From owner-bio-software@hgmp.mrc.ac.uk  Mon Nov  1 03:11:35 1999
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From: amplicom@my-deja.com
X-Newsgroups: bionet.software
Subject: GeneLinkDB online database
Date: Mon, 01 Nov 1999 02:56:05 GMT
Organization: Deja.com - Before you buy.
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GeneLinkDB, a knowledge database system, is now up and running at a
bioinformatics portal http://www.amplicom.com

GeneLinkDB provides a comprehensive and searchable informations of
genes, proteins, associate diseases, publications and reagents.

We are constantly adding new informations and improving our web
contents. If you have any suggestions, please feel free to contact me at
webmaster@amplicom.com


Sent via Deja.com http://www.deja.com/
Before you buy.


From owner-bio-software@hgmp.mrc.ac.uk  Mon Nov  1 07:35:20 1999
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From: J G <gj254b@yahoo.com>
X-Newsgroups: alt.angst,alt.aol-sucks,alt.auqria,bionet.neuroscience,bionet.plants,bionet.software
Subject: Get rich while doing nothing
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Are you getting paid while surfing yet?
Are you only getting paid from one company?

If your answer to these is NO, please check the web site
http://www.geocities.com/gj254b/getpaid.html






From owner-bio-software@hgmp.mrc.ac.uk  Mon Nov  1 13:10:30 1999
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From: nospam@nospam.net
X-Newsgroups: bionet.software
Subject: Sanger Centre Image?
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Is there anyone out there who has gotten the Image program from the Sanger
Centre, the one for analysis of Bac digest fingerprints, to work on a Linux,
or a Digital, system?  I've tried 3.9a and 3.6 for Linux so far, and I'm
getting nothing but frustration.  Documentation is for 3.5.  I can't tell
what the settings mean because the new fields in 3.9 are undocumented.  The
program just gives error messages which seem to refer to these mystery
settings when tried.  I've just tried 3.6, and I get a "permission denied"
error when I try running bstt, or im3.  Root, user, no difference.  The
environment is set up as instructed.  The files are found on the path just
fine.  The standards file is a complete mystery.  The values and columns are
undefined.  Messages to image@sanger.ac.uk have not been answered for a week.

Is there other software for this task?  We'd like to arrange Bac clones by
their restriction digest fingerprints.

Thanks,
Mike Holloway
holloway.53@osu.edu


From owner-bio-software@hgmp.mrc.ac.uk  Mon Nov  1 15:30:27 1999
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From: Dyre Tjeldvoll <dyret@idi.ntnu.no>
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Subject: Re: Sanger Centre Image?
Date: 01 Nov 1999 16:25:46 +0100
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nospam@nospam.net writes:

> Is there anyone out there who has gotten the Image program from the Sanger
> Centre, the one for analysis of Bac digest fingerprints, to work on a Linux,
> or a Digital, system?  I've tried 3.9a and 3.6 for Linux so far, and I'm
> getting nothing but frustration.  Documentation is for 3.5.  I can't tell
> what the settings mean because the new fields in 3.9 are undocumented.  The
> program just gives error messages which seem to refer to these mystery
> settings when tried.  I've just tried 3.6, and I get a "permission denied"
> error when I try running bstt, or im3.  Root, user, no difference.  The
> environment is set up as instructed.  The files are found on the path just
> fine.  The standards file is a complete mystery.  The values and columns are
> undefined.  Messages to image@sanger.ac.uk have not been answered for a week.

If you get "permission denied" as root, the problem clould be that you 
need set execute permission for the file.

-- 
dt


From owner-bio-software@hgmp.mrc.ac.uk  Mon Nov  1 18:45:22 1999
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From: "Steve G" <steven.guntrip@virgin.net>
X-Newsgroups: alt.comp.freeware.gdp,alt.comp.shareware,alt.comp.software.tools,bionet.software,de.comp.shareware.misc,comp.lang.pascal.delphi.misc
Subject: Free software, chat, wallpaper and more on a coolwebsite - www.freespeech.org/gunsoft
Date: Mon, 1 Nov 1999 18:34:28 -0000
Organization: Gunsoft
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Our website which offers FREE PROGRAMS 4 all! has a new discussion page
where you can discuss absolutely anything you like!! So pay us a visit
www.freespeech.org/gunsoft ! U WILL NOT BE DISAPPOINTED!

--
>From Steve
<><><><><>
visit www.freespeech.org/gunsoft
icq #48322092

This isn't Spam as it was only posted on newsgroups talk about stuff related
to us!






From owner-bio-software@hgmp.mrc.ac.uk  Mon Nov  1 19:22:00 1999
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Subject: Download ASBSOLUTELY free software!
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Our website which offers FREE PROGRAMS 4 all! has a new discussion page
where you can discuss absolutely anything you like!! So pay us a visit
www.freespeech.org/gunsoft ! U WILL NOT BE DISAPPOINTED!

--
>From Steve
<><><><><>
visit www.freespeech.org/gunsoft
icq #48322092

This isn't Spam as it was only posted on newsgroups talk about stuff related
to us!




From owner-bio-software@hgmp.mrc.ac.uk  Mon Nov  1 21:37:34 1999
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From: "Nadeem Sheikh" <nadeem@bart.rprc.washington.edu>
X-Newsgroups: bionet.software
Subject: Plotting Packages
Date: Mon, 1 Nov 1999 13:37:03 -0800
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Does anyone have any advice for basic scientific plottng packages ?

I am currently a happy user of Origin (versin 3.5).  However I have been
charged with purchasing a cross platform package (for windows and mac
users), though origin is extremely user friendly and gives good output,
there is no mac version available (yet).  Hence any advice/poinetrs to which
would be a good package to opt for.

Many thanks in advance.

regards,

nadeem


Nadeem Sheikh,
****************************************************************************
************
RPRC                                                      Department of
Pharmaceutics
University of Washington                         University of Washington
Box 357331                                             Box 357610
Seattle, WA 98195                                  Seattle, WA 98195

voice : +1 206 221 2356
    fax : +1 206 956 0573
e-mail : nadeem@bart.rprc.washington.edu
http://www.rprc.washington.edu/




From owner-bio-software@hgmp.mrc.ac.uk  Mon Nov  1 22:35:19 1999
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From: g.dragicevic@medicine.unimelb.edu.au (George Dragicevic)
X-Newsgroups: bionet.software
Subject: Chemresearch150
Date: Mon, 01 Nov 1999 23:28:38 GMT
Organization: The University of Melbourne	
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I am searching for software (and a computer interface) which controls
the pouring of gradient gels. Isco used to produce a package and
interface called Chemresearch150 which did this job, however it is no
longer in production. Any leads as to where I could source this
software, or suggestions as to alternatives appreciated. Please
respond via email.
Thanks


From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  2 01:15:48 1999
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From: "Herbert M Sauro" <HSauro@fssc.demon.co.uk>
X-Newsgroups: bionet.metabolic-reg,bionet.software
Subject: ANNOUNCE:  New Experimental Metabolic Systems Lanaguage
Date: 1 Nov 1999 17:12:07 -0800
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I have uploaded a new and experimental metabolic systems language at

http://members.tripod.co.uk/sauro/biotech.htm under the Jarnac menu. The
software runs under Windows 95/98/NT4 and is currently supplied with only
limited documentation but numerous sample scripts.

Because Jarnac is very new, still undergoing changes and not fully tested,
it is being released into the public domain free of charge, however the
author still retains full copyright and exclusive commerical rights.

