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From: mpss3@AllThePlanet.com
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Subject: ADV: Stock Portfolio 2000 January Profile
Date: 1 Feb 2000 04:13:37 -0000
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Hello and welcome to the Stock Portfolio 2000 Newsletter.  If you no longer wish 
to receive this free newsletter, please visit http://63.86.208.127/remove.html

For more information, please visit http://63.86.208.127 or call toll free 
888-323-0856.

The greatest thing about the Internet is that it is the great equalizer in 
business and information resources. Look at the easiest example, a small website, 
which has now grown into an industry leader, started to sell books. They became so 
big so quickly that they made the industry leader in book sales change how and 
where they do business. The same is happening in all fields of business especially 
the music industry. More musicians than ever are getting broader exposure to new 
audiences that they could never reach before. The most innovative website on the 
music scene is MPEG Super Site, Inc.

MPEG Super Site, Inc. is a dynamic, comprehensive, music website that will be the 
measuring stick for all future websites. Utilizing the company's vast experience 
in the music industry, MPEG Super Site, Inc. will be an independent record label 
on the Internet, discovering, developing and promoting new artists in all the 
major genres of music. MPEG Super Site, Inc. is committed to signing the artists 
who have the ability to fulfill the niche market of the Internet generation. MPEG 
Super Site Inc. puts people who are hungry for new music in touch with free 
downloadable music, exclusive music news, concert information and album reviews on 
today's hottest musical acts. They also will have state of the art webcasts of 
special events on a global scale and a forum for direct interaction between artist 
and fan base. The MPEG Super Site will be user friendly for those who are new to 
the Internet. It will be sophisticated for the hardcore user. MPEG Super Site, 
Inc. is poised to redefine the way we purchase and listen to music into the next 
millennium.

MPEG Super Site, Inc. will compliment its Internet development with more 
traditional lines of business. A full service management company, MPEG represents 
both new and established musical artists, television and motion picture directors 
and choreographers, songwriters, producers and celebrities. As a rising force in 
film production, MPEG Super Site, Inc. is currently evaluating several production 
deals with high profile, established production companies for both television and 
theatrical release. MPEG Super Site, Inc. is a booking agent for several major 
entertainment venues in Las Vegas and Atlantic City. The company plans to support 
the sales of music CD's via the Internet with traditional record distribution. The 
company has an agreement in place with several international distributors.

MPEG Super Site, Inc. is clearly the cutting edge of the entertainment industry. 
At the present time no other entity brings together the product, the talent, the 
experience and the total package to the Internet industry like MPEG Super Site, 
Inc.

The Future of entertainment is through computers and the Internet. One company 
will emerge as the industry leader and take us into the next century of 
entertainment. The company that is positioned and poised to do so is MPEG Super 
Site, Inc.

Once again please visit http://63.86.208.127 or call toll free 888-323-0856.


Disclaimer
----------
This material is being provided by Stock Portfolio 2000, an electronic newsletter 
paid by the issuer for publishing the information contained in this report. Euro 
Media, Inc. has paid a consideration of 25,000 free trading shares of common stock 
of MPEG Super Site, Inc. to Stock Portfolio 2000 as payment for the publication of 
the information contained in this report. Stock Portfolio 2000 and its affiliates 
have agreed not to sell the common stock received as payment for its services 
until January 27, 2000, which date is 10 days from the initial dissemination of 
this report. After such date, Stock Portfolio 2000 may sell such shares in spite 
of any historical, current or future report or information conveyed about such 
securities. Because Stock Portfolio 2000 is paid for its services, there is an 
inherent conflict of interest in Stock Portfolio 2000's statements and opinions 
and such statements and opinions cannot be considered independent. The information 
contained in this publication is for informational purposes only, and not to be 
construed as an offer to sell or solicitation of an offer to buy any security. 
Please be advised that MPEG Super Site, Inc. is not offering securities for sale 
to persons in California or Minnesota. Stock Portfolio 2000 makes no 
representation or warranty relating to the validity of the facts presented nor 
does Stock Portfolio 2000 represent or warrant that all material facts necessary 
to make an investment decision are presented above. All statements of opinions are 
those of Stock Portfolio 2000. Stock Portfolio 2000 relies exclusively on 
information gathered from public filings on featured companies, as well as, in 
certain circumstances, interviews conducted by Stock Portfolio 2000 of management 
of featured companies. Investors should not rely solely on the information 
contained in this publication. Rather, investors should use the information 
contained in this publication as a starting point for conducting additional 
research on the featured companies in order to allow the investor to form his or 
her own opinion regarding the featured companies. Factual statements contained in 
this publication are made as of the date stated and they are subject to change 
without notice. Stock Portfolio 2000 is not a registered investment adviser, 
broker or a dealer. Investment in the companies reviewed is speculative and 
extremely high-risk and may result in the loss of some or all of any investment 
made in MPEG Super Site, Inc. Projections of future financial results are provided 
solely by MPEG Super Site, Inc. No assurances are given that MPEG Super Site, Inc. 
will achieve said projections. This publication contains forward-looking 
statements that are subject to risk and uncertainties that could cause results to 
differ materially from those set forth in the forward-looking statements. These 
forward-looking statements represent the judgment of MPEG Super Site, Inc. as of 
the date of this publication. The Company disclaims any intent or obligation to 
update these forward-looking statements.
 
 
 
 
 
 
 
 
 
---


From owner-bio-software@hgmp.mrc.ac.uk  Tue Feb  1 10:28:42 2000
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From: Tsunehisa Namba <drnamba@kuhp.kyoto-u.ac.jp>
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Subject: Software for Digital Video analisis
Date: Tue, 01 Feb 2000 19:25:23 +0900
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Hi all:

We are quantitating the motion of cilia by counting the recorded microscopic
images through CCD camera and digital video.  Since new iMac DV can handle
DV movie, I speculate it might be able to get the digital data of a specific
pixel on which a cilia moves.  In that way, we could automatically count the
frequency of cilia motion.

Are there any softwares (Mac) for this purpose?

Thanks

Nam
-- 
Tsunehisa Namba
Kyoto University Hospital
Sakyo-ku Kyoto 606-8507 JAPAN
E-mail: Drnamba@kuhp.kyoto-u.ac.jp



From owner-bio-software@hgmp.mrc.ac.uk  Tue Feb  1 15:00:55 2000
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From: Christian Richstein <poseidon@informatik.uni-bremen.de>
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Subject: Re: help on bioinformatics
Date: Tue, 01 Feb 2000 16:00:32 +0100
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Rein wrote:

> Dear Madam/Sir,
>
> We are students of the University of Leuven (Leuven) where we study Applied
> Economical Sciences. We're in the second grade now and some fellow students
> and we were instructed to write a book on bioinformatical software,
> algorithms & applications.
> The problem is that we have problems finding a structure in the
> bioinformatical world due to too much information. Another problem that has
> occured, is that we hardly have any knowledge of biology. We are stuck in a
> labyrinth and therefore I'm posting this to ask wether it would be possible
> if anyone can send us an overview of:
> 1. databases,
> 2. software,
> 3. other usefull information available.
>
> We would be very grateful for any kind of information.
> We thank you in advance and we are looking forward to hearing from you.
>
> Yours sincerely,
>
> Reinout Decock & Catherine Vertriest
> 2nd canditature Applied Economical Sciences,
> University of Leuven, Belgium
> reinout_decock@yahoo.com

Maybe the following homepages might help you:

http://www.ebi.ac.uk/
http://www.ncbi.nlm.nih.gov/
http://bibiserv.techfak.uni-bielefeld.de/
http://www.gensensorik.uni-bremen.de/glossar/glossar/

