibex.ca>
Newsgroups: bionet.software
Subject: experimental design software
Date: Mon, 03 Feb 1997 10:41:05 -0500
Organization: Communications Accessibles Montreal, Quebec Canada
Lines: 3
Message-ID: <32F60710.74C1@ibex.ca>
NNTP-Posting-Host: ibex.hip.cam.org
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.01 (Macintosh; I; 68K)

I'm looking for statistical experimental design software for Mac or
Windows. If anybody know where I can find a good one please let me know. 
My email address is yuxu@ibex.ca

From owner-software@net.bio.net Sun Feb 02 22:00:00 1997
Path: biosci!daresbury!lyra.csx.cam.ac.uk!news.ox.ac.uk!ermine.ox.ac.uk!enriquez
From: Pita Enriquez Harris <enriquez@ermine.ox.ac.uk>
Newsgroups: bionet.software
Subject: Plasmid drawing software for PC??
Date: Mon, 3 Feb 1997 14:07:01 +0000
Organization: Oxford University
Lines: 18
Message-ID: <Pine.OSF.3.91.970203140529.30711C-100000@ermine.ox.ac.uk>
NNTP-Posting-Host: ermine.ox.ac.uk
Mime-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII



Does anyone know of a good program for drawing/managing DNA constructs on 
a PC?

If you know of where I can get a free download of a demo, that would be 
especially helpful!

Thanks,
Pita

******************************************************************************
Dr. Pita Enriquez Harris           "Waste no more time arguing what a     
Nuffield Department of Medicine           good man should be.  Be one."
Oxford Radcliffe Hospital                                  Marcus Aurelius 
http://users.ox.ac.uk/~enriquez             
******************************************************************************


From owner-software@net.bio.net Mon Feb 03 22:00:00 1997
Path: biosci!agate!spool.mu.edu!uwm.edu!www.nntp.primenet.com!nntp.primenet.com!cs.utexas.edu!news.unt.edu!news
From: "C. J. Fields" <cfields@gab.unt.edu>
Newsgroups: bionet.software
Subject: Re: Plasmid drawing software for PC??
Date: Tue, 04 Feb 1997 00:12:05 -0600
Organization: University of North Texas	
Lines: 36
Message-ID: <32F6D335.69D3@gab.unt.edu>
References: <Pine.OSF.3.91.970203140529.30711C-100000@ermine.ox.ac.uk>
Reply-To: cfields@gab.unt.edu
NNTP-Posting-Host: den1-2-24.dialup.unt.edu
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.0Gold (Win95; I)

Pita Enriquez Harris wrote:
> 
> Does anyone know of a good program for drawing/managing DNA constructs on
> a PC?
> 
> If you know of where I can get a free download of a demo, that would be
> especially helpful!
> 
> Thanks,
> Pita

Try Don Gilbert's excellent IUBIO archive at:

gopher://fly.bio.indiana.edu:70/11/IUBio-Software%2bData

It should be under molecular biology in ibmpc.  VNTI has a great demo,
and I think a few others may be floating around (Plasmid Toolkit is a
more affordable one).

I believe there are ftp and html links as well, if you don't like
gophers.
-- 
C. J. Fields
Graduate Student, Dept. of Biological Sciences
The University of North Texas
Denton, TX 

email : cfields@gab.unt.edu
\\\\\\\\\\\\\\\\\\\\oooooooooooooooo////////////////////
"Giving money and power to government is like giving
 whiskey and car keys to teenage boys"
				-P. J. O'Rourke
"Join the military.  Travel to exotic places, meet 
exciting people, then kill them"
				-Anonymous
////////////////////oooooooooooooooo\\\\\\\\\\\\\\\\\\\\

From owner-software@net.bio.net Mon Feb 03 22:00:00 1997
Path: biosci!agate!howland.erols.net!surfnet.nl!news.unisource.nl!xs4all!not-for-mail
From: marker@xs4all.nl (Niels Groot)
Newsgroups: bionet.software
Subject: Re: Plasmid drawing software for PC??
Date: 4 Feb 1997 06:35:10 GMT
Organization: XS4ALL, networking for the masses
Lines: 30
Message-ID: <5d6lau$cuo$1@news1.xs4all.nl>
References: <Pine.OSF.3.91.970203140529.30711C-100000@ermine.ox.ac.uk>
NNTP-Posting-Host: news.xs4all.nl
Mime-Version: 1.0
Content-Type: Text/Plain; charset=US-ASCII
X-XS4ALL-Date: Tue, 04 Feb 1997 07:35:10 CET
X-Newsreader: WinVN 0.99.7

In article <Pine.OSF.3.91.970203140529.30711C-100000@ermine.ox.ac.uk>, 
enriquez@ermine.ox.ac.uk says...
>
>
>
>Does anyone know of a good program for drawing/managing DNA constructs on 
>a PC?
>
>If you know of where I can get a free download of a demo, that would be 
>especially helpful!
>
>Thanks,
>Pita
 you could take a look at SeqSearch v1.1 at http://www.xs4all.nl/~marker

-multi user
-multi sequence database including contructs
-restriction site analyis
-mismatch primer design
-clone bench
-silent mutagenesis analysis
-mutagenic primer design
-hydrophobicity plots
-DNA consensus search

regards

Niels
marker@xs4all.nl


From owner-software@net.bio.net Mon Feb 03 22:00:00 1997
Path: biosci!daresbury!nntp-trd.UNINETT.no!Oslo2.Norway.EU.net!Norway.EU.net!nntp.uio.no!news.maxwell.syr.edu!news.bc.net!unixg.ubc.ca!info.ucla.edu!ihnp4.ucsd.edu!news.service.uci.edu!mangalam
From: mangalam@uci.edu (Harry Mangalam)
Newsgroups: bionet.software,bionet.molbio.methds-reagnts
Subject: Re: Restriction Enzymes in DNA Strider?
Date: Tue, 04 Feb 1997 14:52:21 -0800
Organization: Dev+Cell Biology, UC Irvine
Lines: 53
Message-ID: <mangalam-ya02408000R0402971452210001@128.200.15.230>
References: <nc1-0402971130080001@phanes94.mc.duke.edu>
NNTP-Posting-Host: skink.bio.uci.edu
Mime-Version: 1.0
Content-Type: text/plain; charset=ISO-8859-1
Content-Transfer-Encoding: 8bit
X-Newsreader: Yet Another NewsWatcher 2.4.0
Xref: biosci bionet.software:17786 bionet.molbio.methds-reagnts:54367

In article <nc1-0402971130080001@phanes94.mc.duke.edu>, nc1@acpub.duke.edu
(Namjin Chung) wrote:
> I use the above software for some sequence processing, and I think it's
> simple but very good software.  The only one thing I'm not satisfied with
> it is that it would not allow custom restriction by a selected set of
> REs.  While MacVector allows you to cut DNA with the enzyme set you
> currently have, it is big and key-locked.
> 
> Does anybody know how to solve this problem?  TIA.

You can solve this with DNA Strider by making subfiles of the enzymes that
you want and renaming them "RELibrary" as you need them, although you'll
have to restart Strider evey time you want to use another subset.  Not a
very elegant way of doing it, but it allows you to keep using the program
you're used to.

Most commercial sequence analysis apps allow you to specify subsets of
enzymes by explicit groupings, or other characteristics, such as all 5'
cutters, those that cut more or less than X times, those that recognize
only sequences more than Y bases, etc.

I've put a web interface on my *FREE* restriction enzyme analysis app
'tacg', and you can specify enzymes via overhang, magnitude of recognition
sequence, number of cuts, explicit picks, subsets via different files (ie
enzymes from different vendors, or sorted via price), etc, etc, etc. Also
works with the transcription factor database, as a quick and dirty version
of Dan Prestridge's 'signalscan'.

Output is via linear map, 1/3/6 frames of translation in 1 or 3 letter
code, pseudo-graphical ladder map and/or gel simulation, fragments (sorted
or not), cut site, etc.  It handles sequence into the millions of bases, if
you have the RAM; it was designed for scanning genome sized chunks of DNA.

   The idea was to get Strider-like output for unix systems via a
command-line program.  It just happened that it's relatively easy to port
it to the web, so I did.

YOu can get a better idea about it by peeking at the web page:

http://hornet.bio.uci.edu/~hjm/projects/tacg/tacg.main.html

the Web form is at:

http://hornet.bio.uci.edu/~hjm/projects/tacg/tacg.main.html

If you use Netscape, you can upload files to it as well as using paste-in;
I'm not sure about other browsers.

Cheers
Harry
-- 
Harry J Mangalam, MolBio+Biochem / Dev+Cell Bio, Rm 4201, BioSciII  UC
Irvine, Irvine, CA, 92717, (714) 824-4824, fax (714) 824 8598

From owner-software@net.bio.net Mon Feb 03 22:00:00 1997
Path: biosci!daresbury!is.bbsrc.ac.uk!Andy.Law
From: Andy.Law@bbsrc.ac.uk (Andy Law)
Newsgroups: bionet.software
Subject: Re: Looking for BOB
Date: Tue, 04 Feb 1997 09:39:12 +0000
Organization: Roslin Institute
Lines: 40
Distribution: bionet
Message-ID: <Andy.Law-ya023480000402970939120001@is.bbsrc.ac.uk>
References: <5d4u1c$4m2@mserv1.dl.ac.uk>
NNTP-Posting-Host: pc0734.ri.bbsrc.ac.uk
Mime-Version: 1.0
Content-Type: text/plain; charset=ISO-8859-1
Content-Transfer-Encoding: 8bit
X-Newsreader: Yet Another NewsWatcher 2.3.4

 >  
 >  Andy (big nose) Law replied:
 >  
 >  >I've written a Macintosh version.
 >  
 >  Is that a graphical Mac version, Andy? Is it available?
 >  

Yes, it is a graphical version. It puts the list of high scores in the top
pane of a window and as you select different hits in that list, it displays
the corresponding alignment in the lower pane. Its not quite finished (I'm
working on an extra bit to allow you to pull the hit sequence via a Web
browser link to an SRS server) and I have to go through a complex
"permission to publish" procedure, but hopefully in the next couple of
months.

When you tie this together with Internet Config and another program that I
wrote to filter the results automatically, you get quite an elegant system
for those who prefer to use a Mac as their main machine i.e.
    

                      Run Blasts on Unix Machine
                                  |
                         Download to Mac by FTP
                                  |
                     Internet Config hands off files
                     ending '.blast' to Blast Filter
                                  |
                Blast Filter checks for significant hits
                                  |
                   +--------------+--------------+
                   |                             |
            Significant Hits               No Significant hits
                   |
                 MacBOB

Andy Law
------------------
( Andy.Law@bbsrc.ac.uk )
( Big Nose in Edinburgh )

From owner-software@net.bio.net Mon Feb 03 22:00:00 1997
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!www.nntp.primenet.com!nntp.primenet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!howland.erols.net!ix.netcom.com!netcom.net.uk!amd-5x86-166mhz
From: berrall@netcomuk.co.uk (Nick Berrall)
Newsgroups: bionet.software
Subject: Visit The Windows Goodies Website
Date: Tue, 04 Feb 97 19:06:30 GMT
Organization: NETCOM Internet Ltd.
Lines: 9
Message-ID: <5d81b6$mfb$3@taliesin.netcom.net.uk>
NNTP-Posting-Host: dialup-07-33.netcomuk.co.uk
X-Newsreader: News Xpress 2.0

The Windows Goodies Website

Please Visit The Windows Goodies Website for software, wallpaper images and
wave files at

http://www.geocities.com/SiliconValley/Pines/7030/index.html

You can also pick up free Java scripts for your Homepages.


From owner-software@net.bio.net Mon Feb 03 22:00:00 1997
Newsgroups: bionet.software
Path: biosci!rutgers!gatech!csulb.edu!hammer.uoregon.edu!arclight.uoregon.edu!feed1.news.erols.com!howland.erols.net!vixen.cso.uiuc.edu!uchinews!ellis.uchicago.edu!cjenkins
From: Cheryl Jenkins <cjenkins@midway.uchicago.edu>
Subject: Need Help Learning Access
X-Sender: cjenkins@ellis.uchicago.edu
X-Nntp-Posting-Host: ellis.uchicago.edu
Content-Type: TEXT/PLAIN; charset=US-ASCII
Message-ID: <Pine.GSO.3.95.970204124930.11032A-100000@ellis.uchicago.edu>
Sender: news@midway.uchicago.edu (News Administrator)
Reply-To: Cheryl Jenkins <cjenkins@midway.uchicago.edu>
Organization: University of Chicago -- Academic Computing Services
Mime-Version: 1.0
Date: Tue, 4 Feb 1997 18:51:40 GMT
Lines: 7

I just took an Access Class and I'm having problems.  Is there a self-help
book out there like "Access for Dummies" or something I can use as a
learning tool at my own pace?

Thanx.



From owner-software@net.bio.net Mon Feb 03 22:00:00 1997
Path: biosci!BTBSOFTWARE.COM!bactbob
From: bactbob@BTBSOFTWARE.COM (Bob Freeman)
Newsgroups: bionet.software
Subject: Free Chlamydia Software, expires 1-98
Date: 3 Feb 1997 22:01:50 -0800
Organization: BtB Software
Lines: 11
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <32F6D0BB.1327@btbsoftware.com>
NNTP-Posting-Host: net.bio.net

Chlamydia software for the Abbott LCx and related systems:
Includes Billing, searching, worksheets, rapid entry, lab slips, 
statistics.  For more information,please see our web site and click on 
"free Chlamydia Software". The software will expire 1-1-98
-- 
Bob Freeman
BtB Software
PO Box 2215
El Centro, CA 92244-2215
Phone/Fax:  619-353-6623
http://www.btbsoftware.com/bactbob

From owner-software@net.bio.net Mon Feb 03 22:00:00 1997
Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!howland.erols.net!quagga.ru.ac.za!ru.uni.net.za!uct.uni.net.za!uct.ac.za!news
From: Ed Rybicki <ed@molbiol.uct.ac.za>
Newsgroups: bionet.software
Subject: Re: Windows: sequence analysis
Date: Tue, 04 Feb 1997 15:57:00 -0400
Organization: Microbiology Dept, Univ Cape Town
Lines: 26
Message-ID: <32F7948C.79E@molbiol.uct.ac.za>
References: <5cpibd$jnl$1@mark.ucdavis.edu>
Reply-To: ed@molbiol.uct.ac.za
NNTP-Posting-Host: 137.158.64.94
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.01 (Win16; I)
To: Zhaomin Yang <ez007215@bullwinkle.ucdavis.edu>

Zhaomin Yang wrote:
> 
> Hi, all,
> 
> Wondering if someone can recommend a good sequence analysis program(s) for
> Windows. I used to use Strider, but the lab I'm in now doesn't have Macs.
> It would be great if the program can draw plasmid maps and can be used for
> designing primers.
> 
> Zhaomin

Hi - we have recently started to use DNAMAN from Lynnon Biosoft up in
Canada; it is remarkably full-featured (if a bit idiosyncratic), and
will do multiple sequence alignment, translation, dna frag assembly, PCR
primer and oligo design...it is also CHEAP ($260 or so).  Works well
under Win 3.1 and is LIGHTNING fast on even long sequence multiple
alignments.  Also does shading of alignments (another perennial q).
-- 
                     Ed Rybicki, PhD  
      Dept Microbiology     |   ed@molbiol.uct.ac.za   
   University of Cape Town  | rybicki@uctvms.uct.ac.za
   Private Bag, Rondebosch  |  phone: x27-21-650-3265
      7700, South Africa    |   fax: x27-21-689 7573
    WWW URL: http://www.uct.ac.za/microbiology/ed.html      
                                        
    "Out here on the perimeter, there are no stars..."

