From owner-biophysics@net.bio.net Mon Jan 01 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!newsfeed.internetmci.com!chi-news.cic.net!newsjunkie.ans.net!inet.d48.lilly.com!sherwood.d46.lilly.com!gfn Newsgroups: bionet.biophysics Subject: Industrial Job Opening Message-ID: <1996Jan2.121424.7279@inet.d48.lilly.com> From: Greg Needham Date: 2 Jan 96 12:14:23 EST Distribution: world Organization: Eli Lilly and Co. Nntp-Posting-Host: sherwood.d46.lilly.com X-UserAgent: Version 1.1.3 X-XXMessage-ID: X-XXDate: Tue, 2 Jan 96 12:15:27 GMT Lines: 17 Associate Formulations Chemist. An associate formulations chemist is needed for the development and characterization of formulations for new animal health products. This scientist will provide laboratory support for the identification of new dosage forms or delivery systems for synthetic organic compounds and fermentation products. Preference will be given to qualified candidates having a BS or MS degree and relevant experience in physical chemistry, chemical engineering, pharmaceutics, pharmaceutical sciences, or a related field. Interested individuals should please respond with a resume to: Mr. Doug Whiteman Lilly Research Laboratories 2001 West Main Street Greenfield, IN 46140 Eli Lilly and Company is an equal opportunity employer. From owner-biophysics@net.bio.net Mon Jan 01 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!chi-news.cic.net!news.luc.edu!orion.it.luc.edu!scukier From: scukier@orion.it.luc.edu (Sam Cukierman) Newsgroups: bionet.biophysics Subject: Ankyrin+spectrin Date: 2 Jan 1996 18:36:01 GMT Organization: Loyola University Chicago Lines: 5 Message-ID: <4cbtuh$gih@artemis.it.luc.edu> NNTP-Posting-Host: 147.126.1.9 NNTP-Posting-User: scukier X-Newsreader: TIN [version 1.2 PL2] Could you tell me if the cytoskeleton proteins (brain or red blood cells) ankyrin and spectrin are commercially available? What company sells them? Many thanks. Sam Cukierman, M.D., Ph.D. Physiology/LUMC From owner-biophysics@net.bio.net Tue Jan 02 22:00:00 1996 Path: biosci!TELEPAC.PT!pftavares From: pftavares@TELEPAC.PT (Paulo Freitas Tavares) Newsgroups: bionet.biophysics Subject: Help in Scientific Graphics ! Date: 3 Jan 1996 07:36:45 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601031612.QAA19018@mail.telepac.pt> NNTP-Posting-Host: net.bio.net I have the necessity of creating graphics with only the Y axis with the aspect caricaturated below. It isn't the case, but is a good example, the distribution of the heights of a group of people. The Y axis indicates the centimetres (or feet, or inches) the density of the points (the width of the band) the number of people with that height. Most probably due to my ignorance I can't achieve this with such elaborate programs as EXCEL 5 or SPSS for Windows. Is there any way of doing this with these programs? Are there any good Shareware programs capable of such a simple task? Where could I get them? * ** **** **** ******* ********** ************* *********** ******** ***** *** ** I will sincerely appreciate any comments, suggestions or any kind of help. Paulo Freitas Tavares, MD pftavares@telepac.pt From owner-biophysics@net.bio.net Tue Jan 02 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!newsfeed.internetmci.com!in2.uu.net!munnari.OZ.AU!news.mel.connect.com.au!yarrina.connect.com.au!news.mel.aone.net.au!inferno.mpx.com.au!jolt!andykirk From: andykirk@jolt.mpx.com.au (Andrew Kirkpatrick) Newsgroups: bionet.biophysics Subject: Cell Membrane Semiconductivity Date: 3 Jan 1996 11:43:01 GMT Organization: Microplex Pty Ltd Lines: 14 Message-ID: <4cdq45$vg5@inferno.mpx.com.au> NNTP-Posting-Host: jolt.mpx.com.au X-Newsreader: TIN [version 1.2 PL2] I remember reading, though I can't remember where, that the cell membrane, essentially a phospholipid bilayer, is semi-conductive. Is this true, and if so, what conditions are associated with a cell membrane a) conducting, b) insulating. In other words, what factors influence the conductivity of cell membranes? I guess if the membrane were somehow made impermeable ions couldn't carry charge accross it, but what about conductivity along the surface? Any idea/fact/oids would be greatly appreciated. From owner-biophysics@net.bio.net Tue Jan 02 22:00:00 1996 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!in2.uu.net!news.emi.com!pauling.wadsworth.org!tivol From: tivol@news.wadsworth.org (William Tivol) Newsgroups: bionet.biophysics Subject: Re: Cell Membrane Semiconductivity Date: 3 Jan 1996 19:11:04 GMT Organization: Wadsworth Center, NY Health Dept. Lines: 13 Message-ID: <4cekc8$r7e@pauling.wadsworth.org> References: <4cdq45$vg5@inferno.mpx.com.au> <4cejun$qn6@pauling.wadsworth.org> NNTP-Posting-Host: alcor.wadsworth.org X-Newsreader: TIN [version 1.2 PL2] William Tivol (tivol@news.wadsworth.org) wrote: : A good book on this : subject is Channels, Carriers, and Pumps--An Introduction to Membrane : Transport by Wilfred D. Stern, Oops! Wilfred D. Stein. : published by Academic Press, ISBN 0-12- : 665045-4. I reviewed the book, but have no financial interest in it or in : Academic Press. : Yours, : Bill Tivol From owner-biophysics@net.bio.net Tue Jan 02 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!news.emi.com!pauling.wadsworth.org!tivol From: tivol@news.wadsworth.org (William Tivol) Newsgroups: bionet.biophysics Subject: Re: Cell Membrane Semiconductivity Date: 3 Jan 1996 19:03:51 GMT Organization: Wadsworth Center, NY Health Dept. Lines: 25 Message-ID: <4cejun$qn6@pauling.wadsworth.org> References: <4cdq45$vg5@inferno.mpx.com.au> NNTP-Posting-Host: alcor.wadsworth.org X-Newsreader: TIN [version 1.2 PL2] Andrew Kirkpatrick (andykirk@jolt.mpx.com.au) wrote: : I remember reading, though I can't remember where, that the cell : membrane, essentially a phospholipid bilayer, is semi-conductive. Actually, semi-permeable. : Is this true, and if so, what conditions are associated with a : cell membrane a) conducting, b) insulating. : In other words, what factors influence the conductivity of cell : membranes? Most biological membranes have their conductances controlled by specific molecules (usually proteins or peptides) which transport particular ions. These fall into two broad classifications--carriers and channels. The channels can be active or passive, and can either transport net charge or exchange ions of equal charge--giving a net result of no charge change. These transport molecules can be regulated by many factors, such as pH, transmembrane voltage, allosteric effectors, etc. A good book on this subject is Channels, Carriers, and Pumps--An Introduction to Membrane Transport by Wilfred D. Stern, published by Academic Press, ISBN 0-12- 665045-4. I reviewed the book, but have no financial interest in it or in Academic Press. Yours, Bill Tivol From owner-biophysics@net.bio.net Wed Jan 03 22:00:00 1996 Path: biosci!notes.compuserve.com!John_Durkin.CEPHALON From: John_Durkin.CEPHALON@notes.compuserve.com Newsgroups: bionet.biophysics Subject: CMCs and co-solvents Date: 4 Jan 1996 09:52:05 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <960104174746_702420.204300_BHD71-40@CompuServe.COM> NNTP-Posting-Host: net.bio.net Detergent-solubilized membrane proteins are notoriously labile, but can sometimes be stabilized by addition of co-solvents such as glycerol. It is often recommended that chromatography of such proteins be performed above the critical micelle concentration (CMC) of the detergent. My question is, how does the co-solvent affect the CMC? I expect that, in 20% glycerol (for example), the CMC for a given detergent will be lower than in water, because the water activity is lower. But how much? Has anyone determined CMCs in such complicated systems? Any information along these lines will be appreciated. John T. Durkin / Cephalon Inc. / 145 Brandywine Parkway / West Chester, PA 19380-4245 (610)344-0200 X160 work / (610)344-0065 fax EMB: jdurkin.cephalon@notes.ccmail.compuserve.com From owner-biophysics@net.bio.net Wed Jan 03 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!swrinde!cssun.mathcs.emory.edu!news.cc.emory.edu!news.cc.emory.edu!not-for-mail From: dhughes@larry.cc.emory.edu (David Clarence Hughes) Newsgroups: bionet.biophysics Subject: chameleons Date: 3 Jan 1996 19:20:24 -0500 Organization: Emory University Lines: 5 Message-ID: <4cf6g8$q37@larry.cc.emory.edu> NNTP-Posting-Host: larry.cc.emory.edu X-Newsreader: TIN [version 1.2 PL2] a basic question: what makes a chameleon change color? thank you, david hughes From owner-biophysics@net.bio.net Thu Jan 04 22:00:00 1996 Path: biosci!agate!dog.ee.lbl.gov!ihnp4.ucsd.edu!swrinde!newsfeed.internetmci.com!newsxfer2.itd.umich.edu!newsxfer.itd.umich.edu!news.itd.umich.edu!usenet From: Rick Neubig Newsgroups: bionet.biophysics Subject: Re: Help in Scientific Graphics ! Date: Wed, 03 Jan 1996 13:14:20 -0500 Organization: University of Michigan Lines: 56 Message-ID: <30EAC77C.5D01@umich.edu> References: <199601031612.QAA19018@mail.telepac.pt> NNTP-Posting-Host: warbler.med.umich.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b4 (Win95; I) To: Paulo Freitas Tavares CC: hmotulsky@graphpad.com We use GraphPad Prism for this. They call it a "column scatter" graph. It is a Windows program. It's not shareware but you can get a demo version from their Web site at: http://www.graphpad.com Rick Paulo Freitas Tavares wrote: - - I have the necessity of creating graphics with only the Y axis with - the aspect caricaturated below. - It isn't the case, but is a good example, the distribution of the - heights of a group of people. The Y axis indicates the centimetres (or feet, - or inches) the density of the points (the width of the band) the number of - people with that height. - Most probably due to my ignorance I can't achieve this with such - elaborate programs as EXCEL 5 or SPSS for Windows. - Is there any way of doing this with these programs? Are there any - good Shareware programs capable of such a simple task? Where could I get them? - - * - ** - **** - **** - ******* - ********** - ************* - *********** - ******** - ***** - *** - ** - - I will sincerely appreciate any comments, suggestions or any kind of - help. - - Paulo Freitas Tavares, MD - pftavares@telepac.pt -- _________________________________________________________ Rick Neubig RNeubig@umich.edu University of Michigan Phone (313) 763-3650 http://www.umich.edu/~rneubig FAX (313) 763-4450 From owner-biophysics@net.bio.net Thu Jan 04 22:00:00 1996 Path: biosci!ipc.pku.edu.cn!ldw From: ldw@ipc.pku.edu.cn (ldw) Newsgroups: bionet.biophysics Subject: I want to experimental data of folding intermediates Date: 5 Jan 1996 03:40:48 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Netters, At presents I predict some folding nuleated site by searching the PDB. Now I need some experimental data to test them. All kinds of experimental data which have the detail information about where is the folding nuleaated site or folding initiation site are welcome. (the information like the paper on Nature Vol.269 Page 192-197, which states that N and C terminal helix fold ealier. This kind of information is most favorate). I will summarize what is intereting I receive to this list. Thanks in advance, Dawei ----------< *** Dawei LIN *** >---------------- Ph.D student of Molecular Design Lab Institute of Physical chemistry Peking University Beijing 100871 P.R.China Phone: 86-10-2751490 Fax: 86-10-2751725 Email: ldw@pschnetware.pku.edu.cn ldw@ipc.pku.edu.cn URL: http://www.ipc.pku.edu.cn/ldw/ldw.htm ------------------------------------------------ From owner-biophysics@net.bio.net Thu Jan 04 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!usenet.eel.ufl.edu!news.ultranet.com!bigboote.WPI.EDU!news3.near.net!news.ner.bbnplanet.net!das-news2.harvard.edu!oitnews.harvard.edu!cmcl2!newsserv.cs.sunysb.edu!news.cc.sunysb.edu!delbruck!culli From: culli@delbruck (David Cullinan) Newsgroups: bionet.biophysics Subject: Acetylcholine structure? Date: 4 Jan 1996 19:43:17 GMT Organization: State University of New York at Stony Brook Lines: 14 Message-ID: <4chakl$of@abel.cc.sunysb.edu> NNTP-Posting-Host: delbruck.pharm.sunysb.edu X-Newsreader: TIN [version 1.2 PL2] Greetings! Does anyone know where I could obtain pdb file atomic coordinates for acetylcholine, carbamylcholine, bethanechol, nicotine, curare, and/or atrophine? I'm primarily interested in their structural orientation when binding to the acetylcholine receptor. I know I can find 2-dimensional drawings of these in any decent reference book, but I'd like to get pdb coordinates that I could input to a program like Insight or MIDAS. Many thanks. ______________________________________________________________________ David Cullinan (Graduate student) Dept of Biophysics email: culli@delbruck.pharm.sunysb.edu SUNY Stony Brook http://www.pharm.sunysb.edu/~culli/home.html From owner-biophysics@net.bio.net Thu Jan 04 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!usenet From: Daniel Nolan Newsgroups: bionet.biophysics Subject: help with grants Date: Fri, 05 Jan 1996 17:31:54 -0500 Organization: Cyber Eyes Lines: 5 Message-ID: <30EDA6DA.5D24@cyberiz.com> NNTP-Posting-Host: dialin4.parrett.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b4 (Win95; I) I have a friend who is looking for grant/scholarship money for graduate study in Biophysics. Any information would be very useful Thanks Dan Nolan From owner-biophysics@net.bio.net Thu Jan 04 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!newsxfer2.itd.umich.edu!news.itd.umich.edu!usenet From: Rick Neubig Newsgroups: bionet.biophysics Subject: Re: Acetylcholine structure? Date: Fri, 05 Jan 1996 16:36:00 -0500 Organization: University of Michigan Lines: 35 Message-ID: <30ED99C0.49B1@umich.edu> References: <4chakl$of@abel.cc.sunysb.edu> NNTP-Posting-Host: warbler.med.umich.