Good luck


poseidon



From owner-bio-software@hgmp.mrc.ac.uk  Tue Feb  1 15:12:48 2000
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From: Christian Richstein <poseidon@informatik.uni-bremen.de>
X-Newsgroups: bionet.software
Subject: BE CAREFUL WITH: "THIS IS THE" !!!!!! CHAINLETTER !!!!
Date: Tue, 01 Feb 2000 16:12:39 +0100
Organization: Universitaet Bremen, Germany
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I foud this message on the  bionet.softwae server and would advice everyone who reads this message, not to send any money to any of these names. This is the same whe all know with letters, anyway if you send a little bit of money or a choclate. These Chainletters are - in Germany- forbidden and for a good reason. These Internet Chainletters only overflow the memorys of the servers and the author of this text will be probably the only one who will profit from it. This is no story, it´s proved and if you don´t believe me, you might ask your parents, teachers or prof´s and I´m very sure, thy will tell you the same.


stigh@x-stream.no wrote:

>    THIS MAY BE THE MOST SIGNIFICANT LETTER YOU RECEIVE THIS YEAR!!
>
>    Hopefully, my name is still on the list. When I first read the letter, I thought it was some    off-the-wall idea to make money. A week later I met again with my client to discuss the issue.    I told him that the letter originally brought to me was not 100% legal. I advised him to make    a small change in the letter and it would be all right.
>
>
>    I was curious about the letter, so he told me how it works. I thought it was a long shot, so I    decided against participating. Before my client left, I asked him to keep me updated as to his    results. About two months later he called to tell me that he had received over $800,000 in      cash!! I didn't believe him so he asked me to try the plan and see for myself. I thought about    it for a few days and decided that there was not much to lose. I followed the instructions      exactly and mailed out 200 letters. Sure enough the money started coming in!! It came slowly    at first, but after three weeks I was getting more than I could open in a day. After about       three months the money stopped coming. I kept a precise record of my earnings and at the end    they totalled $868,439.00!
>
>
>    I was earning a good living as a lawyer, but as anyone in the legal profession will tell you,    there is a lot of stress that comes with the job. I told myself if things worked out I would     retire from practice and play golf. I decided to try the letter again, but this time I sent    out 500 letters. Well, three months after that I had totalled $2,344,178.00!! It seems       ridiculous and incredible but it is true.
>
>
>    I just couldn't believe it! I met my old client for lunch to find out exactly how this program    works. He told me that there were a few similar letters going around. What made this one         different is the fact that here are seven names on the letter, not five like most of the         others. This fact, alone, resulted more income. The other factor was the advice I gave him       in making sure the whole thing was perfectly legal, since no one wants to risk doing
>    anything illegal.
>
>    I bet now you are curious about what changes I told him to make. Well, if you send a letter      like this out, to be legal, you must sell something if you expect to receive a dollar. I told    him that anyone sending five dollars out must receive something in return. So when you send      three dollars to each of the seven names on the list you must include a piece of paper saying    "please put me on you mailing list" and include your name, email address and mailing address.
>
>
>    Follow these simple instructions exactly and in less than three months you will receive over     $800,000.00.
>
>    1) IMMEDIATELY send $3.00 to each of the seven names
>    listed below. The SOONER you send the "$3.00 LETTERS",
>    the SOONER you will start getting a return! Wrap the
>    dollar in a note saying: "Please add me to your mailing
>    list". Include YOUR NAME, MAILING ADDRESS, AND E-MAIL
>    ADDRESS. You will receive expert tips on promoting this
>    letter and some excellent BULK EMAIL resources.
>
>    *Remember...If you don't ask for this service, use of
>    this letter will be illegal for you!
>
>    1. Stig Hĺvard Henriksen, Moskomćlen, N-9151 Storslett, Norway
>
>    2. Aud Bekkmo, N-9151 Storslett, Norway
>
>    3. Eva Bekkmo, Malmvn. 136, N-9022 Krokelvdalen, Norway
>
>    4. Raymond Bekkmo, Rřyelen, N-9151 Storslett, Norway
>
>    5. Rolf Hauge, N-9151 Storslett, Norway
>
>    6. Raymond Hauge, N-9151 Storslett, Norway
>
>    7. Olfrid Beck, N-9151 Storlslett, Norway
>
>    2) REMOVE THE NAME & ADDRESS NEXT TO #1. at the top of
>    the list and MOVE the other names(#2 thru #7) UP ONE
>    POSITION. Then place YOUR NAME & ADDRESS in the #7 spot.
>    Be careful when you type the addresses. It is suggested
>    that you PROOFREAD to MAKE CERTAIN the names and
>    addresses are correct.
>
>    3) When you have completed the above instructions, you
>    may market the letter using the following options:
>
>    1. Bulk email
>    2. U.S. Postal Service
>    3. Flyers
>    4. Post FREE Classified Ads on the Internet
>    5. Newsgroups
>
>    This letter has been proven perfectly legal for all of
>    the above means, as long as you follow the instructions;
>    because you are purchasing membership in an exclusive
>    mailing list. To mail this letter out over the Internet,
>    you can browse through ours and find people to send it
>    to. All you have to do is cut and paste e-mail addresses
>    wherever you are on the Internet.
>
>    Another method of marketing the letter is using a Bulk
>    E-Mail Service to mail out letters in large volume for
>    you. We suggest using the Bulk E-Mail approach.
>    When you mail your $3 letters you will receive a
>    few recommendations for bulk email companies that
>    will supply you with fresh email addresses.
>
>    Posting FREE CLASSIFIED ADS on the Internet can also
>    achieve results. Simply go to a search engine(e.g. Yahoo,
>    Hot Bot, Lycos, Excite, Infoseek, etc. and type in FREE
>    CLASSIFIED ADS. You'll get a list of over 80,000 sites
>    where you can post ads. Remember to use a catchy title
>    like FREE MONEY, and post your e-mail address. When you
>    get an inquiry, simply e-mail a copy of this letter. The
>    more you send, the more you will make. It's a lot of
>    work...so that's why we recommend using a Bulk E-mail Service.
>
>    We strongly encourage you to mail this letter to your
>    family and friends. They'll be grateful!!
>
>    THIS IS A SERVICE AND IS 100% LEGAL. You may refer to:
>    Title 18, Section 1302 & 1342 of the U.S. Postal &
>    Lottery Statute; or check it out with the U.S. Postal
>    Service if you have questions.
>
>    Let's assume, for example, you get a 7.5% return rate. My
>    first attempt, however, was 9.5%, and my second was more than 11%.
>
>    1) When you send out 200 letters, 15 people send you $3.00 ($45.00)
>
>    2) Those 15 people mail out 200 letters and 225
>    people send you $3.00 ($675.00)
>
>    3) Those 225 people mail out 200 letters and 3,375
>    people send you $3.00 ($10,125.00)
>
>    4) Those 3,375 people mail out 200 letters, and
>    50,625 people send you $3.00 ($151,875.00)
>
>    5) Those 50,625 people mail out 200 letters, and
>    759,375 people send you $3.00 ($2,278,125.00)
>
>    6) At this level, your name drops off the list.
>
>    Think about it. Look at what you will have received
>    before your name drops off the list. It looks
>    unbelievable, I know. Do the math...see for yourself!
>    Just DO IT and you'll happily believe because you'll
>    receive proof in the form of MANY THREE-DOLLAR BILLS!!!
>
>    Just make certain that you send 3 dollar to each of the
>    seven names on the list; including the note asking to be
>    added to their mailing list. Together we will all prosper!
>
>
>    Remember a wise man saying:
>    It is not what you know that is important,
>    it is what you do with the things you know!
>
>    Well...you've read this far, so let me ask you two question:
>
>    Q: What do you have to lose?
>    A: Only $21.00
>
>    Q: What do you have to win?
>    A; If you do as instructed, at least $ 800,000
>
>
>
> ,
>
>
>
>
>
>
>
>
>
>
>
>
> ---



From owner-bio-software@hgmp.mrc.ac.uk  Tue Feb  1 20:55:15 2000
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Message-ID: <389747A8.27097E86@earthlink.net>
From: Al Viciedo <dood84@earthlink.net>
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Subject: WIN 98 SE for $15! AND MORE!
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ALL THIS SOFTWARE FOR *EACH* $10-15.

