From owner-bio-srs@hgmp.mrc.ac.uk Thu Oct 24 12:06:03 2002 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 409847D0E3 for ; Thu, 24 Oct 2002 12:06:03 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 941237D0E5 for ; Thu, 24 Oct 2002 12:06:02 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6015) id B2CD67D0DC; Thu, 24 Oct 2002 12:06:00 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 6E59517AA1 for ; Wed, 16 Oct 2002 16:41:27 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 0760517A9C; Wed, 16 Oct 2002 16:41:18 +0100 (BST) To: bionet-software-srs@net.bio.net From: gene.quantification@wzw.tum.de (Gene Quantification Homepage) Newsgroups: bionet.software.srs Subject: Gene Quantification Newsletter - 10/2002 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <5.0.2.1.0.20021016174144.03e4a4a0@pollux.wzw.tum.de> Mime-Version: 1.0 Content-Type: multipart/alternative; boundary="=====================_30211703==_.ALT" X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 6E72E17AAC; Wed, 16 Oct 2002 16:41:13 +0100 (BST) X-Received: from pollux.wzw.tum.de (pollux.edv.agrar.tu-muenchen.de [141.40.103.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 7CAC317A9C; Wed, 16 Oct 2002 16:40:55 +0100 (BST) X-Received: from pfaffl.wzw.tum.de ([141.40.217.71]) by pollux.wzw.tum.de (AIX4.3/8.9.3/8.9.3) with ESMTP id QAA11956; Wed, 16 Oct 2002 16:40:54 +0100 X-Sender: genequan@pollux.wzw.tum.de X-Mailer: QUALCOMM Windows Eudora Version 5.0.2 X-To: bio-srs@net.bio.net, bionauts@net.bio.net, cellbiol@net.bio.net, immuno@net.bio.net Date: Thu, 24 Oct 2002 12:06:00 +0100 (BST) X-Razor-id: 3c1aa0a938708a6748bb0ba22ffb01fcf43ced65 Sender: owner-bio-srs@hgmp.mrc.ac.uk Precedence: bulk --=====================_30211703==_.ALT Content-Type: text/plain; charset="us-ascii"; format=flowed Gene Quantification Newsletter - 10/2002 October 2002 Introduction: Main focus of the GENE QUANTIFICATION web page http://www.wzw.tum.de/gene-quantification/ is, to describe and summarize all technical aspects involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR: e.g. detection chemistry, assay specificity, sensitivity, reproducibility, robustness, intra- & inter-assay variations, kinetic PCR efficiency calculation, quantification strategies, optimisation strategy, data calculation and statistics, etc. etc. The GENE QUANTIFICATION web page illustrates the usefulness of reliable quantification strategies, e.g. absolute-, relative-quantification assays, and the difference between them in kinetic PCR & kinetic RT-PCR. Main focus of the PHYSIOLOGY web page is, to describe all kind of kinetic RT-PCR applications in molecular Physiology, molecular Endocrinology, and molecular Immunology (mainly mammary gland immunology) are summarized on my new homepage http://www.wzw.tum.de/gene-quantification/physiology.shtml ############################################# NEW features: Determination of real-time PCR efficiency - NEW EQUATIONS http://www.wzw.tum.de/gene-quantification/efficiency.html Endocrinology & Mammary Gland Immunology - NEW PUBLICATIONS http://www.wzw.tum.de/gene-quantification/physiology.shtml Steroid receptors: AR, ERa, ERb, PR Adrenergic receptors: alpha-1-subtypes & alpha-2-subtypes & beta-subtypes (publications in press) Somatotropic axis: GHR, IGF-1, IGF2, both IGF receptors & IGF-BP1 to BP3 Serotonin receptors (coming soon) NEW MOVIES http://www.wzw.tum.de/gene-quantification/movie.html e.g. PCR, RPA, Electrophoresis, Northern- & Southern-Blot NEW PUBLICATIONS about