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b4 (Win95; I) David Cullinan wrote: > > Greetings! > > Does anyone know where I could obtain pdb file atomic coordinates for > acetylcholine, carbamylcholine, bethanechol, nicotine, curare, and/or > atrophine? I'm primarily interested in their structural orientation > when binding to the acetylcholine receptor. There is at least one published report on the structure of Ach when bound to its receptor. This was determined by transfer NOE. I haven't followed this field enough to know the current status of the work but you can get the initial references from the textbook page 22 Principles of Drug Action 3rd edition Pratt WB and Taylor P Churchill Livingstone 1990 > I know I can find 2-dimensional drawings of these in any decent reference > book, but I'd like to get pdb coordinates that I could input to a > program like Insight or MIDAS. > Many thanks. > ______________________________________________________________________ > David Cullinan (Graduate student) > Dept of Biophysics email: culli@delbruck.pharm.sunysb.edu > SUNY Stony Brook http://www.pharm.sunysb.edu/~culli/home.html -- _________________________________________________________ Rick Neubig RNeubig@umich.edu University of Michigan Phone (313) 763-3650 http://www.umich.edu/~rneubig FAX (313) 763-4450 From owner-biophysics@net.bio.net Thu Jan 04 22:00:00 1996 Path: biosci!UMDNJ.EDU!parness From: parness@UMDNJ.EDU (Jerome Parness) Newsgroups: bionet.biophysics Subject: Postdoctoral position - Intracellular Calcium Date: 5 Jan 1996 10:25:16 -0800 Organization: UMDNJ Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: <30ED977F.3848@umdnj.edu> NNTP-Posting-Host: net.bio.net Postdoctoral position immediately available for experienced individual interested in the regulation of discreet pools of intracellular calcium in skeletal muscle, cardiac muscle and brain. Join young lab for exciting new directions. Techniques of membrane biochemistry, radioactive ligand binding assays, cell culture, calcium flux assays, confocal microscopy and calcium indicator imaging, and single cell electrophysiology highly desirable. This position is partly grant funded and partly departmentally funded. Pay scale is NIH. For further information conatact Jerome Parness MD PHD and send CV to parness@umdnj.edu or, via snail mail: Jerome Parness MD PhD Departments of Anesthesia, Pharmacology and Pediatrics UMDNJ-Robert Wood Johnson Medical School Clinical Academic Building/Suite 3100 125 Paterson Street New Brunswick, NJ 08903-0019 voice (908)235-4824 or (908)937-8841 FAX (908)235-4073 or (908)418-8492 From owner-biophysics@net.bio.net Thu Jan 04 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!news00.sunet.se!sunic!news99.sunet.se!nntp-trd.UNINETT.no!daresbury!not-for-mail From: psb@mole.bio.cam.ac.uk (Paul. S. Brookes.) Newsgroups: bionet.biophysics Subject: Re: Cell Membrane Semiconductivity Date: 5 Jan 1996 15:34:12 -0000 Lines: 56 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4cjgdk$da3@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk William Tivol (tivol@news.wadsworth.org) wrote: >Andrew Kirkpatrick (andykirk@jolt.mpx.com.au) wrote: >>I remember reading, though I can't remember where, that the cell >>membrane, essentially a phospholipid bilayer, is semi-conductive. > > Actually, semi-permeable. > >>Is this true, and if so, what conditions are associated with a >>cell membrane a) conducting, b) insulating. In other words, what >>factors influence the conductivity of cell membranes? > >Most biological membranes have their conductances controlled by >specific molecules (usually proteins or peptides) Hmmmmm.... I think the question is more about the properties of the PL bilayer itself rather than its associated proteins or carriers. Certainly, closed PL membranes (such a Egg PC liposomes) can hold onto ions like sodium or potassium for a long time (they diffuse out over a time-course of days). Mainly this is due to such ions having a large hydration shell that cannot be satisfied by the hydrophobic interior of the membrane, so they're unlikely to enter the membrane in the first place. The exception to the rule is protons. Protons (or H+/OH- pairs) can cross membranes at rates 7-10 orders of magnitude greater than Na+ or K+. There's loads of literature out there on the mechanism of proton conductance by membranes, with the majority of opinion now leaning towards a "water wire" model, in which transiently hydrogen bonded chains of water molecules can span the membrane, taking on a proton at one end, and releasing one at the other end. As to what mediates this proton conductance, some membrane compositional diferences (fatty acid side chain unsaturation, PL head groups) might favour water wires more than others, or proteins may be involved. In the case of the mitochondrial proton leak (uncoupling of ox-phos), the Ad-Nuc Translocase may be involved, or free-fatty acids may play a role, though whether protons leak at the protein:lipid interface, or just through lipid or protein alone remains to be seen. Authors to look out for in the literature are Deamer, D. W., Hinkle, P.C., Krishnamoorthy, G., Brand, M. D., Garlid, K. Regards, PSB [][][][][][][][][][][][][][][][][][][][][][][][][][][][] Paul. S. Brookes E-mail:psb@mole.bio.cam ac.uk Dep't of Biochemistry, Tel: 01223 333649 Univrsity of Cambridge, Fax: 01223 333345 Tennis Court Rd, Cambridge, CB2 1QW, UK http://www.bio.cam.ac.uk/lab/brand/people/paul/ [][][][][][][][][][][][][][][][][][][][][][][][][][][][] From owner-biophysics@net.bio.net Thu Jan 04 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news00.sunet.se!sunic!news99.sunet.se!nntp-trd.UNINETT.no!daresbury!not-for-mail From: psb@mole.bio.cam.ac.uk (Paul. S. Brookes.) Newsgroups: bionet.biophysics Subject: Area of a single phospholipid Date: 5 Jan 1996 15:04:29 -0000 Lines: 59 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4cjelt$b4g@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk I'm doing calculations that require knowledge of the area occupied by a phospholipid in a bilayer. This concept can be interpreted in 2 ways:- <> It's the surface area occupied by a single PL head group when viewed looking down onto the plane of a lipid bilayer, in which case to get the area of bilayer from a known number of lipid molecules, one has to multiply single PL area by PL number, then halve the answer (equivalent to folding the monolayer in two). or <> It is the amount of bilayer area made by a single PL (a silly concept if ever there was one), obviously having been calculated from an area of bilayer divided by the number of PL molecules present, without correcting for lamellarity. The literature gives a range of values:- New, R.R.C. In "Liposomes, a practical approach" (IRL Press, UK, 1990) pp1-31 "the headgroup of PC occupies an area of the membrane (42 A^2)..." (sq angstroms) Cullis, P.L. et al. In "Biochemistry of Lipids, Lipoproteins & Membranes" (Elsevier, Holland, 1991) pp1-40 "... assuming a surface area per PL molecule of 0.6nm^2" (this is equivalent to 30A^2) Johnson, S.M. Biochim. Biophys. Acta. Vol 307, pp27-41 (1973) "...surface area of the bilayer per PL molecule of 89 A^2" Hope, M. J. et al. Biochim. Biophys. Acta. Vol 812, pp55-65 (1985) "area per PL molecule of 0.6nm^2" (= 30A^2) Schieren, H. et al. Biochim. Biophys. Acta. Vol 542, pp137-153 (1978) "area per molecule of 87 A^2" Please, if you know which figure should be used, whether they refer to monolayer/bilayer areas, or have any figures of your own, I'd be most grateful for further information, as having calculations out by a factor of 2 is just a little bit outside my error limits! Many thanks in advance, PSB [][][][][][][][][][][][][][][][][][][][][][][][][][][][] Paul. S. Brookes E-mail:psb@mole.bio.cam ac.uk Dep't of Biochemistry, Tel: 01223 333649 Univrsity of Cambridge, Fax: 01223 333345 Tennis Court Rd, Cambridge, CB2 1QW, UK http://www.bio.cam.ac.uk/lab/brand/people/paul/ [][][][][][][][][][][][][][][][][][][][][][][][][][][][] From owner-biophysics@net.bio.net Fri Jan 05 22:00:00 1996 Path: biosci!rpslmc.edu!wniles From: wniles@rpslmc.edu ("Walter D. NIles") Newsgroups: bionet.biophysics Subject: Re: Cell Membranes as Semiconductors Date: 6 Jan 1996 14:11:20 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net One useful guide for comparing cell membranes and semiconductors is the article by Alan Finkelstein and Alexander Mauro in the Handbook of Physiology, vol. 1, published in 1981 by the American Physiological Society. They compare the "negative conductance" and other excitability phenomena of the Hodkin-Huxley-Goldman equivalent circuit model for a nerve membrane with a p-n junction. The essential feature is the rectification of current due to either the voltage-dependence of channel opening and closing or the permeation physics of the individual channels. It is worthwhile to note that this is only a comparison. They point out that a Boltzmann distribution of a channel population between open and closed states is identical mathematically to the simplest ideal distribution of charge carriers across the p-n junction, but that is the extent of the similarity, as the mechanistic basis for each is very different. It may also be worthwhile to note that nonlinear i-V behavior is a characteristic of lipid bilayers modified with some sort of mobile charge carrier such as an ion channel or a hydrophobic ion. Lipid bilayers themselves are exhibit remarkably linear i-V characteristics and the parallel RC equivalent circuit is a good model. Nonlinearities, equivalent to the saturation of diode current (or transconductance) with voltage , are absent from pure bilayers. Walter D. Niles From owner-biophysics@net.bio.net Fri Jan 05 22:00:00 1996 Path: biosci!agate!overload.lbl.gov!lll-winken.llnl.gov!uwm.edu!cs.utexas.edu!news.sprintlink.net!ralph.vnet.net!icagen From: castle@icagen.com (Neil A. Castle) Newsgroups: bionet.jobs,bionet.biophysics,sci.research.careers,misc.jobs.offered,triangle.jobs,biz.jobs.offered Subject: Jobs: Chemistry, Electrophysiology, Molecular and Cell Biology, Pharmacology Date: Sat, 06 Jan 96 20:58:15 GMT Organization: ICAgen Lines: 53 Message-ID: <4cmnek$f36@ralph.vnet.net> NNTP-Posting-Host: icagen.com X-Newsreader: News Xpress Version 1.0 Beta #4 Xref: biosci bionet.biophysics:1558 sci.research.careers:8203 misc.jobs.offered:506343 biz.jobs.offered:105347 ICAgen, Inc., a RTP-based biopharmaceutical company focused on ion channel drug discovery is expanding its biology team and initiating its chemistry group. · Scientists with the following qualifications are being immediately hired. BIOLOGY Electrophysiology Ph.D. Electrophysiologists at ICAgen lead our biology team using biophysical, molecular and computational approaches in the study of ion channel modulation. Successful candidates must be experienced in a wide range of electrophysiological methods. Molecular Biology Ph.D. As a scientist in this group, you will take a leading role in exploring ion channel subtypes involved in disease. You must have experience in cloning, expression, mutagenesis and tissue distribution studies. Cell Biology/Pharmacology 2 positions - Ph.D., MS/BS You will be involved in all phases of ion channel drug discovery, including the development and completion of high-throughput screening assays. Experience in assay development is required. CHEMISTRY 3 positions - Ph.D. ICAgen is recruiting a core team of chemists to initiate its organic synthesis group. Successful candidates with be organic chemists with either - 3-5 years of medicinal chemistry experience, plus knowledge of molecular simulation methods and combinatorial chemistry, or - postdoctoral experience encompassing either molecular simulation methods or combinatorial chemistry techniques, or - direct experience in molecular diversity/combinatorial chemistry (both solution or solid phase synthesis). ICAgen provides competitive salaries, a full benefits package and equity in this privately held Company. ICAgen is an equal opportunity employer. Please send resumes, clearly stating specific position for which you are applying to ICAgen, Inc., Human Resources P.O. Box 14487, RTP, N.C. 27709 Alternatively, E-mail resume (in ascii format)to; castle@icagen.com From owner-biophysics@net.bio.net Fri Jan 05 22:00:00 1996 Path: biosci!agate!dog.ee.lbl.gov!news.cs.utah.edu!cc.usu.edu!davef From: davef@cc.usu.edu Newsgroups: bionet.biophysics Subject: ion channel modelling software? Message-ID: <1996Jan5.174802.70614@cc.usu.edu> Date: 5 Jan 96 17:48:02 MDT Organization: Utah State University Lines: 14 Can anyone recommend a some good commercial software for making stochastic models, in particular, kinetic models of voltage-gated ion channels? A year or so ago there was a thing called 'Axon Engineer', and I have heard of something called 'SCOP' or something like that, but I have no further information. I need something in which one can define the number of states, and then state transitions and voltage dependencies, at least. Please post or (preferably) email suggestions. davef@cc.usu.edu From owner-biophysics@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!bloom-beacon.mit.edu!gatech!news.mathworks.com!tank.news.pipex.net!pipex!news.uoregon.edu!news.u.washington.edu!lamontg From: lamontg@u.washington.edu (Lamont Granquist) Newsgroups: bionet.biophysics,fj.sci.physics,sci.physics,sci.physics.electromag,sci.physics.particle Subject: Re: Information on glueballs, quarks, and anti-quarks Date: 8 Jan 1996 22:33:41 GMT Organization: University of Washington Lines: 9 Message-ID: <4cs645$p36@nntp3.u.washington.edu> References: <4c7cnp$gnm@nn.fast.net> <4crp4u$8p1@sdcc12.ucsd.edu> <4cs34s$6la@guitar.ucr.edu> NNTP-Posting-Host: saul8.u.washington.edu NNTP-Posting-User: lamontg Xref: biosci bionet.biophysics:1567 sci.physics:116930 sci.physics.electromag:7575 sci.physics.particle:5631 the latest issue of _science_ has a couple pages on glueballs (actually i think it might have