From owner-biophysics@net.bio.net Sun Oct 01 23:00:00 1995 Path: biosci!daresbury!bioftp.unibas.ch!citi2.fr!jussieu.fr!oleane!tank.news.pipex.net!pipex!in1.uu.net!noc.near.net!usenet.uchc.edu!usenet From: Les Loew Newsgroups: bionet.biophysics Subject: CBIT Software Date: 2 Oct 1995 13:10:43 GMT Organization: Univ of CT Health Center Lines: 36 Message-ID: <44oocj$m2n@threed.uchc.edu> NNTP-Posting-Host: volt.uchc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; IRIX 5.3 IP22) X-URL: news:bionet.biophysics Dear Biophysicists, The Center for Biomedical Imaging Technology has available a number of SGI software applications that have been developed for use with our Zeiss and Biorad confocal microscopes as well as a number of digtal imaging widefield systems. We felt that these may be of use to others and are now making them available via anonymous ftp. They include: RatioView: a simple image analysis package that is especially convenient for calcium, pH, or membrane potential distribution in single cells by dual wavelength ratio imaging. A tutorial is included in our web site. CBITRender: a 3D visualization environment including a raytracer. Both single processor and distributed multiprocessor (running under LINDA) versions are available. Imgview3D: utilities for creating and visualizing 3D datasets with the SGI Imagevision library. BioRad Library: an extension to the ImageVision Library permitting it to automatically read the BioRad .pic fileformat. The software can all be found on the web at http://panda.uchc.edu/htbit/ and following the thread under "Imaging Research and Development". While you are there, please learn about the other activities at CBIT. Les Loew Professor of Physiology Director, Center for Biomedical Imaging Technology University of Connecticut Health Center Farmington, CT 06030-1507 Tel. 203-679-3568 FAX 203-679-1269 Best E-mail les@volt.uchc.edu CBIT URL http://panda.uchc.edu/htbit/ From owner-biophysics@net.bio.net Sun Oct 01 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!swrinde!tank.news.pipex.net!pipex!newsxfer.itd.umich.edu!news.itd.umich.edu!usenet From: Rick Neubig Newsgroups: bionet.biophysics Subject: Re: Curve Fitting Date: 2 Oct 1995 18:59:57 GMT Organization: University of Michigan Lines: 31 Message-ID: <44pcrd$mar@lastactionhero.rs.itd.umich.edu> References: <44hddf$58o@news.ycc.yale.edu> NNTP-Posting-Host: warbler.med.umich.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: Ujwal@trna.chem.yale.edu,hmotulsky@graphpad.com "M.L. UJWAL" wrote: >Hello, > I would appreciate suggestions finding a BOOK/MANUAL >explaining all the nitty-gritty details about CURVE FITTING >data, especially in the experimental areas of (1) Receptor >Agonist/Antagonist binding (2) Pre-steady state kinetics of >Enzyme catalysis using Stopped-Flow measurements. > Sincerely > > UJWAL Hi, There is information (several chapters in Microsoft Word format) which you can download from the GraphPad Software home page which includes extensive discussions on fitting of Receptor Agonist/Antagonist binding data. See: http://www.graphpad.com Go to the link on "guides to nonlinear regression, analysis of radioligand binding data, or statistical comparisons". Disclaimer - I consult for them so this is for general information only. _________________________________________________________ Rick Neubig RNeubig@umich.edu University of Michigan Phone (313) 763-3650 http://www.umich.edu/~rneubig FAX (313) 763-4450 From owner-biophysics@net.bio.net Sun Oct 01 23:00:00 1995 Path: biosci!daresbury!bioftp.unibas.ch!citi2.fr!jussieu.fr!oleane!tank.news.pipex.net!pipex!uknet!bhamcs!news.ox.ac.uk!news From: "Simon M. Brocklehurst" Newsgroups: bionet.biophysics Subject: NAOMI Version 2.2 Date: 2 Oct 1995 13:54:30 GMT Organization: University of Oxford Lines: 68 Message-ID: <44oqum$ju5@news.ox.ac.uk> NNTP-Posting-Host: nmrz.ocms.ox.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; IRIX 5.3 IP22) X-URL: news:bionet.biophysics NAOMI - Version Upgrade Announcement (Please note, NAOMI is provided at zero charge for academic use) (e-mail contact smb@bioch.ox.ac.uk) _____________________________________________________________________________ The computer program NAOMI Version 2.2 is available _now_ from the NAOMI Web site at: http://www.ocms.ox.ac.uk/~smb/Software/N_details/naomi.html or via anonymous ftp from: nmrz.ocms.ox.ac.uk in directory pub/smb/naomi/ Users of versions older than 2.10 will need new license keys to allow the upgrade to work (please contact the author in this case). _____________________________________________________________________________ Upgrade features : solvent accessibility, symmetry operations, disulphide bonds, new side-chain modelling commands, new molscript output options e.g. see the illustrations on the Cytokines Web: http://www.ocms.ox.ac.uk/~smb/cyt_web/ Fixes of minor bugs in key residues and molscript output commands. _____________________________________________________________________________ What is NAOMI? NAOMI is an easy-to-use, state-of-the-art computer program which is aimed at both specialist and non-specialist researchers who make use of three-dimensional structures of proteins in their work. It has hundreds of users Worldwide. Some facilities offered by the program for working with structure include: automatic 'key' residue identification automatic hydrophobic core/packing analysis automatic hydrogen bonds main-chain and side-chain identification (including high quality energy calculations) automatic secondary structure (helix, strand and turn) classification using fuzzy logic automatic supersecondary structure classification (beta-hairpin loops) conformational parameters: phi,psi,chi1,chi2,chi3,chi4,chi5 etc solvent accessibility (both absolute and percentage) calculations automatic identification of disulphide bonds, salt bridges, chain-breaks side-chain modelling and manipulation applying symmetry operators automatic structure repair (building in missing atoms) NMR structure refinement module interfaces to graphics programs (MOLSCRIPT (and thus Raster3D), INSIGHT, QUANTA to allow automatic preparation of figures More details are available on the Web site. NB NAOMI currently works only on Silicon Graphics workstations running IRIX 5.* _____________________________________________________________________________ | | ,_ o Simon M. Brocklehurst, | / //\, Oxford Centre for Molecular Sciences, Department of Biochemistry, | \>> | University of Oxford, Oxford, UK. | \\, E-mail: smb@bioch.ox.ac.uk | WWW: http://www.ocms.ox.ac.uk/~smb/ |____________________________________________________________________________ From owner-biophysics@net.bio.net Mon Oct 02 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!news00.sunet.se!sunic!sunic!sunic.sunet.se!news.uni-c.dk!unidhp.uni-c.dk!tycho.gbar.dtu.dk!fimm From: fimm@tycho.gbar.dtu.dk (Martin Manscher (c929001)) Newsgroups: bionet.biophysics Subject: Textbook on Physiology Date: 3 Oct 1995 15:53:37 GMT Organization: DTU Lines: 13 Message-ID: <44rma1$ebv@unidhp.uni-c.dk> NNTP-Posting-Host: tycho.gbar.dtu.dk X-Newsreader: TIN [version 1.2 PL1] Hi! I am taking a basic/elementary course in Physiology, and the recommended book is the worst textbook i have ever seen. Could anyone please recommend a comprehensive textbook including all the basics off physiology? (I don't know if this is the right place to post this, but there was no bionet.physiology newsgroup) Yours sincerely, Martin Manscher (fimm@gbar.dtu.dk) From owner-biophysics@net.bio.net Mon Oct 02 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!spool.mu.edu!bloom-beacon.mit.edu!senator-bedfellow.mit.edu!athena.mit.edu!tvaughan From: tvaughan@athena.mit.edu (Timothy E Vaughan) Newsgroups: bionet.biophysics Subject: Re: Textbook on Physiology Date: 3 Oct 1995 21:14:26 GMT Organization: Massachusetts Institute of Technology Lines: 9 Distribution: world Message-ID: <44s93i$eu9@senator-bedfellow.MIT.EDU> References: <44rma1$ebv@unidhp.uni-c.dk> NNTP-Posting-Host: milanese.mit.edu Maybe this is the book you are using (and hate), but the classic text is Arthur Guyton's "Textbook of Medical Physiology." I like it, but I haven't really looked at any others in direct comparison. Cheers, Tim From owner-biophysics@net.bio.net Tue Oct 03 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!xlink.net!news.dfn.de!scsing.switch.ch!rzunews.unizh.ch!NewsWatcher!user From: maga@vetbio.unizh.ch (Giovanni Maga) Newsgroups: bionet.biophysics Subject: Re: Curve Fitting Date: Wed, 04 Oct 1995 08:37:37 -0500 Organization: University of Zurich Irchel- Biochemistry Lines: 22 Message-ID: References: <44hdc3$58o@news.ycc.yale.edu> NNTP-Posting-Host: 130.60.120.11 In article <44hdc3$58o@news.ycc.yale.edu>, "M.L. UJWAL" wrote: > Hello, > I would appreciate suggestions finding a BOOK/MANUAL > explaining all the nitty-gritty details about CURVE FITTING > data, especially in the experimental areas of (1) Receptor > Agonist/Antagonist binding (2) Pre-steady state kinetics of > Enzyme catalysis using Stopped-Flow measurements. I would suggest to read the excellent work of K.A. Johnson on pre-steady state kinetic analysis of enzymes by using rapid quenching techniques. It's in Methods in Enzymology, 249 pp38-61. The following chapter by R.G.Duggleby is about analysis of enzyme progress curves by non-linear regression, that could be also of interest for you. The same issue contains more articles about enzyme kinetics and I would suggest to get your hands also on the other volumes of the same series about Enzyme Kinetics and Mechanism, where you could find several papers concerning the best aprroach to fitting data to rate equations. Hope it helps. Giovanni. From owner-biophysics@net.bio.net Tue Oct 03 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!EU.net!sun4nl!news.nic.surfnet.nl!news.nic.utwente.nl!news From: "Zeger" Newsgroups: bionet.biophysics Subject: Re: Textbook on Physiology Date: Wed, 4 Oct 95 11:15:27 CST Organization: University of Twente, Enschede, The Netherlands Lines: 25 Message-ID: <53562.s9516042@mail.student.utwente.nl> Reply-To: NNTP-Posting-Host: pc003.pczaalciv.utwente.nl X-Minuet-Version: Minuet1.0_Beta_17 X-POPMail-Charset: English To: tvaughan@athena.mit.edu On 3 Oct 1995 21:14:26 GMT, Timothy E Vaughan wrote: >Maybe this is the book you are using (and hate), but the classic >text is Arthur Guyton's "Textbook of Medical Physiology." >I like it, but I haven't really looked at any others in direct >comparison. > >Cheers, > >Tim > > Try "sein und seit" from Heidegger. Much more fun. z.f.knops@student.utwente.nl From the "Shockwave Rider" "First there was the legs race, Then there was the arms race, Then came the brain race" From owner-biophysics@net.bio.net Tue Oct 03 23:00:00 1995 Path: biosci!bcm.tmc.edu!pendragon.jsc.nasa.gov!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!howland.reston.ans.net!swrinde!sdd.hp.com!hamblin.math.byu.edu!news.Arizona.EDU!usenet From: Soaring Bear Newsgroups: bionet.biophysics,bionet.toxicology,bionet.xtallography Subject: Biosym's Apex QSAR program Date: 4 Oct 1995 23:21:27 GMT Organization: The University of Arizona Lines: 27 Message-ID: <44v4tn$pda@news.ccit.arizona.edu> NNTP-Posting-Host: basie.pharm.arizona.edu Mime-Version: 1.0 X-Mailer: Mozilla 1.1N (X11; I; IRIX 5.2 IP22) Xref: biosci bionet.biophysics:1290 bionet.toxicology:198 bionet.xtallography:2063 References: <53562.s9516042@mail.student.utwente.nl> X-URL: news:53562.s9516042@mail.student.utwente.nl Content-Transfer-Encoding: 7bit Content-Type: text/plain; charset=us-ascii Hello: Despite my posts to CCL & Dibug I have been unable to find a single satisfied user of Biosym's Apex QSAR software. I have received several emails from folks who tried and gave up. Is there anyone out there who is successfully using it? who has published? Please email me at: bear@ellington.pharm.arizona.edu thankyou -- _____ ____ * ___) * _ \ http://ellington.pharm.arizona.edu/~bear : (___ : |_) : Cyber-Chemist: cancer drug design topo-O O-topo \___ \ | _ < Molecular & Nutritional Biochemist 5'*. : : .