From owner-biophysics@net.bio.net Sun Aug 04 23:00:00 1996 Path: biosci!LEX.LCCC.EDU!rcb1 From: rcb1@LEX.LCCC.EDU (Ron Blue) Newsgroups: bionet.biophysics Subject: Re: Reversal potential Date: 5 Aug 1996 04:55:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 62 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <199608011844.OAA12265@boris.interlynx.net> NNTP-Posting-Host: net.bio.net On 1 Aug 1996, Lars Thomsen wrote: > Sorry about my joke - I couldn't help it. But the effect you describe > is that really correlated to the reversal potential and how ? > Lars Thomsen, MS PhD Ask me to send you the following document by email. Ron Blue Abstract: The correlational opponent-processing theory is a neuro homeostasis integration psychological immune theory that would connect phenomena such as sensation, perception, movement, habituation, memory, representations, learning, cognition, personality, psychopathology, paradoxical integration, emotion, and evolution of the mind under a unified theory. Perception/learning/cognition may be viewed as an effort to assimilate and accommodate all experience into neuro-energy-efficient eigenfunction equivalence or quasi-holographic correlational opponent- processing recordings. Stimuli causes brain wave modulations which interact with carrier or reference wavelets. This interaction creates a quasi-holographic stimulus wavelet. The opponent-process creates an opposing quasi- holographic memory wavelet. Through this process the correlations or associations of experience are encoded to memory. Every wavelet, regardless of source or type, triggers an opposing wavelet. The function of the opposing wavelet or feedback is to diminish the intensity of neural processing. A wavelet potential is stored or hard wired as long-term potentiation opponent-processes in nerve cells and the interconnections between nerve cells. The wavelets are quasi- holographic and allow recovery of information due to the interaction of reference carrier wavelets and stimuli, thought, motor movement, and emotional arousal. Outline: Discussion Neuro Net Quasi-holographic wavelets Habituation/immunization Memory Representations, copies or models Learning/Cognition Personality Sensations and Perceptions Movement Emotion Evolution Tools Implications Conclusion and applications from COP theory Discorrelation Education Biophysical Intelligence Defense Mechanisms Brain damage Creativity Brain Tape Computer Model Conclusion Bibliography Acknowledgments From owner-biophysics@net.bio.net Sun Aug 04 23:00:00 1996 Path: biosci!NANOTHINC.COM!tess From: tess@NANOTHINC.COM (Tess Mercer) Newsgroups: bionet.biophysics Subject: Nanobiology Call for Papers Date: 5 Aug 1996 14:21:41 -0700 Organization: Nanothinc Lines: 57 Sender: daemon@net.bio.net Distribution: world Message-ID: <32060438.1A6B@nanothinc.com> Reply-To: tess@nanothinc.com NNTP-Posting-Host: net.bio.net Topical Overview: Call for Papers - Nanobiology: Research, Theory, and Current Applications Nanotechnology, as a general topic, covers a very wide array of different disciplines and areas of research. However, in the realm of biological and biomolecular applications and developments, there are a number of current examples, including some commercial applications, which are reshaping the approach to which previously difficult, or even "unsolvable" procedures can now be resolved, or greatly improved upon. It is specifically in the realm of thoeretical, near term, or currently available technologies, and how they are affected by application of "nanobiological" processes, that will be reviewed in this section of the journal. By definition, a nanobiological process could be described, in this context, as any process which utilizes the contrived manipulation of individual biomolecules or biomolecular structures to fullfill a desired task, create an object, a material or application of a material, or as an intermediate step in fullfilling a multistaged series of assembly or construction tasks involving biomolecular materials. This definition could also be expanded to include the methodologies of visualiztion or detection of nanoscale activities in a biomolecular environment, or in the development of instrumentation and processes related to these tasks. Also, there are other tangential aspects of nanobiological processes which are also relevant, and deserve attention. Particularly in the areas of potential nanoscale computing or information processing applications which may utilize or incorporate biological components or processes, there has been considerable interest and research ativities. Please let us know if you are interested. Information about our company, Nanothinc, can be found at http://www.nanothinc.com. Also, I have additional Notes for Contributors which I can fax to your attention. Sincerely, Maritess T. Mercer Director of Administration Project Manager Nanothinc 1797 Union Street San Francisco, CA 94123 Tel: (415) 202-9969, Fax: (415) 202-9975 URL: http://www.nanothinc.com Email: tess@nanothinc.com From owner-biophysics@net.bio.net Sun Aug 04 23:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!newsxfer2.itd.umich.edu!portc01.blue.aol.com!newsstand.cit.cornell.edu!news.acsu.buffalo.edu!dsinc!news.enter.net!usenet From: rcarver@enter.net (Rowland Carver) Newsgroups: bionet.biophysics Subject: Re: loose patch clamp Date: 5 Aug 1996 21:17:05 GMT Organization: Lehigh Valley Computer Group Lines: 38 Message-ID: <4u5och$b1i@news.enter.net> References: NNTP-Posting-Host: pm2-15.enter.net Mime-Version: 1.0 X-Newsreader: WinVN 0.99.4 Yes my friend, if you are serious, open it up and solder a jumper across the burned section...DR. Done In article , wmfry@morgan.ucs.mun.ca says... > > >Hi. > >I have an LPC-3 loose patch clamp amplifier from medical systems corp >(/List-electronics) with a burned out potentiometer. The bum pot is the >motorized one that controls leakage compensation, and I can't find >another anywhere. It is a 10k, 10 turn pot, but it has no part number. >I think it may have been manufactured by Prazisions Elektronik / H. >Knuppel of West Berlin. > >Medical Systems Corp of Greenvale NY can't help me at all, I guess the >amp is too old. > >Is there anybody out there who knows where i can find a suitable >replacement part. Maybe somebody has cannibalized an old LPC-3 for parts >and has not used this one. > >Or even still, maybe somebody knows some neat tricks i can use to modifey >this one. > >thanks for any help. > >Mark Fry ribit @..@ >4th floor BMS Fac of Med. \ (----) >Memorial University of NFLD ( >**< ) >A1B 3V6 ^^^""""^^^ >wmfry@plato.ucs.mun.ca (Xenopus) > From owner-biophysics@net.bio.net Mon Aug 05 23:00:00 1996 Path: biosci!agate!newsgate.duke.edu!news-feed-1.peachnet.edu!usenet.eel.ufl.edu!news.bright.net!chi-news.cic.net!news.luc.edu!orion!csriniv From: csriniv@orion.it.luc.edu (Chandra Srinivasan) Newsgroups: bionet.biophysics Subject: Postdoctoral Position Date: 6 Aug 1996 04:55:00 GMT Organization: Loyola University Chicago Lines: 10 Message-ID: <4u6j74$9n2@artemis.it.luc.edu> NNTP-Posting-Host: 147.126.1.9 NNTP-Posting-User: csriniv X-Newsreader: TIN [version 1.2 PL2] August 5, 1996 POSTDOCTORAL POSITION available immediately to investigate the pharmacological action of lithium in the treatment of manic-depression in NIMH-funded project. One year appointment that can be extended for a second year subject to performance. Experience in NMR spectroscopy and biochemical methods preferred. Send c.v. and three letters of recommendation to Professor Duarte Freitas, Department of Chemistry, Loyola University, 6525 North Sheridan Road, Chicago, IL 60626. From owner-biophysics@net.bio.net Mon Aug 05 23:00:00 1996 Path: biosci!agate!newsgate.duke.edu!news-feed-1.peachnet.edu!usenet.eel.ufl.edu!news.ultranet.com!homer.alpha.net!uwm.edu!spool.mu.edu!torn!news.ccs.queensu.ca!kone!nott!standby.nrc.ca!BRI.NRC.CA!Feng.Ni From: Feng.Ni@BRI.NRC.CA (Feng Ni) Newsgroups: bionet.biophysics Subject: RESEARCHER POSITIONS IN PROTEIN NMR SPECTROSCOPY Date: 5 Aug 1996 22:21:49 GMT Organization: Institut de recherche en biotechnologie, Montréal Lines: 44 Distribution: world Message-ID: <4u5s5t$6a1@standby.nrc.ca> NNTP-Posting-Host: bravais.bri.nrc.ca RESEARCHER POSITIONS IN PROTEIN NMR SPECTROSCOPY The NMR laboratory of the Biotechnology Research Institute (BRI) have openings for self-motivated young researchers in the area of biomolecular/protein NMR spectroscopy. Candidates should have a recent Ph.D. in chemistry, biochemistry or a related field and have demonstrated abilities in carrying out creative research. Experiences include the determination of protein structures and/or the study of protein-ligand interactions by use of triple resonance and multi- dimensional NMR spectroscopy. Knowledge in isotope labelling and protein/pep- tide purification is an important asset. Successful candidates will normally start in the postdoctoral level, or depending on qualifications, may be recruited directly to more senior positions offerred by the National Research Council of Canada. The Research Council offers competitive salaries and other compensation packages commensurate upon your experience and appointment levels. The NMR laboratory at BRI is equipped with one Bruker AMX2-500 MHz spectrometer and shares a Bruker DRX-500 MHz spectrometer with members of the Montreal Joint Centre for Structural Biology. A new state-of-the-art 750 MHz NMR instrument will be added to the facility within the coming year. NMR researchers also have access to excellent computing facilities and work in multidisciplinary teams with expertise in molecular biology, protein/peptide chemistry, molecular design and structural biology. The Biotechnology Research Institute (BRI) is a branch of the National Research Council of Canada, interfacing academia and industry. Your challenge at BRI will be to succeed in high-impact scientific research while generating prac- tical applications transferable to pharmaceutical industry. Qualified indivi- duals are invited to submit your resume, along with the names and addresses of three references, to: Feng Ni, Ph.D. Head of Biomolecular NMR Biotechnology Research Institute 6100 Royalmount Avenue Montreal, Quebec, H4P 2R2, Canada phone: (514)-496-6729; fax: (514)-496-5143 e_mail: fengni@bri.nrc.ca Applications will be accepted until the positions are filled. We thank all applicants for their interests, however, only those being considered will be contacted. From owner-biophysics@net.bio.net Mon Aug 05 23:00:00 1996 Path: biosci!PHYSLOG-PO.PHYSLOG.UIOWA.EDU!Hoshi From: Hoshi@PHYSLOG-PO.PHYSLOG.UIOWA.EDU Newsgroups: bionet.biophysics Subject: Postdoc opportunities in ion channel research Date: 6 Aug 1996 07:35:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: <199608061435.JAA14207@ns-mx.uiowa.edu> NNTP-Posting-Host: net.bio.net POSTDOCTORAL OPPORTUNITIES IN ION CHANNEL RESEARCH Postdoctoral opportunities are available in my laboratory to study how ion channels gate and how their properties