From owner-srs@net.bio.net Mon Jan 05 22:00:00 1998
From: luca.toldo@merck.de
To: etzold@ebi.ac.uk,verde@ebi.ac.uk
Subject: Re: SRS version 5.1.0
Date: Tue, 06 Jan 1998 07:21:20 -0600
Message-ID: <884091913.96403466@dejanews.com>
Newsgroups: bionet.software.srs
Organization: Deja News Posting Service
Path: biosci!agate!logbridge.uoregon.edu!nntp2.dejanews.com!grunt.dejanews.com!not-for-mail
References: <349F1559.F7B77CE2@ebi.ac.uk>
X-Article-Creation-Date: Tue Jan 06 13:05:14 1998 GMT
X-Authenticated-Sender: luca.toldo@merck.de
X-Http-User-Agent: Mozilla/4.04 [en] (WinNT; I)  via proxy gateway  CERN-HTTPD/3.0 libwww/2.17
X-Originating-IP-Addr: 155.250.128.1 (wingate.merck.de)
Lines: 23

In article <349F1559.F7B77CE2@ebi.ac.uk>,
  Thure Etzold <etzold@ebi.ac.uk> wrote:
>
> Dear all,
>
> happy Christmas and lots of new features. Have fun!

The best wishes of happy Christmas and happy new year 1998 to Thure,
Giorgio and the entire SRS team at EBI, which is providing the Scientific
Community with such a powerfull tool as SRS !

Best wishes to SRS5.1 to keep up with its preceding versions, and wishes
for its outcomes !

Compliments again

Dr. Luca Ida Giovanni TOLDO (Ph.D.)
MERCK KGaA
Darmstadt
Germany

-------------------==== Posted via Deja News ====-----------------------
      http://www.dejanews.com/     Search, Read, Post to Usenet

From owner-srs@net.bio.net Tue Jan 06 22:00:00 1998
From: luca.toldo@merck.de
To: etzold@ebi.ac.uk
Subject: Q&A: How to install SRS5.1.0 on a Netscape server
Date: Wed, 07 Jan 1998 02:51:11 -0600
Message-ID: <884162458.1130168322@dejanews.com>
Newsgroups: bionet.software.srs
Organization: Deja News Posting Service
Path: biosci!agate!howland.erols.net!news.maxwell.syr.edu!nntp2.dejanews.com!grunt.dejanews.com!not-for-mail
X-Article-Creation-Date: Wed Jan 07 08:40:58 1998 GMT
X-Authenticated-Sender: luca.toldo@merck.de
X-Http-User-Agent: Mozilla/4.04 [en] (WinNT; I)  via proxy gateway  CERN-HTTPD/3.0 libwww/2.17
X-Originating-IP-Addr: 155.250.128.1 (wingate.merck.de)
Lines: 70

It happens that not everybody is running the same version of the httpd,
and it happens as well that Netscape Enterprise Server is a rather common
httpd. What follows is a
"how-to-install-srs-on-netscape-enterprise-server" that I communicated on
the 23.04.97 to the SRS developers but that never made it into the
official distribution since for what I understand they do not use the
Netscape httpd server and therefore could not easily test it on their
side.

Please, notice that the following information is provided on an AS-IS
basis, and you agree in using this information at your own risk (:->).

1) in obj.conf

          a) include the following object: (example below done
                                            assuming the full path
                                            to the home page
be /usr/local/htdocs)

<Object ppath="/usr/local/htdocs/srs5bin/cgi-bin/*">
ObjectType fn="force-type" type="magnus-internal-cgi"
Service fn="send-cgi"
</object>

          b) include in the _default_ Object: (example below done
                                            assuming the full path
                                            to the srs install be
                                            /home/srs)

NameTrans from="/srs5/" fn="pfx2dir" dir="/home/srs/srs/www"

2) place in /usr/local/htdocs the link to the /home/srs/srs/www and call
   it srs5:

%ln -s /home/srs/srs/www /usr/local/htdocs/srs5

3) for matter of practice I then did the following but perhaps could be
   done also in a "cleaner" way:

a) mkdir /usr/local/htdocs/srs5bin/cgi-bin
b) cp /home/srs/srs/www/bin/wgetz /usr/local/htdocs/srs5bin/cgi-bin/wgetz


Once those modifications to the obj.conf are manually done (unfortunately
this process cannot be performed through the web interface to the
Netscape server), then send a hangup to the server and restart it (can as
well use the web interface, reload the config files stop and start the
server).


Considerations
--------------

Using the above mechanism, I maintain several versions of the SRS system
running on the same machine and simply have each of them in a different
directory e.g. /home/srs/srs5.0.4 /home/srs/srs5.0.5 /home/srs/srs5.1.0
  etc.. and the entire setup is pretty simple to manage ...


Perhaps this information could be included in the SRS FAQ and/or in the
message returned upon completion of %srsinstall www

I do not consider this contribution as specially crucial but still, could
save some time specially to new srs maintainers ...

Regards, Compliments, and happy new year
Dr. Luca I.G. TOLDO

-------------------==== Posted via Deja News ====-----------------------
      http://www.dejanews.com/     Search, Read, Post to Usenet

From owner-srs@net.bio.net Tue Jan 06 22:00:00 1998
From: luca.toldo@merck.de
To: etzold@ebi.ac.uk
Subject: Q: Performance of SRS5.1.0
Date: Wed, 07 Jan 1998 04:01:11 -0600
Message-ID: <884166646.1201471495@dejanews.com>
Newsgroups: bionet.software.srs
Organization: Deja News Posting Service
Path: biosci!daresbury!server5.netnews.ja.net!nntp.news.xara.net!xara.net!news.maxwell.syr.edu!nntp2.dejanews.com!grunt.dejanews.com!not-for-mail
X-Article-Creation-Date: Wed Jan 07 09:50:46 1998 GMT
X-Authenticated-Sender: luca.toldo@merck.de
X-Http-User-Agent: Mozilla/4.04 [en] (WinNT; I)  via proxy gateway  CERN-HTTPD/3.0 libwww/2.17
X-Originating-IP-Addr: 155.250.128.1 (wingate.merck.de)
Lines: 62

Dear Colleagues, I was just wondering if benchmarks on the performance of
the srsbuild process were available or were foreseen to be exploited.  I
agree that since the srsbuild is relying on the icarus scripts which can
be changed at any site appropriately, at least theoretically it could be
troublesome to compare indexing performance.  Moreover, since the
indexing time is certainly dependent upon the size and the format of the
database used there are various parameters to be taken into
consideration.	Certainly wether the data and index disks are local or
remotely mounted that as well can make a difference .. as well as the
machine load etc...  Finally, since the index generation process is
composed of several steps, the choice of which of the srsbuild atomic
operations is also a variable.


However, I think that is useful know quantitatively about the performance
of the srsbuild step and its monitoring on wide range of platforms.


Hereafter I provide an example of a record which one may consider as
prototype for this kind of data-collection ... restricting to the very
first srsbuild command launched, the one which is launched with the
parameters: srsbuild XXXXX -xdir XXX: -odir XXX: -s unix -nn

---------------------------

A) Hardware parameters
Processor type:
CPU speed:
data location:
index location:

B) Operating System information
Operating System Name:
Operating System Version:

C) Database specific information
Database:
Format:
DBRelease:
SRSRelease:

D) Performance
real cpu time:
user cpu time:
sys  cpu time:

------------------------

 Since certain Organizations could feel that this data contain
proprietary information, the report could be then "anonymized" by the SRS
maintainers, which each of us trust fully.

 This is just a suggestion, which hope will stimulate discussion and
actions on this important aspect, which fits into the general area of
performance in biocomputing...


regards and compliments
Dr. Luca I.G. TOLDO

-------------------==== Posted via Deja News ====-----------------------
      http://www.dejanews.com/     Search, Read, Post to Usenet

From owner-srs@net.bio.net Thu Jan 08 22:00:00 1998
From: luca.toldo@merck.de
To: etzold@ebi.ac.uk
Subject: BUG+ Q.&A.: How to build offline indexes with SRS 5.1.0
Date: Fri, 09 Jan 1998 09:01:17 -0600
Message-ID: <884357601.1744961551@dejanews.com>
Newsgroups: bionet.software.srs
Organization: Deja News Posting Service
Path: biosci!daresbury!uninett.no!news.algonet.se!newsfeed.direct.ca!logbridge.uoregon.edu!nntp2.dejanews.com!grunt.dejanews.com!not-for-mail
X-Article-Creation-Date: Fri Jan 09 14:53:21 1998 GMT
X-Authenticated-Sender: luca.toldo@merck.de
X-Http-User-Agent: Mozilla/4.04 [en] (WinNT; I)  via proxy gateway  CERN-HTTPD/3.0 libwww/2.17
X-Originating-IP-Addr: 155.250.128.1 (wingate.merck.de)
Lines: 53

Dear SRS bionet-ters, I hope each of you had already time to investigate
on the great contents of the latest version of SRS 5.1.0 ! Really a nice
piece of work what the brilliant Team coordinated by Thure Etzold did
recently. We should all be grateful to them for their really useful tool
! Hereafter I would like to inform you of a really minor inconvenient
which I just encountered right now while exploring for the first time the
-off flag in srscheck and srsupdate .

