From owner-bio-matrix@net.bio.net Tue Jan 02 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!newsfeed.internetmci.com!in2.uu.net!news.voicenet.com!philly226.voicenet.com!gheavner From: gheavner@voicenet.com (George A. Heavner) Newsgroups: bionet.molbio.bio-matrix Subject: Metaloproteinase Date: Tue, 2 Jan 1996 19:34:21 LOCAL Organization: Voicenet - Internet Access - (215)674-9290 Lines: 12 Message-ID: NNTP-Posting-Host: philly226.voicenet.com X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #4] I would like your help in identifying a commercially available metaloproteinase inhibitor. I have a series of experiments that I want to run in which I need to prevent a cell surface protein from being cleaved from the surface of a cell by a metaloproteinase. Specifically, I want to prevent membrane bound TNF from being cleaved and appearing in the supernatant. Does anyone have experience with a small molecule inhibitor that I could use? Thanks George A. Heavner Senior Director, Peptide R&D Centocor, Inc. From owner-bio-matrix@net.bio.net Thu Jan 04 22:00:00 1996 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!news.sprintlink.net!news.edge.net!news From: "Stephen Fortunato, MD" Newsgroups: bionet.molbio.bio-matrix Subject: Tissue viablity testing Date: 5 Jan 1996 18:48:24 GMT Organization: The Edge Lines: 7 Message-ID: <4cjrpo$b4b@excalibur.edge.net> NNTP-Posting-Host: ip159.nash.edge.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 16bit) Is there any good methods available to test the viability of cells in an organ explant system (tissue culture and not cell culture). I have performed PLC assay on tisuue homogenate using PNPC (PN phosphoryl choline ) as substrate. we were succesfull in doing the experiments but unable to interpret the data we have. If someone knows this method or any other method please post a comment or reference in this section. From owner-bio-matrix@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!PUBLIC.BTA.NET.CN!wfchen From: wfchen@PUBLIC.BTA.NET.CN (user 3046) Newsgroups: bionet.molbio.bio-matrix Subject: (none) Date: 8 Jan 1996 03:21:55 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601081119.TAA02117@public.bta.net.cn> NNTP-Posting-Host: net.bio.net mRNA Strucrure Prediction Internet-Service Dear Experts: Please permit me to ask a naive question. We are just probing into the area of structural biology from the field of molecular immunology! We want to know the 2nd especially 3rd mRNA structure from our mRNA sequence. Computer modelling is just enough! The result is very meaningful to us! The OBSTACLE we are facing: our computer is not power enough. ARE THERE any e-mail server on the Internet to do that just as 'PredictProtein@EMBL-Heidelberg.DE'? OR, SHOULD YOU permit me use your mainframe to do this computation (I send you the seq. via e-mail)? I deem it a great honor to receive your early reply. Please reply personally to wfchen@public.bta.net.cn =-=-=-=-=-=-=-=-=-=-=-=-=- MANY THANKS! -=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Simon M. Lin Department of Immunology Phone: +86 10 593 4831 (24 hrs) Beijing Medical University CHINA "Not quite the third world" =-=-=-=-==-=-=-=-=-=-="Computer + Molecular Biology = Discovery !" -=-=-=-= From owner-bio-matrix@net.bio.net Mon Jan 08 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!vixen.cso.uiuc.edu!uwm.edu!caen!kuhub.cc.ukans.edu!kuhub.cc.ukans.edu!nntp Newsgroups: bionet.molbio.bio-matrix Subject: (no subject) Message-ID: <1996Jan8.174050.111440@kuhub.cc.ukans.edu> From: xiaoke zhang Date: 8 Jan 96 17:40:50 CST Organization: Kansas University Medical Center Nntp-Posting-Host: xzhang.schmed.kumc.edu X-Mailer: Mozilla 1.1N (Windows; I; 16bit) MIME-Version: 1.0 Content-Transfer-Encoding: 7bit Content-Type: text/plain; charset=us-ascii Lines: 14 Hello, everyone: I am a post-doctoral fellow in Kansas University Medical Center. I am now working with a project studying the relationships between adhesion and macrophage functions. I am looking for materials which I can use to coat tissue culture plates in order to make these plates NON-adhesible by macrophages. Anyone who has relevant information please e-mail to me at: xzhang@kumc.edu Thanks. Charlotte From owner-bio-matrix@net.bio.net Mon Jan 08 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!usenet.eel.ufl.edu!usenet.cis.ufl.edu!caen!kuhub.cc.ukans.edu!kuhub.cc.ukans.edu!nntp Newsgroups: bionet.molbio.bio-matrix Subject: nonadhesion materials Message-ID: <1996Jan8.174121.111441@kuhub.cc.ukans.edu> From: xiaoke zhang Date: 8 Jan 96 17:41:20 CST Organization: Kansas University Medical Center Nntp-Posting-Host: xzhang.schmed.kumc.edu X-Mailer: Mozilla 1.1N (Windows; I; 16bit) MIME-Version: 1.0 Content-Transfer-Encoding: 7bit Content-Type: text/plain; charset=us-ascii