Delta Force 2- $13
Windows 98 SE-$15
Plus 98-$10
Sim City 3000-$13
Red Hat Linux 6.1-$10
Diablo-$9
Mcafee 2000 Toolbox-$9

These programs will come in there jewel cases, with all installation
software, and in perfect condition cd-rom. E-mail me at:
al_manson@hotmail.com



From owner-bio-software@hgmp.mrc.ac.uk  Tue Feb  1 23:16:10 2000
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From: madden@ncbi.nlm.nih.gov (Tom Madden)
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Subject: New NCBI toolkit and new BLAST binaries
Date: 1 Feb 2000 22:17:28 -0000
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A new version of the NCBI toolkit and new BLAST binaries have
been posted to the NCBI FTP site.  The toolkit is available at
ftp://ncbi.nlm.nih.gov/toolbox/ncbi_tools/ and the BLAST binaries 
are available at ftp://ncbi.nlm.nih.gov/blast/executables/


This release includes source code for vecscreen (in the 
demo directory). This program is a stand-alone version 
of the VecScreen system for identifying segments of a 
nucleic acid sequence that may be of vector origin (see 
www.ncbi.nlm.nih.gov/VecScreen/VecScreen.html). The 
vecscreen program uses the UniVec or UniVec_Core 
databases which are available at 
ftp://ncbi.nlm.nih.gov/pub/UniVec/.


Notes for BLAST 2.0.11 release:

Enhancements:

1.) Optimizations were contributed by Chris Joerg of COMPAQ.  These changes
reduce the number of cache misses, unroll loops, and make some instructions
unnecessary.  These improvements can speed up BLAST for long sequences
several-fold.

2.) A database is now only memory-mapped while being searched.  If multiple 
databases
are searched and the total exceeds the allowed memory-map limit this allows
all databases to be searched as memory-mapped files.  If a database cannot
be memory-mapped it is read as an ordinary file, rather than causing an error.

Bug fixes:

1.) Formatdb was fixed to correct a problem with FASTA string identifiers under 
NT.

2.) Blastpgp was fixed to prevent a core-dump under LINUX

3.) BLASTN was found to miss some hits near the expect value cutoff.  This has 
been
corrected.



---


From owner-bio-software@hgmp.mrc.ac.uk  Wed Feb  2 03:00:39 2000
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From: "Colin Smith" <colin@computersmith.com.au>
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Handy utility for portable computers at http://www.computersmith.com.au
(click on "Products" on the menu)

Features:

* Runs with icon in system tray.
* Displays user, computer and operating system information.
* Displays power source (mains or battery).
* Displays battery charge percentage (when running on battery)
* Displays battery status (OK, Low, Critical).
* Displays time elapsed since machine started.
* Displays time elapsed since machine switched to battery power.
* Displays percentage of battery charged when machine switched to
  battery power.
* Optionally logs all relevant events (system start, application start,
  application stop, battery start, battery stop).
* Produces on screen log reports which can also be printed.
* Log duration user configurable (in days or events).
* Low and Critical battery thresholds user configurable.

http://www.computersmith.com.au (click on "Products" on the menu)

--
Colin Smith
Computersmith Pty Ltd
Email: colin@computersmith.com.au
Web: http://www.computersmith.com.au






From owner-bio-software@hgmp.mrc.ac.uk  Wed Feb  2 05:26:13 2000
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Subject: protein expression and purification
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This is a multi-part message in MIME format.

------=_NextPart_000_00C9_01BF6D14.98A22580
Content-Type: text/plain;
	charset="iso-8859-2"
Content-Transfer-Encoding: quoted-printable

Do you have too many projects and limited time? If high level protein =
expression is one of your goals, C&P Biotech is ready to help you with =
sub-cloning and protein purification in E. coli.

    1.. Sub-cloning, site-directed mutagenesis, large deletion and =
insertion mutation.=20
    PRICE: US$ 600.00 per sample.

    2.. Protein expression and purification based on GST or =
histidine-tag fusion.
PRICE: US$ 1000.00, 5.0 mg electrophoresis pure protein is guaranteed.

Give yourself the chances to concentrate on other important projects =
with knowing that professionals are handling your expression and =
purification work.=20

NO CHARGE IF WE FAILED.

CONTACT ADDRESS:

Sue Han

C&P Biotech

3 Hampton Way

Thornhill, Ontario

Canada, L3T 5C8

Email: suehan@sprint.ca

Tel: 905 886 1898

Fax: 905 886 1898


------=_NextPart_000_00C9_01BF6D14.98A22580
Content-Type: text/html;
	charset="iso-8859-2"
Content-Transfer-Encoding: quoted-printable

<!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN">
<HTML>
<HEAD>

<META content=3D"text/html; charset=3Diso-8859-2" =
http-equiv=3DContent-Type>
<META content=3D'"MSHTML 4.72.3110.7"' name=3DGENERATOR>
</HEAD>
<BODY bgColor=3D#ffffff>
<DIV>
<DIV>
<P>Do you have too many projects and limited time? If high level protein =

expression is one of your goals, C&amp;P Biotech is ready to help you =
with=20
sub-cloning and protein purification in E. coli.</P>
<OL>
    <LI>Sub-cloning, site-directed mutagenesis, large deletion and =
insertion=20
    mutation.=20
    <P>PRICE: US$ 600.00 per sample.</P>
    <LI>Protein expression and purification based on GST or =
histidine-tag=20
    fusion.</LI></OL>
<P>PRICE: US$ 1000.00, 5.0 mg electrophoresis pure protein is =
guaranteed.</P>
<P>Give yourself the chances to concentrate on other important projects =
with=20
knowing that professionals are handling your expression and purification =
work.=20
</P>
<P>NO CHARGE IF WE FAILED.</P>
<P>CONTACT ADDRESS:</P>
<P>Sue Han</P>
<P>C&amp;P Biotech</P>
<P>3 Hampton Way</P>
<P>Thornhill, Ontario</P>
<P>Canada, L3T 5C8</P>
<P>Email: <A href=3D"mailto:suehan@sprint.ca"><FONT=20
size=3D2>suehan@sprint.ca</FONT></A></P>
<P>Tel: 905 886 1898</P>
<P>Fax: 905 886 1898</P></DIV></DIV></BODY></HTML>

------=_NextPart_000_00C9_01BF6D14.98A22580--

---


From owner-bio-software@hgmp.mrc.ac.uk  Wed Feb  2 09:28:08 2000
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From: "Dr. Duncan Clark" <Duncan@nospam.demon.co.uk>
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Subject: Re: protein expression and purification
Date: Wed, 2 Feb 2000 09:10:52 +0000
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In article <00cc01bf6d57$a7d1f380$af156395@hqian>, SUE HAN
<suehan@sprint.ca> writes
>    Do you have too many projects and limited time? If high level 
>    protein expression is one of your goals, C&P Biotech is ready to 
>    help you with sub-cloning and protein purification in E. coli.

The bionet newsgroup charter strictly forbids commercial advertising of
this nature. And why pick bionet.software anyway!

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk


From owner-bio-software@hgmp.mrc.ac.uk  Wed Feb  2 10:22:59 2000
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From: timour@itte.kz (Timour Ivashchenko)
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Subject: Help to find software for protein analysis
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Dear All!

Can anybody tell me where to get free software which can
calculate the following parameters
based on amino acid sequences:
1. Kyte&Doolittle Hydrophobicity priofile
2. Protein secondary structure

Sincerely,
Timour





---


From owner-bio-software@hgmp.mrc.ac.uk  Wed Feb  2 10:37:31 2000
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From: Nicolas Le Novere <nl223@cus.cam.ac.uk>
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Timour Ivashchenko wrote:

> Can anybody tell me where to get free software which can
> calculate the following parameters
> based on amino acid sequences:
> 1. Kyte&Doolittle Hydrophobicity priofile

Obsolete.