Some features

Jarnac can be used as a general purpose language for performing arithmetic
operations, including matrix and vector arithmetic, numerical
differentiation and integration and from within the Jarnac Windows front-end
a function or data grapher. Jarnac is an easy to learn control language,
similar to the Basic language and supports many of the constructs one would
expect,  for loops, conditionals, while/do and repeat/until. It supports at
least 7 different data types, including integers, floats, booleans, strings,
vectors, matrices and lists. Jarnac also supports user defined functions and
external modules.

There is built-in computational support for:

Describing and manipulating metabolic and cellular systems.
Dynamic Simulation (using LSODA integrator)
Steady State Analysis (using a modified Newton solver)
Simple Stability Analysis (eigenvalues)
Matrix Arithmetic (All the main operators including things like transpose,
det etc)
Metabolic Control Analysis (All steady state control coefficients and
elasticities)
Metabolic Structural Analysis.

Herbert Sauro







From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  2 03:43:05 1999
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From: pepper99@NOSPAM.home.com (Mark Morissette)
X-Newsgroups: alt.angst,alt.aol-sucks,alt.auqria,bionet.neuroscience,bionet.plants,bionet.software
Subject: Re: Get rich while doing nothing
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	plantbio@hgmp.mrc.ac.uk
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>Are you getting paid while surfing yet?
>Are you only getting paid from one company?
>
>If your answer to these is NO, please check the web site
>http://www.geocities.com/gj254b/getpaid.html

Geocities account cancelled, 'net access from Ohio State University
soon to follow!

Spam be gone!  :-)


---
Mark,  Oshawa, Ontario, Canada
pepper99@NOSPAM.home.com - Remove NOSPAM to respond via Email!
Send all spam to "send.spam.here@home.com" - Promptly forwarded to www.spamcop.net!  :-)


From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  2 11:45:37 1999
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Is it possible to get a copy too ???
Thank´s a lot!

Bernhard Roth
University of Hohenheim
General Virology
Germany


Paula Lavery schrieb in Nachricht
<2anS3.6117$6y2.141474@carnaval.risq.qc.ca>...
>Hi Martin
>
>If you give me your email address I will send you a copy of a database I
use
>as an inventory for all my cell lines in LN2. I tried to email you directly
>but the message was refused.
>
>Paula Lavery
>Transplant Immunology Lab.
>Royal Victoria Hospital
>McGill University
>Montreal (QC) Canada
>
>"If we knew what we were doing it wouldn't be called research, would it?"
>-- Albert Einstein
>
>Martin Offterdinger wrote in message <7vc4qa$19r6$1@www.univie.ac.at>...
>>Hi!
>>I just wonder if anyone used MS Access to handle cell lines in a database.
>>If yes I would be interested in receiving information how to design the
>>database.
>>Thank you very much!
>>Martin
>>
>>
>
>




From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  2 13:07:21 1999
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That is all nice, but is there a windows program for viewing and 
getting data from files made by ABI Genescan (for microsatellite 
analysis)?
Or does anyone know more about the format the data are stored in?

Jan Diblik




On 28 Oct 99, at 2:29, John Barlow wrote:

> A number of people asked about software that allows viewing of ABI trace files 
> (chromatograms) on the Windows platform.  There are quite many choices - some 
> programs allow viewing and printing only, others provide sophisticated tools 
> for contig assembly and proofreading.  Some programs are free (and very good - 
> see BioEdit), other cost several thousands of dollars and are difficult to use, 
> or have a confusing interface resulting from porting from the Mac.
> 
---


From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  2 14:05:27 1999
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From: wrp@alpha0.bioch.virginia.edu (William R. Pearson)
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Subject: Software for identifying new members of gene families
Date: 02 Nov 1999 08:54:30 -0500
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We have developed a simple computational/graphical strategy for
screening nightly updates of Genbank (actually we do the screens
weekly on the 7 nightly updates) for new members of large protein
families.

     Retief, J. D., Lynch, K. R., and Pearson, W. R. (1999)
     Panning for genes - a visual strategy for identifying novel
     gene orthologs and paralogs. Genome Res. 9:373-382.

The software is available free for academic users from
http://www.uvasoftware.org.

The strategy searches DNA databases using 20 - 60 protein query
sequences, which represent the different known branches of the protein
family, with tfastx3.  The 20 - 60 tfastx3 search results are then
scanned and rearranged and summarized graphically in a way that
greatly simplifies identification of new family members.  For an
example, see:

	 http://fasta.bioch.virginia.edu/fasta/pan_demo/gt_demo.pdf
	 http://fasta.bioch.virginia.edu/fasta/pan_demo/gtm_demo.pdf
	 http://fasta.bioch.virginia.edu/fasta/pan_demo/gstn_old.pdf
	 http://fasta.bioch.virginia.edu/fasta/pan_demo/gstn_new.pdf

If you view these pages using the acrobat plug-in to Netscape or IE,
you can click on each panel to see the underlying alignments.

I mention this because several investigators in this field were
unaware of the approach and were scanning for new gene family members
in a more cumbersome fashion.  The program is relatively easy to set
up if you are getting your data from the NCBI (e.g. est_human or
genbank nightly updates) and you have the fasta3 package of programs
from the University of Virginia.  People have had more difficulties
when using other databases, and it will not work with the GCG version
of FASTA.

Bill Pearson



From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  2 15:10:19 1999
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From: james.o.mcinerney@may.ie (James McInerney)
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Subject: Molecular Systematics Website
Date: 2 Nov 1999 07:03:42 -0800
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(Apologies for cross-posting)


Dear all,

Martin Embley, Mark Wilkinson, Robert Hirt and myself have conspired to
create a website that can be used as a teaching resource for Molecular
Systematics.  This has come about principally as a result of our
teaching efforts in conjunction with the Instituto Oswaldo Cruz, Rio de
Janeiro, Brazil and The Natural History Museum, London.

This website contains lecture material, both as internet presentations
and as a downloadable (?) Microsoft Powerpoint file with 344 slides. 
There is also a rudimentary glossary of terms that are used in the area
of molecular systematics, a list of internet resources and some
information about the participants, organisers and teachers on these courses.

There is no restriction on the use of this material.  Please feel free
to download the slides and use the site as you wish.  We would
appreciate an email, though, just to say you have visited and taken
something away.  We can then keep you abreast of developments and additions.

The site is currently mounted on two servers, but will soon be available
as part of The Natural History Museum, London main website.  It is being
re-branded so that it is recognisable as a product of the work of the museum.

The sites are:

Brazil:
http://www.dbbm.fiocruz.br/james/index.html

Ireland:
http://www.may.ie/academic/biology/jmbioinformatics.shtml

and choose the link for the molecular systematics course.

A portion of his course was also taught as part of this summers'
EMBO-funded Bioinformatics Course (Bioinformatics: From genome sequences
to protein structures) at Uppsala, Sweden:

http://xray.bmc.uu.se/EMBO


The site will soon be available on The Natural History Museum server.
http://www.nhm.ac.uk/

Please pass this email on to anybody that might conceivably be
interested.  If there are any problems, errors or ommissions please do
not hesitate to inform us.

Kindest regards,

James
-- 
             Dr. James O. McInerney,
Dept. Biology,                       Dept. Zoology,
Natl. Univ. Ireland,                 The Natural History Museum,
Maynooth,                  and       Cromwell road,
Co. Kildare, Ireland                 London SW7 5BD, UK.
Phone +353 1 708 3860                +44 171 938 9163
Fax   +353 1 708 3845                +44 171 938 9158
email james.o.mcinerney@may.ie       j.mcinerney@nhm.ac.uk
http://www.may.ie/academic/biology/jmbioinformatics.shtml


From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  2 16:15:37 1999
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From: Bill_A_Nussbaumer@ms.bd.com
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Bill A Nussbaumer@BDX
11/02/99 10:59 AM

I'm not familiar with ABI genescan so I don't know if this is quite what you
need or not.  But it should only take you a couple of minutes to figure it out.