From owner-software@net.bio.net Mon Feb 03 22:00:00 1997
Newsgroups: bionet.software
Path: biosci!agate!howland.erols.net!rill.news.pipex.net!pipex!warwick!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!ebi.ac.uk!news
From: Heikki Lehvaslaiho <Heikki@ebi.ac.uk>
Subject: Re: Looking for BOB
Sender: news@ebi.ac.uk (usenet news)
Message-ID: <32F5BEEB.41C6@ebi.ac.uk>
Date: Mon, 3 Feb 1997 10:33:15 GMT
Content-Transfer-Encoding: 7bit
Content-Type: text/plain; charset=us-ascii
References: <m.mitchell-ya02408000R3101971520150001@news.lif.icnet.uk>
Mime-Version: 1.0
X-Mailer: Mozilla 3.0 (X11; I; IRIX64 6.2 IP19)
Organization: EBI
Lines: 22

Mike Mitchell wrote:
> 
> I am trying to find a source for BOB, the Blast Output Browser. Can any one
> help?

TBOB is one of the products of OGMP - Organelle Genome Megasequencing
Program (http://megasun.bch.umontreal.ca/ogmpproj.html) in Canada.

You'll find the TBOB source and documentation from:

gopher://megasun.bch.umontreal.ca/11/CMB/Databases/Blast/tbob/
 
	-Heikki

______ _/      _/____________________________________________________
      _/      _/
     _/  _/  _/  Heikki Lehvaslaiho               <heikki@ebi.ac.uk>
    _/_/_/_/_/  EMBL Outstation, European Bioinformatics Institute
   _/  _/  _/  Wellcome Trust Genome Campus, Hinxton
  _/  _/  _/  Cambs. CB10 1SD, United Kingdom
     _/      Phone: +44 (0)1223 494 644   FAX: +44 (0)1223 494 468
___ _/_/_/_/_/________________________________________________________

From owner-software@net.bio.net Mon Feb 03 22:00:00 1997
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!status.gen.nz!news.express.co.nz!actrix.gen.nz!usenet
From: lrollo@actrix.gen.nz (Lindsay Rollo)
Newsgroups: bionet.software
Subject: OCR for Scientific/Engineering Material
Date: Tue, 04 Feb 1997 20:24:48 GMT
Organization: Actrix Networks Limited
Lines: 19
Message-ID: <33019af7.9747175@news.actrix.gen.nz>
NNTP-Posting-Host: lrollo.actrix.gen.nz
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Newsreader: Forte Agent .99g/32.339

	I am trying to identify OCR software that specifically caters for
scientific, engineering and other technical special requirements. 

	In particular I'm interested in software that:

	[1]	allows bulk additions to the program's dictionary to allow the
		rapid inclusion of specialist vocabularies;

	[2}	provides drop down boxes of user-selected special characters to permit
		immediate entry of both special symbols and characters commonly used
		in scientific and technical disciplines, eg, the superscript symbol to
		denote degrees of temperature.

	If anyone has experience of this type of data or information capture I'd be
very pleased to receive e-mails.

Lindsay J Rollo                                   lrollo@actrix.gen.nz
Wellington, New Zealand


From owner-software@net.bio.net Mon Feb 03 22:00:00 1997
Path: biosci!rutgers!gatech!csulb.edu!hammer.uoregon.edu!arclight.uoregon.edu!su-news-hub1.bbnplanet.com!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!howland.erols.net!cs.utexas.edu!newshost.convex.com!newsgate.duke.edu!phanes94.mc.duke.edu!user
From: nc1@acpub.duke.edu (Namjin Chung)
Newsgroups: bionet.software,bionet.molbio.methds-reagnts
Subject: Restriction Enzymes in DNA Strider?
Date: 4 Feb 1997 16:28:10 GMT
Organization: Duke University Medical Center
Lines: 15
Message-ID: <nc1-0402971130080001@phanes94.mc.duke.edu>
NNTP-Posting-Host: phanes94.mc.duke.edu
Xref: biosci bionet.software:17782 bionet.molbio.methds-reagnts:54352

Dear DNA Strider Users:

I use the above software for some sequence processing, and I think it's
simple but very good software.  The only one thing I'm not satisfied with
it is that it would not allow custom restriction by a selected set of
REs.  While MacVector allows you to cut DNA with the enzyme set you
currently have, it is big and key-locked.

Does anybody know how to solve this problem?  TIA.


Namjin Chung, who knows little and asks a lot!
Program in Molecular Cancer Biology
Duke University Medical Center
Durham, North Carolina

From owner-software@net.bio.net Mon Feb 03 22:00:00 1997
Newsgroups: bionet.software
Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!Norway.EU.net!EU.net!uknet!usenet1.news.uk.psi.net!uknet!uknet!strath-cs!dcl-cs!bath.ac.uk!bspwrb
From: bspwrb@bath.ac.uk (W R Bennett)
Subject: Male and female symbols in Microsoft Word
Organization: School of Biological Sciences, University of Bath, UK
Message-ID: <E51wos.3sF.B.midge@bath.ac.uk>
Date: Mon, 3 Feb 1997 23:46:04 GMT
Lines: 16

Hi,

Does anyone know if there exist male and female symbols lurking in some
obscure corner of Word 7.0 for Win95?  This would be rather useful for
writing papers on mouse genetics, but four of us have now wasted hours
rummaging through the fonts to no avail.  Can anyone help? 

Many thanks,

Bill Bennett
Developmental Biology Unit, School of Biology and Biochemistry
University of Bath

bspwrb@bath.ac.uk



From owner-software@net.bio.net Tue Feb 04 22:00:00 1997
Path: biosci!BGUMAIL.BGU.AC.IL!aflaloc
From: aflaloc@BGUMAIL.BGU.AC.IL (Claude Aflalo)
Newsgroups: bionet.software
Subject: Re: Restriction Enzymes in DNA Strider?
Date: 4 Feb 1997 23:34:34 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 78
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <Pine.SGI.3.91.970205093331.15987B-100000@bgumail.bgu.ac.il>
References: <nc1-0402971130080001@phanes94.mc.duke.edu>
NNTP-Posting-Host: net.bio.net

On 4 Feb 1997, Namjin Chung wrote:

> Dear DNA Strider Users:
> 
> I use the above software for some sequence processing, and I think it's
> simple but very good software.  The only one thing I'm not satisfied with
> it is that it would not allow custom restriction by a selected set of
> REs.  While MacVector allows you to cut DNA with the enzyme set you
> currently have, it is big and key-locked.
> 
> Does anybody know how to solve this problem?  TIA.
> 
> 
> Namjin Chung, who knows little and asks a lot!
> Program in Molecular Cancer Biology
> Duke University Medical Center
> Durham, North Carolina
> 
Here is a tips collection which will answer that

To select one or a few enzymes on strider:  Hold down the option key
when selecting digest, or graphic map.  This will bring up another
menu.  At the bottom of the menu there is a choice between triple,
double, unique, or none.  Select none.  To select a specific enzyme,
click on individual enzymes in the enzyme list.  To select more than
one, hold down the aplle (command?) key while selecting the enzyme.

_____________________________________

: In article <jpcd0-1602951411250001@macr1-4.welc.cam.ac.uk>,
: jpcd0@mole.bio.cam.ac.uk (John Dixon) writes:
: > P.S. BTW, does anyone know if it is possible to deselect enzymes
: > when generating a full restriction map, to avoid the huge stacks of
: > four cutters above the sequence and the enormous list of tiddly
: > fragments in the site analysis below?
:
: The only way I know of doing this is to delete enzymes manually from your
: RE
: data file.  In the same way, you can add new enzymes.
:
: - I tried modifying this file when I had the original problem, using
:   the word processor that it was originally created in, and had problems
:   with Strider crashing. Do you have any hints about how to modify it?
:   e.g. What word processor/editor do you use, and what format do you
:   choose to save it in?

I have problems editing the RE library file.  I just load it into
SimpleText or Teachtext (its predecessor) and edit away. The format goes
like:-

Spe I,      a/ctagt,
Sph I,      gcatg/c,

Taq I,      t/cga,
 (etc)
%%%%%%

; comment comment comment

This could also be done in a word processor such as Word as long as you
save it in plain text (ASCII).
Niggle/Problem
The only problem arises with the fact that I want 2 RE libraries, one for
all the enzymes in the freezer and one for all those we could buy if
needed (ie the NEB one supplied.  I keep these as two files "RE library
NEB" and "RE library -20".  To use one of these erase "RE library", I
duplicate the desired file, rename that duplicate "RE library" and run
Strider.
-----------------------------



Claude Aflalo ######################## Phones: Office 972-7-6472118 ####
Dept. of Life Sciences				Lab   972-7-6472119
Ben Gurion University of the Negev	        Fax   972-7-6472890
P.O.Box 653				email:  aflaloc@bgumail.bgu.ac.il
Beer Sheva 84105 Israel	       URL: http://www.bgu.ac.il/life/aflalo.html


From owner-software@net.bio.net Tue Feb 04 22:00:00 1997
Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!cpk-news-feed2.bbnplanet.com!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!howland.erols.net!usc!comserv-g-01.usc.edu!user
From: t@hsc.edu
Newsgroups: bionet.software
Subject: Re: Restriction Enzymes in DNA Strider?
Date: Tue, 04 Feb 1997 21:01:03 +0100
Organization: University of Southern California, Los Angeles, CA
Lines: 28
Sender: tianjian@comserv-g-01.usc.edu
Message-ID: <t-0402972101030001@comserv-g-01.usc.edu>
References: <nc1-0402971130080001@phanes94.mc.duke.edu>
NNTP-Posting-Host: comserv-g-01.usc.edu

Dear Chung:
       DNA Strider has the function for you to select a set of enzymes.
Just hold the "Option" and select the "graphic Map" within the "Enz".
Then, select the enzyme names (by hold "shift" to do multiple selection).
Try it.

Tian-Jian Chen
Fellow in Clinic Molecular Genetics
CHLA 

In article <nc1-0402971130080001@phanes94.mc.duke.edu>, nc1@acpub.duke.edu
(Namjin Chung) wrote:

> Dear DNA Strider Users:
> 
> I use the above software for some sequence processing, and I think it's
> simple but very good software.  The only one thing I'm not satisfied with
> it is that it would not allow custom restriction by a selected set of
> REs.  While MacVector allows you to cut DNA with the enzyme set you
> currently have, it is big and key-locked.
> 
> Does anybody know how to solve this problem?  TIA.
> 
> 
> Namjin Chung, who knows little and asks a lot!
> Program in Molecular Cancer Biology
> Duke University Medical Center
> Durham, North Carolina

From owner-software@net.bio.net Tue Feb 04 22:00:00 1997
Path: biosci!agate!spool.mu.edu!uwm.edu!newsfeeds.sol.net!europa.clark.net!news.bbnplanet.com!cpk-news-hub1.bbnplanet.com!cpk-news-feed1.bbnplanet.com!uky.edu!usenet
From: yliu0@pop.uky.edu
Newsgroups: bionet.software
Subject: Re: Male and female symbols in Microsoft Word
Date: Wed, 05 Feb 1997 09:20:51 GMT
Organization: University of Kentucky Computing Services
Lines: 38
Message-ID: <5d98ut$i6e@service3.uky.edu>
References: <E51wos.3sF.B.midge@bath.ac.uk>
NNTP-Posting-Host: node-01-10.dialin.uky.edu
X-Newsreader: Forte Free Agent 1.0.82

It is inside word 7.0. 

Go to menu "insert" -> "symbol". From font list choose
"CommonBullets", male and female symbols are inside.


----------------------------------------------------------------------------

Department of Chemistry        Phone :(606) 254-9375
University of  Kentucky             LIU   YUE       

Email :   yliu0@pop.uky.edu	      
---------------------------------------------------------------------------
HOMEPAGE    Personal:sac.uky.edu/~yliu0
                             Software:www.engr.uky.edu/~yliu0
---------------------------------------------------------------------------


bspwrb@bath.ac.uk (W R Bennett) wrote:

>Hi,

>Does anyone know if there exist male and female symbols lurking in some
>obscure corner of Word 7.0 for Win95?  This would be rather useful for
>writing papers on mouse genetics, but four of us have now wasted hours
>rummaging through the fonts to no avail.  Can anyone help? 

>Many thanks,

>Bill Bennett
>Developmental Biology Unit, School of Biology and Biochemistry
>University of Bath

>bspwrb@bath.ac.uk





From owner-software@net.bio.net Tue Feb 04 22:00:00 1997
Path: biosci!rutgers!news.columbia.edu!panix!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!uunet!in2.uu.net!204.238.120.21!jump.net!grunt.dejanews.com!not-for-mail
Date: Wed, 05 Feb 1997 12:11:06 -0600
From: sxr3008@megahertz.njit.edu
Subject: ms word to html
Newsgroups: alt.hypertext,alt.windows95,alt.html,bionet.software
Message-ID: <855165652.25149@dejanews.com>
Organization: New Jersey Institute of Technology
To: sxr3008@megahertz.njit.edu
http: //megahertz.njit.edu/~sxr3008
X-Article-Creation-Date: Wed Feb 05 18:00:52 1997 GMT
X-Originating-IP-Addr: 128.235.228.144 (ppp-228-144.njit.edu)
X-Http-User-Agent: Mozilla/3.01Gold (Win95; I)
X-Authenticated-Sender: sxr3008@megahertz.njit.edu
Lines: 13

Hi, there:

I am trying to write my own word-to-html converter. But I don't know
how the MS word's .doc file is formated. There must be some format
specification like html's tags or DTD. But I have no idea where to find
it out. Can anybody give some hints?

TIA.

Shaomei

-------------------==== Posted via Deja News ====-----------------------
      http://www.dejanews.com/     Search, Read, Post to Usenet

From owner-software@net.bio.net Tue Feb 04 22:00:00 1997
Path: biosci!UCONNVM.UCONN.EDU!knecht
From: knecht@UCONNVM.UCONN.EDU (David Knecht)
Newsgroups: bionet.software
Subject: Seqvu
Date: 5 Feb 1997 09:16:55 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 16
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <l03020305af1e2cfa43e5@[137.99.27.55]>
NNTP-Posting-Host: net.bio.net

We have found Seqvu to be an excellent companion to Clustal in terms of
viewing and manipulating sequence alignments on the Macintosh.  When I
tried to register the program, the Garvan INstitute (Sydney, AU, which
claims responsibility on the splash screen) either no longer exists or no
longer supports the program. Does anyone know if someone is supporting it?
Is there a good alternative anyone knows of for viewing and tweeking
alignments?  Thanks- Dave

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
U-125
Storrs, CT 06269
Knecht@uconnvm.uconn.edu



From owner-software@net.bio.net Tue Feb 04 22:00:00 1997
Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!worldnet.att.net!news.maxwell.syr.edu!news.bc.net!uvaix3e1.comp.UVic.CA!hera.bioc.uvic.ca!cupton
From: cupton@uvic.ca (Chris Upton)
Newsgroups: bionet.molbio.proteins,bionet.software
Subject: pI calculation
Followup-To: bionet.molbio.proteins
Date: Wed, 5 Feb 97 00:49:28 GMT
Organization: University of Victoria
Lines: 22
Message-ID: <cupton.1205491408G@news.uvic.ca>
NNTP-Posting-Host: hera.bioc.uvic.ca
X-Newsreader: VersaTerm Link v1.1.6
Xref: biosci bionet.molbio.proteins:9910 bionet.software:17787

Hi,
  I'm looking for a way to query a protein database (eg Swiss-Prot) by pI.
ie get all proteins with pI > 8.