***. ___) : | |_) : Herbs, Nutrition, Natural Dentistry | *. .* | | | (_____/oaring |____/ear@ellington.pharm.arizona.edu | | *. * | | | * UA New Pharmacy 404, Tucson 85721 3'.| DNA helix| * '***' '***' From owner-biophysics@net.bio.net Thu Oct 05 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!tank.news.pipex.net!pipex!wave.news.pipex.net!pipex!dish.news.pipex.net!pipex!news00.sunet.se!sunic!news.sprintlink.net!in2.uu.net!newsfeed.pitt.edu!dsinc!ub!acsu.buffalo.edu!ab4 From: ab4@acsu.buffalo.edu (Anindya Bhattacharya) Newsgroups: bionet.biophysics Subject: fellowship Date: 6 Oct 1995 17:10:43 GMT Organization: UB Lines: 9 Message-ID: <453nuj$d5f@azure.acsu.buffalo.edu> NNTP-Posting-Host: lictor.acsu.buffalo.edu NNTP-Posting-User: ab4 X-Newsreader: TIN [version 1.2 PL2] Hello : I am a third year doctoral student in pharmacology at SUNY Buffalo, USA. I am working on the electrophysiology of ion channels in Drosophila. I am in need of fellowships to support my graduate education. My choices becomes too limited because I am not a US citizen nor am I a permanent resident (green card). Any responses will be appreciated. Anindya From owner-biophysics@net.bio.net Thu Oct 05 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.sprintlink.net!howland.reston.ans.net!newsjunkie.ans.net!inet.d48.lilly.com!sherwood.d46.lilly.com!gfn Newsgroups: bionet.biophysics Subject: Job Available Message-ID: <1995Oct6.131334.6442@inet.d48.lilly.com> From: Greg Needham Date: 6 Oct 95 13:13:34 EST Distribution: world Organization: Eli Lilly and Co. Nntp-Posting-Host: sherwood.d46.lilly.com X-UserAgent: Version 1.1.3 X-XXMessage-ID: X-XXDate: Fri, 6 Oct 95 13:14:15 GMT Lines: 17 Associate Formulations Chemist. An associate formulations chemist is needed for the development and characterization of formulations for new animal health products. This scientist will provide laboratory support for the identification of new dosage forms or delivery systems for synthetic organic compounds and fermentation products. Preference will be given to qualified candidates having a BS or MS degree and relevant experience in physical chemistry, chemical engineering, pharmaceutics, pharmaceutical sciences, or a related field. Interested individuals should please respond with a resume to: Mr. Doug Whiteman Lilly Research Laboratories 2001 West Main Street Greenfield, IN 46140 Eli Lilly and Company is an equal opportunity employer. From owner-biophysics@net.bio.net Thu Oct 05 23:00:00 1995 Path: biosci!UCRWCU.RWC.UC.EDU!koehlekr From: koehlekr@UCRWCU.RWC.UC.EDU (Ken Koehler) Newsgroups: bionet.biophysics Subject: electrophoresis Date: 6 Oct 1995 13:00:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510061959.MAA04026@net.bio.net> NNTP-Posting-Host: net.bio.net In "Physical Chemistry", Tinoco, Sauer & Wang (3rd Ed.,1995), page 305, I read ", the relation log M = a - b x is usually observed." Can anyone help me out with a "theoretical" reason? A good hand-waving argument would be fine, unless you have something more official. Thanks in advance, Ken From owner-biophysics@net.bio.net Fri Oct 06 23:00:00 1995 Newsgroups: ott.jobs,ut.physics.general,bionet.biophysics Path: biosci!daresbury!nntp-trd.UNINETT.no!news10.sunet.se!news00.sunet.se!sunic!mn6.swip.net!plug.news.pipex.net!pipex!dish.news.pipex.net!pipex!lade.news.pipex.net!pipex!tank.news.pipex.net!pipex!newsfeed.internetmci.com!hookup!cunews!freenet.carleton.ca!FreeNet.Carleton.CA!ar838 From: ar838@FreeNet.Carleton.CA (Joel Lecorre) Subject: SOIL PHYSICS: Permanent in Alberta - Fed. gov't. Message-ID: Sender: ar838@freenet3.carleton.ca (Joel Lecorre) Reply-To: ar838@FreeNet.Carleton.CA (Joel Lecorre) Organization: The National Capital FreeNet Date: Sat, 7 Oct 1995 10:21:42 GMT Lines: 67 SOIL PHYSICS & MANAGEMENT TECHNICIAN Permanent position with Agriculture and Agrifood Canada, Northern Agriculture Research Centre, Beaverlodge, Alberta. Salary - $37,535 to $45,665 per annum Post secondary training in the advanced courses in soil physics, soil chemistry and statistics from an accredited university or equivalent institution; or an acceptable combination of education, training and experience. Experience in soil and plant analysis using a variety of instrumental methods. In conducting soil management research experiments and operation of field equipment for measuring soil physical properties. In data analysis and plotting using a computer. Proficiency in the use of the English language is essential Fax your resume to the Public Service Commission at (403) 495-2098. Please quote job title and reference number S-95-61-R-36474 on your application. Deadline: October 20, 1995. Note; We thank all those who apply and advise that only those chosen for further consideration will be contacted. In accordance with the Public Service Employment Act, preference will be given to Canadian citizens. We are committed to employment equity. ---------- TECHNICIEN OU TECHNICIENNE DE LA PHYSIQUE ET DE LA GESTION DES SOLS Poste permanent a Agriculture et Agro-alimentaire Canada, Centre de recherche agricole du Nord, Beaverlodge (Alberta). Salaire: 37 535 $ - 45 665 $ par annee Etudes postsecondaires avec cours avances en physique des sols, chimie des sols et statistique suivis dans une universite reconnue ou equivalence ou agencement acceptable d'etudes,de formation et d'experience. Experience de l'analyse des sols et des plantes au moyen de diverses methodes instrumentales. Experience de la conduite d'essais et de recherches sur la gestion des sols ainsi que de l'utilisation d'appareils servant a mesurer les proprietes physiques des sols. Experience de l'analyse de donnees et de l'execution de traces sur ordinateur. La connaissance de l'anglais est essentielle. Faites parvenir votre curriculum vitae par telecopieur a la Commission de la fonction publique au (403) 495-2098. Veuillez mentionner le titre du poste et le numero de reference S-95-61-R-36474 dans votre demande. Date limite: 20 octobre 1995. Nota - Nous remercions tous ceux et celles qui soumettent leur candidature; nous ne communiquerons qu'avec les personnes choisies pour la prochaine etape. En raison de la Loi sur l'emploi dans la fonction publique, la preference sera accordee aux citoyens canadiens. Nous souscrivons aux principes d'equite en matiere d'emploi. From owner-biophysics@net.bio.net Sat Oct 07 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: SZABOR@fns.uniba.sk Newsgroups: bionet.biophysics Subject: Help needed Date: 8 Oct 1995 16:46:21 +0100 Lines: 5 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <458rod$rqm@mserv1.dl.ac.uk> X-Char-Esc: 29 X-Charset: ASCII Original-To: biophys@dl.ac.uk X-Pmrqc: 1 Dear netters, I am in a need of the e-mail address of prof.Skulachev. Thanks in advance Roman Szabo szabor@fns.uniba.sk From owner-biophysics@net.bio.net Sun Oct 08 23:00:00 1995 Path: biosci!UCS.ORST.EDU!taoj From: taoj@UCS.ORST.EDU (Ju-Zhou Tao) Newsgroups: bionet.biophysics Subject: thermal sensation and thermoreceptors Date: 8 Oct 1995 17:10:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510090010.RAA08571@gaia.ucs.orst.edu> NNTP-Posting-Host: net.bio.net To all who can help: I am a new international graduate student in physical chemistry. In erlier times of this year when I worked on my thesis for B.Sc in China, I got some strong interests in thermal sensation and thermoreceptors. I would appreciate anyone who would kindly offer me some information on recent developement of this area and recommend me a reading list. Thanks a lot and in advance. From owner-biophysics@net.bio.net Sun Oct 08 23:00:00 1995 Path: biosci!ihnp4.ucsd.edu!swrinde!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!btnet!dispatch.news.demon.net!demon!mail2news.demon.co.uk!sawdust.cvfn.org From: Robert McElwaine Newsgroups: bionet.biophysics Subject: ZERO/REDUCED-Gravity Chamber Date: Mon, 9 Oct 1995 00:57:12 +0100 Lines: 90 Message-ID: X-NNTP-Posting-Host: sawdust.cvfn.org MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII The ZERO/REDUCED-Gravity Chamber described below has obvious potential applications for Chemistry, Biology, Biophysics, Biochemistry, Medical Research, etc., allowing experiments which now can be done ONLY on the Space Shuttle, AT GREAT EXPENSE! Gravity-NEUTRALIZING Air/Spacecraft or ZERO/REDUCED-Gravity Chamber NASA should build an experimental spacecraft based on U.S. Patent #3,626,605, titled "METHOD AND APPARATUS FOR GENERATING A SECONDARY GRAVITATIONAL FORCE FIELD", awarded to Inventor Henry W. Wallace on Dec. 14, 1971. [$3.00 per complete copy from U.S. Patent Office, 2021 Jefferson Davis Hwy., Arlington, VA 22202; correct 7-digit patent number required. Or try getting it via your local public or university library's inter-library loan dept..] In the patent, Figs. 7A and 7B are basically side views of a gravity-NEUTRALIZING FLYING SAUCER, or, if anchored to the ground, a ZERO-GRAVITY CHAMBER [which could have MANY possible GROUND-level applications for science, medicine, manufacturing, etc.]. Each oval diagram shows a motor spinning a central disc at a very high speed, about 28,000 RPM, and also rotating two other discs sandwiched around the first disc, via gears, at a much slower speed, perhaps 2,800 RPM, in the opposite direction. The two outer discs have extensions [counter-balanced via off-center axis] that, as they rotate, alternately make contact with two wide extensions from opposite walls of the spacecraft. The central disc should have shallow spiral-shaped grooves on both sides for air-bearings, to allow the needed very close contact with the two outer discs. I should clarify that each of the two outer discs has ONLY ONE [counter-balanced] extension, each one pointed opposite (180 degrees) the extension of the other disc. VERY CLOSE CONTACT must be made as the disc extensions slide past the wall extensions in order to conduct the "Kinemassic" Energy (term coined by the Inventor) from the discs to the walls in an ALTERNATING CIRCULATION. The most important factor making it work is that the discs, extensions, and outer walls of the spacecraft MUST be made of any material(s) in which a very large majority of the atoms are of isotopes having "HALF-INTEGRAL ATOMIC SPINS", such as copper (3/2). All other parts, etc., should have a minority of such atoms. [See the appropriate column of the table of isotopes in the latest edition of "The Handbook of Chemistry and Physics."] Experimenters should use one motor to spin the center disc [Start small, such as only one or two feet in diameter.], and a 2ND SEPARATE motor to rotate the two outer discs, so their relative speeds can be varied to establish the needed conditions for PROPULSION of the spacecraft via "NEGATIVE WEIGHT" (with the spacecraft's "Kinemassic" field seemingly PUSHING AGAINST the earth's gravitational field, etc.). If we have to put up a space station, establish Moon bases, go to Mars, rendezvous with comets, etc., WHY DO IT THE HARD WAY?! Your favorite university or research company could make a BIG NAME for itself by making a small model of this work. For more information, answers to your questions, etc., please consult my CITED SOURCES. UN-altered REPRODUCTION and DISSEMINATION of this IMPORTANT Information is ENCOURAGED, especially to COMPUTER BULLETIN BOARDS. Robert E. McElwaine B.S., Physics and Astronomy, UW-EC From owner-biophysics@net.bio.net Mon Oct 09 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!elvis.delphi.com!uunet!in1.uu.net!News.MO.NET!usenet From: jholstein@mail.mo.net (Jim Holstein) Newsgroups: bionet.biophysics Subject: Questions on growing crystals w/ induced ultrasound vibrations Date: 10 Oct 1995 02:21:40 GMT Organization: MBHall, Inc Lines: 31 Message-ID: <45clbk$l8s@Twain.MO.NET> NNTP-Posting-Host: jholstein.mo.net X-Newsreader: WinVN 0.92.6 Thank you for reading and responding I am high school senior going considering doing a science fair project on growing crystals under varying effects of ultrasound frequency and am looking for chemists & physicists who would give me your thoughts? statement of the nul-hypothesis: crystal growth rate [mass/10-days] will not be affected by induced ultrasound crystal growth purity [specific gravity ???] will not be ...etc... constants in the experiment: type of crystal = would alum give quick growth and allow purity meaures variables to be controlled: solution concentration: make-up large vat of liquid & use only that keep sealed to avoid evaporation temperature: grow crystals in an unused kiln with door closed. ultrasound: frequency = thought I'd vary +20%, 0, -20% from size of alum molecule amplitude = thought I'd keep constant question: what wattage (amplitude) to use Please CHAT with me on your suggestions of things I should consider when doing this experiment. I love the sciences and am applying to several colleges in bio+chem with a MS in biochem or PhD as an objective. Thank you for helping Matt Holstein, St.Louis, Missouri, USA using my father's internet jholstein@mail.mo.net From owner-biophysics@net.bio.net Mon Oct 09 23:00:00 1995 Path: biosci!chem.rug.nl!fraaije From: fraaije@chem.rug.nl (Hans Fraaije) Newsgroups: bionet.biophysics Subject: stop the idiotes Date: 10 Oct 1995 09:35:34 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: <9510101617.AA13749@rugmd9.chem.rug.nl> NNTP-Posting-Host: net.bio.net I have seen mail advertiseing for: 1) students reports 2) recipes 2) even a crazy chain letter for people with IQ < 10 Is this biophysics??? Could someone please filter this newsgroup. Otherwise I will get out. J.G.E.M.