Check out Chromas at :

http://www.technelysium.com.au/chromas.html

Hope it helps you,

Bill





That is all nice, but is there a windows program for viewing and
getting data from files made by ABI Genescan (for microsatellite
analysis)?
Or does anyone know more about the format the data are stored in?

Jan Diblik











From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  2 17:45:39 1999
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Bill A Nussbaumer@BDX
11/02/99 12:17 PM

Looked at the whole thread and this had already been mentioned.  Whoops.  :-)

Bill





I'm not familiar with ABI genescan so I don't know if this is quite what you
need or not.  But it should only take you a couple of minutes to figure it out.

Check out Chromas at :

http://www.technelysium.com.au/chromas.html

Hope it helps you,

Bill
















From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  2 19:19:16 1999
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From: "Steve G" <steven.guntrip@virgin.net>
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Subject: Free BRILLIANT software - www.freespeech.org/gunsoft
Date: Tue, 2 Nov 1999 19:15:09 -0000
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Check it out now, FREE SOFTWARE!! (About 20 downloads) and TONNES more on
the site!

www.freespeech.org/gunsoft

--
>From Steve
<><><><><>
visit www.freespeech.org/gunsoft










From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  2 20:05:23 1999
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Hi

You may want to check out a product called Prism from GraphPad software
(http://www.graphpad.com/).  We use this and it is a good graphing package.
>From your point of view it is available on both Mac and Windows platforms
although there are version differences.  You can also mount the Windows
version on a Network (Mac?)

Hope this helps

John


Nadeem Sheikh <nadeem@bart.rprc.washington.edu> wrote in message
news:7vl0v2$puk$1@nntp4.u.washington.edu...
> Does anyone have any advice for basic scientific plottng packages ?
>
> I am currently a happy user of Origin (versin 3.5).  However I have been
> charged with purchasing a cross platform package (for windows and mac
> users), though origin is extremely user friendly and gives good output,
> there is no mac version available (yet).  Hence any advice/poinetrs to
which
> would be a good package to opt for.
>
> Many thanks in advance.
>
> regards,
>
> nadeem
>
>
> Nadeem Sheikh,
>
****************************************************************************
> ************
> RPRC                                                      Department of
> Pharmaceutics
> University of Washington                         University of Washington
> Box 357331                                             Box 357610
> Seattle, WA 98195                                  Seattle, WA 98195
>
> voice : +1 206 221 2356
>     fax : +1 206 956 0573
> e-mail : nadeem@bart.rprc.washington.edu
> http://www.rprc.washington.edu/
>
>




From owner-bio-software@hgmp.mrc.ac.uk  Wed Nov  3 04:10:14 1999
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From: a.walden@wave.co.nz (Adrian Walden)
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I am installing standalone BLAST on a Pentium running redhat linux 6.1.
This is somewhoat of a challange for me as my previous experience
installing software etc is on a Macintosh. My most recent success's on
linux have included some minor improvments to my .bash_profile and .bashrc
files and the installation of the Staden Package (a great victory). You
should conclude from this that I am a beginner !

My question concerns a few problems I have had with blast and the
requirement to setup a file called .ncbirc in my root directory or the
directory from which I call the BLAST application. The contents of the
.ncbirc file are supposed to be

[ ncbi]
Data=/path/blast/data

I would like to know what this file does and why it is there. Is it
supposed to export the variable "Data" to the environment. If so, why is
it that when I query th environment with 

echo $Data

that I get a blank line instead of a path to the directory "data" ?

Thanks for your help

Adrian walden

a.walden@(delete)wave.co.nc


From owner-bio-software@hgmp.mrc.ac.uk  Wed Nov  3 05:35:27 1999
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www.sfu.ca/~loryan/neural.html




From owner-bio-software@hgmp.mrc.ac.uk  Wed Nov  3 08:55:30 1999
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From: chevreux@mbp-lserv1.inet.dkfz-heidelberg.de (Bastien Chevreux)
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Subject: ANNOUNCE: MIRA 1.3.17 shotgun assembler
Date: 3 Nov 1999 08:47:03 GMT
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            Deutsches Krebsforschungszentrum Heidelberg

                           MIRA V1.3.17

a DNA shotgun fragment assembler with integrated trace file analysis


MIRA is a DNA shotgun sequence data assembly program which takes
reads/sequences gained by (gel or capillary) electrophoresis
experiments and defined in the Staden experiment (EXP), Sanger CAF or
even FASTA file format as input and assembles them into contigs. SCF
signal electrophoresis trace files are used, if present, to adjudicate
between contradicting stretches of bases in reads. The resulting
assembly is being written as standard CAF file which can easily be
imported into numerous finishing tools like GAP4.

We provide two versions of MIRA. One uses the automatic contig editor
EdIt that has been trained on standard ABI traces (mira_j), the other
version has been trained on LICOR traces (mira_l). Currently supported
platforms are SGI IRIX64, SUN Solaris and Linux (i386).

The canonical homepage of MIRA and EdIt, including download
possibilities, is:

            http://www.dkfz-heidelberg.de/mbp-ased/

A complete documentation can be found in the 'doc' directory of the
tarballs as HTML, texinfo and man page. Please mail suggestions,
critics, bug reports etc. to b.chevreux@dkfz-heidelberg.de
t.pfisterer@dkfz-heidelberg.de

Have fun.

-------------------------------------------------------------------------------
   Dipl.-Inform. Med. Bastien Chevreux  --  b.chevreux@dkfz-heidelberg.de 
 Deutsches Krebsforschungszentrum Heidelberg -- Abteilung Molekulare Biophysik 
    Im Neuenheimer Feld 280 -- 69120 Heidelberg -- Phone: +49 6221 42 2336
-------------------------------------------------------------------------------
    -It's mine.  =Nope, it's my one.   *CRASH*   =Alright. It WAS yours.


From owner-bio-software@hgmp.mrc.ac.uk  Wed Nov  3 10:55:33 1999
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From: "Nanci E Donacki" <nancieed@erols.com>
X-Newsgroups: bionet.cellbiol,bionet.molbio.methds-reagnts,bionet.software
Subject: Re: MS Access for cell lines
Date: Wed, 3 Nov 1999 05:46:06 -0500
Lines: 51
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References: <7vc4qa$19r6$1@www.univie.ac.at> <2anS3.6117$6y2.141474@carnaval.risq.qc.ca> <7vmh9o$87g$1@newspc4.rz.uni-hohenheim.de>
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	methods@hgmp.mrc.ac.uk
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There is also a cell line database that is available for download at
http://www.protocol-online.net
in the cell culture section.
-ned

Bernhard Roth <roth@uni-hohenheim.de> wrote in message
news:7vmh9o$87g$1@newspc4.rz.uni-hohenheim.de...
| Is it possible to get a copy too ???
| Thank´s a lot!
|
| Bernhard Roth
| University of Hohenheim
| General Virology
| Germany
|
|
| Paula Lavery schrieb in Nachricht
| <2anS3.6117$6y2.141474@carnaval.risq.qc.ca>...
| >Hi Martin
| >
| >If you give me your email address I will send you a copy of a database I
| use
| >as an inventory for all my cell lines in LN2. I tried to email you
directly
| >but the message was refused.
| >
| >Paula Lavery
| >Transplant Immunology Lab.
| >Royal Victoria Hospital
| >McGill University
| >Montreal (QC) Canada
| >
| >"If we knew what we were doing it wouldn't be called research, would it?"
| >-- Albert Einstein
| >
| >Martin Offterdinger wrote in message <7vc4qa$19r6$1@www.univie.ac.at>...
| >>Hi!
| >>I just wonder if anyone used MS Access to handle cell lines in a
database.
| >>If yes I would be interested in receiving information how to design the
| >>database.
| >>Thank you very much!
| >>Martin
| >>
| >>
| >
| >
|
|




From owner-bio-software@hgmp.mrc.ac.uk  Wed Nov  3 11:58:55 1999
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From: Dyre Tjeldvoll <dyret@idi.ntnu.no>
X-Newsgroups: bionet.software
Subject: Re: LINUX/standalone blast question
Date: 03 Nov 1999 12:56:17 +0100
Organization: Norwegian university of science and technology
Message-ID: <epgk8nzpz4u.fsf@vier.idi.ntnu.no>
References: <a.walden-0311991709570001@p36.ak1.wave.co.nz>
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a.walden@wave.co.nz (Adrian Walden) writes:
[snip]
> 
> My question concerns a few problems I have had with blast and the
> requirement to setup a file called .ncbirc in my root directory or the
> directory from which I call the BLAST application. The contents of the
> .ncbirc file are supposed to be
> 
> [ ncbi]
> Data=/path/blast/data
> 
> I would like to know what this file does and why it is there. 

