From owner-bio-srs@hgmp.mrc.ac.uk Tue Apr 22 09:49:25 2003 Return-Path: X-Original-To: bio-srs-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id ECE4B7D142 for ; Tue, 22 Apr 2003 09:49:24 +0100 (BST) X-Original-To: bio-srs-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 54B277D12C for ; Tue, 22 Apr 2003 09:49:24 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6015) id C0CF67D0E7; Tue, 22 Apr 2003 09:49:19 +0100 (BST) X-Original-To: bionet-software-srs@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 88CB17D359 for ; Thu, 17 Apr 2003 09:46:45 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 074D67D1D6; Thu, 17 Apr 2003 09:46:42 +0100 (BST) To: bionet-software-srs@net.bio.net From: gene.quantification@wzw.tum.de (Gene Quantification Homepage) Newsgroups: bionet.software.srs Subject: Eastern 2003 - Newsletter Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <5.0.2.1.0.20030417104721.029dd4b0@pollux.wzw.tum.de> Mime-Version: 1.0 Content-Type: multipart/alternative; boundary="=====================_7835890==_.ALT" X-Original-To: bio-srs@net.bio.net X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 827837D359; Thu, 17 Apr 2003 09:46:41 +0100 (BST) X-Received: from pollux.wzw.tum.de (pollux.edv.agrar.tu-muenchen.de [141.40.103.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id B62F07D1D6; Thu, 17 Apr 2003 09:46:35 +0100 (BST) X-Received: from pfaffl.wzw.tum.de ([141.40.217.71]) by pollux.wzw.tum.de (AIX4.3/8.9.3/8.9.3) with ESMTP id KAA66302; Thu, 17 Apr 2003 10:46:34 +0200 X-Sender: genequan@pollux.wzw.tum.de X-Mailer: QUALCOMM Windows Eudora Version 5.0.2 X-To: bio-srs@net.bio.net, bionauts@net.bio.net, cellbiol@net.bio.net, immuno@net.bio.net Date: Tue, 22 Apr 2003 09:49:19 +0100 (BST) Sender: owner-bio-srs@hgmp.mrc.ac.uk Precedence: bulk --=====================_7835890==_.ALT Content-Type: text/plain; charset="iso-8859-1"; format=flowed Content-Transfer-Encoding: quoted-printable Gene Quantification Newsletter Eastern 2003 Happy Eastern to all my visitors and software users !!! We are happy to welcome the 65,000th visitor on my Gene Quantification Pages During the last 14 months !!! Thank you very much !!! New features: A lot of NEW real-time PCR workshops http://www.wzw.tum.de/gene-quantification/main.shtml#work A seminar in the calculation and normalization of real-time RT-PCR results http://folk.uio.no/kristban/realtime/index.html 2nd Nucleic Acid Quantification Meeting in London, UK http://www.mds.qmw.ac.uk/surgery/events/Announcement.html Amplifications for real-time qPCR Analysis workshop https://dynamic.mjr.com/workshop/home.jsp Quantitative real-time PCR course at RoboScreen http://www.roboscreen.com/home/frame.htm etc. etc. etc. New Section BioInformatics in real-time PCR http://www.wzw.tum.de/gene-quantification/bioinformatics.shtml BestKeeper=A9 software - Determination of most stable housekeeping genes,=20 differentially regulated target genes and template=20 integrity: BestKeeper=A9 Excel =A9 spreadsheet tool using a Multiple=20 Correlation and Regression Analysis http://www.wzw.tum.de/gene-quantification/bestkeeper.html Determination of most stable housekeeping genes, differentially regulated=20 target genes and sample integrity: BestKeeper=A9 - Excel=A9 spreadsheet tool= =20 using a Repeated Pair-wise Correlation and Regression Analysis 21. Dechema Jahrestagung der Biotechnologen 2.