From owner-cfbs@net.bio.net Tue Jul 01 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Ashok Grover Newsgroups: bionet.prof-society.cfbs Subject: geometry and survival Date: 2 Jul 1997 05:48:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5pdin0$roe@net.bio.net> NNTP-Posting-Host: net.bio.net For July, we are discussing a paper on the possible role of substrate geometry in cell survival. Check out the ELECTRONIC JOURNAL CLUB IN PHARMACOLOGY web site http://www-fhs.mcmaster.ca/mcpharm/ From owner-cfbs@net.bio.net Wed Jul 09 23:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.prof-society.cfbs Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 10 Jul 1997 09:08:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5q31d3$qb1@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-cfbs@net.bio.net Wed Jul 09 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Ashok Grover Newsgroups: bionet.prof-society.cfbs Subject: Substrate shape and cell survival Date: 10 Jul 1997 09:08:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5q31e7$qk1@net.bio.net> NNTP-Posting-Host: net.bio.net Check out the ELECTRONIC JOURNAL CLUB IN PHARMACOLOGY web site http://www-fhs.mcmaster.ca/mcpharm/ From owner-cfbs@net.bio.net Wed Jul 09 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Justin Neway Newsgroups: bionet.prof-society.cfbs Subject: Fermentation Conference - Call for Posters Date: 10 Jul 1997 09:09:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 105 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5q31fr$qo4@net.bio.net> NNTP-Posting-Host: net.bio.net PLEASE POST The conference on Recent Advances in Fermentation Technology (RAFT II), will be held in San Diego, November 15 - 18, 1997. Oral presentations have now been finalized, and we are inviting poster presentations. If you and/or a colleague are interested in presenting a poster at the conference, please contact SIM headquarters (info@simhq.org) and request an abstract form. Please complete and return the form to SIM by September 1, 1997. Society for Industrial Microbiology 3929 Old Lee Highway, Ste. 92A Fairfax, VA 22030-2421, U.S.A. Tel: 703-691-3357 Fax: 703-691-7991 e-mail: info@simhq.org A listing of topic areas and other general information for the conference follows: Sessions and Convenors: Innovative Methods for Monitoring or Controlling Bioprocesses Convenors: Bing Miller, Fermtech, and Robert D. Schwartz, Ph.D., Abbott Laboratories Micro-gravity Fermentations Convenor: David Klaus, Ph.D., Aerospace Eng. Sciences, University of Colorado Strain Development for Improved Fermentation Properties Convenors: Carol A. Baker Ph.D., Pharmacia & Upjohn, Inc., and Alan Berry, Ph.D., BioTechnical Resources, Inc. Fermentation Optimization, Control and Process Improvement Convenors: Amit Vasavada, Ph.D., Kelco, and Konstantin B. Konstantinov, Ph.D., Bayer Corporation. Metabolic Engineering Convenors: Scott D. Power, PhD, Genencor International, and Prof. Lonnie O. Ingram, University of Florida. Cell Culture Convenors: Sadettin S. Ozturk, Ph.D., Bayer Corporation, and Geno Gramp, Ph.D., Amgen Corporation. Conference Chairs: Justin O. Neway, Ph.D., Vice President, Applications Aegis Analytical Corp, 3197 Nelson Road, Longmont, CO 80503 Tel & Fax: (303) 440-7561 e-mail: jneway@aegiscorp.com James C. Liao, Ph.D. Associate Professor, Department of Chemical Engineering Texas A&M University, College Station, TX 77843-3122 Tel: (409) 845-9807. Fax: (409) 845-6446 e-mail: j-liao@tamu.edu Keynote Speaker: Frederick C. Neidhardt, Ph.D., Acting Vice President for Research, University of Michigan Banquet Speaker: Steve C. Mendell, President & CEO, Prizm Pharmaceuticals Corporation Advisory Committee: Barry C. Buckland PhD, Vice-President, Bioprocess R&D, Merck Research Laboratories Prof. Arnold Demain, Professor of Industrial Microbiology, Department of Biology, MIT Prof. Michael Flickinger, Professor of Biochemistry, University of Minnesota Karl J. Sanford, PhD, Vice President, Technology Division, Genencor International Prof. Anthony J. Sinskey, Professor of Microbiology, Department of Biology, MIT Conference Deadlines: Poster abstracts due: Sept. 1 Final program information and poster order: Oct. 1 Programs will be mailed out: Oct. 27 ******************************************************* Justin O. Neway, Ph.D., VP, Applications Aegis Analytical Corporation 3197 Nelson Road, Longmont, CO 80503-9003 Tel: (303) 440-7561, Fax: (303) 450-4125 Knowledge-based Software for Regulated Manufacturers From owner-cfbs@net.bio.net Thu Jul 10 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Rathbun, Sapir & Associates" Newsgroups: bionet.prof-society.cfbs Subject: Position: Cell Culture Manufacturing, Supervisors [3] Date: 11 Jul 1997 09:02:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 43 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5q5lf4$pi4@net.bio.net> NNTP-Posting-Host: net.bio.net Cell Culture Manufacturing, Supervisors [3] We are seeking experienced BS/MS level Cell Culture Engineers/Scientists experienced in large-scale production of recombinant proteins for our biopharmaceutical client company=92s new manufacturing facility. This person