(Hans) Fraaije Internet: fraaije@chem.rug.nl BIOSON Research Institute Groningen Biomolecular Sciences and Biotechnology Institute Dept. Biophysical Chemistry University of Groningen Nijenborgh 4 9747 AG Groningen The Netherlands tel: +31.50.634320 (direct) +31.50.634378 (secr.) fax: +31.50.634800 From owner-biophysics@net.bio.net Mon Oct 09 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!daresbury!not-for-mail From: SZABOR@fns.uniba.sk Newsgroups: bionet.biophysics Subject: evolution of the bioenerg. systems Date: 10 Oct 1995 11:36:48 +0100 Lines: 11 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <45dic0$15n@mserv1.dl.ac.uk> X-Char-Esc: 29 X-Charset: ASCII Original-To: biophys@dl.ac.uk X-Pmrqc: 1 Dear coleagues, I am supposed to do a seminar concerning the evolution of bioenergetic systemes. I have almost no recent references about this topic. I am particularly interested in a problem WHY certain molecules were chosen in the evolution to serve as a key molecules in the bioenergetic processes (i.e. ATP, phosphates in general, O2 etc.). Any information about the current hypotheses or a bionet discussion would help me. Thank you in advance Roman Szabo szabor@fns.uniba.sk From owner-biophysics@net.bio.net Mon Oct 09 23:00:00 1995 Path: biosci!ORACULO.LCC.UFMG.BR!RICO From: RICO@ORACULO.LCC.UFMG.BR Newsgroups: bionet.biophysics Subject: Neurex Date: 10 Oct 1995 13:03:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510102002.NAA10326@net.bio.net> NNTP-Posting-Host: net.bio.net Does anyone would know the FAX number of the NEUREX Corporation? I will rea lly appreciate it. Thanks. R.M. Leao - rmleao@sagui.icb.ufmg.br From owner-biophysics@net.bio.net Tue Oct 10 23:00:00 1995 Path: biosci!ONREUR.NAVY.MIL!ivodyanoy From: ivodyanoy@ONREUR.NAVY.MIL (Igor Vodyanoy) Newsgroups: bionet.biophysics Subject: net abuse Date: 11 Oct 1995 01:53:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <1995Oct11.094500.1027.27689@smtp-gw.ehis.navy.mil> NNTP-Posting-Host: net.bio.net It looks like we have some experienced crook invading our biophysics net. She/he is trying to establish typical schemes for extracting money from idiots. Her/his e-mail address works only one way and addresses for "sale" alternate. I ask our net supervisor to report this to the Postmaster General. From owner-biophysics@net.bio.net Tue Oct 10 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!howland.reston.ans.net!news.cac.psu.edu!news.math.psu.edu!ra.nrl.navy.mil!usenet From: rwadkins@cbmse.nrl.navy.mil (rmw) Newsgroups: bionet.biophysics Subject: Re: stop the idiotes Date: 11 Oct 1995 21:54:03 GMT Organization: His Own Little World Lines: 21 Sender: -Not-Authenticated-[3942] Distribution: world Message-ID: <45hedr$rnk@ra.nrl.navy.mil> References: <9510101617.AA13749@rugmd9.chem.rug.nl> NNTP-Posting-Host: rmw-6900.nrl.navy.mil X-Posted-From: InterNews 1.0.1@rmw-6900.nrl.navy.mil Xdisclaimer: No attempt was made to authenticate the sender's name. In article <9510101617.AA13749@rugmd9.chem.rug.nl> fraaije@chem.rug.nl (Hans Fraaije) writes: > I have seen mail advertiseing for: > 1) students reports > 2) recipes > 2) even a crazy chain letter for people with IQ < 10 > > > Is this biophysics??? > > Could someone please filter this newsgroup. Otherwise I will get out. Hans: This is not biophysics. This is spam. It came from the University of Illinois-Urbana. When you see these posts, please copy them and send your complaints to postmaster@univ_x.edu, where the univ_x.edu is the offending institution. There is no one to filter this group, like so much of usenet. Your complaints will help get this moron's computer account closed. --Randy From owner-biophysics@net.bio.net Tue Oct 10 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!vixen.cso.uiuc.edu!newsrelay.iastate.edu!news.iastate.edu!baker From: baker@iastate.edu (Wayne R. Baker) Newsgroups: bionet.biophysics,bionet.structural-nmr,sci.techniques.mag-resonance Subject: Stop The Whining (was Re: stop the idiotes) Date: 11 Oct 1995 19:29:34 GMT Organization: Biochemistry & Biophysics, Iowa State University Lines: 40 Message-ID: <45h5uu$s8j@news.iastate.edu> References: <9510101617.AA13749@rugmd9.chem.rug.nl> NNTP-Posting-Host: pv0a0b.vincent.iastate.edu Xref: biosci bionet.biophysics:1314 bionet.structural-nmr:809 sci.techniques.mag-resonance:1241 In bionet.biophysics and other groups, things similar to this have been said: :I have seen mail advertiseing for: :1) students reports :2) recipes :2) even a crazy chain letter for people with IQ < 10 : : :Is this biophysics??? : :Could someone please filter this newsgroup. Otherwise I will get out. While I don't like seeing specious posts in the newsgroups I read, the inevitable posts from people whining about how offended or outraged they are is even more annoying. In complaining about the dismal signal-to-noise levels, your post does nothing but reduce it even further. If a "filter" were installed, response times would be much slower and would require someone to invest a non-trivial amount of time and effort into moderating. Are you willing to volunteer? Garbage posts will always be a fact of life on the .net. The nature of the .net is such that the free and rapid exchange of information will invariably be accompanied by a certain level of dimwitted messages. You can either have a fecal hemorrhage over such off-topic posts or you can just chalk it up as part of the price we pay for this quite remarkable communication system. You can also forward a copy of the offending message to news, postmaster or root at the site of origin. This should also provide an incentive, for those who can, to move to newsreaders rather than mailing lists. You can construct killfiles to weed out garbage or just skip articles and not have things clogging up your mailbox. If you don't have access to a newsserver, then it is a personal decision as to whether you think it is worth it to continue reading a group. But make that decision in private so you don't compound the problem for everyone else. Wayne Baker (baker@iastate.edu) He who has a why to live for 4288 Molecular Biology Building can bear almost any how. Iowa State University --Nietzsche Ames IA 50011 (515) 294 0781 From owner-biophysics@net.bio.net Tue Oct 10 23:00:00 1995 Newsgroups: bionet.biophysics Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!btnet!news.compulink.co.uk!cix.compulink.co.uk!usenet From: jsands@cix.compulink.co.uk ("John Sands Bowker S") Subject: EUROPEAN RESEARCH AND DEVELOPMENT DATABASE Message-ID: Organization: Reed Reference Publishing Date: Wed, 11 Oct 1995 09:24:33 GMT X-News-Software: Ameol Lines: 51 I hope nobody minds - I've taken the liberty of crossposting this from sci.research and would like readers here to tell me whether there is a newsgroup even more appropriate for this kind of announcement of a unique contacts database. With data on activities in both commercial and academic sectors, the European R & D Plus CD-ROM is a contact database for research and development. All science and technology disciplines are covered - from aerospace to zoology - to provide unique and unprecedented interdisciplinary access to information on activities across 34 countries of Europe. European R & D Plus integrates and combines the printed Directory of European Research and Development and Who's Who in European Research and Development, covering: - Organisations: around 20,000 entries including more than 10,000 companies and over 5,000 research institutes as well as tertiary education institutes and consultancies. - Individuals: some 100,000 listed - from Research Directors and Project Leaders to Post-doctoral Researchers on fully-funded projects. - Research activities: from privately funded commercial and industrial project development and innovative research to publicly funded (including national government and EC) applied and theoretical academic research projects of a least one year's duration. 18 search criteria are available on the CD-ROM: name; keyword; research subject; organisation type; current research activities; town; country; annual R&D expenditure; number of R&D staff; personnel; contact details; primary position; career; education; publications; nationality; languages spoken; professional memberships. "The publication is undoubtedly a useful starting point for compiling lists of potential sales contacts or identifying possible collaborative research partners." Review in September's Analytical Instrument Industry Report "These new listings published by Bowker-Saur... represent an altogether new level of information about who researches on what, and where, in 34 European countries." Review in September's European Review For more information including enquiries about being included in the database: http://www.bowker-saur.co.uk/service/cdrom/newcdtitles.html or email randd@bowker-saur.co.uk From owner-biophysics@net.bio.net Wed Oct 11 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!elvis.delphi.com!uunet!in2.uu.net!news.biu.ac.il!news.huji.ac.il!MARDER Newsgroups: bionet.biophysics Subject: Re: evolution of the bioenerg. systems Message-ID: <45ig56$fbc_001@agri.huji.ac.il> From: MARDER@agri.huji.ac.il (Jonathan B. Marder) Date: Thu, 12 Oct 95 07:29:42 GMT References: <45dic0$15n@mserv1.dl.ac.uk> Distribution: world Organization: Hebrew University Nntp-Posting-Host: marder.agri.huji.ac.il X-Newsreader: News Xpress Version 1.0 Beta #4 Lines: 35 In article <45dic0$15n@mserv1.dl.ac.uk>, SZABOR@fns.uniba.sk wrote: >Dear coleagues, >I am supposed to do a seminar concerning the evolution of >bioenergetic systemes. I have almost no recent references about this >topic. I am particularly interested in a problem WHY certain >molecules were chosen in the evolution to serve as a key molecules in >the bioenergetic processes (i.e. ATP, phosphates in general, O2 >etc.). Any information about the current hypotheses or a bionet >discussion would help me. Thank you in advance > Roman Szabo > szabor@fns.uniba.sk Have a look at:- Harold, Franklin M. The vital force: a study of bioenergetics New York : W. H. Freeman and Co., c1986. It is not completely up to date, but his approach may be exactly what you are looking for. I know of important updates are in the following areas:- 1. Proton ATPase - similarities and sequence homologies between eukarotic, archebacterial, eubacterial (inc. mitocochondria and plastids) enzymes. See papers/reviews by N. Nelson and others. 