been the last december issue and not the current one, but pretty darn recent...) -- Lamont Granquist E-mail: lamontg@u.washington.edu ICBM: 47 39'23"N 122 18'19"W There comes a point, I'm afraid, where you begin to suspect that if there's any _real_ truth, it's that the entire multidimentional infinity of the Universe is almost certainly being run by a bunch of maniacs -- Douglas Adams From owner-biophysics@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!galaxy.ucr.edu!not-for-mail From: baez@guitar.ucr.edu (john baez) Newsgroups: bionet.biophysics,fj.sci.physics,sci.physics,sci.physics.electromag,sci.physics.particle Subject: Re: Information on glueballs, quarks, and anti-quarks Date: 8 Jan 1996 13:42:52 -0800 Organization: University of California, Riverside Lines: 59 Message-ID: <4cs34s$6la@guitar.ucr.edu> References: <4c7cnp$gnm@nn.fast.net> <4crp4u$8p1@sdcc12.ucsd.edu> NNTP-Posting-Host: guitar.ucr.edu Xref: biosci bionet.biophysics:1565 sci.physics:116923 sci.physics.electromag:7574 sci.physics.particle:5628 >In article <4c7cnp$gnm@nn.fast.net> mpb@fast.net writes: >>The glueball is what I am writing my research project on. Why in the world would you ask a question about glueballs on a biophysics newsgroup? Oh well... Here's some stuff from week 68 of my series, This Week's Finds in Mathematical Physics: 4) Frank Close, Are glueballs and hybrids found?, preprint available as hep-ph/9509245 style file sprocl.sty required (available from hep-ph). To appear in Proceedings of Hadron95. J. Sexton, A. Vaccarino, D. Weingarten, Numerical evidence for the observation of a scalar glueball, preprint available as hep-lat/9510022. Thanks go to Greg Kilcup for bringing these to my attention. Have they found a glueball??? That would be really exciting. What's a glueball, you ask? Well, quantum chromodynamics, our best theory of the strong force, says that that the strong force is carried by particles called "gluons". Like electromagnetism, the strong force is a gauge field, but it's a nonabelian gauge field, so the gluons themselves have charge, or "color". Thus they interact in a nonlinear way. This is what lets them bind together quarks in such a tight way. But perhaps, in addition to pairs of quarks and antiquarks held together by gluons --- i.e., mesons --- and triples of quarks held together by gluons --- i.e., baryons --- there could be short-lived assemblages consisting entirely of gluons, held together by their self-interactions. These are called glueballs, but we don't know if these exist. However, to my surprise, it turns out that there are now some candidates out there! The first paper suggests that the f_0(1500), a neutral spin-0 particle with mass around 1500 MeV, is a glueball. The second paper argues instead that this is basically a quark-antiquark pair (made of a strange quark and a strange antiquark... where "strange" is the technical name for one of the 6 quarks!). It presents evidence from a numerical simulation and argues that the "theta" or f_J(1710), a spin-zero particle with mass 1710 MeV, is a glueball. It's tough to do nonperturbative computations in nonlinear gauge field theories --- basically one needs to approximately compute a path integral, using Monte Carlo technique, approximating spacetime by a lattice (in this case, a 16 x 16 x 16 x 24 lattice). Computing the properties of a glueball and matching it with an experimentally observed particle would be a marvelous confirmation of quantum chromodynamics. In addition, I find there to be something charming about the idea that in a nonabelian gauge theory we could have a particle made simply of the gauge field itself. ------ If you don't know how to get papers from hep-ph or hep-lat, send email with subject header help to hep-ph@xxx.lanl.gov and hep-lat@xxx.lanl.gov. From owner-biophysics@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news.ultranet.com!bigboote.WPI.EDU!bigwpi.WPI.EDU!defronzo From: defronzo@wpi.edu (CDP Online) Newsgroups: bionet.biophysics Subject: MEETING: Cancer Biotech Oct. 26-28 '96 Nice, France Date: 8 Jan 1996 20:07:30 GMT Organization: Worcester Polytechnic Institute Lines: 58 Message-ID: <4crti2$snm@bigboote.WPI.EDU> NNTP-Posting-Host: bigwpi.wpi.edu X-Newsreader: TIN [version 1.2 PL2] CALL FOR ABSTRACTS 3rd International Symposium on CANCER BIOTECHNOLOGY IN PREDICTIVE ONCOLOGY AND THERAPY DIAGNOSTIC & PROGNOSTIC INDICATORS October 26-28, 1996 Nice, France Sponsors : International Society for Preventive Oncology - in cooperation with The World Health Organization University of Massachusetts Medical Center University of Illinois College of Medicine National Cancer Institute Advanced Biotechnology Center International Agency for Research on Cancer Massachusetts Biotechnology Council Clinicians, scientists and technicians of medical institutions from around the world will be meeting to appraise and update knowledge of and experience with new approaches to identification of carcinogenic exposure, risk assessment, genetic and metabolic influences, prognosis, early diagnosis and therapy of hematologic neoplasms and solid tumors. The symposium has been planned with the goal of fostering cooperation between universities, institutes and enterprises engaged in the research, development and implementation of advanced biotechnologies in laboratory and clinical practice. New products, developments and applications will be presented within a framework of podium sessions, posters and technical displays. The abstracts and papers of experimental and clinical reports will be published in the peer-review journal, Cancer Detection and Prevention. DEADLINE FOR ABSTRACTS = June 28, 1996. For more information about the program: CDP Online: http://www.ummed.edu:8000/pub/e/emerithe/ or contact: HE Nieburgs, MD email: cancer@banyan.ummed.edu post: Cancer Detection and Prevention University of Massachusetts Medical Center 55 Lake Avenue North Box 20 Worcester, MA 01655 USA tel: (508) 856-1822 fax: (508) 856-1824 From owner-biophysics@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!sdcc12.ucsd.edu!e6501-9!gfischer From: gfischer@e6501-9.ucsd.edu (Gregory Fischer) Newsgroups: bionet.biophysics,fj.sci.physics,sci.physics,sci.physics.electromag,sci.physics.particle Subject: Re: Information on glueballs, quarks, and anti-quarks Date: 8 Jan 1996 18:52:14 GMT Organization: University of California, San Diego Lines: 34 Message-ID: <4crp4u$8p1@sdcc12.ucsd.edu> References: <4c7cnp$gnm@nn.fast.net> NNTP-Posting-Host: e6501-9.ucsd.edu Xref: biosci bionet.biophysics:1563 sci.physics:116899 sci.physics.electromag:7572 sci.physics.particle:5625 In article <4c7cnp$gnm@nn.fast.net> mpb@fast.net writes: >I am a 10th grader taking AP Biology. As part of the circurriculum, I have to complete a research project for The DuPont Challenge. >The DuPont Challenge "is an essay of 700 to 1000 words discussing a scientific development, event, or theory taht has captured >your interest and attention." >The glueball is what I am writing my research project on. > >For research, I an using the Internet, scientific journals (incuding Physical Review Letters), enclylopedias, and other sources. I would >also like to contact the IBM Thomas J. Watson Research Center (where the research was done), but am unsure of how to do that. > >Also, any suggestions as to where to find other resources about glueballs, quarks, or anti-quarks would help me greatly. > >Thanks, > >BG >mpb@fast.net Scientific American might be a good place for you to look. It often has nice, simply wriiten articles that yield a basic understanding of what can be rather difficult concepts. They also provide references that can lead to more in depth discussions. Good luck! Greg -- Greg Fischer | Oh beware the other head of Graduate Student, Materials Science Group | science Arthur, it bites! University of California, San Diego | E-mail: gfischer@ucsd.edu | --The Tick, animated series From owner-biophysics@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!PUBLIC.BTA.NET.CN!wfchen From: wfchen@PUBLIC.BTA.NET.CN (user 3046) Newsgroups: bionet.biophysics Subject: (none) Date: 8 Jan 1996 03:22:05 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 46 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601081119.TAA02125@public.bta.net.cn> NNTP-Posting-Host: net.bio.net mRNA Strucrure Prediction Internet-Service Dear Experts: Please permit me to ask a naive question. We are just probing into the area of structural biology from the field of molecular immunology! We want to know the 2nd especially 3rd mRNA structure from our mRNA sequence. Computer modelling is just enough! The result is very meaningful to us! The OBSTACLE we are facing: our computer is not power enough. ARE THERE any e-mail server on the Internet to do that just as 'PredictProtein@EMBL-Heidelberg.DE'? OR, SHOULD YOU permit me use your mainframe to do this computation (I send you the seq. via e-mail)? I deem it a great honor to receive your early reply. Please reply personally to wfchen@public.bta.net.cn =-=-=-=-=-=-=-=-=-=-=-=-=- MANY THANKS! -=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Simon M. Lin Department of Immunology Phone: +86 10 593 4831 (24 hrs) Beijing Medical University CHINA "Not quite the third world" =-=-=-=-==-=-=-=-=-=-="Computer + Molecular Biology = Discovery !" -=-=-=-= From owner-biophysics@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!usc!howland.reston.ans.net!news.sprintlink.net!news.interserv.net!news2.interserv.net!news From: golem@interserv.com Newsgroups: bionet.biophysics, Subject: about clocks Date: 8 Jan 1996 04:17:48 GMT Organization: InterServ News Service Lines: 4 Message-ID: <4cq5tc$3av@lore.interserv.net> NNTP-Posting-Host: ad45-151.compuserve.com Does anybody know about a group that talks about biological rhythmicity, circadian clocks, melatonin, temporal organization of development etc.? Thank you, Bora Zivkovic, NCSU From owner-biophysics@net.bio.net Mon Jan 08 22:00:00 1996 Path: biosci!bloom-beacon.mit.edu!newsfeed.internetmci.com!in2.uu.net!news.tuwien.ac.at!fbma.tuwien.ac.at!e8627164 From: e8627164@fbma.tuwien.ac.at (Otto Hainzl) Newsgroups: bionet.biophysics, Subject: Re: about clocks Followup-To: bionet.biophysics, Date: 9 Jan 1996 14:10:36 GMT Organization: Vienna University of Technology, Austria Lines: 12 Message-ID: <4ctt0s$2sn@news.tuwien.ac.at> References: <4cq5tc$3av@lore.interserv.net> NNTP-Posting-Host: fbma.tuwien.ac.at X-Newsreader: TIN [version 1.2 PL2] golem@interserv.com wrote: : Does anybody know about a group that talks about biological rhythmicity, circadian clocks, : melatonin, temporal organization of development etc.? This is not what you are looking for but still: There is a German book 'Biologische Uhren' (Biological Clocks) from Spektrum der Wissenschaft (Germans equivalent to Scientific American) It is not the hell of a scientific book but it has a good bibliography. If you are really interested I can find out for you if there is an English Version. Otto From owner-biophysics@net.bio.net Mon Jan 08 22:00:00 1996 Path: biosci!entu.cas.cz!stys From: stys@entu.cas.cz (Dalibor Stys) Newsgroups: bionet.biophysics Subject: POSITION WANTED -FATAL ERROR IN PREVIOUS POSTING Date: 8 Jan 1996 22:15:14 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I deeply appologise, the salary offer should read $200 a month instead a year, of course Dalibor Stys From owner-biophysics@net.bio.net Mon Jan 08 22:00:00 1996 Path: biosci!entu.cas.cz!stys From: stys@entu.cas.cz (Dalibor Stys) Newsgroups: bionet.biophysics Subject: POSITION OPEN Date: 8 Jan 1996 22:05:57 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 66 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear friends, I am constituting a team working on structure-function relationship in membrane proteins and structural basis of interactions in biological membranes at the South Bohemian University in Budweis, Czech Republic. The team will be supported by a team grant from Czech Ministery of Education and the suport will be rather generous, allowing us to equipp the laboratories and to work without material shortage for next five years. The team is constituted in colla boration with Prof. Da-Neng Wang from New York and will cover the experimental range from isolation of membrane proteins and their reconstitution through crystallisation and structure determinantion by X-ray and electrone diffraction towards TRNOE NMR of interacting domains and bound ligands. Initially we will follow two lines - proteins from photosynthetic membranes and membrane receptors related to multidrug resistance but we are open to accept project which the group members bring with themselves in case that they will fit into the general line of the project. There are still 4-5 open positions on the assistant professor level as co-applicants on the grant application for the research group. We particularly welcome with biochemical background, preferentially with partial knowledge of purifiaction of membrane proteins, reconstitution of membrane proteins into vesicles or crystallisation of membrane proteins but there is no strict limitation on it. Interested people will be directing their own partially independent research line and supervising undergraduated and Ph.D. students and teaching maximum 20% (probably less) of their working time. The positions are career-like since the university is bound to integrate the team into its structure after five years. The salaries in the Czech Republic are approximately $200 a year and the living costs are about to 1/4-1/5 of those in the west. The positions are thus open mainly to researchers from former Soviet Union. Interested scientists form the west are very welcome and may be accepted but are encouraged, in their own interest, to apply for an independent source to support their stay. The deadline for the application is end of February 1996, results will be known in the end of June and