affect the organism's higher functions. In particular, we focus on potassium channels modulated by intracellular factors, such as pH, oxidation/reduction, and cGMP/cAMP. Electrophysiological skills (two-electrode voltage clamp, patch-clamp, cut-open oocyte clamp), single-channel analysis skills, protein biochemistry/molecular mutagenesis skills, and neurophysiology knowledge are useful in the laboratory. Toshi Hoshi, Ph.D. Department of Physiology and Biophysics Bowen 5660 The University of Iowa Iowa City, IA 52242 Toshinori-Hoshi@uiowa.edu FAX: 319-335-7330 From owner-biophysics@net.bio.net Tue Aug 06 23:00:00 1996 Path: biosci!biosci!not-for-mail From: a.c.noorlandt@chem.ruu.nl (Albert Noorlandt) Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.immunology,bionet.metabolic-reg,bionet.molbio.ageing,bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.molbio.proteins.7tms_r,alt.med,alt.pharm,sci.bio.misc,sci.chem,sci.chem.analytical,sci.med,sci.misc,sci.techniques Subject: 1997 FEBS Meeting on Cell Signalling Mechanisms Date: 6 Aug 1996 17:24:42 -0700 Organization: CBLE Lines: 29 Sender: biohelp@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Xref: biosci bionet.biophysics:2198 bionet.cellbiol:5225 bionet.immunology:9491 bionet.metabolic-reg:805 bionet.molbio.ageing:2916 bionet.molbio.methds-reagnts:47700 bionet.molbio.proteins:8451 bionet.molbio.proteins.7tms_r:804 sci.bio.misc:4117 sci.chem:61653 sci.chem.analytical:5118 sci.med:133749 sci.misc:12601 FEBS Special Meeting on Cell Signalling Mechanisms, From membrane to nucleus Amsterdam, The Netherlands, June 29­July 3, 1997 Everything you want to know about this meeting on www-page: http://www.cble.chem.ruu.nl/febs97/index.html The meeting will cover the following topics: € Tyrosine kinases € MAP-, Ser/Thr-kinases € G-coupled receptors € Lymphokine receptors € Ras, Rac, Rho-proteins € Ca2+, cGMP, NO € Lipid signalling € Steroid receptors € Transcription factors € Cell cycle, Apoptosis For further information contact: Secretariat FEBS Special Meeting 1997 Lidy Groot Congress Events P.O. Box 83005 1080 AA Amsterdam The Netherlands Tel. +31.20.679 3218 Fax: +31.20.675 8236 Email: lidy.groot@inter.nl.net From owner-biophysics@net.bio.net Tue Aug 06 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!news.ac.net!news.bconnex.net!news2.insinc.net!roger.interlynx.net!news From: lthomsen@interlynx.net (Lars Thomsen) Newsgroups: bionet.biophysics Subject: Freeware for whole cell people. A program that makes a voltage profile. WIN95 32bit Date: Mon, 05 Aug 1996 20:19:01 GMT Organization: Interlynx Lines: 27 Message-ID: <4u8mqp$fna@roger.interlynx.net> NNTP-Posting-Host: ppp45-max1.interlynx.net X-Newsreader: Forte Free Agent 1.0.82 You can find a program called PROFILE at my webpage under "Programs". Profile is a true 32bit WIN95 program and it is a selfextracting zip file (profil.exe), that contains all that you need to do the installation (setup.exe) from your download directory. The program is made in 32bit so the installation includes a uninstaller from Microsoft. The program generates voltage profiles in tbe bitmap format and puts them automatically up in the clipboard. Useful program since MicroCal Origin does not import the voltage profile from PClamp. PROFIL.EXE is 1.6 MB, but you probably already has a lot of the DLL installed so it won't take to much space on your harddrive. find the program at : http://home.interlynx.net/~lthomsen/index.htm (click on PROGRAMS) Lars Thomsen, MSc. PhD. 115 South Oval, Hamilton L8S1R2 Ontario Canada tlf : +1 905 777 0720 fax : +1 905 777 0738 email : lthomsen@interlynx.net homepage : http://home.interlynx.net/~lthomsen/index.htm McMaster University, Intestinal Disease Research Program From owner-biophysics@net.bio.net Tue Aug 06 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!in3.uu.net!ott.istar!istar.net!n3ott.istar!infoshare!passport.ca!news2.insinc.net!roger.interlynx.net!news From: Lars Thomsen Newsgroups: bionet.biophysics Subject: Recipe database - for free - get it here ! Date: Tue, 06 Aug 1996 01:33:13 -0400 Organization: McMaster University, Intestinal Research Disease Programme Lines: 50 Message-ID: <3206D918.E6B@interlynx.net> Reply-To: lthomsen@interlynx.net NNTP-Posting-Host: ppp22-max2.interlynx.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0b5a (Win95; I) Dear Reader I have made a program for Windows95 that basically is a recipe program. The program has two major parts. A chemical database and a solution database. By clicking on the toolbar you can jump from one to another. When you are in the chemical database you can transfer chemicals from a spreedsheet to the current solution, by pressing the right mouse button and chosing "transfer". In the solution window you can add the desired concentration of the chemical and the program will calculate the amount of grams (mg or ml) that you need to make e.g. 1000 ml of the solution. Changing the volume will also change the calculation. The printouts are really nice and they stamped with date and time for the printout. At the printout you will also find the Chemical name, chemical formula and the location of the chemical. This is very useful because I often find myself walking back and forth to the chemical database to find the location of a drug that I forgot where is. This version is freeware, but I would like your comments and ideas for improvement. DOWNLOAD FROM : http://home.interlynx.net/~lthomsen/index.htm Click on the image map for PROGRAMS Program name : CHEMBASE 0.1 Zipped file : CHEM.ZIP contains installation program Requirements : WIN95 and a screen resolution of minimum 800x600 (best viewed in 1024x768) NB The current version must be installed in the directory C:\CHEMBASE to work. The installation program (SETUP.EXE) will by default try to install in C:\PROGRAM FILES\CHEMBASE, but then the program can't find the database. Enjoy it Lars Thomsen -- Lars Thomsen, MSc. PhD. 115 South Oval, Hamilton, L8S1R2 Ontario, Canada tel +1 905 777 0720 fax +1 905 777 0738 email : lthomsen@interlynx.net homepage : http://home.interlynx.net/~lthomsen/index.htm From owner-biophysics@net.bio.net Tue Aug 06 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!gondor!newsfeeder.sdsu.edu!news.sgi.com!news.msfc.nasa.gov!newsfeed.internetmci.com!hunter.premier.net!insync!news.hal-pc.org!pm3-142 From: charliew@hal-pc.org (charliew) Newsgroups: bionet.biophysics Subject: Automotive Engineering/Biomechanics Date: Wed, 07 Aug 96 21:41:38 GMT Organization: Houston Area League of PC Users Lines: 19 Message-ID: <4ub2ii$6p4_001@pm3-142.hal-pc.org> NNTP-Posting-Host: pm3-142.hal-pc.org X-Newsreader: News Xpress Version 1.0 Beta #4 Ladies and gentlemen, I am posting a question for a friend who doesn't have access to a news reader. Please get back with me at your earliest convenience with an answer, or a reference that can give me an answer. My question is: What are the G forces that the human body is subjected to in a low impact collision? I'm looking for information that a human body can still have significant pain resulting from a low impact crash even though there will be little or no damage to the car. I realize that I haven't listed any assumptions regarding seat height, collision angle, height of driver, etc., etc. Therefore, I would appreciate it if you would qualify your answer with the assumptions/constraints that are relevant to a particular situation. Thank you for your time and consideration. From owner-biophysics@net.bio.net Tue Aug 06 23:00:00 1996 Path: biosci!agate!newsgate.duke.edu!news.mathworks.com!newsfeed.internetmci.com!aldehyde.netone.com!news.sprintlink.net!news-fw-12.sprintlink.net!moonbeam.aecom.yu.edu!usenet From: Steve Almo Newsgroups: bionet.xtallography,bionet.biophysics,sci.techniques.xtallography Subject: SDMW detector system for sale Date: 7 Aug 1996 14:15:32 GMT Organization: Albert Einstein College of Medicine Lines: 18 Message-ID: <4ua8e4$1ed@moonbeam.aecom.yu.edu> NNTP-Posting-Host: zonker.bioc.aecom.yu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; IRIX 5.3 IP17) X-URL: news:bionet.xtallography Xref: biosci bionet.xtallography:2787 bionet.biophysics:2202 sci.techniques.xtallography:2958 We are offering an SDMW (Xuong-Hamlin) multiwire system for sale. This system has been under continuous service contract since its purchase ~ 4 years ago, and includes two detectors, helium cones, motorized two-theta table and an AFC-6 goniostat. All offers will be considered. Please respond to Steve Almo Dept of Biochemistry Albert Einstein College of Medicine (718) 430-2746 Phone (718) 430-8565 FAX almo@zugbug.bioc.aecom.yu.edu From owner-biophysics@net.bio.net Tue Aug 06 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news00.sunet.se!sunic!news99.sunet.se!news.uni-c.dk!balder.adm.ku.dk!japs From: Klaus Rytved Newsgroups: bionet.biophysics Subject: Re: Ussing Chambers Date: Tue, 06 Aug 1996 09:56:23 +0200 Organization: BKA AKI KU Lines: 22 Message-ID: <3206FAA7.1BF2@aki.ku.dk> References: <31FC0779.122C@leland.stanford.edu> NNTP-Posting-Host: pc108.aki.ku.dk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 (Win95; I) Michael Lee wrote: > > Anyone know of any good texts of Ussing chambers for transepithelial ion > transport, especially in terms of data interpretation? > > - mlThe following article covers the subject. L.G. Palmer & H. Sackin: Electrophysiological analysis of transepithelial transport. In: The Kidney: Physiology and Pathophysiology. Editors: D.W. Seldin & G. Giebisch. Raven Press, New York. 1992. p. 361-405 Feel free to e-mail me for further information. Regards, Klaus A Rytved / krytved@aki.ku.dk Biochemical Dept. August Krogh Inst. Univ. of Copenhagen. Denmark. http://www.geocities.com/Athens/2720 From owner-biophysics@net.bio.net Wed Aug 07 23:00:00 1996 Path: biosci!agate!howland.erols.