From owner-biophysics@net.bio.net Wed Nov 01 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!hodes.com!netcomsv!uu4news.netcom.com!netcomsv!uu3news.netcom.com!ix.netcom.com!netnews From: mbmorton@ix.netcom.com (michelle morton ) Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.general,bionet.microbiology,bionet.molbio.proteins Subject: biophysics vs. biochem speculatin'.... Date: 2 Nov 1995 11:00:40 GMT Organization: Netcom Lines: 26 Message-ID: <47a8co$3s5@ixnews2.ix.netcom.com> NNTP-Posting-Host: ix-lb5-12.ix.netcom.com X-NETCOM-Date: Thu Nov 02 3:00:40 AM PST 1995 Xref: biosci bionet.biophysics:1379 bionet.cellbiol:3318 bionet.general:18250 bionet.microbiology:3810 bionet.molbio.proteins:6165 Got a question that's been puzzling me. My cat's fur is so soft that petting him is like touching a cashmere or angora sweater. My question is this - is *softness* due to: a) the **physical** arrangement of the peptide bonds - do the bonds and the amino acids they're attached to form a smooth, rather than jagged or discrete, surface? b) the tertiary or quarternary folding of the protein(s) in his fur - ie, are the proteins physically arranged in some special way so that they are experienced by us as being *soft*? c) the sequencing of the amino acids themselves, or d) some other phenomenom that I haven't thought of and probably can't even imagine right now? Obviously, as a student I have wwwaaaaaayyyy too much time on my time on my hands :>!!! This is one of several "philosophy" of chemistry questions I have that keep me from ever getting around to figuring out how to solve world hunger or bring about total global peace on earth and other really important stuff like that! Soooooooo, if you can help me out, I'd really appreciate it. Thanx in advance for your info. michelle (my mind's awanderin', awanderin', just awanderin' away) m From owner-biophysics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!news.math.psu.edu!psuvax1!news.eecs.nwu.edu!newsfeed.acns.nwu.edu!news.acns.nwu.edu!lucky139.acns.nwu.edu!user From: george-munson@nwu.edu (George Munson) Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.general,bionet.microbiology,bionet.molbio.proteins Subject: Re: biophysics vs. biochem speculatin'.... Date: Thu, 02 Nov 1995 21:30:34 -0600 Organization: Northwestern University Lines: 29 Message-ID: References: <47a8co$3s5@ixnews2.ix.netcom.com> NNTP-Posting-Host: lucky139.acns.nwu.edu Xref: biosci bionet.biophysics:1380 bionet.cellbiol:3323 bionet.general:18254 bionet.microbiology:3820 bionet.molbio.proteins:6173 > Got a question that's been puzzling me. My cat's fur is so soft that > petting him is like touching a cashmere or angora sweater. My question > is this - is *softness* due to: > > a) the **physical** arrangement of the peptide bonds - do the bonds and > the amino acids they're attached to form a smooth, rather than jagged > or discrete, surface? > > b) the tertiary or quarternary folding of the protein(s) in his fur - > ie, are the proteins physically arranged in some special way so that > they are experienced by us as being *soft*? > > c) the sequencing of the amino acids themselves, or > > d) some other phenomenom that I haven't thought of and probably can't > even imagine right now? Hmm. Okay this is my best guess and I chose "b." I doubt tactile perception is sensitive enough to distinguish peptide bonds or aa sequence per se. What you perceive is the much larger scale of the hair which is of course ultimately a result of c, a, and b. My guess is softness is related to rigidity or the lack thereof. Rigidity of the overall hair would likely arise from intermolecular disulfide cross linkages which would be tertiary/quartenary in nature. -- George Munson BMBCB, Northwestern University Evanston, IL USA george-munson@nwu.edu From owner-biophysics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!pendragon.jsc.nasa.gov!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!noc.near.net!das-news2.harvard.edu!fas-news.harvard.edu!fas.harvard.edu!berriz From: Zorro Newsgroups: bionet.biophysics,bionet.molbio.proteins Subject: HELP: simple graphics/animation job Date: 3 Nov 1995 13:40:45 GMT Organization: Harvard University, Cambridge, Massachusetts Lines: 22 Message-ID: <47d64t$8q2@decaxp.harvard.edu> Reply-To: berriz@husc.harvard.edu NNTP-Posting-Host: fas.harvard.edu Keywords: animation engines, players(?) X-Newsreader: NN version 6.5.0 #3 (NOV) Originator: berriz@fas.harvard.edu Xref: biosci bionet.biophysics:1387 bionet.molbio.proteins:6180 Hello. I'm a researcher in molecular dynamics (and all but illiterate when it comes to computer graphics). I do computer simulations of biopolymer dynamics on an IBM RISC System 6000 machine running AIX. It would be to me very useful to crudely visualize these simulations. I can easily produce tons of "frames" consisting of the 3D coordinates of each monomer of the simulated polymer chain, as the simulation unfolds. (Of course, from these coordinates, a crude picture of the chain can be obtained by "connecting the dots" with line segments.) What I need is a program that will sequentially display a time-series of such frames. I'm hoping to find freely available source code to do this. I would greatly appreciate information on FTP sites and WWW pages where I may be able to find such tools. Thank you. Z. From owner-biophysics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!newsfeed.ACO.net!ludens.elte.hu!marta From: marta@ludens.elte.hu Newsgroups: bionet.jobs.wanted,bionet.jobs,bionet.biophysics Subject: I need help with PhD studies in Japan Message-ID: <1995Nov3.143050.19493@ludens> Date: 3 Nov 95 14:30:50 +0200 Organization: Eotvos University, Budapest, Hungary Lines: 11 Xref: biosci bionet.jobs.wanted:3116 bionet.biophysics:1386 i need help in getting a PhD scholarship in Japan. My name is Marta Pocsik. i study biophysics in Budapest, Hungary. i do my diploma work on creating mutagenesis and examining the resulting proteins in E. coli. Our goal is to determine the structural properties responsible for the thermal stability of proteins. i obtain my diploma in June, 1996. i would like to start my studies in the autumn of 1996. i would like to know if it is possible to get financial support. If you have any information, please contact me. Thank you very much for your help. Sincerely yours, Marta Pocsik my address is: marta@ludens.elte.hu From owner-biophysics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!newsfeed.ACO.net!sbg.ac.at!news From: Thomas HEISTRACHER Newsgroups: bionet.molec-model,bionet.molbio.proteins,bionet.xtallography,bionet.structural-nmr,bionet.biophysics,sci.chem,sci.comp-aided Subject: Re: Internet Drug Design Course Date: 3 Nov 1995 09:42:45 GMT Organization: uni.sbg Lines: 30 Message-ID: <47co6l$dt3@dwst13.edvz.sbg.ac.at> References: <47241o$1r3@holly.sandwich.pfizer.com> NNTP-Posting-Host: dbio26.bio.sbg.ac.at Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 32bit) To: hartla@edvz.sbg.ac.at Xref: biosci bionet.molec-model:650 bionet.molbio.proteins:6177 bionet.xtallography:2138 bionet.structural-nmr:874 bionet.biophysics:1384 sci.chem:42399 sci.comp-aided:929 jpo@siris7 (John Overington) wrote: > We (Peter Murray-Rust of Glaxo Wellcome, and John Overington of Pfizer) >would like to announce an initiative in industrial training for medicinal >chemists interested in applying protein structure-based drug design (SBDD) >techniques as part of their jobs. Additionally, skills in effectively using >the internet as a scientific resource will also be developed. The course will >use self-paced, internet-based training, coupled to close contact with >course 'tutors', working in the pharmaceutical industry. > > We are currently looking for further partners to offer financial support, >and course material. Partners need to have a presence in the UK to qualify for >potential funding under a Technology Foresight grant; although the basic >course material will be generally accessible over the internet to the wider >community. > > Further details, and a draft prospectus can be found at >http://www.cryst.bbk.ac.uk/SBDD/course.html > > If you have any further questions, we would be pleased to answer them. > > John Overington jpo@guitar.rockefeller.edu > Peter Murray-Rust ubcg09q@cryst.bbk.ac.uk > >-- >Dr. John Overington email: overingtonj@pfizer.com >Pfizer Central Research phone: +44-(0)1304-618467 >Sandwich, Kent, CT13 9NJ fax: +44-(0)1304-618422 >United Kingdom cc-mail: overington_j@sandwich.pfizer.com From owner-biophysics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!news.math.psu.edu!psuvax1!news.eecs.nwu.edu!newsfeed.acns.nwu.edu!movietone.ils.nwu.edu!news.ecn.bgu.edu!vixen.cso.uiuc.edu!newsrelay.iastate.edu!hobbes.physics.uiowa.edu!news.uiowa.edu!red.weeg.uiowa.edu!cgreiner From: "C. Greiner" Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.general,bionet.microbiology,bionet.molbio.proteins Subject: Re: biophysics vs. biochem speculatin'.... Date: Fri, 3 Nov 1995 01:39:37 -0600 Organization: University of Iowa, Iowa City, IA, USA Lines: 36 Distribution: world Message-ID: References: <47a8co$3s5@ixnews2.ix.netcom.com> NNTP-Posting-Host: red.weeg.uiowa.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: cgreiner@red.weeg.uiowa.edu In-Reply-To: Xref: biosci bionet.biophysics:1383 bionet.cellbiol:3328 bionet.general:18260 bionet.microbiology:3826 bionet.molbio.proteins:6176 On Thu, 2 Nov 1995, George Munson wrote: > > a) the **physical** arrangement of the peptide bonds - do the bonds and > > the amino acids they're attached to form a smooth, rather than jagged > > or discrete, surface? > > > > b) the tertiary or quarternary folding of the protein(s) in his fur - > > ie, are the proteins physically arranged in some special way so that > > they are experienced by us as being *soft*? > > > > c) the sequencing of the amino acids themselves, or > > > > d) some other phenomenom that I haven't thought of and probably can't > > even imagine right now? > > Hmm. Okay this is my best guess and I chose "b." I doubt tactile > perception is sensitive enough to distinguish peptide bonds or aa sequence > per se. What you perceive is the much larger scale of the hair which is > of course ultimately a result of c, a, and b. My guess is softness is > related to rigidity or the lack thereof. Rigidity of the overall hair > would likely arise from intermolecular disulfide cross linkages which > would be tertiary/quartenary in nature. > -- > George Munson > BMBCB, Northwestern University > Evanston, IL USA > george-munson@nwu.edu > Although I agree (mostly) with George I wonder if the answer is not "d" with the "softness" relating to the degree of secretion of oils onto the hair rather than the actual molecular structure of the hair itself. Thus the reason high price furs are often from water associated mammals (oil serves as a water barrier) e.g. beaver, mink, fur seal, otter, etc... From owner-biophysics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news1.digital.com!decwrl!lll-winken.llnl.gov!uwm.edu!vixen.cso.uiuc.edu!ux4.cso.uiuc.edu!f-omana From: f-omana@ux4.cso.uiuc.edu (Francis Oliver Omana) Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.general,bionet.microbiology,bionet.molbio.proteins Subject: Re: biophysics vs. biochem speculatin'.... Date: 3 Nov 1995 08:22:18 GMT Organization: University of Illinois at Urbana Lines: 64 Message-ID: <47cjfq$t3q@vixen.cso.uiuc.edu> References: <47a8co$3s5@ixnews2.ix.netcom.com> NNTP-Posting-Host: ux4.cso.uiuc.edu Xref: biosci bionet.biophysics:1382 bionet.cellbiol:3327 bionet.general:18258 bionet.microbiology:3825 bionet.molbio.proteins:6175 mbmorton@ix.netcom.com (michelle morton ) writes: >Got a question that's been puzzling me. My cat's fur is so soft that >petting him is like touching a cashmere or angora sweater. My question >is this - is *softness* due to: >a) the **physical** arrangement of the peptide bonds - do the bonds and >the amino acids they're attached to form a smooth, rather than jagged >or discrete, surface? >b) the tertiary or quarternary folding of the protein(s) in his fur - >ie, are the proteins physically arranged in some special way so that >they are experienced by us as being *soft*? >c) the sequencing of the amino acids themselves, or >d) some other phenomenom that I haven't thought of and probably can't >even imagine right now? >Obviously, as a student I have wwwaaaaaayyyy too much time on my time >on my hands :>!!! This is one of several "philosophy" of chemistry >questions I have that keep me from ever getting around to figuring out >how to solve world hunger or bring about total global peace on earth >and other really important stuff like that! Soooooooo, if you can help >me out, I'd really appreciate it. Thanx in advance for your info. Cats. I'm allergic to cats. But I think they're cute, so I play with them anyway. My friends all have short-hair cats, which means their hairs are kinda fragile and break off. These fragments are also light and have a tendency to get caught in my nasal passages and cause a huge response by my body's immune system. That is the reason I like long-hair cats better. Their hair is much more sturdy and remains in long strands when they break; the hair stays on the floor, know what I mean? Now about cat hair...I haven't read any articles about cat hair, but I do know something about human hair..its supercoiled alpha-helical structure, for instance. Human hair breaks and has split ends sometimes too if the supercoils start separating. Hmmm...guess I should start using conditioner to avoid having *gasp* Damaged hair! "hair, you're damaged goods." I'd say. Hair conditioner....what a concept. Back in the old days, did anyone have shiny, bouncy, silky hair like what you get with a vial of VO5? Maybe that's why they wore bonnets and hats in those 19th century photos? Read the ingredients on a bottle of conditioner. They're mostly emulsions of some type or other, and what I think they do (i don't make the product, i can only hypothesize) is bind to hair and keep the hair strands from linking to each other. Penetrating through and coating the hair, the molecules may provide a more uniform surface than the supercoils alone. then, as you stroke the hair with your fingers, you don't detect roughness on the strands. Furthermore, these molecules may protect the supercoils from being damaged physically, chemically or by the sun's radiation. Water content also plays a role in softness. Ask someone who uses fabric softener! Better yet, which is softer -- a dry towel or a damp one? have you ever left clothes in a dryer for too long? Well? HAVE YOU? So, to summarize. your cat's fur is soft probably bacause its hairs are coated with a natural substance that protects the supercoiled alpha helices from damage, and/or your cat's hairs retain moisture well. F.O. Biophysicist by day, lab vampire at night f-omana@students.uiuc.edu From owner-biophysics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!rutgers!gatech!stallion.jsums.edu!news.uoregon.edu!tank.news.pipex.net!pipex!dispatch.news.demon.net!demon!news.sprintlink.net!wizard.pn.com!Germany.EU.net!EU.net!newsfeed.internetmci.com!chi-news.cic.net!simtel!lll-winken.llnl.gov!usenet From: Chris Barry Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.general,bionet.microbiology,bionet.molbio.proteins Subject: Re: biophysics vs. biochem speculatin'.... Date: 3 Nov 1995 05:08:41 GMT Organization: Lawrence Livermore National Laboratory Lines: 2 Message-ID: <47c84p$s85@lll-winken.llnl.gov> References: <47a8co$3s5@ixnews2.ix.netcom.com> NNTP-Posting-Host: mackiller.llnl.gov Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; Linux 1.1.72 i486) X-URL: news:47a8co$3s5@ixnews2.ix.netcom.com Xref: biosci bionet.biophysics:1381 bionet.cellbiol:3326 bionet.general:18257 bionet.microbiology:3824 bionet.molbio.proteins:6174 Have you wased your cat lately? From owner-biophysics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!pendragon.jsc.nasa.gov!