positions will be available from October 1996. Please send your CV, list of recent publications to Dr. Dalibor Stys, preferably by e-mail on stys@entu.cas.cz or dalibor.stys@plantcell.lu.se or post to Institute of Microbiology, Czech Academy of Science, CZ-379 81 Trebon-Opatovicky mlyn, Czech republic or tel/fax. +42 333 2268, or tel. +42 333 721101 or 721140. I would be happy to answer all questions about the details of this project. PLEASE ACT IMMEDIATELY, SINCE YOU SHOULD BE LISTED ON THE GRANT APPLICATION AND SIGN IT. SINCE I AM NOT SUBSCRIBING MOST OF THE NETWORKS TO WHICH I SENT THE REQUEST, PLEASE, SEND YOUR MAIL DIRECTLY TO ME. Best regards Dr. Dalibor Stys ********************************************************************* Dr. Dalibor Stys Institute of Microbiology Academy of Science of the Czech Republic CZ-379 81 Trebon - Opatovicky mlyn Czech Republic tel. +42 333 721101 or 721140 tel/fax. +42 333 2268 e-mail stys@entu.cas.cz or dalibor.stys@plantcell.lu.se ******************************************************************** From owner-biophysics@net.bio.net Mon Jan 08 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!vixen.cso.uiuc.edu!howland.reston.ans.net!tank.news.pipex.net!pipex!demon!mail2news.demon.co.uk![192.129.2.42] From: "Dr. Ulrich Schroeder" Newsgroups: bionet.biophysics Subject: Know company? Date: Tue, 9 Jan 96 17:21:18 EST Lines: 19 Message-ID: <73543.schroede@jupiter.ifn-magdeburg.de> X-NNTP-Posting-Host: [192.129.2.42] X-Minuet-Version: Minuet1.0_Beta_14.7 X-Popmail-Charset: English X-Mail2News-Path: dfnserv1.URZ.Uni-Magdeburg.DE!jupiter.ifn-magdeburg.de![192.129.2.42] I am looking for the address of Merck, Sharp & Dohme. Does anybody know the address, e-mail, fax number or telephone number? Thanks in advance for your efforts. Best regards Ulli ------------------------------- Dr. Ulrich Schroeder BL-Institut fuer Neurobiologie Dept. of Neurophysiology PO.Box 1860 39008 Magdeburg Germany Tel:(**49)/0391/6263431 Fax:(**49)/0391/6263438 e-mail: schroede@jupiter.ifn-magdeburg.de From owner-biophysics@net.bio.net Mon Jan 08 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!news.cais.net!news.his.com!news From: Dietmar Tietz Newsgroups: bionet.biophysics,bionet.diagnostics,bionet.genome.chromosomes Subject: --> CALL FOR PAPERS <-- Date: 9 Jan 1996 21:03:43 GMT Organization: Heller Information Services, Inc. Lines: 35 Message-ID: <4cul7f$gt3@news2.his.com> NNTP-Posting-Host: djt.his.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.12(Macintosh; I; 68K) To: djt@his.com X-URL: newsrc://news.his.com/ Xref: biosci bionet.biophysics:1572 bionet.diagnostics:528 bionet.genome.chromosomes:995 Dear Researchers: I am writing to you as the Editor of a book on Nucleic Acid Electrophoresis which is to be issued by Springer Verlag (Berlin, Heidelberg, New York), one of the leading scientific publishers. This book is intended to be part of the Springer Lab Manual Series which takes a practical approach and addresses a wide spectrum of readers extending from the college student to the senior investigator. The objective of this series is to communicate hands-on experience and to provide practical hints including easy-to-follow methods. At a time when research papers have become so short that they can barely describe the methods in sufficient detail, this lab manual will be a good opportunity to transmit to the scientific community a broader spectrum of your unpublished observations and valuable experience. Please contact me if you are interested in contributing a chapter. Our aim is to cover all aspects of electrophoresis. At this time, we are particularly interested in applications related to DNA sequencing and capillary electrophoresis. Thank you for your interest. Looking forward to hearing from you I remain, Sincerely yours, Dietmar Tietz, Ph.D. Fax: USA-(301)-681-7002 Email: djt@his.com or TVJ@CU.NIH.GOV WWW: http://www.his.com/~djt/ From owner-biophysics@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!news.uoregon.edu!news.bc.net!news.sfu.ca!beaufort!hunterf From: Greg Hunter Newsgroups: bionet.biophysics Subject: Re: Area of a single phospholipid Date: Tue, 9 Jan 1996 15:37:04 -0800 Organization: Simon Fraser University Lines: 36 Message-ID: References: <4cjelt$b4g@mserv1.dl.ac.uk> NNTP-Posting-Host: beaufort.sfu.ca Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: hunterf@beaufort In-Reply-To: <4cjelt$b4g@mserv1.dl.ac.uk> On 5 Jan 1996, Paul. S. Brookes. wrote: > I'm doing calculations that require knowledge of the area occupied by a > phospholipid in a bilayer. > > This concept can be interpreted in 2 ways:- > <> It's the surface area occupied by a single PL head group when viewed > looking down onto the plane of a lipid bilayer, in which case to get the > area of bilayer from a known number of lipid molecules, one has to multiply > single PL area by PL number, then halve the answer (equivalent to folding > the monolayer in two). > > or <> It is the amount of bilayer area made by a single PL (a silly > concept if ever there was one), obviously having been calculated from an > area of bilayer divided by the number of PL molecules present, without > correcting for lamellarity. > > The literature gives a range of values:- > (snip) > Please, if you know which figure should be used, whether they refer to > monolayer/bilayer areas, or have any figures of your own, I'd be most > grateful for further information, as having calculations out by a factor of > 2 is just a little bit outside my error limits! > The area of a phospholipid depends on the headgroup attached to it. Phosphatidylcholines have a greater area than, say, phosphatidylethanolamines. A good starting reference is the CRC Handbook of Lipid Bilayers, CRC Press, 1990. Hope this helps ----------------------------------------- Xanadu conifers crush zeolite quietly in the mist. Greg Hunter hunterf@sfu.ca From owner-biophysics@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!fdn.fr!jussieu.fr!univ-lyon1.fr!ws41.cnusc.fr!news!usenet From: Denis@CRBM.cnrs-mop.fr (Denis Pugnere) Newsgroups: bionet.neuroscience,bionet.biophysics,bionet.cardiovascular Subject: Appel a voter : fr.bio.canauxioniques Date: 10 Jan 1996 16:43:29 GMT Organization: CRBM Lines: 55 Message-ID: <4d0qbh$3ab@quepassa.lirmm.fr> NNTP-Posting-Host: 193.49.189.90 Mime-Version: 1.0 Content-Type: Text/Plain; charset=ISO-8859-1 X-Newsreader: WinVN 0.99.5 Xref: biosci bionet.neuroscience:12025 bionet.biophysics:1574 Date de fin du vote : Jeudi 18 janvier a 23h59m (heure Francaise metropolitaine). Cet article est un appel a voter pour ou contre la creation d'un usenet-group non modere fr.bio.canauxioniques. Cet appel a ete cross-postee dans les usenet-groups suivants : fr.bio.general, fr.usenet.groups, fr.announce.newgroups, bionet.neuroscience, bionet.cellbiol, bionet.biophysics, bionet.cardiovascular, bionet.announce Comment voter : Si vous votez POUR la creation du groupe fr.bio.canauxioniques : envoyez un e-mail a l'adresse : oui@altair.crbm.cnrs-mop.fr Si vous votez CONTRE la creation du groupe fr.bio.canauxioniques : envoyez un e-mail a l'adresse : non@altair.crbm.cnrs-mop.fr Le corps du message n'a aucune importance. Il vous sera renvoye un accuse de reception pour valider le vote. Les doublons et les votes anonymes seront elimines. Date de fin du vote : Jeudi 18 janvier a 23h59m (heure Francaise metropolitaine). DEFINITION DU NEWSGROUP ----------------------- Nom : fr.bio.canauxioniques Statut : Non modere Objet : Groupe de discussion traitant de la recherche sur les canaux ioniques et des techniques associees. Description : Le groupe fr.bio.canauxioniques est destine a etre un lieu d'echange theorique, technique et pratique pour les chercheurs et etudiants interesses par les divers aspects de l'etude des canaux ioniques au sens large (canaux, echangeurs, recepteurs et exitabilite membranaire en general) ; c'est a dire essentiellement l'electrophysiologie, la biologie cellulaire et moleculaire et la biochimie. Ce groupe servira aussi a l'annonce de congres, seminaires, theses et demande de post-doc pouvant interesser les chercheurs travaillant sur cette thematique. --- Denis Pugnere (Denis@CRBM.cnrs-mop.fr) Pierre Charnet (charnet@kaa.CRBM.cnrs-mop.fr) From owner-biophysics@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!UQTR.UQuebec.ca!etu01088 From: etu01088@UQTR.UQuebec.ca (Martin Maltais) Newsgroups: bionet.biophysics Subject: Re: education Date: 10 Jan 1996 11:02:15 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <01HZDLM7FOHU000XU7@buphyc.bu.edu> NNTP-Posting-Host: net.bio.net Dear Mr. Chasan, I'm studying biophysics at l'universite du Quebec a Trois-Rivieres at a B.A. level. In Quebec, it is the only place that we can studie it at that level because biophysic use to be a master or Ph degre. I have a strong feeling too that there is much to be done in the area of biophysics education. The knowledge that is teaching me right now is so far away from biophysics that I'm sking me often if I'm in the right place. There is more to be done at a basic level of teaching biophysics. I don't know if I am clear, but I just want to tell you that I agree with your feeling and from my 20 years old experience I think that it's a fact that there is more to do there. Martin Maltais etudiant en biophysique U.Q.T.R. Trois-Rivieres From owner-biophysics@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!bloom-beacon.mit.edu!senator-bedfellow.mit.edu!takahara From: takahara@athena.mit.edu (Patricia M Takahara) Newsgroups: bionet.xtallography,bionet.biophysics Subject: Postdoctoral Position at Harvard Medical School and MIT Date: 10 Jan 1996 23:41:06 GMT Organization: Massachvsetts Institvte of Technology Lines: 40 Message-ID: <4d1iqi$fkc@senator-bedfellow.MIT.EDU> NNTP-Posting-Host: alfredo.mit.edu Xref: biosci bionet.xtallography:2300 bionet.biophysics:1578 Postdoctoral Position Protein Crystallography & Bioinorganic Chemistry A POSTDOCTORAL POSITION is available in PROTEIN CRYSTALLOGRAPHY and BIOINORGANIC CHEMISTRY in a collaborative project between the laboratories of Christin A. Frederick (Harvard Medical School - Dana-Farber Cancer Institute, Department of Biochemistry and Molecular Pharmacology) and Stephen J. Lippard (Massachusetts Institute of Technology, Department of Chemistry). Applicants should have a Ph.D. in chemistry, biochemistry, or biophysics and research experience in macromolecular crystallography. The successful candidate will study the structure and aspects of the mechanism of the proteins comprising the soluble, bacterial methane monooxygenase system by using protein crystallography. Two Mar Research imaging plate systems, one multiwire system, and full computing facilities are available for the research. Data will also be collected at synchrotron radiation sources. The deadline for applications is Feb. 1, 1996. Applicants should submit a letter including a description of research experience, a CV, and the names, addresses and phone numbers of three references to: Professor Christin A. Frederick Harvard Medical School - Dana-Farber Cancer Institute Dept. of Biochemistry and Molecular Pharmacology 44 Binney St. Room Dana 1040 Boston, MA 02115 and Professor Stephen J. Lippard Massachusetts Institute of Technology Dept. of Chemistry 77 Massachusetts Avenue Room 18-590 Cambridge, MA 02139 The position is available immediately. From owner-biophysics@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!lll-winken.llnl.gov!usenet From: Chris Barry Newsgroups: bionet.neuroscience,bionet.biophysics,bionet.cardiovascular Subject: Re: Appel a voter : fr.bio.canauxioniques Date: Wed, 10 Jan 1996 14:43:22 -0800 Organization: Lawrence Livermore National Laboratory Lines: 2 Message-ID: <30F4410A.1A23D8B5@ucdavis.edu> References: <4d0qbh$3ab@quepassa.lirmm.fr> NNTP-Posting-Host: mackiller.llnl.gov Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b4 (X11; I; Linux 1.1.72 i486) Xref: biosci bionet.neuroscience:12027 bionet.biophysics:1577 Why do you need a vote? If you want the group, why don't you just form the group? From owner-biophysics@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!vixen.cso.uiuc.edu!howland.reston.ans.net!nntp.coast.net!swidir.switch.ch!swsbe6.switch.ch!scsing.switch.ch!news.belwue.de!newsserv.zdv.uni-tuebingen.de!hno01.hno.medizin.uni-tuebingen.de!user From: ulix@uni-tuebingen.de (Ulrich Rexhausen) Newsgroups: bionet.biophysics Subject: Micropipette Positioning. Date: 10 Jan 1996 19:28:08 GMT Organization: HNO-Universitätsklinik Tübingen Lines: 64 Message-ID: NNTP-Posting-Host: hno01.hno.medizin.uni-tuebingen.de Hallo, Iam working at a B.Sc Thesis in "Sensorical Biophysics" about AUTOMATICAL PRECISION LASER POSITIONING OF MICROPIPETTES FOR PATCH CLAMP SETUPS in Elektrophysiology. I would be happy if there are people who are interested and have experience or a good idea about that. Maybe there is someone working on the same thing, because all "Patch Clampers" and other people dealing with micropositioning should be confrontated with the same problem. First I explain the technique and then the idea for a Micropipette Positioning System: The Patch Clamp technique is an important cell physiological method that allows the properties and dynamics of single ion channels in the membrane of biological cells to be studied (in 1991 Sakman and Neher won the Nobel Price in medicine for the development of this technique). Since the pipette tip is fragile optical methods for the positioning are favorized.The problem is, that the length of the pipettes vary. This makes the positioning of the pipettes more complicated. Because of that it is important to determine the position of the pipette tip as a fixed reference point, so that it is possible to position the pipette automatically by use of the manipulator in front of the cell. The idea to get a fixed reference point for the tip of the pipette is: // //<== pipette // lens // _______ | // || | | o // o || | ------- | o / o || | ------- | o o || laser | ------- | x || <== array of Photodetectors | ------- | o o || | ------- | o | o || _______| | o | o || | | || | | | laser fokus A diode laser, a lens to focus its beam, and a circular array of photodetectors are fixed to the microscope stage. The micropipette inside the focused laser beam produces a characteristic shadowing which can be detected by the array of photodetectors. Quantifyinig the distortion of the illumination of the detector array as a function of x,y, and z enables the position of the pipette tip to the focus of the laser beam, which is the reference point. Another method could be the use of a CCD-camera. The pipette is imaged on a chip and the darkening of the pixels at the chip are read by means of computer. Now you know all thoughts I have aboute this. So if you are interessted or experienced in AUTOMATICAL PRESICION LASER POSITIONING OF MICROPIPETTES it would be great to hear from you. yours Detlef Repplinger (email : detlef.repplinger@student.uni-tuebingen.de) From owner-biophysics@net.bio.net Wed Jan 10 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: sproutsrad@aol.com (SproutsRad) Newsgroups: bionet.biophysics Subject: information theory Date: 11 Jan 1996 06:09:22 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 38 Sender: root@newsbf02.news.aol.com Message-ID: <4d2r52$p29@newsbf02.news.aol.com> Reply-To: sproutsrad@aol.com (SproutsRad) NNTP-Posting-Host: newsbf02.mail.aol.com This homely argument is an effort to understand "information" in relation to the organization of energy in biological processes (and in general). The broad notion is that "information" functions as a constraint on the possible paths of energy and that "concerted action" signals an informational process. Biological process is largely driven by the influx of solar energy which is delivered in units of about one electron volt, the amount of energy given up by a photon in photosynthesis. Biological process is able to bring these small units of energy to a high degree of like mindedness. As a simple example, consider that a pitched baseball weighing five ounces and traveling at 90 mile/hr has a kinetic energy of 7.8529 * 10^ 22 electron volts. Can we say that this concerted action signals an informational process? Well, continuing in the baseball theme and trying to look at it in terms of information theory, consider that a baseball emanating "randomly" from the pitcher's mound has one chance in 5750 of hitting a 4 sq ft strike zone 60.5 feet away (considering only the above ground hemisphere). If our symbol set has 5750 symbols and we designate a strike by the symbol "s", then a pitcher who consistently throws strikes is a source (P) generating the signal "ssssssssss . . . . .". By Shannon's entropy measure the source (P) is fully redundant and therefor non-informational. While this is entirely true from the view point of a communications engineer (outside the black box), it does not at all reflect the internal organization of energy effected by biological process culminating in the efforts of the pitcher. Can you offer some perspective on this? Is it a manufactured dilemma? I would appreciate your thoughts. . Don Foster (SproutsRad@aol.com) "Technology is the answer, but what was the question?" Amory Lovins From owner-biophysics@net.bio.net Wed Jan 10 22:00:00 1996 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!newsfeed.internetmci.com!gatech!sdd.hp.com!usc!news.cerf.net!newsserver.sdsc.edu!news.tc.cornell.edu!newsstand.cit.cornell.edu!usenet From: Tom Chou Newsgroups: bionet.biophysics Subject: Polymer/Protein-Charge Interactions? Date: 10 Jan 1996 17:30:16 GMT Organization: LASSP, Cornell Univ. Lines: 12 Sender: tc42@cornell.edu (Verified) Message-ID: <4d0t38$b4o@newsstand.cit.cornell.edu> NNTP-Posting-Host: rahab.msc.cornell.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; AIX 2) X-URL: news:bionet.biophysics Hi, I'm thinking about the electrostatics of proteins which bind/attach to membranes. Has the electrostatics, ala Poisson-Boltzmann, been studied? I'd be appreciative if you can point me in the right direction, Thanks, Tom From owner-biophysics@net.bio.net Wed Jan 10 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!EU.net!peer-news.britain.eu.net!keele!daresbury!not-for-mail From: "Doering.Olaf" Newsgroups: bionet.biophysics Subject: Re: Micropipette Positioning. Date: 11 Jan 1996 12:40:50 -0000 Lines: 27 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4d30gi$14k@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk Dear Detlef reading your mail I was thinking about another idea of how to position a pipette (although I did not actually try to do this): Use the micropipette as an optical fibre. Send the light (laser or other light of non-disturbing wavelength) into the pipette at the blunt end (you will have to re--design your pipette holder). The light will emerge from the pipette at the tip. Focus the tip with a microscope equipped with a stepper-motor for focusing, a beam-splitter and your CCD-array attached to it. By minimizing the diameter of the light spot of the pipette tip on the CCD you should be able to get information about the position of the tip in the 3rd dimension. This should work with both, 'normal' or inverted microscopes. If you are successful with your work please let me know about your results. Greetings Olaf O. Doering Univ. Hamburg Inst. Botany Ohnhorststr. 18 D-22609 Hamburg, FRG Phone: +49-40-82282-348 FAX: +49-40-82282-254 From owner-biophysics@net.bio.net Thu Jan 11 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!sgigate.sgi.com!sdd.hp.com!hamblin.math.byu.edu!acs2.byu.edu!news.cuny.edu!news From: batlle Newsgroups: bionet.biophysics Subject: Re: Acetylcholine structure? Date: 12 Jan 1996 01:52:03 GMT Organization: City University of New York/University Computer Center Lines: 11 Message-ID: <4d4es3$uf9@news.cuny.edu> References: <4chakl$of@abel.cc.sunysb.edu> NNTP-Posting-Host: 146.203.3.147 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: culli@delbruck X-URL: news:4chakl$of@abel.cc.sunysb.edu Hi, My email is foloppe@msvax.mssm.edu, and not batlle... What I would do is to search the cambridge data base for the molecules you need. Are you modelling the acethylcholine receptor ? Sincerely, Nicolas Foloppe From owner-biophysics@net.bio.net Thu Jan 11 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!gatech!newsfeed.internetmci.com!in1.uu.net!fdn.fr!jussieu.fr!citi2.fr!news From: tamisier@bisance.citi2.fr (Luc Tamisier) Newsgroups: bionet.biophysics Subject: morphine receptors Followup-To: sci.med.physics Date: 12 Jan 1996 13:18:06 GMT Organization: GRPB Lines: 11 Message-ID: <4d5n2e$78c@bisance.citi2.fr> NNTP-Posting-Host: u40013.citi2.fr Mime-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII X-Newsreader: WinVN 0.99.7 Hi all, I am looking for information on morphine receptors. Could someone tell me if they are readily extractable? If so, where can we get (buy, ...) them? Thank you very very much. -- Luc Tamisier GRPB-Universite Rene Descartes 45, rue des St Peres, 75270 Paris cedex06, France Tel: 42 86 21 30 Fax: 42 86 20 85 http://u40024.citi2.fr/grpb/ From owner-biophysics@net.bio.net Thu Jan 11 22:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!ames!uhog.mit.edu!news.kei.com!newsfeed.internetmci.com!in1.uu.net!sunrise.gv.ssi1.com!oronet!news From: tessien@oro.net (Ross Tessien) Newsgroups: bionet.biophysics,fj.sci.physics,sci.physics,sci.physics.electromag,sci.physics.particle Subject: Re: Information on glueballs, quarks, and anti-quarks Date: 12 Jan 1996 05:03:34 GMT Organization: Impulse Engineering, Inc. Lines: 86 Message-ID: <4d4q36$v26@hg.oro.net> References: <4c7cnp$gnm@nn.fast.net> <4crp4u$8p1@sdcc12.ucsd.edu> <4cs34s$6la@guitar.ucr.edu> NNTP-Posting-Host: tessien.oro.net Mime-Version: 1.0 X-Newsreader: WinVN 0.93.14 Xref: biosci bionet.biophysics:1583 sci.physics:117577 sci.physics.electromag:7619 sci.physics.particle:5714 In article <4cs34s$6la@guitar.ucr.edu>, baez@guitar.ucr.edu says... > >>In article <4c7cnp$gnm@nn.fast.net> mpb@fast.net writes: > >>>The glueball is what I am writing my research project on. > >Why in the world would you ask a question about glueballs on a >biophysics newsgroup? Oh well... > >Here's some stuff from week 68 of my series, This Week's Finds in >Mathematical Physics: > >4) Frank Close, Are glueballs and hybrids found?, preprint available as >hep-ph/9509245 style file sprocl.sty required (available from hep-ph). >To appear in Proceedings of Hadron95. > >J. Sexton, A. Vaccarino, D. Weingarten, Numerical evidence for the >observation of a scalar glueball, preprint available as hep-lat/9510022. > >Thanks go to Greg Kilcup for bringing these to my attention. Have they >found a glueball??? That would be really exciting. What's a glueball, >you ask? Well, quantum chromodynamics, our best theory of the strong >force, says that that the strong force is carried by particles called >"gluons". Like electromagnetism, the strong force is a gauge field, but >it's a nonabelian gauge field, so the gluons themselves have charge, or >"color". Thus they interact in a nonlinear way. This is what lets them >bind together quarks in such a tight way. But perhaps, in addition to >pairs of quarks and antiquarks held together by gluons --- i.e., mesons >--- and triples of quarks held together by gluons --- i.e., baryons --- >there could be short-lived assemblages consisting entirely of gluons, >held together by their self-interactions. These are called glueballs, but >we don't know if these exist. > >However, to my surprise, it turns out that there are now some candidates >out there! The first paper suggests that the f_0(1500), a neutral >spin-0 particle with mass around 1500 MeV, is a glueball. The second >paper argues instead that this is basically a quark-antiquark pair (made >of a strange quark and a strange antiquark... where "strange" is the >technical name for one of the 6 quarks!). It presents evidence from a >numerical simulation and argues that the "theta" or f_J(1710), a >spin-zero particle with mass 1710 MeV, is a glueball. > >It's tough to do nonperturbative computations in nonlinear gauge field >theories --- basically one needs to approximately compute a path >integral, using Monte Carlo technique, approximating spacetime by a >lattice (in this case, a 16 x 16 x 16 x 24 lattice). Computing the >properties of a glueball and matching it with an experimentally observed >particle would be a marvelous confirmation of quantum chromodynamics. >In addition, I find there to be something charming about the idea that >in a nonabelian gauge theory we could have a particle made simply of the >gauge field itself. > >------ >If you don't know how to get papers from hep-ph or hep-lat, >send email with subject header > >help > >to hep-ph@xxx.lanl.gov and hep-lat@xxx.lanl.gov. > > John or other particle physicists out there: I have been trying to come to understand some of the ways that sub atomic matter might possibly be constructed. To this end, I am working with geometric models. I know the odds of finding something are low, but this is something I want to do and could use a little help. Under normal circumstances, a positron and an electron will approach and anhialate each other. I was wondering if there have been any suppositions that perhaps quarks and/or gluons (and therefore glueballs) might be composite particles constructed from a large number of electrons and positrons that have come together as a group and are oscillating 180 degrees out of phase with one another. This would be like a bunch of grapes, I suppose, where every other grape is either a positron or an electron where each is in resonance in a spherical, radial, manner. I am exploring large groups of e/p in organizations like those of C60 bucky balls, etc. Any Help? Thanks, Ross From owner-biophysics@net.bio.net Thu Jan 11 22:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!news.dacom.co.kr!news.uoregon.edu!news.rediris.es!power.ci.uv.es!news.ua.es!usenet From: Etelvina Andreu Sanchez Newsgroups: bionet.biophysics Subject: Spikes analysis Date: 12 Jan 1996 19:05:44 GMT Organization: Universidad de Alicante Lines: 12 Message-ID: <4d6be8$d3a@tabarca.cpd.ua.es> NNTP-Posting-Host: juanvi.fisi.ua.es Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; SunOS 5.3 sun4m) X-URL: news:bionet.biophysics I would like to know if there is any software ( freeware, shareware or commercial) to analyze action potential series belonging to different populations. A programm that would distingish "different " spikes taking into account amplitude, duration, depolarization velocity,..etc. Thanks in advance for your kind help E. Andreu -- Etelvina Andreu Sanchez Grupo de Sistemas Neurales.Dpto. Fisiologia & Inst. Neurociencias Universidad de Alicante email: eandreu@juanvi.fisi.ua.es From owner-biophysics@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!ralph.vnet.net!icagen From: castle@icagen.com (Neil A. Castle) Newsgroups: bionet.jobs,bionet.biophysics,sci.research.careers,triangle.jobs,misc.jobs.offered Subject: Jobs: Chemistry, Electrophysiology, Molecular and Cellular Biology, Pharmacology Date: Mon, 15 Jan 96 04:41:55 GMT Organization: ICAgen Lines: 54 Message-ID: <4dcljj$26u@ralph.vnet.net> NNTP-Posting-Host: icagen.com X-Newsreader: News Xpress Version 1.0 Beta #4 Xref: biosci bionet.biophysics:1588 sci.research.careers:8302 misc.jobs.offered:522168 ICAgen, Inc., a RTP-based biopharmaceutical company focused on ion channel drug discovery is expanding its biology team and initiating its chemistry group. · Scientists with the following qualifications are being immediately hired. BIOLOGY Electrophysiology Ph.D. Electrophysiologists at ICAgen lead our biology team using biophysical, molecular and computational approaches in the study of ion channel modulation. Successful