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!in3.uu.net!news.emi.com!pauling.wadsworth.org!tivol From: tivol@news.wadsworth.org (William Tivol) Newsgroups: bionet.biophysics Subject: Re: Automotive Engineering/Biomechanics Date: 8 Aug 1996 15:42:40 GMT Organization: Wadsworth Center, NY Health Dept. Lines: 26 Message-ID: <4ud1tg$5km@pauling.wadsworth.org> References: <4ub2ii$6p4_001@pm3-142.hal-pc.org> NNTP-Posting-Host: alcor.wadsworth.org X-Newsreader: TIN [version 1.2 PL2] charliew (charliew@hal-pc.org) wrote: : What are the G forces that the human body is subjected to in : a low impact collision? I'm looking for information that a : human body can still have significant pain resulting from a : low impact crash even though there will be little or no : damage to the car. Dear Charlie, A low-impact collision is usually defined as < 5 mph. A brisk walking pace is ~4 mph. Thus, a low-impact collision can be the equivalent of walking briskly into a wall! Those of us who have done this can assure you that there can be significant pain. The situation in an automobile collision is different in that the seat belt (your friend *was* wearing one, I hope) will constrain the body differently from a collision with a wall, and that the momentum transfer is not as sudden, due to the flexi- bility of the bumper, etc. However, especially if the collision was completely unanticipated, the strain on the body can still be significant, and variables such as body position (was your friend facing straight ahead or turned to another direction, etc.) direction of impact, whether your friend tensed up at the realization that the collision was happening, etc. can influence the amount of pain experienced. The g-force can be calculated if the initial speed and the time for the impact to stop the car are known. At 4 mph and 0.1 sec to decel- erate, this is ~60 ft/sec^2 or ~2g. Yours, Bill Tivol From owner-biophysics@net.bio.net Thu Aug 08 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Petar_Stojadinovic@sequana.com (Petar Stojadinovic) Newsgroups: bionet.biophysics,bionet.genome.chromosomes,bionet.neuroscience,bionet.protista,bionet.software,bionet.software.acedb,bionet.software.gcg,bionet.software.srs,bionet.software.staden,bionet.software.x-plor,bionet.structural-nmr,bionet.toxicology,bionet.users.addresses,bionet.women-in-bio,bionet.xtallography Subject: Clinical Lab Automation & Development, Computational Biology,Biotech Engineering Date: 8 Aug 1996 22:36:25 -0700 Organization: Sequana Therapeutics, Inc. Lines: 13 Sender: biohelp@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Xref: biosci bionet.biophysics:2205 bionet.genome.chromosomes:1303 bionet.neuroscience:15213 bionet.protista:584 bionet.software:16272 bionet.software.acedb:956 bionet.software.gcg:1948 bionet.software.srs:286 bionet.software.staden:221 bionet.software.x-plor:680 bionet.structural-nmr:1434 bionet.toxicology:849 bionet.users.addresses:3079 bionet.women-in-bio:5326 bionet.xtallography:2789 Wanted: Abstracts, Interested members to Join Engineers in Biotechnology Subchapter of Assoc. of Laboratory Automation (ALA) or ICAR in Europe. please send email to : petar@sequana.com PS: Do you know how I may enter a new newsgroup in alt as alt.biotech.engineers? Thank you. Petar Stojadinovic Sequana Therapeutics Inc. Engineering Dept. Phone: 619-6468263 email: petar@sequana.com From owner-biophysics@net.bio.net Thu Aug 08 23:00:00 1996 Path: biosci!agate!newsgate.duke.edu!news-server.ncren.net!concert!lester.appstate.edu!balsam!news From: CRMCKEEL@unca.edu (Cameron McKeel) Newsgroups: bionet.biophysics Subject: Quick Question: Diffusion Coefficient of a couple of Organic Cmpds. Date: 9 Aug 1996 18:27:31 GMT Organization: University of North Carolina at Asheville Lines: 13 Distribution: world Message-ID: <4ufvuj$sun@balsam.unca.edu> NNTP-Posting-Host: uncavx.unca.edu X-News-Reader: VMS NEWS v1.25 Looking for the diffusion coefficients (in air/what temp./1 atm) of: Dichloromethane Styrene 2-methylnapthalene 2,6 Dinitrotoluene Anyone still up on their p-chem? Thanks, Cameron From owner-biophysics@net.bio.net Fri Aug 09 23:00:00 1996 Path: biosci!agate!howland.erols.net!cs.utexas.edu!swrinde!news.sgi.com!news.msfc.nasa.gov!newsfeed.internetmci.com!news.ac.net!news.bconnex.net!news2.insinc.net!roger.interlynx.net!news From: lthomsen@interlynx.net (Lars Thomsen) Newsgroups: bionet.biophysics Subject: Axon file format - how do I read them Date: Fri, 09 Aug 1996 13:26:18 GMT Organization: Interlynx Lines: 27 Message-ID: <4uig55$eeb@roger.interlynx.net> NNTP-Posting-Host: ppp23-max2.interlynx.net X-Newsreader: Forte Free Agent 1.0.82 Dear Reader I am trying to access my PClamp files. I have used all the information available from Axon, however it is not possible to find any meaning in the way the data are stored. I just want to read my data directly from the binary files. The Axon help files explains where the binary offsets are and what the numbers are stored as (float, integers ect). However, using a hexeditor at the specified offsets shows that the information that was supposed to be in that specified location simply either doesn't exist or is totally different from what is specified. I am a big ? Any help will be greatly appreciated Lars Thomsen, MSc. PhD. 115 South Oval, Hamilton L8S1R2 Ontario Canada tlf : +1 905 777 0720 fax : +1 905 777 0738 email : lthomsen@interlynx.net homepage : http://home.interlynx.net/~lthomsen/index.htm McMaster University, Intestinal Disease Research Program From owner-biophysics@net.bio.net Fri Aug 09 23:00:00 1996 Path: biosci!agate!howland.erols.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!news.ac.net!news.bconnex.net!news2.insinc.net!roger.interlynx.net!news From: lthomsen@interlynx.net (Lars Thomsen) Newsgroups: bionet.biophysics Subject: Re: Ussing Chambers Date: Fri, 09 Aug 1996 13:44:04 GMT Organization: Interlynx Lines: 23 Message-ID: <4uih6e$ein@roger.interlynx.net> References: <31FC0779.122C@leland.stanford.edu> NNTP-Posting-Host: ppp23-max2.interlynx.net X-Newsreader: Forte Free Agent 1.0.82 Michael Lee wrote: E Hviid Larsen (1988) NaCl Transport in Amphibia Skin Advances in comparative and enviromental physiology, vol 1 Springer Verlag Berling Heidelberg. In that you will also find other really good references. >Anyone know of any good texts of Ussing chambers for transepithelial ion >transport, especially in terms of data interpretation? > - ml Lars Thomsen, MSc. PhD. 115 South Oval, Hamilton L8S1R2 Ontario Canada tlf : +1 905 777 0720 fax : +1 905 777 0738 email : lthomsen@interlynx.net homepage : http://home.interlynx.net/~lthomsen/index.htm McMaster University, Intestinal Disease Research Program From owner-biophysics@net.bio.net Fri Aug 09 23:00:00 1996 Path: biosci!agate!howland.erols.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!news.ac.net!news.bconnex.net!news2.insinc.net!roger.interlynx.net!news From: lthomsen@interlynx.net (Lars Thomsen) Newsgroups: bionet.biophysics Subject: Chembase ver 0.1 - get it for nothing Date: Fri, 09 Aug 1996 13:26:26 GMT Organization: Interlynx Lines: 53 Message-ID: <4uig5e$eeb@roger.interlynx.net> NNTP-Posting-Host: ppp23-max2.interlynx.net X-Newsreader: Forte Free Agent 1.0.82 Dear Reader I have made a program for Windows95 that basically is a recipe program. The program has two major parts. A chemical database and a solution database. By clicking on the toolbar you can jump from one to another. When you are in the chemical database you can transfer chemicals from a spreedsheet to the current solution, by pressing the right mouse button and chosing "transfer". In the solution window you can add the desired concentration of the chemical and the program will calculate the amount of grams (mg or ml) that you need to make e.g. 1000 ml of the solution. Changing the volume will also change the calculation. The printouts are really nice and they stamped with date and time for the printout. At the printout you will also find the Chemical name, chemical formula and the location of the chemical. This is very useful because I often find myself walking back and forth to the chemical database to find the location of a drug that I forgot where is. This version is freeware, but I would like your comments and ideas for improvement. DOWNLOAD FROM : http://home.interlynx.net/~lthomsen/index.htm Click on the image map for PROGRAMS Program name : CHEMBASE 0.1 Zipped file : CHEM.ZIP contains installation program Requirements : WIN95 and a screen resolution of minimum 800x600 (best viewed in 1024x768) NB The current version must be installed in the directory C:\CHEMBASE to work. The installation program (SETUP.EXE) will by default try to install in C:\PROGRAM FILES\CHEMBASE, but then the program can't find the database. Enjoy it Lars Thomsen Lars Thomsen, MSc. PhD. 115 South Oval, Hamilton L8S1R2 Ontario Canada tlf : +1 905 777 0720 fax : +1 905 777 0738 email : lthomsen@interlynx.net homepage : http://home.interlynx.net/~lthomsen/index.htm McMaster University, Intestinal Disease Research Program From owner-biophysics@net.bio.net Fri Aug 09 23:00:00 1996 Path: biosci!agate!howland.erols.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!in3.uu.net!news00.sunet.se!sunic!news99.sunet.se!news.uni-c.dk!eagle.novo.dk!usenet From: "Sebastian Barg" Newsgroups: bionet.biophysics Subject: file conversion axoscope to pclamp? Date: 9 Aug 1996 15:33:34 GMT Organization: NN Lines: 7 Message-ID: <01bb860b$4eaf63a0$024b4998@lip.novo.dk> NNTP-Posting-Host: lip.novo.dk X-Newsreader: Microsoft Internet News 4.70.1141 Does anyone know about a program for batch file conversion from Axon's .abf (AxoScope) format to .dat (pClamp)? Thanks a lot, Sebastian Barg Islet Cell Physiology, Novo Nordisk Copenhagen DK From owner-biophysics@net.bio.net Fri Aug 09 23:00:00 1996 Path: biosci!agate!spool.mu.edu!uwm.edu!cs.utexas.edu!swrinde!news.sgi.com!news.msfc.nasa.gov!newsfeed.internetmci.com!zdc!nntp04.primenet.com!news.shkoo.com!nntp.primenet.com!ddsw1!news.mcs.net!van-bc!n1van.istar!van.istar!west.istar!ott.istar!istar.net!tor.istar!east.istar!news.nstn.ca!coranto.ucs.mun.ca!plato.ucs.mun.ca!wmfry From: Mark Fry Newsgroups: bionet.biophysics Subject: Re: Freeware for whole cell people. A program that makes a voltage profile. WIN95 32bit Date: Sat, 10 Aug 1996 15:45:05 -0230 Organization: Memorial University of Newfoundland Lines: 24 Message-ID: References: <4u8mqp$fna@roger.interlynx.net> NNTP-Posting-Host: plato.ucs.mun.ca Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: wmfry@plato.ucs.mun.ca In-Reply-To: <4u8mqp$fna@roger.interlynx.net> On Mon, 5 Aug 1996, Lars Thomsen wrote: some stuff here: > > The program generates voltage profiles in tbe bitmap format and puts > them automatically up in the clipboard. Useful program since MicroCal > Origin does not import the voltage profile from PClamp. Microcal Origin certainly WILL import voltage profile from pclamp. The import module comes on a seperate diskette and is specifically designed to import pclamp 5.5. I don't know how much this module costs, but my advice is to contact Origin software and ask: whatever the cost, it is money well spent: Pclamp software is very fine stuff, and microcal origin is by far the best statistical package i've ever seen. All the usual disclaimers apply. Mark Fry Basic Medical Sciences, Faculty of Med Memorial University of Newfoundland Canada From owner-biophysics@net.bio.net Sun Aug 11 23:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!newsxfer2.itd.umich.edu!agate!dog.ee.lbl.gov!news.cs.utah.edu!swen.emba.uvm.edu!newsmaster From: guilford@salus.med.uvm.edu (William H. Guilford) Newsgroups: bionet.biophysics,bionet.cellbiol Subject: Large liposomes Date: Fri, 09 Aug 1996 17:58:00 GMT Organization: EMBA Computer Facility, The University of Vermont Lines: 30 Message-ID: <4ufuf1$61g@swen.emba.uvm.edu> Reply-To: guilford@salus.med.uvm.edu NNTP-Posting-Host: torino.med.uvm.edu X-Newsreader: Forte Free Agent 1.0.82 Xref: biosci bionet.biophysics:2212 bionet.cellbiol:5249 Folks: I recently saw an article describing the production of very large (>1 um) liposomes, but have misplaced it. I can't remember the journal or the