From owner-biofilms@hgmp.mrc.ac.uk  Tue Apr 12 16:56:07 2005
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From: worshipnonebutallah@gmail.com ("worshipnonebutallah@gmail.com")
Subject: congo red agar
Date: 11 Apr 2005 13:44:44 +0100
Organization: http://groups.google.com
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could anyone tell me how are congo red agar plates made?


also, i'd appreciate it if someone could send me the following paper:

Freeman DJ,Falkiner FR,Keane CT. New method for detecting slime
production by coagulase-negative staphylococci. J Clin Pathol
1989;42:872-4.

i really need it and don't have access to it.

thanks in advance!

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From owner-biofilms@hgmp.mrc.ac.uk  Tue Apr 12 16:56:18 2005
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From: brix.harlem@gmail.com ("brix.harlem@gmail.com")
Subject: Flocculation Problem
Date: 12 Apr 2005 11:27:49 +0100
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Hallo!

I'm looking for a possibility to avoid the flocculation of Pseudomonas
putida in shaking flasks. I already tried adding tween 80 (50=B5l per
liter). No improvement could be ascertained.
At the moment I'm using a complex medium (Peptone and Meat-Extract),
soon I will use different minimal media.
What influences the flocculation?

Thank you in advance.

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From owner-biofilms@hgmp.mrc.ac.uk  Wed Apr 20 23:57:13 2005
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From: bob <bbruner@uclink4.berkeley.edu>
Subject:  Re: congo red agar
Date:  Tue, 19 Apr 2005 19:50:21 -0700
Organization: University of California, Berkeley
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On 12 Apr 2005 16:56:04 +0100, worshipnonebutallah@gmail.com
("worshipnonebutallah@gmail.com") wrote:

 >could anyone tell me how are congo red agar plates made?

It is not necessarily a standard name. So what it means is something
you need to check with your own relevant sources.

Google on
congo red agar
gives the following item:

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=3934141&dopt=Abstract

That link is to the abstract, and the paper itself is freely available
from there. But you will need to decide whether their congo red agar
plates are what you want. If you need to, explore the google search
more.


One good source of media info is the oxoid manual, at www.oxoid.com.
Your medium is not listed, unless I mistyped something.

 >
 >also, i'd appreciate it if someone could send me the following paper:
 >
 >Freeman DJ,Falkiner FR,Keane CT. New method for detecting slime
 >production by coagulase-negative staphylococci. J Clin Pathol
 >1989;42:872-4.

Their online archives do not go back that far. If you don't know
anyone with good access to the print journal, suggest you email the
authors.

bob


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From owner-biofilms@hgmp.mrc.ac.uk  Fri Apr 22 09:55:20 2005
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To: biofilms@net.bio.net
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From: worshipnonebutallah@gmail.com ("worshipnonebutallah@gmail.com")
Subject: Re: congo red agar
Date: 21 Apr 2005 10:57:05 +0100
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 > Their online archives do not go back that far. If you don't know
 > anyone with good access to the print journal, suggest you email the
 > authors.

This happens to be my problem presently. I don't know anyone who will
be ready to do this favour... and the author's email (or even airmail
address) is not there in the abstract.

This paper has been cited at many other places for a method for making
congo red agar plates. I hope I find someone who has access to the
hardcopy of this article~!

---


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From owner-biofilms@hgmp.mrc.ac.uk  Tue Apr 26 23:14:25 2005
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From: bob <bbruner@uclink4.berkeley.edu>
Subject:  Re: congo red agar
Date:  Sat, 23 Apr 2005 10:33:28 -0700
Organization: University of California, Berkeley
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On 22 Apr 2005 09:55:18 +0100, worshipnonebutallah@gmail.com
("worshipnonebutallah@gmail.com") wrote:

 > > Their online archives do not go back that far. If you don't know
 > > anyone with good access to the print journal, suggest you email the
 > > authors.
 >
 >This happens to be my problem presently. I don't know anyone who will
 >be ready to do this favour... and the author's email (or even airmail
 >address) is not there in the abstract.

This is often (though not always) resolvable, but it takes a little
effort. Worth learning how, because sometimes it is important to you.

The abstract may contain an email address. For one this old, maybe it
does not. But it should tell you where the authors are -- or at least
where they were at that time. Find the web site for their institution,
and look for info about them; this will often reveal an email address.

Of course, maybe they have moved. So look for more recent papers by
them. Use whatever resources seem appropriate, but looking for more
recent papers in PubMed is one approach. If the paper has multiple
authors, you may want to try to track down more than one. Top priority
is to find the one listed as the corresponding author, but sometimes
one of the other authors is easier to find. Most authors try to be
helpful; after all, they like that people want to read their articles.

When emailing an author, it is nice to briefly state why you are
interested in the paper, and to give your scientific (institutional)
affiliation, if relevant. Briefly -- a sentence or two. It makes them
feel good -- and also makes them aware that your interest is serious
enough that they should take the time to reply.

bob


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