will have ideally gone through the start up phase of a new manufacturing facility and must be familiar with the production of clinical materials. DESIRED PROFILE: =B7 BS/MS in Chemical/Biochemical Engineering or equivalent. =B7 4-7 years of industrial experience in: Cell culture and microbial process development Large-scale operations, minimum of 200 L GMP production of clinical materials. =B7 Prior supervisory experience. GENERAL JOB RESPONSIBILITIES: =B7 Supervise production of biopharmaceutical products for commercial use under cGMP conditions. =B7 Assist in the validations of the manufacturing facilities, including process validation studies. =B7 Supervise daily operations of the Cell Culture area; and work with facility staff in plant maintenance. =B7 Supervise reporting staff, assist in personnel training for manufacturing operations, assist in/contribute to the preparation of performance appraisals and recruitment of staff. =B7 Assist in budget planning. Communicate capital equipment needs, facility needs, and staffing needs; and contact service requirements. If you have an interest in this or other opportunities, please send us your resume/CV as an Attached File to an email or send by mail/FAX to RS&A to the attention of Ann G. Rathbun, Managing Director. All correspondence is held in strict confidence. Rathbun, Sapir & Associates P.O. Box 2337 Sedona, AZ 86339-2337 * USA (520) 284-3360 Office (520)284-3361 FAX E-mail: rathbun@ sedona.net From owner-cfbs@net.bio.net Thu Jul 10 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Justin Neway Newsgroups: bionet.prof-society.cfbs Subject: Fermentation Biotech Conference - Call for Posters Date: 11 Jul 1997 09:00:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 107 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5q5lbf$p75@net.bio.net> NNTP-Posting-Host: net.bio.net Fermentation Biotech Conference - Call for Posters PLEASE POST The conference on Recent Advances in Fermentation Technology (RAFT II), will be held in San Diego, November 15 - 18, 1997. Oral presentations have now been finalized, and we are inviting poster presentations. If you and/or a colleague are interested in presenting a poster at the conference, please contact SIM headquarters (info@simhq.org) and request an abstract form. Please complete and return the form to SIM by September 1, 1997. Society for Industrial Microbiology 3929 Old Lee Highway, Ste. 92A Fairfax, VA 22030-2421, U.S.A. Tel: 703-691-3357 Fax: 703-691-7991 e-mail: info@simhq.org A listing of topic areas and other general information for the conference follows: Sessions and Convenors: Innovative Methods for Monitoring or Controlling Bioprocesses Convenors: Bing Miller, Fermtech, and Robert D. Schwartz, Ph.D., Abbott Laboratories Micro-gravity Fermentations Convenor: David Klaus, Ph.D., Aerospace Eng. Sciences, University of Colorado Strain Development for Improved Fermentation Properties Convenors: Carol A. Baker Ph.D., Pharmacia & Upjohn, Inc., and Alan Berry, Ph.D., BioTechnical Resources, Inc. Fermentation Optimization, Control and Process Improvement Convenors: Amit Vasavada, Ph.D., Kelco, and Konstantin B. Konstantinov, Ph.D., Bayer Corporation. Metabolic Engineering Convenors: Scott D. Power, PhD, Genencor International, and Prof. Lonnie O. Ingram, University of Florida. Cell Culture Convenors: Sadettin S. Ozturk, Ph.D., Bayer Corporation, and Geno Gramp, Ph.D., Amgen Corporation. Conference Chairs: Justin O. Neway, Ph.D., Vice President, Applications Aegis Analytical Corp, 3197 Nelson Road, Longmont, CO 80503 Tel & Fax: (303) 440-7561 e-mail: jneway@aegiscorp.com James C. Liao, Ph.D. Associate Professor, Department of Chemical Engineering Texas A&M University, College Station, TX 77843-3122 Tel: (409) 845-9807. Fax: (409) 845-6446 e-mail: j-liao@tamu.edu Keynote Speaker: Frederick C. Neidhardt, Ph.D., Acting Vice President for Research, University of Michigan Banquet Speaker: Steve C. Mendell, President & CEO, Prizm Pharmaceuticals Corporation Advisory Committee: Barry C. Buckland PhD, Vice-President, Bioprocess R&D, Merck Research Laboratories Prof. Arnold Demain, Professor of Industrial Microbiology, Department of Biology, MIT Prof. Michael Flickinger, Professor of Biochemistry, University of Minnesota Karl J. Sanford, PhD, Vice President, Technology Division, Genencor International Prof. Anthony J. Sinskey, Professor of Microbiology, Department of Biology, MIT Conference Deadlines: Poster abstracts due: Sept. 1 Final program information and poster order: Oct. 1 Programs will be mailed out: Oct. 27 ******************************************************* Justin O. Neway, Ph.D., VP, Applications Aegis Analytical Corporation 3197 Nelson Road, Longmont, CO 80503-9003 Tel: (303) 440-7561, Fax: (303) 450-4125 Knowledge-based Software for Regulated Manufacturers From owner-cfbs@net.bio.net Thu Jul 10 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Donald Forsdyke Newsgroups: bionet.prof-society.cfbs Subject: test Date: 11 Jul 1997 09:00:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5q5lai$p66@net.bio.net> NNTP-Posting-Host: net.bio.net test test test From owner-cfbs@net.bio.net Thu Jul 10 