> 2. Protein secondary structure

Review on www.pasteur.fr/recherche/unites/neubiomol/secstrpr.html

With a lot of link. I suggest you to read some review of the 
subject before to use blindly the first program you find.

see for instance 
http://www.embl-heidelberg.de/predictprotein/doc/help_11.html#P11_more_papers
http://jura.ebi.ac.uk:8888/prot_html/
http://www.pasteur.fr/recherche/unites/neubiomol/ARTICLES/LENOV1999B/structsecond.html
(pdf available at
http://www.pasteur.fr/recherche/unites/neubiomol/ARTICLES/Lenov1999b.pdf)

Only one programm appeared performing better:

http://jura.ebi.ac.uk:8888/

Good luck,
 

--
Dr Nicolas Le Novčre                   e-mail: nl223@cus.cam.ac.uk 
Dpt Zoology, Univ Cambridge, Downing street, Cambridge CB2 3EJ, UK
http://www.pasteur.fr/recherche/unites/neubiomol/ 
tel: +44 1223 336623                          fax: +44 1223 336676


From owner-bio-software@hgmp.mrc.ac.uk  Wed Feb  2 12:04:08 2000
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Try new shareware utilities at
http://all4soft.netfirms.com


From owner-bio-software@hgmp.mrc.ac.uk  Wed Feb  2 17:09:21 2000
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I strongly disagree with Clark's comment.... advertising is allowed and it
is none of your fucking business Clark.
"Dr. Duncan Clark" wrote:

> In article <00cc01bf6d57$a7d1f380$af156395@hqian>, SUE HAN
> <suehan@sprint.ca> writes
> >    Do you have too many projects and limited time? If high level
> >    protein expression is one of your goals, C&P Biotech is ready to
> >.



From owner-bio-software@hgmp.mrc.ac.uk  Thu Feb  3 00:33:57 2000
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From: Kjeter@atmi.org (Kevin Jeter)
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I am not sure of the correct terminology.  Is there a source regarding this
topic?  I am also interested in any corporations that may be conducting this
sort of research.

Jeter
Manager, Economic Research
---


From owner-bio-software@hgmp.mrc.ac.uk  Thu Feb  3 07:10:20 2000
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From: spam@127.0.0.1 (Bernard Murray, PhD)
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In article <3897BBB4.C68DB1AF@biocomp.unl.edu>, Chris Larosa
<clarosa@biocomp.unl.edu> wrote:

> I strongly disagree with Clark's comment.... advertising is allowed and it
> is none of your [ELIDED} business Clark.

I suggest you do the following;

a) Consult RFC1855 Netiquette Guidelines.
   You will find that offensive messages are considered a breach of netiquette.
   eg. http://www.stanton.dtcc.edu/stanton/cs/rfc1855.html

b) Consult the charter for the bionet.* newsgroups.
   You will find that commercial activities (and advertising in particular)
are prohibited.
   eg. http://www.bio.net/docs/biosci.FAQ.html#q4

c) Calm down

   Bernard

-- 
Bernard P. Murray, PhD
bpmurray at cgl . ucsf . edu
Department of Cellular & Molecular Pharmacology, UCSF


From owner-bio-software@hgmp.mrc.ac.uk  Thu Feb  3 10:20:23 2000
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Dove trovo la tabella con le abbreviazioni per scaricare dalla rete le
quotazioni dei titoli che mi interessano?
Grazie





From owner-bio-software@hgmp.mrc.ac.uk  Thu Feb  3 11:28:19 2000
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From: timour@itte.kz (Timour Ivashchenko)
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Dear Dr Nicolas Le Novere!

Thank you for the links concerning protein secondary
structure prediction!
But if Kyte&Doolittle's Hydrophobicity priofile is obsolete
which one is up-to date now?
Any suggestions are wellcome!

Thanks in advance,
Timour




---


From owner-bio-software@hgmp.mrc.ac.uk  Thu Feb  3 13:39:15 2000
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From: t.j.keen@leeds.ac.uk (Jeffrey Keen)
Subject: Re: Help to find
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In article <002a01bf6e3a$55141040$4c8013d4@timour>, timour@itte.kz (Timour
Ivashchenko) wrote:

> Dear Dr Nicolas Le Novere!
> 
> Thank you for the links concerning protein secondary
> structure prediction!
> But if Kyte&Doolittle's Hydrophobicity priofile is obsolete
> which one is up-to date now?
> Any suggestions are wellcome!
> 

Try

http://www.hgmp.mrc.ac.uk/GenomeWeb/prot-transmembrane.html

I like

http://www.cbs.dtu.dk/services/TMHMM-1.0/


From owner-bio-software@hgmp.mrc.ac.uk  Thu Feb  3 13:43:14 2000
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From: "Dr. Duncan Clark" <Duncan@nospam.demon.co.uk>
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Subject: Re: Clarks comment: protein expression and purification
Date: Thu, 3 Feb 2000 09:30:40 +0000
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In article <3897BBB4.C68DB1AF@biocomp.unl.edu>, Chris Larosa
<clarosa@biocomp.unl.edu> writes
>I strongly disagree with Clark's comment.... advertising is allowed and
>it none of your ***** Clark

Another one for the kill file.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk


From owner-bio-software@hgmp.mrc.ac.uk  Thu Feb  3 16:58:32 2000
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In article <t.j.keen-0302001314370001@sjuh-pc151.leeds.ac.uk>,
Jeffrey Keen <t.j.keen@leeds.ac.uk> wrote:
>In article <002a01bf6e3a$55141040$4c8013d4@timour>, timour@itte.kz (Timour
>Ivashchenko) wrote:
>
>> Dear Dr Nicolas Le Novere!
>> 
>> Thank you for the links concerning protein secondary
>> structure prediction!
>> But if Kyte&Doolittle's Hydrophobicity priofile is obsolete
>> which one is up-to date now?
>> Any suggestions are wellcome!
>> 
>
>Try
>
>http://www.hgmp.mrc.ac.uk/GenomeWeb/prot-transmembrane.html

The topic is Secondary Structures!
Try:
http://www.hgmp.mrc.ac.uk/GenomeWeb/prot-2-struct.html
:-)

Which one is best?
Try one of the consensus methods, eg:
JPRED
http://jura.ebi.ac.uk:8888/

Gary Williams               Tel: +44 1223 494522  Fax: +44 1223 494512
mailto:G.Williams@hgmp.mrc.ac.uk            http://www.hgmp.mrc.ac.uk/
Bioinformatics,MRC HGMP Resource Centre,Hinxton,Cambridge, CB10 1SB,UK



From owner-bio-software@hgmp.mrc.ac.uk  Fri Feb  4 00:14:54 2000
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From: Chris Larosa <clarosa@biocomp.unl.edu>
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Aww, gee did I use a bad word... sorry....

There are so many advertisements (I love the ones coming from porno venders and
software pirates), that I just assumed that advertising was allowed.    Looks like
the administration of the bionet newsgroup is a joke to me.

I still think that legitimate and useful service advertisements germaine to biology
should be allowed, and I welcome seeing them on the bionet site.
PCPhD.

"Bernard Murray, PhD" wrote:

> In article <3897BBB4.C68DB1AF@biocomp.unl.edu>, Chris Larosa
> <clarosa@biocomp.unl.edu> wrote:
>
> > I strongly disagree with Clark's comment.....





From owner-bio-software@hgmp.mrc.ac.uk  Fri Feb  4 04:56:48 2000
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From: trevor@bwink.com (Trevor Bezdek)
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Subject: Java bioinformatics expert for contract?
Date: Fri, 04 Feb 2000 04:54:48 GMT
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Hi,

I'm looking for an individual with expertise in both Java and
bioinformatics to do a number of small contract projects.  These would
be things like implementing oligonucleotide dimer/hairpin energy
calculations, or computing similarity colorings for an alignment.
Local to Bay Area preferred.