Many programs have a .programrc file, and I think (!) the rc stands
for resource, and these files are typically for saving settings. 

> Is it supposed to export the variable "Data" to the environment. If
> so, why is it that when I query th environment with
> 
> echo $Data
> 
> that I get a blank line instead of a path to the directory "data" ?

The line in your file sets the local shell-variable Data, not an
environment variable, if the file is treated as a shell-script. 

The difference is that a local variable is only known to shell in
which you set it, whereas an environment variable is inherited to the
processes started by the shell. You can test this by doing the
following:

dyret@vier~$ DUMMY=value
dyret@vier~$ bash
dyret@vier~$ echo $DUMMY

dyret@vier~$ exit
dyret@vier~$ echo $DUMMY
value
dyret@vier~$ export DUMMY=newvalue
dyret@vier~$ echo $DUMMY
newvalue
dyret@vier~$ bash
dyret@vier~$ echo $DUMMY
newvalue

It could of course be that the program itself exports the variable
after having read the file.


The conventional way to make an environment variable is to: 

1) Export the variable using the export command, i.e. 
   export Data=/path/blast/data

or

2) Declare the variable to be an environment variable:
	declare -x Data=/path/blast/data

Either is typically done in your .bashrc, .bash_profile (or .profile)

The bash man page, or alternatively the emacs info page, has more
info.

My advice is that you export Data on the command line (export
Data=/path/blast/data) and then run your program. If it works you
put the export command to your startup file, if not... well, then you
have a different problem. HTH.

-- 
dt


From owner-bio-software@hgmp.mrc.ac.uk  Wed Nov  3 15:14:03 1999
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From: Andrew Rambaut <andrew.rambaut@zoo.ox.ac.uk>
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Subject: Program to Estimate Rates of evolution for sequences...
Date: Wed, 03 Nov 1999 15:13:29 +0000
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I have just released a new software package that some people
may find useful (mainly people analysing viral sequences) when
sequences have been isolated at different points in time:

TipDate is an application for estimating the rate molecular evolution
(and hence a time-scale) for a phylogeny consisting of dated tips.
These will most frequently be from viruses or other fast-evolving
pathogens that have been isolated over a range of dates. The program
can also return the likelihood for the simple molecular clock model
(i.e., assuming that all sequences are contemporary) or the non-clock
model. These are useful for likelihood ratio tests of the fit of the
model to the data.

It is available at:

http://evolve.zoo.ox.ac.uk/TipDate/TipDate.html


From owner-bio-software@hgmp.mrc.ac.uk  Wed Nov  3 15:35:45 1999
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From: friard@molinette.unito.it (Olivier)
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Subject: Re: Primers design program from sequence alignment?
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Hi,

I wrote a DOS program to help to design primers on multi-alignment
(CLUSTALW format).
This program is called PRIMERS and you can find it at
http://annhyb.free.fr

I hope that this program will help you!
Bye

Olivier

>If there is any program or web resource which could make conservative
>PCR primers from DNA sequence alignments? Please let me know.

>Denis M.Larkin
>Institute of Cytology and Genetics
>Novosibirsk
>Russia
>



From owner-bio-software@hgmp.mrc.ac.uk  Wed Nov  3 15:45:28 1999
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From: jfryan@NHGRI.NIH.GOV (Joe Ryan)
X-Newsgroups: bionet.software
Subject: Re: LINUX/standalone blast question
Date: 3 Nov 1999 07:38:56 -0800
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Distribution: world
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You may want to have a look at 

    http://www.ncbi.nlm.nih.gov/BLAST/newblast.html
    http://genome.nhgri.nih.gov/blastall/blast_install/
    ftp://ncbi.nlm.nih.gov/blast/db/README

--Joe

On Wed, Nov 03, 1999 at 05:09:56PM +1300, Adrian Walden wrote:
> I am installing standalone BLAST on a Pentium running redhat linux 6.1.
> This is somewhoat of a challange for me as my previous experience
> installing software etc is on a Macintosh. My most recent success's on
> linux have included some minor improvments to my .bash_profile and .bashrc
> files and the installation of the Staden Package (a great victory). You
> should conclude from this that I am a beginner !
> 
> My question concerns a few problems I have had with blast and the
> requirement to setup a file called .ncbirc in my root directory or the
> directory from which I call the BLAST application. The contents of the
> .ncbirc file are supposed to be
> 
> [ ncbi]
> Data=/path/blast/data
> 
> I would like to know what this file does and why it is there. Is it
> supposed to export the variable "Data" to the environment. If so, why is
> it that when I query th environment with 
> 
> echo $Data
> 
> that I get a blank line instead of a path to the directory "data" ?
> 
> Thanks for your help
> 
> Adrian walden
> 
> a.walden@(delete)wave.co.nc
> 


From owner-bio-software@hgmp.mrc.ac.uk  Wed Nov  3 19:15:28 1999
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From: see@sig.for.address (Harry)
X-Newsgroups: bionet.software
Subject: Re: LINUX/standalone blast question
Date: Wed, 03 Nov 1999 11:11:55 -0800
Organization: University of California, Los Angeles
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In article <a.walden-0311991709570001@p36.ak1.wave.co.nz>,
a.walden@wave.co.nz (Adrian Walden) wrote:


> [ ncbi]
> Data=/path/blast/data

.ncbirc is a "settings" file used internally by NCBI's programs, ala
windows.ini or Mac/Preferences files. As far as I can tell, the settings
in this file don't alter your shell environment. I'm guessing they went
this route because not all operating systems that NCBI supports have an
"environment" in the Unix sense (MacOS comes to mind), so for
cross-platform issues, the rc file.

Nevertheless, I believe you can use environment variables on Unix-like
systems to substitute for .ncbirc. For example, with standalone blast,

   export $BLASTMAT=/path/blast/data

would accomplish the same as the .ncbirc lines above, I believe.

-Harry

-- 
Harry <h3 at ucla.edu>


From owner-bio-software@hgmp.mrc.ac.uk  Thu Nov  4 10:25:21 1999
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From: "Sales" <sales@chinaeast.com.hk>
X-Newsgroups: alt.hk,alt.marketing,alt.marketing.online,alt.med.software,alt.pcdos,alt.publish,alt.publish.books,alt.sex.cd-rom,alt.www.marketing,alt.www.marketing.adverts,aus.computers.cdrom,autodesk.mcad.marketing,bionet.software,bnr.software-eng
Subject: CDs replication
Date: Thu, 4 Nov 1999 17:59:44 +0800
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We are the CDs manufacturer in Hong Kong and we can provide the replication
of Audio CD, CD-ROM, CD-R, Super-VCD, Video CD, Wallet CD (Business name
card), Wonder CD (Irregular shaped CD) discs and the multimedia CD-ROM
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and DVD box.