kinetic PCR http://www.wzw.tum.de/gene-quantification/real-time.html and http://www.wzw.tum.de/gene-quantification/chemistry.html and http://www.wzw.tum.de/gene-quantification/hkg.html and http://www.wzw.tum.de/gene-quantification/literature.html sections: RT-PCR and real-time RT-PCR (in general) LightCycler specific technical notes absolute quantification using kinetic PCR relative quantification using kinetic PCR classical block RT-PCR & competitive RT-PCR normalisation & housekeeping gene problems in real-time PCR verification of DNA array results quantification software: REST, REST-XL, Q-Gene, geNorm kinetic RT-PCR poster board Poster boards (a little bit slow !!!): http://www.wzw.tum.de/gene-quantification/poster-a.html methodological poster board A: Array verification physiological poster board B: IGFs - Early Response Genes physiological poster board C: Steroid receptors physiological poster board D: Zinc metabolism physiological poster board E: Immunological relevant factors New links & databases: http://www.wzw.tum.de/gene-quantification/link.html e.g. - Dictionary of Nucleic Acid Structures (DNAS) - Glossary iSenseIT - Molecular Biology Glossary - A Hypermedia Glossary of Genetic Terms pick from an alphabetical list of Terms with 656 entries http://www.realtimeprimerdatabase.ht.st http://www.realtimeprimers.org/ http://www.pcrlinks.com http://www.cyclertest.com/ The COMPLETE Alkami Quick GuideTM for PCR is a (158 pages) http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf LightCycler University (LCU) http://www.wzw.tum.de/gene-quantification/real-time.html#lcu Please send the NEWSLETTER and the home page links to further scientists and friends who are interested in real-time PCR, new quantification strategies, relative or absolute gene quantification using kinetic PCR. best regards Michael Pfaffl ############################################# For further QUESTIONS concerning real-time PCR and if you want to SUBSCRIBE to our monthly appearing GENE QUANTIFICATION NEWSLETTER please contact gene.quantification@wzw.tum.de You will receive all NEW FEATURES and future REST & REST-XL updates. GENE QUANTIFICATION web page and the GENE QUANTIFICATION NEWSLETTER are non-commercial, educational sites with the only purpose of facilitating access to biology-related information over the internet. The web page is optimised for Netscape Navigator version 4.7 & Internet Explorer version 5.0 (and higher versions) with an optimal screen resolution of 1024 x 768 pixels. GENE QUANTIFICATION web page visits and DOWNLOAD statistics are listed here: http://www.wzw.tum.de/gene-quantification/contact.html#server If you want UN-SUBSCRIBE from all future NEWSLETTERS please contact gene.quantification@wzw.tum.de and type REMOVE into the subject line. You will be removed instantly from our mailing list. ******************************************************************** Gene Quantification @ wzw.tum.de e-mail: gene.quantification@wzw.tum.de homepage: http://www.wzw.tum.de/gene-quantification/index.shtml homepage: http://www.wzw.tum.de/gene-quantification/physiology.shtml responsible Senior Scientist: Dr. Michael W. Pfaffl Institute of Physiology Life Science Center Weihenstephan, Technical University of Munich Weihenstephaner Berg 3 D-85350 Freising-Weihenstephan GERMANY ******************************************************************** --=====================_30211703==_.ALT Content-Type: text/html; charset="us-ascii" Gene Quantification Newsletter - 10/2002
October 2002