You are perfectly aware that with the version 5.1.0 is possible to have
offline index generation so that one could build indexes for different
versions of the flat files and then move the flat file and the indexes at
the same time to the new location. This is a really GREAT possibility
that the SRS team did since infact it was a current problem when large
database updates where happening ...

Now, the problem is that after one has generated the appropriate srsupdate
with the command

%srscheck -off

then launching the srsupdate will result in a SRSICA error "error: file
could not be opened, "SRSOFFINX:..." followed by a bunch of srsupdate
core dumps (exactely one for each invokation of srsbuild in the srsupdate
script minus 1).

The reason for that is that there is an inconsistency between the content
of the environment variable SRSOFFINX  and what is distributed in the
srs5.1.0 tar file.

SRSOFFINX points to $SRSROOT/offindex but the tar archive instead upon
untarring will generate the indexOff directory instead.

The problem is very easily solved, therefore, by simply this command:

mv $SRSROOT/indexOff $SRSROOT/offindex

Infact, I have the feeling that the SRS developers _do_ want the
directory be called offindex since it appears specifically both in each
of the scripts:

$SRSROOT/etc/templ-prep_srs
$SRSROOT/etc/templ-prep_srs.sh
$SRSROOT/etc/prep_srs
$SRSROOT/etc/prep_srs.sh

Perhaps in the next release of the SRS5.1.0 tar the SRS developers will
include this change by default...

Regards and good SRS-sing !
Dr. Luca Ida Giovanni TOLDO (Ph.D.)

-------------------==== Posted via Deja News ====-----------------------
      http://www.dejanews.com/     Search, Read, Post to Usenet

From owner-srs@net.bio.net Thu Jan 08 22:00:00 1998
From: luca.toldo@merck.de
To: etzold@ebi.ac.uk
Subject: Q.&A.:How To load proteinChart from the local Server 
Date: Fri, 09 Jan 1998 09:52:12 -0600
Message-ID: <884361014.1943797762@dejanews.com>
Newsgroups: bionet.software.srs
Organization: Deja News Posting Service
Path: biosci!agate!arclight.uoregon.edu!logbridge.uoregon.edu!nntp2.dejanews.com!grunt.dejanews.com!not-for-mail
X-Article-Creation-Date: Fri Jan 09 15:50:14 1998 GMT
X-Authenticated-Sender: luca.toldo@merck.de
X-Http-User-Agent: Mozilla/4.04 [en] (WinNT; I)  via proxy gateway  CERN-HTTPD/3.0 libwww/2.17
X-Originating-IP-Addr: 155.250.128.1 (wingate.merck.de)
Lines: 25

Dear Colleagues, A small tip when installing SRS5.1.0 and want to provide
your users with the fastest feed with the marvellous proteinChart applet:

1.modify srsgen.i :
in the codebase replace the call to ebi with your own machine and path ...

2. srssection

In that way, when the applet is requested its classes (perhaps in the
future a single jar ?) are downloaded from your server and not "across
the world" and this could /should provide your users with a faster
feedback (specially if your connection to internet is not very high).

I would like to stress the point that the applet integration how had been
reimplemented in version 5.1.0 is really GREAT since does not require any
longer modifying the C source code and allows a much greater possibility
to add applet viewers to the results of queries.

My compliments for the excellent work done in Dr. Etzold's group !
regards

Dr. Luca Ida Giovanni TOLDO (Ph.D.)

-------------------==== Posted via Deja News ====-----------------------
      http://www.dejanews.com/     Search, Read, Post to Usenet

From owner-srs@net.bio.net Thu Jan 08 22:00:00 1998
From: luca.toldo@merck.de
To: etzold@ebi.ac.uk
Subject: BUG? srsupd.pl in version 5.1.0
Date: Fri, 09 Jan 1998 10:11:43 -0600
Message-ID: <884361966.1958346761@dejanews.com>
Newsgroups: bionet.software.srs
Organization: Deja News Posting Service
Path: biosci!daresbury!uninett.no!Cabal.CESspool!bofh.vszbr.cz!news.maxwell.syr.edu!nntp2.dejanews.com!grunt.dejanews.com!not-for-mail
X-Article-Creation-Date: Fri Jan 09 16:06:06 1998 GMT
X-Authenticated-Sender: luca.toldo@merck.de
X-Http-User-Agent: Mozilla/4.04 [en] (WinNT; I)  via proxy gateway  CERN-HTTPD/3.0 libwww/2.17
X-Originating-IP-Addr: 155.250.128.1 (wingate.merck.de)
Lines: 17

Dear SRS managers, I have just noticed that etc/srsupd.pl distributed
with srs5.1.0 contains also the following lines in addition to the ones
in previous version srs5.0.5:

$com =
      "srscheck -L pdbreport -o $srsupd; " .
      "$srsupd";
system ($com");

I was just wondering and I think that perhaps are a left-over from a local
version of those scripts by the SRS developers ?

Regards
Dr. Luca Ida Giovanni TOLDO (Ph.D.)

-------------------==== Posted via Deja News ====-----------------------
      http://www.dejanews.com/     Search, Read, Post to Usenet

From owner-srs@net.bio.net Thu Jan 08 22:00:00 1998
Path: biosci!agate!newsfeed.kornet.nm.kr!nntp.kreonet.re.kr!news.maxwell.syr.edu!Cabal.CESspool!bofh.vszbr.cz!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!not-for-mail
From: Thure Etzold <etzold@ebi.ac.uk>
Newsgroups: bionet.software.srs
Subject: revised srs5.1.0
Date: Fri, 09 Jan 1998 16:49:25 +0000
Organization: MRC Human Genome Mapping Project Resource Centre
Lines: 30
Message-ID: <34B65514.CC8FC09A@ebi.ac.uk>
NNTP-Posting-Host: o2-3.ebi.ac.uk
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 4.03 [en] (X11; I; IRIX 6.3 IP32)

dear all,

a few problems have been reported with version 5.1 that i have now
fixed. I replaced the srs5.1.0.tar.gz with the new version
file rather than making yet another release.

The following problems have been solved:

o The directory indexOff was renamed to offindex.
o The tar file includes all documentation.
o On some platforms the offline index directory was used as
  default.
o OSF has a very low limit for the stack size, Solaris a low limit.
  on file descriptors which both affect indexing of large databanks.
  I included an unlimit command for stacksize and
  descriptors in the script generated by srscheck.
o Merging very large indices crashed because of an allocation limit
o Very large sequences could not be submitted because of an allocation
  limit.
o TRANSFAC *.i files have been updated to the version at
  GBF/Braunschweig.
o ...and some more mysterious bugs.


many thanks for all who sent me error reports!

regards
Thure



From owner-srs@net.bio.net Sat Jan 10 22:00:00 1998
Path: biosci!agate!newsfeed.kornet.nm.kr!nntp.kreonet.re.kr!news.maxwell.syr.edu!Supernews60!supernews.com!Supernews69!not-for-mail
From: "joedapro" <joedapro@loop.com>
Newsgroups: bionet.software.srs
Subject: [[[ ADVERTISE YOUR BUSINESS AND MAKE MONEY $$$ ]]]
Date: Sat, 10 Jan 1998 21:40:15 -0800
Organization: All USENET -- http://www.Supernews.com
Lines: 34
Message-ID: <699l7g$gap$1@usenet87.supernews.com>
NNTP-Posting-Host: 16729@207.211.62.211
X-Newsreader: Microsoft Outlook Express 4.72.2106.4
X-MimeOLE: Produced By Microsoft MimeOLE V4.72.2106.4

The PC-SUPER-ADVERTISER system is the most simple, powerful
and affordable Money Making program in the world!

$$$$$ It costs only $ 6 USD one time! $$$$$
-------------------------------

But wait ... before you pay, check out these features:

- Advertising Space is our PRODUCT.
You buy Advertising Space, then you USE it to advertise
your business or idea. Then you RE-SELL this Advertising
Space hundreds or thousands of times!

- MULTI-LEVEL MARKETING gives you the potential to reach
more people than you personally could! You could earn
LOTS OF MONEY in residual income re-selling the Ad-Space!

- Use your Computer and the INTERNET to Duplicate,
Advertise and Distribute our Software totally "on-line"
to anywhere in the WORLD with ZERO COST!

- The program runs under DOS or Windows. Very easy to use.


Download your FREE copy of PC-SUPER-ADVERTISER now, study the
business, see the potential, even play a FREE game included!

Now you can ADVERTISE at a very low cost and MAKE MONEY
at the same time! Have a super day!