I'm not sure if there's programs to allow calc of pI for all proteins in the
database.  I guess output file could be large :-)

Then I might want to sort them....

Hmm, alternatively it would be nice to at least be able to get the pI for
1-200 proteins. Is there anything that will allow batch processing?
I seem to think I did this in the very distant past with 200 proteins,
either with GCG or IG.

Any advice appreciated....

Cheers,
  Chris Upton
Chris Upton
Biochemistry & Microbiology
University of Victoria          phone  250-721-6507
BC Canada                       fax    250-721-8855

From owner-software@net.bio.net Tue Feb 04 22:00:00 1997
Path: biosci!rutgers!uwm.edu!news-peer.gsl.net!news.gsl.net!mr.net!newshub.tc.umn.edu!lynx.unm.edu!bubba.NMSU.Edu!usenet
From: "Donald D. Bustamante" <dbustama@psl.nmsu.edu>
Newsgroups: bionet.software
Subject: Re: Male and female symbols in Microsoft Word
Date: Tue, 04 Feb 1997 10:10:13 -0800
Organization: National Biotechnology Information Facility
Lines: 28
Message-ID: <32F77B85.FFC@psl.nmsu.edu>
References: <E51wos.3sF.B.midge@bath.ac.uk>
NNTP-Posting-Host: 128.123.33.156
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.01Gold (WinNT; I)

W R Bennett wrote:
> 
> Hi,
> 
> Does anyone know if there exist male and female symbols lurking in some
> obscure corner of Word 7.0 for Win95?  This would be rather useful for
> writing papers on mouse genetics, but four of us have now wasted hours
> rummaging through the fonts to no avail.  Can anyone help?
> 
> Many thanks,
> 
> Bill Bennett
> Developmental Biology Unit, School of Biology and Biochemistry
> University of Bath
> 
> bspwrb@bath.ac.uk


Select "Insert" from the pull down menu, then symbol.  Change the font
to "Bookshelf Symbol 3"
-- 
Donald D. Bustamante, Project Manager
National Biotechnology Information Facility
Physical Science Laboratory
Box 30002, Dept. 3548
Las Cruces, New Mexico USA  88003
(505)-522-9209    FAX:  (505)-522-9434
URL:  http://www.nbif.org

From owner-software@net.bio.net Tue Feb 04 22:00:00 1997
Path: biosci!daresbury!nntp-trd.UNINETT.no!online.no!sn.no!nntp.uio.no!newsfeeds.sol.net!feed1.news.erols.com!howland.erols.net!surfnet.nl!newsfeed.sunet.se!news01.sunet.se!sunic!02-newsfeed.univie.ac.at!03-newsfeed.univie.ac.at!news.univie.ac.at!news-admin@univie.ac.at
From: "Gunther Rezniczek" <gr@abc.univie.ac.at>
Newsgroups: bionet.software
Subject: Re: Plasmid drawing software for PC??
Date: 5 Feb 1997 10:58:56 GMT
Organization: University of Vienna
Lines: 26
Message-ID: <01bc1353$94c9d5c0$48518283@Vectra-5122-CBL.abc.univie.ac.at>
References: <Pine.OSF.3.91.970203140529.30711C-100000@ermine.ox.ac.uk>
NNTP-Posting-Host: vectra-5122-cbl.abc.univie.ac.at
X-Newsreader: Microsoft Internet News 4.70.1155



Pita Enriquez Harris <enriquez@ermine.ox.ac.uk> wrote in article
<Pine.OSF.3.91.970203140529.30711C-100000@ermine.ox.ac.uk>...
> 
> 
> Does anyone know of a good program for drawing/managing DNA constructs on

> a PC?
> 
> If you know of where I can get a free download of a demo, that would be 
> especially helpful!
> 
> Thanks,
> Pita

There is a program called WinPlas 2.5 from Rich Goldstein.
It is easy to use and produces good output.
A demo can be downloaded from the web ... I can't remember the URL, but
just search the net for WinPlas and/or Goldstein and you should have no
trouble.

Hope this helps,

Gunther.
 

From owner-software@net.bio.net Tue Feb 04 22:00:00 1997
Path: biosci!BGUMAIL.BGU.AC.IL!raveh
From: raveh@BGUMAIL.BGU.AC.IL (Dina Raveh BGU)
Newsgroups: bionet.software
Subject: (none)
Date: 5 Feb 1997 01:38:18 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 11
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <Pine.SGI.3.91.970205113838.24913A-100000@bgumail.bgu.ac.il>
NNTP-Posting-Host: net.bio.net

 subscribe bio-soft@net.bio.net


**********************************
Dina Raveh
Department of Life Sciences
Ben Gurion University of the Negev
Beersheva, 84105 Israel
Tel. 972-7-6461371
Fax. 972-7-6472992 


From owner-software@net.bio.net Tue Feb 04 22:00:00 1997
Path: biosci!rutgers!gatech!csulb.edu!hammer.uoregon.edu!news-xfer.netaxs.com!news.maxwell.syr.edu!news.bc.net!torn!butler.ac.BrockU.CA!mcuggy
From: mcuggy@PROBLEM_WITH_INEWS_GATEWAY_FILE (Michael Cuggy)
Newsgroups: bionet.software
Subject: rarefraction
Date: 5 Feb 1997 20:49:18 GMT
Organization: Brock University
Lines: 4
Message-ID: <5darof$6ve@butler.ac.BrockU.CA>
NNTP-Posting-Host: craton.geol.brocku.ca
X-Newsreader: TIN [version 1.2 PL2]

Hi I need to find a program to do rarefraction on the Mac. Can anyone
point me where to look. Please reply by e-mail if possible. Thank you.

Michael Cuggy

From owner-software@net.bio.net Tue Feb 04 22:00:00 1997
Path: biosci!rutgers!news-relay.ncren.net!newsgate.duke.edu!mmorales
From: mmorales@acpub.duke.edu (Michael Morales)
Newsgroups: bionet.software
Subject: Short Similarity Search
Date: 5 Feb 1997 22:22:43 GMT
Organization: Duke University, Durham, NC, USA
Lines: 9
Message-ID: <5db17j$jl8@newsgate.duke.edu>
NNTP-Posting-Host: bio6.acpub.duke.edu
X-Newsreader: TIN [version 1.2 PL2]

I am performing in situ hybridization using short (35-50 nucleotides)
oligonucleotide probes.  I want to check the databases by FASTA and BLAST for
other sequences that may have similarity to these.  Are there any special
settings or programs that are optimal for use with short sequences?  Any
favorite sites?  Any help would be greatly appreciated.

Mike Morales
 


From owner-software@net.bio.net Tue Feb 04 22:00:00 1997
Path: biosci!rutgers!gatech!EU.net!Norway.EU.net!nntp.uio.no!in1.nntp.cais.net!news.us.net!usenet
From: Jeff Marshall <jeff@infocomm.net>
Newsgroups: alt.hypertext,alt.windows95,alt.html,bionet.software
Subject: Re: ms word to html
Date: Wed, 05 Feb 1997 13:51:03 -0500
Organization: US Net - MD,DC,VA ISP - info@us.net
Lines: 35
Message-ID: <32F8D697.432B@infocomm.net>
References: <855165652.25149@dejanews.com>
Reply-To: jeff@infocomm.net
NNTP-Posting-Host: 206.225.29.5
Mime-Version: 1.0
Content-Type: multipart/alternative; boundary="----------55913A393D027"
X-Sender: Jeff Marshall <jeff@infocomm.net> (Unverified)
X-Mailer: Mozilla 4.0b1 (WinNT; I)
X-Priority: Normal


------------55913A393D027
Content-Transfer-Encoding: 7bit
Content-Type: text/plain; charset=us-ascii

Hi,

Word has an okay HTML assistant that already converts Word text to
HTML.  If you haven't looked at that yet, you may want to go over to
http://microsoft.com and get the assistant.

Jeff Marshall

------------55913A393D027
Content-Transfer-Encoding: 7bit
Content-Type: text/html; charset=us-ascii

<HTML><BODY>

<DT>Hi,</DT>

<DT>&nbsp;</DT>

<DT>Word has an okay HTML assistant that already converts Word text to
HTML.&nbsp; If you haven't looked at that yet, you may want to go over
to http://microsoft.com&nbsp;and get the assistant.</DT>

<DT>&nbsp;</DT>

<DT>Jeff Marshall</DT>

</BODY>
</HTML>
------------55913A393D027--


From owner-software@net.bio.net Tue Feb 04 22:00:00 1997
Path: biosci!IAFRICA.COM!ccantina
From: ccantina@IAFRICA.COM ("C. Cantina")
Newsgroups: bionet.software
Subject: Chemical structures
Date: 5 Feb 1997 12:34:46 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 11
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <32F977DA.3344@iafrica.com>
Reply-To: ccantina@iafrica.com
NNTP-Posting-Host: net.bio.net

Hi there,

does anyone know of a program which allows one to draw chemical
structures, including DNA/RNA sequences? I would like to find one if
posible which works with Word Perfect.

Please, inform me if someone knows where to find this software.
Thanks

C Cantina


From owner-software@net.bio.net Tue Feb 04 22:00:00 1997
Path: biosci!rutgers!gatech!csulb.edu!hammer.uoregon.edu!news1.mpcs.com!news.radio.cz!voskovec.radio.cz!news.radio.cz!CESspool!news.apfel.de!fu-berlin.de!news-ber1.dfn.de!news-ham1.dfn.de!news-han1.dfn.de!newsserver.rrzn.uni-hannover.de!tubsibr!rzlimes.gbf-braunschweig.de!news
From: reichelt@gbf-braunschweig.de (Joachim Reichelt)
Newsgroups: bionet.software,bionet.molec-model
Subject: Re: Program for molecular modeling.
Date: 5 Feb 1997 13:50:59 GMT
Organization: GBF Braunschweig
Lines: 16
Message-ID: <5da383$a0s@rzlimes.gbf-braunschweig.de>
References: <Pine.OSF.3.95.970125173803.2874B-100000@saturn.mahidol.ac.th>
NNTP-Posting-Host: msfjre.gbf-braunschweig.de
Mime-Version: 1.0
Content-Type: Text/Plain; charset=US-ASCII
X-Newsreader: WinVN 0.99.6
Xref: biosci bionet.software:17796 bionet.molec-model:1357

In article <Pine.OSF.3.95.970125173803.2874B-100000@saturn.mahidol.ac.th>, 
u3601087@mahidol.ac.th says...
>
>Dear all,
> I wold like to know the name of the software other than from MSI and
>Waht_if. Are there any that can do molecular modeling for protein?
>                                                Thank you in advance,
>
Have a look at our page for BRAGI at :

http://www.gbf-braunschweig.de/zwe/MSF/bragi_group/Bragie.html

BRAGI is free to academics.

best wishes,


From owner-software@net.bio.net Wed Feb 05 22:00:00 1997
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!news.maxwell.syr.edu!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!howland.erols.net!panix!cmcl2.nyu.edu!news.nyu.edu!not-for-mail
From: coetzeew@is2.nyu.edu
Newsgroups: bionet.software
Subject: Re: (none)
Date: Thu, 06 Feb 1997 13:22:43 -0500
Organization: New York University
Lines: 5
Message-ID: <32FA2173.6FC7@is2.nyu.edu>
References: <199702060315.OAA10453@anugpo.anu.edu.au>
NNTP-Posting-Host: mcpd44.med.nyu.edu
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.0 (Win95; I)

Shoba.Ranganathan wrote:
> 
> help

With what?

From owner-software@net.bio.net Wed Feb 05 22:00:00 1997
Path: biosci!daresbury!bioftp.unibas.ch!news.vub.ac.be!news.belnet.be!swsbe6.switch.ch!scsing.switch.ch!elna.ethz.ch!retrograde.ethz.ch!user
From: mantei@neuro.biol.ethz.ch (Ned Mantei)
Newsgroups: bionet.software,bionet.molbio.methds-reagnts
Subject: Re: Restriction Enzymes in DNA Strider?
Date: Thu, 06 Feb 1997 09:37:53 +0100
Organization: Dept. of Neurobiology, ETH-Zurich
Lines: 20
Message-ID: <mantei-0602970937530001@retrograde.ethz.ch>
References: <nc1-0402971130080001@phanes94.mc.duke.edu>
NNTP-Posting-Host: retrograde.ethz.ch
Xref: biosci bionet.software:17808 bionet.molbio.methds-reagnts:54446

In article <nc1-0402971130080001@phanes94.mc.duke.edu>, nc1@acpub.duke.edu
(Namjin Chung) wrote:

> Dear DNA Strider Users:
> 
> I use the above software for some sequence processing, and I think it's
> simple but very good software.  The only one thing I'm not satisfied with
> it is that it would not allow custom restriction by a selected set of
> REs. 

If you hold down the option key while choosing "Graphic map..." from the
menu "Enz", you can choose from the restriction enzymes in the file
RELibrary.RELibrary can be edited by a word processor (save as "Text
only"). Or is this not what you meant?