candidates must be experienced in a wide range of electrophysiological methods. Molecular Biology Ph.D. As a scientist in this group, you will take a leading role in exploring ion channel subtypes involved in disease. You must have experience in cloning, expression, mutagenesis and tissue distribution studies. Cell Biology/Pharmacology 2 positions - Ph.D., MS/BS You will be involved in all phases of ion channel drug discovery, including the development and completion of high-throughput screening assays. Experience in assay development is required. CHEMISTRY 3 positions - Ph.D. ICAgen is recruiting a core team of chemists to initiate its organic synthesis group. Successful candidates with be organic chemists with either - 3-5 years of medicinal chemistry experience, plus knowledge of molecular simulation methods and combinatorial chemistry, or - postdoctoral experience encompassing either molecular simulation methods or combinatorial chemistry techniques, or - direct experience in molecular diversity/combinatorial chemistry (both solution or solid phase synthesis). ICAgen provides competitive salaries, a full benefits package and equity in this privately held Company. ICAgen is an equal opportunity employer. Please send resumes, clearly stating specific position for which you are applying to ICAgen, Inc., Human Resources P.O. Box 14487, RTP, N.C. 27709 Alternatively, E-mail resume (in ascii format)to; castle@icagen.com From owner-biophysics@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!sc106.krasnoyarsk.su!sad From: sad@sc106.krasnoyarsk.su ("Michail G.Sadovsky") Newsgroups: bionet.biophysics Subject: (none) Date: 15 Jan 1996 00:44:44 -0800 Organization: The Krasnoyarsk Experimental SchoolUnivers Lines: 53 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net To: SproutsRad, (sproutsrad@aol.com (SproutsRad)) In the message <4d2r52$p29@newsbf02.news.aol.com> the author wrote: >This homely argument is an effort to understand "information" in relation >to the organization of energy in biological processes (and in general). > ...... The point is that the energy flux ABSOLUTELY can not be considered as an information. Your remark on the redundancy of the flux >If our symbol set has 5750 symbols and we designate a strike by the symbol >"s", then a pitcher who consistently throws strikes is a source (P) >generating the signal "ssssssssss . . . . .". By Shannon's entropy >measure the source (P) is fully redundant and therefor non-informational. >While this is entirely true from the view point of a communications >engineer (outside the black box), it does not at all reflect the internal >organization of energy effected by biological process culminating in the >efforts of the pitcher. is OK, but it does not mean that the energy could be consumed as it is. To my opinion, You meet the same mistake came from a misrepresentation of an information content (i.e. its amount) and a meaning (i.e. its value). The matter is that there is no relation between the former and the latter. Two messages: "John loves Mary" and "Mr Eltzin announced a war to Mr Clinton" actually contain the same quantity of the information, while there meaning (even from our personal point of view - concerning the impact on our fortunes) is drastically different. Another rough analogy is that one may consume bread and water only, and still will be healthy (or, at least, alive), while the diet is not tasty at all (low information content!). You might want to read more on this topic (I mean a relation between amount and value of the biological information in our paper: N.N.Bugaenko, A.N.Gorban, M.G.Sadovsky On the information content of nucleotide sequences /Mol.Biologiya (Molecular Biology), 1996 - to be appeared in Russian journal "Molekulyarnaya Biologiya". But this journal is SIMULTANEOUSLY translated into English (no delay in publication) by Pergamon (or, maybe, Kluwer - I don't know exactly) and published as "Molecular Biology". Best regards, Michael G.Sadovsky, PhD Institute of Biophysics of Siberian Division of Russian Academy of Sciences; 660036 Russia, Krasnoyarsk, Siberia tel. 7-(3912)-494101 (voice) fax: 7-(3912)-433400 e-mail: sad@sc106.krasnoyarsk.su From owner-biophysics@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!gimbel.com!ocuzzani From: ocuzzani@gimbel.com ("Dr. Cuzzani") Newsgroups: bionet.biophysics Subject: Unsubscribe Date: 15 Jan 1996 09:39:42 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601151739.JAA00969@net.bio.net> NNTP-Posting-Host: net.bio.net Unsubscribe biophys Oscar Cuzzani, MD, DSc Gimbel Eye Centre Suite #450- 4935 40th. Ave. NW Calgary, AB Canada T3A 2N1 Tel: 403-286-6969 Fax: 403-286-2943 From owner-biophysics@net.bio.net Mon Jan 15 22:00:00 1996 Path: biosci!BUHO.DPI.UDEC.CL!mbunster From: mbunster@BUHO.DPI.UDEC.CL (Marta Bunster B) Newsgroups: bionet.biophysics Subject: (none) Date: 16 Jan 1996 03:34:07 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <9601161427.AA18708@buho.dpi.udec.cl> NNTP-Posting-Host: net.bio.net test From owner-biophysics@net.bio.net Mon Jan 15 22:00:00 1996 Path: biosci!ICT.SLD.CU!NORMANDO From: NORMANDO@ICT.SLD.CU (Normando Iznaga) Newsgroups: bionet.biophysics Subject: (none) Date: 15 Jan 1996 21:54:04 -0800 Organization: Centro de Inmunologia Molecular Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: <9601130103.AA00298@infomed> NNTP-Posting-Host: net.bio.net Hi netters, We would like you to help us. We are looking for a supplier of W- 188/Re-188 generators. These are a kind of generators for radionuclides used to eluted renium for labeling monoclonal antibodies for radioimmunodiagnosis and radioimmunotherapy purposes. Thanks in advance, Best Regards, !*************************************! ! M. Sc. Normando Iznaga Escobar ! ! Center of Molecular Immunology ! ! Division of Antibody Engineering ! ! PO BOX 16040, Havana, 11 600, Cuba ! ! ! ! Fax Number : (53 7) 33 50 49 ! ! (53 7) 33 35 09 ! ! Phone : (53 7) 21 68 11 ! ! (53 7) 21 79 33 ! ! e-mail : normando@ict.sld.cu ! !*************************************! From owner-biophysics@net.bio.net Mon Jan 15 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!gatech!newsfeed.internetmci.com!in1.uu.net!newsfeed.ACO.net!fuw.edu.pl!news.nask.org.pl!news.man.lodz.pl!ci.pwr.wroc.pl!news From: Rafal Chmielewski Newsgroups: bionet.biophysics Subject: Scholarship wanted ! Date: Mon, 15 Jan 1996 14:51:59 -0100 Organization: Dept. of Crystallography Lines: 9 Message-ID: <30FA781F.446B@indigo2.chem.uni.wroc.pl> NNTP-Posting-Host: indigo2.chem.uni.wroc.pl Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b4 (X11; I; IRIX 5.3 IP22) Hi everybody, I'm new on this group but I want to ask You for help. Could anyone let me know if tere is ANY foud or organization tht gives scholarships fo Ph.D studing in States? If so, please e-mail me. Thanx -- Rafal Chmielewski rat@indigo2.chem.uni.wroc.pl From owner-biophysics@net.bio.net Mon Jan 15 22:00:00 1996 Path: biosci!POP.UNIV-LILLE1.FR!vergoten From: vergoten@POP.UNIV-LILLE1.FR (Gerard Vergoten) Newsgroups: bionet.biophysics Subject: Re: NATO-ASI on Biomolecular structure and dynamics"NATO Advanced Study Institute" Date: 16 Jan 1996 05:27:08 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <9601161322.AA11250@omega.univ-lille1.fr> NNTP-Posting-Host: net.bio.net Dear Rouzina, Sometimes ago, you asked for information concerning the NATO ASI ` Biomolecular structure and dynamics` to be held in Loutraki (Greece) from may 27 till june 6 1996. Please let me know if you intend to apply. If yes, you can send your application by e-mail Sincerly, G. VERGOTEN From owner-biophysics@net.bio.net Mon Jan 15 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!EU.net!Portugal.EU.net!news.rccn.net!scsing.switch.ch!rzunews.unizh.ch!toukie From: toukie@zui.unizh.ch (Hr Dr. S. Shapiro) Newsgroups: bionet.biophysics Subject: Ellipsometry of biofilms Date: 16 Jan 1996 13:34:46 GMT Organization: University of Zurich, Switzerland Lines: 20 Message-ID: <4dg9hm$dsu@rzunews.unizh.ch> NNTP-Posting-Host: rzurs3.unizh.ch X-Newsreader: TIN [version 1.2 PL2] Dear Colleagues; I require information relatng to the use of ellipsometry for the quanti- fication of biomass accumulating as a biofilm on a thin surface. If anyone has had any experience with this technique, or knows of references describing this methodology, I would be grateful if you would contact me. Sincerely, (Dr.) S. Shapiro Inst. f. orale Mikrobiol. u. allg. Immunol. Zent. f. Zahn-, Mund- u. Kieferheilkd. der Univ. ZH Plattenstr. 11 Postfach CH-8028 ZH 7 Internet: toukie@zui.unizh.ch FAXnr: ( ... + 1) 261'56'83 From owner-biophysics@net.bio.net Tue Jan 16 22:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!news.dacom.co.kr!xpat.postech.ac.kr!galaxy.postech.ac.kr!ppjun From: ppjun@galaxy.postech.ac.kr (Park Byung Jun) Newsgroups: bionet.biophysics Subject: Experimental Data Wanted: Protein(Polymer) Translocation Date: 17 Jan 1996 02:57:30 GMT Organization: POSTECH, Pohang, Korea Lines: 32 Message-ID: <4dhoiq$97k@xpat.postech.ac.kr> NNTP-Posting-Host: galaxy.postech.ac.kr X-Newsreader: TIN [version 1.2 PL1] Dear everybody: I'm now studying the model of polymer translocation through a pore(channel) in a membrane on the basis of theoretical physics. Especially, the translocation rate or the average translocation time was calculated as a function of molecular weight and possible driving force such as chemical potential difference, chaperonin binding, curvature of membrane, etc. I'm about to publish a paper, and wish to incorporate the experimental data if possible. Is there anybody who has the information about the experimental fact related to this study ? I'll wait for your responses not in this board but only via e-mail. Thanks in advance. ----------------------------------------------------------------- P. J. Park Dept. of Physics, Pohang Univ. of Sci. & Tech. San 31, Hyojadong, Pohang, 790-784, Korea Tel: 082-0562-279-5517 Fax: 082-0562-279-3099 email: ppjun@galaxy.postech.ac.kr ----------------------------------------------------------------- From owner-biophysics@net.bio.net Tue Jan 16 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.biophysics Subject: BIOSCI miniFAQ, ver. 14-DEC-95 Date: 17 Jan 1996 02:00:25 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 199 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601171000.CAA06825@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 14-DEC-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index in addition to the master index for the entire set. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-biophysics@net.bio.net Wed Jan 17 22:00:00 1996 Path: biosci!rutgers!gatech!newsjunkie.ans.net!news-m01.ny.us.ibm.net!usenet From: ralcon@ibm.net Newsgroups: bionet.biophysics Subject: Spectra of common biological stains Date: 18 Jan 1996 18:28:18 GMT Lines: 6 Message-ID: <4dm3g2$3auo@news-s01.ny.us.ibm.net> Reply-To: ralcon@ibm.net NNTP-Posting-Host: slip5-81.fl.us.ibm.net X-Newsreader: IBM NewsReader/2 v1.2.5 Hi all. I'd like to know the UV-Vis-Near IR absorbtion and emission spectra for common biological stains (eg EthBr bound to DNA, Coomassie Blue, etc). I cant find a reference book that gives good info, I need spectra, not just Ex-Max and Em-Max. Does anyone know a good reference book. Thanks RALCON@ibm.net From owner-biophysics@net.bio.net Wed Jan 17 22:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!moonbeam.aecom.yu.edu!usenet From: poptest Newsgroups: bionet.biophysics Subject: Re: Polymer/Protein-Charge Interactions? Date: 18 Jan 1996 16:44:10 GMT Organization: AECOM Lines: 2 Message-ID: <4dltcq$n62@moonbeam.aecom.yu.edu> References: <4d0t38$b4o@newsstand.cit.cornell.edu> NNTP-Posting-Host: b14showpc.aecom.yu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Did anybody reply to your question? From owner-biophysics@net.bio.net Wed Jan 17 22:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!uwm.edu!msunews!netnews.upenn.edu!pobox.upenn.edu!gold From: gold@pobox.upenn.edu (Geoffrey H. Gold) Newsgroups: bionet.neuroscience,bionet.biophysics,sci.chem,sci.chem.labware,sci.chem.analytical,sci.bio.misc,sci.techniques.microscopy,sci.med.laboratory Subject: FOR SALE: Inverted Microscope Date: 18 Jan 1996 16:07:04 GMT Organization: University of Pennsylvania Lines: 20 Message-ID: <4dlr78$i4u@netnews.upenn.edu> NNTP-Posting-Host: pobox.upenn.edu X-Newsreader: TIN [version 1.2 PL2-upenn1.3] Xref: biosci bionet.neuroscience:12126 bionet.biophysics:1601 sci.chem:47420 sci.chem.labware:1644 sci.chem.analytical:2383 sci.bio.misc:1686 sci.techniques.microscopy:3776 sci.med.laboratory:336 ***Zeiss Axiovert: in absolutely mint condition 10, 20 40 x (dry) and 63 x oil immersion objectives .55 N.A. long working distance condenser Bright field, phase and DIC Motorized nose piece Large mechanical stage with long control arm 1.6 or 2.5 x optivar Binocular phototube, with focussing C-mount for TV camera Current new price is $42,746. Selling for half of this ($21,373). -- Geoffrey H. Gold gold@pobox.upenn.edu Monell Chemical Senses Center 3500 Market St. Philadelphia, PA 19104-3308 Phone: 215-898-5199 Fax: 215-898-2084 From owner-biophysics@net.bio.net Wed Jan 17 22:00:00 1996 Path: biosci!ICT.SLD.CU!NORMANDO From: NORMANDO@ICT.SLD.CU (Normando Iznaga) Newsgroups: bionet.biophysics Subject: (none) Date: 17 Jan 1996 21:56:46 -0800 Organization: Centro de Inmunologia Molecular Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <9601172013.AA25946@infomed> NNTP-Posting-Host: net.bio.net unsubscribe end !*************************************! ! M. Sc. Normando Iznaga Escobar ! ! Center of Molecular Immunology ! ! Division of Antibody Engineering ! ! PO BOX 16040, Havana, 11 600, Cuba ! ! ! ! Fax Number : (53 7) 33 50 49 ! ! (53 7) 33 35 09 ! ! Phone : (53 7) 21 68 11 ! ! (53 7) 21 79 33 ! ! e-mail : normando@ict.sld.cu ! !