Please contact Joe at:  joedapro@hotmail.com     Subject:  PSA




From owner-srs@net.bio.net Sat Jan 10 22:00:00 1998
Path: biosci!newshost.lanl.gov!awabi.library.ucla.edu!128.230.129.106!news.maxwell.syr.edu!Supernews60!supernews.com!Supernews69!not-for-mail
From: "joedapro" <joedapro@loop.com>
Newsgroups: bionet.software.srs
Subject: [[[   Become a Software Distributor !   ]]]
Date: Sat, 10 Jan 1998 21:42:11 -0800
Organization: All USENET -- http://www.Supernews.com
Lines: 17
Message-ID: <699l98$dov$1@usenet76.supernews.com>
NNTP-Posting-Host: 14111@207.211.62.211
X-Newsreader: Microsoft Outlook Express 4.72.2106.4
X-MimeOLE: Produced By Microsoft MimeOLE V4.72.2106.4

Work from home with your PC distributing software and
     information all over the world via E-mail and the Internet.

     Our exclusive software PC-SUPER-MLM will give you all the
     information and tools you need to start your own Computer
     Based Business as a Software Distributor.

     Make thousands of dollars doing simple and pleasant work
     from home with your computer using our proven methods.

     Reply to this article and I will send you a FREE copy of
     the PC-SUPER-MLM software for your evaluation.

Please contact Joe at:  joedapro@hotmail.com    Subject:  PSM




From owner-srs@net.bio.net Sun Jan 11 22:00:00 1998
From: luca.toldo@merck.de
To: etzold@ebi.ac.uk,verde@ebi.ac.uk
Subject: Re: Q.&A.:How To load proteinChart from the local Server 
Date: Mon, 12 Jan 1998 07:32:24 -0600
Message-ID: <884611493.1115553796@dejanews.com>
Newsgroups: bionet.software.srs
Organization: Deja News Posting Service
Path: biosci!daresbury!uninett.no!newscore.univie.ac.at!news-raspail.gip.net!news-peer.gip.net!news.gsl.net!gip.net!news.maxwell.syr.edu!nntp2.dejanews.com!grunt.dejanews.com!not-for-mail
References: <884361014.1943797762@dejanews.com>
X-Article-Creation-Date: Mon Jan 12 13:24:54 1998 GMT
X-Authenticated-Sender: luca.toldo@merck.de
X-Http-User-Agent: Mozilla/4.04 [en] (WinNT; I)  via proxy gateway  CERN-HTTPD/3.0 libwww/2.17
X-Originating-IP-Addr: 155.250.128.1 (wingate.merck.de)
Lines: 53

Dear Colleagues,  in the new revision of srs5.1.0 that Dr. Etzold
deposited, there is PLENTY of  applet viewers ! That is really a great
added value that Dr. Etzold and Giorgio  Verde did recently and not very
much appreciated yet by the SRS users ! We must  be very grateful for the
excellent work that Team is exploiting in EBI !

 Here I would like just to suggest you a trick I find great: instead of
placing  all those single .class's that then soon or later will clash
between different  codes, pack up in a JAR all the classes needed and
then use the archive command  in the applet call. The speedup also in
downloading is NOTICEABLE and the  maintenance of your www server much
improved !

 Here is what I did, building the JAR using JDK1.1.5 on a Sun, and
testing the applet successfully using as browser Netscape  4.0.4 on
Windows NT:

 1) Pack all the classes of an applet in a single compressed archive
    (in this example I built the jar for all the classes needed by
     AlignChartApplet)

 jar -cfM AlignChartApplet.jar *.class

 2) Move the jar in the location where one wants to be fetched from, in
this  case I just place it in the $SRSWWW/java directory for sake of
simplicity

 3) Modify the icarus code so that it looks like this:
           |<applet
           |  code="AlignChartApplet.class"
           |  width=600 height=400
           |  codebase="http://your.srs.server/fullpath/java/">
           |  archive="AlignChartApplet.jar"
           |<param name=seq value="
           |($tl.first.obj.str:fasta)
           |">
           |</applet>
    please notice that the "fullpath" should be replaced by the fullpath
    you have set for the srs server... obviously !

 4) srssection


N.B. JAR are a feature of JDK1.1 and thus as long as a browser is JDK1.1
     compliant then this feature as well should be supported.

Hope this help, and perhaps could be included in the next SRS 5.1.X
distrib ?

Dr. Luca Ida Giovanni TOLDO (Ph.D.)

-------------------==== Posted via Deja News ====-----------------------
      http://www.dejanews.com/     Search, Read, Post to Usenet

From owner-srs@net.bio.net Mon Jan 12 22:00:00 1998
From: luca.toldo@merck.de
To: etzold@ebi.ac.uk,verde@ebi.ac.uk
Subject: SRS5.1.0BUG? Q: Core dump when getz -vf ?
Date: Tue, 13 Jan 1998 04:11:41 -0600
Message-ID: <884685990.1818165255@dejanews.com>
Newsgroups: bionet.software.srs
Organization: Deja News Posting Service
Path: biosci!agate!arclight.uoregon.edu!news-xfer.netaxs.com!news.maxwell.syr.edu!nntp2.dejanews.com!grunt.dejanews.com!not-for-mail
X-Article-Creation-Date: Tue Jan 13 10:06:30 1998 GMT
X-Authenticated-Sender: luca.toldo@merck.de
X-Http-User-Agent: Mozilla/4.04 [en] (WinNT; I)  via proxy gateway  CERN-HTTPD/3.0 libwww/2.17
X-Originating-IP-Addr: 155.250.128.1 (wingate.merck.de)
Lines: 18

Dear Bionet SRS mainainters,
I was just wondering if you also are observing on IRIX 6.4 that SRS 5.1.0
getz produces a core dump when invoked with the following statement:

getz '[swissprot-id:100k_RAT]' -vf cit

The same command is not failing when operating on version 5.0.5

The swissprot indexes are fully working and operational otherwhise as
could be checked by the wgetz properly working ...

any feedback is welcome !

regards
Dr. Luca Ida Giovanni TOLDO (Ph.D.)

-------------------==== Posted via Deja News ====-----------------------
      http://www.dejanews.com/     Search, Read, Post to Usenet

From owner-srs@net.bio.net Tue Jan 13 22:00:00 1998
From: Tim Dudgeon <dudgeon@britbio.co.uk>
Subject: Re: blast on srs5.1
Date: Wed, 14 Jan 1998 11:33:29 -0600
Message-ID: <884798805.425590801@dejanews.com>
Newsgroups: bionet.software.srs
Organization: British Biotech
Path: biosci!agate!arclight.uoregon.edu!logbridge.uoregon.edu!nntp2.dejanews.com!grunt.dejanews.com!not-for-mail
X-Article-Creation-Date: Wed Jan 14 17:26:45 1998 GMT
X-Authenticated-Sender: Tim Dudgeon <dudgeon@britbio.co.uk>
X-Http-User-Agent: Mozilla/4.04 [en] (Win95; I)  via proxy gateway  CERN-HTTPD/3.0 libwww/2.17
X-Originating-IP-Addr: 193.130.179.2 (gateway.britbio.co.uk)
Lines: 32

I've come across the solution to a previously posted message about blast
output not being correctly parsed.

I think the reason is that in SRS5.1 the blast output is now expected to
be in the format that is derived from GCG's TOBLAST program rather than
NCBI's *2fasta and setdb programs.

The NCBI programs produce alignment headers that look like

>SW|acc|id des

whilst toblast generated headers like this

>SW:id acc des

where acc is the accession number, id is the entry id and des is the
description line.

So you either need to switch to using the GCG program to generate the
blast databases, or to modify blast.is to read the NCBI generated data.

Hope this helps.

Tim

--
Dr. Tim Dudgeon				Phone: 01865 748747
British Biotech Pharmaceuticals Plc.	FAX:   01865 717598
Watlington Road, Oxford, OX4 5LY, UK	email: dudgeon@britbio.co.uk

-------------------==== Posted via Deja News ====-----------------------
      http://www.dejanews.com/     Search, Read, Post to Usenet

From owner-srs@net.bio.net Tue Jan 13 22:00:00 1998
From: Tim Dudgeon <dudgeon@britbio.co.uk>
Subject: Q&A: SRS5.1 and applications 
Date: Wed, 14 Jan 1998 11:12:33 -0600
Message-ID: <884797523.420872193@dejanews.com>
Newsgroups: bionet.software.srs
Organization: British Biotech
Path: biosci!agate!arclight.uoregon.edu!logbridge.uoregon.edu!nntp2.dejanews.com!grunt.dejanews.com!not-for-mail
X-Article-Creation-Date: Wed Jan 14 17:05:24 1998 GMT
X-Authenticated-Sender: Tim Dudgeon <dudgeon@britbio.co.uk>
X-Http-User-Agent: Mozilla/4.04 [en] (Win95; I)  via proxy gateway  CERN-HTTPD/3.0 libwww/2.17
X-Originating-IP-Addr: 193.130.179.2 (gateway.britbio.co.uk)
Lines: 41

In the light of previous postings to this newsgroup here is another minor
fix to SRS5.1 that people might find useful.