-- 
Ned Mantei
Dept. of Neurobiology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland
mantei@neuro.biol.ethz.ch   Fax: +41-1-633-1046

From owner-software@net.bio.net Wed Feb 05 22:00:00 1997
Path: biosci!agate!spool.mu.edu!howland.erols.net!rill.news.pipex.net!pipex!oleane!pasteur.fr!jussieu.fr!univ-lyon1.fr!news
From: bioch@lnsd-res.univ-lyon1.fr (biochimie)
Newsgroups: bionet.software
Subject: Re: Rapd analysis
Date: 6 Feb 1997 10:09:05 GMT
Organization: INSERM U189
Lines: 31
Message-ID: <5dcak1$2sb@tempo.univ-lyon1.fr>
References: <Pine.OSF.3.95.970131194345.4344A-100000@saturn.mahidol.ac.th> <5ctb87$m3r@news.csus.edu>
NNTP-Posting-Host: pc-bioch2.univ-lyon1.fr
Mime-Version: 1.0
Content-Type: Text/Plain; charset=ISO-8859-1
X-Newsreader: WinVN 0.99.5

In article <5ctb87$m3r@news.csus.edu>, sac30867@saclink2.csus.edu says...
>
>Panapat Uawithya (u3601087@mahidol.ac.th) wrote:
>:  Are there any software that can 
>:  1 analyze for rapd
>:  3 If possible freeware or shareware.
>:  4 PC or UNIX
>
>the RAPD analysis package runs in DOS and is free and (surprise) analyzes 
>RAPDs.  I forgot the name of the file, but it is available from the 
>SimTel archives in the biology directory.  The latest version (i think) 
>is 1.04.
>
>NIH-Image PC is available in beta test from somewhere.  I'm still trying 
>to get it to analyze a gel for me, so I can't comment on how well it 
>works (yet!).  It, also, is free & runs under Win95.
You can get ImagePC on the Web site Of Scion Corporation at the following 
address :
http://www.scioncorp.com/Image

P.GEORGE
-- 
INSERM U189
Laboratoire de BIOCHIMIE
Faculte de Medecine LYON-SUD
BP 12 
69921 OULLINS CEDEX
Tel : 78-86-31-58
Fax : 78-50-71-52
Directeur : Pr Pierre LOUISOT


From owner-software@net.bio.net Wed Feb 05 22:00:00 1997
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!www.nntp.primenet.com!nntp.primenet.com!news1.best.com!nntp2.ba.best.com!jdulaney
From: jdulaney@nntp.best.com (John Dulaney)
Newsgroups: bionet.software
Subject: NEED: UNLIMITED INSTALLER or NEVERLOCK or similar
Date: 6 Feb 1997 06:13:10 GMT
Lines: 1
Message-ID: <5dbspm$imf$3@nntp2.ba.best.com>
NNTP-Posting-Host: shellx.best.com
X-Newsreader: TIN [version 1.2 PL2]

E-mail please. john

From owner-software@net.bio.net Wed Feb 05 22:00:00 1997
Path: biosci!ANU.EDU.AU!sxr224
From: sxr224@ANU.EDU.AU ("Shoba.Ranganathan")
Newsgroups: bionet.software
Subject: (none)
Date: 5 Feb 1997 19:15:23 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 1
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199702060315.OAA10453@anugpo.anu.edu.au>
Reply-To: Shoba.Ranganathan@anu.edu.au
NNTP-Posting-Host: net.bio.net

help

From owner-software@net.bio.net Wed Feb 05 22:00:00 1997
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!www.nntp.primenet.com!nntp.primenet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!cpk-news-hub1.bbnplanet.com!worldnet.att.net!news.u.washington.edu!root
From: "R. Henne" <henne@u.washington.edu>
Newsgroups: bionet.software
Subject: Re: Chemical structures
Date: Wed, 05 Feb 1997 17:59:05 -0800
Organization: University of Washington
Lines: 23
Message-ID: <32F93AE9.2781@u.washington.edu>
References: <32F977DA.3344@iafrica.com>
NNTP-Posting-Host: beatrice01.u.washington.edu
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
NNTP-Posting-User: henne
X-Mailer: Mozilla 2.02 (X11; I; AIX 2)

C. Cantina wrote:
> 
> Hi there,
> 
> does anyone know of a program which allows one to draw chemical
> structures, including DNA/RNA sequences? I would like to find one if
> posible which works with Word Perfect.
> 
> Please, inform me if someone knows where to find this software.
> Thanks
> 
> C Cantina

I like Isis Draw.  It comes with nucleotide and amino acid structures.
It is free for academic and personal use.  It also plays well with other
Windows apps.  There is also a Mac version at the same site. It worked
with MS Word but I haven't tried it with Word Perfect.

Here is the URL:

http://www.mdli.com/prod/ioffer.html

Randy

From owner-software@net.bio.net Wed Feb 05 22:00:00 1997
Path: biosci!CSU.edu.au!jwilkinson
From: jwilkinson@CSU.edu.au ("Jenny Wilkinson")
Newsgroups: bionet.software
Subject: Re: Male and female symbols in Microsoft Word
Date: 5 Feb 1997 16:30:01 -0800
Organization: Charles Sturt University - Riverina
Lines: 11
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <BBA81A76BA7@whealth.riv.csu.edu.au>
NNTP-Posting-Host: net.bio.net


It is inside word 7.0. 

Go to menu "insert" -> "symbol". From font list choose
"CommonBullets", male and female symbols are inside.

How about in Word 6? or are these symbols only in Word 7.

jenny



From owner-software@net.bio.net Wed Feb 05 22:00:00 1997
Path: biosci!agate!spool.mu.edu!uwm.edu!www.nntp.primenet.com!nntp.primenet.com!howland.erols.net!portc02.blue.aol.com!audrey01.news.aol.com!not-for-mail
From: zevsolltec@aol.com (ZevSolltec)
Newsgroups: bionet.software
Subject: Senior Programmer Analyst Needed
Date: 6 Feb 1997 19:42:59 GMT
Organization: AOL http://www.aol.com
Lines: 14
Message-ID: <19970206194200.OAA14800@ladder01.news.aol.com>
NNTP-Posting-Host: ladder01.news.aol.com
X-Admin: news@aol.com

Senior Programmer Analyst

Plan and execute major, complex programming projects related to medical
and scientific imaging on Macintosh, Windows 95 and NT platforms. 
Requires a BS in computer science, three years experience programming in C
or C++; knowledge of basic hardware architecture and add-on devices,
specifically those used in image acquisition such as frame-grabber boards;
or equivalent.  Desirable: Master degree, and knowledge of biological
sciences.  Salary Commensurate.  Send resume to Zev Sunleaf, Department of
Biological Sciences, 138 Biology Building, University of Iowa, Iowa City,
IA 52242-1324.  The University of Iowa is an Affirmative Action/Equal
Opportunity Employer.  Women and minority candidates are encouraged to
apply.


From owner-software@net.bio.net Wed Feb 05 22:00:00 1997
Path: biosci!agate!howland.erols.net!ix.netcom.com!news.enteract.com!news.inetnebr.com!netserv.unmc.edu!netserv!gspahwa
From: gspahwa@netserv.unmc.edu (Gurcharan Pahwa)
Newsgroups: bionet.software
Subject: GCG
Date: 6 Feb 1997 17:36:40 GMT
Organization: University of Nebraska Medical Center
Lines: 10
Message-ID: <5dd4r8$7v3@netserv.unmc.edu>
NNTP-Posting-Host: 137.197.98.10
X-Newsreader: TIN [version 1.2 PL2]

Wisconsin Sequence Analysis Package from GCG is a Unix based sequence
analysis software and has most of the features of Macvector and other PC
compatible sequence analysis softwares.
E-mail doc@gcg.com
http://www.gcg.com
They have graphical user,s interface. Surf their Web site.

Disclaimer: THis is in response to your query and not the promotion of the
product.


From owner-software@net.bio.net Wed Feb 05 22:00:00 1997
Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!www.nntp.primenet.com!nntp.primenet.com!howland.erols.net!bloom-beacon.mit.edu!news.starnet.net!news.starnet.net!news.utdallas.edu!news.swmed.edu!news
From: Mark Walberg <walberg@simmons.swmed.edu>
Newsgroups: bionet.software,bionet.molbio.methds-reagnts
Subject: Re: Restriction Enzymes in DNA Strider?
Date: Thu, 06 Feb 1997 11:03:05 +0000
Organization: UT Southwestern Medical Center
Lines: 15
Message-ID: <32F9BA6A.4764@simmons.swmed.edu>
References: <nc1-0402971130080001@phanes94.mc.duke.edu>
Reply-To: walberg@simmons.swmed.edu
NNTP-Posting-Host: scc17.swmed.edu
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.0 (Macintosh; I; 68K)
Xref: biosci bionet.software:17812 bionet.molbio.methds-reagnts:54474

Namjin Chung wrote:
> 
> Dear DNA Strider Users:
> The only one thing I'm not satisfied with
> it is that it would not allow custom restriction by a selected set of
> REs.  
> Does anybody know how to solve this problem?  TIA.
> Namjin Chung, who knows little and asks a lot!
> Program in Molecular Cancer Biology
> Duke University Medical Center
> Durham, North Carolina

Hold the option key down when you select the Graphic Map.  This will
give you a window allowing you to select any enzyme that you have in
your RE library.  -MW

From owner-software@net.bio.net Wed Feb 05 22:00:00 1997
Path: biosci!agate!nntpfeed.doc.ic.ac.uk!sunsite.doc.ic.ac.uk!warwick!leicester!pc5
From: ray@le.ac.uk (Raymond Dalgleish)
Newsgroups: bionet.software
Subject: Re: Male and female symbols in Microsoft Word
Date: Thu, 06 Feb 97 11:40:34 GMT
Organization: Department of Genetics, University of Leicester, UK
Lines: 22
Message-ID: <5dcfvi$d58_001@gene.le.ac.uk>
References: <BBA81A76BA7@whealth.riv.csu.edu.au>
NNTP-Posting-Host: pc5.gene.le.ac.uk
X-Newsreader: News Xpress Version 1.0 Beta #4

In article <BBA81A76BA7@whealth.riv.csu.edu.au>,
   jwilkinson@CSU.edu.au ("Jenny Wilkinson") wrote:
>
>It is inside word 7.0. 
>
>Go to menu "insert" -> "symbol". From font list choose
>"CommonBullets", male and female symbols are inside.
>
>How about in Word 6? or are these symbols only in Word 7.
>
>jenny
>
Microsoft produced a set of true type fonts containing mostly
symbols that aided in the conversion of Word Perfect documents
to Word. The fonts (five of them I think) can be downloaded
from Microsoft's web site. The file might be available elsewhere
as wpcnv.exe . The female and male symbols are in the font called
Iconic Symbols Ext.

Enjoy!

Raymond Dalgleish

From owner-software@net.bio.net Wed Feb 05 22:00:00 1997
Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!cpk-news-hub1.bbnplanet.com!newsfeed.internetmci.com!demos!news.florin.ru!usenet
From: Konstantin Savov <kps@florin.ru>
Newsgroups: bionet.software
Subject: improved Web access to botanical databases
Date: Thu, 06 Feb 1997 21:59:57 -0500
Organization: DataX/FLORIN, Inc.
Lines: 32
Message-ID: <32FA9AAD.15FB@florin.ru>
NNTP-Posting-Host: alpha.florin.ru
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.01 (X11; I; OSF1 V4.0 alpha)

DataX/FLORIN ANNOUNCES
              IMPROVED WEB ACCESS TO BOTANICAL DATABASES

Web access to the databases published on our site has been adjusted
with a new revision of FLORIN Taxonomy Web Application.  The latter is
customizable for needs of a particular database now.

The following databases are currently available on
http://www.florin.ru/florin/db/:
        - Catalogue of Authentic Specimens of Moscow State University
          Herbarium (MW);
        - Mosses of the former USSR, by Dr. Michael Ignatov;
        - A Taxonomic Revision of Pseudodracontium (Araceae - Aroideae -
          Thomsonieae), by Dr.  Michael Serebryanyi;
        - Lotus database, by Dr. Tatyana Kramina.

Now, you may
        - search for data using customized query forms,
        - get lists of taxa and browse through them without filling in
          a query form,
        - get reports (synopsis, taxonomic tree) generated from a
          database by clicking one button.

Any feedback is appreciated.  Thank you in advance.

-------------------------------------------------------
Konstantin Savov                E-mail: kps@florin.ru
FLORIN Project Leader           Voice:  +7(095)158-9520
                                Fax:    +7(095)158-5700
DataX/FLORIN, Inc.              Moscow, Russia
-------------------------------------------------------
   FLORIN Home Page: <http://www.florin.ru/florin/>

From owner-software@net.bio.net Wed Feb 05 22:00:00 1997
Path: biosci!agate!spool.mu.edu!uwm.edu!newsfeeds.sol.net!feed1.news.erols.com!howland.erols.net!news.nacamar.de!fu-berlin.de!news.belwue.de!News.Uni-Marburg.DE!not-for-mail
From: klieber@mailer.uni-marburg.de (Hans-Georg Klieber)
Newsgroups: bionet.software
Subject: What is your favorite patch-clamp hardware/software?
Date: Thu, 06 Feb 1997 08:16:16 GMT
Organization: University of Marburg, Germany
Lines: 25
Message-ID: <32f99325.3279390@news.uni-marburg.de>
NNTP-Posting-Host: mppp029.ppp.uni-marburg.de
X-Newsreader: Forte Free Agent 1.1/32.230

Dear colleagues,

I would like to collect your opinions on the advanteges
and disadvanteges of the electrophysiology equipment
that you use for patch-clamping.

I am particularly interested in your experience regarding
- amplifiers 
- computer controlled measurement (whole cell, single
channel), hardware & software
- choice of mass storage
- software to further evaluate the measurments

Your responses are greatly appreciated. 
I will provide a summary of all contributions.

Thank you for your help,
Hans-Georg Klieber

--
Dr. Hans-Georg Klieber, klieber@mailer.uni-marburg.de
Physiological Institute, University of Marburg, Germany
Office: +49-6421-286521, Home: -25680
http://staff-www.uni-marburg.de/~klieber/


From owner-software@net.bio.net Thu Feb 06 22:00:00 1997
Path: biosci!rutgers!gatech!news.sprintlink.net!news-peer.sprintlink.net!howland.erols.net!math.ohio-state.edu!jussieu.fr!univ-lyon1.fr!news.univ-aix.fr!usenet
From: Daniel GAUTHERET <gauthere@gauss.cnrs-mrs.fr>
Newsgroups: bionet.molbio.evolution,bionet.general,bionet.software
Subject: Ph.D./Postdoc Position: Computational/wet Biology
Date: Fri, 07 Feb 1997 12:14:17 +0100
Organization: Universites d' Aix en Provence
Lines: 43
Message-ID: <32FB0E89.41C6@gauss.cnrs-mrs.fr>
NNTP-Posting-Host: gauss.cnrs-mrs.fr
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.0 (X11; I; IRIX 5.3 IP22)
Xref: biosci bionet.molbio.evolution:5634 bionet.general:25525 bionet.software:17820

=========== Computational/wet Biology on the French Riviera ===========
                             Feb 1997

The Laboratory of Structural & Genetic Information, (EP 91-CNRS)
of the Institute of Structural Biology & Microbiology (IBSM)
offers immediate doctoral and post-doctoral opportunities in the 
following areas of computational biology:
  - 1.  Analysis of ESTs and Genomic sequence data in Plants
  - 2.  Analysis of microbial genomes
  - 3.  Human genomics and human paleogenetics (study on "ancient DNA")

A solid background in Plant and/or Microbial biology is needed for
positions 1 & 2, as well as a working knowledge of the usual sequence 
analysis tools (GenBank, Blast, etc). Ideal candidates would have 
been previously involved in a sequencing project.

Candidates for position 3 should be prepared to equally divide their
work into wet and "in silico" biology. Again a working knowledge of
sequence analysis techniques is needed.

The laboratory is already well equipped in term of hardware, software
and
basic bioinformatics skills. We are now looking to reinforce our
biological/
genomics potential to take advantage of the computer systems now in
place.

More information on the activity of the laboratory can be found on our
internet server: http://igs-server.cnrs-mrs.fr 

Please address your CV and two references to:

================================================================
Jean-Michel Claverie, Dr. Sc.                jmc@igs.cnrs-mrs.fr
Tel:   +33 4 91 16 45 48                  Fax: +33 4 91 16 45 49
Director,
Laboratory of Structural & Genetic Information,     EP 91 - CNRS
Institute of Structural Biology & Microbiology (IBSM)
31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, FRANCE
================================================================

E-mail is preferred. Candidates from all countries of the EEC are
encouraged to apply.