We commit to provide our best quality services for processing from you
provided your master media to having up to 5 colors silk screen or offset
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(852) 2898-1082 or fax (852) 2898-1262 or email sales@chinaeast.com.hk We
are looking forward to co-operate with you in the future.

Yours Sincerely

Sales Department





From owner-bio-software@hgmp.mrc.ac.uk  Thu Nov  4 11:15:17 1999
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From: spam@127.0.0.1 (Bernard Murray, PhD)
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Subject: Re: LINUX/standalone blast question
Date: Wed, 03 Nov 1999 16:05:18 -0700
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In article <epgk8nzpz4u.fsf@vier.idi.ntnu.no>, Dyre Tjeldvoll
<dyret@idi.ntnu.no> wrote:

> Many programs have a .programrc file, and I think (!) the rc stands
> for resource, and these files are typically for saving settings. 

Actually I believe that the "rc" stands/used to stand for
"run control".

I am showing my age here.  I remember when the "ESC" key used
to stand for "Extended System Command" but there's no way you'll
be able to convince newcomers that it isn't "ESCape".

   Bernard

-- 
Bernard P. Murray, PhD
bpmurray at cgl . ucsf . edu
Department of Cellular & Molecular Pharmacology, UCSF


From owner-bio-software@hgmp.mrc.ac.uk  Thu Nov  4 13:35:18 1999
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From: Mike Sternberg <m.sternberg@icrf.icnet.uk>
X-Newsgroups: bionet.software
Subject: Postdoc Bioinformatics Data Mining London UK
Date: Thu, 04 Nov 1999 13:22:00 +0000
Organization: Imperial Cancer Research Fund
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Bioinformatics + Data Mining + London
Postdoctoral Fellow

£21,730 - £23,447
Initially 2 years Contract
London WC2A

A postdoctoral research position is available at the Imperial Cancer
Research Funds Biomolecular Modelling Laboratory in central London to
work
on the application of machine learning to discover rules governing
protein
structure.  The object is to use inductive logic programming and other
data
mining algorithms to find rules to assist in the prediction of protein
structure from sequence The project is supported by the United Kingdom
BBSRC/EPSRC Bioinformatics Initiative and the contract will initially be

for two years with possibility of subsequent extension for a third year.

The applicant must be skilled in computational approaches in
bioinformatics
and familiarity of protein structure will be advantageous.

For enquiries please contact Dr Mike Sternberg at
m.sternberg@icrf.icnet.uk.  For more information about the project see
http://www.bmm.icnet.uk/ilp.

Salary UK pounds 21,730 - 23,477 p.a. according to age and experience.






From owner-bio-software@hgmp.mrc.ac.uk  Thu Nov  4 14:45:16 1999
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From: Dyre Tjeldvoll <dyret@idi.ntnu.no>
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spam@127.0.0.1 (Bernard Murray, PhD) writes:
[snip]
> > Many programs have a .programrc file, and I think (!) the rc stands
> > for resource, and these files are typically for saving settings. 
> 
> Actually I believe that the "rc" stands/used to stand for
> "run control".

Ah..., well admittedly, rc is a poor abbreviation for resource. But
now I know... 

> I am showing my age here.  

I think I got that resource-idea from the fact that
many program settings can be modified by changing x-resources. I guess 
this shows my lack of age, since I have X as my point of reference... :)

> I remember when the "ESC" key used
> to stand for "Extended System Command" but there's no way you'll
> be able to convince newcomers that it isn't "ESCape".

I must admit I thought it was "Escape" too, but as a dedicated (X)emacs
user I like "Extended System Command" much better! 

I think the widespread use of PC's and DOS/Windows programs has
contributed to the "Escape" view, since those systems frequently use
"Esc" to exit from menus and programs.

-- 
dt


From owner-bio-software@hgmp.mrc.ac.uk  Thu Nov  4 21:45:31 1999
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From: see@sig.for.address (Harry)
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Subject: fasta against BLAST2.0 format library
Date: Thu, 04 Nov 1999 13:43:11 -0800
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I've been trying to get fasta33t00 to recognize a library/db in BLAST2.0
format (created by NCBI's formatdb, part of the standalone BLAST package).

If I create a fastlib file and point FASTLIB to it, the fasta programs
find and recognize the library fine. However, for my uses, I'd like to run
the fasta programs in a manner that doesn't depend on such a pointer file.
Instead, I'd like to point it at the library file on the command line.

For example:

   fasta33 query.fasta /path/to/blastdb

where the actual library files are blastdb.nsq, .nin, and .nhr.

This generates a "cannot open" error when it gets to the library. This
works fine if the library specified on the command line is in fasta format
so I tried setting LIBTYPE to 12, as described in the docs, to indicate it
is in BLAST2.0 format, but I still get the error.

Is there something I'm missing or is this just not possible and I am
misunderstanding the purpose of LIBTYPE?

Thanks,
Harry

-- 
Harry <h3 at ucla.edu>


From owner-bio-software@hgmp.mrc.ac.uk  Thu Nov  4 22:45:35 1999
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From: chevreux@mbp-lserv1.inet.dkfz-heidelberg.de (Bastien Chevreux)
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Subject: Announcement: MIRA 1.3.17 shotgun assembler
Date: 4 Nov 1999 14:32:19 -0800
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                           MIRA V1.3.17
  A DNA shotgung fragment assembler with integrated automatic editor

MIRA is a DNA shotgun sequence data assembly program which takes
reads/sequences gained by (gel or capillary) electrophoresis
experiments and defined in the Staden experiment (EXP), Sanger CAF or
even FASTA file format as input and assembles them into contigs. SCF
signal electrophoresis trace files are used, if present, to adjudicate
between contradicting stretches of bases in reads. The resulting
assembly is being written as standard CAF file which can easily be
imported into numerous finishing tools like GAP4.

We provide two versions of MIRA. One uses the automatic contig editor
EdIt that has been trained on standard ABI traces (mira_j), the other
version has been trained on LICOR traces (mira_l).

The canonical homepage of MIRA and EdIt, including download
possibilities, is:

            http://www.dkfz-heidelberg.de/mbp-ased/

A complete documentation can be found in the 'doc' directory of the
tarballs as HTML, texinfo and man page. Please mail suggestions,
critics, bug reports etc. to b.chevreux@dkfz-heidelberg.de and
t.pfisterer@dkfz-heidelberg.de

Have fun.

Salut,
       Bastien

-------------------------------------------------------------------------------
   Dipl.-Inform. Med. Bastien Chevreux  --  b.chevreux@dkfz-heidelberg.de 
 Deutsches Krebsforschungszentrum Heidelberg -- Abteilung Molekulare Biophysik 
    Im Neuenheimer Feld 280 -- 69120 Heidelberg -- Phone: +49 6221 42 2336
-------------------------------------------------------------------------------
    -It's mine.  =Nope, it's my one.   *CRASH*   =Alright. It WAS yours.



From owner-bio-software@hgmp.mrc.ac.uk  Fri Nov  5 09:25:28 1999
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From: crepinau@sb-roscoff.fr (Crepineau florent)
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Subject: from phrap to ReAligner
Date: 5 Nov 1999 09:24:31 GMT
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   Dear Neter,

I am looking for a software which could take an .ace file from phrap as an
input and transform it to a multiple alignment suitable for ReAligner and
which could use the multiple alignment resulting from ReAligner to
transforme it to a .ace file from phrap.

Does anybody has an idea?