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!simtel!lll-winken.llnl.gov!uwm.edu!vixen.cso.uiuc.edu!news.ecn.bgu.edu!usenet.ins.cwru.edu!peds22060.GENE.CWRU.Edu!rxw13 From: rxw13@po.cwru.edu (Dr. Ron) Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.general,bionet.microbiology,bionet.molbio.proteins Subject: Re: biophysics vs. biochem speculatin'.... Date: Fri, 3 Nov 1995 11:41:32 E.S.T. Organization: Case Western Reserve University, Cleveland OH (USA) Lines: 27 Message-ID: References: <47a8co$3s5@ixnews2.ix.netcom.com> NNTP-Posting-Host: peds22060.gene.cwru.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev B] Xref: biosci bionet.biophysics:1390 bionet.cellbiol:3333 bionet.general:18268 bionet.microbiology:3834 bionet.molbio.proteins:6186 > mbmorton@ix.netcom.com (michelle morton ) writes: >Got a question that's been puzzling me. My cat's fur is so soft that >petting him is like touching a cashmere or angora sweater. My question >is this - is *softness* due to: ... >Obviously, as a student I have wwwaaaaaayyyy too much time on my time >on my hands :>!!! This is one of several "philosophy" of chemistry >questions I have that keep me from ever getting around to figuring out >how to solve world hunger or bring about total global peace on earth >and other really important stuff like that! Soooooooo, if you can help >me out, I'd really appreciate it. Thanx in advance for your info. Never apologize for thinking, Mich. Lotsa good ideas have their origins in idle thoughts. I don't have an answer, but a speculation -- 'softness' in fur is a lack of resistance to pressure, yet a resilience once that pressure is removed. Probalby all of your options are involved -- protein tertiery and quaternary structure that permits easy alignment of individual hairs, possibly a thin monomolecular film lanolin coating that prevents electrostatic interactions between strands - but isn't thick enough to feel greasy or allow stickiness. It might be interesting for a industrial polymer company to find out what ARE the differences between angora-y soft cats and plain ordinary cats at the molecular and physical levels, if they already haven't -- presumably, preety much the same structural proteins -- maybe differences in proline content? From owner-biophysics@net.bio.net Thu Nov 02 22:00:00 1995 Newsgroups: bionet.biophysics Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!hodes.com!netcomsv!uu4news.netcom.com!netcomsv!uu3news.netcom.com!ix.netcom.com!netcom.com!tday.slip.netcom.com!user From: tday@netcom.com (Tony Day) Subject: Help needed with Hill Equn. Message-ID: Sender: netnews@mork.netcom.com Nntp-Posting-Host: tday.slip.netcom.com Organization: NETCOM On-line Communication Services (408 261-4700 guest) Date: Fri, 3 Nov 1995 03:50:08 GMT Lines: 17 We have a protein which is destabilised by a small molecule. The effect saturates at low mM concentrations. The argument in the lab is whether it is non-specific or specific. I measured a series of inactivation rates at different inactivator concentrations. The data were fitted to a non-linear form of the Hill Eqn.. This gave a good fit with a Hill coefficient of 1.02. at a Kd of ~.8mM. Also the residuals looked random. However, I am a novice at using the Hill Equn. My question is as follows: Is there any other reasonable interpretation of the good fit and Hill coefficient of 1, than a specific single binding site (or identical sites)? In particular, could it plausibly be non-specific binding? Many thanks in advance. Tony Day From owner-biophysics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!MVAX1.RED.CINVESTAV.MX!AGUERRER From: AGUERRER@MVAX1.RED.CINVESTAV.MX ("AGUSTIN GUERRERO HERNANDEZ") Newsgroups: bionet.biophysics Subject: Newsgroup on Calcium Date: 3 Nov 1995 07:51:59 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511031551.HAA02459@net.bio.net> NNTP-Posting-Host: net.bio.net Hi Biophysics netters: I have been wondering if there is a newsgroup on Calcium out there? Ideally focused on calcium as a second messenger from Ca channels to Ca effectors. Initially I thouhgt that this newsgroup could have been the right one but my survey for a couple of months tells me that maybe not...(if I want to know how to become rich overnight, learn to cook or have sex by phone, this list seems to be the right one...). So, if you have any information of mailing list of people interested in calcium as second messenger I would like to become a member so any information as to how to do that will be appreciated. Agustin Guerrero, Ph. D. Associate Professor Dept. of Biochemistry CINVESTAV-IPN Mexico tel (525)747-7000 x 5210 FAX (525)747-7083 aguerrer@mvax1.red.cinvestav.mx From owner-biophysics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!uwm.edu!lll-winken.llnl.gov!enews.sgi.com!decwrl!news-server.ncren.net!news.duke.edu!usenet From: "Dr. David Rosen" Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.general,bionet.microbiology,bionet.molbio.proteins Subject: Re: biophysics vs. biochem speculatin'.... Date: 3 Nov 1995 14:47:01 GMT Organization: Duke University, Durham, NC, USA Lines: 11 Message-ID: <47da15$2is@news.duke.edu> References: <47a8co$3s5@ixnews2.ix.netcom.com> <47cjfq$t3q@vixen.cso.uiuc.edu> NNTP-Posting-Host: inxs.chem.duke.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; SunOS 4.1.2 sun4c) X-URL: news:47cjfq$t3q@vixen.cso.uiuc.edu Xref: biosci bionet.biophysics:1391 bionet.cellbiol:3334 bionet.general:18269 bionet.microbiology:3835 bionet.molbio.proteins:6187 I think the "moisture" that keeps hair "soft and manageable" is a lipid (oil) rather than water. Lanolin is one oil that keeps hair slippery. I think the physical structure of the hair may explain other things in the "feel" of the fur. Thick hair strands feel different from fine fur strands. Incidently, I don't think anyone should apologize for asking questions like this. Questions asked for "idle" curiousity end up being more important than "relevant" questions. Basic research is in the long run more useful than applied research. I suggest that our student NOT take any answers for granted, make a project out of testing some hypothesis, and perhaps make a bundle off of Lady Clairol. From owner-biophysics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!lll-winken.llnl.gov!usenet From: Chris Barry Newsgroups: bionet.biophysics Subject: Microspheres Date: 3 Nov 1995 22:49:01 GMT Organization: Lawrence Livermore National Laboratory Lines: 14 Message-ID: <47e68t$3i8@lll-winken.llnl.gov> NNTP-Posting-Host: mackiller.llnl.gov Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; Linux 1.1.72 i486) X-URL: news:bionet.biophysics Hello, I was wondering if anyone has a protocall for attaching protein (IgG's) to carboxylated microspheres. If so, I would be gratefull if you could forward a copy to me. I would also appreciate any vendors of the microspheres. Thanks in advance. Chris Barry LLNL 7000 East Ave. L-452 Livermore, CA 94550 chbarry@mackiller.llnl.gov From owner-biophysics@net.bio.net Fri Nov 03 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!chi-news.cic.net!newsfeed.internetmci.com!in1.uu.net!newsfeed.pitt.edu!news From: "M.L. Raghavan" Newsgroups: bionet.biophysics Subject: NSF-Japan program for engineering Date: 4 Nov 1995 06:48:44 GMT Organization: University of Pittsburgh Lines: 21 Message-ID: <47f2cc$f2g@usenet.srv.cis.pitt.edu> NNTP-Posting-Host: ehdup-c2-3.rmt.net.pitt.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: quoted-printable X-Mailer: Mozilla 1.22 (Windows; I; 16bit) To: marta@ludens.elte.hu marta@ludens.elte.hu wrote: >i need help in getting a PhD scholarship in Japan. i would like to know >if it is possible to get financial support. If you have an= y information, >please contact me. >Thank you very much for your help. Sincerely yours, Marta Pocsik > >my address is: marta@ludens.elte.hu Check out ftp://cs.arizona.edu/japan/nsf/ Its an NSF-Japan colloboration for internship for US grad students! You might get started on your search from there. Good Luck M.L. Raghavan From owner-biophysics@net.bio.net Sat Nov 04 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!news.fyionline.com!op.net!en.com!usenet From: Jose Nazario Newsgroups: bionet.biophysics Subject: Re: Where can I download RASMOL2 from? Date: 5 Nov 1995 06:54:45 GMT Organization: none Lines: 9 Message-ID: <47hn3l$hqs@antares.en.com> References: <46b4c5$e6m@decaxp.harvard.edu> NNTP-Posting-Host: dial-40.en.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: berriz@husc.harvard.edu X-URL: news:46b4c5$e6m@decaxp.harvard.edu i just got a copy of rasmol2 (v2.5 for the mac, actually) from ftp.pdb.bnl.gov (/pub/other-software i think). anyhow, source and compiled versions are available there for rasmol. jose nazario nazario@en.com case western reserve university From owner-biophysics@net.bio.net Sun Nov 05 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!uwm.edu!lll-winken.llnl.gov!usenet From: Chris Barry Newsgroups: bionet.biophysics Subject: Re: Where can I download RASMOL2 from? Date: 5 Nov 1995 23:02:41 GMT Organization: Lawrence Livermore National Laboratory Lines: 4 Message-ID: <47jfqh$r80@lll-winken.llnl.gov> References: <46b4c5$e6m@decaxp.harvard.edu> <47hn3l$hqs@antares.en.com> NNTP-Posting-Host: mackiller.llnl.gov Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; Linux 1.1.72 i486) X-URL: news:47hn3l$hqs@antares.en.com From: http://xerxes.crbm.cnrs-mop.fr/Rasmol/rasmol.html From owner-biophysics@net.bio.net Sun Nov 05 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!EU.net!uknet!yama.mcc.ac.uk!zippy.dct.ac.uk!dundee.ac.uk!ee20.apeme.dundee.ac.uk!crabier Newsgroups: bionet.biophysics Subject: Electrical measurements on bacteria using Microelectronics Message-ID: From: crabier@its.dundee.ac.uk (CELINE RABIER) Date: Mon, 6 Nov 1995 10:59:49 GMT Organization: University of Dundee NNTP-Posting-Host: ee20.apeme.dundee.ac.uk X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Lines: 9 Hi everyone! I am a final year student in Electronic and Electrical Engineering and would need any kind of information regarding electric/impedance measurements on bacteria and protozoa (microorganisms) using Microelectronic techniques... If you can help me, please contact me as quickly as possible!!! Hope to hear from you soon! Celine From owner-biophysics@net.bio.net Sun Nov 05 22:00:00 1995 Path: biosci!ns1.faseb.org!lamarck.sura.net!newsfeed.internetmci.com!howland.reston.ans.net!newsjunkie.ans.net!inet.d48.lilly.com!sherwood.d46.lilly.com!gfn Newsgroups: bionet.biophysics Subject: Industrial Job Opening-Now Message-ID: <1995Nov6.080305.6721@inet.d48.lilly.com> From: Greg Needham Date: 6 Nov 95 08:03:05 EST Distribution: world Organization: Eli Lilly and Co. Nntp-Posting-Host: sherwood.d46.lilly.com X-UserAgent: Version 1.1.3 X-XXMessage-ID: X-XXDate: Mon, 6 Nov 95 08:03:52 GMT Lines: 18 Associate Formulations Chemist. An associate formulations chemist is needed for the development and characterization of formulations for new animal health products. This scientist will provide laboratory support for the identification of new dosage forms or delivery systems for synthetic organic compounds and fermentation products. Preference will be given to qualified candidates having a BS or MS degree and relevant experience in physical chemistry, chemical engineering, pharmaceutics, pharmaceutical sciences, or a related field. Interested individuals should please respond with a resume to: Mr. Doug Whiteman Lilly Research Laboratories 2001 West Main Street Greenfield, IN 46140 Eli Lilly and Company is an equal opportunity employer. ------------------------------------------------------------------------ From owner-biophysics@net.bio.net Sun Nov 05 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!btnet!dispatch.news.demon.net!demon!intergen.demon.co.uk!angus From: angus@intergen.demon.co.uk (Angus Cameron) Newsgroups: bionet.molecules.peptides,bionet.biophysics,bionet.cellbiol.cytonet,bionet.diagnostics.prenatal,bionet.glycosci,bionet.microbiology,bionet.molecules.peptides Subject: A senior sales/management position exists in Europe Date: Mon, 6 Nov 1995 08:33:40 Organization: Intergen Lines: 6 Message-ID: NNTP-Posting-Host: intergen.demon.co.uk Summary: A German speaking manager is wanted to run a European biotech. business Keywords: Sales, Biopharmaceutical, Diagnostics X-NNTP-Posting-Host: intergen.demon.co.uk X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Xref: biosci bionet.molecules.peptides:92 bionet.biophysics:1397 bionet.cellbiol.cytonet:366 bionet.diagnostics.prenatal:66 bionet.glycosci:437 bionet.microbiology:3861 A US manufacturing company is looking for a German speaking manager to develop its business of the supply of sophisticated manufacturing raw materials to the biopharm. and diagnostic businesses in Europe. Pref. UK based with access to Oxford. Very interesting opportunity indeed for an individual from the above background. From owner-biophysics@net.bio.net Sun Nov 05 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!btnet!dispatch.news.demon.net!demon!mail2news.demon.co.uk!dfnserv1.urz.uni-magdeburg.de From: Tino J ger Newsgroups: bionet.biophysics Subject: hippocampal slice culture Date: Mon, 6 Nov 95 18:36:09 EST Lines: 18 Message-ID: <77631.jaeger@jupiter.ifn-magdeburg.de> X-NNTP-Posting-Host: dfnserv1.urz.uni-magdeburg.de X-Minuet-Version: Minuet1.0_Beta_14.7 X-Popmail-Charset: English subject: hippocampal slice culture I would like to culture hippocampal slices from adult rats. To my knowledge, nobody succeeded in doing this so far. I would like to get in touch with people who alredy attempted to culture hippocampal slices from adult rats or other animals. I would like to obtain information about the culture conditions succesfully or unsuccessfully tried (culture media, additives etc.) and I appreciate every helpful comment on this subject. You can respond directly or send me an e-mail. Sincerely yours Ulli Please mail to: schroede@jupiter.ifn-magdeburg.de e-mail: schroede@jupiter.ifn-magdeburg.de From owner-biophysics@net.bio.net Sun Nov 05 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!fdn.fr!jussieu.fr!univ-angers.fr!re.math-appli-uco.fr!lulu_ From: lulu_@re.math-appli-uco.fr (GIRARD Lucile) Newsgroups: bionet.biophysics Subject: Biomaterials Date: 6 Nov 1995 16:56:23 GMT Organization: Universite d'Angers, France Lines: 3 Distribution: world Message-ID: <47lenn$lql@news.univ-angers.fr> NNTP-Posting-Host: re.math-appli-uco.fr sixth November I am looking for any information about biomaterials, made out of plastics. Thank you From owner-biophysics@net.bio.net Mon Nov 06 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!sdd.hp.com!usc!chi-news.cic.net!simtel!harbinger.cc.monash.edu.au!news.rmit.EDU.AU!news.unimelb.EDU.AU!ucsvc.ucs.unimelb.edu.au!u2528082 From: u2528082@ucsvc.ucs.unimelb.edu.au Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.general,bionet.microbiology,bionet.molbio.proteins Subject: Re: biophysics vs. biochem speculatin'.... Date: 7 Nov 95 12:29:54 +1100 Organization: The University of Melbourne Lines: 48 Distribution: world Message-ID: <1995Nov7.122954@ucsvc.ucs.unimelb.edu.au> References: <47a8co$3s5@ixnews2.ix.netcom.com> NNTP-Posting-Host: hermes.ucs.unimelb.edu.au Xref: biosci bionet.biophysics:1403 bionet.cellbiol:3361 bionet.general:18322 bionet.microbiology:3868 bionet.molbio.proteins:6211 In article , "C. Greiner" writes: > > > On Thu, 2 Nov 1995, George Munson wrote: > >> > a) the **physical** arrangement of the peptide bonds - do the bonds and >> > the amino acids they're attached to form a smooth, rather than jagged >> > or discrete, surface? >> > >> > b) the tertiary or quarternary folding of the protein(s) in his fur - >> > ie, are the proteins physically arranged in some special way so that >> > they are experienced by us as being *soft*? >> > >> > c) the sequencing of the amino acids themselves, or >> > >> > d) some other phenomenom that I haven't thought of and probably can't >> > even imagine right now? >> >> Hmm. Okay this is my best guess and I chose "b." I doubt tactile >> perception is sensitive enough to distinguish peptide bonds or aa sequence >> per se. What you perceive is the much larger scale of the hair which is >> of course ultimately a result of c, a, and b. My guess is softness is >> related to rigidity or the lack thereof. Rigidity of the overall hair >> would likely arise from intermolecular disulfide cross linkages which >> would be tertiary/quartenary in nature. >> -- >> George Munson >> BMBCB, Northwestern University >> Evanston, IL USA >> george-munson@nwu.edu >> > > Although I agree (mostly) with George I wonder if the answer is not "d" > with the "softness" relating to the degree of secretion of oils onto the > hair rather than the actual molecular structure of the hair itself. Thus > the reason high price furs are often from water associated mammals (oil > serves as a water barrier) e.g. beaver, mink, fur seal, otter, etc... As all Australians know, the softness of wool is directly associated with fibre diameter. Wool with diameters over 20 microns is considered relativelycoarse and is itchy or scratchy on the skin. Diameters in the 12-14 micron range is considered superfine and is soft to the touch eg. cashmere. EM of these fibres show a relatively scaley appearance. To think that you can determine aa bonding by touch-wow. John Walker From owner-biophysics@net.bio.net Mon Nov 06 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!solaris.cc.vt.edu!usenet From: Leah Belinda Shaw Newsgroups: bionet.biophysics Subject: Where can I learn biophysics? Date: 7 Nov 1995 02:57:18 GMT Organization: Virginia Tech, Blacksburg, Virginia Lines: 12 Message-ID: <47mhuf$2nj@solaris.cc.vt.edu> NNTP-Posting-Host: asm4-1.sl104.cns.vt.edu Double majoring in physics and biology at Virginia Tech, I would like a better understanding of the relationship between these two fields. If I could study biophysics, I would (besides enjoying it!) find new uses for the information I am learning. However, my college does not offer a biophysics class. Can anyone recommend an introductory book on biophysics that a sophomore or junior would be able to comprehend? Or can someone convince my college of the importance of biophysics so that it might be persuaded to offer a course on the subject? (The latter is probably unlikely.) Thank you for any help you provide. Leah Shaw lshaw@vt.edu From owner-biophysics@net.bio.net Mon Nov 06 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!news.luc.edu!orion.it.luc.edu!scukier From: scukier@orion.it.luc.edu (Sam Cukierman) Newsgroups: bionet.biophysics Subject: Where can I learn biophysics? Date: 7 Nov 1995 16:30:48 GMT Organization: Loyola University Chicago Lines: 9 Message-ID: <47o1jo$2r1@artemis.it.luc.edu> NNTP-Posting-Host: 147.126.1.9 NNTP-Posting-User: scukier X-Newsreader: TIN [version 1.2 PL2] I find Biophysical Chemistry by P.R.Bergethon and E.R. Simons (Springer Verlag,1990, ISBN 0-387-97053-3) a nice introduction to Biophysics. Of course, it does not cover everything in Biophysics, but it is a good basic start. There are many biophysically oriented texts but a specific field of investigation must be identified first. Do you have any specific field of interest? If you let us know, we may provide you with a more specific reference. It is always encouraging to see students interested in biophysics. From owner-biophysics@net.bio.net Mon Nov 06 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!EU.net!uknet!bristol.st.com!ftel.co.uk!bham!usenet From: Gabriel Landini Newsgroups: bionet.biophysics Subject: NEW BOOK: FUTURE HEALTH Date: Tue, 07 Nov 1995 10:14:30 -0800 Organization: The University of Birmingham, UK. Lines: 24 Message-ID: <309FA206.A6@bham.ac.uk> NNTP-Posting-Host: dial1.bham.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b1 (Windows; I; 16bit) New book! Pickover, C. (1995) FUTURE HEALTH, Computers and Medicine in the Twenty-First Century. St. Martin's Press: New York. ISBN 0-312-12602-6. This collection considers the tremendous effects that computers will have on medicine and medical service in the next century. The book also gives a sampling of state-of-the-art application of computers in medicine. The chapters describe: o futuristic operating rooms o the challenges of future medical schools in preparing 21st-century physicians o futuristic fractal models in pathology o the use of new medical imaging technologies o the use of electronic gophers to obtain medical information o digital dentistry o the use of artificial intelligence in medical diagnosis o computer conferencing for medical consulting o bloodless robotic surgery o making solid models from medical images o futuristic examinations rooms and much more.... From owner-biophysics@net.bio.net Tue Nov 07 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!utcsri!utnut!torn!news.unb.ca!scratchy.mi.net!rogue From: rogue@mi.net (rogue) Newsgroups: bionet.biophysics Subject: What is It? Date: Tue, 07 Nov 95 21:10:01 GMT Organization: MIS Fredericton Lines: 4 Message-ID: <47ohv9$lk_003@rogue.mi.net> NNTP-Posting-Host: rogue.mi.net To: All X-Newsreader: News Xpress Version 1.0 Beta #3 Well, I'm particularly interested in Physics, and have always liked Biology, So what exsactly is biophysics?.. Thanks, it might be a possible career for me.. From owner-biophysics@net.bio.net Tue Nov 07 22:00:00 1995 Path: biosci!ONREUR.NAVY.MIL!ivodyanoy From: ivodyanoy@ONREUR.NAVY.MIL (Igor Vodyanoy) Newsgroups: bionet.biophysics Subject: International Science and Technology Cen Date: 8 Nov 1995 01:10:11 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 138 Sender: daemon@net.bio.net Distribution: world Message-ID: <1995Nov08.095900.1027.37558@smtp-gw.ehis.navy.mil> NNTP-Posting-Host: net.bio.net October 6, 1995 EUROPEAN BIOPHYSICS Igor Vodyanoy ____________________________________________________________ An Office Of Naval Research-European Office Biophysics Newsletter No 13 "International Science and Technology Center" ____________________________________________________________ WHAT IS THE ISTC? The International Science and Technology Center is an inter- governmental organization established by an international agreement. It develops, approves, finances, and monitors science and technology projects that address its objectives as set forth below. The initial Parties to the agreement are the European Union, Japan, the Russian Federation, and the United States of America. Recently, Finland, Sweden, and the Republic of Georgia acceded to the agreement. The initial program budget is about $70 million. The Center has three administrative bodies: The Governing Board consisting of representatives of the initial Parties; The international Scientific Advisory Committee (SAC); and The Secretariat, located in Moscow, which includes the Executive Director and three Deputy Directors. OBJECTIVES OF THE ISTC The main objectives are: 1) to provide weapons scientists and engineers in Russia and other interested states of the CIS opportunities to redirect their talents to peaceful activities; 2) to contribute thereby to the transition to market-based economies responsive to civil needs; 3) to support basic and applied research and technology development for peaceful purposes; and, 4) to promote the integration of scientists and engineers from Russia and other CIS states into the international scientific and engineering communities. To help achieve these objectives the Center assists in matching former weapons scientists and engineers from Russia and other CIS states with foreign colleagues who have similar interests in research and development for peaceful purposes. ELIGIBLE INSTITUTIONS AND USE OF ISTC FUNDS Project proposals can be submitted by any person, institution, government, or intergovernmental or non- governmental organization. A large share of the funds are used to provide financial support for weapon scientists and engineers from the CIS members. Smaller amount of funds are used to cover limited equipment costs and costs of international travel. Foreign collaboration in ISTC projects is strongly encouraged. However, ISTC funds are not intended to support foreign collaborators. DECISION-MAKING PROCESS Proposals are submitted to the Executive Director of the Center. Each proposal must comply with the ISTC INSTRUCTIONS FOR PROPOSAL PREPARATION. The Executive Director must also receive the approval of the state(s) in which the work is to be carried out before transmitting the proposal for further consideration. Each complete proposal, including the official approval, is transmitted to the Governing Board without delay. The Governing Board, taking into account the views of the SAC, makes decisions regarding projects on a consensus basis during its meetings which are scheduled no fewer than four times annually. Projects can be financed by one or several Parties. The total number of projects approved since the opening of the Center in March, 1994, is 55, representing a total contribution of approximately $31 million. The smallest project is $37,000 and the largest $3.1 million. The projects will sponsor more than 3,500 scientists and engineers for periods of up to three years. Projects have been approved in such fields as nuclear reactor safety, radioactive waste management, medicine, and laser technology. Several projects could provide direct support to developing technologies related to international efforts in verification of nuclear test ban treaties, disposal of weapons of mass destruction, and environmental monitoring HOW TO APPLY? Additional information may be obtained from the following address: International Science and Technology Center P O Box 25 Ulitsa Luganskaya, 9 Moscow, Russian Federation 115516 Tel-7-095-321-46-65 Fax-7-095-321-47-44 POINT OF CONTACT: Mr. James A. Luedeke International Science and Technology Center Finance Officer Tel: (7-095)321 48 65 Fax: (7-095)321 47 44 ___________________________________________________________ The Office of Naval Research, Europe is dedicated to providing current information on science and technology development in European countries. In order to better meet your needs, our Associate Directors and Scientists are very much interested in knowing what your specific science and technology interests are. Please provide feedback! Furthermore, if there are others who you are aware of that have interest in these newsletters, please pass their e-mail address to me and I will