author, and have been unable to find it on Medline (perhaps because it was recently published). Can anyone direct me to this article? Thanks in advance! - Bill G. ___ _ ____ _ ___ / \__/ \__/ \__/ \__/ \ | _|@ @ __ | \________/ | | \________/ __/ _/ William H.O. Guilford /) (o _/ Molecular Physiology and Biophysics \____/ University of Vermont Burlington, VT 05405 Vermont - We got moose. ----------------------------------------------------------------------------- mail: guilford@salus.med.uvm.edu Talk: drbill@torino.med.uvm.edu WWW : http://salus.med.uvm.edu/~guilford/home.html Phn : (802) 656-3492 Fax : (802) 656-0747 ---------------------------------------------------------------------------- From owner-biophysics@net.bio.net Sun Aug 11 23:00:00 1996 Path: biosci!rutgers!gatech!usenet.eel.ufl.edu!news.ultranet.com!homer.alpha.net!uwm.edu!newsfeed.internetmci.com!news.intercall.com!news.sprintlink.net!news-pen-4.sprintlink.net!ratty.wolfe.net!usenet From: Dan Brown Newsgroups: bionet.biophysics Subject: Re: file conversion axoscope to pclamp? Date: Mon, 12 Aug 1996 08:29:12 -0700 Organization: SKALAR Instruments, Inc. Lines: 38 Message-ID: <320F4DC8.7FE@skalar.com> References: <01bb860b$4eaf63a0$024b4998@lip.novo.dk> NNTP-Posting-Host: 206.159.30.20 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (WinNT; I) To: Sebastian Barg Sebastian Barg wrote: > > Does anyone know about a program for batch file conversion from Axon's .abf > (AxoScope) format to .dat (pClamp)? Sebastian, As far as I know, the two formats are the same. We have software products that read both without any trouble. (TAC for single-channel analysis and Data Access for data file reading pCLAMP/AxoScope files in Excel, SigmaPlot, Visual Basic, and IGOR Pro.) The differences of which I am aware are: - AxoScope files contain no stimulus information (in this sense they are like files produced by Clampex). The Fetchex part of pCLAMP can produce files with stimulus information. - AxoScope files may contain tags that are not present in files produced by programs in the pCLAMP package. I am not aware if pCLAMP programs can read such files. - The creator information in the header is slightly different between the two packages, just because each program puts its own name in the header. What difficulties are you having reading AxoScope files in pCLAMP? -- Dan -- Dan Brown SKALAR Instruments, Inc. Seattle Phone: (206) 782-8862 Web: http://www.skalar.com From owner-biophysics@net.bio.net Sun Aug 11 23:00:00 1996 Path: biosci!rutgers!uwm.edu!newsfeed.internetmci.com!newsreader.sprintlink.net!news.sprintlink.net!news-pen-14.sprintlink.net!ratty.wolfe.net!usenet From: Dan Brown Newsgroups: bionet.biophysics Subject: Re: Axon file format - how do I read them Date: Mon, 12 Aug 1996 08:33:31 -0700 Organization: SKALAR Instruments, Inc. Lines: 32 Message-ID: <320F4ECB.6423@skalar.com> References: <4uig55$eeb@roger.interlynx.net> NNTP-Posting-Host: 206.159.30.20 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (WinNT; I) Lars Thomsen wrote: > I am trying to access my PClamp files. I have used all the information > available from Axon, however it is not possible to find any meaning in > the way the data are stored. Lars, We have file readers for the pCLAMP files. We spent some time getting them to work correctly. I believe that we found all the information in the Axon Instruments documentation to be correct, but not as obvious as one might like. I see from your other postings that you are working in Visual Basic. We have a product called Data Access that provides our file readers for Axon Instruments pCLAMP, HEKA Pulse, and SKALAR Acquire files in a form that can be directly called from Visual Basic (among others - we also support Excel, SigmaPlot, and IGOR Pro). This is a commercial product, and we intend to support it going forward as the file formats are updated. Please contact me if you have any questions about this product. -- Dan -- Dan Brown SKALAR Instruments, Inc. Seattle Phone: (206) 782-8862 Web: http://www.skalar.com From owner-biophysics@net.bio.net Sun Aug 11 23:00:00 1996 Path: biosci!GUITAR.ROCKEFELLER.EDU!ilya From: ilya@GUITAR.ROCKEFELLER.EDU (Ilya Vakser) Newsgroups: bionet.biophysics Subject: Announcement - protein docking program GRAMM Date: 12 Aug 1996 15:41:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 92 Sender: daemon@net.bio.net Distribution: world Message-ID: <320FB45A.15FB@guitar.rockefeller.edu> NNTP-Posting-Host: net.bio.net ----------- GRAMM v1.03 ----------- This note is to announce the availability of GRAMM (Global RAnge Molecular Matching) software. GRAMM is a program for protein docking. To predict the structure of a complex, it requires only the atomic coordinates of the two molecules (no information about the binding sites is needed). The program performs an exhaustive 6-dimensional search through the relative translations and rotations of the molecules. The molecular pairs may be: two proteins, a protein and a smaller compound, two transmembrane (TM) helices, etc. GRAMM may be used for high- resolution molecules, for inaccurate structures (where only the gross structural features are known), in cases of large conformational changes, etc. The Global Range Molecular Matching (GRAMM) methodology is an empirical approach to smoothing the intermolecular energy function by changing the range of the atom-atom potentials. The technique allows to locate the area of the global minimum of intermolecular energy for structures of different accuracy. The quality of the prediction depends on the accuracy of the structures. Thus, the docking of high-resolution structures with small conformational changes yields an accurate prediction, while the docking of ultralow-resolution structures will give only the gross features of the complex. _________________ I am making GRAMM publicly available following a number of requests from different labs. I would like to make it clear, however, that both the methodology and the program, at present, are in the process of active development and validation, especially in the area of the low-resolution docking, and have to be viewed like that. The program is free. However, I would expect proper references. I will also appreciate bug reports. To get GRAMM, send the request to ilya@guitar.rockefeller.edu. The GRAMM site on the Web is http://guitar.rockefeller.edu/. GRAMM was created during my stay in the Weizmann Institute (1991-1993), in the Washington University (1993-1995), and in the Rockefeller University (1995-1996). I deeply appreciate the assistance of my colleagues in all these institutions (especially, Profs. Ephraim Katchalski-Katzir, Garland Marshall, and Andrej Sali). Platforms Presently, GRAMM is compiled on SGI R4000, SGI R4400, SGI R8000, and SGI R10000 Unix workstations. In the near future I will expand this list, so check the GRAMM site for the updates. Interestingly, GRAMM also works on a PC platform under Windows95 (the performance on P5-120 with 16 MB RAM is only two times slower than on SGI 250 MHZ Indigo2 R4400). Papers on GRAMM methodology * E. Katchalski-Katzir, I. Shariv, M. Eisenstein, A. A. Friesem, C. Aflalo, I. A. Vakser, 1992, Molecular surface recognition: Determination of geometric fit between proteins and their ligands by correlation techniques, Proc. Natl. Acad. Sci. USA, 89, 2195-2199. (Basic algorithm of protein recognition by correlation technique with Fast Fourier transform. High-resolution 'geometric' docking). * I. A. Vakser, C. Aflalo, 1994, Hydrophobic docking: A proposed enhancement to molecular recognition techniques, Proteins, 20, 320-329. (High-resolution 'hydrophobic' docking). * I. A. Vakser, G. V. Nikiforovich, 1995a, Protein docking in the absence of detailed molecular structures, in: Methods in Protein Structure Analysis (M. Z. Atassi & E. Appella, eds.), Plenum Press, New York, pp. 505-514. * I. A. Vakser, 1995b, Protein docking for low-resolution structures, Protein Eng., 8, 371- 377. ('Low-resolution' protein docking). * I. A. Vakser, 1996a, Long-distance potentials: An approach to the multiple-minima problem in ligand-receptor interaction, Protein Eng., 9, 37-41. (Interpretation of the low-resolution docking in terms of energy potentials). * I. A. Vakser, 1996b, Low-resolution docking: Prediction of complexes for underdetermined structures, Biopolymers, in press (39, No.3). (Validation of the low-resolution docking). * I. A. Vakser, 1996c, Main-chain complementarity in protein-protein recognition, Protein Eng., in press (9, No.7). (Docking of Ca structures). ------------------------------------------------------------------ Ilya A. Vakser The Rockefeller University, Box 270 1230 York Avenue, New York, NY 10021 Phone: (212) 327-7206; Fax: (212) 327-7540 Email: ilya@guitar.rockefeller.edu ------------------------------------------------------------------ From owner-biophysics@net.bio.net Tue Aug 13 23:00:00 1996 Path: biosci!rutgers!csn!nntp-xfer-1.csn.net!carbon!night.primate.wisc.edu!sdd.hp.com!swrinde!howland.erols.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!netnews.nwnet.net!news.u.washington.edu!uw-beaver!cornellcs!newsstand.cit.cornell.edu!usenet From: Tom Chou Newsgroups: bionet.biophysics,bionet.molec-model Subject: (no subject) Date: 13 Aug 1996 21:29:52 GMT Organization: LASSP, Cornell Univ. Lines: 10 Sender: tc42@cornell.edu (Verified) Message-ID: <4uqs4g$pti@newsstand.cit.cornell.edu> NNTP-Posting-Host: jaguar.msc.cornell.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; AIX 2) X-URL: news:bionet.biophysics Xref: biosci bionet.biophysics:2216 bionet.molec-model:1106 Hi all, Does anyone know if any pumps, antiporters, atpases, or flagellar motors have been X-ray X-stal structured? Thanks, Tom From owner-biophysics@net.bio.net Tue Aug 13 23:00:00 1996 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!howland.erols.net!netcom.com!tday.slip.netcom.com!user From: tday@netcom.com (Tony Day) Newsgroups: bionet.biophysics,,bionet.molbio.proteins Subject: Tb3+ binding and fluorescence anybody? Date: Wed, 14 Aug 1996 08:31:07 -0700 Organization: NETCOM On-Line Communications Services Lines: 15 Message-ID: NNTP-Posting-Host: tday.slip.netcom.com Xref: biosci bionet.biophysics:2217 bionet.molbio.proteins:8508 I have been working with an enzyme of about 55kD. I know that this protein binds Ca2+, and I can show that it binds at least 2M of Tb3+ by thermal inactivation ( a plot of inactivation rate constant vs [Tb3+] fits perfectly to 2 site binding). However I can see no fluorescence enhancement! Using 6mg/ml protein, emission at 545nm (5nm slit width) I see no change in the excitation spectrum with added Tb (~2mM). Does anyone have any idea whether I am doing something wrong, or just unlucky? Many thanks in advance. Please reply by email to tday@genencor.com Tony From owner-biophysics@net.bio.net Tue Aug 13 23:00:00 1996 Path: biosci!rutgers!csn!nntp-xfer-1.csn.net!carbon!night.primate.wisc.edu!caen!spool.mu.edu!news.sgi.com!swrinde!howland.erols.net!surfnet.nl!swsbe6.switch.ch!scsing.switch.ch!news.belwue.de!newsserv.zdv.uni-tuebingen.de!news From: nollert@gen.mpib-tuebingen.mpg.de (Peter Nollert) Newsgroups: bionet.biophysics Subject: Re: cationic lipid? Date: 14 Aug 1996 18:55:49 GMT Organization: MPI for Biology Lines: 29 Message-ID: <4ut7fl$4ed@newsserv.zdv.uni-tuebingen.de> References: NNTP-Posting-Host: @davis.mpib-tuebingen.mpg.de X-Newsreader: WinVN 0.92.6+ In article , bijt@zeus.bris.ac.uk (SH. Doole) says: > >Hi all, > >does anybody know manufacturer or retailer of N-[1-(2,3-dioleyloxy)propyl]- >N,N,N-trimethyleammonium chloride (DOTMA)? I know Gibco sells the vesicles >as a kit, but it's too expensive. > >Or, any cationic lipid may do the job. > >TIA > >Jiro Hi Jiro, when I want to add positive charge to a membrane I add Dimethyldioctadecylammonium bromide. It's not a *real* lipid, but the same is true for DOTMA. Sigma sells it, it's cheap. Peter Nollert Dept Membrane Biochemistry MPI Biology Tuebingen, Germany nollert@mpib-tuebingen.mpg.de From owner-biophysics@net.bio.net Tue Aug 13 23:00:00 1996 Path: biosci!rutgers!gatech!usenet.eel.ufl.edu!spool.mu.edu!news.sgi.com!swrinde!howland.erols.net!agate!newsgate.duke.edu!godot.cc.duq.edu!usenet From: "Frank R. Gorga" Newsgroups: bionet.biophysics,,bionet.molbio.proteins Subject: Re: Tb3+ binding and fluorescence anybody? Date: Wed, 14 Aug 1996 14:12:21 -0700 Organization: Duquesne University Lines: 39 Message-ID: <32124135.477A@next.duq.edu> References: NNTP-Posting-Host: phosphorus.chemistry.duq.