Thanks in advance,

Yours sincerely

Florent

-- 
Florent Crépineau
Station Biologique de Roscoff
BP 74
29682 Roscoff cedex


From owner-bio-software@hgmp.mrc.ac.uk  Fri Nov  5 16:05:26 1999
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From: wrp@alpha0.bioch.virginia.edu (William R. Pearson)
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Subject: Re: fasta against BLAST2.0 format library
Date: 05 Nov 1999 10:49:40 -0500
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I've been trying to get fasta33t00 to recognize a library/db in BLAST2.0
format (created by NCBI's formatdb, part of the standalone BLAST package).
 ... 
I'd like to point it at the library file on the command line.

For example:

   fasta33 query.fasta /path/to/blastdb

where the actual library files are blastdb.nsq, .nin, and .nhr.

================
The solution is to put the library type in the library file name argument
like this:

   fasta33 query.fasta "/path/to/blastdb 12"

Bill Pearson


From owner-bio-software@hgmp.mrc.ac.uk  Fri Nov  5 16:35:28 1999
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From: =?iso-8859-1?Q?T=F5nu?= margus <tmargus@ebc.ee>
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Subject: predicting termination signal in procaryotes?
Date: Fri, 05 Nov 1999 18:33:00 +0200
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Is there some software for predicting transcriptional termination
signals in procaryotes?

Many Thanks in Advance

Tonu Margus



From owner-bio-software@hgmp.mrc.ac.uk  Fri Nov  5 18:05:26 1999
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From: see@sig.for.address (Harry)
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Subject: Re: fasta against BLAST2.0 format library
Date: Fri, 05 Nov 1999 10:01:23 -0800
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In article <kjd7tp0wh7.fsf@alpha0.bioch.virginia.edu>,
wrp@alpha0.bioch.virginia.edu (William R. Pearson) wrote:

> The solution is to put the library type in the library file name argument
> like this:
> 
>    fasta33 query.fasta "/path/to/blastdb 12"

Thanks Bill - of course that did the trick. I tried that w/o the quotes
and am now kicking myself.

-Harry

-- 
Harry <h3 at ucla.edu>


From owner-bio-software@hgmp.mrc.ac.uk  Fri Nov  5 18:15:16 1999
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From: "Steve G" <steven.guntrip@virgin.net>
X-Newsgroups: alt.comp.freeware.gdp,alt.comp.shareware,alt.comp.software.tools,bionet.software,de.comp.shareware.misc,comp.lang.pascal.delphi.misc
Subject: Absolutely no-cost, free software!
Date: Fri, 5 Nov 1999 18:08:16 -0000
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www.freespeech.org/gunsoft
Visit the above site 4 brilliant software! which is free to you! and u can
download it! talk to other people! get wallpaper! chat! and check out cool
links!






From owner-bio-software@hgmp.mrc.ac.uk  Fri Nov  5 20:45:28 1999
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From: Roland Mainz <roland.mainz@informatik.med.uni-giessen.de>
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Subject: Re: Software for viewing (and editing) ABI files on Windows
Date: Fri, 05 Nov 1999 21:31:48 +0100
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Hi !

----

Jan Diblik wrote:

> That is all nice, but is there a windows program for viewing and
> getting data from files made by ABI Genescan (for microsatellite
> analysis)?
> Or does anyone know more about the format the data are stored in?

Yup, me. I'm working on an import library for ABI, SCF, XSD etc. written in JAVA.
Drop me a mail if there's any interest...

----

Bye,
Roland

--
  __ .  . __
 (o.\ \/ /.o) Roland.Mainz@informatik.med.uni-giessen.de
  \__\/\/__/  gisburn@informatik.med.uni-giessen.de
  /O /==\ O\  MPEG specialist, C&&JAVA&&Sun&&Unix programmer
 (;O/ \/ \O;) TEL +49 (0) 641/99-13193 FAX +49 (0) 641/99-41359





From owner-bio-software@hgmp.mrc.ac.uk  Sat Nov  6 10:14:30 1999
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We let YOU download tonnes of stuff FOR FREE! Including software, quake2
maps, fonts, wall-paper and More!!!!!!!!!!




From owner-bio-software@hgmp.mrc.ac.uk  Sat Nov  6 11:39:19 1999
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www.freespeech.org/gunsoft
The NUMBER 1 place for FREE SOFTWARE




From owner-bio-software@hgmp.mrc.ac.uk  Sat Nov  6 17:18:21 1999
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From: gyy@tc.umn.edu (Guo-Yun Yu)
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Subject: MS Access for small # of EST, any suggestion?
Date: 6 Nov 1999 17:15:55 GMT
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Hi,

Any body have build a Access database to handle small EST project (about 
1000)?  If possible, could you let us share with you.  We'd appreciate your 
help very much. We welcome any suggestion.

Guo-Yun Yu
Department of Agronomy and Plant Genetics
University of Minnesota
email: gyy@tc.umn.edu



From owner-bio-software@hgmp.mrc.ac.uk  Sun Nov  7 02:45:16 1999
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From: hcwang@WH.MATH.UWATERLOO.CA (Huai-chun Wang)
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Subject: multiple alignment of text files
Date: 6 Nov 1999 18:33:22 -0800
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Hello,

I wonder whether there are softwares that do multiple alignment of any
plain text files in addition to DNA or protein sequence file. I tested
ClustalW but it can only align legal DNA base or amino acid residues,
and igonore all other English characters and symbols that typically
occured in an English text. Does any one suggest such a program for this
new need? Thank you.

Huaichun Wang
University of Waterloo





From owner-bio-software@hgmp.mrc.ac.uk  Sun Nov  7 08:17:51 1999
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From: Greg <jquinn@shell7.ba.best.com>
Subject: XML interfaces; BioML/BSML
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I'm currently designing an XML interface to a protein database 
system, and I'm finding it difficult to find any real-life
examples, aside from software produced by either ProteoMetrics
and/or Vidual Genomics, of who/what is using BioML or BSML.
Any suggestions?
Cheers
Greg


From owner-bio-software@hgmp.mrc.ac.uk  Sun Nov  7 17:55:17 1999
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Greg wrote:
> 
> I'm currently designing an XML interface to a protein database
> system, and I'm finding it difficult to find any real-life
> examples, aside from software produced by either ProteoMetrics
> and/or Vidual Genomics, of who/what is using BioML or BSML.
> Any suggestions?
> Cheers
> Greg

http://ala.vsms.nottingham.ac.uk/biodom/software/
http://204.112.55.140/BIOML/index.html


From owner-bio-software@hgmp.mrc.ac.uk  Mon Nov  8 04:17:51 1999
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From: amartin@stagleys.demon.co.uk (Andrew Martin)
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Subject: Re: LINUX/standalone blast question
Date: Sun, 7 Nov 1999 21:38:58 GMT
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Dyre Tjeldvoll (dyret@idi.ntnu.no) wrote:
: a.walden@wave.co.nz (Adrian Walden) writes:
: > Is it supposed to export the variable "Data" to the environment. If
: > so, why is it that when I query th environment with
: > 
: > echo $Data
: > 
: > that I get a blank line instead of a path to the directory "data" ?

: The line in your file sets the local shell-variable Data, not an
: environment variable, if the file is treated as a shell-script. 

: The difference is that a local variable is only known to shell in
: which you set it, whereas an environment variable is inherited to the
: processes started by the shell. You can test this by doing the
: following:

NO! It doesn't even set a local (shell) variable. The file is read
by the BLAST program not the shell, so it is used to set a parameter
internal to the program.