*************************************! From owner-biophysics@net.bio.net Wed Jan 17 22:00:00 1996 Path: biosci!JHUNIX.HCF.JHU.EDU!wong_cy From: wong_cy@JHUNIX.HCF.JHU.EDU (Cingyuen Wong) Newsgroups: bionet.biophysics Subject: housing for biophysical society meeting Date: 17 Jan 1996 16:44:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <96Jan17.194209edt.2849-7@jhunix.hcf.jhu.edu> NNTP-Posting-Host: net.bio.net This is being posted for a friend. Female graduate student needs to find housing for 1996 Biophysical Society meeting in Baltimore. If you have a hotel room and need a roommate, please email her directly at rionescu@sunset.backbone.olemiss.edu Thank you From owner-biophysics@net.bio.net Wed Jan 17 22:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!news.forth.gr!news.auth.gr!usenet From: Zanos Stavros Newsgroups: bionet.biophysics Subject: Re: Spikes analysis Date: 17 Jan 1996 01:59:31 GMT Organization: Aristotle Univ of Thessaloniki School of Medicine Lines: 12 Message-ID: <4dhl63$mut@evia.ccf.auth.gr> References: <4d6be8$d3a@tabarca.cpd.ua.es> NNTP-Posting-Host: antigoni.med.auth.gr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) I'm not currently aware of any program of that kind. However, you could find it useful to look at the following paper: C. N. Christakos (1994) Analysis of Synchrony (Correlations) in Neural Populations by Means of Unit-to-Aggregate Coherence Computations. Neuroscience, 58(1): 43-57 Zanos Stavros Aristotle Univ School of Medicine Thessaloniki, Macedonia, Greece From owner-biophysics@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!gatech!usenet.eel.ufl.edu!psgrain!fizban.solace.mh.se!demos!dnews-server From: "Zubrianov Igor L." Newsgroups: bionet.biophysics Subject: Research project for grant-HELP to find Date: 19 Jan 1996 06:43:31 +0300 Organization: Firm AMITY Lines: 163 Sender: news-server@news.demos.su Distribution: world Message-ID: Reply-To: igor@amity.alma-ata.su NNTP-Posting-Host: root@news.demos.su X-mailer: dMail [Demos Mail for DOS v1.23] X-Return-Path: news.demos.su!kremvax.demos.su!ricc.alma-ata.su!amity!amity.alma-ata.su!igor 480072, Republic of Kazakstan, Almaty, pr.Abai, 91 tel. 7-3272-679062 E-mail: igor@amity.alma-ata.su Deformability and aggregation of human erythrocytes in the presece of ethanol. Director of AB IPB, Candidate of Biology, Balmukhanov Boris Saimovich US 25 200 dollars, have not submitted, have not applied 1.registered 2. 28 persons 3. no grants was received I. d) national budget, The Ministry of Science and new technology of the Republic of Kazakstan e) Since AB IPB has been organized (August 1994) the personnel has been taking part in the Republic scientific and technological Program "The development of the application of biological technology to agroindustrial complex and public health". II. A). In spite of the fact that the changes of structural-functional states of erythrocytes under chronic alcoholism (pernicious anaemia) are well-known, the influence of ethanol on normal human erythrocytes in doses corresponding to the minimum level of intoxication was less investigated and it is unknown if a normal dose of ethanol in the blood can influence on its rheological characteri- stics. For the last decade the results of the investigations of the red blood cell (RBC) deformability have been widely used in experimental and clinical haemorhe- ology. The index of deformability is calculated from the RBC capacity to pass through capillaries and pores with diameters, which are smaller than their own sizes. Distortions of RBC deformability are observed not only in case of ancest- ral defects of a structure, but they also appear in response to the chronic deseases. By this time it has been shown that deformability depended on the ratio of a square to a volume, viscousity of the intracellular content, changes of the proportions of phospholipid, included into internal and external layers of bilayer membrane (asymmetry of a phospholipid component of RBC surface), their phophorylation, variation of ATP and Ca, under reversible phase transitions in membrane, caused by the changes of incubation temperature in vivo. The changes of deformability are observed in response to the influence of various medicina l remedies in vivo and in vitro. Being a parameter indicating multifunctional changes in a cell, deformability is very sensitive for the presence of membranotropic substances of various chemical structures. Besides the changes of deformability can be noticed under smaller doses of the investigated agent than those ones, under which the changes of RBC shapes ( crenation, cup and sphered formation) are manifested. In this connection there is a problem of a possible influence of ethanol (amount of which depends on the individual activity of enzyme system being concerned in metabolism of ethanol and on the character of food, and normally presented and synthesized by microflora in the blood ) on RBC deformability. The results of this study can be useful not only for normal physiology, but also for the understanding of the degree of microrheological distortions under high level of intoxication and at the different stages of chronic alcogolism. B) This problem concerns specialists of cell suspensions rheology and pharmacists studing mechanisms and effects of various haemorheocorrectors. The results of the project can be also useful for the specialists studing physical and chemical mechanisms of RBC aggregation and the influence of aggregation on nonlinear- viscous properties of the blood. In former Soviet Union similar investigations are going at the haemotological centers (e.g. Russia Haemotological Center, laboratory of physical biological chemistry ), departments of medical institutes (Central Research Laboratory of Kazak Medical Institute) and universities (biological physics department of physics faculty of Moscow State University). C) AB IPB was organized for biological tests to select substances with expected pharmaceutical properties. Any determination of new mechanisms of RBC aggregation willbe used to develop test-systems for the selection of substances to correct haemorheological disturbances. III. Determination of minimum concentration of ethanol reducing RBC capacity for deformation and reversible aggregation, and valuation of a possible influence of endogeneous ethanol, normally presented in the blood, on a microcurculation. IV. 1. Study of the influence of ethanol and acetaldehyde of various concentrations on deformability and reversible aggregation of human and experimental animals RBC in vitro. 2. Study of deformability and reversible aggregation of experimental animals RBC in response to the changes of ethanol concentration in the blood, caused by the changes of a food content. 3. Study of deformability and reversible aggregation of experimental animals RBC after various doses of ethanol. 4. Study of deformability of human RBC of volunteers' blood after various doses of ethanol. V. A) 1. Selection of human and experimental animals RBC isolation and incubation conditions providing RBC volume and shape constancy, and minimum divergence of control data of deformability. 2. Comparison of quantitative characteristics of RBC aggregation, obtained by the sedimentational and optical methods in case of a control and in the presence of ethanol. 3. Measurements of deformability of human RBC, suspended in saline solutions, in the presence of ethanol and acetaldehyde of various concentrations with filtration method in vitro. VI. Expences 1. Chief 350 1 12 4200 research worker 250 3 12 9000 laboratory assistant 150 1 12 1800 total 15000 3. - 360 2 720 - 30 2 10 days 600 - 20 2 10 days 400 total 1720 4. - 200 1 12 2400 - 50 12 600 - 20 12 240 - 20 12 240 total 3480 5. balance 1500 filters 500 aggregometer 3000 total 5000 total of the project 25 200 VII A) The results of the project can be evaluated by the comparison of ethanol concentrations, under which the changes of RBC deformability and reversible aggregation are observed in vitro, with the concentrations of ethanol in human and experimental animals blood, under which the changes of microcurculation are observed by the methods of blood circulation physiology in vivo. B) rotational viscometer, provided with the optical chanel for the parallel measurements of aggregation. C)Recomendations 1.Moscow State University, the Chairman of biological physics department Tverdislov Vsevolod Aleksandrovich 2.Russia Haemotological Center, the Chairman of physical biological chemistry laboratory Ataullakhanov Fasli VIII. 1. The results of the project would be useful for AD IPB, since it stimulates an organozation of complex measurements of deformability, reversible aggregation, RBC sizes distribution, and also a collection of information about the influence of ethanol and other aliphatic spirits on a structure and chemical components of RBC membrane in case of the consideration of their viscous-elastic properties. The influence of ethanol on a function of various parts of organism has been widely investigated, but in a connection of the individuality of human organism, RBC has not been studied yet enough. So the results of the project could be useful for the practical needs of medicine as well. 2. The study of the influence of ethanol on RBC is an important stage for the complex investigations of the influence of medicinal remedies on microcurcula- tion, since the interaction of aliphatic spirits with the phospholipid membrane is closely studied in various fields of science. The scientific basis of the project could take into account a new factor influencing on microcurculation inside of a normal organism and in case of pathology, like various levels of intoxication and chronic alcoholism. 3. I am interested in the study of the disturbancies of normal phospholipid distribuitions in RBC membrane under the influence of ethanol and affined combinations. In this connection RBC can imitate the behaviour of white blood cells in response to the inflammatory processes and accompanying immune reactions. 4. The results of the study of microcurculation of human and experimental animals RBC in the presence of ethanol and acetaldehyde of various concentrations will be used for the further search for the medicinal agents accelerating the rehabilitation of RBC properties. Chief of project, Candidat of Biology, B.Balmukhanov From owner-biophysics@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!swrinde!sdd.hp.com!col.hp.com!csn!magnus.acs.ohio-state.edu!slip1-25.acs.ohio-state.edu!ealcanta From: ealcanta@postbox.acs.ohio-state.edu (edwin alcantara) Newsgroups: bionet.biophysics Subject: lipid bilayer Date: Fri, 19 Jan 1996 02:24:12 GMT Organization: The Ohio State University Lines: 4 Message-ID: NNTP-Posting-Host: slip1-25.acs.ohio-state.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #1] What is the best way to monitor the formation of planar lipid bilayer? Is it also a good idea to fuse a protein into liposome prep before incorporation into the bilayer. If so, is there a protocol for the preparation of liposomes that I can refer to? Thanks in advance. From owner-biophysics@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!vixen.cso.uiuc.edu!howland.reston.ans.net!torn!nott!nrcnet0.nrc.ca!BRI.NRC.CA!Feng.Ni From: Feng.Ni@BRI.NRC.CA (Feng Ni) Newsgroups: bionet.biophysics Subject: RESEARCH ASSOCIATE POSITIONS IN PROTEIN NMR Date: 19 Jan 1996 00:12:00 GMT Organization: Institut de recherche en biotechnologie, Montréal Lines: 46 Distribution: world Message-ID: <4dmnkg$fg9@nrcnet0.nrc.ca> NNTP-Posting-Host: indy.bri.nrc.ca RESEARCH ASSOCIATE POSITIONS IN PROTEIN NMR SPECTROSCOPY AND MOLECULAR DESIGN Candidates are being sought to fill a research associate position in the NMR laboratory at the Biotechnology Research Institute (Montreal, Quebec, Canada). This is a two-year limited term appointment from the date of reporting with a salary of CND $38-45K/annum. The successful candidate will determine the bioactive conformations of peptides and protein fragments, study protein-peptide interactions by use of multi- dimensional NMR spectroscopy and participate in the design of novel molecules through the use of the NMR structures of peptides and proteins. This work is part of a multidisciplinary effort for the design of inhibitors of protease regulation and signal transduction. Specific protein targets include blood coagulation factors (e.g., thrombin), cysteine protease cathepsins, growth factor receptors and protein tyrosine phosphotases (PTPases). The proteins or their fragments for NMR analysis include EGF-like domains, kringle proteins, protease activation fragments and SH2 domains. You are expected to lead your own projects and to collaborate with other members of the Institute and with industrial scientists. Applicants must demonstrate within the content of their application that they meet the following screening criteria in order to be given further considera- tion as candidates: 1) a recent Ph.D. in chemistry, biochemistry or a related field, and solid training in protein structure and protein biochemistry, 2) research experience in conformational study of peptides and structure deter- mination of proteins by use of multidimensional NMR spectroscopy. The candidates will be assessed on the basis of the following criteria: 1) knowledge of the principles of protein structure, the solution behavior of peptides and proteins and the advantages and limitations of NMR spectroscopy for conformational analysis and structure determination, 2) familarity with the acquisition and processing of homo- and hetero-nuclear NMR data using state-of-the-art NMR hardware and software, 3) demonstrated abilities to plan and lead research projects and to carry out interdisciplinary collaborations with scientists from different fields, 4) abilities and skills to write peer- reviewed scientific articles (as demonstrated by an excellent publication record) and to communicate clearly and