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Rathbun, Sapir & Associates" Newsgroups: bionet.prof-society.cfbs Subject: Position: Cell Culture Manufacturing, Group Leader Date: 11 Jul 1997 09:02:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 52 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5q5lec$pgj@net.bio.net> NNTP-Posting-Host: net.bio.net Cell Culture Manufacturing, Group Leader We are seeking a PhD-level Cell Culture Engineer/Scientist experienced in the management of large-scale production of recombinant proteins for the Group Leader position in our biopharmaceutical client company=92s new manufacturing facility. This person will have ideally gone through the start up phase of a new manufacturing facility and must be familiar with the production of clinical materials. DESIRED PROFILE: =B7 Ph.D. in Chemical/Biochemical Engineering or equivalent. =B7 4-7 years of industrial experience in: Cell culture and microbial process development Large-scale operations, minimum of 200 L GMP production of clinical materials. =B7 Supervisory experience of 3+ BS/MS level techs. =B7 Solid hands-on experience with equipment, instrumentation, facility layout, etc. GENERAL JOB RESPONSIBILITIES: =B7 Manage production of biopharmaceutical products for commercial use under cGMP conditions. =B7 Work with Cell Culture staff to prepare MPRs, SOPs and other documents required for BLA submission. =B7 Provide process requirements and engineering insight in the design and construction of clinical manufacturing facilities. =B7 Assist in the validations of the manufacturing facilities. =B7 Manage daily operations of the Cell Culture area; and work with facility staff in plant maintenance. =B7 Design and carry out process validation studies. =B7 Represent Manufacturing on project team meetings. Take responsibility for cell culture effort. =B7 Supervise reporting staff, conduct personnel training for manufacturing operations, prepare performance appraisals, and recruit staff. =B7 Participate in budget planning. Communicate capital equipment needs, facility needs, and staffing needs; and contact service requirements. =B7 Participate in manufacturing operation and project team meetings. If you have an interest in this or other opportunities, please send us your resume/CV as an Attached File to an email or send by mail/FAX to RS&A to the attention of Ann G. Rathbun, Managing Director. All correspondence is held in strict confidence. Rathbun, Sapir & Associates P.O. Box 2337 Sedona, AZ 86339-2337 * USA (520) 284-3360 Office (520)284-3361 FAX E-mail: rathbun@ sedona.net From owner-cfbs@net.bio.net Thu Jul 10 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Rathbun, Sapir & Associates" Newsgroups: bionet.prof-society.cfbs Subject: Position: Cell Culture Process Dev't Group Leader Date: 11 Jul 1997 09:01:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 55 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5q5lcm$pc7@net.bio.net> NNTP-Posting-Host: net.bio.net Cell Culture/Fermentation Process Development Group Leader/Assoc. Director We are seeking a PhD-level Cell Culture Engineer/Scientist experienced in the development of large-scale processes for the production of recombinant proteins in prokaryotic and eukaryotic cells for our biopharmaceutical client company=92s Process Development Dept. This person will be responsible for the development of processes for production of materials for clinical trials under cGMP conditions; and, he/she will supervise Process Cell Culture/Fermentation Staff DESIRED PROFILE: =B7 Ph.D. in Chemical/Biochemical Engineering or equivalent. =B7 4-7 years of industrial experience in: Cell culture and, ideally, microbial process development Large-scale operations, minimum of 200 L GMP production of clinical materials. GENERAL JOB RESPONSIBILITIES: =B7 Design and carry out development strategies for large-scale cell culture processes.=20 =B7 Develop scaleable and robust bioreactor processes; transfer these processes to Manufacturing. =B7 Optimize bioreactor operating strategies to maximize product yield. =B7 Assist R&D staff in producing materials to support various research projects. =B7 Supervise and train laboratory staff in experimental design, interpretation, and planning. =B7 Maintain state-of-the-art expertise in technical areas and knowledge in supporting technical areas.=20 =B7 Manage production of biopharmaceutical products for clinical trials under cGMP conditions. =B7 Work with cell culture staff to prepare MPRs, SOPs and other documents required for IND/BLA submission. =B7 Provide process requirements and engineering insight in the design and construction of clinical manufacturing facilities. =B7 Assist in the validations of clinical manufacturing facilities, including the design and implementation of process validation studies. =B7 Manage daily operations of Clinical Manufacturing Area; and work with facility staff in plant maintenance. Participate in department and project team meetings; take responsibility for the cell culture effort. If you have an interest in this or other opportunities, please send us your resume/CV as an Attached File to an email or send by mail/FAX