I've encountered a problem with configuring applications in SRS5.1
whereby you do not get all the applications that are configured in
DNASEQ_DATA:$datatype.applications or PROTSEQ_DATA:$datatype.applications
(defined in srsgen.i).

For instance, if you include BLAST_APP and CLUSTALW_APP as your only
applications you only get BLAST accessible in SRSWWW when you have the
results of a protein database query. The reason is that PROTSEQ_DATA
contains the following:

PROTSEQ_DATA:$DataType:[ProteinSequence
  applications:{@?BLAST_APP @?SW_APP @?FASTA_APP @?CLUSTALW_APP
    @?PPSEARCH_APP
  }
]

and as SW_APP is not defined ($libloc:[@SW_DB dir:"" indexDir:""] is
commented out in srsdb.i) you only get BLAST, as any misssing application
appears to prevent SRS 'reading on' to get the full list. To solve the
problem remove any unused applications, i.e.

PROTSEQ_DATA:$DataType:[ProteinSequence
  applications:{@?BLAST_APP @?CLUSTALW_APP
  }
]


Hope this helps.

Tim

--
Dr. Tim Dudgeon				Phone: 01865 748747
British Biotech Pharmaceuticals Plc.	FAX:   01865 717598
Watlington Road, Oxford, OX4 5LY, UK	email: dudgeon@britbio.co.uk

-------------------==== Posted via Deja News ====-----------------------
      http://www.dejanews.com/     Search, Read, Post to Usenet

From owner-srs@net.bio.net Tue Jan 13 22:00:00 1998
Path: biosci!AMC.UVA.NL!a.h.vankampen
From: a.h.vankampen@AMC.UVA.NL ("A.H. van kampen ", automat)
Newsgroups: bionet.software.srs
Subject: problem with srsbuild
Date: 14 Jan 1998 04:53:00 -0800
Organization: Academic Medical Centre Amsterdam
Lines: 31
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <34BCB4F5.EAB66B14@amc.uva.nl>
NNTP-Posting-Host: net.bio.net

Dear,

After building the database index of EMBL and PIR, the next
command results in a 'segmentation fault':

   time srsbuild -i EMBL PIR -xdir 'SRSINX:' -odir 'SRSINX:' -s 'unix'

Is this a known problem, or  may this error  bedue to  a local
(mis)configuration problem. Building the other links between databases
does not give any problems.

Regards,

Antoine

--
   ---------------------------------------
   | Dr. Antoine H.C. van Kampen         |
   | Academic Medical Center Amsterdam   |
   | Cluster Research Support (ADIV-D01) |
   | Meibergdreef 9                      |
   | 1105 AZ  Amsterdam, the Netherlands |
   -------------------------------------------------|
                | Phone:  ++31-20-5667096           |
                | Fax:    ++31-20-5669020           |
                | Email:  a.h.vankampen@amc.uva.nl  |
                | www:    http://www.amc.uva.nl/    |
                -------------------------------------




From owner-srs@net.bio.net Wed Jan 14 22:00:00 1998
Path: biosci!daresbury!uninett.no!news-feed.ifi.uio.no!recycled.news.erols.com!howland.erols.net!fastnet!uunet!in2.uu.net!munnari.OZ.AU!news.unimelb.edu.au!wehi.edu.au!wehi!janice
From: janice@wehi.edu.au (Janice Coventry, Walter and Eliza Hall Institute)
Newsgroups: bionet.software.srs
Subject: Indexing with SRS5.1.0
Date: 15 Jan 98 15:57:04 +1000
Organization: Walter & Eliza Hall Institute
Lines: 19
Message-ID: <1998Jan15.155704@wehi>
NNTP-Posting-Host: wehiu.wehi.edu.au
X-Trace: wehid.wehi.EDU.AU 884840374 12290 (None) 128.250.252.27
X-Complaints-To: newsmaster@wehi.edu.au

Dear SRS Managers

To upgrade to SRS5.1.0 is it necessary to reindex all your files?
I have been testing some of my databases, when I try
to getz entries from the old indices I have a core dump.

Thanking you in anticipation

Cheers,
Janice
 
================================================================================
Janice Coventry
The Walter and Eliza Hall Institute of Medical Research,
P.O. Royal Melbourne Hospital, Victoria, 3050, Australia.
Phone: International +61-3-9345-2564   FAX: International +61-3-9347-0852
            National 03-9345-2564                   National 03-9347-0852
Email:  janice@wehi.edu.au
===============================================================================

From owner-srs@net.bio.net Wed Jan 14 22:00:00 1998
Path: biosci!bloom-beacon.mit.edu!eru.mt.luth.se!feed1.news.luth.se!luth.se!Cabal.CESspool!bofh.vszbr.cz!news.maxwell.syr.edu!newsfeed.nacamar.de!univ-lyon1.fr!news.ujf-grenoble.fr!ciril.fr!u-strasbg.fr!news
From: pingouin@chouchen.u-strasbg.fr (Francois Jeanmougin)
Newsgroups: bionet.software.srs
Subject: Re: blast on srs5.1
Date: 15 Jan 1998 14:34:07 GMT
Organization: CRC - Universite Louis Pasteur - Strasbourg France
Lines: 32
Message-ID: <69l6ov$h@news.u-strasbg.fr>
References: <884798805.425590801@dejanews.com>
    <69keet$nrt@news.u-strasbg.fr> <34BE13D4.22C685A0@ebi.ac.uk>
Reply-To: jeanmougin@igbmc.u-strasbg.fr
NNTP-Posting-Host: chouchen.u-strasbg.fr
Mime-Version: 1.0
Content-Type: text/plain; charset=iso-8859-1
Content-Transfer-Encoding: 8bit
X-Newsreader: knews 0.9.8

In article <34BE13D4.22C685A0@ebi.ac.uk>,
	Thure Etzold <etzold@ebi.ac.uk> writes:

> The BLAST parser in the SRS distribution is for an older version of BLAST. I
> would be really grateful for suggestions on a syntax that is suitable for
> different
> versions.

	I hope Fred will be able to. Just wait (if you can't, just hack
DBWatcher C code from the current distribution :
	ftp://ftp-igbmc.u-strasbg.fr/pub ). In fact, I can't remember, if
it works for all the Blast versions or only for the new ones. 
Can't check now. It would take less time waiting for Fred.

>  We use here at EBI indeed the GCG tools to generate the databanks
> in
> FASTA format. The parser that reads the title line of each BLAST entry should
> be able to read a variety of formats but there is no way of telling whether
> entry
> name or accession number comes first.

	If you check the format of what you are currently reading? I
think you can use, from both names, the one that share the format
you expect, no? Just an idea...

						François.

-- 
François Jeanmougin     | groupe de bioinformatique / bioinformatics groupe
tel:(+33) 3 88 65 32 71 | IGBMC BP 163 67404 Illkirch France



From owner-srs@net.bio.net Wed Jan 14 22:00:00 1998
Path: biosci!daresbury!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!not-for-mail
From: Thure Etzold <etzold@ebi.ac.uk>
Newsgroups: bionet.software.srs
Subject: Re: blast on srs5.1
Date: Thu, 15 Jan 1998 13:49:09 +0000
Organization: MRC Human Genome Mapping Project Resource Centre
Lines: 26
Message-ID: <34BE13D4.22C685A0@ebi.ac.uk>
References: <884798805.425590801@dejanews.com> <69keet$nrt@news.u-strasbg.fr>
NNTP-Posting-Host: o2-3.ebi.ac.uk
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 4.03 [en] (X11; I; IRIX 6.3 IP32)

Francois Jeanmougin wrote:

> In article <884798805.425590801@dejanews.com>,
>         Tim Dudgeon <dudgeon@britbio.co.uk> writes:
> > I've come across the solution to a previously posted message about blast
> > output not being correctly parsed.
>
>         I think it's not only an expected GCG ouput, it is also because
> different version of blast gives different output. We experienced such
> problems with DBWatcher...
>         If I remember correctly, Fred found a way to parse easily
> both outputs, but, Fred is out this week, so he will confirm next
> week if I'm right.

The BLAST parser in the SRS distribution is for an older version of BLAST. I
would be really grateful for suggestions on a syntax that is suitable for
different
versions.  We use here at EBI indeed the GCG tools to generate the databanks
in
FASTA format. The parser that reads the title line of each BLAST entry should
be able to read a variety of formats but there is no way of telling whether
entry
name or accession number comes first.