add them to the distribution list. ************************************************************ * for more information on this topic contact: * *Igor Vodyanoy, Ph.D. ONR Europe * *Associate Director, Biophysics 223 Old Marylebone Rd * *TEL: 44-171-514-4131 London, NW1 5TH * *FAX: 44-171-723-6359 England * *E-mail ivodyanoy@onreur.navy.mil * *Military address: ONREUR, PSC 802 Box 39, FPO AE 09499-0700* ************************************************************ From owner-biophysics@net.bio.net Tue Nov 07 22:00:00 1995 Path: biosci!RATREE.PSU.AC.TH!suchada From: suchada@RATREE.PSU.AC.TH (Suchada CHANTRAPROMMA) Newsgroups: bionet.biophysics Subject: unsubscribe Date: 7 Nov 1995 19:11:28 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net UNSUB Suchada From owner-biophysics@net.bio.net Tue Nov 07 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!spcuna!rutgers!csn!magnus.acs.ohio-state.edu!lerc.nasa.gov!purdue!mozo.cc.purdue.edu!aim.et.iupui.edu!NewsWatcher!user From: lipids@nowhere.com (Stillwell's Lab) Newsgroups: bionet.biophysics Subject: uncoupler anthelmintics Date: Wed, 08 Nov 1995 15:06:32 -0600 Organization: IUPUI Biology Lines: 7 Message-ID: NNTP-Posting-Host: 134.68.79.62 I am interested in information on anthelmintics that are uncouplers. I have already found info on benzimidazoles and salicyclanilides. I am looking for papers that show avermectins , imidazoles , or any other family of compounds that are uncouplers of oxidative phosphorylation. If you can help me please post here or email me at ijeg500@indyvax.iupui.edu thanks a lot jim From owner-biophysics@net.bio.net Wed Nov 08 22:00:00 1995 Path: biosci!neubio.sld.ar!Postmaster From: Postmaster@neubio.sld.ar (Administrador del Nodo) Newsgroups: bionet.biophysics Subject: What is it, biophysics? (Rogue's inquiry) Date: 9 Nov 1995 15:43:22 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 82 Sender: daemon@net.bio.net Distribution: world Message-ID: <923ki334@neubio.sld.ar> NNTP-Posting-Host: net.bio.net Dear Rogue, Biophysics is a disciplinary blending central for the forthcoming decades. You shall be able of earning your living and sharing a beautiful adventure in sciences and in industry by embracing it. I'm not prone to post he- re what you can find by yourself there, in any recent en- cyclopaedia; so let me end this answer hoping that you de- vote some time to study those books, solely mentioning in what follows a big and unusual point that you shall not find in those sources. Biophysics also studies how the soup, that does not think, after you get it inside your body does ma- ke your thoughts. Or, if you are troubled by thinking that your soul produces them, let me reformulate: Biophysics also studies how the soup, that does not think, when forming your brain makes the materials the brain offers to your soul for "reading". Viz., feelings, emotions, perceptual subjective not-structuralities (colours, odours and so on); that is all the contents of sensuality added upon reproduction- and nutrition-procuring behaviour by natural selection since prehistoric times. With more difficult words: neurobiology of the noematic contents is a freshly evolving part of biophysics. (Again: look for "noema" at any good dictionary. Be curious!) You cannot find the problems concerning this exciting process in usual biophysical texts, because Western culture put them in theology or psychology, not in biophysics. But during your life I bet you shall see a major breakthrough in this concern, because industries need machines able of turning accidents into opportunities ("Non-Turing automata") and religious doctrines might be able of relaxing its interest on what "is given to the soul for reading" (upon another major conceptual transformation in the humanities, rediscovering so- me old views, of what is a person, grounded in why you find yourself in your family rather than, instead, in the mine). Such industrial requirements and such doctrinary relaxation are making room for biophysical research on the differential -or specific- production of the diverse sensa- tions -independently of their being experienced, a concern still proper of metaphysics and not of natural sciences-. By the present, and specially in those most well-organized countries which thus needed strong social prefigurations, this important subject is by no means studied by biophysicists. But in other, less well-organized countries, paradoxically, a good corpus of notices has been gathered on it. Some researchers are also quite sure that some investigations are being conduced not in a public fashion, to produce said "non-Turing automata". (We here in our institution are compromised with making public everything we could scrap on this!). So this subject, truly in the front of advance of biophysics during your lifetime, may constitute a field to keep an eye on while you become engaged in the studies of more conventional curricula... also most beautiful and excit- ing in themselves! Be curious!! Mariela =*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*= Prof. Mariela Szirko, Centro de Investig. Neurobiologicas, Ministry of Health & Welfare, Argentine Republic; and Lab. of Electroneurobiological Res., Hospital "Dr. Jose Tiburcio Borda", Municipality of Buenos Aires, Office: Phone/Fax (54 1) 306 -7314 e-mail Standard disclaimer: Las opiniones de este mensaje son personales y no comprometen las dependencias a cargo de la firmante. Reply to THIS message, ONLY to: =*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*= From owner-biophysics@net.bio.net Wed Nov 08 22:00:00 1995 Newsgroups: bionet.biophysics Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!utcsri!cannon.ecf!azizi From: azizi@ecf.toronto.edu (AZIZI SOUFIANE) Subject: Re: Cut-oocyte voltage-clamp: help needed; X-Nntp-Posting-Host: skule.ecf Message-ID: Sender: news@ecf.toronto.edu (News Administrator) Organization: University of Toronto, Engineering Computing Facility References: <47qtfp$im_002@service.uci.edu> Date: Thu, 9 Nov 1995 21:12:13 GMT Lines: 30 In article <47qtfp$im_002@service.uci.edu>, Quoc Thang NGUYEN wrote: > Dear Netters, > >Is there anyone using a cut-oocyte voltage-clamp (developed by E. Stefani)? > >1) Size of the capacitance transient: I cannot go below 2ms with a 100mV pulse; >I reduced the resistance of the bridges as much as possible (~1.5kOhms), used >a vaseline rim, etc..., to no avail. >2)Stability of the preparation: the membrane resistance loses its value rapidly >within a few minutes. I use the saponin permeabilization method. > I am not using the COVG method; However, I read the original paper (Taglialatela et al. Biophys. J. 61:78-82. 1992). It seems like the speed of the clamp and stability of the preparation depend on the thickness of the vasiline rim between the membrane and the upper pool. The seal resistance (they claim) was increased by adding solution to the upper chamber (i.e. by raising the hydrostatic pressure on the upper vasiline rim. Have try to vary the seal thickness and the amount of solution in the upper chamber? Soufiane Azizi Electrical and Computer Engineering Dep. The University of Toronto. From owner-biophysics@net.bio.net Wed Nov 08 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!info.ucla.edu!csulb.edu!drivel.ics.uci.edu!news.service.uci.edu!rico-pc From: qnguyen@parker.bio.uci.edu (Quoc Thang NGUYEN) Newsgroups: bionet.neuroscience,bionet.biophysics Subject: Cut-oocyte voltage-clamp: help needed; Date: Wed, 08 Nov 95 18:38:49 GMT Organization: University of California Irvine Lines: 28 Message-ID: <47qtfp$im_002@service.uci.edu> NNTP-Posting-Host: rico-pc.bio.uci.edu X-Newsreader: News Xpress Version 1.0 Beta #3 Xref: biosci bionet.neuroscience:11147 bionet.biophysics:1410 Dear Netters, Is there anyone using a cut-oocyte voltage-clamp (developed by E. Stefani)? This device is supposed to clamp a small patch of an oocyte in "no time" (<<1ms). I am having trouble using a home-made design. Although everything seems to be working fine with a model cell (the clamp can follow a 100mV voltage-jump with a capacitive transient that settles within a few nA in 500us, with a filter setting at 2-5kHz; the capacitance of the membrane is 50nF), I am having trouble using it on the real thing. I have two main problems (not mentioning several minor ones!): 1) Size of the capacitance transient: I cannot go below 2ms with a 100mV pulse; I reduced the resistance of the bridges as much as possible (~1.5kOhms), used a vaseline rim, etc..., to no avail. 2) Stability of the preparation: the membrane resistance loses its value rapidly within a few minutes. I use the saponin permeabilization method. I would be very grateful if you can share your experience in using a cut-oocyte voltage clamp on this newsgroup. Thank you in advance. Quoc Thang NGUYEN Laboratory of Cellular and Molecular Neurobiology Dept. of Psychobiology University of California, Irvine Irvine, CA92717 USA Ph: (714) 824-4730 Fx: (714) 824-3522 From owner-biophysics@net.bio.net Thu Nov 09 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!info.ucla.edu!nnrp.info.ucla.edu!modem1.mbi.ucla.edu!steere From: steere@ewald.mbi.ucla.edu (Boyd Steere) Newsgroups: bionet.biophysics Subject: Reference sought for ion activities Date: Fri, 10 Nov 1995 00:54:44 Organization: University of California at Los Angeles Lines: 9 Distribution: world Message-ID: NNTP-Posting-Host: modem1.mbi.ucla.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Hello, all: I am seeking a good reference which contains tables of activity coefficients for salts which are common in biological systems and are measured in a variety of temperatures and concentrations. Where do you turn to find collected activity information in more detail than that provided by the CRC Handbook? Thanks From owner-biophysics@net.bio.net Thu Nov 09 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!sgigate.sgi.com!rutgers!oitnews.harvard.edu!purdue!lerc.nasa.gov!magnus.acs.ohio-state.edu!dopearso From: dopearso@magnus.acs.ohio-state.edu (Douglas C Pearson) Newsgroups: bionet.biophysics Subject: Re: What is It? Date: 10 Nov 1995 09:19:03 GMT Organization: The Ohio State University Lines: 33 Message-ID: <47v5e7$c4m@charm.magnus.acs.ohio-state.edu> References: <47ohv9$lk_003@rogue.mi.net> NNTP-Posting-Host: beauty.magnus.acs.ohio-state.edu In article <47ohv9$lk_003@rogue.mi.net>, rogue wrote: > Well, I'm particularly interested in Physics, and have always liked >Biology, So what exsactly is biophysics?.. Thanks, it might be a possible >career for me.. ARRRRRRGGGGGHHHHHH! says chuck at 4 AM while reading bionet.biophysics. to answer the two "what is biophysics?" questions, at least from my view point: whenever you use physical techniques to study biological systems, you're doing biophysics. this includes such basic techniques as exclusion chromatography or absorption spectroscopy, and takes off from there to all kinds of spectroscopy, light scattering, x-ray crystallography, osmotic pressure studies, modeling/ computational techniques (my personal favorite)...and i'm just talking about studying biological molecules! obviously, i could go on and on... to get more general, and to try to draw in some other of the regular posters here, we get this question on this group often enough that i wonder why we don't get some kind of FAQ file together for regular posting so that people who don't otherwise know might have a clue what biophysics is, especially since the question "what is biophysics?" would get ten different answers from ten different biophysicsts. i think i've mentioned this to somebody on this newsgroup before, but it's been long enough ago that my memory is failing. and, of course, i probably have as much time to take up this kind of a project as anybody else on tihs newsgroup. that is, not nearly enough. 8-) i say again, arrrrgggghhhh... chuck -- chuck pearson - dopearso@magnus.acs.ohio-state.edu osu biophysics program, chemistry 121 ta, daddy of amelia catherine pearson. From owner-biophysics@net.bio.net Fri Nov 10 22:00:00 1995 Path: biosci!rutgers!uwm.edu!chi-news.cic.net!newsfeed.internetmci.com!xmission!stellar.comnet.com!news.cc.utah.edu!news.cs.utah.edu!cc.usu.edu!davef From: davef@cc.usu.edu Newsgroups: bionet.neuroscience,bionet.biophysics Subject: Re: Cut-oocyte voltage-clamp: help needed; Message-ID: <1995Nov10.112640.66517@cc.usu.edu> Date: 10 Nov 95 11:26:40 MDT References: <47qtfp$im_002@service.uci.edu> Organization: Utah State University Lines: 30 Xref: biosci bionet.neuroscience:11175 bionet.biophysics:1415 I, too, wrestled with a cut-open oocyte (vaseline gap) clamp for several months, often frustrated by inability to clamp quickly or adequately. I think your problems may be due to inadequate clamping & isolation of the guard chamber. Leak between guard & upper chambers is tough to get rid of, and does weird things. Go *VERY LIGHTLY* with the vaseline. Put it on, then wipe it off with a finger so there is barely a fine film ... your seals should then improve between inside & guard chamber. Be careful your electrode (monitors inside voltage) doesn't push the membrane away from the underside of the guard chamber. You can run fine platinum wires through your agar bridges, for decreased resistance, if you want. If your membrane doesn't hold up, you may be leaving the saponin in too long. Go ahead and start rinsing it out before you notice the resistance to the inside (capacitance artifacts) change; sometimes the solution movement finishes the job, and if not, you can always go back and add more ...it's a lot easier than starting over from the outset with a new oocyte. You might want to check out my TEV clamping page... The artifacts and some of the problems with TEV are applicable to cut-open clamping too: http://cc.usu.edu/~davef/tev/tev.html Anyway, good luck. -dave From owner-biophysics@net.bio.net Sat Nov 11 22:00:00 1995 Path: biosci!rutgers!concert!gatech2!news.sprintlink.net!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!uknet!bhamcs!news.ox.ac.uk!news From: "Simon M. Brocklehurst" Newsgroups: bionet.biophysics Subject: ANNOUNCE: NAOMI Version 2.3 Date: Sun, 12 Nov 1995 18:49:48 +0000 Organization: Oxford University Lines: 67 Message-ID: <30A641CC.167EB0E7@bioch.ox.ac.uk> NNTP-Posting-Host: nmrv.ocms.ox.