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) Xref: biosci bionet.biophysics:2219 bionet.molbio.proteins:8509 Tony Day wrote: > > I have been working with an enzyme of about 55kD. I know that this > protein binds Ca2+, and I can show that it binds at least 2M of Tb3+ by > thermal inactivation ( a plot of inactivation rate constant vs [Tb3+] fits > perfectly to 2 site binding). However I can see no fluorescence > enhancement! Using 6mg/ml protein, emission at 545nm (5nm slit width) I > see no change in the excitation spectrum with added Tb (~2mM). > > Does anyone have any idea whether I am doing something wrong, or just unlucky? > > Many thanks in advance. > > Please reply by email to tday@genencor.com > > Tony Not having worked with Tb3+ fluoresence personally, I am not sure what type of change you are expecting, but the following thought did occur to me: 6 mg/mL of a 55 kDa protein works out to about 0.1 mM protein. If 2 mM Tb3+ is enough to saturate both sites, then the [Tb3+] bound (i.e the maximum one could expect) will be ~ 0.2 mM, or 10% of the total Tb3+ present. Are you sure that you would be able to detect a signal this small over the "background" of the free (unbound) Tb3+??? Just a thought... hope it helps! --- F. R. Gorga *********************************** Frank R. Gorga, Ph.D. Dept. of Chemistry & Biochemistry Duquesne University Pittsburgh, PA 15282 412-396-5858 / 412-396-5683 (fax) gorga@next.duq.edu From owner-biophysics@net.bio.net Tue Aug 13 23:00:00 1996 Path: biosci!rutgers!gatech!usenet.eel.ufl.edu!spool.mu.edu!news.sgi.com!swrinde!howland.erols.net!news.cac.psu.edu!news.cse.psu.edu!news.eecs.nwu.edu!newsfeed.acns.nwu.edu!news.luc.edu!orion!scukier From: scukier@orion.it.luc.edu (Sam Cukierman) Newsgroups: bionet.biophysics Subject: cationic lipid Date: 14 Aug 1996 13:29:09 GMT Organization: Loyola University Chicago Lines: 1 Message-ID: <4uskb5$7mi@artemis.it.luc.edu> NNTP-Posting-Host: 147.126.1.9 NNTP-Posting-User: scukier X-Newsreader: TIN [version 1.2 PL2] Have you tried KODAK? They have some cationic lipids. From owner-biophysics@net.bio.net Wed Aug 14 23:00:00 1996 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!enews.sgi.com!news.sgi.com!swrinde!howland.erols.net!newsxfer2.itd.umich.edu!portc01.blue.aol.com!newsstand.cit.cornell.edu!usenet From: Tom Chou Newsgroups: bionet.biophysics,bionet.molec-model Subject: MD in Cambridge, UK? Date: 15 Aug 1996 14:43:27 GMT Organization: LASSP, Cornell Univ. Lines: 12 Sender: tc42@cornell.edu (Verified) Message-ID: <4uvd2f$gg9@newsstand.cit.cornell.edu> NNTP-Posting-Host: jaguar.msc.cornell.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; AIX 2) X-URL: news:bionet.biophysics Xref: biosci bionet.biophysics:2222 bionet.molec-model:1109 Hello, Does anyone know of people doing biological modelling/molecular dynamics/ crystal structure in Cambridge UK. I'll be going there and would like tohave a list of people doing similar research..... Thanks, Tom From owner-biophysics@net.bio.net Wed Aug 14 23:00:00 1996 Path: biosci!rutgers!news.sgi.com!news-res.gsl.net!news.gsl.net!hunter.premier.net!news.thenet.net!uunet!in3.uu.net!news.zNET.net!news From: laradex3@sj.znet.com (Larry Adams) Newsgroups: bionet.biophysics Subject: Recommend Intro. Texts for Biophysics? Date: 15 Aug 1996 03:42:16 GMT Organization: none Lines: 12 Message-ID: <4uu6ao$6m9@news> NNTP-Posting-Host: capts-26.znet.com Mime-Version: 1.0 X-Newsreader: WinVN 0.93.14 I have multiple associate degrees and am interested in biophysics. Can you recommend some texts or other resources that would introduce me to this subject? (I have access to the science library at the University of California at Santa Cruz). Thanks. Larry Adams laradex3@sj.znet.com From owner-biophysics@net.bio.net Wed Aug 14 23:00:00 1996 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!howland.erols.net!agate!newsgate.duke.edu!usenet From: Kevin Olbrich Newsgroups: bionet.biophysics Subject: Re: cationic lipid? Date: Thu, 15 Aug 1996 16:20:29 -0400 Organization: Duke University Lines: 41 Message-ID: <3213868D.7A22@acpub.duke.edu> References: Reply-To: kco1@acpub.duke.edu NNTP-Posting-Host: vangogh.egr.duke.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) To: "SH. Doole" SH. Doole wrote: > > Hi all, > > does anybody know manufacturer or retailer of N-[1-(2,3-dioleyloxy)propyl]- > N,N,N-trimethyleammonium chloride (DOTMA)? I know Gibco sells the vesicles > as a kit, but it's too expensive. > > Or, any cationic lipid may do the job. > > TIA > > Jiro > > ---- > > Jiro Takei > Dept of Biochem > Univ of Bristol > j.takei@bris.ac.uk Try Avanti Polar Lipids, they have a variety of cationic lipids. -- ================================================ Kevin Olbrich CCBE NIH Fellow Dept. of Mechanical Engineering Center for Cell and Biosurface Engineering Duke University, Box 90300 Durham, NC 27708-0300 Phone: (919) 660-5357 Fax: (919) 660-5362 mailto:kco1@acpub.duke.edu WWW: http://www.duke.edu/~kco1 'For every phenomenon, however complex, someone will eventually come up with a simple and elegant theory. This theory will be wrong.' --Rothchild's Rule From owner-biophysics@net.bio.net Thu Aug 15 23:00:00 1996 Newsgroups: bionet.biophysics Path: biosci!rutgers!uwm.edu!spool.mu.edu!usenet.eel.ufl.edu!warwick!bris.ac.uk!not-for-mail From: bijt@zeus.bris.ac.uk (J. Takei) Subject: Re: cationic lipid X-Nntp-Posting-Host: zeus.bris.ac.uk Message-ID: Sender: usenet@fsa.bris.ac.uk (Usenet) Organization: University of Bristol, England X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] References: <4uskb5$7mi@artemis.it.luc.edu> Date: Fri, 16 Aug 1996 10:29:00 GMT Lines: 17 Sam Cukierman (scukier@orion.it.luc.edu) wrote: : Have you tried KODAK? They have some cationic lipids. Hi Sam, Thanks for the info. Another request for you. Do you have contact address for KODAK, e-mail would be better. Thanks again. Jiro j.takei@bris.ac.uk Dept of Biochem. Univ. of Bristol UK From owner-biophysics@net.bio.net Thu Aug 15 23:00:00 1996 Path: biosci!rutgers!uwm.edu!spool.mu.edu!newshub.tc.umn.edu!mr.net!nntp04.primenet.com!news.shkoo.com!nntp.primenet.com!news.cais.net!nntp.uio.no!nntp-oslo.UNINETT.no!nntp-trd.UNINETT.no!daresbury!not-for-mail From: bmfriedm@weizmann.weizmann.ac.il (Peter Friedman) Newsgroups: bionet.biophysics Subject: Re: cationic lipid? Date: 16 Aug 1996 18:35:08 +0100 Lines: 36 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4v2bgc$c9n@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk Try Avanti Polar Lipids: tel: (205)-663-2494 fax: (205)-663-0756 >Hi all, > >does anybody know manufacturer or retailer of N-[1-(2,3-dioleyloxy)propyl]- >N,N,N-trimethyleammonium chloride (DOTMA)? I know Gibco sells the vesicles >as a kit, but it's too expensive. > >Or, any cationic lipid may do the job. > >TIA > >Jiro > >---- > >Jiro Takei >Dept of Biochem >Univ of Bristol >j.takei@bris.ac.uk -=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Peter Friedman Dept. of Membrane Research & Biophysics The Weizmann Institute of Science Rehovot 76100, Israel tel : +972-8-9343675 fax : +972-8-9344112 E-mail : bmfriedm@weizmann.weizmann.ac.il bmfriedm@wiccmail.weizmann.ac.il From owner-biophysics@net.bio.net Fri Aug 16 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.biophysics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Aug 1996 02:00:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199608170900.CAA21932@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-biophysics@net.bio.net Sun Aug 18 23:00:00 1996 Path: biosci!TTUHSC.EDU!phyan From: phyan@TTUHSC.EDU (Alan Neely) Newsgroups: bionet.biophysics Subject: Postdoctoral Position Date: 19 Aug 1996 09:23:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 39 Sender: daemon@net.bio.net Distribution: world Message-ID: <199608191623.JAA26817@net.bio.net> NNTP-Posting-Host: net.bio.net OSITION OPEN: Postdoctoral Fellowship (Ion channel gating) INSTITUTION: Texas Tech University Health Sciences ADDRESS: Department of Physiology 3601 4th Street Lubbock, TX 79430 CONTACT PERSON: Alan Neely, Ph.D. PHONE: (806) 743-2089 FAX: (806) 743-1512 EMAIL: phyan@ttuhsc.edu : SALARY: Equal or better than NIH guidelines depending on qualifications JOB DESCRIPTION: To study structure and function of ion-channels. Current research is on the mechanisms coupling voltage sensing to channel opening and the role of ion-channel's auxiliary subunits on function and expression. The laboratory combines molecular biology with electrophysiology. The latter include the recently developped cut-open oocytes technique for the recording of gating currents. JOB REQUIREMENTS: Experience in either molecular-biology or electrophysiology and a strong commitement to research. The candidate is expected to perfect skills in both techniques. APPLICATION REQUIREMENTS: Curriculum Vitae along with a brief description of research interests. APPLICATION DEADLINE: No later than December 1996 Date Posted: 8/6/96 . P.S. For additional information check: http://phy025.lubb.ttuhsc.edu/Neely/Neely.htm From owner-biophysics@net.bio.net Mon Aug 19 23:00:00 1996 Path: biosci!rutgers!uwm.edu!chi-news.cic.net!ddsw1!news.mcs.net!nntp04.primenet.com!nntp.primenet.com!mr.net!news.ios.com!nebo.vii.com!xmission!news.cc.utah.edu!news From: Bob Dutnall Newsgroups: bionet.biophysics,bionet.molec-model Subject: Re: MD in Cambridge, UK? Date: Mon, 19 Aug 1996 18:37:39 -0600 Organization: University of Utah Computer Center Lines: 10 Message-ID: <321908D3.4CBD@snowbird.med.utah.edu> References: <4uvd2f$gg9@newsstand.cit.cornell.edu> NNTP-Posting-Host: biochem-107.med.utah.