From owner-software@net.bio.net Thu Feb 06 22:00:00 1997
Path: biosci!rutgers!gatech!EU.net!Norway.EU.net!nntp.uio.no!news.apfel.de!news-fra1.dfn.de!news-ber1.dfn.de!news-ham1.dfn.de!news-han1.dfn.de!newsserver.rrzn.uni-hannover.de!tubsibr!rzlimes.gbf-braunschweig.de!news
From: reichelt@gbf-braunschweig.de (Joachim Reichelt)
Newsgroups: bionet.software,bionet.molec-model
Subject: Re: Program for molecular modeling.
Date: 7 Feb 1997 09:29:24 GMT
Organization: GBF Braunschweig
Lines: 41
Message-ID: <5deslk$hi6@rzlimes.gbf-braunschweig.de>
References: <Pine.OSF.3.95.970125173803.2874B-100000@saturn.mahidol.ac.th> <5da383$a0s@rzlimes.gbf-braunschweig.de>
NNTP-Posting-Host: msfjre.gbf-braunschweig.de
Mime-Version: 1.0
Content-Type: Text/Plain; charset=US-ASCII
X-Newsreader: WinVN 0.99.6
Xref: biosci bionet.software:17821 bionet.molec-model:1359

In article <5da383$a0s@rzlimes.gbf-braunschweig.de>, 
reichelt@gbf-braunschweig.de says...
>
...
>Have a look at our page for BRAGI at :
>
>http://www.gbf-braunschweig.de/zwe/MSF/bragi_group/Bragie.html
Sorry, I had a Typo, please read it

http://www.gbf-braunschweig.de/zwe/MSF/bragi_group/BragiE.html

>
>BRAGI is free to academics.
>
>best wishes,
>

-- 
Mit freundlichen Gruessen                         Best Regards
 
         Joachim Reichelt
 
-------------------------------------------------------------------------
Joachim Reichelt
MSF - Zentrale Wiss. Einrichtung "Strukturforschung"
GBF  - Gesellschaft fuer Biotechnologische Forschung
Mascheroder Weg 1
D-38124 Braunscheig                            _/_/_/   _/_/_/   _/_/_/_/
                                             _/    _/  _/   _/  _/
Tel: +(49) 531 6181 352                     _/        _/   _/  _/
     +(49) 531 6181 224                    _/  _/    _/_/_/   _/_/_/
FAX: +(49) 531 6181 355                   _/    _/  _/   _/  _/
EMAIL:                                   _/    _/  _/   _/  _/
 REICHELT@gbf-braunschweig.de            _/_/_/   _/_/_/   _/
          
WWW:
 http://www.gbf-braunschweig.de/zwe/MSF/index.html
 http://www.gbf-braunschweig.de/zwe/MSF/bragi_group/Bragi.html
-- Disclaimer --
Standard > Keyword : Opinions, my own, nobody else's, whatsoever ...


From owner-software@net.bio.net Thu Feb 06 22:00:00 1997
Path: biosci!agate!howland.erols.net!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!su-news-hub1.bbnplanet.com!news.pbi.net!nntp.mainstreet.net!ns2.mainstreet.net!sloth.swcp.com!tesuque!lynx.unm.edu!bubba.NMSU.Edu!usenet
From: "Donald D. Bustamante" <dbustama@psl.nmsu.edu>
Newsgroups: alt.hypertext,alt.windows95,alt.html,bionet.software
Subject: Re: ms word to html
Date: Thu, 06 Feb 1997 07:43:25 -0800
Organization: National Biotechnology Information Facility
Lines: 27
Message-ID: <32F9FC1D.86@psl.nmsu.edu>
References: <855165652.25149@dejanews.com>
NNTP-Posting-Host: 128.123.33.156
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.01Gold (WinNT; I)

sxr3008@megahertz.njit.edu wrote:
> 
> Hi, there:
> 
> I am trying to write my own word-to-html converter. But I don't know
> how the MS word's .doc file is formated. There must be some format
> specification like html's tags or DTD. But I have no idea where to find
> it out. Can anybody give some hints?
> 
> TIA.
> 
> Shaomei
> 
> -------------------==== Posted via Deja News ====-----------------------
>       http://www.dejanews.com/     Search, Read, Post to Usenet


Word has the capability to do this.  It may be necessary to upgrade
your copy of Word; if so, it is available from http://www.microsoft.com
-- 
Donald D. Bustamante, Project Manager
National Biotechnology Information Facility
Physical Science Laboratory
Box 30002, Dept. 3548
Las Cruces, New Mexico USA  88003
(505)-522-9209    FAX:  (505)-522-9434
URL:  http://www.nbif.org

From owner-software@net.bio.net Thu Feb 06 22:00:00 1997
Path: biosci!bcm.tmc.edu!cs.utexas.edu!news-xfer.netaxs.com!feed1.news.erols.com!howland.erols.net!rill.news.pipex.net!pipex!warwick!lyra.csx.cam.ac.uk!news.ox.ac.uk!andrew.rambaut
From: andrew.rambaut@zoology.ox.ac.uk (Andrew Rambaut)
Newsgroups: bionet.software
Subject: Another Mac Multiple Alignment Program
Date: Fri, 07 Feb 1997 16:38:43 +0000
Organization: Zoology Department, University of Oxford
Lines: 23
Message-ID: <andrew.rambaut-ya023380000702971638430001@news.ox.ac.uk>
NNTP-Posting-Host: andyrmac.zoo.ox.ac.uk
Mime-Version: 1.0
Content-Type: text/plain; charset=ISO-8859-1
Content-Transfer-Encoding: 8bit
X-Newsreader: Yet Another NewsWatcher 2.3.3

I have written a multiple alignment editor for my own use due to
frustration about what was available so far. I use the current
version all the time and find it very useful. I think its best
feature is the ability to translate in real time from DNA to
amino acids whilst editing the alignment. Thus any changes you
make whilst looking at the AAs are actually made to the underlying
DNA sequences. There are loads of bugs and loads of features
still to be implemented. The manual is only half written. At
present I have no time to complete the project but if anyone
would like to take a look and try it out:

ftp://evolve.zoo.ox.ac.uk/packages/Se-Al/Se-Al10a1.hqx
http://evolve.zoo.ox.ac.uk/Se-Al/Se-Al/html

This is a FAT binary. Please, see what you think and get back to
me. I will try and help with any problems.

=====================================================================
  Andrew Rambaut,                                          
  Zoology Department,       EMAIL - Andrew.Rambaut@zoology.ox.ac.uk
  University of Oxford,  
  South Parks Road, Oxford, England.        
=====================================================================

From owner-software@net.bio.net Thu Feb 06 22:00:00 1997
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!www.nntp.primenet.com!nntp.primenet.com!EU.net!Germany.EU.net!main.Germany.EU.net!fu-berlin.de!unlisys!blackbush.xlink.net!scsing.switch.ch!rzunews.unizh.ch!newsadm
From: Rolf Kocherhans <rolfk@vetvir.unizh.ch>
Newsgroups: bionet.software
Subject: Beta testers wanted for molbio prg.
Date: Fri, 07 Feb 1997 10:27:04 +0100
Organization: University of Zurich, Switzerland
Lines: 28
Message-ID: <32FAF568.3EEA@vetvir.unizh.ch>
Reply-To: rolfk@vetvir.unizh.ch
NNTP-Posting-Host: 130.60.22.38
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.01 (Macintosh; I; PPC)

I need some beta testers to test some SuperCard projects which I made on
the Mac for the WWW. They are accessible over the WWW with the Roadster
plug in from Allegiant !

If you do not know what I talk about just connect to the URL, download
the plug in and you will be very surprised.

I have no access to IBM computers so I actually need some people testing
with the roadster plug in for windows.

You can find the plugin here:
http://www.allegiant.com/roadster/


You can find the page at:
http://www.unizh.ch/vetvir/programs.html


You can find two different programs there at the moment, one designs
Adaptors or Linkers and the other calculates Ligation dilutions.
The programs are very primitive and have no manual but they should be
easy to use.

Please let me know how they work in any case !

Cheers
Rolf
mailto:rolfk@vetvir.unizh.ch

From owner-software@net.bio.net Thu Feb 06 22:00:00 1997
Newsgroups: bionet.software
Path: biosci!rutgers!gatech!csulb.edu!hammer.uoregon.edu!news-peer.gsl.net!news.gsl.net!howland.erols.net!rill.news.pipex.net!pipex!uknet!usenet1.news.uk.psi.net!uknet!uknet!bcc.ac.uk!mol28.ioo.ucl.ac.uk!user
From: j.keen@ucl.ac.uk (Jeffrey Keen)
Subject: Re: Seqvu
Sender: news@ucl.ac.uk (Usenet News System)
Message-ID: <j.keen-0702971042410001@mol28.ioo.ucl.ac.uk>
Date: Fri, 7 Feb 1997 09:42:41 GMT
References: <l03020305af1e2cfa43e5@[137.99.27.55]>
Organization: Institute of Ophthalmology, London
Lines: 15

In article <l03020305af1e2cfa43e5@[137.99.27.55]>,
knecht@UCONNVM.UCONN.EDU (David Knecht) wrote:

> We have found Seqvu to be an excellent companion to Clustal in terms of
> viewing and manipulating sequence alignments on the Macintosh.  When I
> tried to register the program, the Garvan INstitute (Sydney, AU, which
> claims responsibility on the splash screen) either no longer exists or no
> longer supports the program. Does anyone know if someone is supporting it?
> Is there a good alternative anyone knows of for viewing and tweeking
> alignments?  Thanks- Dave

Try http://www.cris.com/~ketchup/genedoc.shtml for the multiple alignment
editor "GenDoc" - though its only for MSWindows so far.

Jeffrey Keen


From owner-software@net.bio.net Thu Feb 06 22:00:00 1997
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!www.nntp.primenet.com!nntp.primenet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!worldnet.att.net!cbgw2.lucent.com!news.bu.edu!usenet
From: James Freeman <jfreeman@darwin.bu.edu>
Newsgroups: bionet.software
Subject: Online Genomic Analysis Tool From The BMERC
Date: Fri, 07 Feb 1997 14:25:17 -0500
Organization: BioMolecular Engineering Resource Center
Lines: 53
Message-ID: <32FB819D.4487EB71@darwin.bu.edu>
NNTP-Posting-Host: hinshelwood.bu.edu
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.01Gold (X11; I; SunOS 4.1.4 sun4m)
CC: jfreeman@darwin.bu.edu

BMERC/MBCRR Bulletin:  February 1997
 
WWW interface for searching several Genomes at once: (NEW--Includes E.
Coli!)
 
The E. Coli genome has just been sequenced as of the last week of
January.
At BMERC, we have added the E. Coli genome to our genome blast page.
The E. Coli genome is of particular interests to Molecular Biologists
because the vast majority of functions assigned to E. Coli have been
done
by experimentation.  Most other genomes have their functions assigned by
homology.
 
The complete and nearly complete genomes of Saccharomyces
Cerevisiae , Methanococcus Jannaschii, E. Coli, and Bacillus Subtilis
are
now available. Our WWW Blast interface allows you to search,
using your sequence, against two subsets of the available putative
open reading frames of these genomes using blastp.
A Search Against Annotated ORF's and a Search Against Unannotated
ORF's of these genomes are the search options available from this page.
 
Your output will consist of detailed references for the significant
blast matches and the raw blast output. The detailed references consist
of a reference key, the annotation where available, the protein
sequence, and the dna for the ORF's.
 
We are also providing a tool for function keyword searching.We have
built a table that relates E. Coli to the other three main genomes
using blast with a Karlan Altschul score of < 10E-17.
This keyword searching tool will print a list of every sequence
identifier
that is close to the E. Coli gene of the cluster where the keyword is
found.
 
The WWW address for our Blast Genome Analysis blast page is:
 
http://bmerc-www.bu.edu/genome/genomeblastp.html

The WWW address for the E Coli functional search is:

http://bmerc-www.bu.edu/genome/ecoli-keyword.html

If you have any questions/comments on this WWW page, please
send them to Jim Freeman at jfreeman@darwin.bu.edu


-- 
Jim Freeman  P: mammon@tiac.net W: jfreeman@darwin.bu.edu
Programmer/Analyst at Bio-Molecular Engineering Center at BU
Etiam unum capillum umbram suam habet.
http://www.tiac.net/users/mammon/index.html

From owner-software@net.bio.net Fri Feb 07 22:00:00 1997
Path: biosci!agate!howland.erols.net!news-peer.gsl.net!news.gsl.net!news-hk.gsl.net!news.gsl.net!newsgate.cuhk.edu.hk!news.polyu.edu.hk!usenet
From: bcyeung@polyu.edu.hk (bcyeung)
Newsgroups: alt.hypertext,alt.windows95,alt.html,bionet.software
Subject: Re: ms word to html
Date: Sat, 08 Feb 1997 08:34:01 GMT
Organization: polyu.edu.hk
Lines: 19
Message-ID: <32fc3a07.10451493@hkpu01.polyu.edu.hk>
References: <855165652.25149@dejanews.com>
NNTP-Posting-Host: 158.132.16.169
X-Newsreader: Forte Free Agent 1.1/32.230

On Wed, 05 Feb 1997 12:11:06 -0600, sxr3008@megahertz.njit.edu wrote:

>Hi, there:
>
>I am trying to write my own word-to-html converter. But I don't know
>how the MS word's .doc file is formated. There must be some format
>specification like html's tags or DTD. But I have no idea where to find
>it out. Can anybody give some hints?
>
>TIA.
>
>Shaomei
>
>-------------------==== Posted via Deja News ====-----------------------
>      http://www.dejanews.com/     Search, Read, Post to Usenet

Windows 95 have the internet assistant for change word doccument file
to html file automatically , try to download the freeware from
http://www.microsof.com

From owner-software@net.bio.net Fri Feb 07 22:00:00 1997
Path: biosci!agate!spool.mu.edu!uwm.edu!newsfeeds.sol.net!nntp.uio.no!Norway.EU.net!sn.no!newsfeed.tip.net!puff.lmv.lm.se!newsfeed3.telia.com!masternews.telia.net!newssrv.ita.tip.net!mikasa.iol.it!news
From: edoardo.shockwav@iol.it (Edo)
Newsgroups: bionet.software
Subject: male and female symbol
Date: Fri, 07 Feb 1997 20:56:18 GMT
Organization: Italia Online
Lines: 34
Message-ID: <32fc8e3a.2129086@news.iol.it>
NNTP-Posting-Host: 194.20.26.68
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Newsreader: Forte Agent .99g/32.339

I am looking for a TTF (True Type Font, for windows) that includes the
male and female symbols.
Is there anyone that could help me?