Thanks, Trevor


From owner-bio-software@hgmp.mrc.ac.uk  Fri Feb  4 16:18:17 2000
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From: chevreux@mbp-lserv1.inet.dkfz-heidelberg.de (Bastien Chevreux)
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On Thu, 3 Feb 2000 09:30:40 +0000, Dr. Duncan Clark
<Duncan@nospam.demon.co.uk> wrote: 
>[...]
>Another one for the kill file.

You forgot to plonk him correctly. Please let me do this for you, will
you?

*PLONK*

Salut,
       Bastien

-------------------------------------------------------------------------------
   Dipl.-Inform. Med. Bastien Chevreux  --  b.chevreux@dkfz-heidelberg.de 
 Deutsches Krebsforschungszentrum Heidelberg -- Abteilung Molekulare Biophysik 
    Im Neuenheimer Feld 280 -- 69120 Heidelberg -- Phone: +49 6221 42 2336
-------------------------------------------------------------------------------
    -It's mine.  =Nope, it's my one.   *CRASH*   =Alright. It WAS yours.


From owner-bio-software@hgmp.mrc.ac.uk  Fri Feb  4 16:26:26 2000
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From: Chris Larosa <clarosa@biocomp.unl.edu>
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Subject: Re: Clarks comment: protein expression and purification
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Ouch...I deserved that.

Bastien Chevreux wrote:

>
>
> You forgot to plonk him correctly. Please let me do this for you, will
> you?
>
> *PLONK*
>
> Salut,
>        Bastien
>



From owner-bio-software@hgmp.mrc.ac.uk  Fri Feb  4 21:06:49 2000
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hello everybody,

i am a student in vienna and thinking right now of doing my final thesis =
on the bioacoustics of frogs (tropical). in my institute they are using =
CANARY for analysing the frog calls. but since i don=B4t have a mac i =
cannot use this on my labtop. is there anywhere a pc version of this =
programm?

thanks very much

sonja amoser


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<DIV><FONT face=3DArial size=3D2>hello everybody,</FONT></DIV>
<DIV>&nbsp;</DIV>
<DIV><FONT face=3DArial size=3D2>i am a student in vienna and thinking =
right now of=20
doing my final thesis on the bioacoustics of frogs (tropical). in my =
institute=20
they are using CANARY for analysing the frog calls. but since i don=B4t =
have a mac=20
i cannot use this on my labtop. is there anywhere a pc version of this=20
programm?</FONT></DIV>
<DIV>&nbsp;</DIV>
<DIV><FONT face=3DArial size=3D2>thanks very much</FONT></DIV>
<DIV>&nbsp;</DIV>
<DIV><FONT face=3DArial size=3D2>sonja amoser</FONT></DIV>
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From owner-bio-software@hgmp.mrc.ac.uk  Sun Feb  6 08:39:33 2000
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Does anyone know of good and free Oligonocleotide probe/PCR primer
design software for Windows/DOS?

I've had a look at Oligo5.0 and it looks excellent, but expensive

PS sorry for asking, I know this has been answered before, but I
deleted the post :==(

Thanks 
David
to reply via E-mail Replace no.span.ta with fsnet.co.uk
to reply via E-mail Replace no.span.ta with fsnet.co.uk


From owner-bio-software@hgmp.mrc.ac.uk  Sun Feb  6 15:25:47 2000
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hi..
   why don't you try "prime" on GCG. It is a great way to do it.  But you
can also try www.biowire.com
cheers
jakku

----- Original Message -----
From: David Barrass <david@barrass1.no.spam.ta>
To: <bio-software@hgmp.mrc.ac.uk>
Sent: Sunday, February 06, 2000 2:08 PM
Subject: Oligonucleotide Design


> Does anyone know of good and free Oligonocleotide probe/PCR primer
> design software for Windows/DOS?
>
> I've had a look at Oligo5.0 and it looks excellent, but expensive
>
> PS sorry for asking, I know this has been answered before, but I
> deleted the post :==(
>
> Thanks
> David
> to reply via E-mail Replace no.span.ta with fsnet.co.uk
> to reply via E-mail Replace no.span.ta with fsnet.co.uk
>

---


From owner-bio-software@hgmp.mrc.ac.uk  Mon Feb  7 02:40:20 2000
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From: Benny Shomer <bshomer@yahoo.com>
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Subject: Re: Oligonucleotide Design
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David,

The best experience I had is with MIT's primer3 program.
The resulting primers allways worked well in my hands and
the program provides many optional primer combinations.

http://www-genome.wi.mit.edu/cgi-bin/primer/primer3.cgi

Hope that helps,

Benny.


In article <389d3240.778032@news.freeserve.net>,
  david@barrass1.no.spam.ta (David Barrass) wrote:
> Does anyone know of good and free Oligonocleotide probe/PCR primer
> design software for Windows/DOS?
<..........>
--
----                 E pur si muove!   (Galileo)                 ----
---------------------------------------------------------------------
Benny Shomer, Ph.D.                     Bioinformatics FreeLancer
Tel#     +972 (8)  971-3165             email: bshomer@yahoo.com
Mobile#  +972 (54) 655-868              Fax#  +972 (15154) 655-868
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Sent via Deja.com http://www.deja.com/
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From owner-bio-software@hgmp.mrc.ac.uk  Mon Feb  7 02:50:11 2000
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Subject: Re: SEQIO package: question about idxseq
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Hi Catherine,


> Has someone already used the idxseq command included in the SEQIO
package?
> I cannot find where to contact the author of the package, who has
> apparently moved (James R. Knight,knight@cs.ucdavis.edu).

I used idxseq intensively and could probably answer specific
questions if needed.

Jim Knight is currently at CuraGen Co., jknight@curagen.com

Regards,

Benny.

--
----                 E pur si muove!   (Galileo)                 ----
---------------------------------------------------------------------
Benny Shomer, Ph.D.                     Bioinformatics FreeLancer
Tel#     +972 (8)  971-3165             email: bshomer@yahoo.com
Mobile#  +972 (54) 655-868              Fax#  +972 (15154) 655-868
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Sent via Deja.com http://www.deja.com/
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    i am a student in vienna and thinking right now of doing my final =
thesis on the bioacoustics of frogs (tropical). in my institute they are =
using CANARY for analysing the frog calls. but since i don=B4t have a =
mac i cannot use this on my labtop. is there anywhere a pc version of =
this programm?
    sonja amoser
   =20
        I'm not aware of a PC version of CANARY, but you might want to =
look at a spectrogram program downladable from
    http://www.monumental.com/rshorne/examples.html
   =20
    You will also need a sound card and means of connecting a recorder =
to it...
   =20
    JMPylka


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<!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN">
<HTML>
<HEAD>

<META content=3Dtext/html;charset=3Diso-8859-1 =
http-equiv=3DContent-Type><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 =
Transitional//EN">
<META content=3D'"MSHTML 4.72.3110.7"' name=3DGENERATOR>
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</HEAD>
<BODY bgColor=3D#ffffff>
<DIV>&nbsp;</DIV>
<BLOCKQUOTE=20
style=3D"BORDER-LEFT: #000000 solid 2px; MARGIN-LEFT: 5px; PADDING-LEFT: =
5px">
    <DIV><FONT face=3DArial size=3D2>i am a student in vienna and =
thinking right now=20
    of doing my final thesis on the bioacoustics of frogs (tropical). in =
my=20
    institute they are using CANARY for analysing the frog calls. but =
since i=20
    don&acute;t have a mac i cannot use this on my labtop. is there =
anywhere a=20
    pc version of this programm?</FONT></DIV>
    <DIV><FONT face=3DArial size=3D2>sonja amoser</FONT></DIV>
    <DIV><FONT face=3DArial size=3D2></FONT>&nbsp;</DIV>
    <DIV><FONT color=3D#000000 size=3D2>&nbsp;&nbsp;&nbsp; I'm not aware =
of a PC=20
    version of CANARY, but you might want to look at a spectrogram =
program=20
    downladable from</FONT></DIV>
    <DIV><FONT color=3D#000000 size=3D2></FONT><FONT size=3D2><A=20
    =
href=3D"http://www.monumental.com/rshorne/examples.html">http://www.monum=
ental.com/rshorne/examples.html</A></FONT></DIV>
    <DIV><FONT size=3D2></FONT>&nbsp;</DIV>
    <DIV><FONT color=3D#000000 size=3D2>You will also need a sound card =
and means of=20
    connecting a recorder to it...</FONT></DIV>
    <DIV><FONT color=3D#000000 size=3D2></FONT>&nbsp;</DIV>
    <DIV><FONT size=3D2>JMPylka</FONT></DIV>
    <DIV>&nbsp;</DIV></BLOCKQUOTE></BODY></HTML>