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) Xref: biosci bionet.biophysics:2229 bionet.molec-model:1112 You could try contacting Dr. Arthur Lesk or Dr. Cyrus Chothia at the MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK. They both work on analysis of protein structures and there are also several crystallographers at the same lab. Their e-mail are: Arthur Lesk: aml2@mrc-lmb.cam.ac.uk Cyrus Chothia: chc1@mrc-lmb.cam.ac.uk From owner-biophysics@net.bio.net Mon Aug 19 23:00:00 1996 Path: biosci!rutgers!uwm.edu!news-res.gsl.net!news.gsl.net!EU.net!Norway.EU.net!online.no!nntp-oslo.UNINETT.no!nntp-trd.UNINETT.no!daresbury!lyra.csx.cam.ac.uk!moose.bioc.cam.ac.uk!user From: nef1002@cus.cam.ac.uk (Nick Fisher) Newsgroups: bionet.biophysics,bionet.molec-model Subject: Re: MD in Cambridge, UK? Date: Tue, 20 Aug 1996 14:40:51 +0100 Organization: Dept. of Biochemistry, University of Cambridge Lines: 24 Message-ID: References: <4uvd2f$gg9@newsstand.cit.cornell.edu> NNTP-Posting-Host: moose.bioc.cam.ac.uk Xref: biosci bionet.biophysics:2230 bionet.molec-model:1114 In article <4uvd2f$gg9@newsstand.cit.cornell.edu>, Tom Chou wrote: > Hello, > > Does anyone know of people doing biological modelling/molecular dynamics/ > crystal structure in Cambridge UK. I'll be going there and > would like tohave a list of people doing similar research..... > > > Thanks, > > > Tom Take a look at the Biological Sciences homepage here. It's http://www.bio.cam.ac.uk/ Nick Fisher Biochem Dept. Cambridge Univ. -- From owner-biophysics@net.bio.net Tue Aug 20 23:00:00 1996 Path: biosci!rutgers!uwm.edu!news-res.gsl.net!news.gsl.net!usenet.eel.ufl.edu!newsfeed.internetmci.com!in3.uu.net!news.pc.centuryinter.net!usenet From: "Tony Purkal" Newsgroups: bionet.biophysics Subject: Shareware & Freeware Physics Programs Date: 21 Aug 1996 02:01:33 GMT Organization: Century Internet Lines: 5 Message-ID: <01bb8f07$917927c0$1bb741ce@cen03887> NNTP-Posting-Host: anxp17.ga.centuryinter.net X-Newsreader: Microsoft Internet News 4.70.1155 I am a junior in high school. Could you find some shareware and freeware physics program? If you find some please post the name of the program, and a short description if possible, and their internet addresse. Any efforts will be greatly appreciated! From owner-biophysics@net.bio.net Wed Aug 21 23:00:00 1996 Path: biosci!rutgers!csn!nntp-xfer-1.csn.net!csn!nntp-xfer-2.csn.net!yuma!purdue!oitnews.harvard.edu!das-news2.harvard.edu!news4.ner.bbnplanet.net!cam-news-hub1.bbnplanet.com!uunet!in3.uu.net!news.net1.net!news From: fergyee@net1.net (Irene Makris) Newsgroups: bionet.biophysics Subject: pituitary tumors chromophobe adenomas Date: Wed, 21 Aug 1996 21:17:31 GMT Organization: Net One Lines: 9 Message-ID: <4vfmjf$qg1@news.net1.net> NNTP-Posting-Host: ppp068.net1.net X-Newsreader: Forte Free Agent 1.0.82 urgently need information on non-surgical and non-radiation treament of chromophobe adenomas of pituitary gland. thanks irene makris 8/21/96 e mail fergyee@net1.net From owner-biophysics@net.bio.net Wed Aug 21 23:00:00 1996 Path: biosci!rutgers!csn!nntp-xfer-1.csn.net!csn!nntp-xfer-2.csn.net!yuma!purdue!haven.umd.edu!news.ums.edu!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!howland.erols.net!newsfeed.internetmci.com!in3.uu.net!01-newsfeed.univie.ac.at!03-newsfeed.univie.ac.at!fstgal00.tu-graz.ac.at!not-for-mail From: Roger Hage Newsgroups: bionet.biophysics Subject: RBC Aggregation & Rouleau Formation Date: Wed, 21 Aug 1996 17:46:34 +0200 Organization: Graz University of Technology, Austria Lines: 28 Message-ID: <321B2F5A.7836@ping.at> NNTP-Posting-Host: hager.avl.co.at Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (Win16; I) Does anyone has a hint on the following topic: The velocity of in-vitro Red Blood Cell (anticoagulated whole blood Hct=0,44) aggregation after flow stop in 0.1mm cuvette? When do the cells aggregate and what are the conditions for encountering single RBCs? Your comments and ideas are appreciated. Regards Roger ,,, /'^'\ ( o o ) --------------------------------------------------oOOO--(_)--OOOo-- DI Roger Hage Annenstrasse 14, A-8020 Graz / AUSTRIA WWW: http://www.ping.at/members/rhage .oooO E- mailto:RHAGE@ping.at ( ) Oooo. ----------------------------------------------------\ (----( )--- \_) ) / (_/ From owner-biophysics@net.bio.net Wed Aug 21 23:00:00 1996 Path: biosci!rutgers!csn!nntp-xfer-1.csn.net!dimensional.com!imci2!imci3!newsfeed.internetmci.com!in2.uu.net!newsgate.watson.ibm.com!newsjunkie.ans.net!newsfeeds.ans.net!lantana.singnet.com.sg!violet.singnet.com.sg!temasek.teleview.com.sg!usenet From: "WANG CHERN HOE" Newsgroups: bionet.biophysics Subject: ceramide, sphingosine, lipid A Date: 21 Aug 1996 16:50:49 GMT Organization: NUS Lines: 16 Message-ID: <01bb8f6b$1bdd9640$992915a5@wangch> NNTP-Posting-Host: xyplex2313.teleview.com.sg X-Newsreader: Microsoft Internet News 4.70.1155 Hi all, Does anybody know whether there are any NMR or X-ray structures for these compounds : ceramide sphingosine lipid A lipopolysaccharide Thanks! -- wangch@teleview.com.sg Wang Chern Hoe National University of Singapore From owner-biophysics@net.bio.net Wed Aug 21 23:00:00 1996 Path: biosci!rutgers!uwm.edu!news-res.gsl.net!news.gsl.net!nntp.coast.net!news.dacom.co.kr!news.kreonet.re.kr!news.postech.ac.kr!galaxy.postech.ac.kr!ppjun From: ppjun@galaxy.postech.ac.kr (Park Byung Jun) Newsgroups: bionet.biophysics Subject: NEED INFO: Membrane Viscoelasticity ?? Date: 22 Aug 1996 07:22:32 GMT Organization: POSTECH, Pohang, Korea Lines: 17 Message-ID: <4vh1ro$brs@news.postech.ac.kr> NNTP-Posting-Host: galaxy.postech.ac.kr X-Newsreader: TIN [version 1.2 PL1] Biomembrane is known as Non-Newtonian fluid generally. Hence, It does have viscosity and elasticity simultaneously according to the time scale of applied perturbations. I'm now studying the mechanical properties of cell membrane in terms of theoretical physics, and want to know some parameter values. Where can I find the membrane parameters such as shear modulus, relaxation time etc. for erythrocite or other cell membranes? If there is anyone who know this, please let me know. Thank you very much in advance. P.J. Park Dept. of Physics, POSTECH Pohang 790-784 KOREA E-mail: ppjun@galaxy.postech.ac.kr From owner-biophysics@net.bio.net Wed Aug 21 23:00:00 1996 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!newsserver.jvnc.net!crick.bms.com!greend.bms.com!user From: Green_david_w.prilvms3@msmail.bms.com (David W. Green) Newsgroups: bionet.biophysics Subject: bongkrekic acid Date: Wed, 21 Aug 1996 11:08:18 -0400 Organization: Bristol-Myers Squibb Lines: 7 Message-ID: NNTP-Posting-Host: greend.bms.com Can anyone help me with finding a commercial source of bongkrekic acid? Thanks David W. Green Bristol-Myers Squibb Pharmaceutical Research Institute Princeton, NJ 08543 From owner-biophysics@net.bio.net Wed Aug 21 23:00:00 1996 Newsgroups: bionet.biophysics Path: biosci!daresbury!lyra.csx.cam.ac.uk!warwick!bris.ac.uk!not-for-mail From: bijt@zeus.bris.ac.uk (SH. Doole) Subject: cationic lipid? X-Nntp-Posting-Host: zeus.bris.ac.uk Message-ID: Sender: usenet@fsa.bris.ac.uk (Usenet) Organization: University of Bristol, England X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Date: Tue, 13 Aug 1996 13:10:01 GMT Lines: 20 Hi all, does anybody know manufacturer or retailer of N-[1-(2,3-dioleyloxy)propyl]- N,N,N-trimethyleammonium chloride (DOTMA)? I know Gibco sells the vesicles as a kit, but it's too expensive. Or, any cationic lipid may do the job. TIA Jiro ---- Jiro Takei Dept of Biochem Univ of Bristol j.takei@bris.ac.uk From owner-biophysics@net.bio.net Wed Aug 21 23:00:00 1996 Path: biosci!rutgers!mcrcr6!cmcl2!news.sprintlink.net!news-stk-200.sprintlink.net!news-res.gsl.net!news.gsl.net!hunter.premier.net!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news Newsgroups: bionet.biology.cardiovascular,bionet.biophysics,bionet.general,bionet.molbio.ageing,bionet.neuroscience Subject: ACTIVITY METER FOR LAB ANIMALS Message-ID: <4vf3o2$mi8$1@mhafn.production.compuserve.com> From: JAN CZEKAJEWSKI <75144.2413@CompuServe.COM> Date: 21 Aug 1996 13:42:26 GMT Organization: Columbus Instruments Lines: 20 Xref: biosci bionet.biology.cardiovascular:1137 bionet.biophysics:2236 bionet.general:23020 bionet.molbio.ageing:2951 bionet.neuroscience:15385 The behavioral change in response to drug in laboratory animals can be assessed partly by monitoring through the "Auto-Track" animal Activity Meter. The system has an interface to IBMPC/AT compatible computers that provides complete automation of data collection. It also provides 'zoning' capability and monitors subject entries and latencies for up to multiple user-defined cage areas. The following features are very unique for Auto-Track. 1. Monitors upto 16 cages simultaneously. 2. Distance travelled and pattern of movement data. 3. Separates ambulatory activity from total activity. 4. Provides zone monitoring capability. 5. Can be optionally equipped for animal rotation monitor. 6. Computer storage of processed and raw data. 7. 15 infra-red photocell per axis. 8. Primary vertical sensor for rearing activity and secondary vertical sensor is for jumping. If you need further information, please e-mail your postal address. I will be happy to mail you all information. From owner-biophysics@net.bio.net Wed Aug 21 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!gondor!newsfeeder.sdsu.edu!news.sgi.com!spool.mu.edu!usenet.eel.ufl.edu!tank.news.pipex.net!pipex!dish.news.pipex.net!pipex!news.hol.gr!dmbbs5 From: restefel@hol.gr (Eleftheropulos George) Newsgroups: bionet.biophysics Subject: TOYR KIND HELP IS NEEDED Date: 23 Aug 1996 03:05:53 GMT Organization: Hellas On Line Lines: 15 Message-ID: <41grv2$ces_002@dmbbs5.hol.gr> NNTP-Posting-Host: dmbbs5.hol.gr X-Newsreader: News Xpress Version 1.0 Beta #4 Dear all, I am a student of the Medical School Of Athens. I am working on a project regarding the role of platelets receptors. Libraries here in Greece have limited resources. I heard that I can find information on the Internet, but I am a little bit lost. Can you advise where and how I can get more information on the web or from other newsgroups. Thank you all in advance. Waiting desperately for your reply. George. From owner-biophysics@net.bio.net Wed Aug 21 23:00:00 1996 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!newsfeed.internetmci.com!news.sprintlink.net!news-stk-200.sprintlink.net!news.sprintlink.net!news-pen-14.sprintlink.net!news.sprintlink.net!news-pen-4.sprintlink.net!nntp.bnl.gov!usenet From: capel Newsgroups: bionet.biophysics Subject: Integration Software for X-ray Diffraction Date: Wed, 21 Aug 1996 06:48:47 -0400 Organization: biology dept., brookhaven national lab Lines: 19 Message-ID: <321AE98F.4584B88C@crim12b.nsls.bnl.gov> NNTP-Posting-Host: dispx12b.nsls.bnl.gov Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0b6 (X11; I; Linux 2.0.13 i586) I've posted a software suite for visualizing and integrating two-dimensional diffraction images. Currently the suite supports fuji, mar and multi-wire PSD detector formats, and has a wealth of different operational modes including: Circular, Sectorial, Norms along a central radial, Norms along an arbitrary vector, line/column extraction, Angular dependence of sector, Optimization of detector parameters, etc. Currently runs on IRIX and Linux, with ports to sun hp and vms currently in progress. It's documented and made available via anonymous ftp at URL: http://crim12b.nsls.bnl.gov/x12b_downloads.html M. Capel From owner-biophysics@net.bio.net Fri Aug 23 23:00:00 1996 Path: biosci!news.cc.sunysb.edu!usenet From: "Dr. James S. Trimmer" Newsgroups: bionet.biophysics Subject: Postdoc-Ion Channel Molecular Biology Date: Sat, 24 Aug 1996 11:38:34 -0400 Organization: State University of New York at Stony Brook Lines: 25 Message-ID: <321F21E9.6ADE@life.bio.sunysb.edu> NNTP-Posting-Host: trimmer.bio.sunysb.