Edo


              o o o o
                  o o
                o   o
              o     o
       oooo o
     o      o
   o          o
  o            o
   o          o
     o      o
       oooo


       ooo 
     o     o
   o         o
  o           o
   o         o
     o     o
       ooo
        o
        o
      ooooo
        o
        o



From owner-software@net.bio.net Fri Feb 07 22:00:00 1997
Path: biosci!agate!howland.erols.net!torn!kwon!hone!informer1.cis.McMaster.CA!roger.interlynx.net!usenet
From: Lars Thomsen <lthomsen@interlynx.net>
Newsgroups: bionet.software
Subject: Re: What is your favorite patch-clamp hardware/software?
Date: Sat, 08 Feb 1997 05:34:13 -0800
Organization: Interlynx
Lines: 278
Message-ID: <32FC80D3.3EC1@interlynx.net>
References: <32f99325.3279390@news.uni-marburg.de>
NNTP-Posting-Host: ppp05-max1.interlynx.net
Mime-Version: 1.0
Content-Type: multipart/mixed; boundary="----------9B1215B2B560"
X-Sender: Lars Thomsen <lthomsen@interlynx.net> (Unverified)
X-Mailer: Mozilla 4.0b1 (Win95; I)
To: Hans-Georg Klieber <klieber@mailer.uni-marburg.de>
X-Priority: Normal

This is a multi-part message in MIME format.
------------9B1215B2B560
Content-Type: multipart/alternative; boundary="----------4BBA602038701"


------------4BBA602038701
Content-Transfer-Encoding: 7bit
Content-Type: text/plain; charset=us-ascii

Dear Hans Georg Klieber
I have used several software packages for my patch clamp data
acquisition and analysis. Here are my comments which also covers
customer support from the companies providing the software.

Axon Instruments is providing a software package for DOS called PClamp6
and it is well supported with a thick and comprehensive manual and they
have a good tech support that answers the phone or your email. The
program is getting outdated. It suffers from the limitations in DOS and
a menu structure that is diffuse so that you will find e.g. hardware
settings in many different submenus instead of just one. I know that
they are coming out with a new version for Win95 (maybe also Win31) and
that could help a lot. The problem with data acquisition programs under
Win95 or Win31 is that the screen update is normally slow and "jumpy".
However, if they have used the new technology embedded in DirectX from
Microsoft then they might have aschieved something useful, but I want to
see it before I buy it. Pclamp6 is divided into two applications for
data acquisition (FetchEx and ClampEx). FetcEx which is used for
recording gapfree data (contineous recording) does not support gapfree
data and I sometimes use the program called Axotape instead. I could say
more bad things but infact PClamp6 is doing the job and if you enable
the telegraphed output then the program automatically reads the gain
from your amplifier so that you dont have to scripple it down in your
logbook.

That is a neat feature that I should be normal but few hardware
companies support it.  I use Axon Instruments Axopatch 200A and they
have now made a 200b version with a cooled headstage that gives you
extremely lownoise recording if you wants to do single channel work. The
Axon 200A is without doubt the best I have tried. I wish they were as
good programmers as they are hardware designers. I use the
dataacquisition that was sitting on my setup when I arrived in the lab
it is called TL-1 or something and I have no good feelings about that.
The new Digidata looks much better.


Cambrigde Electronic Design UK has a package called Patch and Voltage
Clamp 6 and it is very similar to the Pclamp6. The manual is horrible
but the support is really good from CED and if you find a bug then you
can get a fix in no time. I dont like the Patch and Voltage Clamp
software from CED it is a clumsy program but they have another package
that is really good. It is called Spike2 and it is their major software
and Axon doesn't really match it with any of their current products. I
had to do some pharmacological experiments and Spike2 gave me everything
that I needed. The best thing is that you can record several channels at
different speed. That is not possible with anything from Axon. The power
of this is that if you e.g. have a temperature probe sitting in your
setup then you can record from that at e.g. 100Hz while you record you
patch data at 10KHz...see that is smart. Another feature that simply is
brilliant for the working electrophysiologist ist the ability to disable
recording for periods of times - just by a click on a button. Imagine
that you have to do a dose-response curve, beween each application of
the agonist you have to wait e.g. 5 minutes, instead of recording the
space between the "active periods" then you simply disable writing to
disc. The program still keeps track of time. That is possible via the
1401plus which is the data acquisition unit from CED. It is quite
expensive but it is worth the money. A look at Axons newest Digidata
convinced me that they had copied the look of the 1401plus, but I doubt
they also have copied the inside of the machine. All my regards to the
CED team. The new spike2 for windows (including NT and Win95) gives you
the options for customizing your program. You can build scripts into it.
I made an article about the DOS version (Thomsen et al Journal of
Neuroscience Methods 65, 19-26 1996).

Other analysis programs.
If you have a Mac then you are lucky because then you can get Axograph
which is a brilliant program that has all that Pclamp6 is missing. Armed
with Pclamp6 and Axograph you can do a lot.

I know that another software company called Bruxton Corporation
(formerly known as SKALAR Instruments)  they have also made a quite
impressive application and that is for the PC.

Other data acquisition programs
If I was going to buy something new just to increase my experience then
I would take a good look a the new cards from National Instruments and
some of the many dataacquisiton packages they make. The problem is that
whatever dedicated package you buy you will always meet the limitations
faster than you thought you would. Therefore it is a must to have access
to a programming language that is easy and bugfree. National Instruments
supports Visual basic and I dont think that you can find anything easier
than that. VB5 is coming out in marts 97 and it will give you impressive
performance compared to VB4 (upto 2000 % increase in e.g. form loading)

Finally I will mention my own program which is freeware. It reads my
datafiles created with Pclamp6 and somebody in the lab uses PClamp52 so
I made it read that format too.

What I would do :
Pharmacological experiments - buy the Axon 200 A, the 1401plus and
Spike2 for Windows from Cambrigde Electronic Design

Whole cell recording - buy the Axon 200A, Digidata and Pclamp6 and use
ClampEx.

Single channel recording - good luck

Best Regards
--
Lars Thomsen, MSc. PhD
115 South Oval, Hamilton, L8S1R2 Ontario Canada
email lthomsen@interlynx.net
homepage http://home.interlynx.net/~lthomsen/index.htm
tlp 1 905 777 0720  fax 1 905 777 0738


------------4BBA602038701
Content-Transfer-Encoding: 7bit
Content-Type: text/html; charset=us-ascii

<HTML><BODY>

<DT>Dear Hans Georg Klieber</DT>

<DT>I have used several software packages for my patch clamp data acquisition
and analysis. Here are my comments which also covers customer support from
the companies providing the software.&nbsp;</DT>

<DT>&nbsp;</DT>

<DT>Axon Instruments is providing a software package for DOS called PClamp6
and it is well supported with a thick and comprehensive manual and they
have a good tech support that answers the phone or your email. The program
is getting outdated. It suffers from the limitations in DOS and a menu
structure that is diffuse so that you will find e.g. hardware settings
in many different submenus instead of just one. I know that they are coming
out with a new version for Win95 (maybe also Win31) and that could help
a lot. The problem with data acquisition programs under Win95 or Win31
is that the screen update is normally slow and &quot;jumpy&quot;. However,
if they have used the new technology embedded in DirectX from Microsoft
then they might have aschieved something useful, but I want to see it before
I buy it. Pclamp6 is divided into two applications for data acquisition
(FetchEx and ClampEx). FetcEx which is used for recording gapfree data
(contineous recording) does not support gapfree data and I sometimes use
the program called Axotape instead. I&nbsp;could say more bad things but
infact PClamp6 is doing the job and if you enable the telegraphed output
then the program automatically reads the gain from your amplifier so that
you dont have to scripple it down in your logbook.&nbsp;</DT>

<DT>&nbsp;</DT>

<DT>That is a neat feature that I should be normal but few hardware companies
support it.&nbsp; I&nbsp;use Axon Instruments Axopatch 200A and they have
now made a 200b version with a cooled headstage that gives you extremely
lownoise recording if you wants to do single channel work. The Axon 200A
is without doubt the best I have tried. I wish they were as good programmers
as they are hardware designers. I&nbsp;use the dataacquisition that was
sitting on my setup when I arrived in the lab it is called TL-1 or something
and I have no good feelings about that. The new Digidata looks much better.&nbsp;</DT>

<DT>&nbsp;</DT>

<DT>&nbsp;</DT>

<DT>Cambrigde Electronic Design UK&nbsp;has a package called Patch and
Voltage Clamp 6 and it is very similar to the Pclamp6. The manual is horrible
but the support is really good from CED and if you find a bug then you
can get a fix in no time. I dont like the Patch and Voltage Clamp software
from CED it is a clumsy program but they have another package that is really
good. It is called Spike2 and it is their major software and Axon doesn't
really match it with any of their current products. I had to do some pharmacological
experiments and Spike2 gave me everything that I needed. The best thing
is that you can record several channels at different speed. That is not
possible with anything from Axon. The power of this is that if you e.g.
have a temperature probe sitting in your setup then you can record from
that at e.g. 100Hz while you record you patch data at 10KHz...see that
is smart. Another feature that simply is brilliant for the working electrophysiologist
ist the ability to disable recording for periods of times - just by a click
on a button.&nbsp;Imagine that you have to do a dose-response curve, beween
each application of the agonist you have to wait e.g. 5 minutes, instead
of recording the space between the &quot;active periods&quot; then you
simply disable writing to disc. The program still keeps track of time.
That is possible via the 1401plus which is the data acquisition unit from
CED. It is quite expensive but it is worth the money. A look at Axons newest
Digidata convinced me that they had copied the look of the 1401plus, but
I doubt they also have copied the inside of the machine. All my regards
to the CED team. The new spike2 for windows (including NT and Win95) gives
you the options for customizing your program. You can build scripts into
it. I made an article about the DOS&nbsp;version (Thomsen et al Journal
of Neuroscience Methods 65, 19-26 1996).</DT>

<DT>&nbsp;</DT>

<DT>Other analysis programs.</DT>

<DT>If you have a Mac then you are lucky because then you can get Axograph
which is a brilliant program that has all that Pclamp6 is missing. Armed
with Pclamp6 and Axograph you can do a lot.</DT>

<DT>&nbsp;</DT>

<DT>I know that another software company called Bruxton Corporation (formerly
known as SKALAR Instruments)&nbsp; they have also made a quite impressive
application and that is for the PC.</DT>

<DT>&nbsp;</DT>

<DT>Other data acquisition programs</DT>

<DT>If I was going to buy something new just to increase my experience
then I would take a good look a the new cards from National Instruments
and some of the many dataacquisiton packages they make. The problem is
that whatever dedicated package you buy you will always meet the limitations
faster than you thought you would. Therefore it is a must to have access
to a programming language that is easy and bugfree. National Instruments
supports Visual basic and I dont think that you can find anything easier
than that. VB5 is coming out in marts 97 and it will give you impressive
performance compared to VB4 (upto 2000 % increase in e.g. form loading)</DT>

<DT>&nbsp;</DT>

<DT>Finally I will mention my own program which is freeware. It reads my
datafiles created with Pclamp6 and somebody in the lab uses PClamp52 so
I made it read that format too.&nbsp;</DT>

<DT>&nbsp;</DT>

<DT>What I would do :</DT>

<DT>Pharmacological experiments - buy the Axon 200 A, the 1401plus and
Spike2 for Windows from Cambrigde Electronic Design</DT>

<DT>&nbsp;</DT>

<DT>Whole cell recording - buy the Axon 200A, Digidata and Pclamp6 and
use ClampEx.</DT>

<DT>&nbsp;</DT>

<DT>Single channel recording - good luck&nbsp;</DT>

<DT>&nbsp;</DT>

<DT>Best Regards</DT>

<DT>--&nbsp;<BR>
Lars Thomsen, MSc. PhD<BR>
115 South Oval, Hamilton, L8S1R2 Ontario Canada<BR>
email lthomsen@interlynx.net<BR>
homepage http://home.interlynx.net/~lthomsen/index.htm<BR>
tlp 1 905 777 0720&nbsp; fax 1 905 777 0738<BR>
&nbsp;</DT>

</BODY>
</HTML>
------------4BBA602038701--

------------9B1215B2B560
Content-Transfer-Encoding: 7bit
Content-Description: Address Book Card for Lars Thomsen
Content-Disposition: inline; filename="nsmailBQ.TMP"
Content-Type: text/x-vCard; charset=us-ascii; name="nsmailBQ.TMP"

BEGIN:VCARD
FN:Lars Thomsen
N:Thomsen;Lars
ORG:McMaster University
ADR:;;115 South Oval;Hamilton;Ontario;L8S1R2
EMAIL;INTERNET:lthomsen@interlynx.net
TITLE:MSc PhD
TEL;WORK:1 905 5259140 x22584
TEL;FAX:1 905 7770720
TEL;HOME:1 905 7770738
X-NAV-HTML:T
END:VCARD


------------9B1215B2B560--


From owner-software@net.bio.net Fri Feb 07 22:00:00 1997
Path: biosci!rutgers.rutgers.edu!uwm.edu!news-peer.gsl.net!news.gsl.net!news.sprintlink.net!news-peer.sprintlink.net!howland.erols.net!news.nacamar.de!fu-berlin.de!news.th-darmstadt.de!news.uni-mainz.de!usenet
From: "Dr. K. Eimert" <eimert@geisenheim.fa.fh-wiesbaden.de>
Newsgroups: bionet.software
Subject: Re: Windows: sequence analysis
Date: Wed, 05 Feb 1997 12:17:00 +0100
Organization: Forschungsanstalt Geisenheim
Lines: 18
Message-ID: <32F86C2C.8AE@geisenheim.fa.fh-wiesbaden.de>
References: <5cpibd$jnl$1@mark.ucdavis.edu> <32F7948C.79E@molbiol.uct.ac.za>
Reply-To: eimert@geisenheim.fa.fh-wiesbaden.de
NNTP-Posting-Host: 194.94.6.60
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.0 (Win95; I)

Hi Ed,

that sounds really good. Unfortunately, I couldn' find any info on this
company (or program). Could you post their address, please? Do they have
a WEB site or a demo or sw version available on some ftp sites (my
searches didn't turn out anything, though)?
Thanx in advance,

Klaus

------------------------------------------------------------------------
Dr. Klaus Eimert
Dept. of Botany
State Research Institute Geisenheim
Von-Lade-Str. 1                                 Tel.: + 49 6722 502 469
D-65366 Geisenheim                              FAX:  + 49 6722 502 460
Germany                    e-mail: eimert@geisenheim.fa.fh-wiesbaden.de
------------------------------------------------------------------------

From owner-software@net.bio.net Fri Feb 07 22:00:00 1997
Path: biosci!daresbury!nntp-trd.UNINETT.no!sn.no!nntp.uio.no!news.apfel.de!fu-berlin.de!news.th-darmstadt.de!news.uni-mainz.de!usenet
From: "Dr. K. Eimert" <eimert@geisenheim.fa.fh-wiesbaden.de>
Newsgroups: bionet.software
Subject: Q: inducible promoters for transgenics?
Date: Thu, 06 Feb 1997 08:18:31 +0100
Organization: Forschungsanstalt Geisenheim
Lines: 23
Message-ID: <32F985C7.3B28@geisenheim.fa.fh-wiesbaden.de>
Reply-To: eimert@geisenheim.fa.fh-wiesbaden.de
NNTP-Posting-Host: 194.94.6.60
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.0 (Win95; I)

Dear Netters,

I am in need of an inducible promoter for plant transformation. It is
important that this promoter would not "leak" if not induced, e.g., the
hs-promoter would not work for us. Does anybody have experience with the
glucocorticoid-inducible promoter? 
I seem to remember a paper some three (or more) years ago dealing with a
Cu-inducible promoter. Did anybody try this one? Are the inducing
concentrations of Cu toxic for the plants? 
Is there any review dealing with this topic, by chance?
I'd appreciate any help or comments! 
Thanx in advance,

Klaus

------------------------------------------------------------------------
Dr. Klaus Eimert
Dept. of Botany
State Research Institute Geisenheim
Von-Lade-Str. 1                                 Tel.: + 49 6722 502 469
D-65366 Geisenheim                              FAX:  + 49 6722 502 460
Germany                    e-mail: eimert@geisenheim.fa.fh-wiesbaden.de
------------------------------------------------------------------------

From owner-software@net.bio.net Fri Feb 07 22:00:00 1997
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.inet.co.th!nectec!news.mahidol.ac.th!mucc.mahidol.ac.th!u3601087
From: Panapat Uawithya <u3601087@mahidol.ac.th>
Newsgroups: bionet.software
Subject: need help in PDB size measure
Date: Sat, 8 Feb 1997 20:32:04 +0700
Organization: Mahidol University, Thailand
Lines: 16
Message-ID: <Pine.OSF.3.95.970208202927.13944B-100000@mucc.mahidol.ac.th>
NNTP-Posting-Host: 202.28.162.1
Mime-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII

 Hello,
  I want to size an dangle of each secondary structure in PDB . I try
measure using kinemage but it's very difficult and borsum .Are there any
program that can measure it more accurate and easy.