Andrew




From owner-bio-software@hgmp.mrc.ac.uk  Mon Nov  8 09:45:01 1999
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From: "Soeren W. Rasmussen" <swr@crc.dk>
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Subject: Re: MS Access for small # of EST, any suggestion?
Date: Mon, 08 Nov 1999 08:45:38 +0100
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Hi Guo-Yun Yu,

DNATools is a small software package (not an access database) which is designed
for this purpose. The program includes facilities for automated local and
global blast searching and information retrieval as well as clustal
multi-sequence alignment, restriction mapping, translation, comparing and
merging, re-naming files plus a number of other functions useful for handling
small (6-10,000) EST projects. Sequence annotation (e.g. blast search result)
is accessable for free text searching.

You can download it from http://www.crc.dk/phys/dnatools.htm and try it out. It
can be used free of charge.

Regards

Soeren

Guo-Yun Yu wrote:

> Hi,
>
> Any body have build a Access database to handle small EST project (about
> 1000)?  If possible, could you let us share with you.  We'd appreciate your
> help very much. We welcome any suggestion.
>
> Guo-Yun Yu
> Department of Agronomy and Plant Genetics
> University of Minnesota
> email: gyy@tc.umn.edu

--
Dr. scient. Søren W. Rasmussen
Carlsberg Laboratory, Department of Physiology
10 Gl. Carlsbergvej, DK-2500, Copenhagen, Denmark
Phone  45 33275230/45 36162259, Fax 45 33274766
swr@crc.dk, http://www.crc.dk/phys/

DNATools sequencing software:
http://www.crc.dk/phys/dnatools.htm




From owner-bio-software@hgmp.mrc.ac.uk  Mon Nov  8 10:33:42 1999
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http://members.tripod.lycos.nl/svenb/index.html




From owner-bio-software@hgmp.mrc.ac.uk  Mon Nov  8 10:52:58 1999
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From: Dyre Tjeldvoll <dyret@idi.ntnu.no>
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amartin@stagleys.demon.co.uk (Andrew Martin) writes:

[snip]
> : The line in your file sets the local shell-variable Data, not an
> : environment variable, if the file is treated as a shell-script. 
                          ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^

I added this condition because I have never used the program in
question. 
 
> NO! It doesn't even set a local (shell) variable. The file is read
> by the BLAST program not the shell, so it is used to set a parameter
> internal to the program.

I will take your word for it. I just guessed (wrongly, it appears),
that the file in question was a shell script that was sourced by
the program since the syntax of the snippet resembled that of a(n)
(ba)sh script.

-- 
dt


From owner-bio-software@hgmp.mrc.ac.uk  Mon Nov  8 15:25:24 1999
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From owner-bio-software@hgmp.mrc.ac.uk  Mon Nov  8 20:14:03 1999
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From: jfryan@NHGRI.NIH.GOV (Joe Ryan)
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Subject: Re: Alu Sequences
Date: 8 Nov 1999 11:56:28 -0800
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Check RepBASE 
    www.girinst.org

Joe


On Mon, Nov 08, 1999 at 10:22:07AM -0800, Yong Li wrote:
> Hi,
> 
> I need mouse Alu family sequences. Does anybody know where can I
> easily get them?
> 
> Thanks in advance.
> 
> Yong Li
> 
> 
> 


From owner-bio-software@hgmp.mrc.ac.uk  Mon Nov  8 20:47:24 1999
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From: acad@ACCEFYN.ORG.CO (AGC)
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para hispanoparlantes, agradecere sus comentarios, aun no esta
completamente montados los files, pero algo esta, todos sus comentarios
son bienvenidos, gracias.

http://www.accefyn.org.co/bioinfo/indice.html



From owner-bio-software@hgmp.mrc.ac.uk  Mon Nov  8 22:09:54 1999
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From: yong.li@UCHSC.EDU (Yong Li)
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Subject: Alu Sequences
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Hi,

I need mouse Alu family sequences. Does anybody know where can I
easily get them?

Thanks in advance.

Yong Li




From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  9 05:02:42 1999
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From: Paul Adams <PDAdams@nospam.lbl.gov>
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Subject: Job Posting: Computer Systems Engineer I/II
Date: Mon, 08 Nov 1999 20:56:54 -0800
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Computer Systems Engineer I/II
Job PB/011299/CJO
CSE I $3605-$5717/Mo.
CSE II $4326-$7210/Mo.

Computational Crystallography Initiative,
Physical Biosciences Division,
Lawrence Berkeley National Laboratory, 
Berkeley, California.

NOTE: One vacancy exists. Classification will depend upon the
applicant's level of skills, knowledge, and abilities. This is a
one-year term appointment with the possibility of renewal contingent
upon continued funding. 

DUTIES: Essential -- Under the general direction of the principal
investigator of the Computational Crystallography Initiative, assist
with the development and implementation of a high-level crystallographic
computing language and numerical algorithms for a scientific
application. The overall objectives of the Computational Crystallography
Initiative include the design of a high-level crystallographic computing
language, implemented as an "interpreter" using common computer language
creation tools. The resulting computational architecture will create a
highly flexible and open environment in which to execute new algorithms
for the automation of the multi-wavelength anomalous diffraction method,
model building, structure refinement, and eventually all major aspects
of X-ray crystallography.
Essential for the CSE II -- In addition to the CSE I duties, the CSE II
will be expected to provide technical guidance to other group members
and to engage in independent research.

QUALIFICATIONS: Essential -- Candidates will be considered from across
all levels of computer systems engineering experience. Most important is
extensive experience with C/C++ and Javascript, PERL or a similar
scripting language. Familiarity with one or more of the following is
desirable: current language translation tools (e.g., PCCTS/ANTLR),
source code revision systems, API design, web-based interfaces. Marginal
-- Experience in development and implementation of numerical algorithms
for scientific applications. Familiarity with UNIX or Windows system
management.
Essential for the CSE II -- In addition to the qualifications for the
CSE I, the CSE II will have a proven ability to assume lead roles in
activities and to grasp complex problems and independently develop
solutions and will possess an in-depth background in software
development.

POSTING DATE: October 21, 1999.
CLOSING DATE: Open until filled.

Further details about the position can be obtained from Paul Adams
(principal investigator) by email to PDAdams@lbl.gov

Please submit one copy of your resume via email (our preferred method)
to: employment@lbl.gov (no attachments, please). Reference job number
PB/011299 in your cover letter. Or mail to: Lawrence Berkeley National
Laboratory, One Cyclotron Road, MS 937-0600, Berkeley, CA 94720. Or FAX:
510-486-5870. Visit the Berkeley Lab Web site at:
http://www.lbl.gov/CJO. Berkeley Lab is an AA/EEO employer.


From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  9 06:15:06 1999
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From: spam@127.0.0.1 (Bernard Murray, PhD)
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Subject: Re: multiple alignment of text files
Date: Mon, 08 Nov 1999 18:07:04 -0700
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In article <3824F286.16121ACC@wh.math.uwaterloo.ca>,
hcwang@WH.MATH.UWATERLOO.CA (Huai-chun Wang) wrote:

> Hello,
> I wonder whether there are softwares that do multiple alignment of any
> plain text files in addition to DNA or protein sequence file. I tested
> ClustalW but it can only align legal DNA base or amino acid residues,
> and igonore all other English characters and symbols that typically
> occured in an English text. Does any one suggest such a program for this
> new need? Thank you.

Clustal can take external alignment matrices.  You could create
one of your own (perhaps a simple "identity" style matrix) which
includes any characters you would like to consider valid.
I can't remember how Clustal handles invalid characters during
input (I believe they are simply ignored) so you would probably have
to change the source for that section.

The standard UNIX text utility "diff" might prove useful in this
situation but this is line-based so you would have to place each
"sequence" in a separate file with one character per line.
(I have never tried this approach).

If the degree of "homology" is low you could try programs which
look for statistically significant groups of characters.
Such programs can operate without pre-determined matrices.
I have used MEME from SDSC to identify motifs in groups of
sequences.