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Macintosh; I; PPC) Postdoctoral Position Molecular Neurobiology Position available immediately for biochemist or molecular biologist with previous experience using biochemical, molecular or genetic approaches to study protein-protein interactions. Ongoing projects involve 1) characterization of subunit interactions within mammalian K+ channel complexes (Neuron 16:843; J. Neurosci. 15:5360; 16:4846; JBC 271:7084), and 2) interactions of K+ channel complexes with proteins important in determining subcellular distribution (JCB in press). (Also see http://129.49.19.42/biochem/trimmer.html). Our laboratory is part of a strong and highly interactive interdepartmental molecular neurobiology group within the Institute for Cell and Developmental Biology at Stony Brook. Applicants must have a strong research background as evidenced by peer reviewed publications. Send curriculum vitae and names of three references to: Dr. James S. Trimmer Dept of Biochemistry and Cell Biology State University of New York Stony Brook, NY 11794-5215 The State University of New York at Stony Brook is an Equal Opportunity/Affirmative Action Employer From owner-biophysics@net.bio.net Sun Aug 25 23:00:00 1996 Path: biosci!iam.ubc.ca!sreich From: sreich@iam.ubc.ca (Sebastian Reich) Newsgroups: bionet.biophysics Subject: workshop announcement Date: 26 Aug 1996 12:52:30 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 86 Sender: daemon@net.bio.net Distribution: world Message-ID: <199608261952.MAA07465@net.bio.net> NNTP-Posting-Host: net.bio.net Following a suggestion ob Carol Gross, I would like to submit the following announcement to the bionet.biophysics newsgroup. Thank you. Sincerely, Sebastian Reich reich@zib-berlin.de Second International Symposium Algorithms for Macromolecular Modelling Konrad-Zuse-Zentrum Berlin May 21 -- 24, 1997 _______________________________________ an interdisciplinary symposium Following the success of the first symposium held in Lawrence in 1994, we like to announce the second (now international) symposium ``Algorithms for Macromolecular Modelling'' at the Konrad-Zuse-Zentrum Berlin on May 21 -- 24, 1997. The symposium will bring together scientists from various branches of (applied) mathematics, physics, chemistry, and biology who have been working on molecular dynamics and molecular modelling. The broad purposes of the symposium are (i) to provide an international forum for communicating state-of-the-art developments in molecular modelling algorithms and (ii) to improve the prospects for future international collaborations by emphasizing the involvement of younger scientists from both sides of the Atlantic. The emphasis of the symposium is on the numerical and algorithmic challenges to achieve dramatic gains in the performance and reliability of algorithms for the simulations of proteins, nucleic acids and other polymers. Topics appropriate to the symposium include: advanced time-stepping in molecular dynamics, electrostatics, quantum-classical dynamics, structure determination, and free energy and ensemble calculations. The workshop will take place Wednesday--Saturday in the new building of the Konrad-Zuse-Zentrum on the campus of the Freie Universit"at Berlin. The format will include invited presentations, contributed presentations (25 min.), and poster presentations. A partial list of speakers includes H.J.C. Berendsen (Groningen), J. Board (Duke), B.R. Brooks (NIH), H. Grubm"uller (Munich), W.F. van Gunsteren (ETH), J. Hermans (UNC), M. Holst (Caltech), M. Kuczera (KU), A. Mark (ETH), B. Leimkuhler (UK), S. Reich (ZIB), T. Schlick (NYU), K. Schulten (UIUC), Ch. Sch"utte (ZIB), R.D. Skeel (UIUC). The workshop is being organized by B.R. Brooks, W.F. van Gunsteren, J. Hermans, A. Mark, B. Leimkuhler, and R.D. Skeel with P. Deuflhard and S. Reich as the local organizing committee. Space is limited to about one hundred participants. Some funds might be available to assist with expenses (depending on our success in raising funds). For further information, registration and submission material see our www-page: http://elib.zib-berlin.de:88/MacroMM97/ Please address any further inquiries to Sebastian Reich Konrad-Zuse-Zentrum Berlin Heilbronner Str. 10 D-10711 Berlin - Germany e-mail: reich@zib-berlin.de From owner-biophysics@net.bio.net Sun Aug 25 23:00:00 1996 Path: biosci!agate!spool.mu.edu!sol.ctr.columbia.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in3.uu.net!server-b.cs.interbusiness.it!qualcuno.nettuno.it!sirio.cineca.it!gopher From: Albertino Bigiani Newsgroups: bionet.biophysics Subject: Donnan equilibrium and resting potential Date: Mon, 26 Aug 1996 22:30:06 +0200 Organization: University of Modena Lines: 13 Message-ID: <3222094E.2BF2@c220.unimo.it> NNTP-Posting-Host: slip-09.unimo.it Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; 68K) Hy biophysics! Here my question: is the normal resting potential of a cell due to a Donnan equilibrium or not? Many textbook states this and I wonder if this is true because the main ions (K, Na) involved are not at a thermodynamic equlibrium as a true Donnal equilibrium requires. So, what all about the Donnan equilibrium and why teaching it as an important mechanism for understanding the genesis of resting potential?. Am I missing some important facts here that explain why Donnan equlibrium is put in the chapter of resting potential? Thank you in advance. Ciao Albertino From owner-biophysics@net.bio.net Mon Aug 26 23:00:00 1996 Path: biosci!agate!newsxfer2.itd.umich.edu!howland.erols.net!cam-news-hub1.bbnplanet.com!uunet!in2.uu.net!dziuxsolim.rutgers.edu!usenet From: "Cinque S. Soto" Newsgroups: bionet.biophysics Subject: Re: Shareware & Freeware Physics Programs Date: Mon, 26 Aug 1996 21:05:38 -0400 Organization: Student at Rutgers University Lines: 20 Message-ID: <322249E2.50E6@eden.rutgers.edu> References: <01bb8f07$917927c0$1bb741ce@cen03887> Reply-To: 202, Orchard, Meadows, Drive, South NNTP-Posting-Host: mingus-b-asy-6.rutgers.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-poster: csoto@eden-dialup.rutgers.edu X-Mailer: Mozilla 3.0b6Gold (Win95; I) Tony Purkal wrote: > > I am a junior in high school. Could you find some shareware and freeware > physics program? If you find some please post the name of the program, and > a short description if possible, and their internet addresse. Any efforts > will be greatly appreciated! Tony Purkal: I believe you may wish to try to the PUB archives located at Indiana University. A really excellent program is Kinimage. This program allows you to view and rotate a three dimensional structure of a protein; it also allows you to program certain aspects to change certain coordinates...you have to see it to understand (I am relatively new to the program). If you do not have access to FTP, try this URL: pdb.pdb.bnl.gov/ Cinque Soto Rutgers University From owner-biophysics@net.bio.net Mon Aug 26 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!cam-news-hub1.bbnplanet.com!uunet!in2.uu.net!news.emi.com!pauling.wadsworth.org!tivol From: tivol@news.wadsworth.org (William Tivol) Newsgroups: bionet.biophysics Subject: Re: atmospheric humidity Date: 27 Aug 1996 17:00:22 GMT Organization: Wadsworth Center, NY Health Dept. Lines: 18 Message-ID: <4vv9j6$vp@pauling.wadsworth.org> References: <4vuoqm$eod@peng.ping.at> NNTP-Posting-Host: alcor.wadsworth.org X-Newsreader: TIN [version 1.2 PL2] Semper Andreas (asemper@ping.at) wrote: : Can anybody tell me how many liters of water there are in one m3 100 : degrees centigrade hot air at for instance 20 % relative atmospheric : humidity (e.g. a sauna). All the tables of atmospheric humidity I : found up to now end at 30 degrees centigrade. : If you have a table for also hotter temperatures please mail to : asemper@ping.at : Thanks Dear Andreas, My copy of the Handbook of Chemistry and Physics has a table of the vapor pressure of water at temps up to 374 C. 100% humidity is equal to the vapor pressure, and 20% would be 1/5 of that. In your example, there is a water vapor pressure of 152 mm Hg (0.2 at, since v.p.=1 at @ 100 C). Using PV=nRT, one can get the # of moles H2O/m3--I leave that as an exercise for you. Yours, Bill Tivol From owner-biophysics@net.bio.net Mon Aug 26 23:00:00 1996 Path: biosci!agate!newsxfer2.itd.umich.edu!uunet!in3.uu.net!EU.net!Austria.EU.net!news.ping.at!usenet From: asemper@ping.at (Semper Andreas) Newsgroups: bionet.biophysics Subject: atmospheric humidity Date: Tue, 27 Aug 1996 13:12:20 GMT Organization: ping - Personal InterNet Gate Lines: 8 Message-ID: <4vuoqm$eod@peng.ping.at> Reply-To: asemper@ping.at NNTP-Posting-Host: ts1-12.wienerneustadt.at.eu.net X-Newsreader: Forte Free Agent 1.0.82 Can anybody tell me how many liters of water there are in one m3 100 degrees centigrade hot air at for instance 20 % relative atmospheric humidity (e.g. a sauna). All the tables of atmospheric humidity I found up to now end at 30 degrees centigrade. If you have a table for also hotter temperatures please mail to asemper@ping.at Thanks From owner-biophysics@net.bio.net Mon Aug 26 23:00:00 1996 Path: biosci!agate!spool.mu.edu!uwm.edu!cs.utexas.edu!howland.erols.