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b1N (X11; I; SunOS 4.1.3_U1 sun4m) NAOMI - Version Upgrade Announcement (Please note, NAOMI is provided at zero charge for academic use) (e-mail contact smb@bioch.ox.ac.uk) _____________________________________________________________________________ The computer program NAOMI Version 2.3 is available as of now from the NAOMI Web site at: http://www.ocms.ox.ac.uk/~smb/Software/N_details/naomi.html or via anonymous ftp from: nmrz.ocms.ox.ac.uk in directory pub/smb/naomi/ Users of versions older than 2.10 will need new license keys to allow the upgrade to work (please contact the author in this case). _____________________________________________________________________________ Upgrade features : machine-parsable output for interface to bioinformatics software, main-chain modelling, general residue selection, new solvent accessibility commands, additions to NMR structure refinement module. Fixes of minor bugs in pdb output and molscript output commands. _____________________________________________________________________________ What is NAOMI? NAOMI is an easy-to-use, state-of-the-art computer program which is aimed at both specialist and non-specialist researchers who make use of three-dimensional structures of proteins in their work. It has hundreds of users Worldwide. Some facilities offered by the program for working with structure include: automatic 'key' residue identification automatic hydrophobic core/packing analysis automatic hydrogen bonds main-chain and side-chain identification (including high quality energy calculations) automatic secondary structure (helix, strand and turn) classification using fuzzy logic automatic supersecondary structure classification (beta-hairpin loops) conformational parameters: phi,psi,chi1,chi2,chi3,chi4,chi5 etc solvent accessibility (both absolute and percentage) calculations automatic identification of disulphide bonds, salt bridges, chain-breaks side-chain modelling and manipulation applying symmetry operators automatic structure repair (building in missing atoms) NMR structure refinement module interfaces to graphics programs (MOLSCRIPT (and thus Raster3D), INSIGHT, QUANTA to allow automatic preparation of figures More details are available on the Web site. NB NAOMI currently works only on Silicon Graphics workstations running IRIX 5.* _____________________________________________________________________________ | | ,_ o Simon M. Brocklehurst, | / //\, Oxford Centre for Molecular Sciences, Department of Biochemistry, | \>> | University of Oxford, Oxford, UK. | \\, E-mail: smb@bioch.ox.ac.uk | WWW: http://www.ocms.ox.ac.uk/~smb/ |____________________________________________________________________________ From owner-biophysics@net.bio.net Sat Nov 11 22:00:00 1995 Path: biosci!neubio.sld.ar!Postmaster From: Postmaster@neubio.sld.ar (Administrador del Nodo) Newsgroups: bionet.biophysics Subject: Humour & Neural Networks Date: 12 Nov 1995 12:25:21 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 79 Sender: daemon@net.bio.net Distribution: world Message-ID: <303kl394@neubio.sld.ar> NNTP-Posting-Host: net.bio.net >From Postmaster Sun, 12 Nov 95 16:43:23 ARG remote from neubio >Received: by neubio.sld.ar (UUPC/PcCorreo 3.0) with UUCP; Sun, 12 Nov 95 16:43:23 ARG >Date: Sun, 12 Nov 95 16:43:23 ARG >From: Administrador del Nodo >Message-ID: <221kl197@neubio.sld.ar> >X-Mailer: UUPC/PcCorreo 3.0 >To: neuroscience@net.bio.net >Subject: Humour & Neural Networks > >Hello again, friend netters! > > Philip Dorrell (p@dorrell.demon.co.uk) >comments on my former posting: > >> Lack of empathy seems to me to be distinct to lack of >>sense of humour. Maybe with sufficient damage both could >>be obliterated, but that would only confuse the issue. >>Just because someone doesn't get a joke I don't see why >>they shouldn't exhibit concern about people. What I would >>expect is that people that lacked empathy wouldn't laugh >>at jokes involving anti-empathy, i.e. where something um- >>pleasant happens to someone. > > Philip's answer go deep! ("cala en honduras", >I would say in Spanish). If laugh is to be depicted by neural >networking, it ought to be like an Aplysia's beautifully coordi- >nated reflex. We even do not still know if birdsong is, or if >it is not, accompanied by empathy. But laughing (except when >sardonic :-)!) is humourous -or, it humours a joke- only if em- >pathizing with the situation understood as jocular. We here in >our Lab follow the analysis of Prof. Marcos Victoria ("La Risa") >and of Dr. Jose Ingenieros; but abroad you can find similar appre- >ciations in the line of thought of Dilthey's distinction between >explanation and understanding and, on the other hand, Bergson's >theses on "Le rire". Agreed: empathy and sense of humour are >pretty different, but you cannot have sense of humour if you lack >the sense of understanding the situations affecting your neighbours, >or "understanding" in the Diltheyan-Jasperian acception. And this >requires hylozoism. > > What is at the stake is the possibility for neural >networks, or in general for any kinetized, exogenist physics, to >depict laughing. > > Any non-hylozoist neuroscience framed in such a >physics can solely depict laughing by previous reducing it into a >reflex lacking empathy, i.e. an effect of parahippocampal electro- >stimulation. But if your electrostimulated subject tells you that >she feels merryment, then you ought to relinquish said reduction >and recognize hylozoistically that reflexes put in action other >processes differentially producing one-witness physical realities. > > If you preposit a dualism, of course you can insert >a hormic agency navigating accross the bushes of the neuroanatomical >structures, but only while resigning any physical research. > > Fortunately the cult bussiness now can thrive without >such hormic additions, so I hope in the next decades both physics >and the neurosciences based in it shall accompany the hylozoist stan- >ce of our remote and (until recently) confined tradition. > > Cyberkissing everybody! > Mariela =*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*= Prof. Mariela Szirko, Centro de Investig. Neurobiologicas, Ministry of Health & Welfare, Argentine Republic; and Lab. of Electroneurobiological Res., Hospital "Dr. Jose Tiburcio Borda", Municipality of Buenos Aires, Office: Phone/Fax (54 1) 306 -7314 e-mail Standard disclaimer: Las opiniones de este mensaje son personales y no comprometen las dependencias a cargo de la firmante. Reply to THIS message, ONLY to: =*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*= From owner-biophysics@net.bio.net Sun Nov 12 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!chi-news.cic.net!news.math.psu.edu!news.cac.psu.edu!newsserver.jvnc.net!princeton!cnn.Princeton.EDU!udel!news.udel.edu!che.udel.edu!asthagir From: Dilipkuma Asthagiri Newsgroups: bionet.biophysics Subject: Charge density Date: Sun, 12 Nov 1995 20:19:18 -0500 Organization: University of Delaware Lines: 9 Message-ID: NNTP-Posting-Host: che.udel.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Hi, I am studying (a simulation) adsorption of lysozyme Dynamax 300A-AX anion exchange column. Is there some estimate of the surface charge density for this (column) material? Even a crude estimate will be very helpful. Thanks in advance ... Dilip From owner-biophysics@net.bio.net Mon Nov 13 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!newsfeed.ACO.net!fstgal00.tu-graz.ac.at!balu.kfunigraz.ac.at!usenet From: "U.E. Winkler" Newsgroups: bionet.biophysics Subject: Particleproduction Date: 14 Nov 1995 11:38:43 GMT Organization: Inst. Theoret. Physics Lines: 18 Message-ID: <489v43$357@balu.kfunigraz.ac.at> NNTP-Posting-Host: caesar.kfunigraz.ac.at Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1S (X11; I; IRIX 5.3 IP22) X-URL: news:bionet.biophysics A friend of mine has an urgent problem and would be pleased if somebody could answer at least to one of the following questions (when I'm wrong in this newsgroup, please let me know if there exists a more suited one): o) In what way is it possible to produce spherical particles of size 10 micron, made of crystallized calcium phospate and/or glass (silica)? Maybe also: is it possible to obtain them from anywhere? o) Are there alternative methods to the "sol-gel" resp. "spherilizing" process? Thank you in advance, U. Winkler email uew@publix.kfunigraz.ac.at From owner-biophysics@net.bio.net Mon Nov 13 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sesqui.net!oitnews.harvard.edu!fas-news.harvard.edu!slippp2-08.fas.harvard.edu!user From: dan@rhino.harvard.edu (Dan Crevier) Newsgroups: bionet.biophysics Subject: Re: What is It? Date: Sat, 11 Nov 1995 13:11:46 -0500 Organization: Harvard University Lines: 22 Message-ID: References: <47ohv9$lk_003@rogue.mi.net> <47v5e7$c4m@charm.magnus.acs.ohio-state.edu> NNTP-Posting-Host: slippp2-08.fas.harvard.edu X-Newsreader: Yet Another NewsWatcher 2.0.6b4 In article <47v5e7$c4m@charm.magnus.acs.ohio-state.edu>, dopearso@magnus.acs.ohio-state.edu (Douglas C Pearson) wrote: > In article <47ohv9$lk_003@rogue.mi.net>, rogue wrote: > > Well, I'm particularly interested in Physics, and have always liked > >Biology, So what exsactly is biophysics?.. Thanks, it might be a possible > >career for me.. From the Harvard Biophysics T-Shirt: Biophysicist: 1. one who uses the principles of and techniques of physics to study biological phenomena 2. a biologist who knows enough physics to fix his or her own stereo 3. a physicist with the ill-conceived notion that there are more jobs in biology Dan -- ----- Dan Crevier : dan@rhino.harvard.edu : http://rhino.harvard.edu/dan/home.html From owner-biophysics@net.bio.net Mon Nov 13 22:00:00 1995 Path: biosci!LEX.LCCC.EDU!rcb1 From: rcb1@LEX.LCCC.EDU (Ron Blue) Newsgroups: bionet.biophysics Subject: Re: Particleproduction Date: 14 Nov 1995 12:32:57 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <489v43$357@balu.kfunigraz.ac.at> NNTP-Posting-Host: net.bio.net On 14 Nov 1995, U.E. Winkler wrote: > A friend of mine has an urgent problem and would be pleased > if somebody could answer at least to one of the following > questions (when I'm wrong in this newsgroup, please let me > know if there exists a more suited one): > > o) In what way is it possible to produce spherical > particles of size 10 micron, made of crystallized > calcium phospate and/or glass (silica)? > Maybe also: is it possible to obtain them from > anywhere? This is not my field! But I read an article (source unknown) some time in the last three months that describe how a tube of glass can be made to make matter flow like a wave with lasers electromagnetic fields. This suggest to me the possibility of making your 10 micron calcium phospate which will hopely crystallize during transport. Ron Blue > > o) Are there alternative methods to the "sol-gel" resp. > "spherilizing" process? > > Thank you in advance, > U. Winkler > email uew@publix.kfunigraz.ac.at > From owner-biophysics@net.bio.net Mon Nov 13 22:00:00 1995 Path: biosci!Gems.VCU.EDU!LSATIN From: LSATIN@Gems.VCU.EDU Newsgroups: bionet.biophysics Subject: question about position announcements Date: 14 Nov 1995 07:32:20 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HXMO7C6B2G9BZKQ2@Gems.VCU.EDU> Is it now not permitted to post openings in Biophysics on the Biophys bulletin board? I sent one out yesterday but it did not appear, suggesting a bulletin board manager snagged it, right? It seems more worthwhile given the current job market to allow this pretty legitimate use of this service vs some of the crud that has come down the line of late! Thanks for your input. Dr LS Satin From owner-biophysics@net.bio.net Mon Nov 13 22:00:00 1995 Path: biosci!rutgers!csn!carbon!night.primate.wisc.edu!newsspool.doit.wisc.edu!uwm.edu!psuvax1!news.eecs.nwu.edu!newsfeed.acns.nwu.edu!news.acns.nwu.edu!lucky147.acns.nwu.edu!user From: t-shinbrot@nwu.edu (Troy Shinbrot) Newsgroups: bionet.biophysics Subject: Re: Particleproduction Date: Tue, 14 Nov 1995 16:08:22 -0500 Organization: Northwestern University Lines: 27 Message-ID: References: <489v43$357@balu.kfunigraz.ac.at> NNTP-Posting-Host: lucky147.acns.nwu.edu X-Newsreader: Yet Another NewsWatcher 2.0.3b0 In article <489v43$357@balu.kfunigraz.ac.at>, "U.E. Winkler" wrote: > A friend of mine has an urgent problem and would be pleased > if somebody could answer at least to one of the following > questions (when I'm wrong in this newsgroup, please let me > know if there exists a more suited one): > > o) In what way is it possible to produce spherical > particles of size 10 micron, made of crystallized > calcium phospate and/or glass (silica)? > Maybe also: is it possible to obtain them from > anywhere? > > o) Are there alternative methods to the "sol-gel" resp. > "spherilizing" process? > > Thank you in advance, > U. Winkler > email uew@publix.kfunigraz.ac.at You might want to try Duke Scientific, which manufactures various kinds of microspheres. I don't know about calcium phosphate, but it's a place to start. -Troy Shinbrot Northwestern University From owner-biophysics@net.bio.net Tue Nov 14 22:00:00 1995 Path: biosci!BIOSCI.CBS.UMN.EDU!cgross From: cgross@BIOSCI.CBS.UMN.EDU ("Carol Gross") Newsgroups: bionet.biophysics Subject: Broadcast Announcement Date: 15 Nov 1995 09:22:59 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 42 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511151719.LAA19094@biosci.cbs.umn.edu> Reply-To: "Carol Gross" NNTP-Posting-Host: net.bio.net THE SECOND INTERNATIONAL SYMPOSIUM ON REVERSIBLE ASSOCIATIONS IN STRUCTURAL AND MOLECULAR BIOLOGY February 13-16, 1996, Bethesda, MD In February, 1994, an international conference centered on the topic of Reversible Associations in Structural and Molecular Biology (RASMB) was held at the University of Melbourne, Victoria, Australia. The meeting was widely held by its participants to have been one of the best of its kind in their experience, and there was essentially unanimous agreement that the Melbourne meeting should represent the first of a biannual series of symposia on this general topic. The Second International Symposium will focus on the central role of reversible associations in a wide variety of biophysical and biochemical phenomena. System-oriented sessions will be devoted to assembly of protein complexes, protein folding, protein-surface interactions, ligand-receptor interactions, protein-nucleic acid interactions, and MHC-T cell interactions. Method-oriented sessions will be devoted to solution methods, surface methods, structural methods, and theoretical and computational methods. On behalf of the organizing committee, I invite you to attend, present a poster, and contribute to the exchange of information in this vital and exciting field. A conference brochure and registration form may be obtained from Sylvia Delong, Conference Coordinator, FAES, One Cloister Court, Suite 230, Bethesda, MD 20814. Telephone: (301) 496- 7975, FAX: (301) 402-0174. NOTE: Due to the capacity of the Lister Hill Auditorium, registration will be limited to the first 120 respondents in order of postmark or fax date. Allen Minton minton@helix.nih.gov From owner-biophysics@net.bio.net Tue Nov 14 22:00:00 1995 Path: biosci!ICT.SLD.CU!NORMANDO From: NORMANDO@ICT.SLD.CU (Normando Iznaga) Newsgroups: bionet.biophysics Subject: (none) Date: 15 Nov 1995 10:06:15 -0800 Organization: Centro de Inmunologia Molecular Lines: 25 Sender: daemon@net.bio.net Distribution: world Message-ID: <9511141848.AA25840@infomed> NNTP-Posting-Host: net.bio.net Hi netters, I would like to know the e-mail of R. A. Thompson Editor in Chief of the Clinical and Experimental Immunology journal edited in Birmingham. Any kind of help will be gretafull. Thanks in advance Best wishes, !