to RS&A to the attention of Ann G. Rathbun, Managing Director. All correspondence is held in strict confidence. Rathbun, Sapir & Associates P.O. Box 2337 Sedona, AZ 86339-2337 * USA (520) 284-3360 Office (520)284-3361 FAX E-mail: rathbun@ sedona.net From owner-cfbs@net.bio.net Thu Jul 17 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Paul Sully Newsgroups: bionet.prof-society.cfbs Subject: Japanese professor Date: 17 Jul 1997 17:20:24 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5qmcs8$7sq@net.bio.net> NNTP-Posting-Host: net.bio.net I'm posting this message for a friend of mine. He is a Japanese Professor at Osaka University of Health and sports Sciences. He wishes to do a sabbatical year, starting April 1st 1998, working on the effects of walking on the lipoprotein metabolism of vascular endothelium in dietary rats. He would like to study molecular biology and wishes to obtain a place in a laboratory, in Australia, at Japan's expense. If anybody could supply any information about Universities in Australia dealing in these areas or is able to make any suggestions, then I would be most grateful. Please reply directly to sully@gol.com. Thank you. From owner-cfbs@net.bio.net Thu Jul 17 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Mark Bisby Newsgroups: bionet.prof-society.cfbs Subject: Lecturer (Part-time) Date: 17 Jul 1997 17:19:23 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5qmcqb$7s6@net.bio.net> NNTP-Posting-Host: net.bio.net Department of Physiology Queen's University Half-Time Initial Lecturer Adjunct Position (20 h/week) The Lecturer's duties may include: Supervising teaching assistants and demonstrating in student laboratories; preparation of multimedia or distance educational materials; planning new courses or course revisions; lecturing or tutoring in undergraduate physiology courses. A Ph.D. in Physiology, or an M.Sc. and experience in teaching Physiology at the post-secondary level, is required. Experience in a teaching laboratory, or designing multimedia or computer-based instruction is desirable. This is a one-year, renewable, appointment, available September 1, 1997 or as soon as possible thereafter. Salary: up to $23,000 p.a. Please forward resume, and names of three referees, along with evidence of previous teaching experience, to Dr. M.A. Bisby, Department of Physiology, Queen's University, Botterell Hall, Room 442, Kingston, Ontario, K7L 3N6, by August 15, 1997. Queen's University is committed to employment equity and welcomes applications from all qualified men and women, including visible minorities, aboriginal people, persons with disabilities, gay men and lesbians. __________________________________________________________ Mark A. Bisby Department of Physiology Queen's University Kingston, Ontario, Canada. Phone: 613-545-2796 FAX: 613-545-6880 e-mail: bisbym@post.queensu.ca WWW: http://meds-ss10.meds.queensu.ca/medicine/physiol/bisby.htm From owner-cfbs@net.bio.net Thu Jul 17 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Ashok Grover Newsgroups: bionet.prof-society.cfbs Subject: Geometry and cell growth Date: 17 Jul 1997 17:21:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5qmcth$7tf@net.bio.net> NNTP-Posting-Host: net.bio.net What does the substrate geometry have to do with cell growth? Check out the ELECTRONIC JOURNAL CLUB IN PHARMACOLOGY web site http://www-fhs.mcmaster.ca/mcpharm/ From owner-cfbs@net.bio.net Thu Jul 17 23:00:00 1997 Path: biosci!biosci!not-for-mail From: JB Newsgroups: bionet.prof-society.cfbs Subject: HB-EGF as a protooncogene??? Date: 17 Jul 1997 17:21:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5qmcuu$7ud@net.bio.net> NNTP-Posting-Host: net.bio.net What=92s your opinion? Recently we have discussed in our lab if growth=20 factors like HB-EGF can be considered a=20 protooncogenes. To my opinion: =20 when considering the definition of a protooncogene is a gene=20 involved in proliferation and differentiation pathways, and when=20 mutated it causes in concert with other functionally related genes=20 a cancer, then HB-EGF could be also considered a protooncogene. From owner-cfbs@net.bio.net Sun Jul 27 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Michal Opas Newsgroups: bionet.prof-society.cfbs Subject: Calreticulin Meeting: 1st circular Date: 28 Jul 1997 09:24:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 323 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5rih33$krq@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Colleague, We are delighted to announce that Calreticulin Workshop,=20 devoted to the structure and function of calreticulin and=20 related proteins, will take place on March 31 - April 2,=20 1998 in Banff, Alberta, Canada. The Workshop will provide=20 unique opportunity to meet and interact with the scientists=20 interested in calreticulin research in spectacular=20 surroundings of Banff National Park in Canadian Rocky=20 Mountains. We are sure that the Banff Calreticulin Workshop=20 will be an important forum to share the latest findings and=20 to develop future interactions. Calreticulin has been=20 implicated to play a role in almost every aspect of cell=20 biology as outlined in a brief overview below. We hope that=20 the Workshop will be useful to sort out some of the latest=20 discoveries and controversies concerning calreticulin and=20 implication of this protein in a variety of biological=20 systems. On the behalf of the Organizing Committee we would=20 like to invite you to participate in the Workshop.