2. Photosynthetic Reaction centres - the connection between bacterial and chloroplast reaction centres (reviews by Michel, Trebst, Barber etc.). I hope that this helps. Jonathan B. Marder ' Department of Agricultural Botany | Internet: MARDER@agri.huji.ac.il The Hebrew University of Jerusalem | /\/ Faculty of Agriculture |/ \ Phone: (08 or +9728) 481918 P.O.Box 12, Rehovot 76100, ISRAEL / Fax: (08 or +9728) 467763 From owner-biophysics@net.bio.net Wed Oct 11 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!warwick!yama.mcc.ac.uk!io.salford.ac.uk!aber!innews From: prm@aber.ac.uk (Pedro Mendes) Newsgroups: bionet.biophysics,bionet.metabolic-reg Subject: Re: evolution of the bioenerg. systems Date: 12 Oct 1995 11:51:45 GMT Organization: University of Wales Aberystwyth Lines: 27 Distribution: bionet Message-ID: <45ivgh$gnl@fileserv.aber.ac.uk> References: <45dic0$15n@mserv1.dl.ac.uk> NNTP-Posting-Host: pcfchb.dbs.aber.ac.uk X-Disclaimer: Warning - Sender not verifiable X-Newsreader: WinVN 0.99.6 NT Xref: biosci bionet.biophysics:1319 bionet.metabolic-reg:564 In article <45dic0$15n@mserv1.dl.ac.uk>, SZABOR@fns.uniba.sk says... > >Dear coleagues, >I am supposed to do a seminar concerning the evolution of >bioenergetic systemes. I have almost no recent references about this >topic. I am particularly interested in a problem WHY certain >molecules were chosen in the evolution to serve as a key molecules in >the bioenergetic processes (i.e. ATP, phosphates in general, O2 >etc.). Any information about the current hypotheses or a bionet >discussion would help me. Thank you in advance Take a look at Wachtershauser, G. (1988) Before enzymes and templates - theory of surface metabolism. Microbiol. Rev. 52, 452-484. Where a detailed hypothesis is put forward (not only on bioenergetics but metabolism in general) I have cross-posted this message to bionet.metabolic-reg where this subject is on topic! -- Pedro Mendes Instit. Biological Sciences prm@aber.ac.uk (home: pedro@enzyme.demon.co.uk) Univ. Wales, Aberystwyth, http://gepasi.dbs.aber.ac.uk/pedro/prmhome.htm Dyfed SY23 3DA, U.K. PGP mail welcome! (public key on demand) FAX: +44 (0)1970 622350 From owner-biophysics@net.bio.net Thu Oct 12 23:00:00 1995 Path: biosci!LEX.LCCC.EDU!rcb1 From: rcb1@LEX.LCCC.EDU (Ron Blue) Newsgroups: bionet.biophysics Subject: Re: evolution of the bioenerg. systems Date: 13 Oct 1995 05:53:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <45ig56$fbc_001@agri.huji.ac.il> > In article <45dic0$15n@mserv1.dl.ac.uk>, SZABOR@fns.uniba.sk wrote: > >Dear coleagues, > >I am supposed to do a seminar concerning the evolution of > >bioenergetic systemes. I have almost no recent references about this > >topic. I am particularly interested in a problem WHY certain > >molecules were chosen in the evolution to serve as a key molecules in > >the bioenergetic processes (i.e. ATP, phosphates in general, O2 > >etc.). Any information about the current hypotheses or a bionet > >discussion would help me. Thank you in advance > > Roman Szabo > > szabor@fns.uniba.sk This may sound like a strange idea. The transport of chemicals in microtubules may be influenced by modulating electrical fields. So a particular oscilation would chose NATURALLY a particular molecular structure. Molecular structures that could be selected using the above procedure could be selected in by evolution. This might explain why slight variation (assuming a gaussian effect) could drift to new functions. This is pure speculation. Ron Blue From owner-biophysics@net.bio.net Thu Oct 12 23:00:00 1995 Path: biosci!newshost.lanl.gov!usenet From: Stefan Burde Newsgroups: bionet.biology.grasses,bionet.biology.n2-fixation,bionet.biology.tropical,bionet.biophysics,bionet.celegans Subject: Re: Make a $1,000,000 in 90 days! Date: 11 Oct 1995 17:37:15 GMT Organization: Los Alamos National Laboratory Lines: 8 Message-ID: <45gvcb$5bg@newshost.lanl.gov> References: <45fr0q$t07$2@mhadf.production.compuserve.com> NNTP-Posting-Host: fish.lanl.gov Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: 102771.3337@CompuServe.COM X-URL: news:45fr0q$t07$2@mhadf.production.compuserve.com Xref: biosci bionet.biology.grasses:398 bionet.biology.n2-fixation:368 bionet.biology.tropical:1354 bionet.biophysics:1322 bionet.celegans:624 GDR Enterprises <102771.3337@CompuServe.COM> wrote: > $$$ Change Your Life $$$ >etc. Please spare us the crap mail From owner-biophysics@net.bio.net Fri Oct 13 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!chi-news.cic.net!simtel!swidir.switch.ch!swsbe6.switch.ch!scsing.switch.ch!news.belwue.de!news.uni-ulm.de!rz.uni-karlsruhe.de!news.uni-stuttgart.de!uni-regensburg.de!faui0n.informatik.uni-erlangen.de!uni-erlangen.de!winx03!wpxx02.toxi.uni-wuerzburg.de!krasel From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.biophysics Subject: Re: stop the idiotes Date: 13 Oct 1995 21:51:45 GMT Organization: Dept. of Pharmacology, U Wuerzburg Lines: 31 Distribution: world Message-ID: <45mn1h$o16@winx03.informatik.uni-wuerzburg.de> References: <9510101617.AA13749@rugmd9.chem.rug.nl> NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [version 1.2 PL2] Hans Fraaije (fraaije@chem.rug.nl) wrote: > I have seen mail advertiseing for: > 1) students reports > 2) recipes > 2) even a crazy chain letter for people with IQ < 10 > Is this biophysics??? No, it is called "Usenet". As long as a newsgroup is unmoderated, everybody can post what they like. > Could someone please filter this newsgroup. Otherwise I will get out. While it is possible to make a newsgroup moderated, there are two caveats: a) you have to find a moderator who is willing to spend his/her time reading postings to the group b) the current moderation scheme can be bypassed quite easily by somebody who is really determined Well, that's the way Usenet works. If you can live with it, make the best of it. If you can't, ... --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de */ /* "Science is the game we play with God to find out what His rules are." */ From owner-biophysics@net.bio.net Fri Oct 13 23:00:00 1995 Path: biosci!neubio.sld.ar!Postmaster From: Postmaster@neubio.sld.ar (Administrador del Nodo) Newsgroups: bionet.biophysics Subject: EMF-modulated transport of chemicals inside microtubules Date: 14 Oct 1995 07:51:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 88 Sender: daemon@net.bio.net Distribution: world Message-ID: <139jn989@neubio.sld.ar> NNTP-Posting-Host: net.bio.net Hello, netters! Ron Blue is certainly right in saying that evolution could select molecular structures (adaptively) influenced by particular oscilations arising out of EMF-modulation inside biological tissues, as a pro- pelling mean to transport such molecular structures within microtubules (in collaboration with other already known mechanisms for that molecular transport). While such evolutive possibility certainly exists and deserves to be seriously explored, Ron is also correct, upon my knowledge, in saying that this is (still) pure speculation. (If anybody knows ANY observational or experimental validation made independently of the same co- lleagues that forwarded such speculations, please!!! let me know of it). However, there is a tissue where such speculations evolved more luxuriantly than in others. Said tissue is the sentient and thinking condensed matter, to wit the neurocognitive parenchymae in the brain. As many of the netters are aware, to avoid relinquishing the inanity of nature most models of brain psychogenesis contrived recondite sites to reduce sentience to motions and, then, translate these into even more recondite jitterinesses, thence amplifiable so as to gain some observational plausibility; the ungranted step is of course said reduction. But this is not our subject here. Lea- ving it be what it be, the following modelling steps concern with our subject. In this regard, since 1906, in our tradition models were forwarded of the making of wavelet-like entities through interfering reverberances in the brain gray parenchymae. Their originator was Prof. Christfried Jakob (1866-1956). After his death these models were followed, independently, by Prof. M. Cro- cco and disciples here and by Prof. K.H. Pribram and disciples in North America. From this last line of work in the last ten years a sub-line of explanatory models arose, of which I still do not know independent experimental validations, forwarding detailed models of chemical transport and even laser-like reactions inside microtubules through the modulation of background EMF's. From the many papers expounding these models, let me choose for your reading the following: Jibu, M.; Hagan, S., Hameroff, S.R., Pribram, K.H. and Yasue, K., "Quantum optical coherence in cytoskeletal microtubules: impli- cations for brain function", Biosystems (Ireland) 32 (3), pp. 195-209, 1994 (ISSN 0303-2647). While we cannot follow these speculations without having at least the essential steps fixed by means of independent experimental validation (and moreover we cannot share many of the prefigurati- ve tenets included in those models), we are on the other hand sure that the mechanism mentioned by Ron Blue is sound and can be bols- tered in evolutive scenarios. However most of the papers I read concerning it are written by medical practitioners or theoretical biologists without full command of biophysical subtleties. So I think that it might be important to essay a biophysical inter- change of notices and ideas about the details of such mechanisms, and that Ron Blue's mention makes good an occassion to trigger it. Does anybody know more of these models so as to share bibliography and discussing some points in physicochemistry? Cheers, Mariela =*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*= Prof. Mariela Szirko, Centro de Investig. Neurobiologicas, Ministry of Health & Welfare, Argentine Republic; and Lab. of Electroneurobiological Res., Hospital "Dr. Jose Tiburcio Borda", Municipality of Buenos Aires, Office: Phone/Fax (54 1) 306 -7314 e-mail Standard disclaimer: Las opiniones de este mensaje son personales y no comprometen las dependencias a cargo de la firmante. Reply to THIS message, ONLY to: =*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*= From owner-biophysics@net.bio.net Fri Oct 13 23:00:00 1995 Path: biosci!NET.BIO.NET!biosci-help From: biosci-help@NET.BIO.NET (BIOSCI Administrator) Newsgroups: bionet.biophysics Subject: PLEASE READ - IMPORTANT change to BIOPHYSICS mailing list!!! Date: 14 Oct 1995 16:17:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Sender: daemon@net.bio.net Distribution: world Message-ID: Reply-To: biosci-help@net.bio.net NNTP-Posting-Host: net.bio.net We are doing some important clean-up work on our older mailing lists that were established before we adopted the majordomo list management software a few years back. This mailing list is one of the ones affected by this work. The changes do not affect the parallel USENET newsgroup associated with this mailing list. So that the e-mail subscription routines will be consistent for all of the BIOSCI mailing lists at net.bio.net, we are adopting the convention that the <= 8 character e-mail address for each mailing list will be the text that is used in the "subscribe" and "unsubscribe" commands sent to biosci-server@net.bio.net. This message only affects e-mail users who signed up on the mailing lists at the U.S. BIOSCI node at net.bio.net. All others can ignore the remaining details below. Please note that the UK BIOSCI node uses different mailing list management software which incorporates the bionet USENET newsgroup name in the SUB command, e.g., sub bionet-news.bionet.jobs.offered. UK BIOSCI subscribers should ignore the following info. The old server commands for this newsgroup were subscribe biophysics unsubscribe biophysics The NEW server commands are subscribe biophys unsubscribe biophys PLEASE DO NOT send these commands to the mailing list address. You will bother everyone on the list and not get yourself added to the list. All subscribe and unsubscribe commands for the Americas and Pacific Rim country BIOSCI list users should be addressed to biosci-server@net.bio.net. BIOSCI readers in Europe, Africa and Central Asia should use the U.K. BIOSCI node (please contact biosci@daresbury.ac.uk for details). If your address is on the U.S. BIOSCI mailing list for this newsgroup, please save this message for future reference. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-biophysics@net.bio.net Sat Oct 14 23:00:00 1995 Newsgroups: bionet.biophysics Path: biosci!rutgers!uwm.edu!chi-news.cic.net!newsfeed.internetmci.com!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!hgmp.mrc.ac.uk!ebi.ac.uk!EMBL-EBI.ac.uk!tflores From: tflores@EMBL-EBI.ac.uk (Tom Flores) Subject: Re: Janet Thornton's e-mail address? Sender: news@ebi.ac.uk (Mr news) Message-ID: Date: Fri, 13 Oct 1995 17:35:53 GMT Lines: 23 Reply-To: tflores@EMBL-EBI.ac.uk (Tom Flores) References: <45m2so$qo9@decaxp.harvard.edu> Organization: European Bioinformatics Institute (EMBL) - UK X-Newsreader: mxrn 6.18-32 In article <45m2so$qo9@decaxp.harvard.edu>, Zorro writes: > >Hi. Does anyone have an e-mail (or snail mail) address for Professor >Janet M. Thornton, of University College, London? > >Thanks in advance, > >Z. > thornton@bsm.bioc.ucl.ac.uk -- ============================================================ Tomas P. Flores PhD Tel:+44-(0)1223 494414 EMBL Outstation Fax:+44-(0)1223 494468 The European Bioinformatics Institute Hinxton Hall Hinxton Cambridge CB10 1RQ UK Email:Tom.Flores@ebi.ac.uk ============================================================ From owner-biophysics@net.bio.net Sun Oct 15 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.sprintlink.net!dispatch.news.demon.net!demon!tank.news.pipex.net!pipex!warwick!yama.mcc.ac.uk!io.salford.ac.uk!aber!innews From: prm@aber.ac.uk (Pedro Mendes) Newsgroups: bionet.biophysics,bionet.metabolic-reg Subject: Re: evolution of the bioenerg. systems Date: 16 Oct 1995 10:22:22 GMT Organization: University of Wales Aberystwyth Lines: 39 Distribution: world Message-ID: <45tbou$djo@fileserv.aber.ac.uk> References: <45dic0$15n@mserv1.dl.ac.uk> <45ig56$fbc_001@agri.huji.ac.il> NNTP-Posting-Host: pcfchb.dbs.aber.ac.uk X-Disclaimer: Warning - Sender not verifiable X-Newsreader: WinVN 0.99.6 NT Xref: biosci bionet.biophysics:1327 bionet.metabolic-reg:568 NOTE: this has been cross-posted to bionet.metabolic-reg where bioenergetics is a main topic! I am not the original poster of the following text, I have just been asked to relay these contributions by their authors: ---forwarded mail From: Marta Cascante Dear collegue, There is a very interesting paper from prof. V.P. Skulachev entitled bioenergetics: the evolution of molecular mechanisms and the development of bioenergetic concepts. I hope it will be usefull to you. In general Dr. Skulachev papers can interese you because he has done a nice and exciting work in this field. The reference of the article is: Antonie van Leeuwenhoek 65,271-284 Kluvier Academic Publishers. Best wishes Yours sincerely Marta Cascante ---forwarded mail From: michels@trop.ucl.ac.be Are you aware of the book 'Blueprint for a cell; the nature and origin of life' by Christian De Duve (1991, Neil Patterson Publishers, North Carolina ; ISBN 0-89278-410-5) ? Chapter 7 ('Harnessing Energy') may be relevant with regard to your questions. Best wishes, Paul Michels -- Pedro Mendes Inst. Biological Sciences prm@aber.ac.uk (home: pedro@enzyme.demon.co.uk) Univ. Wales, Aberystwyth, http://gepasi.dbs.aber.ac.uk/pedro/prmhome.htm Dyfed SY23 3DA, U.K. PGP mail welcome! (public key on demand) FAX: +44 (0)1970 622350 From owner-biophysics@net.bio.net Mon Oct 16 23:00:00 1995 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.biophysics Subject: IMPORTANT: BIOSCI miniFAQ Date: 17 Oct 1995 02:02:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 196 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510170900.CAA08984@net.bio.net> NNTP-Posting-Host: net.bio.net This is a new "miniFAQ" designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) What to do about "spams," i.e., junk mail, ads, etc. 2) Examples of subscribing and unsubscribing to the mailing lists. 3) How to access BIOSCI/bionet newsgroup archives. 4) The BIOSCI user address and research interest directory. 1) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups) and mailing lists. The same postings are distributed on both media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the newsgroups from about 95% of the spams that are being sent to date. This means that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings. Unfortunately there are easy ways for determined spammers to override the moderation mechanism. We are working on new systems to provide access to our newsgroups over the WWW. These should be available soon, probably November 1995, and will allow you to use your Web browser to look at the news postings. While this will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. 2) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 3) How to access BIOSCI/bionet newsgroup archives. -------------------------------------------------- Back postings of all BIOSCI/bionet newsgroups can be found on the World Wide Web at URL http://www.bio.net/. There are several searchable newsgroup indices at this site. E-mail users can search the BIOSCI archives by using our waismail e-mail server. For instructions send the message help to waismail@net.bio.net. Leave the Subject: line blank (anything entered on the Subject: line is ignored). 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-biophysics@net.bio.net Mon Oct 16 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!usenet.ucs.indiana.edu!bronze.ucs.indiana.edu!jverburg From: jverburg@ucs.indiana.edu (John M. VerBurg) Newsgroups: bionet.biophysics,bionet.structural-nmr,sci.techniques.mag-resonance Subject: Re: Stop The Whining (was Re: stop the idiotes) Followup-To: bionet.biophysics,bionet.structural-nmr,sci.techniques.mag-resonance Date: 17 Oct 1995 17:02:13 GMT Organization: Indiana University Lines: 13 Message-ID: <460nil$mvr@usenet.ucs.indiana.edu> References: <9510101617.AA13749@rugmd9.chem.rug.nl> <45h5uu$s8j@news.iastate.edu> NNTP-Posting-Host: nickel.ucs.indiana.edu X-Newsreader: TIN [version 1.2 PL2] Xref: biosci bionet.biophysics:1329 bionet.structural-nmr:825 sci.techniques.mag-resonance:1248 Wayne R. Baker (baker@iastate.edu) wrote: [snip] : But make that decision in private so you don't compound the problem : for everyone else. I agree completely. I too hate spams, but I believe the best way to protest them is to reply to the original poster and feign interest in their scam and do nothing. They will waste their time following up on a false lead. Imagine if 10,000 people did this. To save time, I usually just write "me too" in the relpy, along with their post. jverburg From owner-biophysics@net.bio.net Tue Oct 17 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!simtel!zombie.ncsc.mil!cs.umd.edu!haven.umd.edu!purdue!oitnews.harvard.edu!fas-news.harvard.edu!fas.harvard.edu!berriz From: Zorro Newsgroups: bionet.molbio.proteins,bionet.biophysics Subject: Protein model kits anywhere? Date: 18 Oct 1995 21:52:50 GMT Organization: Harvard University, Cambridge, Massachusetts Lines: 17 Message-ID: <463svi$7l8@decaxp.harvard.edu> Reply-To: berriz@husc.harvard.edu NNTP-Posting-Host: fas.harvard.edu X-Newsreader: NN version 6.5.0 #3 (NOV) Originator: berriz@fas.harvard.edu Xref: biosci bionet.molbio.proteins:6007 bionet.biophysics:1330 Hello. I'm sure you're all familiar with the standard molecular kits used by organic chemists to build "hand held" (as opposed to "computer") 3D models of molecules. The main reason I don't like using these for modelling proteins is that with them it is hard to prevent rotations around the peptide bonds while performing allowed rotations around other bonds. My question is, do specialized, off-the-shelf kits exist for modelling proteins? (For example, it would be handy if one could get long chunks of ready-made backbone, with all bonds free to rotate, except for the peptide bonds.) If so, who sells them? Thanks in advance for your suggestions. Z. From owner-biophysics@net.bio.net Fri Oct 20 23:00:00 1995 Path: biosci!agate!spool.mu.edu!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!lll-winken.llnl.gov!decwrl!pagesat.net!netserv.com!aimnet.com!news2.aimnet.com!usenet From: xyzzyx@aimnet.com (xyzzyx) Newsgroups: bionet.molbio.proteins,bionet.biophysics Subject: Re: Protein model kits anywhere? Date: 21 Oct 1995 04:50:36 GMT Organization: xyzzyx consultants Lines: 31 Message-ID: <469u6s$bgf@news2.aimnet.com> References: <463svi$7l8@decaxp.harvard.edu> NNTP-Posting-Host: 204.118.160.36 Mime-Version: 1.0 Content-Type: Text/Plain; charset=ISO-8859-1 X-Newsreader: WinVN 0.99.5 Xref: biosci bionet.molbio.proteins:6057 bionet.biophysics:1338 In article <463svi$7l8@decaxp.harvard.edu>, berriz@husc.harvard.edu says... > > > >Hello. I'm sure you're all familiar with the standard molecular kits >used by organic chemists to build "hand held" (as opposed to >"computer") 3D models of molecules. The main reason I don't like >using these for modelling proteins is that with them it is hard to >prevent rotations around the peptide bonds while performing allowed >rotations around other bonds. > >My question is, do specialized, off-the-shelf kits exist for modelling >proteins? (For example, it would be handy if one could get long >chunks of ready-made backbone, with all bonds free to rotate, except >for the peptide bonds.) If so, who sells them? > >Thanks in advance for your suggestions. > >Z. Sorry, never seen one, but it is a great idea. A while back I saw an ad for a machine that built up three dimensional models from space coordinates by polymerizing a monomer solution with a laser. I honestly can't remember who made it, but that might be a really nice way to construct physically accurate amino acids and nucleotides. Once you had a prototype, you could probaly mass produce them pretty easily. I'm sure they'd be a great seller for grad students and academics. Good luck in your search. From owner-biophysics@net.bio.net Fri Oct 20 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!news.sprintlink.net!news.interserv.net!usenet From: 100145.3141@compuserve.com Newsgroups: bionet.biophysics Subject: Test, don't read Date: 21 Oct 1995 08:56:17 GMT Organization: InterServ News Service Lines: 2 Message-ID: <46acjh$pjr@data.interserv.net> NNTP-Posting-Host: dd44-141.compuserve.com X-Newsreader: AIR News 3.X (SPRY, Inc.) Test, don't read From owner-biophysics@net.bio.net Sat Oct 21 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!lll-winken.llnl.gov!enews.sgi.com!sgigate.sgi.com!sgiblab!cgl!friedman From: friedman@cgl.ucsf.edu (Simon Friedman) Newsgroups: bionet.molbio.proteins,bionet.biophysics Subject: Re: Protein model kits anywhere? Date: 22 Oct 1995 04:58:27 GMT Organization: Computer Graphics Laboratory, UCSF Lines: 41 Message-ID: <46cj1j$ujo@cgl.ucsf.edu> References: <463svi$7l8@decaxp.harvard.edu> NNTP-Posting-Host: socrates.ucsf.edu Xref: biosci bionet.molbio.proteins:6061 bionet.biophysics:1339 In article <463svi$7l8@decaxp.harvard.edu> berriz@husc.harvard.edu writes: > > >Hello. I'm sure you're all familiar with the standard molecular kits >used by organic chemists to build "hand held" (as opposed to >"computer") 3D models of molecules. The main reason I don't like >using these for modelling proteins is that with them it is hard to >prevent rotations around the peptide bonds while performing allowed >rotations around other bonds. > >My question is, do specialized, off-the-shelf kits exist for modelling >proteins? (For example, it would be handy if one could get long >chunks of ready-made backbone, with all bonds free to rotate, except >for the peptide bonds.) If so, who sells them? > >Thanks in advance for your suggestions. > >Z. Every modeling kit I have seen allows you to model double bonds or bonds with double bond character and hindered rotation. In one system this is accomplished by having two sticks in parallel form the bond. So, in the peptide bond, the amide N and carbonyl C have a stick between them, and a second stick that snaps on to the two atoms