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david@barrass1.no.spam.ta (David Barrass) writes:

> Does anyone know of good and free Oligonocleotide probe/PCR primer
> design software for Windows/DOS?
> 
> I've had a look at Oligo5.0 and it looks excellent, but expensive
Hi,
try this site:
http://www.bioinformatik.de/cgi-bin/browse/Catalog/PCR/Primer_Design/

Andrea


-- 
Institut fuer Botanik III
Heinrich-Heine-Universitaet
Universitaetsstr.1         email : webmaster@bioinformatik.de 
40225 Duesseldorf          http://www.bioinformatik.de


From owner-bio-software@hgmp.mrc.ac.uk  Mon Feb  7 15:49:04 2000
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    i am a student in vienna and thinking right now of doing my final =
thesis on the bioacoustics of frogs (tropical). in my institute they are =
using CANARY for analysing the frog calls. but since i don=B4t have a =
mac i cannot use this on my labtop. is there anywhere a pc version of =
this programm?
    sonja amoser
   =20
        I'm not aware of a PC version of CANARY, but you might want to =
look at a spectrogram program downladable from
    http://www.monumental.com/rshorne/examples.html
   =20
    You will also need a sound card and means of connecting a recorder =
to it...
   =20
    JMPylka


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<BLOCKQUOTE=20
style=3D"BORDER-LEFT: #000000 solid 2px; MARGIN-LEFT: 5px; PADDING-LEFT: =
5px">
    <DIV><FONT face=3DArial size=3D2>i am a student in vienna and =
thinking right now=20
    of doing my final thesis on the bioacoustics of frogs (tropical). in =
my=20
    institute they are using CANARY for analysing the frog calls. but =
since i=20
    don&acute;t have a mac i cannot use this on my labtop. is there =
anywhere a=20
    pc version of this programm?</FONT></DIV>
    <DIV><FONT face=3DArial size=3D2>sonja amoser</FONT></DIV>
    <DIV><FONT face=3DArial size=3D2></FONT>&nbsp;</DIV>
    <DIV><FONT color=3D#000000 size=3D2>&nbsp;&nbsp;&nbsp; I'm not aware =
of a PC=20
    version of CANARY, but you might want to look at a spectrogram =
program=20
    downladable from</FONT></DIV>
    <DIV><FONT color=3D#000000 size=3D2></FONT><FONT size=3D2><A=20
    =
href=3D"http://www.monumental.com/rshorne/examples.html">http://www.monum=
ental.com/rshorne/examples.html</A></FONT></DIV>
    <DIV><FONT size=3D2></FONT>&nbsp;</DIV>
    <DIV><FONT color=3D#000000 size=3D2>You will also need a sound card =
and means of=20
    connecting a recorder to it...</FONT></DIV>
    <DIV><FONT color=3D#000000 size=3D2></FONT>&nbsp;</DIV>
    <DIV><FONT size=3D2>JMPylka</FONT></DIV>
    <DIV>&nbsp;</DIV></BLOCKQUOTE></BODY></HTML>

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    i am a student in vienna and thinking right now of doing my final =
thesis on the bioacoustics of frogs (tropical). in my institute they are =
using CANARY for analysing the frog calls. but since i don=B4t have a =
mac i cannot use this on my labtop. is there anywhere a pc version of =
this programm?
    sonja amoser
   =20
        I'm not aware of a PC version of CANARY, but you might want to =
look at a spectrogram program downladable from
    http://www.monumental.com/rshorne/examples.html
   =20
    You will also need a sound card and means of connecting a recorder =
to it...
   =20
    JMPylka


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<DIV>&nbsp;</DIV>
<BLOCKQUOTE=20
style=3D"BORDER-LEFT: #000000 solid 2px; MARGIN-LEFT: 5px; PADDING-LEFT: =
5px">
    <DIV><FONT face=3DArial size=3D2>i am a student in vienna and =
thinking right now=20
    of doing my final thesis on the bioacoustics of frogs (tropical). in =
my=20
    institute they are using CANARY for analysing the frog calls. but =
since i=20
    don&acute;t have a mac i cannot use this on my labtop. is there =
anywhere a=20
    pc version of this programm?</FONT></DIV>
    <DIV><FONT face=3DArial size=3D2>sonja amoser</FONT></DIV>
    <DIV><FONT face=3DArial size=3D2></FONT>&nbsp;</DIV>
    <DIV><FONT color=3D#000000 size=3D2>&nbsp;&nbsp;&nbsp; I'm not aware =
of a PC=20
    version of CANARY, but you might want to look at a spectrogram =
program=20
    downladable from</FONT></DIV>
    <DIV><FONT color=3D#000000 size=3D2></FONT><FONT size=3D2><A=20
    =
href=3D"http://www.monumental.com/rshorne/examples.html">http://www.monum=
ental.com/rshorne/examples.html</A></FONT></DIV>
    <DIV><FONT size=3D2></FONT>&nbsp;</DIV>
    <DIV><FONT color=3D#000000 size=3D2>You will also need a sound card =
and means of=20
    connecting a recorder to it...</FONT></DIV>
    <DIV><FONT color=3D#000000 size=3D2></FONT>&nbsp;</DIV>
    <DIV><FONT size=3D2>JMPylka</FONT></DIV>
    <DIV>&nbsp;</DIV></BLOCKQUOTE></BODY></HTML>

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    i am a student in vienna and thinking right now of doing my final =
thesis on the bioacoustics of frogs (tropical). in my institute they are =
using CANARY for analysing the frog calls. but since i don=B4t have a =
mac i cannot use this on my labtop. is there anywhere a pc version of =
this programm?
    sonja amoser
   =20
        I'm not aware of a PC version of CANARY, but you might want to =
look at a spectrogram program downladable from
    http://www.monumental.com/rshorne/examples.html
   =20
    You will also need a sound card and means of connecting a recorder =
to it...
   =20
    JMPylka


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<BLOCKQUOTE=20
style=3D"BORDER-LEFT: #000000 solid 2px; MARGIN-LEFT: 5px; PADDING-LEFT: =
5px">
    <DIV><FONT face=3DArial size=3D2>i am a student in vienna and =
thinking right now=20
    of doing my final thesis on the bioacoustics of frogs (tropical). in =
my=20
    institute they are using CANARY for analysing the frog calls. but =
since i=20
    don&acute;t have a mac i cannot use this on my labtop. is there =
anywhere a=20
    pc version of this programm?</FONT></DIV>
    <DIV><FONT face=3DArial size=3D2>sonja amoser</FONT></DIV>
    <DIV><FONT face=3DArial size=3D2></FONT>&nbsp;</DIV>
    <DIV><FONT color=3D#000000 size=3D2>&nbsp;&nbsp;&nbsp; I'm not aware =
of a PC=20
    version of CANARY, but you might want to look at a spectrogram =
program=20
    downladable from</FONT></DIV>
    <DIV><FONT color=3D#000000 size=3D2></FONT><FONT size=3D2><A=20
    =
href=3D"http://www.monumental.com/rshorne/examples.html">http://www.monum=
ental.com/rshorne/examples.html</A></FONT></DIV>
    <DIV><FONT size=3D2></FONT>&nbsp;</DIV>
    <DIV><FONT color=3D#000000 size=3D2>You will also need a sound card =
and means of=20
    connecting a recorder to it...</FONT></DIV>
    <DIV><FONT color=3D#000000 size=3D2></FONT>&nbsp;</DIV>
    <DIV><FONT size=3D2>JMPylka</FONT></DIV>
    <DIV>&nbsp;</DIV></BLOCKQUOTE></BODY></HTML>

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From owner-bio-software@hgmp.mrc.ac.uk  Tue Feb  8 03:50:32 2000
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From: phillinj@SLU.EDU (Nancy Phillips)
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Subject: Primers for Mac
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Hi, I am a confirmed Primers fan, and want to take it from a 68030 with a
FPU to a G3/G4. I had an iMac-owning friend take Primers and mount it on
her machine, and she reported "Here is the message I got "expected a
floating point but found something else".  It does give you a script of
commands, perhaps it can be written around by someone who knows how?". Can
you advise me on whether this program can be salvaged with a FPU emulator?
The reason I like it is that I want to be able to scrutinize sequence
closely and position primers with relation to DNA features (away from poor
quality seq., away from feature of importance for mutation testing, etc)
and I like the control one gets with semi-manual design. I work with high
GC sequences and have 95% success in primer design. Thanks for any ideas. I
am not a programmer. 