=20 The Calreticulin Workshop is a satellite meeting to the 8th=20 Fisher Winternational Symposium on Cellular and Molecular=20 Biology which will be held April 2-5, 1998, also at the=20 Banff Conference Centre. The Winternational Symposium,=20 which is co-sponsored by our Society and Fisher Scientific,=20 is held annually, with a different focus each year. The=20 theme for the 1998 meeting is : "Membrane Proteins in Health=20 and Disease." Further information about the Fisher=20 Winternational or the satellite meetings can be obtained by=20 visiting the Society's web site at=20 http://www.csbmcb.ca/english/bulletin/winternational.e.html=20 or by contacting the Chair of the Scientific Program=20 Committee by E-mail: Carol Cass . I hope you will participate in the Calreticulin Workshop. =20 If you would like to receive further information please send=20 a request (if possible, by e-mail) to Michal Opas at: =09 m.opas@utoronto.ca =20 or at: =09Department of Anatomy & Cell Biology =09University of Toronto =09Medical Sciences Building =09Toronto, Ontario, M5S 1A8 Canada =09 tel: (416) 978-8947 =09 fax: (416) 978-3954 I look forward to hearing from you in the near future. For The Organizing Committee Sincerely yours Michal Opas Calreticulin, a multifunctional protein Calreticulin, 60 kDa Ca-binding protein [1], is a major=20 component of the endoplasmic reticulum (ER) of non-muscle=20 cells [2-7]. The protein is of high physiological=20 importance as it knockout is embryonic lethal [8]. Along=20 with a wide tissue distribution [9], calreticulin is present=20 in diverse animal and plant species [10]. calreticulin is a=20 resident ER protein as demonstrated by a variety of=20 biochemical and immunological techniques [1,3,4,6,11]. The=20 protein is synthesized with an N-terminal signal sequence=20 and it terminates with the KDEL sequence [3,12] which is=20 responsible for retrieval of proteins to the lumen of the ER=20 [13,14]. =20 =09Calreticulin functions in vivo as a Ca storage=20 protein [15,16]. It also has been well established that=20 calreticulin is a chaperone [17-21] and it shows similarity=20 in amino acid sequence to a part of calnexin, an ER membrane=20 chaperone [22]. The Ca storage and chaperone functions of=20 calreticulin are consistent with both the ER localization of=20 calreticulin and its structure. Stable overexpression of=20 calreticulin increases both cell-substratum and cell-cell=20 adhesiveness with concomitant upregulation of=20 adhesion-specific cytoskeletal protein, vinculin [23]. =20 Upregulation of calreticulin also affects adhesion-dependent=20 phenomena such as cell motility (which decreases) and cell=20 spreading (which increases). Downregulation of calreticulin=20 brings about inverse effects. In addition to the Ca=20 storage and chaperone function, calreticulin modulates gene=20 expression [24,25]. In vitro, calreticulin interaction with=20 the DNA binding domain of the glucocorticoid receptor=20 prevents the receptor from interacting with its=20 glucocorticoid response element [24]. Transcriptional=20 activation by glucocorticoid and androgen receptors in vivo=20 is inhibited in cells overexpressing full length=20 calreticulin [24,25]. Calreticulin itself is=20 stress-regulated by heat and heavy metals [26-28]. =20 Calreticulin has antithrombotic activity [29]. A host of=20 other putative calreticulin functions includes a role in=20 autoimmune diseases [30-34]. The protein affects=20 replication of the Rubella virus RNA [35,36]. In cytolytic=20 T lymphocytes it is found in the lytic granules where it may=20 play a role in killing of target cells [37]. In human=20 neutrophils calreticulin may contribute to the process of=20 phagocytosis [38]. In line with the reported functional=20 diversity, calreticulin was reported to be present in most=20 cellular compartments [10,11,37,39,40], including the outer=20 cell surface [41,42]. Recent hypotheses regarding=20 calreticulin function have been presented by Krause and=20 Michalak [43]. =09=09References 1. Ostwald TJ, MacLennan DH: Isolation of a high affinity=20 calcium binding protein from sarcoplasmic reticulum. J Biol=20 Chem 1974, 249:974-979. 2. Baksh S, Michalak M: Expression of calreticulin in=20 Escherichia coli and identification of its Ca2+ binding=20 domains. J Biol Chem 1991, 266:21458-21465. 3. Fliegel L, Burns K, Opas M, Michalak M: The=20 high-affinity calcium binding protein of sarcoplasmic=20 reticulum. Tissue distribution, and homology with=20 calregulin. Biochim Biophys Acta 1989, 982:1-8. 4. Opas M, Dziak E, Fliegel L, Michalak M: Regulation of=20 expression and intracellular distribution of calreticulin, a=20 major calcium binding protein of nonmuscle cells. J Cell=20 Physiol 1991, 149:160-171. 