Lines: 14 Hello, everyone: I am a post-doctoral fellow in Kansas University Medical Center. I am now working with a project studying the relationships between adhesion and macrophage functions. I am looking for materials which I can use to coat tissue culture plates in order to make these plates NON-adhesible by macrophages. Anyone who has relevant information please e-mail to me at: xzhang@kumc.edu Thanks. Charlotte From owner-bio-matrix@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.bio-matrix Subject: BIOSCI miniFAQ, ver. 14-DEC-95 Date: 15 Jan 1996 02:00:21 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 199 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601151000.CAA15522@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 14-DEC-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index in addition to the master index for the entire set. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-bio-matrix@net.bio.net Tue Jan 16 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!swrinde!sdd.hp.com!col.hp.com!csn!csn!nntp-xfer-2.csn.net!yuma!purdue!haven.umd.edu!news.ums.edu!crick.sura.net!snooze.ser.bbnplanet.com!news.mountain.net!Access.Mountain.Net!rhterry From: rhterry@Access.Mountain.Net (Bob Terry) Newsgroups: bionet.molbio.bio-matrix Subject: MOREplus a WWW cataloging and database tool Date: 16 Jan 1996 20:36:09 GMT Organization: MountainNet, Inc. Morgantown WV 800.444.1458 Lines: 23 Message-ID: <4dh27p$igp@news.mountain.net> NNTP-Posting-Host: access.mountain.net X-Newsreader: TIN [version 1.2 PL2] Build and Maintain Complex Web-based Retrieval Systems for High-Value Information Unlike many Web search engines, and database forms generation tools, MOREplus is built for information assets that have high value to customers -- customers who don't have much time to muddle through long lists of assets. This need arises as organizations seek to promote and distribute proprietary and mission critical information across traditional boundaries. Corporate best practice descriptions, metrics data, or software can all be examples of high-value information assets. MOREplus provides a lot more than an Internet search engine. It enables you to meet a broad set of constraints and demands that are imposed on suppliers of such high-value assets -- without hiring a cadre of Web gurus. MOREplus is a World Wide Web-based cataloging and database tool. MOREplus allows you to develop, deploy and maintain complex information retrieval systems without writing Common Gateway Interface-based (CGI) scripts or developing databases. MOREplus can manage a broad range of file types that are located anywhere on the Web. Come and visit at: http://rbse.mountain.net/MOREplus From owner-bio-matrix@net.bio.net Wed Jan 17 22:00:00 1996 Path: biosci!rutgers!gatech!newsxfer2.itd.umich.edu!caen!kuhub.cc.ukans.edu!kuhub.cc.ukans.edu!nntp Newsgroups: bionet.molbio.bio-matrix Subject: teflon plates Message-ID: <1996Jan18.105219.112161@kuhub.cc.ukans.edu> From: xiaoke zhang Date: 18 Jan 96 10:52:18 CST Organization: Kansas University Medical Center Nntp-Posting-Host: xzhang.schmed.kumc.edu X-Mailer: Mozilla 1.1N (Windows; I; 16bit) MIME-Version: 1.0 Content-Transfer-Encoding: 7bit Content-Type: text/plain; charset=us-ascii Lines: 10 Hi, Everyone: I am working with a project studying adhesion phenomena. Does anyone know a source/company making teflon-coated tissue culture plates? Please response to: xzhang@kumc.edu Thanks. Charlotte From owner-bio-matrix@net.bio.net Wed Jan 17 22:00:00 1996 Newsgroups: bionet.general,bionet.info-theory,bionet.molbio.bio-matrix,bionet.molbio.evolution,bionet.molbio.genbank,bionet.software Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!portal.gmu.edu!hearst.acc.Virginia.EDU!murdoch!usenet From: wrp@alpha0.bioch.virginia.edu (William R. Pearson) Subject: Identifying Features in Biological Sequences Workshop, Aspen, June '96 X-Nntp-Posting-Host: alpha0.bioch.virginia.edu Message-ID: Followup-To: bionet.software Sender: wrp@alpha0.bioch.Virginia.EDU Organization: University of Virginia X-Newsreader: Gnus v5.1 Date: Thu, 18 Jan 1996 20:43:48 GMT Lines: 104 Xref: biosci bionet.general:19424 bionet.info-theory:3870 bionet.molbio.bio-matrix:699 bionet.molbio.evolution:4107 bionet.molbio.genbank:2208 bionet.software:14487 Dear Colleague: I am writing to inform you of our plans for this year's workshop at the Aspen Center for Physics on Identifying Features in Biological Sequences. This workshop, the seventh in the series, runs from 27 May to 16 June. The deadline for electronic applications (http://andy.bu.edu/aspen/application) is February 1; mail-in applications for this workshop must be received by February 15. While we prefer that participants spend the entire three weeks, we recognize that