*************************************! ! M. Sc. Normando Iznaga Escobar ! ! Center of Molecular Immunology ! ! Division of Antibody Engineering ! ! PO BOX 16040, Havana, 11 600, Cuba ! ! ! ! Fax Number : (53 7) 33 50 49 ! ! (53 7) 33 35 09 ! ! Phone : (53 7) 21 68 11 ! ! (53 7) 21 79 33 ! ! e-mail : normando@ict.sld.cu ! !*************************************! From owner-biophysics@net.bio.net Tue Nov 14 22:00:00 1995 Path: biosci!ICT.SLD.CU!NORMANDO From: NORMANDO@ICT.SLD.CU (Normando Iznaga) Newsgroups: bionet.biophysics Subject: (none) Date: 15 Nov 1995 10:07:26 -0800 Organization: Centro de Inmunologia Molecular Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: <9511141849.AA25873@infomed> NNTP-Posting-Host: net.bio.net Hi netters, I would like to know the e-mail of W. James Irvine Editor in Chief of the Journal of Clinical and Laboratory Immunology edited in Edinburgh. Any kind of help will be greatfull. Thanks in advance Best wishes, !*************************************! ! M. Sc. Normando Iznaga Escobar ! ! Center of Molecular Immunology ! ! Division of Antibody Engineering ! ! PO BOX 16040, Havana, 11 600, Cuba ! ! ! ! Fax Number : (53 7) 33 50 49 ! ! (53 7) 33 35 09 ! ! Phone : (53 7) 21 68 11 ! ! (53 7) 21 79 33 ! ! e-mail : normando@ict.sld.cu ! !*************************************! From owner-biophysics@net.bio.net Tue Nov 14 22:00:00 1995 Path: biosci!rutgers!concert!gatech2!wa4mei!gatech!newsfeed.internetmci.com!newsxfer.itd.umich.edu!news.eecs.umich.edu!pravda.aa.msen.com!simtel!swidir.switch.ch!scsing.switch.ch!news.belwue.de!News.Uni-Marburg.DE!news.th-darmstadt.de!faui0n.informatik.uni-erlangen.de!uni-erlangen.de!winx03!wpxx02.toxi.uni-wuerzburg.de!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.biophysics Subject: Re: question about position announcements Date: 15 Nov 1995 13:13:04 GMT Organization: Dept. of Pharmacology, U Wuerzburg Lines: 19 Distribution: world Message-ID: <48cp10$qsc@winx03.informatik.uni-wuerzburg.de> References: <01HXMO7C6B2G9BZKQ2@Gems.VCU.EDU> NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] LSATIN@Gems.VCU.EDU wrote: > Is it now not permitted to post openings in Biophysics on the Biophys > bulletin board? I sent one out yesterday but it did not appear, > suggesting a bulletin board manager snagged it, right? As far as I know bionet.biophysics is not moderated. In addition, if a moderator rejects your post, he usually sends you an explanation by email. I would guess your news server just screwed up. Try again. (However, I still think it would be more sensible to post job offerings to bionet.jobs.offered, or whatever the name of this group is.) --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-biophysics@net.bio.net Tue Nov 14 22:00:00 1995 Path: biosci!rutgers!concert!news-server.ncren.net!decwrl!lll-winken.llnl.gov!usenet From: Chris Barry Newsgroups: bionet.biophysics Subject: Re: Protein-based computers Date: 15 Nov 1995 21:50:12 GMT Organization: Lawrence Livermore National Laboratory Lines: 2 Message-ID: <48dnak$pi5@lll-winken.llnl.gov> References: <48c4gt$b56@pulp.ucs.ualberta.ca> NNTP-Posting-Host: mackiller.llnl.gov Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; Linux 1.1.72 i486) X-URL: news:48c4gt$b56@pulp.ucs.ualberta.ca Most likely a PhD in computer engineering/physics/and biochemisty. Good luck From owner-biophysics@net.bio.net Tue Nov 14 22:00:00 1995 Path: biosci!rutgers!uwm.edu!newsspool.doit.wisc.edu!decwrl!tribune.usask.ca!rover.ucs.ualberta.ca!gpu2!scocquyt From: scocquyt@gpu2.srv.ualberta.ca (Dutch) Newsgroups: bionet.biophysics Subject: Protein-based computers Date: 15 Nov 1995 07:23:09 GMT Organization: University of Alberta Lines: 11 Message-ID: <48c4gt$b56@pulp.ucs.ualberta.ca> NNTP-Posting-Host: gpu2.srv.ualberta.ca X-Newsreader: TIN [version 1.2 PL2] Hi, I believe this question is relevant to this group: I am interested in the "field" of protein-based computers (for example, the use of bacteriorhodopsin for data storage) and I was wondering whether or not this is a biophysical field and if so what kind of academic background/degree is required in order to perform research in this field? Thanks for your help, Dutch -- From owner-biophysics@net.bio.net Wed Nov 15 22:00:00 1995 Path: biosci!BUHO.DPI.UDEC.CL!mbunster From: mbunster@BUHO.DPI.UDEC.CL (Marta Bunster B) Newsgroups: bionet.biophysics Subject: (none) Date: 16 Nov 1995 04:25:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <9511161515.AA20849@buho.dpi.udec.cl> NNTP-Posting-Host: net.bio.net unsubscribe From owner-biophysics@net.bio.net Wed Nov 15 22:00:00 1995 Path: biosci!ICT.SLD.CU!NORMANDO From: NORMANDO@ICT.SLD.CU (Normando Iznaga) Newsgroups: bionet.biophysics Subject: Re: (none) Date: 16 Nov 1995 07:44:51 -0800 Organization: Centro de Inmunologia Molecular Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: <9511152203.AA07728@infomed> NNTP-Posting-Host: net.bio.net Hi netters, I would like to know the e-mail of R. A. Thompson Editor in Chief of the Clinical and Experimental Immunology journal edited in Birmingham. Any kind of help will be greatfull. Thanks in advance Best wishes, !*************************************! ! M. Sc. Normando Iznaga Escobar ! ! Center of Molecular Immunology ! ! Division of Antibody Engineering ! ! PO BOX 16040, Havana, 11 600, Cuba ! ! ! ! Fax Number : (53 7) 33 50 49 ! ! (53 7) 33 35 09 ! ! Phone : (53 7) 21 68 11 ! ! (53 7) 21 79 33 ! ! e-mail : normando@ict.sld.cu ! !*************************************! From owner-biophysics@net.bio.net Wed Nov 15 22:00:00 1995 Path: biosci!Gems.VCU.EDU!LSATIN From: LSATIN@Gems.VCU.EDU Newsgroups: bionet.biophysics Subject: position available Date: 16 Nov 1995 06:15:30 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HXPENO7XJW9BZGXH@Gems.VCU.EDU> NNTP-Posting-Host: net.bio.net A postdoctoral position is available now in my laboratory for a qualified MD or PhD to study ion channels in pancreatic islet B-cells and their role in insulin secretion in normal and diabetic animals. Techniques include patch clamping, mathematical modelling, Ca-measurements with FURA including confocal microscopy, and channel expression in xeonpus oocytes. See Satin et al (Oct issue, Endocrinology), Kinard and Satin (Dec issue of Diabetes), Satin et al (Endocrine, Aug 1994), Satin et al (Biophys J., Jan 1994) for some publications. If you or someone you know of is interested in this position, please send CV and statement of research background to: LS Satin Departments of Pharmacology and Toxicology and Physiology Medical College of Virginia School of Medicine Virginia Commonwealth University Box 980524 Richmond, VA 23298-0524 phone (804) 828-7823 fax (804) 828-1532 FAX or email are also ok. From owner-biophysics@net.bio.net Wed Nov 15 22:00:00 1995 Path: biosci!daresbury!not-for-mail From: son of pps Newsgroups: bionet.biophysics Subject: WEB Course on Protein Structure Date: 16 Nov 1995 13:02:32 -0000 Lines: 17 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <48fcp8$3lq@mserv1.dl.ac.uk> X-Sender: pps2@iona.cryst.bbk.ac.uk Original-To: biophys@dl.ac.uk B i r k b e c k C o l l e g e Crystallography Department INTERNET COURSE IN PRINCIPLES OF PROTEIN STRUCTURE http://www.cryst.bbk.ac.uk/PPS2/ We are running a part-time London University Advanced Certificate course in Principles of Protein Structure on the Internet (a formalised version of last year's course). Units are: 1. IT and Bioinformatics 2. Protein Structure 3. Dissertation: topics in structure, function and dynamics Course runs for 1 academic year (3 terms) from 15 January to 25 October 1996. For details of course contents, administration and registration, see our Web pages at URL given above. Costs: 186 pounds sterling (EU) 500 pounds sterling (world) email: j.mcgill@mail.cryst.bbk.ac.uk From owner-biophysics@net.bio.net Thu Nov 16 22:00:00 1995 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.biophysics Subject: IMPORTANT: BIOSCI miniFAQ Date: 17 Nov 1995 02:00:42 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 196 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511171000.CAA12807@net.bio.net> NNTP-Posting-Host: net.bio.net This is a new "miniFAQ" designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) What to do about "spams," i.e., junk mail, ads, etc. 2) Examples of subscribing and unsubscribing to the mailing lists. 3) How to access BIOSCI/bionet newsgroup archives. 4) The BIOSCI user address and research interest directory. 1) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups) and mailing lists. The same postings are distributed on both media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the newsgroups from about 95% of the spams that are being sent to date. This means that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings. Unfortunately there are easy ways for determined spammers to override the moderation mechanism. We are working on new systems to provide access to our newsgroups over the WWW. These should be available soon, probably November 1995, and will allow you to use your Web browser to look at the news postings. While this will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. 2) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 3) How to access BIOSCI/bionet newsgroup archives. -------------------------------------------------- Back postings of all BIOSCI/bionet newsgroups can be found on the World Wide Web at URL http://www.bio.net/. There are several searchable newsgroup indices at this site. E-mail users can search the BIOSCI archives by using our waismail e-mail server. For instructions send the message help to waismail@net.bio.net. Leave the Subject: line blank (anything entered on the Subject: line is ignored). 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-biophysics@net.bio.net Thu Nov 16 22:00:00 1995 Path: biosci!rutgers!uwm.edu!omnifest.uwm.edu!omnifest.uwm.edu!not-for-mail From: marjorie@omnifest.uwm.edu (Marjorie A. Lundquist) Newsgroups: bionet.biophysics Subject: Biological Energy Date: 17 Nov 1995 14:45:09 -0600 Organization: Omnifest Lines: 9 Distribution: na Message-ID: <48is8m$1ge@omnifest.uwm.edu> NNTP-Posting-Host: 129.89.70.58 I wanted to respond to the person who was searching for others interested in biological energy, but I didn't manage a timely reply, so don't have an address. Would that person post again, or contact me directly, please? Thanks -- Marjorie ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Marjorie Lundquist P. O. Box 11831 Milwaukee, WI 53211-0831 USA marjorie@omnifest.uwm.edu ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ From owner-biophysics@net.bio.net Thu Nov 16 22:00:00 1995 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!enews.sgi.com!decwrl!tribune.usask.ca!skyfox.usask.ca!yangj From: yangj@skyfox.usask.ca Newsgroups: bionet.biophysics Subject: help: packing energy Date: 17 NOV 95 20:12:14 GMT Organization: University of Saskatchewan Lines: 13 Message-ID: <17NOV95.20121453@skyfox.usask.ca> NNTP-Posting-Host: sask.usask.ca Hello, everyone, I have a problem that I need your help. Could anybody tell me which program can calculate the packing energy between a domain of a molecule with symmetry-related molecules with pdb coordinates? I thank you in advance for your help. Sincerely, Jian Yang Department of Chemistry University of Saskatchewan Saskatoon, Saskatchewan Canada S7N 5C9 Tel: (306) 966-4366 Email: yangj@sask.usask.ca From owner-biophysics@net.bio.net Thu Nov 16 22:00:00 1995 Path: biosci!rutgers!uwm.edu!spool.mu.edu!agate!dog.ee.lbl.gov!news.cs.utah.edu!swen.emba.uvm.edu!newsmaster From: guilford@salus.med.uvm.edu (William H. Guilford) Newsgroups: bionet.biophysics Subject: Re: Particleproduction Date: Wed, 15 Nov 1995 16:24:53 GMT Organization: EMBA Computer Facility, The University of Vermont Lines: 23 Message-ID: <48d49p$kam@swen.emba.uvm.edu> References: <489v43$357@balu.kfunigraz.ac.at> NNTP-Posting-Host: 132.198.80.81 X-Newsreader: Forte Free Agent 1.0.82 "U.E. Winkler" wrote: > o) In what way is it possible to produce spherical > particles of size 10 micron, made of crystallized > calcium phospate and/or glass (silica)? > Maybe also: is it possible to obtain them from > anywhere? Silica glass beads can be obtained from Bangs. Sorry, I don't have an address handy. > o) Are there alternative methods to the "sol-gel" resp. > "spherilizing" process? You could try melting