5. Milner RE, Baksh S, Shemanko C, Carpenter MR, Smillie L,=20 Vance JE, Opas M, Michalak M: Calreticulin, and not=20 calsequestrin, is the major calcium binding protein of=20 smooth muscle sarcoplasmic reticulum and liver endoplasmic=20 reticulum. J Biol Chem 1991, 266:7155-7165. 6. Michalak M, Baksh S, Opas M: Identification and=20 immunolocalization of calreticulin in pancreatic cells: no=20 evidence for "calciosomes". Exp Cell Res 1991, 197:91-99. 7. Michalak M, Milner RE, Burns K, Opas M: Calreticulin.=20 Biochem J 1992, 285:681-692. 8. Coppolino MG, Woodside MJ, Demaurex N, Grinstein S,=20 St-Arnaud R, Dedhar S: Calreticulin is essential for=20 integrin-mediated calcium signalling and cell adhesion.=20 Nature 1997, 386:843-847. 9. Tharin S, Dziak E, Michalak M, Opas M: Widespread tissue=20 distribution of rabbit calreticulin, a non-muscle functional=20 analogue of calsequestrin. Cell Tissue Res 1992, 269:29-37. 10. Opas M: The intracellular distribution and expression=20 of calreticulin. In Calreticulin, edited by Michalak M.=20 Georgetown: R.G. Landes; 1996:31-41. 11. Koch GLE: The endoplasmic reticulum and calcium=20 storage. BioEssays 1990, 12:527-531. 12. Fliegel L, Burns K, MacLennan DH, Reithmeier RAF,=20 Michalak M: Molecular cloning of the high affinity=20 calcium-binding protein (calreticulin) of skeletal muscle=20 sarcoplasmic reticulum. J Biol Chem 1989, 264:21522-21528. 13. Pelham HRB: Control of protein exit from the=20 endoplasmic reticulum. Annu Rev Cell Biol 1989, 5:1-23. 14. S=F6nnichsen B, F=FCllekrug J, Van PN, Diekmann W, Robinson=20 DG, Mieskes G: Retention and retrieval: Both mechanisms=20 cooperate to maintain calreticulin in the endoplasmic=20 reticulum. J Cell Sci 1994, 107:2705-2717. 15. Bastianutto C, Clementi E, Codazzi F, Podini P, De=20 Giorgi F, Rizzuto R, Meldolesi J, Pozzan T: Overexpression=20 of calreticulin increases the Ca2+ capacity of rapidly=20 exchanging Ca2+ stores and reveals aspects of their lumenal=20 microenvironment and function. J Cell Biol 1995,=20 130:847-855. 16. Liu N, Fine RE, Simons E, Johnson RJ: Decreasing=20 calreticulin expression lowers the Ca2+ response to=20 bradykinin and increases sensitivity to ionomycin in=20 NG-108-15 cells. J Biol Chem 1994, 269:28635-28639. 17. Nauseef WM, McCormick SJ, Clark RA: Calreticulin=20 functions as a molecular chaperone in the biosynthesis of=20 myeloperoxidase. J Biol Chem 1995, 270:4741-4747. 18. Wada I, Imai S, Kai M, Sakane F, Kanoh H: Chaperone=20 function of calreticulin when expressed in the endoplasmic=20 reticulum as the membrane-anchored and soluble forms. J Biol=20 Chem 1995, 270:20298-20304. 19. Nigam SK, Goldberg AL, Ho S, Rhode MF, Bush KT, Sherman=20 MY: A set of endoplasmic reticulum proteins possessing=20 properties of molecular chaperones includes Ca2+-binding=20 proteins and members of the thioredoxin superfamily. J Biol=20 Chem 1994, 269:1744-1749. 20. Otteken A, Moss B: Calreticulin interacts with newly=20 synthesized human immunodeficiency virus type 1 envelope=20 glycoprotein, suggesting a chaperone function similar to=20 that of calnexin. J Biol Chem 1996, 271:97-103. 21. Hebert DN, Foellmer B, Helenius A: Calnexin and=20 calreticulin promote folding, delay oligomerization and=20 suppress degradation of influenza hemagglutinin in=20 microsomes. EMBO J 1996, 15:2961-2968. 22. Bergeron JJM, Brenner MB, Thomas DY, Williams DB:=20 Calnexin: a membrane-bound chaperone of the endoplasmic=20 reticulum. Trends Biochem Sci 1994, 19:124-128. 23. Opas M, Szewczenko-Pawlikowski M, Jass GK, Mesaeli N,=20 Michalak M: Calreticulin modulates cell adhesiveness via=20 regulation of vinculin expression. J Cell Biol 1996,=20 135:1913-1923. 24. Burns K, Duggan B, Atkinson EA, Famulski KS, Nemer M,=20 Bleackley RC, Michalak M: Modulation of gene expression by=20 calreticulin binding to the glucocorticoid receptor. Nature=20 1994, 367:476-480. 25. Dedhar S, Rennie PS, Shago M, Leung-Hagesteijn C-Y,=20 Yang H, Filmus J, Hawley RG, Bruchovsky N, Cheng H, Matusik=20 RJ, Gigu=E8re V: Inhibition of nuclear hormone receptor=20 activity by calreticulin. Nature 1994, 367:480-483. 26. Nguyen TQ, Capra JD, Sontheimer RD: Calreticulin is=20 transcriptionally upregulated by heat shock, calcium and=20 heavy metals. Mol Immunol 1996, 33:379-386. 27. Dreher D, Vargas JR, Hochstrasser DF, Junod AF: Effects=20 of oxidative stress and Ca2+ agonists on molecular=20 chaperones in human umbilical vein endothelial cells.=20 Electrophoresis 1995, 16:1205-1214. 28. Conway EM, Liu L, Nowakowski B, Steiner-Mosonyi M,=20 Ribeiro SP, Michalak M: Heat shock-sensitive expression of=20 calreticulin. In vitro and in vivo up-regulation. J Biol=20 Chem 1995, 270:17011-17016. 29. Kuwabara K, Pinsky DJ, Schmidt AM, Benedict C, Brett J,=20 Ogawa S, Broekman MJ, Marcus AJ, Sciacca RR, Michalak M,=20 Wang F, Pan YC, Grunfeld S, Patton S, Malinski T, Stern DM,=20 Ryan J: Calreticulin, an antithrombotic agent which binds to=20 vitamin K-dependent coagulation factors, stimulates=20 endothelial nitric oxide production, and limits thrombosis=20 in canine coronary arteries. J Biol Chem 1995,=20 270:8179-8187. 30. Karska K, Tuckova L, Steiner L, Tlaskalova-Hogenova H,=20 Michalak M: Calreticulin--the potential autoantigen in=20 celiac disease. Biochem Biophys Res Commun 1995,=20 209:597-605. 