on the side. This allows only small deviations from planarity for the bond, which is a fair representation of the peptide bond. So check out the kit at your bookstore closely. I'm guessing it has some sort of provision for modeling double bonds/partial double bonds. If no, I can post the name of my kit (which I can't remember off hand). Simon From owner-biophysics@net.bio.net Sat Oct 21 23:00:00 1995 Newsgroups: bionet.molbio.proteins,bionet.biophysics Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!delmarva.com!udel!darwin.sura.net!nih-csl!rvenable From: rvenable@deimos.cber.nih.gov (Rick Venable) Subject: Re: Protein model kits anywhere? Message-ID: <1995Oct22.155947.20941@alw.nih.gov> Followup-To: bionet.molbio.proteins,bionet.biophysics Sender: postman@alw.nih.gov (AMDS Postmaster) Nntp-Posting-Host: deimos.cber.nih.gov Organization: FDA/CBER Biophysics Lab X-Newsreader: TIN [version 1.2 PL2] References: <463svi$7l8@decaxp.harvard.edu> Date: Sun, 22 Oct 1995 15:59:47 GMT Lines: 26 Xref: biosci bionet.molbio.proteins:6065 bionet.biophysics:1340 On 18 Oct 1995 21:52:50 GMT Zorro asked: > Hello. I'm sure you're all familiar with the standard molecular kits > used by organic chemists to build "hand held" (as opposed to > "computer") 3D models of molecules. The main reason I don't like > using these for modelling proteins is that with them it is hard to > prevent rotations around the peptide bonds while performing allowed > rotations around other bonds. > My question is, do specialized, off-the-shelf kits exist for modelling > proteins? (For example, it would be handy if one could get long > chunks of ready-made backbone, with all bonds free to rotate, except > for the peptide bonds.) If so, who sells them? You may wish to look around for Maruzen models, which I think are from Japan. I believe you can order amino and nucleic acids pre-assembled from component atoms. I've seen them in a colleague's laboratory, so I know they exist, but I can't say for certain if they meet your extra requirement concerning peptide bonds. That bond only has partial double bond character, and the barrier to rotation is only about 10%-15% that of a true double bond (but several times that of a single bond). You can see that distinction may be a problem, when balancing accuracy vs. convenience. -- Rick Venable =====\ |=| "Eschew Obfuscation" FDA/CBER Biophysics Lab |____/ |=| Bethesda, MD U.S.A. | \ / |=| / Not an official statement \ rvenable@deimos.cber.nih.gov \/ |=| \ or position of the FDA. / From owner-biophysics@net.bio.net Sun Oct 22 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.sprintlink.net!simtel!zombie.ncsc.mil!cs.umd.edu!haven.umd.edu!purdue!lerc.nasa.gov!magnus.acs.ohio-state.edu!csn!carbon!hermes.cair.du.edu!usenet From: dbovee@phoebe.cair.du.edu (David Bovee) Newsgroups: bionet.molbio.proteins,bionet.biophysics Subject: Re: Protein model kits anywhere? Date: 23 Oct 1995 02:04:44 GMT Organization: University of Denver Lines: 31 Message-ID: <46et7s$c5@hermes.cair.du.edu> References: <463svi$7l8@decaxp.harvard.edu> <46cj1j$ujo@cgl.ucsf.edu> NNTP-Posting-Host: sl-23.ducomm.du.edu Mime-Version: 1.0 Content-Type: Text/Plain; charset=ISO-8859-1 X-Newsreader: WinVN 0.99.6 Xref: biosci bionet.molbio.proteins:6066 bionet.biophysics:1342 In article <46cj1j$ujo@cgl.ucsf.edu>, friedman@cgl.ucsf.eduÍ says... > >In article <463svi$7l8@decaxp.harvard.edu> berriz@husc.harvard.edu writes: >> >>...My question is, do specialized, off-the-shelf kits exist for modelling >>proteins? (For example, it would be handy if one could get long >>chunks of ready-made backbone, with all bonds free to rotate, except >>for the peptide bonds.) If so, who sells them? >> > >Every modeling kit I have seen allows you to model double bonds >or bonds with double bond character and hindered rotation. In one system >this is accomplished by having two sticks in parallel form the bond. >So, in the peptide bond, the amide N and carbonyl C have a stick >between them, and a second stick that snaps on to the two >atoms on the side. This allows only small deviations from >planarity for the bond, which is a fair representation of the >peptide bond. >So check out the kit at your bookstore closely. I'm guessing it has >some sort of provision for modeling double bonds/partial double bonds. >If no, I can post the name of my kit (which I can't remember off hand). > >Simon > Generally, this type of physical modeling kit does not allow for such bonds without using up one of the available bonding sites. For example, modeling a peptide backbone using a double bond C=N and C=O (which is pretty close to reality) would use up all four of the bonding sites on the C atom. The only way that type of kit would be modifiable for the type of backbone which "Z" wants is if the atoms had sites for more than then are neutrally allowed. From owner-biophysics@net.bio.net Sun Oct 22 22:00:00 1995 Path: biosci!CS.Arizona.EDU!news.Arizona.EDU!hamblin.math.byu.edu!sol.ctr.columbia.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!tank.news.pipex.net!pipex!oleane!jussieu.fr!nef.ens.fr!news From: CHAIEB Sahraoui Newsgroups: bionet.biophysics Subject: Enzyme Date: 21 Oct 1995 14:57:21 GMT Organization: Ecole Normale Superieure Lines: 7 Message-ID: <46b1oh$o83@nef.ens.fr> NNTP-Posting-Host: falbala.ens.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; HP-UX A.09.05 9000/712) X-URL: news:bionet.biophysics Can anybody tell me , if an enzyme able to cat the tails of a phospholipid exists, Or one of the tails of the phospholipid, is also interesting. please reply to my personnal e-mail adress, I'm not subscribed to this newsgroup Thanks my email: chaieb@physique.ens.fr From owner-biophysics@net.bio.net Sun Oct 22 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!uunet!in2.uu.net!newsflash.concordia.ca!news.mcgill.ca!ewbank From: ewbank@monod.biol.mcgill.ca (Jonathan Ewbank) Newsgroups: bionet.molbio.proteins,bionet.biophysics Subject: Re: Protein model kits anywhere? Date: 23 Oct 1995 23:08:26 GMT Organization: McGill University Computing Centre Lines: 23 Message-ID: <46h79a$ggc@sifon.cc.mcgill.ca> References: <463svi$7l8@decaxp.harvard.edu> <46cj1j$ujo@cgl.ucsf.edu> <46et7s$c5@hermes.cair.du.edu> NNTP-Posting-Host: monod.biol.mcgill.ca Xref: biosci bionet.molbio.proteins:6076 bionet.biophysics:1348 >>> >>>...My question is, do specialized, off-the-shelf kits exist for modelling >>>proteins? (For example, it would be handy if one could get long >>>chunks of ready-made backbone, with all bonds free to rotate, except >>>for the peptide bonds.) If so, who sells them? >>> Nicholson model components from: Labquip Ashridgewood Place, Forest Road, Wokingham, Reading RG11 5RA ENGLAND (info from Tom Creighton's "Proteins" [2nd edition, 1993]) i've used them to build a small protein (120 a.a); they work well. j. ewbank@monod.biol.mcgill.ca From owner-biophysics@net.bio.net Sun Oct 22 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!howland.reston.ans.net!vixen.cso.uiuc.edu!usenet From: Fred Barthel Newsgroups: bionet.general,bionet.biophysics,bionet.cellbiol,bionet.microbiology Subject: Input from Scientists needed Date: 23 Oct 1995 15:24:24 GMT Organization: University of Illinois at Urbana Lines: 32 Message-ID: <46gc38$okv@vixen.cso.uiuc.edu> NNTP-Posting-Host: seating.rehab.uiuc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Xref: biosci bionet.general:18056 bionet.biophysics:1346 bionet.cellbiol:3240 bionet.microbiology:3710 WHY IS IT SO DIFFICULT FOR PEOPLE WITH DISABILITIES TO ENTER AND SUCCEED IN CAREERS IN SCIENCE, ENGINEERING, & MATHEMATICS? Project PURSUIT needs your insights! We are seeking input from professionals in the fields of science, engineering, and mathematics, educators, students, parents, rehabilitation professionals, and anyone else who would like to contribute. PURSUIT, an National Science Foundation-funded project, is researching the underrepresentation of people with disabilities in the careers of science, engineering, and mathematics. People with disabilities encounter numerous barriers which may hinder them in the classroom, in laboratories, and in the workplace. Whether or not you have experience with people with disabilities, we would greatly value your ideas on this subject. To complete the survey we've created on the topic, please go to the following WWW URL: http://pursuit.rehab.uiuc.edu/pursuit/t-survey.html Or write to us for a print or electronic copy at PURSUIT@uiuc.edu For more information on Project PURSUIT and our activities, feel free to contact us at: Project PURSUIT 1207 South Oak Street Champaign, IL 61820 (800) 367-1736 PURSUIT@uiuc.edu Our WWW home page: http://pursuit.rehab.uiuc.edu From owner-biophysics@net.bio.net Sun Oct 22 22:00:00 1995 Newsgroups: bionet.biophysics Path: biosci!ihnp4.ucsd.edu!swrinde!tank.news.pipex.net!pipex!newsfeed.internetmci.com!news.sprintlink.net!in1.uu.net!hearst.acc.Virginia.EDU!murdoch!bootp-18-241.bootp.virginia.edu!user From: saj3y@mcconnell.chem.virginia.edu (sajith jayasinghe) Subject: help preperation of GUVs X-Nntp-Posting-Host: bootp-18-241.bootp.virginia.edu Message-ID: Sender: usenet@murdoch.acc.Virginia.EDU Organization: department of chemistry Date: Mon, 23 Oct 1995 14:29:14 GMT Lines: 11 Hi, i was wondering if anybody out there had any experinece in making GUVs (giant unilamellar vesicels). I have been following the procedure we found in an article (biochem 32, 9936-9943, 1993) using dialysis. The GUVs wer made with a lipid conc of 20mg/ml or so. I need to increase the lipid conc to about 80 mg/ml but am worried about vesicle aggregation or other effects (like flattened vesicles). Any ideas? any suggestions appreciated thanks sajith jayasinghe e-mail: saj3y@mcconnell.chem.virginia.edu From owner-biophysics@net.bio.net Tue Oct 24 22:00:00 1995 Path: biosci!medinst.doe.ernet.in!dinesh From: dinesh@medinst.doe.ernet.in Newsgroups: bionet.biophysics Subject: Notice for Conference Date: 24 Oct 1995 09:00:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 87 Sender: daemon@net.bio.net Distribution: world Message-ID: <9510241622.AA18309@doe.ernet.in> NATIONAL SYMPOSIUM ON MOLECULAR AND CELLULAR BIOPHYSICS and 23rd annual meeting of INDIAN BIOPHYSICAL SOCIETY (IBS) FEBRUARY 18-21, 1996, NEW DELHI, INDIA Convener : Dr. V. Kothekar, Add'l. Prof., Department of Biophysics, All India Institute of Medical Sciences, India. Phone : 661123 Extn. 3215 E-mail : kothekar@medinst.ernet.in ************************************************************ INVITATION AND CALL FOR PAPERS ORGANISED BY ALL INDIA INSTITUTE OF MEDICAL SCIENCES, INDIA AND JAWAHARLAL NEHRU UNIVERSITY, INDIA PROGRAMME The National Symposium on Molecular and Cellular Biophysics is planned with its focus on proteins, nucleic acids and their interactions with other molecules and use of novel and new techniques for biophysical studies. The thematic plan of the symposium is as under: # Structural aspects of biomolecules # Drug design # Protein-DNA, drug-DNA, ligand-membrane interaction # Radiation damage, radiation Biology # Protein-folding, stability and engineering TECHNICAL SESSIONS 1. Drug designing 2. Protein and nucleic acid structures and function 3. Protein-DNA, drug-DNA interaction 4. Membrane Biophysics 5. Radiation biophysics and Cellular Biophysics 6. Protein folding, stability ad engineering 7. Biophysical instrumentation 8. Theoretical Biology Registration Fee Rs. Members IBS 400.00 Non-members 500.00 Students 200.00 Accompanied persons 250.00 NATIONAL SYMPOSIUM ON MOLECULAR AND CELLULAR BIOPHYSICS INDIAN BIOPHYSICAL SOCIETY (IBS) R E G I S T R A T I O N F O R M 1. Name : 2. a) Sex : Male/Female b) Nationality 3. Designation : 4. Address : Fax No. : e-mail : 5. Accommodation needed : Yes/No 6. Bank Draft Amount : Number Date 7. Accompanied by : 8. Any other comments : Date : Signature Note:All payments should be made by demand drafts in the favour of Convenor, National Symposium on molecular and cellular Biophysics, and mailed to Dr. K. Dalal, Assos. Professor Dept. of Biophysics, All India Institute of medical sciences, New Delhi From owner-biophysics@net.bio.net Tue Oct 24 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!uknet!daresbury!not-for-mail From: Newsgroups: bionet.biophysics Subject: quanticell 700 Date: 24 Oct 1995 15:33:13 -0000 Lines: 16 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <46j0vp$s69@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk Dear netters, Can someone help me with the following problem. I know the name of a software package, but I don't know the manufacturer. The software package is named quanticell 700. This is ratio-software for digital imaging. __________________________Rob Ameerun_________________________ Hubrecht Laboratory | tel: ..31-30-510211 Dpt. of Biophysics | fax: ..31-30-516464 Uppsalalaan 8 | E-mail: roba@hubrecht.nl NL-3584-CT Utrecht | The Netherlands | ______________________________________________________________ From owner-biophysics@net.bio.net Tue Oct 24 22:00:00 1995 Newsgroups: bionet.biophysics Path: biosci!bcm.tmc.edu!pendragon.jsc.nasa.gov!