the glass with a blow torch. Catch the small particles that fly out (if you get any). Those will be small glass spheres that form by surface tension and harden while falling through the air. Catch them in something... ... or order them from Bangs, whichever is easier. ;) - Bill From owner-biophysics@net.bio.net Thu Nov 16 22:00:00 1995 Newsgroups: bionet.molec-model,bionet.biophysics Path: biosci!rutgers!uwm.edu!spool.mu.edu!torn!news.unb.ca!coranto.ucs.mun.ca!cs.mun.ca!andrewf From: andrewf@cs.mun.ca (Andrew Foster) Subject: literature on protein folding models? Message-ID: <1995Nov16.211834.8566@cs.mun.ca> Sender: usenet@cs.mun.ca (NNTP server account) Organization: CS Dept., Memorial University of Newfoundland X-Newsreader: NN version 6.4.19 Date: Thu, 16 Nov 1995 21:18:34 GMT Lines: 23 Xref: biosci bionet.molec-model:666 bionet.biophysics:1435 Hello. After an absence of a few years, I am continuing some research on the protein folding problem. That is, I am refining a mathematical model to predict a globular protein's tertiary structure from its primary sequence. But I'm a little behind on the literature. Can anyone point me to any recent (last 5 years) published developments in the field? Journal review articles would be ideal. To be more specific, I am mainly interested in semi-empirical methods that try to exploit general geometric characteristics of known folded proteins. Some of these are known as distance geometry models. I am not as interested in homologous modelling or secondary structures. Thank you. -- Dr. Andy Foster e-mail: andrewf@math.mun.ca Dept of Mathematics and Statistics Memorial University of Newfoundland Phone : (709) 737-8096 St. John's, NFLD, Canada A1C 5S7 Fax : (709) 737-3010 From owner-biophysics@net.bio.net Fri Nov 17 22:00:00 1995 Path: biosci!rutgers!concert!gatech2!swrinde!newsfeed.internetmci.com!info.ucla.edu!ucsbuxb.ucsb.edu!ucsbuxb!kohlstaedt From: kohlstaedt@sbmm1.ucsb.edu (Lori Kohlstaedt) Newsgroups: bionet.biophysics Subject: Re: Where can I learn biophysics? Date: Sat, 18 Nov 95 05:26:15 GMT Organization: UCSB Chem Lines: 15 Message-ID: NNTP-Posting-Host: 128.111.114.141 X-Newsreader: VersaTerm Link v1.1.1 Leah, If you are interested in the version of biophysics that is concerned with how and why macromolecules interact with themselves, each other, and the environment then I suggest T.E. Creighton's "Proteins: Structures and Molecular properties." It's a good introduction and actually covers a bit about nucleic acids and mambranes as well. I use it for a graduate course I teach. *************************************** Lori Kohlstaedt Dept. of Chemistry, University of California, Santa Barbara Santa Barbara, CA 93106 (805) 893-7040 *************************************** From owner-biophysics@net.bio.net Fri Nov 17 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!newsfeed.internetmci.com!newsxfer.itd.umich.edu!news.itd.umich.edu!usenet From: bhughes@umich.edu (Bret Hughes) Newsgroups: bionet.biophysics Subject: Re: Reference sought for ion activities Date: 18 Nov 1995 14:53:33 GMT Organization: University of Michigan Lines: 21 Distribution: world Message-ID: <48ks1d$30s@lastactionhero.rs.itd.umich.edu> References: NNTP-Posting-Host: host-142.subnet-80.med.umich.edu Mime-Version: 1.0 X-Newsreader: WinVN 0.93.14 You can probably find what you are looking for in: "Electrolyte Solutions" by R.A. Robinson and R.H. Stokes, 2nd Edition, 1959; Butterworths, London. Unfortunately, this great resource is out of print. You may be able to find a copy at your institution's library. In article , steere@ewald.mbi.ucla.edu says... > >Hello, all: > >I am seeking a good reference which contains tables of activity coefficients >for salts which are common in biological systems and are measured in >a variety of temperatures and concentrations. Where do you turn to find >collected activity information in more detail than that provided by the CRC >Handbook? > >Thanks From owner-biophysics@net.bio.net Fri Nov 17 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!news1.ucsd.edu!usenet From: Kevin Shreder Newsgroups: bionet.biophysics Subject: Re: Where can I learn biophysics? Date: 18 Nov 1995 23:26:54 GMT Organization: UCSD Lines: 6 Message-ID: <48lq3u$rj@news2.ucsd.edu> References: NNTP-Posting-Host: chegood10.ucsd.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:kohlstaedt.1167060015A@ucsbuxb.ucsb.edu A great three part book series is called Biophysical Chemistry by Cantor and Schimmel. The series covers a broad range of topics in both straightforward simplicity and great detail. Most science libraries should have this series. From owner-biophysics@net.bio.net Sat Nov 18 22:00:00 1995 Path: biosci!agate!spool.mu.edu!uwm.edu!vixen.cso.uiuc.edu!sdd.hp.com!col.hp.com!csn!carbon!hermes.cair.du.edu!usenet From: dbovee@phoebe.cair.du.edu (David Bovee) Newsgroups: bionet.molec-model,bionet.biophysics Subject: Re: literature on protein folding models? Date: 19 Nov 1995 05:43:19 GMT Organization: University of Denver Lines: 33 Message-ID: <48mg5n$c7a@hermes.cair.du.edu> References: <1995Nov16.211834.8566@cs.mun.ca> NNTP-Posting-Host: sl-31.ducomm.du.edu Mime-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII X-Newsreader: WinVN 0.99.6 Xref: biosci bionet.molec-model:671 bionet.biophysics:1443 In article <1995Nov16.211834.8566@cs.mun.ca>, andrewf@cs.mun.ca> says... > > >Hello. After an absence of a few years, I am >continuing some research on the protein folding >problem. That is, I am refining a mathematical model >to predict a globular protein's tertiary structure >from its primary sequence. > >But I'm a little behind on the literature. >Can anyone point me to any recent (last 5 years) published >developments in the field? Journal review articles would >be ideal. To be more specific, I am mainly interested in >semi-empirical methods that try to exploit general >geometric characteristics of known folded proteins. >Some of these are known as distance geometry models. >I am not as interested in homologous modelling or >secondary structures. > > Thank you. >-- George Rose coauthored a paper earlier in 1995 on a computer model called LINUS which predicts 3o structure from 1o. The model exploits 3 fundamental assumptions, one of which is conserving correct sequences through 5,000 iterations. I have yet to read the paper myself, but I intend to shortly. This sounds like it applies directly to your project..good luck -- David Bovee Dept. of Chemistry University of Denver From owner-biophysics@net.bio.net Sat Nov 18 22:00:00 1995 Path: biosci!igi.tu-graz.ac.at!maass From: maass@igi.tu-graz.ac.at (Wolfgang Maass) Newsgroups: bionet.biophysics Subject: computing with noisy spiking neurons: paper in neuroprose Date: 19 Nov 1995 08:33:53 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 74 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511191635.AA28971@figids02.tu-graz.ac.at> NNTP-Posting-Host: net.bio.net The file maass.noisy-spiking.ps.Z is now available for copying from the Neuroprose repository. This is a 9-page long paper. Hardcopies are not available. FTP-host: archive.cis.ohio-state.edu FTP-filename: /pub/neuroprose/maass.noisy-spiking.ps.Z On the Computational Power of Noisy Spiking Neurons Wolfgang Maass Institute for Theoretical Computer Science Technische Universitaet Graz Klosterwiesgasse 32/2 A-8010 Graz, Austria e-mail: maass@igi.tu-graz.ac.at Abstract This article provides positive results about the computational power of neural networks that are based on a neuron model ("noisy spiking neuron") which is acceptable to most neurobiologists as being reasonably realistic for a biological neuron. In fact: this model tends to underestimate the computational capabilities of a biological neuron, since it simplifies dendritic integration. Biological neurons communicate via spike-trains, i.e. via sequences of stereotyped pulses (spikes) that encode information in their time- differences ("temporal coding"). In addition it is wellknown that biological neurons are quite "noisy". There is some "jitter" in their firing times, and neurons (as well as synapses) my fail to fire with a certain probability. It has remained unknown whether one can in principle carry out reliable computation in networks of noisy spiking neurons. This article presents rigorous constructions for simulating in real-time arbitrary given boolean circuits and finite automata on such networks. In addition we show that with the help of "shunting inhibition" such networks can simulate in real-time any McCulloch-Pitts neuron (or "threshold gate"), and therefore any multilayer perceptron (or "threshold circuit") in a reliable manner. In view of the tremendous computational power of threshold circuits (even with few layers), this construction provides a possible explanation for the fact that biological neural systems can carry out quite complex computations within 100 msec. It turns out that the assumptions that these constructions require about the shape of the EPSP's and the behaviour of the noise are surprisingly weak. This article continues the related work from NIPS '94, where we had considered computations on networks of spiking neurons without noise. The current paper will appear in Advances in Neural Information Processing Systems, vol. 8 (= Proc. of NIPS '95) . ************ How to obtain a copy ***************** Via Anonymous FTP: unix> ftp archive.cis.ohio-state.edu Name: anonymous Password: (type your email address) ftp> cd pub/neuroprose ftp> binary ftp> get maass.noisy-spiking.ps.Z ftp> quit unix> uncompress maass.noisy-spiking.ps.Z unix> lpr maass.noisy-spiking.ps (or what you normally do to print PostScript) From owner-biophysics@net.bio.net Sat Nov 18 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!Germany.EU.net!news.dfn.de!news.gwdg.de!news From: roth@sunny.mpimf-heidelberg.mpg.de (roth) Newsgroups: bionet.biophysics Subject: Re: Reference sought for ion activities Date: 19 Nov 1995 17:35:18 GMT Organization: MPI f. Med. Forschung Lines: 47 Distribution: world Message-ID: <48npsm$rl1@gwdu19.gwdg.de> References: <48ks1d$30s@lastactionhero.rs.itd.umich.edu> NNTP-Posting-Host: mac18.mpimf-heidelberg.mpg.de X-Posted-From: InterNews 1.1@mac18.mpimf-heidelberg.mpg.de X-Authenticated: roth on POP host sunny.mpimf-heidelberg.mpg.de In article <48ks1d$30s@lastactionhero.rs.itd.umich.edu> bhughes@umich.edu (Bret Hughes) writes: > You can probably find what you are looking for in: > "Electrolyte Solutions" by R.A. Robinson and R.H. Stokes, 2nd Edition, > 1959; Butterworths, London. > Unfortunately, this great resource is out of print. You may be able to > find a copy at your institution's library. > > In article , > steere@ewald.mbi.ucla.edu says... > > > >Hello, all: > > > >I am seeking a good reference which contains tables of activity > coefficients > >for salts which are common in biological systems and are measured in > >a variety of temperatures and concentrations. Where do you turn to find > >collected activity information in more detail than that provided by the > CRC > >Handbook? > > > >Thanks I've written a Mathematica package that contains tabulated values of activity coefficients from Robinson & Stokes and allows the user to fit them with a simple extended Debye-Hueckel formula. You can get this package from MathSource (ftp://mathsource.wri.com or http://www.wri.com/MathSource.html), item number 0206-198. There is also a commercial database from the National Institute of Standards and Technology, which offers more recent reference data, better fit functions (Pitzer), and is of course more complete than my little package. NIST Standard Reference Database 44: Activity and Osmotic Coefficients in Aqueous Solutions, Binary and Mixed Electrolyte systems. Available from NIST, Standard Reference Data Program, Gaithersburg, MD 20899, USA. Sincerely, Arnd Roth Abteilung Zellphysiologie Max-Planck-Institut fuer Medizinische Forschung Postfach 10 38 20, D-69028 Heidelberg, Germany http://sunny.mpimf-heidelberg.mpg.de/people/roth/ArndRoth.html From owner-biophysics@net.bio.net Sat Nov 18 22:00:00 1995 Path: biosci!neubio.sld.ar!Postmaster From: Postmaster@neubio.sld.ar (Administrador del Nodo) Newsgroups: bionet.biophysics Subject: Brain reward systems and social interactions Date: 19 Nov 1995 12:38:38 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 123 Sender: daemon@net.bio.net Distribution: world Message-ID: <815ko522@neubio.sld.ar> NNTP-Posting-Host: net.bio.net >From Postmaster Sun, 19 Nov 95 15:35:39 ARG remote from neubio >Received: by neubio.sld.ar (UUPC/PcCorreo 3.0) with UUCP; Sun, 19 Nov 95 15:35:39 ARG >Date: Sun, 19 Nov 95 15:35:39 ARG >From: Administrador del Nodo >Message-ID: <733ko327@neubio.sld.ar> >X-Mailer: UUPC/PcCorreo 3.0 >To: neuroscience@net.bio.net >Subject: Brain reward systems and social interactions > >Dear JAppel 12188, > > (upon your much cybernetic name, I hope you too bear >hominid reward systems in your central processor, so as to enjoy >the extraordinary interest of your subject question! :-) ) > **** > The sentient parenchyma is described in our old, >hylozoist local tradition as in charge of three proper functions >that act upon two general independent physical variables: > >Function One is defining stationarities upon holographic-holophonic >like interfering reverberating circuits, as Chr. Jakob show since >1906 upon a line of concepts began in 1749 but relegated in the 1890's. >Such stationarities make a structural system. Function Two is the >opening (upon long range parameters of the spacetime astronomically >traversed by the biospheric components, and by means of applying low >but coherent electroweak energies) another phase wherein non-structu- >ral phenomena, whose unstructurality prevents them to be conveyed >through structural (= informatic) channels, can be physically deter- >mined. Function Three is determining differentially these characteri- >zations on the different stationarities, turning these interactive. >The first independent physical variable is, these non-structuralities' >being experienced; the second, also taken as a non-responsive physical >variable by the natural selection forming