31. Boehm J, Orth T, Van Nguyen P, S=F6ling H-D: Systemic=20 lupus erythematosus is associated with increased=20 auto-antibody titers against calreticulin and grp94, but=20 calreticulin is not the Ro/SS-A antigen. Eur J Clin Invest=20 1994, 24:248-257. 32. Zhu J, Newkirk MM: Viral induction of the human=20 autoantigen calreticulin. Clin Invest Med 1994, 17:196-205. 33. Ben-Chetrit E: The molecular basis of the SSA/Ro=20 antigens and the clinical significance of their=20 autoantibodies. Br J Rheumatol 1993, 32:396-402. 34. McCauliffe DP, Sontheimer RD: Molecular=20 characterization of the Ro/SS-A autoantigens. J Invest=20 Dermatol 1993, 100:73S-79S. 35. Atreya CD, Singh NK, Nakhasi HL: The rubella virus RNA=20 binding activity of human calreticulin is localized to the=20 N-terminal domain. J Virol 1995, 69:3848-3851. 36. Singh NK, Atreya CD, Nakhasi HL: Identification of=20 calreticulin as a rubella virus RNA binding protein. Proc=20 Natl Acad Sci USA 1994, 91:12770-12774. 37. Dupuis M, Schaerer E, Krause K-H, Tschopp J: The=20 calcium-binding protein calreticulin is a major constituent=20 of lytic granules in cytolytic T lymphocytes. J Exp Med=20 1993, 177:1-7. 38. Stendahl O, Krause K-H, Krischer J, Jerstrom P, Theler=20 JM, Clark RA, Carpentier JL, Lew DP: Redistribution of=20 intracellular Ca2+ stores during phagocytosis in human=20 neutrophils. Science 1994, 265:1439-1441. 39. Nakamura M, Moriya M, Baba T, Michikawa Y, Yamanobe T,=20 Arai K, Okinaga S, Kobayashi T: An endoplasmic reticulum=20 protein, calreticulin, is transported into the acrosome of=20 rat sperm. Exp Cell Res 1993, 205:101-110. 40. Dedhar S: Novel functions for calreticulin: =20 Interaction with integrins and modulation of gene=20 expression. Trends Biochem Sci 1994, 19:269-271. 41. White TK, Zhu Q, Tanzer ML: Cell surface calreticulin=20 is a putative mannoside lectin which triggers mouse melanoma=20 cell spreading. J Biol Chem 1995, 270:15926-15929. 42. Gray AJ, Park PW, Broekelmann TJ, Laurent GJ, Reeves=20 JT, Stenmark KR, Mecham RP: The mitogenic effects of the B =20 chain of fibrinogen are mediated through cell surface=20 calreticulin. J Biol Chem 1995, 270:26602-26606. 43. Krause K-H, Michalak M: Calreticulin. Cell 1997,=20 88:439-443. =20 =20 =20 =20 Dr. Michal Opas Department of Anatomy & Cell Biology University of Toronto 1 King's College Circle Medical Sciences Building Toronto, Ontario, M5S 1A8 Canada =20 phone: (416) 978-8947 fax: (416) 978-3954 e-mail: m.opas@utoronto.ca www homepage: http://www.utoronto.ca/anatomy/opas/start.htm=20 =20 From owner-cfbs@net.bio.net Sun Jul 27 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Justin Neway Newsgroups: bionet.prof-society.cfbs Subject: Fermentation Conference Presentation Needed Date: 28 Jul 1997 09:25:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 32 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5rih4u$l1p@net.bio.net> NNTP-Posting-Host: net.bio.net Oral presentation needed - PLEASE POST The SIM/ACS Special Conference on Recent Advances in Fermentation Technology (RAFT II), scheduled for November 15 - 18, 1997 in San Diego, USA, has had a cancellation of one of the oral presentations in the session on: Strain Development for Improved Fermentation Properties If you would like to give an oral presentation at this session, or if you have a suggestion about someone who might, please contact one of the session convenors listed below as soon as possible: Carol A. Baker Ph.D., Pharmacia & Upjohn, Inc. E-mail: CABAKER2@am.pnu.com Tel: (616) 833-9220, Fax: (616) 323-4241) or Alan Berry, Ph.D., BioTechnical Resources, Inc. E-mail: aberry@biotechresources.com Tel: (414)-684-5518 Fax: (414)-684-5519 Many thanks for your help - Justin ******************************************************* Justin O. Neway, Ph.D., VP, Applications Aegis Analytical Corporation 3197 Nelson Road, Longmont, CO 80503-9003 Tel: (303) 440-7561, Fax: (303) 450-4125 Knowledge-based Software for Regulated Manufacturers From owner-cfbs@net.bio.net Sun Jul 27 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Georgi P. Gladyshev" Newsgroups: bionet.prof-society.cfbs Subject: thermodynamics and life Date: 28 Jul 1997 09:26:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 89 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5rih8f$lg7@net.bio.net> NNTP-Posting-Host: net.bio.net Subject: Supramolecular chemistry and thermodynamics of biological evolution, Thermodynamics of origin of life, Macrothermodynamics, Self-assembly of supramolecular structures, Supramolecules and Assemblies, Adaptation, Aging Dear Colleagues, My Institute: URL http://www.biograph.comstar.ru/evolution/ The information in my URL is: Hello to all, I am seeking professional scientists who would like to understand (to comprehend) my thermodynamic theory of the Evolution of Living Beings. This theory is presented in work: G. P. Gladyshev, "Thermodynamic theory of the Evolution of Living Beings" (in Russian, Moscow, 1996). The work will be published by NOVA Sci. Publ., Inc., NY USA. I can send an additional information. For best understanding of the work it is