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!howland.reston.ans.net!torn!utnut!oci!gc!saleh From: Mazen Saleh Subject: Re: Enzyme In-Reply-To: <46b1oh$o83@nef.ens.fr> X-Sender: saleh@gc Content-Type: TEXT/PLAIN; charset=US-ASCII Message-ID: Sender: news@oci.utoronto.ca Organization: Ontario Cancer Institute - U of Toronto References: <46b1oh$o83@nef.ens.fr> Mime-Version: 1.0 Date: Tue, 24 Oct 1995 12:56:03 GMT Lines: 20 what i know about lipids or fatty acids is that they break down in two carbon units by the fatty acid oxidation cycle. this starts in the charged or "head" end of the lipid.i am not aware of any enzymes that work on the tail of lipids. cheers -mazen On 21 Oct 1995, CHAIEB Sahraoui wrote: > Can anybody tell me , if an enzyme able to cat the tails of a phospholipid > exists, Or one of the tails of the phospholipid, is also interesting. > please reply to my personnal e-mail adress, I'm not subscribed to this > newsgroup > Thanks > my email: chaieb@physique.ens.fr > > > From owner-biophysics@net.bio.net Tue Oct 24 22:00:00 1995 Path: biosci!BTP8X2.PHY.UNI-BAYREUTH.DE!btp849 From: btp849@BTP8X2.PHY.UNI-BAYREUTH.DE (Klaus Fritsch) Newsgroups: bionet.biophysics Subject: (none) Date: 24 Oct 1995 10:19:30 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510241712.SAA10611@ep4> unsubscribe biophys From owner-biophysics@net.bio.net Tue Oct 24 22:00:00 1995 Newsgroups: bionet.biophysics Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!news.dorsai.org!goldfarb From: goldfarb@news.dorsai.org (Sasha Goldfarb) Subject: Antibiotic selection expert system question Message-ID: Sender: news@dorsai.org (Keeper of the News) Organization: The Dorsai Embassy - New York X-Newsreader: TIN [version 1.2 PL2] Date: Wed, 25 Oct 1995 14:53:12 GMT Lines: 1 From owner-biophysics@net.bio.net Tue Oct 24 22:00:00 1995 Path: biosci!CS.Arizona.EDU!news.Arizona.EDU!hamblin.math.byu.edu!acs2.byu.edu!news.cuny.edu!ukma!usenet.eel.ufl.edu!brutus.bright.net!chi-news.cic.net!uwm.edu!lll-winken.llnl.gov!enews.sgi.com!sgigate.sgi.com!sgiblab!cgl!friedman From: friedman@cgl.ucsf.edu (Simon Friedman) Newsgroups: bionet.molbio.proteins,bionet.biophysics Subject: Re: Protein model kits anywhere? Date: 25 Oct 1995 02:11:30 GMT Organization: Computer Graphics Laboratory, UCSF Lines: 74 Message-ID: <46k6ci$i7v@cgl.ucsf.edu> References: <463svi$7l8@decaxp.harvard.edu> <46cj1j$ujo@cgl.ucsf.edu> <46et7s$c5@hermes.cair.du.edu> NNTP-Posting-Host: socrates.ucsf.edu Xref: biosci bionet.molbio.proteins:6091 bionet.biophysics:1353 In article <46et7s$c5@hermes.cair.du.edu> dbovee@phoebe.cair.du.edu (David Bovee) writes: >In article <46cj1j$ujo@cgl.ucsf.edu>, friedman@cgl.ucsf.eduÍ says... >> >>In article <463svi$7l8@decaxp.harvard.edu> berriz@husc.harvard.edu writes: >>> >>>...My question is, do specialized, off-the-shelf kits exist for modelling >>>proteins? (For example, it would be handy if one could get long >>>chunks of ready-made backbone, with all bonds free to rotate, except >>>for the peptide bonds.) If so, who sells them? >>> >> >>Every modeling kit I have seen allows you to model double bonds >>or bonds with double bond character and hindered rotation. In one system >>this is accomplished by having two sticks in parallel form the bond. >>So, in the peptide bond, the amide N and carbonyl C have a stick >>between them, and a second stick that snaps on to the two >>atoms on the side. This allows only small deviations from >>planarity for the bond, which is a fair representation of the >>peptide bond. >>So check out the kit at your bookstore closely. I'm guessing it has >>some sort of provision for modeling double bonds/partial double bonds. >>If no, I can post the name of my kit (which I can't remember off hand). >> >>Simon >> >Generally, this type of physical modeling kit does not allow for such bonds >without using up one of the available bonding sites. For example, modeling a >peptide backbone using a double bond C=N and C=O (which is pretty close to >reality) would use up all four of the bonding sites on the C atom. The only >way that type of kit would be modifiable for the type of backbone which "Z" >wants is if the atoms had sites for more than then are neutrally allowed. > Actually the kits I'm refering to have special pieces for sp2 carbons nitrogens etc. They have 3 valences, plus the ability to add a second bond on the side of them, to two different groups. I'm afraid this is getting too much into discussion and not enough with hard info. I'll check the name of my kit tonight and post tomorrow. Simon From owner-biophysics@net.bio.net Tue Oct 24 22:00:00 1995 Newsgroups: bionet.biophysics Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!news.dorsai.org!goldfarb From: goldfarb@news.dorsai.org (Sasha Goldfarb) Subject: A/b expert system Q, last post went blank Message-ID: Sender: news@dorsai.org (Keeper of the News) Organization: The Dorsai Embassy - New York X-Newsreader: TIN [version 1.2 PL2] Date: Wed, 25 Oct 1995 15:13:16 GMT Lines: 29 Hi all, I would appreciate if anybody can help me with the information on following matter. We are going to develop an expert system for an optimal antibiotic selection (or combination of antibiotics) in patients with peritonitis, mostly in patients on peritoneal dialysis. I did Medline search but did not find anything like that. However the problem is very obvious and seems to be very suitable for expert system approach. My question is, is there is anybody, who knows about a work that has been already done or software (incl. commercial and shareware) that has been already deleloped? Do you think the subject worth efforts? Any other suggestions will be welcomed. Our approach is to develop system that will suggest antibiotics or their combinations in cases of infections, primarily peritonitis, including combined infection. The system planned to be easy updated with new a/b on the market, new data of bacterial resistance, etc. All suggestions are welcomed. Please respond directly to me. Thanks. Sasha Goldfarb. From owner-biophysics@net.bio.net Wed Oct 25 22:00:00 1995 Path: biosci!arl.rdc.toshiba.co.jp!kalim From: kalim@arl.rdc.toshiba.co.jp Newsgroups: bionet.biophysics Subject: Experimental handling of Proteins/Isoelectric Points Date: 25 Oct 1995 20:13:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: <9510260311.AA01120@arl.rdc.toshiba.co.jp> NNTP-Posting-Host: net.bio.net I am new to working with proteins (I will be looking at proteins with the AFM) and have a few basic questions. Where can I find the isolelectric point for different proteins? I`ve tried Merck Index but this does`nt seem to have it for most of the proteins I`m interested in. I am particularly interested in redox proteins. I have a question about handling of Proteins. Is there any general rule for the lifetime of proteins?-There is some cytochrome c sitting around in the fridge which may be two years or more old. Also I am making up Lysozyme solution. Is Lysozyme happy in buffer for an extended period? Does a number of freeze/thawings of Lysozyme crystals have a detrimental effect? Should I take any precautions against proteases? I would be most grateful if someone could help. Kalim Mir From owner-biophysics@net.bio.net Wed Oct 25 22:00:00 1995 Newsgroups: bionet.biophysics Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!spool.mu.edu!torn!blaze.trentu.ca!IVORY.trentu.ca!GMCKENZIE From: gmckenzie@ivory.trentu.ca (NAME) Subject: IGFBP in Mus X-Nntp-Posting-Host: trentu.ca Message-ID: Sender: news@blaze.trentu.ca (USENET News System) Reply-To: gmckenzie@ivory.trentu.ca Organization: Trent University, Peterborough Date: Thu, 26 Oct 1995 17:11:23 GMT Lines: 2 I wish to investigate the Insulin-like growth factor binding protein in Mus. The gist is I desire to analyze amino acid sequences due to codon degeneracy in nucleic acid seqs. Does anyone work with these animals? I want to do an analysis of homology, and seq. alignment and molecular evolution of the gene superfamily via phylip, Swiss-prot et cetera. Any info from individuals in the field would be greatly appreciated. Thanx GMcKenzie@TrentU.Ca From owner-biophysics@net.bio.net Wed Oct 25 22:00:00 1995 Path: biosci!agate!usenet.kornet.nm.kr!news.kreonet.re.kr!news.dacom.co.kr!usenet From: Jongwon Song Newsgroups: bionet.biophysics Subject: tbt restrictions Date: 26 Oct 1995 03:28:15 GMT Organization: DACOM Internet Lines: 11 Message-ID: <46mv8f$3fo@nis.dacom.co.kr> NNTP-Posting-Host: magicall.dacom.co.kr Dear sir This is Jongwon Song from Korea. Could I ask you about a special thing. I want to know whether there are any restrictions on use of tbt(tributyltin) in antifouling coating in North Atlantic sea including the open sea. and What the latest regulation related to tbt is in EU as well as other countries. Please let me know Thank in advance yours sincerely From owner-biophysics@net.bio.net Wed Oct 25 22:00:00 1995 Path: biosci!ICT.SLD.CU!NORMANDO From: NORMANDO@ICT.SLD.CU (Normando Iznaga) Newsgroups: bionet.biophysics Subject: (none) Date: 26 Oct 1995 11:16:17 -0700 Organization: Centro de Inmunologia Molecular Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <9510261451.AA02687@infomed> NNTP-Posting-Host: net.bio.net unsubscribe methods end !*************************************! ! M. Sc. Normando Iznaga Escobar ! ! Center of Molecular Immunology ! ! Division of Antibody Engineering ! ! PO BOX 16040, Havana, 11 600, Cuba ! ! ! ! Fax Number : (53 7) 33 50 49 ! ! (53 7) 33 35 09 ! ! Phone : (53 7) 21 68 11 ! ! (53 7) 21 79 33 ! ! e-mail : normando@ict.sld.cu ! !