 """"""" *********************************************************     
 | - - |  Name Panapat Uawithya         
(|[O O]|) Nickname Nuk    
 |  \  |  Address 13 soi 13 Saeree vlg1 Huamark Bangkapi     
 (  `' )          Bangkok Thailand 10250
  \ ~ /   Tel 662-319-4925
   \ /    E-mail address u3601087@mucc.mahidol.ac.th
   /*\    Homepage www.mahidol.ac.th/~u3601087
******************************************************************************
  


From owner-software@net.bio.net Fri Feb 07 22:00:00 1997
Newsgroups: bionet.software,bionet.molbio.methds-reagnts
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!torn!utnut!oci!kinase.oci.utoronto.ca
From: jwoodget@oci.utoronto.ca (Jim Woodgett)
Subject: Re: Restriction Enzymes in DNA Strider?
Content-Type: text/plain; charset=US-ASCII
Message-ID: <9702070934.AA22100@kinase.oci.utoronto.ca>
Sender: news@oci.utoronto.ca
Reply-To: jwoodget@oci.utoronto.ca (Jim Woodgett)
Organization: Ontario Cancer Institute
X-Newsreader: InterCon tcpCONNECT4 4.0.3d03
References: <mantei-0602970937530001@retrograde.ethz.ch>
Mime-Version: 1.0
Date: Fri, 7 Feb 1997 14:34:22 GMT
Lines: 41
Xref: biosci bionet.software:17830 bionet.molbio.methds-reagnts:54564

In article <mantei-0602970937530001@retrograde.ethz.ch>, 
mantei@neuro.biol.ethz.ch (Ned Mantei) writes:

> This is a reply message which refers to the following message: News:
> <nc1-0402971130080001@phanes94.mc.duke.edu>   
> In article <nc1-0402971130080001@phanes94.mc.duke.edu>, nc1@
> acpub.duke.edu (Namjin Chung) wrote: 
  
> > Dear DNA Strider Users: 
> > I use the above software for some sequence processing, and I think it's 
> > simple but very good software.  The only one thing I'm not satisfied 
> > with it is that it would not allow custom restriction by a selected set 
> > of REs. 
  
> If you hold down the option key while choosing "Graphic map..." from the 
> menu "Enz", you can choose from the restriction enzymes in the file 
> RELibrary.RELibrary can be edited by a word processor (save as "Text 
> only"). Or is this not what you meant? 

The Option key also works with the "Digestion..." menu choice.  When the list 
of enzymes comes up (its a separate window) you can select as many enzymes as 
you want to cut with by holding the shift key down.  To deselect all, click on 
the space at the upper left of the listing.  You can also combine particular 
cutters with double, triple cutters etc with a button selection in the dialog 
box.

Once you've chosen a selection of REs and displayed the map or digest, any 
subsequent maps/digests of other sequences will also use the same selection 
unless you reselect the REs or quit/restart the application.

All this is in the manual.

Jim Woodgett


Associate Professor: University of Toronto
Senior Scientist: Ontario Cancer Institute   mailto:jwoodget@oci.utoronto.ca
610 University Avenue         http://kinase.oci.utoronto.ca/woodgettlab.html
Toronto, Ontario M5G 2M9           phone (416) 946-2962  fax: (416) 946-2963



From owner-software@net.bio.net Fri Feb 07 22:00:00 1997
Path: biosci!genesys-tech.com!jgolden
From: jgolden@genesys-tech.com (jim golden)
Newsgroups: bionet.software
Subject: job opportunity in Madison, WI - update
Date: 8 Feb 1997 08:43:42 -0800
Organization: genesys technologies
Lines: 12
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <32FCABB4.7FDF@genesys-tech.com>
NNTP-Posting-Host: net.bio.net

Thanks to all that have sent me resumes regarding the software/bioinformatics position at GeneSys Technologies, Inc.  Unfortunately, because 
of the large volume of responses I received, I don't have time to answer everyone individually.  The position was filled this week, but I will 
keep all the resumes I received on file since we are expecting rapid expansion this year in the areas of robotics and image processing.  The 
quality of the applicants has been very high, and I encourage those looking for a position in bioinformatics to check out the 17 January 1997 
issue of the journal Science for several want ads in this area.  There a many research-oriented firms recruiting for projects in sequencing, 
gene finding, and analysis.  Thanks again to all who responded.

Jim Golden
Director of Bioinformatics
GeneSys Technologies, Inc.
9757 Wilkinson Rd.
Mazomanie, WI 53560

From owner-software@net.bio.net Sat Feb 08 22:00:00 1997
Path: biosci!biosci!not-for-mail
From: Rong-i Hong <rong@bioch.ox.ac.uk>
Newsgroups: bionet.molbio.proteins,bionet.xtallography,bionet.software,bionet.molec-model,bionet.biophysics,sci.chem
Subject: Protein 2nd structure assignment
Date: 9 Feb 1997 14:00:30 -0800
Organization: Oxford University
Lines: 18
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <32FB0643.41C6@bioch.ox.ac.uk>
References: <32E977EB.5290@plaza.snu.ac.kr> <32F0B455.43D4@chem.gla.ac.uk>
NNTP-Posting-Host: net.bio.net
Xref: biosci bionet.molbio.proteins:9946 bionet.xtallography:3183 bionet.software:17841 bionet.molec-model:1364 bionet.biophysics:2621 sci.chem:75051

Dear All,

We are doing some work about assignments of protein secondary sturcture
elements. Are there any programs, other than DSSP, DEFINE and STRIDE, to
define secondary structure based on the atomic coordinates? 
If so, could you let me know where I might acquire such programs?

Thanks in advance.

------------------------------------------------------------
Rong-I Hong
Lab. of Molecular Biophysics      EMail: rong@bioch.ox.ac.uk
University of Oxford              Tel: 01865-275 369
Rex Richards Building             Fax: 01865-275 182
Oxford, OX1 3QU
United Kingdom
------------------------------------------------------------


From owner-software@net.bio.net Sat Feb 08 22:00:00 1997
Path: biosci!agate!spool.mu.edu!uwm.edu!cs.utexas.edu!howland.erols.net!torn!hone!informer1.cis.McMaster.CA!roger.interlynx.net!usenet
From: Lars Thomsen <lthomsen@interlynx.net>
Newsgroups: bionet.software
Subject: Re: What is your favorite patch-clamp hardware/software?
Date: Sat, 08 Feb 1997 20:04:33 -0800
Organization: Interlynx
Lines: 118
Message-ID: <32FD4CD1.4D86@interlynx.net>
References: <32f99325.3279390@news.uni-marburg.de> <32FC80D3.3EC1@interlynx.net>
NNTP-Posting-Host: ppp43-cas1.interlynx.net
Mime-Version: 1.0
Content-Type: multipart/mixed; boundary="----------790956C2599B4"
X-Sender: Lars Thomsen <lthomsen@interlynx.net> (Unverified)
X-Mailer: Mozilla 4.0b1 (Win95; I)
To: Lars Thomsen <lthomsen@interlynx.net>
X-Priority: Normal

This is a multi-part message in MIME format.
------------790956C2599B4
Content-Type: multipart/alternative; boundary="----------63615737B2F5"


------------63615737B2F5
Content-Transfer-Encoding: 7bit
Content-Type: text/plain; charset=us-ascii

In my previous answer I wrote :
"FetctEx which is used for recording gapfree data (contineous recording)
does not support gapfree data and I sometimes use the program called
Axotape instead"
This should have been :
FetcEx which is used for recording gapfree data (contineous recording)
does not support "tags" and I sometimes use the program called Axotape
instead. Tags are small time events stored in the end of the file. They
are nice when you are doing something to your cell e.g. adding a agonist
and then you make a time tag so you can keep track of what is happening.

more about tags:
I have only seen a advanced tagging system from Biologic and their
computer controlled solution changer. The software was made so that you
could call up solutions from a database and use them as tags. More or
less what I remember. However, it should be standard in all
electrophysiological acquisition programs that you can call up a
database for the tagging. For highspeed recording you could set the tags
that you use before. New data acquisition systems are build in a modular
way so that you use two computers instead of one. The acquistion
computer is dedicated to the timing and sampling and has a lot of
memory, the PC is used as the control interface and allows you to do a
lot of things that not was possible in the old days where you could ruin
the timing of the sampling. The dual computer system gives you a buffer
effect that allows you to do some sort of multitasking. A good example
is the 1401plus from Cambridge Electronic Design.

--
Lars Thomsen, MSc. PhD
115 South Oval, Hamilton, L8S1R2 Ontario Canada
email lthomsen@interlynx.net
homepage http://home.interlynx.net/~lthomsen/index.htm
tlp 1 905 777 0720  fax 1 905 777 0738


------------63615737B2F5
Content-Transfer-Encoding: 7bit
Content-Type: text/html; charset=us-ascii

<HTML><BODY>

<DT>In my previous answer I wrote :</DT>

<DT>&quot;FetctEx which is used for recording gapfree data (contineous
recording) does not support gapfree data and I sometimes use the program
called Axotape instead&quot;</DT>

<DT>This should have been :</DT>

<DT>FetcEx which is used for recording gapfree data (contineous recording)
does not support &quot;tags&quot; and I sometimes use the program called
Axotape instead. Tags are small time events stored in the end of the file.&nbsp;They
are nice when you are doing something to your cell e.g. adding a agonist
and then you make a time tag so you can keep track of what is happening.</DT>

<DT>&nbsp;</DT>

<DT>more about tags:</DT>

<DT>I have only seen a advanced tagging system from Biologic and their
computer controlled solution changer. The software was made so that you
could call up solutions from a database and use them as tags. More or less
what I remember. However, it should be standard in all electrophysiological
acquisition programs that you can call up a database for the tagging. For
highspeed recording you could set the tags that you use before. New data
acquisition systems are build in a modular way so that you use two computers
instead of one. The acquistion computer is dedicated to the timing and
sampling and has a lot of memory, the PC is used as the control interface
and allows you to do a lot of things that not was possible in the old days
where you could ruin the timing of the sampling. The dual computer system
gives you a buffer effect that allows you to do some sort of multitasking.
A good example is the 1401plus from Cambridge Electronic Design.</DT>

<DT>&nbsp;</DT>

<DT>--&nbsp;<BR>
Lars Thomsen, MSc. PhD<BR>
115 South Oval, Hamilton, L8S1R2 Ontario Canada<BR>
email lthomsen@interlynx.net<BR>
homepage http://home.interlynx.net/~lthomsen/index.htm<BR>
tlp 1 905 777 0720&nbsp; fax 1 905 777 0738<BR>
&nbsp;</DT>

</BODY>
</HTML>
------------63615737B2F5--

------------790956C2599B4
Content-Transfer-Encoding: 7bit
Content-Description: Address Book Card for Lars Thomsen
Content-Disposition: inline; filename="nsmailJC.TMP"
Content-Type: text/x-vCard; charset=us-ascii; name="nsmailJC.TMP"

BEGIN:VCARD
FN:Lars Thomsen
N:Thomsen;Lars
ORG:McMaster University
ADR:;;115 South Oval;Hamilton;Ontario;L8S1R2
EMAIL;INTERNET:lthomsen@interlynx.net
TITLE:MSc PhD
TEL;WORK:1 905 5259140 x22584
TEL;FAX:1 905 7770720
TEL;HOME:1 905 7770738
X-NAV-HTML:T
END:VCARD


------------790956C2599B4--


From owner-software@net.bio.net Sat Feb 08 22:00:00 1997
Path: biosci!rutgers.rutgers.edu!uwm.edu!newsfeeds.sol.net!nntp.uio.no!news.apfel.de!news-fra1.dfn.de!news-koe1.dfn.de!main.Germany.EU.net!Dortmund.Germany.EU.net!gsf.de!news
From: Burkhard Morgenstern <morgenst@gsf.de>
Newsgroups: bionet.software
Subject: New alignment program DIALIGN
Date: Fri, 07 Feb 1997 12:53:14 +0100
Organization: GSF Forschungszentrum fuer Umwelt und Gesundheit mbH
Lines: 48
Message-ID: <32FB17AA.41C6@gsf.de>
NNTP-Posting-Host: ariane.gsf.de
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.0Gold (X11; I; OSF1 V3.2 alpha)

We would like to announce the availability of
an alignment program called

                  DIALIGN
                  *******

DIALIGN is a completely novel program for pairwise
as well as multiple alignment of nucleic acid and
protein sequences. It is especially suited to detect
local similarities in otherwise completely unrelated
sequences and it does not employ any kind of gap penalty.



The method is described in

  B. Morgenstern, A. Dress, T. Werner:
  Multiple DNA and protein sequence alignment based on
  segment-to-segment comparison
  Proc. Natl. Acad. Sci. 93 pp. 12098 - 12103 (1996)

DIALIGN executables for various UNIX platforms (DEC,
SGI, CONVEX, HP, SUN) together with a detailed user
guide, online help and a test example are available at

  http://www.gsf.de/biodv/dialign.html

or via ftp:

  ftp://ariane.gsf.de/pub/unix/dialign

More detailed information on DIALIGN is available at
our web page. If you have any questions, please feel
free to contact us by e-mail.

  Burkhard Morgenstern


----------------------------------------------------------

Dr. Burkhard Morgenstern
GSF - National Research Center for Environment and Health,
Institute of Biomathematics and Biometry
Ingolstaedter Landstr. 1,
85764 Neuherberg,
Germany

morgenstern@gsf.de

From owner-software@net.bio.net Sat Feb 08 22:00:00 1997
Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!rill.news.pipex.net!pipex!tank.news.pipex.net!pipex!duke.telepac.pt!news.telepac.pt!usenet
From: nop53686@mail.telepac.pt (Miguel Garcia)
Newsgroups: bionet.software
Subject: I/O PROGRAM
Date: Sun, 09 Feb 1997 19:30:22 GMT
Lines: 9
Message-ID: <32fe2113.453525@news.telepac.pt>
NNTP-Posting-Host: lc1_p6.telepac.pt
X-Newsreader: Forte Free Agent 1.1/32.230

Does anyone knows if there is at anywhere an oriented objects software
(or simply windows based) for free to manipulate I/O PC cards?

	If yes, please contact me.