there are conflicts with this year's ISMB96 meeting and stays of two weeks can be acommodated. Our goal is to bring together about 25 researchers, from diverse backgrounds of biology, mathematics, computer science and other disciplines, that are interested in the problems of extracting biological information from nucleic acid and protein sequences. The workshop is designed to facilitate interactions and collaborations. Each participant has an office shared with one other participant. There will be a small network of Unix workstations available with connections to the Internet. The workshop's formal schedule is decided by the participants at the beginning of each week, but it usually involves a limited amount of time in formal talks, perhaps 2-3 hours each day, and the rest of the time available for small group interaction and collaboration. These often involve the development and testing of new methods, or the testing of old methods on new data and the comparison of different methods on standardized data sets. These collaborative interactions are the real strength of the Aspen workshops. A summary (postscript file) of the 1995 session can be downloaded from: ftp://atlas.lanl.gov/pub/cb/ACP95.ps. A printed copy can be obtained by sending email with your postal address, to: cb@t10.lanl.gov. Housing in Aspen is arranged through the Center and is very reasonable, making it possible to have family members join you to ameliorate the prospect of being absent for such an extended period. We have are pursuing funding which may partially offset housing costs and some travel expenses. If you would like to attend but need financial assistance to do so, I recommend that you apply before the deadline and then later decline the invitation if it becomes necessary. We will try to find funding for those that are accepted to the workshop and require it in order to attend. Attendance at the Center's workshops is usually well oversubscribed. If you want to come you must complete and return the application form so that it is received before 15 February (there are no exceptions). There are two options for applying: The first, and preferred, option is to apply electronically. ELECTRONIC APPLICATIONS MUST BE SENT BY FEBRUARY 1. The Center has set up a World Wide Web address that you can use for this purpose: http://andy.bu.edu/aspen/application. (Information about the Center and more information about its summer program can be found at http://andy.bu.edu/aspen). The WWW application process will not work after February 1. If electronic application is inconvenient or impossible, the second option is to complete a hardcopy application form (included here if you're receiving this note by postal service, and available by faxing a request to me at 804-924-5069 if you're receiving this note by e-mail), and sending it to: Sally Mencimer Aspen Center for Physics P.O. Box 1208 Aspen, CO 81612 tel: 303-925-2585 fax: 303-920-1167 email: mencimer@acp1.infosphere.com In either case, you should specify the workshop title and weeks (or subset thereof) -- as listed in the first paragraph above -- on the application so the Center will know it's our workshop in which you are interested. After 15 February, the Center's Admissions Committee, with input from our workshop's Organizing Committee (Christian Burks, Gene Myers, and myself), will select participants based on getting a broad representation of work in the field and a mixture of previous and new participants. Should some of those invited decline, more invitations will be extended later. We look forward to an exciting, productive workshop and encourage you, or someone in your group, to apply. Please feel free to post or pass this note along to anyone who might be interested. If you have any questions about our workshop please contact me by e-mail (wrp@virginia.edu); if you have questions about the Center's program and administrative details in general, please contact Sally Mencimer at the address above. Sincerely yours, William R. Pearson telephone: 804-924-2818 fax: 804-924-5069 e-mail: wrp@virginia.edu Dept. of Biochemistry Jordan Hall #440 University of Virginia Charlottesville, VA 22908 From owner-bio-matrix@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!QUERCUS.INBIO.AC.CR!mmora From: mmora@QUERCUS.INBIO.AC.CR (Maria auxiliadora Mora) Newsgroups: bionet.molbio.bio-matrix Subject: susbcribe Date: 18 Jan 1996 16:07:56 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601190005.AA00743@quercus.inbio.ac.cr> NNTP-Posting-Host: net.bio.net susbcribe bio-matrix _________________________________________ Maria Auxiliadora Mora Cross Instituto Nacional de Biodiversidad (INBio) Tel.(506)244-0690 - Fax.(506)244-2816 Apartado Postal 22-3100 Heredia, Costa Rica __________________________________________