*************************************! From owner-biophysics@net.bio.net Thu Oct 26 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!blackbush.xlink.net!rz.uni-karlsruhe.de!rzstud1.rz.uni-karlsruhe.de!um0c From: um0c@rzstud1.rz.uni-karlsruhe.de (Michael Maier) Newsgroups: bionet.biophysics Subject: Searching Data for a Dummy Date: 27 Oct 1995 07:17:23 GMT Organization: University of Karlsruhe, Germany Lines: 10 Message-ID: <46q123$hgh@nz12.rz.uni-karlsruhe.de> NNTP-Posting-Host: rzstud1.rz.uni-karlsruhe.de Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit NNTP-Posting-User: um0c X-Newsreader: TIN [version 1.2 PL2] Hello, a friend of mine is searching a dataset for a 8-12 year child dummy. Does anybody know, where he can get this dataset. Thanks a lot for answers. Bye Michael -- Michael Maier *** eMail um0c@rz.uni-karlsruhe.de From owner-biophysics@net.bio.net Thu Oct 26 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!howland.reston.ans.net!newsfeed.internetmci.com!in2.uu.net!hearst.acc.Virginia.EDU!newslink.runet.edu!dmcclain From: dmcclain@runet.edu (Dennis McClain-Furmanski) Newsgroups: bionet.biophysics Subject: Need Advice on Cellular Level Measurements Date: 27 Oct 1995 07:08:52 GMT Organization: Radford University Lines: 34 Message-ID: <46q0i4$evf@newslink.runet.edu> NNTP-Posting-Host: james-gw.runet.edu X-Newsreader: TIN [version 1.2 PL2] If this isn't the correct group, please send along a suggestion to a more appropriate one. I'm interested in looking at very small scale microwave region emissions from areas of neurons. While most such cases would be in the micrometer size, the easiest neurons to work with are much larger. Specifically, I want to examine dendritic fields for emissions of microwaves which might represent the existence of ordered water. If my understanding serves me, this should act as a very small maser, probably at 4.5 or 9 GHz. I need advice on what kinds of equipment I'd need to detect this, with enough resolution to say that this is in fact where it came from. If detected, the next step would involve stimulating the area with like EM waves, and seeing if they instigate an action potential at the axon hillock. Forthe initial work, I expect to need a spatial resolution of .1 to .001 mm. If the physics allows, later it would go much lower. Also, if anyone has references to anything along these lines -- neural/cellular and microwaves -- please share them. I'm aware of the theoretical underpinnings, or present lack. However, I work under someone who's used to working in that fashion, and intend to carry it out regardless. All I'm interested in is the feasibility of the relevant devices at this point, or in developing them if necessary. -=%@%=-=%@%=-=%@%=-=%@%=-=%@%=-=%@%=-=%@%=-=%@%=-=%@%=-=%@%=-=%@%=- < > < dmcclain@runet.edu, Experimental Psychology, Radford University > < Graduate Research Fellow, Center for Behavioral Research and > < INformation Sciences (BRAINS Center) How do you like your EEGs? > < > -=%@%=-=%@%=-=%@%=-=%@%=-=%@%=-=%@%=-=%@%=-=%@%=-=%@%=-=%@%=-=%@%=- From owner-biophysics@net.bio.net Thu Oct 26 22:00:00 1995 Path: biosci!LOVELACE.INFOBIOGEN.FR!picard From: picard@LOVELACE.INFOBIOGEN.FR (Laurent Picard) Newsgroups: bionet.biophysics Subject: lipids and vesicles info wanted Date: 27 Oct 1995 10:38:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510271321.OAA02164@lovelace.infobiogen.fr> NNTP-Posting-Host: net.bio.net Hi, I have a few questions to ask about lipids and vesicles Does anyone knows (1) if the NH2 group of lipids (especially phosphatidyl ethalonamine) is being biotinylated by biotin when labelling cell surface using NHS-ester of sulfo-biotin. (2) Does anyone knows if a sulfo-biotin-labelled lipid is able to flip from one cell to another or from a lipidic vesicle to a cell in presence or in absence of a specific (or non specific) interaction with the cell. (3) does anyone have a protocol to remove aspecific absorbed vesicles (pure lipidic or plasma membrane derived) from cells (acidic washs or chemicals ?). Thanks in advance for your help. Please reply by e-mail Laurent Picard INSERM / U. 332 Institut Cochin de Genetique Moleculaire 22, rue Mechain 75014 Paris, FRANCE E-mail : picard@infobiogen.fr Tel : +33 (1) 40 51 64 96 Fax : +33 (1) 40 51 77 49 From owner-biophysics@net.bio.net Fri Oct 27 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news.u.washington.edu!root From: meir@zoology.washington.edu (Eli Meir) Newsgroups: bionet.biophysics Subject: Biology Education Software FAQ Date: 28 Oct 1995 01:42:48 GMT Organization: Dept. of Zoology, Univ. of Washington Lines: 16 Sender: -Not-Authenticated-[3641] Message-ID: <46s1qo$2l1@nntp3.u.washington.edu> NNTP-Posting-Host: quaternary.zoology.washington.edu X-Posted-From: InterNews 1.0.5@quaternary.zoology.washington.edu Xdisclaimer: No attempt was made to authenticate the sender's name. I have just posted a new version of my Biology Education Software FAQ on bionet.software. You can also download a copy from ftp.zoology.washington.edu /pub/doc Thanks to Rees Griffith and Alan Cairns, there is now also an official htmp version of the FAQ (not yet updated to this latest version, but should be within a few days) posted at http://www-hpcc.astro.washington.edu/scied/bio.html There are also other links to the FAQ scattered around at various web sites. Hope that's useful. Eli Meir Dept of Zoology, Univ of Washington meir@zoology.washington.edu From owner-biophysics@net.bio.net Sun Oct 29 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!lll-winken.llnl.gov!enews.sgi.com!sgigate.sgi.com!sgiblab!cgl!friedman From: friedman@cgl.ucsf.edu (Simon Friedman) Newsgroups: bionet.molec-model,bionet.biophysics Subject: Model Kit that gives planer peptide bonds Date: 30 Oct 1995 02:44:54 GMT Organization: Computer Graphics Laboratory, UCSF Lines: 13 Sender: friedman@cgl.ucsf.edu (Simon Friedman) Distribution: world Message-ID: <471e76$ecb@cgl.ucsf.edu> NNTP-Posting-Host: socrates.ucsf.edu Xref: biosci bionet.molec-model:638 bionet.biophysics:1371 This is for the person who was looking for a molecular modeling kit that would allow planer peptide bonds. The kit I have which allows this is the "Orbit Molecular Building System" made by "Cochranes of Oxford, Fairspear House, Leafield, Oxford, UK." I don't have the original box, but I believe it was sold through Aldrich at one point. Simon From owner-biophysics@net.bio.net Sun Oct 29 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!usenet.eel.ufl.edu!tank.news.pipex.net!pipex!lade.news.pipex.net!pipex!news00.sunet.se!sunic!psinntp!psinntp!psinntp!psinntp!pfizergate!holly.sandwich.pfizer.com!siris7!jpo From: jpo@siris7 (John Overington) Newsgroups: bionet.molec-model,bionet.molbio.proteins,bionet.xtallography,bionet.structural-nmr,bionet.biophysics,sci.chem,sci.comp-aided Subject: Internet Drug Design Course Date: 30 Oct 1995 08:57:28 GMT Organization: Pfizer Central Research, Sandwich, Kent, UK Lines: 27 Message-ID: <47241o$1r3@holly.sandwich.pfizer.com> NNTP-Posting-Host: siris7.sandwich.pfizer.com Keywords: Internet Drug Design Course X-Newsreader: TIN [version 1.2 PL2] Xref: biosci bionet.molec-model:639 bionet.molbio.proteins:6132 bionet.xtallography:2130 bionet.structural-nmr:857 bionet.biophysics:1372 sci.chem:42204 sci.comp-aided:926 We (Peter Murray-Rust of Glaxo Wellcome, and John Overington of Pfizer) would like to announce an initiative in industrial training for medicinal chemists interested in applying protein structure-based drug design (SBDD) techniques as part of their jobs. Additionally, skills in effectively using the internet as a scientific resource will also be developed. The course will use self-paced, internet-based training, coupled to close contact with course 'tutors', working in the pharmaceutical industry. We are currently looking for further partners to offer financial support, and course material. Partners need to have a presence in the UK to qualify for potential funding under a Technology Foresight grant; although the basic course material will be generally accessible over the internet to the wider community. Further details, and a draft prospectus can be found at http://www.cryst.bbk.ac.uk/SBDD/course.html If you have any further questions, we would be pleased to answer them. John Overington jpo@guitar.rockefeller.edu Peter Murray-Rust ubcg09q@cryst.bbk.ac.uk -- Dr. John Overington email: overingtonj@pfizer.com Pfizer Central Research phone: +44-(0)1304-618467 Sandwich, Kent, CT13 9NJ fax: +44-(0)1304-618422 United Kingdom cc-mail: overington_j@sandwich.pfizer.com From owner-biophysics@net.bio.net Mon Oct 30 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.sprintlink.net!noc.netcom.net!usenet.eel.ufl.edu!tank.news.pipex.net!pipex!soap.news.pipex.net!pipex!usenet From: M J Geisow Newsgroups: bionet.biophysics Subject: PERSPECTIVES ON PROTEIN ENGINEERING: Deadline for early registration Date: 31 Oct 1995 08:06:39 GMT Organization: BIODIGM Ltd. Lines: 24 Message-ID: <474lef$qim@soap.news.pipex.net> NNTP-Posting-Host: am189.du.pipex.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1 (Windows; I; 16bit) PERSPECTIVES ON PROTEIN ENGINEERING MONTPELLIER, FRANCE March 2-6 1996 Reduced (early) registration ends 31.10.95 Information and on-line registration: http://www.cryst.bbk.ac.uk/CEC/pope5.html Main scientific programme published: http://www.cryst.bbk.ac.uk/CEC/program.html List of planned EU Framework III Structural Biology satellite meetings now available: http://www.cryst.bbk.ac.uk/CEC/networks.html Proceedings from the 1994 Oxford Perspectives on Protein Engineering conference now published: http://www.cryst.bbk.ac.uk/CEC/book.html -- BIODIGM Tel: +44 (0) 1949 839077 Fax: +44 (0) 1949 831886 Email: biodigm@dial.pipex.com From owner-biophysics@net.bio.net Mon Oct 30 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news From: Brian B. Jiang <73344.437@CompuServe.COM> Newsgroups: bionet.biology.n2-fixation,bionet.biology.tropical,bionet.biophysics,bionet.celegans,bionet.cellbiol Subject: Anti-Immigration Law Status/Seminar Date: 31 Oct 1995 08:29:34 GMT Organization: The Law Office of Brian B. Jiang Lines: 105 Message-ID: <474mpe$9r9$2@mhafm.production.compuserve.com> Xref: biosci bionet.biology.n2-fixation:390 bionet.biology.tropical:1401 bionet.biophysics:1375 bionet.celegans:641 bionet.cellbiol:3296 RE: New Development On the Anti-Immigration Bill(HR 2202) and Upcoming Seminar Hi folks: As of today, the House Judiciary Committee has completed its markup on the Lamar Bill after 9 days of debate over several weeks. Despite all the bad news, such as reduction of legal immigration and elimination of some family categories, there are a few signs of relief. Both outstanding researchers/professors and national interest waivers were restored to the Bill. So professionals( scientists, post-docs, engineers etc.) can still apply for permanent residence without labor certification and sponsorship of their employers. On the other hand, for those who have to apply through labor cert, they are, as usual, still at the mercy of the Labor Dept. Any positive change is yet to be seen. Although waiver of labor certification and employer sponsorship is still obtainable for those who qualify, difficulties have been on the rise and more are expected. Nearly all four Service Centers have implemented some newly proposed requirements that make it very difficult to obtain an approval. According to administrative rules no law should be carried out until it is final. Despite the rules, INS is already treating the proposals like enforceable law. In the real world, things are not always the way they are supposed to be. This is even more so in immigration practice with today's anti-immigration fervor. I am an immigration attorney specializing in employment based immigration(1st and 2nd preference). I will hold seminars on how to apply for a green card without labor certification and sponsorship of the employer. The intended audiences of my seminar are professionals with advanced degrees or a bachelor degree plus five years of work experience. I will also cover the impact of HR 2202 on future immigration. The locations of the seminar will include: 1. University of Florida at Gainesville University Center Hotel (President's Ballroom) 1535 SW Archer Road Gainesville, FL Tel: (904)371-3333 Time: 11/12/95 Sunday 2:00-4:00pm 2. Columbia University at New York City Columbia University 517 Hamilton Hall (close to W. 116th St. & Broadway) Time: 11/13/95 Monday 7:30-9:30pm 3. Princeton University Location to be decided Time 11/14/95 Tuesday 4. Baltimore near University of Maryland Holiday Inn Baltimore Inner Harbor (Chesateake Ballroom #1) 301 W. Lombard Street Baltimore, MD Tel: (410)685-3500 Time: 11/15/95 Wed. 7:30-9:30pm 5. Pittsburgh near Univ. of Pittsburgh Pittsburgh Green Tree Marriott (Near University of Pittsburgh) 101 Marriott Drive Pittsburgh, PA Tel: (412)922-8400 Time: 11/16/95 Thursday 7:30-9:30pm 6. Boston at Cambridge Hyatt Regency Cambridge 575 Memorial Drive Cambridge, MA Tel: (617)492-1234 Time 11/17/95 Friday 7:30-9:30pm 7. University of Connecticut Location to be decided Time 11/18/95 Saturday 2:30-4:30pm 8. Palo Alto near Stanford University Holiday Inn Palo Alto (Near Stanford University) 625 El Camino Real Palo Alto, CA Tel: (415)328-2800 Time 11/19/95 Sunday 7:30-9:30pm I will conduct these seminars during these time. All questions, comment, and suggestions are welcome. You can also reach me at tel. (619)278-5480 or (619)278-5492. Brian B. Jiang Esq. Email: 73344.437@compuserve.com