	Thanks
Miguel Garcia
Técnico Electrónica (Desenvolvimento Projectos Automação)
e-mail:nop53686@mail.telepac.pt

From owner-software@net.bio.net Sat Feb 08 22:00:00 1997
Path: biosci!agate!spool.mu.edu!uwm.edu!www.nntp.primenet.com!nntp.primenet.com!howland.erols.net!swrinde!ihnp4.ucsd.edu!munnari.OZ.AU!news.mel.connect.com.au!news.mel.aone.net.au!news.nlc.net.au!news
From: "Cameron Johnson" <cameron@nlc.net.au>
Newsgroups: bionet.software
Subject: Great Software deals
Date: 9 Feb 1997 04:39:33 GMT
Organization: NHJ NORTHLINK COMMUNICATIONS
Lines: 7
Distribution: inet
Message-ID: <01bc1643$5fc1fd60$618518cb@default>
NNTP-Posting-Host: dialine24.nlc.net.au
Mime-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Content-Transfer-Encoding: 7bit
X-Newsreader: Microsoft Internet News 4.70.1155

To Everyone 
If anyone is looking for Software at low Prices then Go to 
http://www.nlc.net.au/~cameron/software.html
After finding the software you want email me and in the subject box type
I want Whatever software title you want.
Cameron Johnson


From owner-software@net.bio.net Sat Feb 08 22:00:00 1997
Path: biosci!agate!howland.erols.net!torn!hone!informer1.cis.McMaster.CA!roger.interlynx.net!usenet
From: Lars Thomsen <lthomsen@interlynx.net>
Newsgroups: bionet.software
Subject: Re: What is your favorite patch-clamp hardware/software?
Date: Sat, 08 Feb 1997 20:52:09 -0800
Organization: Interlynx
Lines: 140
Message-ID: <32FD57F9.470F@interlynx.net>
References: <32f99325.3279390@news.uni-marburg.de>
NNTP-Posting-Host: ppp43-cas1.interlynx.net
Mime-Version: 1.0
Content-Type: multipart/mixed; boundary="----------4D3F2E2B38B46"
X-Sender: Lars Thomsen <lthomsen@interlynx.net> (Unverified)
X-Mailer: Mozilla 4.0b1 (Win95; I)
To: Hans-Georg Klieber <klieber@mailer.uni-marburg.de>
X-Priority: Normal

This is a multi-part message in MIME format.
------------4D3F2E2B38B46
Content-Type: multipart/alternative; boundary="----------18B62044158A7"


------------18B62044158A7
Content-Transfer-Encoding: 7bit
Content-Type: text/plain; charset=us-ascii

More about controlling an experiment :

The TTL ports of the dataacquisition system can be used to control your
setup. If you system can record that a TTL output has be fired then it
is smart, because then you have a way to store an event marker in your
data trace. If your system allows you to record TTL in but not TTL out
commands then you can hook your TTL output to a TTL input and then
record that - its a little bit primitive but it works and you get your
eventmarker. The events are normally stored in separate event channels
which only contain a very limited information even when sampled at high
frequencies, the reason is that only the time location is stored, maybe
with a comment attached to it. The event markers are unvaluable when
making the analysis of continuous data. A simple searchroutine finding
the next event in an event channel can take you to the next interesting
point in the datafile. If you have a good event marker system then you
can record all your data in one file rather than splitting it up in
fractions. Right now I am using PClamp6 and there is no way to store
events so I suffer under the load of many many datafiles, which is
needed to keep track of the flow of the experiment. When you have your
events then it is a piece of cake of doing analysis even with huge
datafiles. I recorded 6.5 GB of data from enteric neurones during my PhD
and without the eventmarkes my analysis would have been a nighmare.  At
present momement I am trying to find a way to change my solutions with a
little robot controlled by TTL pulses from my computer. I have tried
several systems but right now I think I am on the track of something
good. The beauty is that if you can store these events together with all
other kinds of events e.g. focal stimulation or whatever..
I cant help it but I have never seen anything as smart as the system
from Cambrigde Electronic Design. I would wish they would make some
scripts for Spike2 so that it would run whole cell experiments, I made
some but it is always hard because it takes time to develop software and
the experiments are after all the most important. The smart thing about
Spike2 is that it is one program that can be customized to do anything.
A feature that I would like is that if you e.g. do a lot of different
files during one recording then you can save it all as a "project"
simililar to Microcal Origin 4.

Best Regards
Lars Thomsen
--
Lars Thomsen, MSc. PhD
115 South Oval, Hamilton, L8S1R2 Ontario Canada
email lthomsen@interlynx.net
homepage http://home.interlynx.net/~lthomsen/index.htm
tlp 1 905 777 0720  fax 1 905 777 0738


------------18B62044158A7
Content-Transfer-Encoding: 7bit
Content-Type: text/html; charset=us-ascii

<HTML><BODY>

<DT>More about controlling an experiment :</DT>

<DT>&nbsp;</DT>

<DT>The TTL&nbsp;ports of the dataacquisition system can be used to control
your setup. If you system can record that a TTL&nbsp;output has be fired
then it is smart, because then you have a way to store an event marker
in your data trace. If your system allows you to record TTL&nbsp;in but
not TTL&nbsp;out commands then you can hook your TTL&nbsp;output to a TTL
input and then record that - its a little bit primitive but it works and
you get your eventmarker. The events are normally stored in separate event
channels which only contain a very limited information even when sampled
at high frequencies, the reason is that only the time location is stored,
maybe with a comment attached to it. The event markers are unvaluable when
making the analysis of continuous data. A simple searchroutine finding
the next event in an event channel can take you to the next interesting
point in the datafile. If you have a good event marker system then you
can record all your data in one file rather than splitting it up in fractions.
Right now I am using PClamp6 and there is no way to store events so I suffer
under the load of many many datafiles, which is needed to keep track of
the flow of the experiment. When you have your events then it is a piece
of cake of doing analysis even with huge datafiles. I recorded 6.5 GB of
data from enteric neurones during my PhD and without the eventmarkes my
analysis would have been a nighmare.&nbsp; At present momement I am trying
to find a way to change my solutions with a little robot controlled by
TTL&nbsp;pulses from my computer. I have tried several systems but right
now I think I am on the track of something good. The beauty is that if
you can store these events together with all other kinds of events e.g.
focal stimulation or whatever..</DT>

<DT>I cant help it but I have never seen anything as smart as the system
from Cambrigde Electronic Design. I would wish they would make some scripts
for Spike2 so that it would run whole cell experiments, I made some but
it is always hard because it takes time to develop software and the experiments
are after all the most important. The smart thing about Spike2 is that
it is one program that can be customized to do anything. A feature that
I would like is that if you e.g. do a lot of different files during one
recording then you can save it all as a &quot;project&quot; simililar to
Microcal&nbsp;Origin 4.</DT>

<DT>&nbsp;</DT>

<DT>Best Regards&nbsp;<BR>
Lars Thomsen<BR>
--&nbsp;<BR>
Lars Thomsen, MSc. PhD<BR>
115 South Oval, Hamilton, L8S1R2 Ontario Canada<BR>
email lthomsen@interlynx.net<BR>
homepage http://home.interlynx.net/~lthomsen/index.htm<BR>
tlp 1 905 777 0720&nbsp; fax 1 905 777 0738<BR>
&nbsp;</DT>

</BODY>
</HTML>
------------18B62044158A7--

------------4D3F2E2B38B46
Content-Transfer-Encoding: 7bit
Content-Description: Address Book Card for Lars Thomsen
Content-Disposition: inline; filename="nsmail7F.TMP"
Content-Type: text/x-vCard; charset=us-ascii; name="nsmail7F.TMP"

BEGIN:VCARD
FN:Lars Thomsen
N:Thomsen;Lars
ORG:McMaster University
ADR:;;115 South Oval;Hamilton;Ontario;L8S1R2
EMAIL;INTERNET:lthomsen@interlynx.net
TITLE:MSc PhD
TEL;WORK:1 905 5259140 x22584
TEL;FAX:1 905 7770720
TEL;HOME:1 905 7770738
X-NAV-HTML:T
END:VCARD


------------4D3F2E2B38B46--


From owner-software@net.bio.net Sat Feb 08 22:00:00 1997
Path: biosci!rutgers.rutgers.edu!uwm.edu!newsfeeds.sol.net!nntp.uio.no!news.apfel.de!news-fra1.dfn.de!news-koe1.dfn.de!main.Germany.EU.net!Dortmund.Germany.EU.net!gsf.de!news
From: Burkhard Morgenstern <morgenst@gsf.de>
Newsgroups: bionet.software
Subject: New alignment program DIALIGN
Date: Fri, 07 Feb 1997 12:35:16 +0100
Organization: GSF Forschungszentrum fuer Umwelt und Gesundheit mbH
Lines: 48
Message-ID: <32FB1374.41C6@gsf.de>
NNTP-Posting-Host: ariane.gsf.de
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.0Gold (X11; I; OSF1 V3.2 alpha)

We would like to announce the availability of
an alignment program called

                  DIALIGN
                  *******

DIALIGN is a completely novel program for pairwise
as well as multiple alignment of nucleic acid and
protein sequences. It is especially suited to detect
local similarities in otherwise completely unrelated
sequences and it does not employ any kind of gap penalty.



The method is described in

  B. Morgenstern, A. Dress, T. Werner:
  Multiple DNA and protein sequence alignment based on
  segment-to-segment comparison
  Proc. Natl. Acad. Sci. 93 pp. 12098 - 12103 (1996)

DIALIGN executables for various UNIX platforms (DEC,
SGI, CONVEX, HP, SUN) together with a detailed user
guide, online help and a test example are available at

  http://www.gsf.de/biodv/dialign.html

or via ftp:

  ftp://ariane.gsf.de/pub/unix/dialign

More detailed information on DIALIGN is available at
our web page. If you have any questions, please feel
free to contact us by e-mail.

  Burkhard Morgenstern


----------------------------------------------------------

Dr. Burkhard Morgenstern
GSF - National Research Center for Environment and Health,
Institute of Biomathematics and Biometry
Ingolstaedter Landstr. 1,
85764 Neuherberg,
Germany

morgenstern@gsf.de

From owner-software@net.bio.net Sun Feb 09 22:00:00 1997
Path: biosci!daresbury!not-for-mail
From: Ivo Wiesner <nfix@jcu.cz>
Newsgroups: bionet.software
Subject: [Q]:your experience with DNAsis, PC-Gene, Omiga
Date: 10 Feb 1997 13:41:11 -0000
Lines: 11
Sender: lpddist@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <5dn8hn$25m@mserv1.dl.ac.uk>
MIME-Version: 1.0
Original-To: bio-software@dl.ac.uk

Dear Netter,

considering to buy some local sequence analysis software for IBM-PC
 I would much appreciate your recommendations and
experience, esp. about the  differences (for practical user) between
DNAsis, PC-Gene, OMIGA or other pckages?
What would be currently the point of choice? (considering  Pentium CPU)

Thank you for your replies directly to my address nfix@jcu.cz

Ivo Wiesner

From owner-software@net.bio.net Sun Feb 09 22:00:00 1997
Path: biosci!rutgers.rutgers.edu!uwm.edu!newsfeeds.sol.net!hunter.premier.net!uunet!in2.uu.net!198.168.54.138!rcogate.rco.qc.ca!altitude!usenet
From: Christine Dumas <cdumas@lynnon.qc.ca>
Newsgroups: bionet.software
Subject: Re: Windows: sequence analysis
Date: Mon, 10 Feb 1997 09:06:47 -0500
Organization: Lynnon BioSoft
Lines: 21
Message-ID: <32FF2B77.6AC2@lynnon.qc.ca>
References: <5cpibd$jnl$1@mark.ucdavis.edu> <32F7948C.79E@molbiol.uct.ac.za>
Reply-To: cdumas@lynnon.qc.ca
NNTP-Posting-Host: dynppp-28.hip.cam.org
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.0 (Win95; I)
CC: eimert@geisenheim.fa.fh-wiesbaden.de

Ed Rybicki wrote:
> Hi - we have recently started to use DNAMAN from Lynnon Biosoft up in
> Canada; it is remarkably full-featured (if a bit idiosyncratic), and
> will do multiple sequence alignment, translation, dna frag assembly, PCR
> primer and oligo design...it is also CHEAP ($260 or so).  Works well
> under Win 3.1 and is LIGHTNING fast on even long sequence multiple
> alignments.  Also does shading of alignments (another perennial q).
> --

Here is the address of Lynnon BioSoft:

Lynnon BioSoft
88, rue Adam
Vaudreuil, Quebec
Canada J7V 8P5

Tel: (514) 455-4894
Fax: (514) 455-4269

email:     mail@lynnon.qc.ca
support:   support@lynnon.qc.ca

From owner-software@net.bio.net Sun Feb 09 22:00:00 1997
Path: biosci!rutgers.rutgers.edu!uwm.edu!news-peer.gsl.net!news.gsl.net!howland.erols.net!rill.news.pipex.net!pipex!uknet!usenet1.news.uk.psi.net!uknet!uknet!newsfeed.ed.ac.uk!singer.cent.gla.ac.uk!lenzie!gpaa29
From: gpaa29@lenzie (Francis L.Burton)
Newsgroups: bionet.software
Subject: Re: What is your favorite patch-clamp hardware/software?
Date: 10 Feb 1997 10:06:01 GMT
Lines: 16
Message-ID: <5dmru9$2u9@singer.cent.gla.ac.uk>
References: <32f99325.3279390@news.uni-marburg.de> <32FC80D3.3EC1@interlynx.net>
NNTP-Posting-Host: lenzie.cent.gla.ac.uk
X-Newsreader: NN version 6.5.0 #18 (NOV)

I don't know what my favourite hardware/software would be
if I was still working in the patch-clamping field. I used 
EPC-7 with analysis software I wrote myself -- it got the
job done.

The most impressive system I ever saw was ISO2 which was
demoed by its creator M. Friedrich at the 1993 IUPS meeting
in Glasgow. It was (is?) a fast multitasked system allowing
simultaneous voltage pulse generation, digitization and
display. It had a pretty comprehensive set of analysis 
functions. The main flaw in the design was its dependence
on particular a A/D and high resolution display card, though 
that allowed it to leave the competition in the dust in
terms of performance.

Francis 

From owner-software@net.bio.net Sun Feb 09 22:00:00 1997
Path: biosci!rutgers.rutgers.edu!uwm.edu!news-peer.gsl.net!news.gsl.net!howland.erols.net!rill.news.pipex.net!pipex!oleane!in2p3.fr!swidir.switch.ch!serra.unipi.it!mikasa.iol.it!news
From: edoardo.shockwav@iol.it (Edo)
Newsgroups: bionet.software
Subject: Re: male and female symbol
Date: Mon, 10 Feb 1997 09:53:22 GMT
Organization: Italia Online
Lines: 12
Message-ID: <32fe0861.4006105@news.iol.it>
References: <32fc8e3a.2129086@news.iol.it>
NNTP-Posting-Host: 194.20.26.70
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Newsreader: Forte Agent .99g/32.339

I finally found it!

Thank you to all the kind people that wrote me.

Edo

On Fri, 07 Feb 1997 20:56:18 GMT, edoardo.shockwav@iol.it (Edo) wrote:

>I am looking for a TTF (True Type Font, for 