-4. April 2003 http://www.wzw.tum.de/gene-quantification/poster-f.html LinRegPCR - Assumption-free analysis of quantitative real-time PCR data http://www.wzw.tum.de/gene-quantification/efficiency.html#e-fluoro-4 Determination of real-time PCR efficiency http://www.wzw.tum.de/gene-quantification/efficiency.html NEW EQUATIONS & ALGORITHM by Tichopad et al. 2002 http://www.wzw.tum.de/gene-quantification/efficiency.html#e-fluoro-sig-2 NEW PUBLICATIONS about kinetic PCR http://www.wzw.tum.de/gene-quantification/real-time.html and http://www.wzw.tum.de/gene-quantification/literature.html Gene Quantification in Endocrinology & Immunology Tissue-specific expression pattern of bovine prion gene=20 (PrP): Quantification using real-time RT-PCR http://www.wzw.tum.de/gene-quantification/absolute.html#prp Detection and quantification of mRNA-expression of alpha- and= beta-adrenergic receptor subtypes in the mammary gland of dairy cows. http://www.wzw.tum.de/gene-quantification/endo.html#adrenerg The gastrointestinal tract as target of steroid hormone action:=20 Quantification of steroid receptor mRNA expression (AR, ER-alpha, ER-beta=20 and PR) in 10 bovine gastrointestinal tract compartments by kinetic RT-PCR http://www.wzw.tum.de/gene-quantification/endo.html#erab Characterisation of gene expression patterns in 22RV1 cells for=20 determination of environmental androgenic / antiandrogenic compounds http://www.wzw.tum.de/gene-quantification/endo.html#compounds Gene Expression of Immunologically Important Factors in Blood Cells, Milk=20 Cells and Mammary Tissue of Cows. http://www.wzw.tum.de/gene-quantification/immuno.html#papers Insulin-like growth factor and insulin receptors in intestinal mucosa of=20 neonatal calves. http://www.wzw.tum.de/gene-quantification/endo.html#igfs Please send the NEWSLETTER and the home page links to further scientists=20 and friends who are interested in real-time PCR, new quantification=20 strategies, relative or absolute gene quantification using kinetic PCR. best regards Michael Pfaffl ############################################# For further QUESTIONS concerning real-time PCR and if you want to SUBSCRIBE= =20 to our monthly appearing GENE QUANTIFICATION NEWSLETTER please contact=20 gene.quantification@wzw.tum.de You will receive all NEW FEATURES and=20 future REST & REST-XL & BestKeeper updates. GENE QUANTIFICATION web page and the GENE QUANTIFICATION NEWSLETTER are=20 non-commercial, educational sites with the only purpose of facilitating=20 access to biology-related information over the internet. The web page is=20 optimised for Netscape Navigator version 4.7 & Internet Explorer version=20 5.0 (and higher versions) with an optimal screen resolution of 1024 x 768=20 pixels. GENE QUANTIFICATION web page visits and DOWNLOAD statistics are listed=20 here: http://www.wzw.tum.de/gene-quantification/contact.html#server If you want UN-SUBSCRIBE from all future NEWSLETTERS please contact=20 gene.quantification@wzw.tum.de and type REMOVE into the subject line. You=20 will be removed instantly from our mailing list. ******************************************************************** Gene Quantification @ wzw.tum.de e-mail: gene.quantification@wzw.tum.de homepage: http://www.wzw.tum.de/gene-quantification/index.shtml homepage: http://www.wzw.tum.de/gene-quantification/physiology.shtml homepage: = http://www.wzw.tum.de/gene-quantification/bioinformatics.shtml responsible Senior Scientist: Dr. Michael W. Pfaffl Institute of Physiology Life Science Center Weihenstephan, Technical University of Munich Weihenstephaner Berg 3 D-85350 Freising-Weihenstephan GERMANY ******************************************************************** --=====================_7835890==_.ALT Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Gene Quantification Newsletter  Eastern 2003