My personal preference would be to strip the sequences of invalid
characters (sed script) and then run Clustal with an identity
matrix.  This would give an indication of similarity but would
not tell you how good the punctuation is in the individual files.

   Bernard

-- 
Bernard P. Murray, PhD
bpmurray at cgl . ucsf . edu
Department of Cellular & Molecular Pharmacology, UCSF


From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  9 07:16:56 1999
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Subject: Job Posting: Computational Crystallography Scientist
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Computational Crystallography Scientist 
Job PB/011300/CJO
$3515-$7029/Mo.

Computational Crystallography Initiative,
Physical Biosciences Division,
Lawrence Berkeley National Laboratory, 
Berkeley, California.

NOTE: This is a one-year term appointment with the possibility of
renewal contingent upon continued funding.

DUTIES: Essential -- Under the general direction of the principal
investigator of the Computational Crystallography Initiative, assist
with the development and implementation of algorithms for automatic
protein structure determination based on multi-wavelength X-ray
diffraction data. The overall objectives of the Computational
Crystallography Initiative include the design of a high-level
crystallographic computing language, implemented as an "interpreter"
using common computer language creation tools. The resulting
computational architecture will create a highly flexible and open
environment in which to execute new algorithms for the automation of the
multi-wavelength anomalous diffraction (MAD) method, model building,
structure refinement, and eventually all major aspects of X-ray
crystallography. The successful candidate will assist with research on
all aspects of computational methods for protein structure
determination: heavy atom location, MAD phasing, density modification,
automatic electron density interpretation and structure refinement. Will
collaborate in preparation of manuscripts for scientific publication and
other reports and documentation as needed. It is expected that research
results are presented at group meetings, seminars and scientific
conferences.

QUALIFICATIONS: Essential -- Ph.D. or equivalent experience in
computational crystallography, biology, chemistry, physics, computer
science, mathematics or engineering. Experience in development and
implementation of scientific algorithms. Experience with at least two
programming languages from the following list: C/C++, Java, Javascript,
PERL, Python. Strong publication record in the area of computational
methods development or a related field. Marginal -- Familiarity with or
experience developing research proposals in the area of computational
crystallography.

POSTING DATE: October 21, 1999.
CLOSING DATE: Open until filled.

Further details about the position can be obtained from Paul Adams
(princical investigator) by email to PDAdams@lbl.gov

Please submit one copy of your resume via email (our preferred method)
to: employment@lbl.gov (no attachments, please). Reference job number
PB/011300 in your cover letter. Or mail to: Lawrence Berkeley National
Laboratory, One Cyclotron Road, MS 937-0600, Berkeley, CA 94720. Or FAX:
510-486-5870. Visit the Berkeley Lab Web site at:
http://www.lbl.gov/CJO. Berkeley Lab is an AA/EEO employer.


From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  9 13:17:25 1999
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From: "Emmett Power" <Emmett@MIB-Group.com>
X-Newsgroups: bionet.software
Subject: CEO WANTED FOR AI-BASED PHARMACEUTICAL START-UP
Date: Tue, 9 Nov 1999 12:46:22 -0000
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CEO WANTED FOR AI-BASED PHARMACEUTICAL START-UP

Our client is a start-up in the field of the application of artificial
intelligence-based knowledge management solutions for pharmaceutical and
biotechnology research and development. The company has an alliance with one
of the leading academic centres of excellence for computer-driven artificial
research in the world.

The company also has highly-experienced sales and technology directors in
place and an impressive list of non-executive directors. The company is now
negotiating with venture funders for funding and with a major pharmaceutical
company to develop and trial the product. We are now looking for a CEO to
complete the management team and to lead the company through the venture
funding, launch and flotation stages.

It is essential that the successful candidate carries credibility within
client organisations in the pharmaceutical and biotechnology sectors. As a
minimum requirement, he or she will have occupied a senior managerial
position, ideally within the pharmaceutical sector. By the nature of both
the industry and the product, the role will call for an individual of the
very highest intellectual calibre.


The CEO will receive an equity stake in the business and a
performance-related option package. The post will be based in the South East
of England. This is a chance to share in the wealth creation process and to
steer an exciting, intellectually-challenging business to flotation.

Please reply in the first instance by email or post with a resume to Emmett
Power, CEO, The MIB Group, 126 Aldersgate Street, London EC1A 4JQ, United
Kingdom, telephone: 0171 687 0352, fax: 0171 687 0358, email:
Emmett@MIB-Group.com.

All applications will be treated in the strictest confidence and will be
used for no other purpose than consideration for the post described above.

**Please excuse any inappropriate or cross-postings**






From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  9 14:57:03 1999
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From: zw2@duke.edu (Zhong Wang)
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Subject: Need help for Software that can do codon usage analysis
Date: Tue, 09 Nov 1999 14:59:30 GMT
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Hi,

Anybody knows about software that can do codon usage analysis? I remember MacVector can do it, this way it is much easier to pick up a frameshift mutation in your 
DNA sequence. But I am working on IBM PC, there should be a software like that. 
Thanks.



From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  9 16:26:12 1999
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Subject: Re: Need help for Software that can do codon usage analysis
Date: Tue, 09 Nov 1999 10:40:41 -0500
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How about a web site?

Try:

http://www.kazusa.or.jp/codon/

Zhong Wang wrote:

> Hi,
>
> Anybody knows about software that can do codon usage analysis? I remember MacVector can do it, this way it is much easier to pick up a frameshift mutation in your
> DNA sequence. But I am working on IBM PC, there should be a software like that.
> Thanks.

--
Richard J. Dudley (rdudley+@pitt.edu)
Research Specialist V
Cystic Fibrosis Research Center
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
---> search BIONET archives at http://www.bio.net <---




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E' in vendita FIFA 2000 ad un prezzo bassissimo e conveniente per tutti
quelli che non vogliono spendere molto.
Per contattarci scrivete a sdavide@libero.it o backy@freemail.it
Sito internet http://backy.webjump.com







From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  9 18:15:15 1999
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From: "Steve G" <steven.guntrip@virgin.net>
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Free-software, discussion, MP3 music, chat, links, wallpaper, quake2 maps,
fonts, awards offered AND EVEN MORE!! AT

www.freespeech.org/gunsoft


--
>From Steve
<><><><><>
visit www.freespeech.org/gunsoft
icq #48322092






From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  9 19:21:55 1999
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From: "Marcel Dinger" <marcel@ihug.co.nz>
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Subject: Re: Need help for Software that can do codon usage analysis
Date: Wed, 10 Nov 1999 08:14:52 +1300
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Zhong Wang <zw2@duke.edu> wrote in message news:1103_942159570@oboe...
> Hi,
>
> Anybody knows about software that can do codon usage analysis? I remember
MacVector can do it, this way it is much easier to pick up a frameshift mutation
in your
> DNA sequence. But I am working on IBM PC, there should be a software like
that.
> Thanks.

Try Genamics SoftwareSeek, http://genamics.com/software

Just enter the keywords "codon usage" and links to several programs will come
up.

Hope this helps.

Marcel Dinger,
Genamics.






From owner-bio-software@hgmp.mrc.ac.uk  Tue Nov  9 21:25:22 1999
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From: "quaderno.com" <manfred@quaderno.com>
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Subject: QUADERNO The Musical Expression.
Date: Tue, 9 Nov 1999 22:21:41 +0100
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Greetings,

The following is a text-only description of a new program for music
composition. Please don't hesitate to download the demo version on the
Web at  http://www.quaderno.com


QUADERNO > THE  MUSICAL EXPRESSION

QUADERNO is a program for MIDI sequencing and an aid to musical
composition and research.
It runs on PC  (or on Power Mac using