the neurocognitive parenchyma, >is such experiencing's finding itself having its experiences in parcels; >that is, "circumstanced" into experiencing (as noema) the non-structurally >featured stationarities made by a given parcel of sentient tissue ins- >tead than, rather, another. This finding oneself circumstanced is named >in Spanish "cadacualtez". It is what makes a person (human, or of any o- >ther animal species); but an economically important historic prejudice in >our culture (the tenet that there is only science of "general things"; >particulars being unamenable to be fully known, just for such illogicity >of their cadacualteces) prevented to wonder for it except in "existen- >tial" philosophic issues; it therefore is certainly foreign to every natu- >ral-science research, at least for the present times. > >As you are probably aware, specially in the well-organized countries >which most need to rely upon the coherence of their peoples' prefigu- >rations, the neurosciences were blocked to advance research beyond Func- >tion One by a syncretic cultural myth describing non-hylozoistically such >brain tissue, that is, describing it upon a kinetized, exogenist physics. >So in the main countries the neurosciences are restricted within the >Function One, the following encaptic processes being left to theologians >or psychologists, under command of said prefigurative syncretic myth. >But this is also changing now, and so your question is very opportune. > >Now, many industries need non-Turing automata, and so our remote tradition >can opportunely offer its results (i.e., UK Patent 1,582,301). Moreover, >in the next few lusters the relaxing of integrisms (= reactions against >the modernity) with the consolidation of the planetary market, allows >to foresee two simultaneous moves: first, a recognition of cadacualtez >(German "Jemeinigkeit"; English has no term albeit "my-own-owness" has >been essayed without success; "ipseity" is not a similar, but the con- >trary concept) for cultic use, thus windowing the syncretic myth enough >for this research; and, secondly, a consecuent liberation of neuroscience >studies of the noema, as a physical resource for industrial use. > >Innovation always terrifies even academic people, but the impulse for >this process comes from economic needs and so it can foreseeably gain >a window for its growing and development. > >So you can expect having, in a few years, public neuroscience results >of researches upon the elicitation, by social interactions, of brain >reward systems activities. By the present times, in said neurosciences >so heavily limited (except in our own hylozoist traditions and perhaps >in other some individual researchers) by such exogenist physics promoted >by the syncretic myth, neither money nor stimulus was afforded to study >the differential physical productions of such sentiences. Thus, the most >you can get are some statistics of chemicals' concentrations in the >involved areas of the brain parenchyma and by no means any notice about >the physical mechanisms producing differentially such sentiences. Least, >upon re-arrangement of the system of said interactive stationarities (= >the object contents of the mental world) stirred by social interactions, >which is in what the results consist that you require. > > >By the present, not even the perceptually-stirred among those mechanisms >are studied by such neurosciences; i.e., the production of a colour in >the recall! > > >In these circumstances, the physical processes at the reward systems are >to be studied, by first time outside our remote local tradition, beyond >its general chemical elicitation at their specialized brain regions, >in their general features of basic physics (just what you cannot find now >in the present-day neurosciences abroad). > > >I hope that your interest help us to set the neurosciences of the main >countries also on a hylozoist physics that enables them to inquire and >to investigate experimentally your question, non-Turing processes in bio- >logy and in automation, and related issues; so proceeding beyond its pre- >sent confinement within the exogenist physics prefigured by the syncretic >myth. > > Cyberkisses from > Mariela > =*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*= Prof. Mariela Szirko, Centro de Investig. Neurobiologicas, Ministry of Health & Welfare, Argentine Republic; and Lab. of Electroneurobiological Res., Hospital "Dr. Jose Tiburcio Borda", Municipality of Buenos Aires, Office: Phone/Fax (54 1) 306 -7314 e-mail Standard disclaimer: Las opiniones de este mensaje son personales y no comprometen las dependencias a cargo de la firmante. Reply to THIS message, ONLY to: =*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*= From owner-biophysics@net.bio.net Sun Nov 19 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.sprintlink.net!sundog.tiac.net!daily-planet.execpc.com!usenet From: John Livingston Newsgroups: bionet.biophysics Subject: SECRET information, FREE device data, and a web page that explains more. Date: 20 Nov 1995 18:21:08 GMT Organization: S&G Enterprises Lines: 25 Message-ID: <48qguk$ind@daily-planet.execpc.com> NNTP-Posting-Host: galileo.execpc.com Mime-Version: 1.0 Content-Type: text/plain Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2 (Windows; U; 16bit) To: ALL Dear Reader, My company manufacturers versatile machinery (too big-except for the most unusual home) that can do good things for many of the people we know and the environment we share. These devices can be used to automatically separate and shrink the huge volume of bottled, barreled, canned, or otherwise containerized waste often generated while doing research. Before, all this stuff had to be handled manually...if at was handled at all. We now have a web page that tells more about what these machines might be able to do for you and yours. The page also reveals the horrible truth about Aunt Olive. It, not Aunt Olive, can be found at: http://www.execpc.com/jlivgstn/index.html Thanks for the courtesy of your time and attention. Sincerely, John Livingston S&G Enterprises N115 W19000 Edison Drive Germantown, Wisconsin 53022 (414) 251-8300 jlivgstn@execpc.com From owner-biophysics@net.bio.net Sun Nov 19 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!spool.mu.edu!daily-planet.execpc.com!usenet From: John Livingston Newsgroups: bionet.biophysics Subject: SECRET information, FREE device data, and a web page that explains more. Date: 20 Nov 1995 18:23:03 GMT Organization: S&G Enterprises Lines: 25 Message-ID: <48qh27$ind@daily-planet.execpc.com> NNTP-Posting-Host: galileo.execpc.com Mime-Version: 1.0 Content-Type: text/plain Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2 (Windows; U; 16bit) To: ALL Dear Reader, My company manufacturers versatile machinery (too big-except for the most unusual home) that can do good things for many of the people we know and the environment we share. These devices can be used to automatically separate and shrink the huge volume of bottled, barreled, canned, or otherwise containerized waste often generated while doing research. Before, all this stuff had to be handled manually...if at was handled at all. We now have a web page that tells more about what these machines might be able to do for you and yours. The page also reveals the horrible truth about Aunt Olive. It, not Aunt Olive, can be found at: http://www.execpc.com/jlivgstn/index.html Thanks for the courtesy of your time and attention. Sincerely, John Livingston S&G Enterprises N115 W19000 Edison Drive Germantown, Wisconsin 53022 (414) 251-8300 jlivgstn@execpc.com From owner-biophysics@net.bio.net Tue Nov 21 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!swrinde!elroy.jpl.nasa.gov!lll-winken.llnl.gov!enews.sgi.com!news.igc.apc.org!cdp!pledge From: The Pledge at Student Pugwash USA Newsgroups: bionet.biophysics Subject: Sign this pledge for a better world. Message-ID: Date: Tue, 21 Nov 1995 14:47:51 -0800 (PST) X-Gateway: notes@igc.apc.org Lines: 49 ---------------------------------------------------------------- DECEMBER 10, 1995 -- The Nobel Peace Prize is awarded to Professor Joseph Rotblat and the Pugwash Conferences on Science & World Affairs. TODAY -- You too can take responsibility for the world by committing to the ethical use of science and technology in society. ---------------------------------------------------------------- Imagine the impact of a million people signing this pledge IMAGINE YOU SIGNING THIS PLEDGE ---------------------------------------------------------------- "I promise to work for a better world, where science and technology are used in socially responsible ways. I will not use my education for any purpose intended to harm human beings or the environment. Throughout my career, I will consider the ethical implications of my work before I take action. While the demands placed upon me may be great, I sign this declaration because I recognize that individual responsibility is the first step on the path to peace." ---------------------------------------------------------------- To find out how you can sign the pledge or become involved in this campaign, initiated by Student Pugwash USA, visit the web site: http://www.spusa.org/pugwash/pledge.html send an e-mail message (for an automatic response) to: student-pugwash-pledge@igc.org or contact: Student Pugwash USA 815 15th Street, NW, Suite 814 Washington, DC 20005 USA 202-393-6555 pledge@spusa.org ---------------------------------------------------------------- Professor Joseph Rotblat: "We young and old must nurture a vision of a better world in the next century, a world without war, a society based on care and equity, a community that will protect the environment. And we should make it our task to turn this vision into reality." ---------------------------------------------------------------- (Help gather a million signatures on this pledge! Please forward this message to all applicable locations, and place a link to the Web page on your homepage.) From owner-biophysics@net.bio.net Tue Nov 21 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: baowowmeow@aol.com (BaoWowMeow) Newsgroups: bionet.biophysics Subject: "F-light A Quantum Fiction Novel", physicists query Date: 21 Nov 1995 18:26:58 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 11 Sender: root@newsbf02.news.aol.com Message-ID: <48tn82$hk1@newsbf02.news.aol.com> Reply-To: baowowmeow@aol.com (BaoWowMeow) NNTP-Posting-Host: newsbf02.mail.aol.com Does anyone here have "Flight A Quantum Fiction novel" by Vanna Bonta? It's fiction but I would be interested in a physicist's opinions. A stretched mind will never regain its shape, and I'm wondering if it's just good fiction or if there is any fact to it as pertains to the "observer affecting the experiment" (i.e. molecules and thus coincidence of events and reality). Bao There's a Home Page on the book, chapters, pictures, etc. There's also a chat room. Anyone on AOL, it might be interesting to meet int here and exchange some theories. I'm very curious to hear from scientists. The site address is: http://home.aol.com.airaflight/ From owner-biophysics@net.bio.net Tue Nov 21 22:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!sdd.hp.com!swrinde!newsfeed.internetmci.com!in1.uu.net!csn!nntp-xfer-2.csn.net!yuma!purdue!mozo.cc.purdue.edu!aim.et.iupui.edu!NewsWatcher!user From: lipids@nowhere.com (Stillwell's Lab) Newsgroups: bionet.biophysics Subject: Pyrene Fluorescence Date: Tue, 21 Nov 1995 16:52:40 -0600 Organization: IUPUI Biology Lines: 18 Message-ID: NNTP-Posting-Host: 134.68.79.62 Greetings to all, Our research is concerning the Pyrene Fluorescence. We are using KG1A, a leukemic cell line, to detect differences in membrane fluidity. Our major problem is that although we can record the monomer fluorescence, we cannot detect the epimer fluorescence that pyrene is supposed to exhibit. Our methods are based on the paper published by Treen et al., 'Effect of Docosahexaenoic Acid on Membrane Fluidity and the Function in INtact Cultured Y-79 Retinoblastima Cells', Arch Biochem Biophys, vol. 294, No.2, May 1, pp. 564-570. Before measuring fluorescence, we also degassed our cells in pyrene solution for 15 minutes and we have used higher concentrations of pyrene. Any suggestions would be welcome. Thank you for your time, Alfred C. Dumaual acdumaua@indyvax.iupui.edu Rob Pyatt repyatt@indyvax.iupui.edu From owner-biophysics@net.bio.net Tue Nov 21 22:00:00 1995 Path: biosci!daresbury!not-for-mail From: bmgazit@weizmann.weizmann.ac.il (Ehud Gazit) Newsgroups: bionet.biophysics Subject: Coordinates of membrane models Date: 22 Nov 1995 15:26:07 -0000 Lines: 21 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <48vfef$jfc@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk Hello Netters, I am looking for coordinates of phospholipid bilayer membranes simulations. I have a special interest in models of acidic membranes (e.g. PS, PS/PC, PG/PC etc.) without cholesterol. Can someone help me? Thank you. Ehud. ************************************************* * Ehud Gazit * * Department of Membrane Research & Biophysics * * Weizmann Institute of Science * * Rehovot 76100, Israel * * Tel:+972-8-342712, Fax:+972-8-344112 * * e-mail:bmgazit@weizmann.weizmann.ac.il * * bmgazit@membran1.weizmann.ac.il * ************************************************* From owner-biophysics@net.bio.net Tue Nov 21 22:00:00 1995 Path: biosci!daresbury!yama.mcc.ac.uk!zippy.dct.ac.uk!str-ccsun!strath-cs!ftel.co.uk!bham!bhamcs!news.ox.ac.uk!news Newsgroups: bionet.molec-model,bionet.biophysics Subject: Re: literature on protein folding models? Message-ID: <30B324FE.41C6@bioch.ox.ac.uk> From: Geoff Barton Date: Wed, 22 Nov 1995 13:26:22 +0000 References: <1995Nov16.211834.8566@cs.mun.ca> Organization: Laboratory of Molecular Biophysics NNTP-Posting-Host: weevil.biop.ox.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b2 (X11; I; IRIX 5.3 IP22) Lines: 22 Xref: biosci bionet.molec-model:675 bionet.biophysics:1452 Andrew Foster wrote: > > But I'm a little behind on the literature. > Can anyone point me to any recent (last 5 years) published > developments in the f