Thure


From owner-srs@net.bio.net Wed Jan 14 22:00:00 1998
From: Tim Dudgeon <dudgeon@britbio.co.uk>
Subject: Re: Indexing with SRS5.1.0
Date: Thu, 15 Jan 1998 05:01:56 -0600
Message-ID: <884861929.1079246858@dejanews.com>
Newsgroups: bionet.software.srs
Organization: British Biotech
Path: biosci!daresbury!uninett.no!news.algonet.se!newsfeed.direct.ca!news-peer.sprintlink.net!news.sprintlink.net!Sprint!news.maxwell.syr.edu!nntp2.dejanews.com!grunt.dejanews.com!not-for-mail
References: <1998Jan15.155704@wehi>
X-Article-Creation-Date: Thu Jan 15 10:58:49 1998 GMT
X-Authenticated-Sender: Tim Dudgeon <dudgeon@britbio.co.uk>
X-Http-User-Agent: Mozilla/4.04 [en] (Win95; I)  via proxy gateway  CERN-HTTPD/3.0 libwww/2.17
X-Originating-IP-Addr: 193.130.179.2 (gateway.britbio.co.uk)
Lines: 22

In article <1998Jan15.155704@wehi>,  janice@wehi.edu.au (Janice Coventry,
Walter and Eliza Hall Institute) wrote:

> To upgrade to SRS5.1.0 is it necessary to reindex all your files?
> I have been testing some of my databases, when I try
> to getz entries from the old indices I have a core dump.


Do you need to reindex? Almost certainly yes. Quite a bit has changed
under the skin in 5.1 (especially subentries), and all the *.i and *.is
files have been changed to account for these changes. I would therefore
be very suprised if old indexes were still compatible.

Tim

--
Dr. Tim Dudgeon				Phone: 01865 748747
British Biotech Pharmaceuticals Plc.	FAX:   01865 717598
Watlington Road, Oxford, OX4 5LY, UK	email: dudgeon@britbio.co.uk

-------------------==== Posted via Deja News ====-----------------------
      http://www.dejanews.com/     Search, Read, Post to Usenet

From owner-srs@net.bio.net Wed Jan 14 22:00:00 1998
Path: biosci!bloom-beacon.mit.edu!eru.mt.luth.se!feed1.news.luth.se!luth.se!news-ge.switch.ch!news-zh.switch.ch!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!eerie.fr!cnusc.fr!ciril.fr!u-strasbg.fr!news
From: pingouin@chouchen.u-strasbg.fr (Francois Jeanmougin)
Newsgroups: bionet.software.srs
Subject: Re: blast on srs5.1
Date: 15 Jan 1998 07:39:09 GMT
Organization: CRC - Universite Louis Pasteur - Strasbourg France
Lines: 16
Message-ID: <69keet$nrt@news.u-strasbg.fr>
References: <884798805.425590801@dejanews.com>
Reply-To: jeanmougin@igbmc.u-strasbg.fr
NNTP-Posting-Host: chouchen.u-strasbg.fr
Mime-Version: 1.0
Content-Type: text/plain; charset=iso-8859-1
Content-Transfer-Encoding: 8bit
X-Newsreader: knews 0.9.8

In article <884798805.425590801@dejanews.com>,
	Tim Dudgeon <dudgeon@britbio.co.uk> writes:
> I've come across the solution to a previously posted message about blast
> output not being correctly parsed.

	I think it's not only an expected GCG ouput, it is also because
different version of blast gives different output. We experienced such
problems with DBWatcher...
	If I remember correctly, Fred found a way to parse easily
both outputs, but, Fred is out this week, so he will confirm next
week if I'm right.
						François.

-- 
François Jeanmougin     | groupe de bioinformatique / bioinformatics groupe
tel:(+33) 3 88 65 32 71 | IGBMC BP 163 67404 Illkirch France

From owner-srs@net.bio.net Wed Jan 14 22:00:00 1998
Path: biosci!AMC.UVA.NL!a.h.vankampen
From: a.h.vankampen@AMC.UVA.NL ("A.H. van kampen ", automat)
Newsgroups: bionet.software.srs
Subject: A few questions
Date: 15 Jan 1998 08:19:10 -0800
Organization: Academic Medical Centre Amsterdam
Lines: 38
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <34BE36C4.A79D13E6@amc.uva.nl>
NNTP-Posting-Host: net.bio.net

Hello,

I have a few (basic) questions about setting up a local SRS site.

1) What are the main advantages for having a local copy of
     databases like embl, swissprot, etc. It seems that the response
time
      for searching in a remote database is still acceptable.
2) What is the best strategy for maintaining a local set of databases?
     e.g., is everyone updating their databases with ftp each day.
3) Is everyone using identical configuration files (i.e. *.i and *.is)
files
     for the databases.

Since I'm new to the field, i'm trying to get some idea of maintaining
local databases is worth the effort.

Regards,


Antoine van Kampen

--
   ---------------------------------------
   | Dr. Antoine H.C. van Kampen         |
   | Academic Medical Center Amsterdam   |
   | Cluster Research Support (ADIV-D01) |
   | Meibergdreef 9                      |
   | 1105 AZ  Amsterdam, the Netherlands |
   -------------------------------------------------|
                | Phone:  ++31-20-5667096           |
                | Fax:    ++31-20-5669020           |
                | Email:  a.h.vankampen@amc.uva.nl  |
                | www:    http://www.amc.uva.nl/    |
                -------------------------------------




From owner-srs@net.bio.net Thu Jan 15 22:00:00 1998
Newsgroups: bionet.software.srs
Path: biosci!daresbury!uninett.no!news.maxwell.syr.edu!newsfeed.internetmci.com!192.87.106.104!surfnet.nl!barba.uci.kun.nl!sci.kun.nl!caos.kun.nl!root
From: "K. Cuelenaere" <koenc@caos.kun.nl>
Subject: Re: Indexing with SRS5.1.0
Content-Type: text/plain; charset=us-ascii
Message-ID: <34BF6543.2781@caos.kun.nl>
Sender: root@caos.kun.nl (System PRIVILEGED Account)
NNTP-Posting-Host: cammsg11.caos.kun.nl
Content-Transfer-Encoding: 7bit
Cc: koenc@caos.kun.nl
Organization: CAOS/CAMM Center
References: <1998Jan15.155704@wehi>
Mime-Version: 1.0
Date: Fri, 16 Jan 1998 13:48:51 GMT
X-Mailer: Mozilla 3.0Gold (X11; I; IRIX 6.2 IP22)
Lines: 29

Janice Coventry, Walter and Eliza Hall Institute wrote:
> 
> Dear SRS Managers
> 
> To upgrade to SRS5.1.0 is it necessary to reindex all your files?
> I have been testing some of my databases, when I try
> to getz entries from the old indices I have a core dump.
> 
> Thanking you in anticipation

Retrieving non-sequence entries (like prosite and pdb) seems
to work with us (haven't tested them all though). It looks to
me all the sequence databases have to be re-indexed because
sequences are now treated as objects. So when you try to
use the old indices with the new .i and .is files, you'll
get an error saying that the sequence is not of the "object"
type. Using the old .i and .is files with 5.1 still seems
possible...

Regards,
Koen.
--------------------------------------------------------------
Koen Cuelenaere			Email: koenc@caos.kun.nl
CAOS/CAMM Center		Phone: +31 24 3653383
University of Nijmegen		Fax:   +31 24 3652977
Toernooiveld
6525 ED Nijmegen
The Netherlands			URL: http://www.caos.kun.nl/
--------------------------------------------------------------

From owner-srs@net.bio.net Thu Jan 15 22:00:00 1998
Path: biosci!daresbury!uninett.no!Cabal.CESspool!bofh.vszbr.cz!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!not-for-mail
From: Peter Rice <pmr@sanger.ac.uk>
Newsgroups: bionet.software.srs
Subject: Re: A few questions
Date: 16 Jan 1998 17:03:47 +0000
Organization: The Sanger Centre
Lines: 41
Sender: pmr@unst.sanger.ac.uk
Message-ID: <sub1zy8nyfg.fsf@unst.sanger.ac.uk>
References: <34BE36C4.A79D13E6@amc.uva.nl>
NNTP-Posting-Host: unst.sanger.ac.uk
X-Newsreader: Gnus v5.5/Emacs 20.2

a.h.vankampen@AMC.UVA.NL ("A.H. van kampen ", automat) writes:
> I have a few (basic) questions about setting up a local SRS site.
> 
> 1) What are the main advantages for having a local copy of
>      databases like embl, swissprot, etc. It seems that the response
> time
>       for searching in a remote database is still acceptable.

That applies to searching for a few entries through the Web interface.
But you can do a lot through the command line interface, especially
with links and subsets of the databases very quickly and efficiently,
and this is not available through the Web interface (and therefore
must be done locally).

> 2) What is the best strategy for maintaining a local set of databases?
>      e.g., is everyone updating their databases with ftp each day.

Sites have their own strategies. "best" depends on your own needs.
There is a databank of databanks that shows you when other
sites have updated, so you can follow their strategies.

It often depends on how much you need the very latest entries, and how
long indexing takes.


> 3) Is everyone using identical configuration files (i.e. *.i and *.is)
> files
>      for the databases.

From SRS 5.1 I hope to use the standard .i and .is files (and .it for the
database information). Where I diverge I will pass on the changes through
Thure (who is only a few metres away :-)

SRS 5.1 makes this much easier to do than with, for example, SRS 4.0.8.