very imported to have the experience in fields: classical physical chemistry, biophysical chemistry, chromatography, biological evolution, ontogenesis, aging, DSC of tissues, ... . Please, respond to e-mail: biograph@online.comstar.ru or academy@endeav.msk.su or mail: Academy of Creative Endeavors, 36, Novy Arbat, Moscow, 121205, Russia. Many thanks in advance! Georgi P. Gladyshev Professor of Physical Chemistry (Institute of Ecological Biophysical Chemistry of the Academy of Creative Endeavors, Laboratory of Thermodynamics and Macrokinetics of Non-equilibrium Systems of N.N.Semionov Institute of Chemical Physics of the Russian Academy of Sciences), Professor of the Chair of Biomedical Systems and Devices (N.E.Bauman Moscow State Technical University, 1975-1985) Summary This book is devoted to the physical theory of the biological evolution. The theory is based on macrothermodynamics, i.e., the hierarchic thermodynamics of complex systems. The results of the studies presented in the book allow one to state that the peculiarities of the evolution of living beings, as well as the peculiarities of the chemical evolution, can be explained without the concepts of the dynamical self-organization and the dissipative structures. According to the second law, the tendency of the evolution of biological systems on chemical and supramolecular levels can be determined by studying the effect of thermodynamical self-organization (self-assembly). The criterion for estimating the evolutionary development of supramolecular structures of biosystems (biotissues) is given by the variation of the specific Gibbs function of their formation. During the processes of ontogenesis, philogenesis, and biological evolution in general, the specific supramolecular component of the Gibbs function of a biosystem, that is quasi-closed thermodynamically and kinetically, tends to a relative minimum. The value of this minimum is a characteric of the given biosystem surrounded by the given environment. The non-stationary theoretical model presented in the monograph explains the reasons causing the variations in the chemical composition and structure of living beings in the course of ontogenesis, philogenesis, and the evolution in general. It also allows to find out the rules determining the variations in the composition and structure of a biosystem during its adaptation to the external conditions. At present, it seems that the most essential applications of the theory relate to the study of the living creatures behavior and to gerontology, pharmacology, nutrition. The book is written as an interdisciplinary monograph and is designed for a reader with university education. During the last decades, an opinion has widely spread that there is "the apparent contradiction between biological order and laws of physics - particularly the second law of thermodynamics". Besides, it is claimed that this contradiction "cannot be removed as long as one tries to understand living systems by the methods of equilibrium thermodynamics". The author of the present work states: if living systems are described in the framework of hierarchic equilibrium thermodynamics, this contradiction does not exist. Review of the work will appear in one of the next issues of Journal of Biological Physics. See also: G.P.Gladyshev, "Thermodynamic Direction of Biological Evolution: Model and Reality", Biology Bulletin, Vol. 23, No. 4, 1996, pp. 315-322. Translated from Izvestia Akademii Nauk, Seriya Biologicheskaya, No. 4, 1996, pp. 389-397. I have an suggestion: introduce to your Web site the section "Thermodynamics of Aging" . Sincerely, Georgi Gladyshev. From owner-cfbs@net.bio.net Wed Jul 30 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Vincent Duronio Newsgroups: bionet.prof-society.cfbs Subject: Post-doc position Date: 31 Jul 1997 13:11:34 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Approved: biocan@marcello.augustana.ab.ca Distribution: world Message-ID: <5rqrhm$c1a@net.bio.net> NNTP-Posting-Host: net.bio.net Applications are being accepted for a post-doctoral position to be filled in the fall, 1997 for a recent graduate from either a biochemistry, molecular biology, immunology, or cell biology program. The interests of the laboratory are centred upon the role of PI 3-kinase in various signal transduction pathways, particularly in hemopoietic cells stimulated with cytokines such as IL-3, IL-5, GM-CSF and IL-4. This group was one of the first to demonstrate the importance of PI 3-kinase in inhibition of apoptosis, and we are now delineating other signalling events that intersect with or regulate the same events as the PI 3-kinase pathway. Several potential projects can be pursued that will be dependent upon the expertise and interests of the successful applicant. Please send C.V., statement of research interests, and names of references to: Dr. Vincent Duronio U.B.C. Dept. of Medicine Jack Bell Research Centre 2660 Oak St. Vancouver, B.C., V6H 3Z6 FAX - 604-875-4497 email - vduronio@vanhosp.bc.ca Vincent Duronio, Ph.D. Tel. 604-875-4707 Dept. of Medicine, U.B.C. FAX 604-875-4497 Jack Bell Research Centre, Rm. 417 2660 Oak St. Vancouver, B.C., Canada, V6H 3Z6