Nancy Phillips, M.D.			phone:(314)577-8782
Pathology				fax:(314)268-5120
St. Louis University Hospital		email: phillinj@slu.edu
3635 Vista Ave.
St. Louis, MO, 63110, USA

aacgccaattgctatccccatattctgctaatcccgagcatggac
---


From owner-bio-software@hgmp.mrc.ac.uk  Tue Feb  8 09:21:50 2000
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From: letondal@pasteur.fr (Catherine  Letondal)
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In article <m0aeldeccq.fsf@pc2-44.botanik.uni-duesseldorf.de>, Andrea Hansen <webmaster@bioinformatik.de> writes:
>david@barrass1.no.spam.ta (David Barrass) writes:
>
>> Does anyone know of good and free Oligonocleotide probe/PCR primer
>> design software for Windows/DOS?
>> 
>> I've had a look at Oligo5.0 and it looks excellent, but expensive
>Hi,
>try this site:
>http://www.bioinformatik.de/cgi-bin/browse/Catalog/PCR/Primer_Design/
>
>Andrea

You may try this as well:
http://bioweb.pasteur.fr/seqanal/interfaces/primo.html

-- 
Catherine Letondal -- Pasteur Institute Computing Center


From owner-bio-software@hgmp.mrc.ac.uk  Tue Feb  8 10:44:37 2000
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From: Nicolas Le Novere <nl223@cus.cam.ac.uk>
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Subject: Re: Oligonucleotide Design
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David Barrass wrote:
> 
> Does anyone know of good and free Oligonocleotide probe/PCR primer
> design software for Windows/DOS?

The program MELTING does not chose your primer, but it can check 
that your primers have the same thermodynamic behavior (i.e. the
same optimal annealing temperature), which is one of the main cause
of failure I experienced.
As far as I know, the last version is the most accurate of the 
"market" (If I am please tell me). Besides that it is free in both
sense of the term (free of charge and open-source), and can be 
compiled on every operating systems.

The new version can be found at: 
http://www.pasteur.fr/recherche/unites/neubiomol/ meltinghome.html

A WWW server with the previous version:
http://bioweb.pasteur.fr/seqanal/interfaces/melting.html

--
Dr Nicolas Le Novčre                   e-mail: nl223@cus.cam.ac.uk 
Dpt Zoology, Univ Cambridge, Downing street, Cambridge CB2 3EJ, UK

tel: +44 1223 336623                          fax: +44 1223 336676


From owner-bio-software@hgmp.mrc.ac.uk  Tue Feb  8 11:14:40 2000
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From: "Martin Beek" <martin@serverscan.com>
X-Newsgroups: alt.binaries.dominion.shareware,alt.binaries.shareware,alt.binaries.shareware.ibm.pc,alt.comp.shareware,alt.software,bionet.software,bit.software,biz.comp.software
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Shareware submission report of: "Straycat Windows Cleaner"
Category: Win. 95/98 Utilities / Disk management / Disk cleaners

Date: feb. 7 2000
Version: 1.2 ShareWare
Filename: straycat.exe
Filesize: 1458776 bytes
Status: Shareware
Author: M. Beek / martin@serverscan.com

Submitted to:
Tucwows.com/Winfiles.com/Download.com/Shareware.com/WinFiles.com/winsite.com

Download location: http://www.straycat.serverscan.com

Description:
----------------------------------------------------------------------------
--------
StrayCat will scan your hard disks for 100+ known temporary files,
garbage files and temporary directories left behind (all the time) by
the programs you use, and by Windows itself.
StrayCat can be configured to run on specified time intervals to keep
your hard disks in condition and your Windows system performance top
notch. Straycat is configurable from both the program menu and the
Windows Control Panel.
It can be updated with a language module, so all menu commands and
program response will be displayed in your language. Straycat is a
program that 'hides' in the Windows System Tray, from where it's menu
can be accessed.

Download location: http://www.straycat.serverscan.com

--
Regards,

Martin Beek
--------------------





From owner-bio-software@hgmp.mrc.ac.uk  Tue Feb  8 21:59:51 2000
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Subject: Softwares for good prices
From: ugnyte@takas.lt
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Date: 8 Feb 2000 23:55:06 +0200
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You need wares - no problem :

Newest wares  for good price: 

Autodesk Architektural Desktop Full, Virtual gibbs 5.1, Solid edge 7, Cimatron 10.5, Powermill 2.475, GES, v3.33, Mastercam 7.2B, Designspace 5.0, Autocad 2000 with tutorial, Maya 2.5 + tutorial on 2 CD's, Nastran 70.5.5, MSC Nastran for Windows 4.0, Patran v8.5, Imageware RPM 9.0C,Verdict 9.0C, Surfacer 9.0C, Softimage 3.8 SP2 FOR WIN NT, sumatra beta1, Think3 design eureka 99, Cosmos work 5, Solidwork 1999 + WIN 9x / NT, Pro engineer 2000i with help, Pro mechanica 2000i, Designcad Pro 2000, Catia 5 r2 with tutorial, Helix design 99, Vectorworks minicad 8.0 (cracked), 3D studio MAX 3 release, Unigraphics 15.02, Artcam pro 4, Autodesk mechanical desktop 4, I-Deas 7 5 CD set, C-mold quickfill 99.7, Solid View RP Master ...

... and more others !!! 

Please write to:  saran@prontomail.com ; markstudent@england.com ;  wirgisam-wolf@prontomail.com 

fax: (0870)133-4048
ICQ: 57092777

 




From owner-bio-software@hgmp.mrc.ac.uk  Wed Feb  9 12:29:59 2000
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From: Nicolas Le Novere <nl223@cus.cam.ac.uk>
X-Newsgroups: fr.bio.logiciel,bionet.software
Subject: release of MELTING4
Date: Wed, 09 Feb 2000 11:53:35 +0000
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Release of MELTING4:
-------------------

NAME
       melting  -  nearest-neighbor  computation  of nucleic acid
       hybridation

SYNOPSIS
       melting [options]

DESCRIPTION
       Melting computes, for a nucleic acid duplex, the  enthalpy
       and the entropy of the helix-coil transition, and then its
       melting temperature.  Three  types  of  hybridisation  are
       possible: DNA/DNA, DNA/RNA, and RNA/RNA.  The program uses
       the method of nearest-neighbors. The set of  thermodynamic
       parameters  can  be easely changed, for instance following
       an experimental breakthrough. Melting is a free program in
       both  sense  of  the term. It comes with no cost and it is
       open-source. In addition it is coded in ISO C and  can  be
       compiled  on  any  operating  system. Some perl script are
       provided to show how melting can be used  as  a  block  to
       construct more ambitious programs.