-- 
----------------------------------------------------------------------
Peter Rice                | Informatics Division, The Sanger Centre,
E-mail: pmr@sanger.ac.uk  | Wellcome Trust Genome Campus,
Tel: (44) 1223 494967     | Hinxton, Cambridge, CB10 1SA, England
Fax: (44) 1223 494919     | URL: http://www.sanger.ac.uk/Users/pmr/

From owner-srs@net.bio.net Sun Jan 18 22:00:00 1998
Path: biosci!internet!biosci!not-for-mail
From: biohelp (BIOSCI Administrator)
Newsgroups: bionet.software.srs
Subject: BIOSCI/bionet miniFAQ & Fundraiser
Date: 19 Jan 1998 02:00:08 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 233
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199801191000.CAA15831@net.bio.net>
NNTP-Posting-Host: net.bio.net

(LAST REVISION: 30-JUL-95)

This BIOSCI "miniFAQ" is designed to answer the questions that come up
the *most frequently*.  The main BIOSCI FAQ (Frequently Asked
Questions) is accessible on the World Wide Web at URL
http://www.bio.net/.

If you can not find an answer to your question in this or other
documentation, the BIOSCI technical support staff answers e-mail
queries sent to

		       biosci-help@net.bio.net

We can only answer questions about the use of the newsgroups and
mailing lists.  We unfortunately do not have the staff to do Internet
information searches or answer scientific questions.  Please post
those to the appropriate BIOSCI/bionet newsgroups.


	Contents:
	--------
	0) BIOSCI NEEDS YOUR SUPPORT!!

	1) Using the WWW to access the BIOSCI/bionet newsgroups.

	2) What to do about "spams," i.e., junk mail, ads, etc.

	3) Examples of subscribing and unsubscribing to the mailing lists.

	4) The BIOSCI user address and research interest directory.


0) BIOSCI NEEDS YOUR SUPPORT!!
------------------------------
BIOSCI's government funding has been expended, and we are now
operating solely from advertising revenue that we have raised from our
Web site at http://www.bio.net/.  We need just a few minutes of your
time to help us serve you.

You can do two important things which will take very little time for
you individually and will immensely help us continue to help you.

First, please use our WWW system at http://www.bio.net/ to access the
archives.  You can post or reply to messages via your Web browser as
described in item #1 below.  Your usage helps attract sponsors. If you
contact any of our sponsors, please be sure to thank them for
supporting BIOSCI. It is critical for them to get this feedback if
they are to continue their sponsorship for the long term.

Second, if you work for a company or organization that provides
products or services of interest to the biology community, please pass
this message on to your marketing or marketing communications
department or other appropriate group.  Please ask them to help
support BIOSCI by sponsoring our Web site and explain the uses and
benefits of the system to the biology community. If they are
interested, they can then contact us for further information at our
tech support address, biosci-help@net.bio.net.


1) Using the WWW to access the BIOSCI/bionet newsgroups.
--------------------------------------------------------
As of 10 December 1995, all BIOSCI/bionet full newsgroups are
accessible through the World Wide Web (WWW) at URL http://www.bio.net.
One can read and reply publicly or privately to both recent postings
and archived messages through one's Web browser if it is configured
properly to send e-mail.  Each newsgroup is equipped with its own WAIS
index.  The main BIOSCI home page also has access to the BIO-JOURNALS
Table of Contents database WAIS index and the BIOSCI user address
database described in another item further below.


2) What to do about "spams," i.e., junk mail, ads, etc.
-------------------------------------------------------
BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups),
mailing lists, and a hypermail archive at URL http://www.bio.net/.
The same postings are distributed on all media (except for a small
number of mailing-list-only groups at net.bio.net).  Unfortunately it
is becoming a despicable practice on the Internet (by a few people out
to make a fast buck) to do automated mass postings to thousands of
newsgroups and mailing lists.  These attempts to grab free advertising
are refered to as "spams" in the usual, somewhat boneheaded, net
terminology.  USENET is more susceptible to this practice, and many
spams originate on the USENET groups and then are passed on to the
mailing lists.  However, spammers also get lists of mailing addresses
and hit these too, so neither medium is immune.

What should you do personally if you get junk mail?
---------------------------------------------------
Just delete it and move on without reading it further.  Filing a
protest is becoming increasingly useless because spammers are often
disguising the addresses where the messages are sent from.  Unless you
really understand Internet mail systems, your attempt at protest by
sending replies to the message will often end up being sent to the
address of an innocent person that the spammer is victimizing.

What can BIOSCI/bionet do to protect its newsgroups?
----------------------------------------------------
The only solution currently available is to moderate the newsgroup.
If this newsgroup is already moderated, then you are in good shape.
Moderation protects the USENET distribution from about 95% of the
spams that are being sent to date and protects the mailing lists
completely.  Moderation means, however, that someone has to take the
time to review each message before it goes out.  We have set up
software here that simply allows the moderator to forward to an
address at net.bio.net messages that (s)he wishes to have distributed.
This takes no more time than that needed to read the message and pass
it on, say about 1 min. per message.

Most newsgroups currently have a discussion leader who is responsible
for their newsgroup.  The discussions leaders and their e-mail
addresses are listed in the BIOSCI Information Sheet which is
available on the Web at http://www.bio.net/.  If a newsgroup is being
hit with too many junk postings, please contact the discussion leader
for that group and see if there is interest in moderating the group.
Please do not assume that by simply posting a complaint to the
newsgroup itself, anyone on the BIOSCI staff will act on your
complaint.  With close to 100 newsgroups to run, the BIOSCI staff has
to rely on the discussion leaders of each newsgroup to report problems
directly to us at biosci-help@net.bio.net.

We will moderate any of our newsgroups if the discussion leader tells
us that the readership of the group wishes to do so and if a moderator
is willing to do the work.  For most BIOSCI/bionet groups, this
entails only a few minutes of work each day.

Moderating a newsgroup will resolve probably 95% of the junk postings
on the USENET distribution.  Unfortunately there are easy ways for
determined spammers to override the moderation mechanism on USENET,
but we can protect our e-mail subscribers from unwanted postings if
the newsgroup is moderated.  You can also access our newsgroups over
the WWW at URL http://www.bio.net.  While this Web interface will not
stop spammers from trying to post to the groups, this will give you
yet another way, besides using USENET news, to keep the junk out of
your personal mail files.  For those of you with local USENET news
systems, the Web interface will also give you faster access to new
newsgroups and recent postings.


3) Examples of subscribing and unsubscribing to the mailing lists.
------------------------------------------------------------------
PLEASE NOTE: The BIOSCI management does NOT act on
subscription/unsubscription requests that are posted improperly to the
newsgroups and mailing lists.  People who do this only bother everyone
on the lists to no avail.  Please be sure to follow the proper
procedures below.

Gory details are in the BIOSCI Information sheets on the Web at
http://www.bio.net.  Below we give an example utilizing the
METHODS-AND-REAGENTS list at both of our two BIOSCI sites:

Users in the Americas and Pacific Rim countries who use the BIOSCI
------------------------------------------------------------------
node at computer net.bio.net:
----------------------------

A) Determine the "listname" which is the <=8 character mail address
                                         ^^^^^^^^^^^^^
   for the group.  These can be found in the BIOSCI Info. Sheet.  For
   the METHODS-AND-REAGENTS group the mailing address is
   methods@net.bio.net.  The listname is the portion of the address to
   the left of the @ sign, i.e., "methods".  The listname is used with
   the "subscribe" and "unsubscribe" commands illustrated below.

B) Mail all commands in the body of a mail message addressed to
   biosci-server@net.bio.net.  Do NOT send commands to the newsgroup
   posting addresses!  Leave the Subject: line blank, any text on it
   will be ignored.

C) In the body of your message put one or more of the following
   commands with an "end" command on the last line, e.g.,

   subscribe methods
   unsubscribe methods
   end

   Do NOT put your e-mail address or other text on these lines.  The
   server only allows you to cancel your subscription if the address
   on your mail header matches the address on our mailing list.
   Please ask for help at biosci-help@net.bio.net if your address has
   changed, e.g., if you know you are on the list but the server tells
   you that you are not a member.


Users in Europe, Africa, and Central Asia who use the BIOSCI node at
--------------------------------------------------------------------
computer daresbury.ac.uk (also known as dl.ac.uk):
-------------------------------------------------

To subscribe and unsubscribe to/from the BIOSCI lists, you need to
specify the full USENET newsgroup name with "bionet-news." prepended.
The USENET newsgroup names are listed in the BIOSCI Information sheet
on the Web at http://www.bio.net/.  For the METHODS-AND-REAGENTS list
the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the
appropriate commands are

    sub bionet-news.bionet.molbio.methds-reagnts

    unsub bionet-news.bionet.molbio.methds-reagnts

These commands are included in a message addressed to mxt@dl.ac.uk,
NOT to the newsgroup mailing addresses.  As usual, include the text in
the body of the message as text on the Subject: line is ignored.