Introduction:
Main focus of the GENE QUANTIFICATION web page   http://www.wzw.tum.de/gene-quantification/   is, to describe and summarize all technical aspects involved in quantitative gene expression analysis using real-time (kinetic) PCR  &  RT-PCR:  e.g. detection chemistry, assay specificity, sensitivity, reproducibility, robustness, intra- & inter-assay variations, kinetic PCR efficiency calculation, quantification strategies, optimisation strategy, data calculation and statistics,  etc. etc.

The GENE QUANTIFICATION web page illustrates the usefulness of reliable quantification strategies, e.g. absolute-, relative-quantification assays, and the difference between them in kinetic PCR & kinetic RT-PCR.

Main focus of the PHYSIOLOGY web page is, to describe all kind of kinetic RT-PCR applications in molecular Physiology, molecular Endocrinology, and molecular Immunology (mainly mammary gland immunology) are summarized on my new homepage   http://www.wzw.tum.de/gene-quantification/physiology.shtml

#############################################

NEW features:

Determination of real-time PCR efficiency  -  NEW EQUATIONS
http://www.wzw.tum.de/gene-quantification/efficiency.html


Endocrinology & Mammary Gland Immunology  -  NEW PUBLICATIONS
http://www.wzw.tum.de/gene-quantification/physiology.shtml
Steroid receptors:   AR, ERa, ERb, PR
Adrenergic receptors:   alpha-1-subtypes  &  alpha-2-subtypes  &  beta-subtypes   (publications in press)
Somatotropic axis:   GHR, IGF-1, IGF2, both IGF receptors  & IGF-BP1 to BP3
Serotonin receptors   (coming soon)

NEW MOVIES
http://www.wzw.tum.de/gene-quantification/movie.html
e.g.  PCR, RPA, Electrophoresis, Northern- & Southern-Blot


NEW PUBLICATIONS about kinetic PCR
http://www.wzw.tum.de/gene-quantification/real-time.html
and
http://www.wzw.tum.de/gene-quantification/chemistry.html
and
http://www.wzw.tum.de/gene-quantification/hkg.html
and
http://www.wzw.tum.de/gene-quantification/literature.html
sections:
     RT-PCR and real-time RT-PCR (in general)
     LightCycler specific technical notes
     absolute quantification using kinetic PCR
     relative quantification using kinetic PCR
     classical block RT-PCR   &   competitive RT-PCR
     normalisation  &  housekeeping gene problems in real-time PCR
     verification of DNA array results
     quantification software: REST,    REST-XL,   Q-Gene,   geNorm
     kinetic  RT-PCR  poster board


Poster boards (a little bit slow !!!):
http://www.wzw.tum.de/gene-quantification/poster-a.html
methodological  poster board A:  Array verification
physiological  poster board B:     IGFs - Early Response Genes
physiological  poster board C:     Steroid receptors
physiological  poster board D:     Zinc metabolism
physiological  poster board E:     Immunological relevant factors

New links & databases:
http://www.wzw.tum.de/gene-quantification/link.html
e.g.
-  Dictionary of Nucleic Acid Structures (DNAS)
-  Glossary iSenseIT
-  Molecular Biology Glossary
-  A Hypermedia Glossary of Genetic Terms pick from an alphabetical list of Terms with 656 entries
http://www.realtimeprimerdatabase.ht.st
http://www.realtimeprimers.org/
http://www.pcrlinks.com
http://www.cyclertest.com/
The COMPLETE Alkami Quick GuideTM for PCR is a (158 pages)
http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf
LightCycler University (LCU)
http://www.wzw.tum.de/gene-quantification/real-time.html#lcu


Please send the NEWSLETTER and the home page links to further scientists and friends who are interested in real-time PCR, new quantification strategies, relative or absolute gene quantification using kinetic PCR.

best regards

Michael Pfaffl

#############################################

For further QUESTIONS concerning real-time PCR and if you want to SUBSCRIBE to our monthly appearing GENE QUANTIFICATION NEWSLETTER please contact gene.quantification@wzw.tum.de   You will receive all NEW FEATURES and future REST &  REST-XL updates.

GENE QUANTIFICATION web page and the GENE QUANTIFICATION NEWSLETTER are non-commercial, educational sites with the only purpose of facilitating access to biology-related information over the internet. The web page is optimised for Netscape Navigator version 4.7 & Internet Explorer version 5.0 (and higher versions) with an optimal screen resolution of 1024 x 768 pixels.

GENE QUANTIFICATION web page visits and DOWNLOAD statistics are listed here: http://www.wzw.tum.de/gene-quantification/contact.html#server

If you want UN-SUBSCRIBE from all future NEWSLETTERS please contact gene.quantification@wzw.tum.de and type REMOVE into the subject line. You will be removed instantly from our mailing list.


********************************************************************
Gene Quantification @ wzw.tum.de

e-mail: gene.quantification@wzw.tum.de
homepage:       http://www.wzw.tum.de/gene-quantification/index.shtml
homepage:       http://www.wzw.tum.de/gene-quantification/physiology.shtml

responsible Senior Scientist:
Dr. Michael W. Pfaffl
Institute of Physiology
Life Science Center Weihenstephan,
Technical University of Munich
Weihenstephaner Berg 3
D-85350 Freising-Weihenstephan
GERMANY

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