effectively in oral presentations, and 5) demonstrated ability to work independently as well as a member of a multidisciplinary team. Applications, which demonstrate qualifications for the above requirements, may be submitted to the e_mail address: fengni@bri.nrc.ca or by regular mail to: Dr. Feng Ni, Biotechnology Research Institute, 6100 Royalmount Avenue, Montreal, Quebec, H4P 2R2 Canada or by fax to: (514)-496-6232 c/o Dr. Feng Ni. From owner-biophysics@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!BBRI.HARVARD.EDU!STAFFORD From: STAFFORD@BBRI.HARVARD.EDU (Walter Stafford) Newsgroups: bionet.biophysics Subject: National Analytical Ultracentrifugation Workshop Date: 19 Jan 1996 14:15:43 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 254 Sender: daemon@net.bio.net Distribution: world Message-ID: <960119171258.28ee@bbri.harvard.edu> NNTP-Posting-Host: net.bio.net Subj: National AUC Workshop Announcement ------------------------------------------------------------------------ A web version of the National AUC Workshop Announcement can be obtained from http://wfs.bbri.harvard.edu/xlameet.htm ---------------------------------------------------------------------- ANALYTICAL ULTRACENTRIFUGATION Theory and Practice WORKSHOP March 19-21, 1996 and Symposium March 22, 1996 National Analytical Ultracentrifugation Facility Biotechnology Center, University of Connecticut, Storrs, Connecticut, USA * Interact with national experts in analytical ultracentrifugation. * Learn characterization of recombinant produced proteins * Work with hardware and software developed on-site at the National Analytical Ultracentrifugation Facility. * Receive copies of all data analysis for your subsequent use. * Receive individual attention to your research interests. * See the Beckman XL-A Analytical Centrifuges in Operation * See real time interferometry in operation on Model E and Beckman XL-I analytical ultracentrifuges. * Learn about new specialized centrifuge cells. * Receive registration for Analytical Ultracentrifugation symposium to follow workshop. INTENT and DESCRIPTION Analytical Ultracentrifugation (AU) is a powerful method for the determination of absolute molecular weights and the study of self-association and interactions between biological molecules in solution. The combination of new computerized instrumentation, more rapid experimental protocols, and powerful data analysis techniques have led to a resurgence of interest in AU, particularly in biotechnology and pharmaceutical research. The AU Facility in the Biotechnology Center was established in 1988 by grants from the National Science Foundation and the Connecticut Department of Higher Education. Creation of the facility follows the fundamental contributions of its staff to the development of AU to a wide range of basic and applied problems for over a quarter century. The staffed facility houses both Beckman Model E and XL-A analytical ultracentrifuges with their associated equipment. The facility is available to investigators for basic research through facility scientists or by collaboration with one of its principal investigators. Research projects from industry are encouraged. The Analytical Ultracentrifugation Facility is pleased to offer an AU workshop designed for professionals with some previous knowledge of AU including: * Industrial Scientists * Academic Scientists * Research Technicians * Students The workshop will provide a conceptual framework and hands-on experience in modern computer-assisted data acquisition and data analysis for molecular weight determination, for study of molecular association-dissociation reactions and for determination of attendant equilibrium constants, with applications to proteins, glycoproteins and nucleic acids. One session will be devoted to development of experimental strategies for designing various kinds of molecular characterization problems. Sponsored by the UConn Biotechnology Center, Beckman Instruments, Inc., UConn Department of Molecular & Cell Biology, Digital Equipment Corporation, Genentech, Inc., National Science Foundation, Perkin Elmer, Pfizer, Inc., SmithKline Beecham Pharmaceuticals, and others to be announced at the workshop. SPECIAL PROGRAM FEATURES: * Limited workshop size for maximum interaction * Interactive lecture sessions * Data analysis software furnished to all participants The registration fee of $1500 (or $800 for registrants of not-for-profit organizations) includes all costs of instruction, materials, software, parking, refreshments, lunches and reception. The fee does not include hotel accommodations. Application is necessary. Faxed (203-486-5005) applications are encouraged. A letter of acceptance will include information on housing, transportation and an invoice for the registration fee, as appropriate. For Information on: * Course content: call Todd Schuster at 203-486 4333 * Payments and course logistics: call the Biotechnology Center at 203-486-5011 * Fellowships and Applications: call 203-486-5011 * E-mail: Biotctrl@uconnvm.uconn.edu Some registration fellowships are available to qualified graduate and post-doctoral students. Call or send for a fellowship application form. Refunds and Cancellations The registration fee is fully refundable prior to the first day of the program. Registrants who do not attend and do not cancel are subject to the complete fee. Participant substitutions may be made with prior approval of the teaching staff. ---------------------------------------------------------------------------- Application Form: Fax to (203) 486-5005 Analytical Ultracentrifugation: Theory and Practice March 19-21, 1996 Symposium March 22, 1996 National Analytical Ultracentrifugation Facility Biotechnology Center, University of Connecticut, Storrs, Connecticut 06269-3149 Name: _____________________________________ Name to put on badge: _______________________ Social Security #: ___________________________ Phone (day): _______________________________ Organization: _______________________________ Organization Address:_________________________ Street: ____________________________________ City: ______________________________________ Phone (evening): ____________________________ Fax:_______________________________________ Registration fee: check one ____$1500 Workshop and Symposium ____$800 (not-for-profit organization) ____$200 March 22 Symposium only Method of Payment (once accepted) ____Bill me ____Purchase Order enclosed. Number: ___________________________________ end application form ---------------------------------------------------------------------------- PROGRAM: Review of basic theory and practice * sedimentation velocity * sedimentation equilibrium Demonstration of basic experimental procedures using * Beckman Model E Analytical Centrifuge * Beckman XLA Analytical Centrifuge * optical absorption scanner method * Rayleigh interferometry * short and long column equilibrium sedimentation * velocity sedimentation Demonstration of equilibrium techniques Illustrations of experimental systems using proteins, glycoproteins, and nucleic acids * simple proteins exhibiting ideal behavior * non-ideal behavior * interacting systems * heterogeneous systems Data collection demonstrating * real-time interferometry * absorption scanning Data analysis and interpretation portable software furnished to participants for analytical procedures LEARNING OBJECTIVES Participants will: * be introduced to basic theory and practice of sedimentation velocity and equilibrium; * be shown how to design experiments to characterize macromolecular interactions; * use computer software for data analysis; * participate in small group discussions. INSTRUCTORS: Emory Braswell, Professor, Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut. Michael Johnson, Professor of Pharmacology and Internal Medicine, University of Virginia Health Science Center, Charlottesville, Virginia. Jeffrey Lary, Research Associate, Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut. Thomas Laue, Associate Professor, Department of Biochemistry, University of New Hampshire, Durham, New Hampshire. Todd Schuster, Professor, Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut. Steven Shire, Senior Scientist, Pharmaceutical Research and Development Department, Genentech, Inc., South San Francisco, California. Walter Stafford, Senior Scientist, Analytical Ultracentrifugation Research Laboratory, Boston Biomedical Research Institute, Boston, Massachusetts. John M. Toedt, Graduate Research Assistant, Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut. Jia-wen Wu, Facility Engineer, Analytical Ultracentrifugation Facility, University of Connecticut, Storrs, Connecticut. David Yphantis, Professor, Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut. Dan Zhu, Facility Scientist, Analytical Ultracentrifugation Facility, University of Connecticut, Storrs, Connecticut. Symposium following the workshop, March 22, Call for Symposium Speaker List after 1/15/1996 From owner-biophysics@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!uwm.edu!lll-winken.llnl.gov!usenet From: Chris Barry Newsgroups: bionet.biophysics Subject: Re: Fluorescent labeling of DNA Date: Fri, 19 Jan 1996 11:46:22 -0800 Organization: Lawrence Livermore Lines: 8 Message-ID: <30FFF50E.62AFB06F@mackiller.llnl.gov> References: <4do79l$4hc@mserv1.dl.ac.uk> NNTP-Posting-Host: mackiller.llnl.gov Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b4 (X11; I; Linux 1.1.72 i486) Direct incorporation of fluorochromes/label by PCR of probes Labeling of probes by end labeling Boehringer Mannheim is a good source Chris From owner-biophysics@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!swrinde!howland.reston.ans.net!EU.net!peer-news.britain.eu.net!sunsite.doc.ic.ac.uk!daresbury!not-for-mail From: f.munkonge@ic.ac.uk (Dr F Munkonge) Newsgroups: bionet.biophysics Subject: Fluorescent labeling of DNA Date: 19 Jan 1996 13:45:25 -0000 Lines: 8 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4do79l$4hc@mserv1.dl.ac.uk> X-Sender: fmhl@tolstoy.nhli.ic.ac.uk Original-To: biophys@dl.ac.uk Could anyone please enlighten me on the state of the art in labeling DNA with fluorescence molecules (especially FITC and TRITC). Leads on commercial sources would also be gratefully appreciated. Thanks Felix (f.munkonge.icl.ac.uk) From owner-biophysics@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!newsfeed.internetmci.com!in2.uu.net!demos!dnews-server From: "Zubrianov Igor L." Newsgroups: bionet.biophysics Subject: NEED GRANT:Influence of non-equilibrium states of a neer-membrane electrolyte layer close to cell surface on reversible RBC aggregation ...(etc) Date: 19 Jan 1996 13:57:00 +0300 Organization: Firm AMITY Lines: 8 Sender: news-server@news.demos.su Distribution: world Message-ID: Reply-To: igor@amity.alma-ata.su NNTP-Posting-Host: root@news.demos.su X-mailer: dMail [Demos Mail for DOS v1.23] X-Return-Path: news.demos.su!kremvax.demos.su!ricc.alma-ata.su!amity!amity.alma-ata.su!igor Please, advice us where we can receive grant for this project: The study of the influence of non-equlibrium states of a neer-membrane electrolyte layer close to cell surface on reversible erythrocytes aggregation and formation of non-Newtonian hydrodynamic properties of blood. Republic of Kazakhstan, Almaty, Institute of Pharmaceutical Biotechnology. E-mail: igor@amity.alma-ata.su From owner-biophysics@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!in2.uu.net!demos!dnews-server From: "Zubrianov Igor L." Newsgroups: bionet.biophysics Subject: NEED GRANT: Deformability and aggregation of human erythrocytes in the presence of ethanol. Date: 19 Jan 1996 13:48:27 +0300 Organization: Firm AMITY Lines: 6 Sender: news-server@news.demos.su Distribution: world Message-ID: Reply-To: igor@amity.alma-ata.su NNTP-Posting-Host: root@news.demos.su X-mailer: dMail [Demos Mail for DOS v1.23] X-Return-Path: news.demos.su!kremvax.demos.su!ricc.alma-ata.su!amity!amity.alma-ata.su!igor Please, advice us how to receive grant for the this research project. Republic of Kazakhstan, Almaty, Institute of Pharmaceutical Biotechnology. Thank's in advance. E-mail: igor@amity.alma-ata.su From owner-biophysics@net.bio.net Fri Jan 19 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!info.ucla.edu!library.ucla.edu!ucsbuxb.ucsb.edu!nntp.ucsb.edu!usenet From: John Perona Newsgroups: bionet.biophysics Subject: POSTDOCTORAL POSITION - STRUCTURAL ENZYMOLOGY Date: 19 Jan 1996 23:45:30 GMT Organization: University of California, Santa Barbara Lines: 40 Message-ID: <4dpaeq$b7e@yuggoth.ucsb.edu> NNTP-Posting-Host: sake.chem.ucsb.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.12 (X11; I; IRIX 5.2 IP22) X-URL: news:bionet.biophysics POSTDOCTORAL POSITION STRUCTURAL ENZYMOLOGY An NIH-funded postdoctoral position is available to use a combination of enzyme kinetic and crystallographic approaches to explore specific DNA cleavage by the EcoRV endonuclease. Our approach is to correlate pre-steady state kinetic measurements with crystal structures, for a variety of mutant enzymes exhibiting altered DNA binding and catalytic functions. A parallel project is also underway which explores the use of genetic selection methods in E. coli for the isolation of EcoRV mutants with altered substrate specificity profiles. This project is intensively structure-based and provides a bridge for the classical enzymologist or protein crystallographer to acquire new training at an interface where both perspectives are required. Very few enzymes have been simultaneously studied by the combination of pre-steady state kinetics and crystallographic analyses of mutants. We believe that this integrated approach has some substantial potential to provide a database of information crucial to rational re-engineering of existing protein scaffolds to new function. Some of the specific questions we will be exploring in the immediate future are: (1) the stru