To unsubscribe from all the lists at the UK node, use

    unsub bionet-news

Please note that if the address in the list is different than the one
in your mail message header, you will not be able to unsubscribe by
this method. If you have problems, please mail biosci@daresbury.ac.uk.


4) The BIOSCI user address and research interest directory.
-----------------------------------------------------------
Please take this opportunity to add your name, address, and research
interest information to the BIOSCI User Address Database if you have
not already done so.

You can fill out the address form directly through our Web page at URL
http://www.bio.net/adrform.html.

The address database is reindexed nightly for WWW access (the URL is
http://www.bio.net/).  If you are not directly on the Internet but can
reach it by e-mail, please use our waismail server to access the user
directory.  waismail use is described above.  You can also request a
user address form by e-mail from biosci-help@net.bio.net.

Please check your database entry from time-to-time to see if your
address information is still up-to-date.  Because of our limited
personnel resources, we ask that you resubmit a *complete* form to
revise your entry; we only replace complete entries and do not have
resources to edit old forms.


From owner-srs@net.bio.net Mon Jan 19 22:00:00 1998
Path: biosci!agate!logbridge.uoregon.edu!Cabal.CESspool!bofh.vszbr.cz!newsfeed.eerie.fr!jussieu.fr!univ-lyon1.fr!cnusc.fr!ciril.fr!u-strasbg.fr!news
From: Frederic PLEWNIAK <plewniak@igbmc.u-strasbg.fr>
Newsgroups: bionet.software.srs
Subject: Re: blast on srs5.1
Date: Tue, 20 Jan 1998 11:15:25 +0100
Organization: IGBMC
Lines: 15
Message-ID: <34C4793D.919@igbmc.u-strasbg.fr>
References: <884798805.425590801@dejanews.com> <69keet$nrt@news.u-strasbg.fr>
NNTP-Posting-Host: didon.u-strasbg.fr
Mime-Version: 1.0
Content-Type: text/plain; charset=iso-8859-1
Content-Transfer-Encoding: 8bit
X-Mailer: Mozilla 3.0 (X11; I; SunOS 5.5 sun4m)

>         I think it's not only an expected GCG ouput, it is also because
> different version of blast gives different output. We experienced such
> problems with DBWatcher...
>         If I remember correctly, Fred found a way to parse easily
> both outputs, but, Fred is out this week, so he will confirm next
> week if I'm right.
>                                                 François.
>
Oh! my... Just comin' back from a sweet quiet week off lab and now...
François, you're too fast to jump onto your keyboard ;o)... Sorry to
let you down but DBWatcher doesn't really parse title lines in BLAST
output. Not the way SRS does anyway! And it is certainly not able
to tell the accession number from the ID... Sorry...

Fred

From owner-srs@net.bio.net Mon Jan 19 22:00:00 1998
Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed4.bbnplanet.com!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.sprintlink.net!news.sprintlink.net!Sprint!newsfeed.nacamar.de!newsfeed.eerie.fr!jussieu.fr!infobiogen.fr!newsmaster
From: Jean-Marc Plaza <plaza@infobiogen.fr>
Newsgroups: bionet.software.srs
Subject: links in srs5.0.1
Date: Tue, 20 Jan 1998 16:35:32 +0100
Organization: INFOBIOGEN
Lines: 70
Message-ID: <34C4C443.4CB5E5B@infobiogen.fr>
NNTP-Posting-Host: lovelace.infobiogen.fr
Mime-Version: 1.0
Content-Type: multipart/alternative; boundary="------------FAD1AFB6B4D91FEF8AF019A0"
X-Mailer: Mozilla 4.04 [en] (X11; I; SunOS 5.5.1 sun4u)


--------------FAD1AFB6B4D91FEF8AF019A0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit

Dear all,


we succeed in installing the new versions of srs5.01 but
we find one problem when you want to valid the selection of databank in
the link window or in the query manager(link): there is an internal
server error message.
(the error_log gives this message:
[Tue Jan 20 15:30:56 1998] access to
/env/infobiogen/pack/srs5/www/bin/wgetz
failed for lovelace.infobiogen.fr, reason: Premature end of script
headers

The same query link  with getz work well.

Thanks in advance for your help,

Jean-Marc

--
---------------------------------------------------
Jean-Marc PLAZA
INFOBIOGEN - CNRS
7, rue Guy Moquet BP8 94801 VILLEJUIF Cedex, France
tel: +33 45 59 52 39  fax: +33 45 59 52 50
e-mail: plaza@infobiogen.fr
---------------------------------------------------



--------------FAD1AFB6B4D91FEF8AF019A0
Content-Type: text/html; charset=us-ascii
Content-Transfer-Encoding: 7bit

<HTML>
Dear all,
<BR>&nbsp;

<P>we succeed in installing the new versions of srs5.01 but
<BR>we find one problem when you want to valid the selection of databank
in
<BR>the link window or in the query manager(link): there is an internal
server error message.
<BR>(the error_log gives this message:
<BR>[Tue Jan 20 15:30:56 1998] access to /env/infobiogen/pack/srs5/www/bin/wgetz
<BR>failed for lovelace.infobiogen.fr, reason: Premature end of script
headers

<P>The same query link&nbsp; with getz work well.

<P>Thanks in advance for your help,

<P>Jean-Marc
<PRE>--&nbsp;
---------------------------------------------------
Jean-Marc PLAZA
INFOBIOGEN - CNRS
7, rue Guy Moquet BP8 94801 VILLEJUIF Cedex, France
tel: +33 45 59 52 39&nbsp; fax: +33 45 59 52 50
e-mail: plaza@infobiogen.fr
---------------------------------------------------</PRE>
&nbsp;</HTML>

--------------FAD1AFB6B4D91FEF8AF019A0--


From owner-srs@net.bio.net Mon Jan 19 22:00:00 1998
Path: biosci!agate!howland.erols.net!nntp.abs.net!newspeer.monmouth.com!Cabal.CESspool!bofh.vszbr.cz!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!not-for-mail
From: "Thure Etzold" <etzold@ebi.ac.uk>
Newsgroups: bionet.software.srs
Subject: Re: blast on srs5.1
Date: Wed, 21 Jan 1998 00:12:28 -0000
Organization: MRC Human Genome Mapping Project Resource Centre
Lines: 31
Message-ID: <6a24g9$9rp$1@niobium.hgmp.mrc.ac.uk>
References: <884798805.425590801@dejanews.com> <69keet$nrt@news.u-strasbg.fr> <34C4793D.919@igbmc.u-strasbg.fr>
NNTP-Posting-Host: idefix.ebi.ac.uk
X-Newsreader: Microsoft Outlook Express 4.71.1712.3
X-MimeOLE: Produced By Microsoft MimeOLE V4.71.1712.3


Frederic PLEWNIAK wrote in message <34C4793D.919@igbmc.u-strasbg.fr>...
>>         I think it's not only an expected GCG ouput, it is also because
>> different version of blast gives different output. We experienced such
>> problems with DBWatcher...
>>         If I remember correctly, Fred found a way to parse easily
>> both outputs, but, Fred is out this week, so he will confirm next
>> week if I'm right.
>>                                                 François.
>>
>Oh! my... Just comin' back from a sweet quiet week off lab and now...
>François, you're too fast to jump onto your keyboard ;o)... Sorry to
>let you down but DBWatcher doesn't really parse title lines in BLAST
>output. Not the way SRS does anyway! And it is certainly not able
>to tell the accession number from the ID... Sorry...


well, too bad! I think the only way to get around this is to create separate
versions of .i and .is for different versions of blast.
This would be reasonable since not only the format of the output but also
the program options change

parsing the title line for entry names and accession numbers is another
problem and could be alleviated by including productions for both in the
syntax where only one is active at a time. I used a similar solution for
switching between gcg and native format for most sequence database .

regards
thure



From owner-srs@net.bio.net Tue Jan 20 22:00:00 1998
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!4.1.16.34!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!uunet!in2.uu.net!nntp.earthlink.net!usenet
From: sales@inforia.com
Newsgroups: bionet.software.srs
Subject: ZDNet Hotfile & Editor's Pick!!
Date: Wed, 21 Jan 1998 19:02:33 GMT
Organization: EarthLink Network, Inc.
Lines: 29
Message-ID: <34c64644.7090536@news.earthlink.net>
NNTP-Posting-Host: 207.217.234.33
X-Newsreader: Forte Agent .99e/32.227

********************************************************
Inforia Quest won ZDNet Hotfile Editor's Pick!!
********************************************************

How Preston Gralla talks about Inforia Quest: 

    It lets you look through dozens of search
    engines and sites simultaneously, and
    customize your search to use only those sites
    and engines you want. You can confine your
    search to news sites, entertainment sites,
    Usenet newsgroups, or technology sites. And
    you can create customized searches of your
    own. The program reports not just the links it
    finds, but also the relevance to your search, the
    date the page was updated, and the page size.
    Most useful is the summary it provides for each
    page. And it even checks the links it finds to
    see whether they're still live. I use this program 
    several times every day. It's a keeper.