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!in3.uu.net!tank.news.pipex.net!pipex!oleane!jussieu.fr!univ-lyon1.fr!ura1195-5.univ-lyon1.fr!user From: fort@ura1195-6.univ-lyon1.fr (FORT Patrice) Newsgroups: bionet.biophysics Subject: BIO-LOGIC Date: 27 Aug 1996 09:04:05 GMT Organization: INSERM Lines: 7 Message-ID: NNTP-Posting-Host: ura1195-5.univ-lyon1.fr Pour ceux que ca interesse (forcement quelques uns dans ce newsgroup), BIO-LOGIC, la boite de Grenoble specialisee en materiels d'electrophy, a un serveur WEB. Si ca vous interesse, je peux vous transmettre l'adresse. De plus, il y a enfin un distributeur francais d'Axon Instruments. Si ca vous dit aussi.... Patrice From owner-biophysics@net.bio.net Tue Aug 27 23:00:00 1996 Path: biosci!FUGAZI.MICRO.UMN.EDU!gopher From: gopher@FUGAZI.MICRO.UMN.EDU (Gopher Client) Newsgroups: bionet.biophysics Subject: (none) Date: 28 Aug 1996 06:43:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: <9608281330.AA19753@fugazi.micro.umn.edu> NNTP-Posting-Host: net.bio.net Mail deleted because of lack of disk space. From owner-biophysics@net.bio.net Tue Aug 27 23:00:00 1996 Path: biosci!iam.ubc.ca!sreich From: sreich@iam.ubc.ca (Sebastian Reich) Newsgroups: bionet.biophysics Subject: workshop announcement Date: 28 Aug 1996 11:04:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 70 Sender: daemon@net.bio.net Distribution: world Message-ID: <199608281804.LAA22114@net.bio.net> NNTP-Posting-Host: net.bio.net Dear coordinators, I would like to poste the included workshop announcement in the bionet.biophysics newsgroup. Thank you. Sincerely, Sebastian Reich Second International Symposium Algorithms for Macromolecular Modelling Konrad-Zuse-Zentrum Berlin May 21 -- 24, 1997 _______________________________________ The symposium will bring together scientists from various branches of (applied) mathematics, physics, chemistry, and biology who have been working on molecular dynamics and molecular modelling. The emphasis of the symposium is on the numerical and algorithmic challenges to achieve significant gains in the performance and reliability of algorithms for the simulations of proteins, nucleic acids and other polymers. Topics appropriate to the symposium include: advanced time-stepping in molecular dynamics, electrostatics, quantum-classical dynamics, structure determination, and free energy and ensemble calculations. A partial list of speakers includes H.J.C. Berendsen (Groningen), J. Board (Duke), B.R. Brooks (NIH), R.B. Gerber ( Jerusalem), H. Grubm"uller (Munich), W.F. van Gunsteren (ETH), J. Hermans (UNC), M. Holst (Caltech), M. Kuczera (KU), A. Mark (ETH), B. Leimkuhler (UK), S. Reich (ZIB), T. Schlick (NYU), K. Schulten (UIUC), Ch. Sch"utte (ZIB), D. Shalloway (Cornell), R.D. Skeel (UIUC). The workshop is being organized by B.R. Brooks, W.F. van Gunsteren, J. Hermans, A. Mark, B. Leimkuhler, and R.D. Skeel with P. Deuflhard and S. Reich as the local organizing committee. Dealine for registration and submission is Nov. 15, 1996. The number of participants is restricted to about one hundred. Please address any further inquiries to Sebastian Reich Konrad-Zuse-Zentrum Berlin Heilbronner Str. 10 D-10711 Berlin - Germany http://elib.zib-berlin.de:88/MacroMM97/ From owner-biophysics@net.bio.net Tue Aug 27 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!paladin.american.edu!news.jhu.edu!news From: rlk Newsgroups: bionet.biophysics Subject: data fitting problems Date: Wed, 28 Aug 1996 14:37:34 -0400 Organization: Johns Hopkins University Lines: 30 Message-ID: <322491EE.167E@intrepid.chm.jhu.edu> NNTP-Posting-Host: 128.220.23.72 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (X11; I; IRIX 5.2 IP20) I've run into a problem trying to use the Grafit software package, and was wandering if anyone had any suggestions. I am studying an enzyme which exhibits a ping-pong bi-bi kinetic mechanism. However, the first substrate is a competitive inhibitor for the second substrate, and thus I am trying to fit my kinetic data to this eqn: v(i)= (Vmax*[A]*[B])/(Kma*[B]+Kmb*(1+[A]/Kia)*[A]+[A]*[B]) When I perform a global fit of this data I get an obviously incorrect answer independent of my initial estimates or weighting method (simple or robust.) Not only is this result incorrect just from visual inspection, it disagrees strongly with the values I achieve using more traditional data fitting methods (replots of slopes and intercepts.) Furthermore, if I rearrange the eqn somewhat, replacing the 1/Ki with just an 'X' which i manually reconvert to a Ki value, I get a markedly different set of results. This, I should think, should definately not happen if I got the eqn entered correctly. -- -ron ------------------------------------------------ 'If I can't dance, I don't want to be a part of your revolution' Emma Goldman From owner-biophysics@net.bio.net Wed Aug 28 23:00:00 1996 Newsgroups: bionet.biophysics Path: biosci!ihnp4.ucsd.edu!gondor!newsfeeder.sdsu.edu!news.sgi.com!news.msfc.nasa.gov!newsfeed.internetmci.com!in3.uu.net!hearst.acc.Virginia.EDU!murdoch!faraday.clas.Virginia.EDU!ana4a From: ana4a@faraday.clas.Virginia.EDU (Alex Artsyukhovich) Subject: FS: 97 Hi Power Optical Filter Set X-Nntp-Posting-Host: faraday.clas.virginia.edu Message-ID: Sender: usenet@murdoch.acc.Virginia.EDU Organization: uva Date: Wed, 28 Aug 1996 23:56:54 GMT Lines: 19 Greetings, I'm trying to sell a 97 high power optical filter set that I have. The set covers near UV(from 240 nm) all visible range and near IR(to 3 um). It contains cut-off, band pass and neutral density filters. For spectral curves and more information please refer to the webpage: http://faraday.clas.virginia.edu/~ana4a/Filters2.html The set is excellent for harmonics filtration, fluorescence spectroscopy, all sorts of thermal lens and thermal deflection spectroscopy. A combination of 2 and 3 filters out of 97 gives you practically unlimited freedom of experiment Hope to hear from you. Alex Artsyukhovich ph.:510/549-9049 e4-mail:ana4a@Virginia.EDU From owner-biophysics@net.bio.net Wed Aug 28 23:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.indigo.ie!usenet From: "Alan C Bruce" Newsgroups: bionet.biophysics Subject: Can you help me search for a "Pigment" Date: 29 Aug 1996 19:46:21 GMT Organization: Alan Corporation Lines: 11 Message-ID: <01bb95e2$50f06560$4e927dc2@indigo.ie> NNTP-Posting-Host: indigo14678.indigo.ie X-Newsreader: Microsoft Internet News 4.70.1155 In reseraching a particular project, I am am trying to source a substance/pigment (when viewed normally is translucent) which can be used on the skin (without any adverse effects) which when viewed through a relatively inexpensive filter (eg UV Sunglasses) would show up not translucent but as a bright colour. I would be most appreciative of any assistance you could give or even if you could point me in the right direction. acbruce@indigo.ie From owner-biophysics@net.bio.net Thu Aug 29 23:00:00 1996 Path: biosci!agate!howland.erols.net!spool.mu.edu!usenet.eel.ufl.edu!warwick!leicester!usenet From: "Dr E. Buxbaum" Newsgroups: bionet.biophysics Subject: Re: Donnan equilibrium and resting potential Date: 30 Aug 1996 08:45:40 GMT Organization: University of Leicester, UK (PCFS User) Lines: 7 Message-ID: <5069nk$3k4@falcon.le.ac.uk> References: <3222094E.2BF2@c220.unimo.it> NNTP-Posting-Host: pc47.msb.le.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 16bit) You have to distinguish two effects: The Donnan potential due to different mobility of ions and the potential created by active processes (transport ATPases). Under physiological conditions these are superimposed. To measure the Donnan potential, you'd have to block active transport (low temperature, ATP depletion, specific inhibitors like vanadate). From owner-biophysics@net.bio.net Fri Aug 30 23:00:00 1996 Path: biosci!agate!howland.erols.net!news3.cac.psu.edu!news.cse.psu.edu!news.ecn.bgu.edu!vixen.cso.uiuc.edu!newsfeed.internetmci.com!in2.uu.net!news.i-link.net!usenet From: Karl Kortemeyer Newsgroups: bionet.biophysics Subject: Symposium Date: 31 Aug 1996 07:03:13 GMT Organization: I-Link Inc Lines: 50 Message-ID: <508o3h$1d44@news.i-link.net> NNTP-Posting-Host: dallas-1-1.i-link.net Second Annual Advanced Water Sciences Symposium October 4 - 6, 1996, Dallas, Texas Sponsored by Institute for Advanced Water Sciences Research and the Seraph Foundation For more information or to register: phone (214) 682-9162 or see symposium web site at http://vitalenergy.com/water. This conference/symposium is hosted by the Institute for Advanced Water Sciences Research. IAWSR is a not for profit membership association dedicated to the promotion of advanced water sciences and the free exchange of ideas that contribute to the advancement of water science. IAWSR and the symposium provide a forum for members and contributing individuals to exchange ideas in the following water related subjects: Confirmed invited speakers: Joseph Bender: keynote speaker "Water: the Bigger Picture", and also Jim Beal, Ron Carson, Dr. Lee Cowden MD, Prof. Carlton Hazlewood, Dr. Stuart Hameroff, Dr. Tony Scott-Morley MD, Prof. Elizabeth Rauscher, Dr. William Rea MD, Glen Rein, Prof. James Roberts, Joseph Tyls. Hard Science: hydrodynamics, water purification technology, electrolysis, bond angle relationships and energy states, environmental issues, photo chemistry, ionics, ORP, chemistry, oxidative states in water. Interdisciplinary: vortex mechanics, magnetic resonance in water, liquid crystal states, advanced ozonation, colloidal chemistry, structured water, homeopathic effects in relationship to energized water, radio frequency effects on water, energization states in water, magnetic field effects on water dynamics and structure, energy systems utilizing water dynamics or structure. Exploratory: subtle energy effects in water, consciousness and water, effects of harmonic geometries on water, scalar wave effects on water, energization and transformation in the water molecule, memory in water states, biological effects of energized and structured water. Second Advanced Water Sciences Symposium P.O. Box 1295 Dallas, Texas 75355-1295 phone:(214) 682-9162 fax: (214) 827-6575 email: seraph@metronet.com From owner-biophysics@net.bio.net Fri Aug 30 23:00:00 1996 Path: biosci!agate!spool.mu.edu!uwm.edu!cs.utexas.edu!howland.erols.net!vixen.cso.uiuc.edu!cdc2.cdc.net!news.texas.net!news From: tibetcon@sedona.net (Val Eimers) Newsgroups: bionet.biophysics Subject: benefits of algae Date: Sat, 31 Aug 1996 20:51:58 GMT Organization: Sedona Internet Services, Inc. Lines: 26 Message-ID: <50ac8g$or9@news2.texas.net> Reply-To: tibetcon@sedona.net NNTP-Posting-Host: client55.sedona.net X-Newsreader: Forte Free Agent 1.0.82 If you would be interested in adding Super Blue Green Algae (TM) to supplement your diet, please contact me and I will supply you with some information. Sharing this information is important to me since I know that the algae can be beneficial. I especially think about kids and the benefits algae can offer for their growth. My son and I have been taking algae for about a year and I've noticed several differences in our health. Alleviation of my son's allergy-like symptoms is one of the changes. Super Blue Green Algae (TM) is a food containing over 60% protein, with all the essential amino acids our bodies need (easier to have a meatless diet); algae also contains almost all the minerals and vitamins we need. In addition, if you choose to, you may take advantage of the business opportunity Cell Tech offers. However, there is no pressure to do so. I'd like to hear from you, either e-mail me, (Val@Sedona.net). Or call me, Val Eimers, I'm an Independent Distributor with Cell Tech, (520) 284-4220, or write to: P.O. Box 20471, Sedona, AZ 86341. Approval # 113190