Happy Eastern to all my visitors and software users !!!
We are happy to welcome the 65,000th visitor on my Gene Quantification Pages
During the last 14 months !!!

Thank you very much !!!

New features:

A lot of NEW real-time PCR workshops
http://www.wzw.tum.de/gene-quantification/main.shtml#wor= k

A seminar in the calculation and normalization of real-time RT-PCR results
http://folk.uio.no/kristban/realtime/index.html

2nd Nucleic Acid Quantification Meeting  in  London, UK
http://www.mds.qmw.ac.uk/surgery/events/Announcement.html

Amplifications for real-time qPCR Analysis workshop
https://dynamic.mjr.com/workshop/home.jsp
Quantitative real-time PCR course at RoboScreen
http://www.roboscreen.com/home/frame.htm
etc.   etc.   etc.


New Section BioInformatics in real-time PCR

http://www.wzw.tum.de/gene-quantification/bioinformatics= .shtml

BestKeeper=A9 software - Determination of most stable housekeeping genes, differentially regulated target genes and template integrity:  BestKeeper=A9    Excel =A9 spreadsheet tool using a Multiple Correlation and Regression Analysis
http://www.wzw.tum.de/gene-quantification/bestkeeper.html

Determination of most stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper=A9 - Excel=A9 spreadsheet tool using a Repeated Pair-wise Correlation and Regression Analysis
21. Dechema Jahrestagung der Biotechnologen 2.-4. April 2003
http://www.wzw.tum.de/gene-quantification/poster-f.<= a href=3D"http://www.wzw.tum.de/gene-quantification/poster-f.html"= eudora=3D"autourl">html

LinRegPCR  -  Assumption-free analysis of quantitative real-time PCR data
http://www.wzw.tum.de/gene-quantification/efficiency.htm= l#e-fluoro-4

Determination of real-time PCR efficiency
http://www.wzw.tum.de/gene-quantification/efficiency.html

NEW EQUATIONS & ALGORITHM by Tichopad et al. 2002
http://www.wzw.tum.de/gene-quantification/efficiency.htm= l#e-fluoro-sig-2


NEW PUBLICATIONS about kinetic PCR
http://www.wzw.tum.de/gene-quantification/real-time.= html
and
http://www.wzw.tum.de/gene-quantification/literature.html


Gene Quantification in Endocrinology & Immunology

Tissue-specific expression pattern of bovine prion gene (PrP):  Quantification using real-time RT-PCR
http://www.wzw.tum.de/gene-quantification/absolute.html#= prp

Detection and quantification of mRNA-expression of alpha- and beta-adrenergic
receptor subtypes in the mammary gland of dairy cows.
http://www.wzw.tum.de/gene-quantification/endo.html#= adrenerg

The gastrointestinal tract as target of steroid hormone action: Quantification of steroid receptor mRNA expression (AR, ER-alpha, ER-beta and PR) in 10 bovine gastrointestinal tract compartments by kinetic RT-PCR
http://www.wzw.tum.de/gene-quantification/endo.html#= erab

Characterisation of gene expression patterns in 22RV1 cells for determination of environmental androgenic / antiandrogenic compounds
http://www.wzw.tum.de/gene-quantification/endo.html#comp= ounds

Gene Expression of Immunologically Important Factors in Blood Cells, Milk Cells and Mammary Tissue of Cows.
http://www.wzw.tum.de/gene-quantification/immuno.html#pa= pers

Insulin-like growth factor and insulin receptors in intestinal mucosa of neonatal calves.
http://www.wzw.tum.de/gene-quantification/endo.html#= igfs


Please send the NEWSLETTER and the home page links to further scientists and friends who are interested in real-time PCR, new quantification strategies, relative or absolute gene quantification using kinetic PCR.

best regards

Michael Pfaffl

#############################################

For further QUESTIONS concerning real-time PCR and if you want to SUBSCRIBE to our monthly appearing GENE QUANTIFICATION NEWSLETTER please contact gene.quantification@wzw.tum.de   You will receive all NEW FEATURES and future REST &  REST-XL  &  BestKeeper   updates.

GENE QUANTIFICATION web page and the GENE QUANTIFICATION NEWSLETTER are non-commercial, educational sites with the only purpose of facilitating access to biology-related information over the internet. The web page is optimised for Netscape Navigator version 4.7 & Internet Explorer version 5.0 (and higher versions) with an optimal screen resolution of 1024 x 768 pixels.

GENE QUANTIFICATION web page visits and DOWNLOAD statistics are listed here: http://www.wzw.tum.de/gene-quantification/contact.html= #server

If you want UN-SUBSCRIBE from all future NEWSLETTERS please contact gene.quantification@wzw.tum.de and type REMOVE into the subject line. You will be removed instantly from our mailing list.

********************************************************************
Gene Quantification @ wzw.tum.de

e-mail: gene.quantifi= cation@wzw.tum.de
homepage:       http://www.wzw.tum.de/gene-quantification/index.shtml
homepage:       http://www.wzw.tum.de/gene-quantification/physiology.shtml
homepage:       http://www.wzw.tum.de/gene-quantification/bioinformatics= .shtml

responsible Senior Scientist:
Dr. Michael W. Pfaffl
Institute of Physiology
Life Science Center Weihenstephan,
Technical University of Munich
Weihenstephaner Berg 3
D-85350 Freising-Weihenstephan
GERMANY

******************************************************************** --=====================_7835890==_.ALT-- ---