(From: http://www.hotfiles.com/prespick/pres0198.html)

So why hesitate? Download IQ now for a free trial and
enjoy the 5-star award program.

For further product info, go to http://www.inforia.com/quest.

Wish you had a happy Internet surfing!

From owner-srs@net.bio.net Wed Jan 21 22:00:00 1998
Path: biosci!agate!howland.erols.net!newsfeed.nacamar.de!news-kar1.dfn.de!news.embl-heidelberg.de!usenet
From: Weare@EuropeMail.com (Johnathon Weare)
Newsgroups: bionet.software.srs
Subject: javascript required message
Date: 22 Jan 1998 16:46:55 +0100
Organization: Biocomputing, EMBL Heidelberg, Germany
Lines: 13
Sender: weare@fifi.embl-heidelberg.de
Distribution: world
Message-ID: <w17zpkoo6j4.fsf@fifi.embl-heidelberg.de>
NNTP-Posting-Host: fifi.embl-heidelberg.de
To: etzold@ebi.ac.uk
Cc: chenna
Encryption-by: McWurst
McWurst-Schluessel: O*Uef!$p&^afC1fe@0n--9`#gA||~[gf)*fcq26
X-Newsreader: Gnus v5.3/Emacs 19.34



By my calculations at least 10% of users cannot use SRS 5.10 at all - they
just get a blank page. 

When users have old browsers or javascript is turned off, they click on
start and get a blank page. I suggest a short message for these non
javascript browsers.

Johnny

-- 
Johnathon Weare -- EMBL -- Weare@EuropeMail.com --- http://www.gpcr.org/johnny/

From owner-srs@net.bio.net Wed Jan 21 22:00:00 1998
Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed4.bbnplanet.com!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!Cabal.CESspool!bofh.vszbr.cz!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!not-for-mail
From: "Thure Etzold" <etzold@ebi.ac.uk>
Newsgroups: bionet.software.srs
Subject: Re: javascript required message
Date: Fri, 23 Jan 1998 07:17:15 -0000
Organization: MRC Human Genome Mapping Project Resource Centre
Lines: 23
Message-ID: <6a864s$cu7$1@niobium.hgmp.mrc.ac.uk>
References: <w17zpkoo6j4.fsf@fifi.embl-heidelberg.de>
NNTP-Posting-Host: idefix.ebi.ac.uk
X-Newsreader: Microsoft Outlook Express 4.71.1712.3
X-MimeOLE: Produced By Microsoft MimeOLE V4.71.1712.3


Johnathon Weare wrote in message ...
>
>
>By my calculations at least 10% of users cannot use SRS 5.10 at all - they
>just get a blank page.
>
>When users have old browsers or javascript is turned off, they click on
>start and get a blank page. I suggest a short message for these non
>javascript browsers.


point taken!
problem should be only if javascript is turned off. there are two versions
of the top page with and without javascript. The one with javascript is
chosen only if the client has something like "Mozilla4" in the
HTTP_USER_AGENT ...this selects all netscape4, communicator and most ie4
browsers

regards
Thure



From owner-srs@net.bio.net Thu Jan 22 22:00:00 1998
Path: biosci!agate!howland.erols.net!news-peer.sprintlink.net!news.sprintlink.net!Sprint!newsfeed.nacamar.de!news-kar1.dfn.de!news.embl-heidelberg.de!usenet
From: Weare@EuropeMail.com (Johnathon Weare)
Newsgroups: bionet.software.srs
Subject: Re: javascript required message
Date: 23 Jan 1998 14:20:08 +0100
Organization: Biocomputing, EMBL Heidelberg, Germany
Lines: 27
Sender: weare@fifi.embl-heidelberg.de
Distribution: world
Message-ID: <w17u3avnx87.fsf@fifi.embl-heidelberg.de>
References: <w17zpkoo6j4.fsf@fifi.embl-heidelberg.de>
	<6a864s$cu7$1@niobium.hgmp.mrc.ac.uk>
NNTP-Posting-Host: fifi.embl-heidelberg.de
To: etzold@ebi.ac.uk
Encryption-by: McWurst
McWurst-Schluessel: O*Uef!$p&^afC1fe@0n--9`#gA||~[gf)*fcq26
X-Newsreader: Gnus v5.3/Emacs 19.34





>>>>> "TE" == Thure Etzold <etzold@ebi.ac.uk> writes:

    TE> Johnathon Weare wrote in message ...
    >> By my calculations at least 10% of users cannot use SRS 5.10 at all
    >> - they just get a blank page.

    TE> point taken!  problem should be only if javascript is turned
    TE> off. there are two versions of the top page with and without
    TE> javascript. The one with javascript is chosen only if the client
    TE> has something like "Mozilla4" in the HTTP_USER_AGENT ...this
    TE> selects all netscape4, communicator and most ie4 browsers

OK - so take 10% off my estimate and add one person!

Any chance of a non javascript link to the alternate start page? Could be
hidden by a javascript part for elegance.

Must say I like the interface changes Thure, 

cheers, Johnny

-- 
Johnathon Weare -- EMBL -- Weare@EuropeMail.com --- http://www.gpcr.org/johnny/

From owner-srs@net.bio.net Mon Jan 26 22:00:00 1998
Path: biosci!daresbury!uninett.no!news.maxwell.syr.edu!news-peer.sprintlink.net!news-backup-east.sprintlink.net!news-in-east.sprintlink.net!news.sprintlink.net!Sprint!129.174.1.8!portal.gmu.edu!news
From: Joseph Nepomuceno <xbonex@hotmail.com>
Newsgroups: bionet.software.srs
Subject: -----Free Software Download------
Date: 27 Jan 1998 04:09:26 GMT
Organization: George Mason University, Fairfax, Virginia, USA
Lines: 31
Message-ID: <6ajmlm$5fa@portal.gmu.edu>
NNTP-Posting-Host: osf1.gmu.edu
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 1.22 (Windows; I; 16bit)

> ____________________________________________________________
> ******* Announcing the Mortgage Manager(TM) Software *******
> ------------------------------------------------------------
> Would you like to try our mortgage analysis software?
> It's FREE and all you have to do is download it from our site
> at <http://www.angelfire.com/biz/freesoftware95/download.html>
> 
> 
> The software will analyze your home mortgage and provide
> you with a FREE service that will allow you to...
> 
> 
> * Save thousands of dollars without refinancing.
> * Cut years off your mortgage without increasing your payment.
> * Build equity in your home 300% faster
> * Audit your loan automatically to find lender mistakes
>   which occur about 50% of the time according to the F.D.I.C.
> 
> 
> 
> For more information please go to:
> http://www.angelfire.com/biz/freesoftware95
> 
> 
> To download now please go to:
> http://www.angelfire.com/biz/freesoftware95/download.html
> 
> *When running the software remember to use CODE: 22701...
>  this will unlock the software.



From owner-srs@net.bio.net Tue Jan 27 22:00:00 1998
Path: biosci!agate!logbridge.uoregon.edu!europa.clark.net!208.134.241.18!newsfeed.internetmci.com!199.117.161.1!csn!nntp-xfer-1.csn.net!news.acsu.buffalo.edu!srv1.drenet.dnd.ca!crc-news.doc.ca!nott!utnut!bioinfo.med.utoronto.ca!resunix.sickkids.on.ca!manuel_buchwald
From: ali@genet.sickkids.on.ca (Alex Dong Li)
Newsgroups: bionet.software.srs
Subject: Webblast--a program to do gapped blast from unix command line
Date: Thu, 29 Jan 1998 01:18:39 GMT
Organization: The Hospital For Sick Children
Lines: 33
Message-ID: <6aoann$2a5$13@resunix.sickkids.on.ca>
NNTP-Posting-Host: 142.20.38.249
X-Newsreader: News Xpress 2.01

Webblast--a program to do gapped blast from unix command line

webblast, written in perl, does NCBI gapped blast search (version 2)
by mimicing a www client (such as Netscape &IE) from the unix
command line, and saves the results as local files.
Most options are similar to those available from NCBI blast page.
Currently the graphical overview is not available.

Some examples are:
webblast -format=fasta sample1.seq
webblast -prog=blastn -datab=nr -align=25 -notask
webblast -prog=blastp -datab=swissprot -email=smith@dept.univ.edu

Input sequence file(s) can be in GCG, fasta or plain text format .
(you need to specify by "-format=fasta" or "-format=text" if files
are in last two formats). If no filename is specified,
all files in current directory will be blasted.

It is available at
http://www.genet.sickkids.on.ca/bioinfo_resources/software.html

To install the program on your own machine,
   0) install perl for unix (For most networked unix machines, perl
         should have been installed. If not, contact your system admin),
   1) copy webblast and postclient.pl to your system,
   2) change the file permissions so that you can run them,
   3) modify the two files according to the instruction in the headers

If you have any suggestions/comments, please let me know.

Regards,

Alex Dong Li                                                   