OPTIONS
[snips]

ALGORITHM
   Thermodynamics of helix-coil transition of nucleic acid
       The nearest-neighbor approach is based on  the  fact  that
       the  helix-coil  transition  works  as a zipper.  After an
       initial attachment, the  hybridisation  propagates  later­
       ally.   Therefore,  the  process  depends  on the adjacent
       nucleotides on  each  strand  (the  Crick's  pairs).   Two
       duplexes  with  the  same  base pairs could have different
       stabilities, and on the contrary, two duplexes  with  dif­
       ferent  sequences but identical sets of Crick's pairs will
       have the same thermodynamics properties

FILES
[snips]
       melting.ptk
              A Graphical User Interface written  in  perl/tk  is
              available  for  those  who  prefer  the 'button and
              menu' approach.
[snips]

SEE ALSO
       New  versions  and  related  material  can  be  found   at
       http://www.pasteur.fr/recherche/unites/neubiomol/melt­
       inghome.html

       You  can   use   MELTING   through   a   web   server   at
       http://bioweb.pasteur.fr/seqanal/interfaces/melting.html

COPYRIGHT
       Melting is copyright (C) 1997, 2000 by Nicolas Le Novčre

       This  program  is  free  software; you can redistribute it
       and/or modify it under the terms of the GNU General Public
       License  as  published  by  the  Free Software Foundation;
       either version 2 of the License, or (at your  option)  any
       later version.
[snips]

AUTHOR
       Nicolas  Le  Novčre,  Department of Zoology, University of
       Cambridge  Downing   street,   CB2   3EJ   Cambridge,   UK
       nl223@cus.cam.ac.uk


--
Dr Nicolas Le Novčre                   e-mail: nl223@cus.cam.ac.uk 
Dpt Zoology, Univ Cambridge, Downing street, Cambridge CB2 3EJ, UK
http://www.pasteur.fr/recherche/unites/neubiomol/ 
tel: +44 1223 336623                          fax: +44 1223 336676


From owner-bio-software@hgmp.mrc.ac.uk  Wed Feb  9 14:16:38 2000
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Date: Wed, 09 Feb 2000 16:07:47 +0200
From: Win Hide <winhide@sanbi.ac.za>
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We are developing a system with PHD in mind. Anyone out there with 
a list of command line inputs for PHD?

Win


From owner-bio-software@hgmp.mrc.ac.uk  Wed Feb  9 14:54:22 2000
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Looking for Software? See http://home.wanadoo.nl/~home2000





From owner-bio-software@hgmp.mrc.ac.uk  Wed Feb  9 18:31:37 2000
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From: "Martin Huseby Karlsen" <martinhu@stud.ntnu.no>
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Subject: downloading problems/ program to reduce/limit my dl speed
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I'm on a pretty fast connection, and sometimes when i download i goes too
fast for my networkcard or hard drive. Is there a program that can help me
to reduce the speed, I know FTP-server programs can set a speed limit. can
anyone help me getting a program that can let me control my incomming speed?

mhk





From owner-bio-software@hgmp.mrc.ac.uk  Wed Feb  9 22:35:09 2000
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From: "S.W. Rasmussen" <swr@crc.dk>
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Subject: DNATools revision 662
Date: Wed, 09 Feb 2000 23:26:01 +0000
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Dear DNATools user,

The latest addition to DNATools is a function for sequence comparison using a
dot matrix method. Look under the Search menu. The SAGE functions have been
carefully tested after a major revision and now seems to to work quite well.
Several minor bugs have been fixed. 

A major update of the program manual on the homepage is in progress. You can
download the program - and look at about half of the manual at: 

http://www.crc.dk/phys/dnatools.htm

The search function on the homepage is useful for locating information on
specific functions.

Regards

Soeren

-- 
Soeren W. Rasmussen, Dr. scient.
Department of Physiology
Carlsberg Laboratory
Copenhagen, Denmark
swr@crc.dk, http://www.crc.dk/phys


From owner-bio-software@hgmp.mrc.ac.uk  Fri Feb 11 01:06:49 2000
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From: szia@hanga.enzim.hu (Andras Szilagyi)
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Subject: E-mail address of Anthony Nicholls?
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Hi,

does anybody know the current e-mail address of Anthony Nicholls, the
author of GRASP? GRASP is a software for coloring protein surfaces by
some properties, most often the electrostatic potential. Anthony Nicholls
used to be in Barry Honig's lab at Columbia University but he is
apparently not there any longer. If you know his current e-mail address
then please e-mail me.

Andras Szilagyi, PhD (szia@enzim.hu)
Institute of Enzymology, Hungarian Academy of Sciences
Karolina ut 29., H-1113 Budapest, HUNGARY
Fax: +36 1 466 5465, phone: +36 1 466 5633


From owner-bio-software@hgmp.mrc.ac.uk  Fri Feb 11 07:16:51 2000
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Reply-To: "SoftWarez Cafe" <uik87g@writemail.com>
From: "SoftWarez Cafe" <uik87g@writemail.com>
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Subject: Latest SoftWare For P.C. & Apple Mac
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Send an email with "THE LIST" in the subject line to

auto.program.list@iname.com  Or  uik87g@writemail.com

"SoftWare For P.C, Apple Macintosh,  Playstation & MP3 Music"

...And we will send you a Full List with just about any Software package
that's going.
The software's supplied "strictly for backup purposes only"




From owner-bio-software@hgmp.mrc.ac.uk  Fri Feb 11 20:49:31 2000
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Hi!
i have two questions!!
I have a Windows NT 4.0 OS. The hard drive is 20 GB but in Disk
administrator it shows
8056 MB. And in the partition section its partioned as around ~4 GB as C:
active partition and F: for the rest ~17 GB. Both are the primary partition.

1) How is that so??

Secondly, i deleted the F: drive (~17 gigs) becoz of i wanted to
repartition it differently. Now the whole partition section in Disk
administrator shows the free space left as ~4 gb and the C: parttion .

Where the rest of it is sitting and how can I access it.

Thanks in advance.!!


  

---


From owner-bio-software@hgmp.mrc.ac.uk  Sat Feb 12 00:01:01 2000
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From: karplus@cse.ucsc.edu (Kevin Karplus)
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In article <38980088.D5C4C3EF@cus.cam.ac.uk>, Nicolas Le Novere <nl223@cus.cam.ac.uk> writes:
|> Timour Ivashchenko wrote:
|> 
|> > Can anybody tell me where to get free software which can
|> > calculate the following parameters
|> > based on amino acid sequences:
|> > 1. Kyte&Doolittle Hydrophobicity priofile
|> 
|> Obsolete.
|> 
|> > 2. Protein secondary structure
|> 
|> Review on www.pasteur.fr/recherche/unites/neubiomol/secstrpr.html
|> 

As of CASP-3 (the last blind test of secondary-structure predictors
two years ago), the best secondary structure predictor was PSIPRED.

http://globin.bio.warwick.ac.uk/psipred/

We hope to beat them this year (we were second best at CASP3) with our
latest predictor:  sam-t99

http://www.cse.ucsc.edu/research/compbio/HMM-apps/T99-query.html

-- 
Kevin Karplus 	karplus@cse.ucsc.edu	http://www.cse.ucsc.edu/~karplus
life member (LAB, Adventure Cycling, American Youth Hostels)
Effective Cycling Instructor #218-ck
Anything below this line is junk added by others without my approval.


From owner-bio-software@hgmp.mrc.ac.uk  Sat Feb 12 05:58:46 2000
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From: "JoeSmith" <Jsmith@earthlink.com>
Subject: Charting Software For Windows
Date: Fri, 11 Feb 2000 23:50:23 -0600
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From owner-bio-software@hgmp.mrc.ac.uk  Sat Feb 12 15:50:20 2000
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I am looking for a good multivariate statistics software package that is
reasonably priced $500 CDN or so.  I have found a program called PC-ORD.
Does anyone have any comments about this program.

Cheers
dale

--
____________
Dale Parker
Homepage
http://www3.sk.sympatico.ca/parkerdw/
Saskatchewan Aquatic Insect Web Page
http://www.usask.ca/biology/skabugs/index.html




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