From owner-biophysics@net.bio.net Sat Nov 02 22:00:00 1996 Path: biosci!nntp.ucr.edu!rumors.ucr.edu!not-for-mail From: Student Newsgroups: bionet.biophysics Subject: Biophysics Conference in New Orleans Date: Sun, 03 Nov 1996 01:02:54 -0800 Organization: Student Net Lines: 3 Message-ID: <327C5FBE.319C@students.net> Reply-To: student@students.net NNTP-Posting-Host: lothlab5.ucr.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (WinNT; I) Hi. I was wondering if anyone can provide me more information on the Biophysics Conference in New Orleans for 1997. Just post a reply. Thanks From owner-biophysics@net.bio.net Sat Nov 02 22:00:00 1996 Path: biosci!agate!howland.erols.net!surfnet.nl!swidir.switch.ch!news.belwue.de!news.uni-kl.de!sun.rhrk.uni-kl.de!nenno From: nenno@rhrk.uni-kl.de (Mario Nenno [Bio.]) Newsgroups: bionet.biophysics Subject: Re: DNA fingerprinting Date: 3 Nov 1996 15:47:50 GMT Organization: Universitaet Kaiserslautern Lines: 28 Message-ID: <55ier6$h1o@sun.rhrk.uni-kl.de> References: <558au7$enm@newsbf02.news.aol.com> NNTP-Posting-Host: aix4.rhrk.uni-kl.de X-Newsreader: NN version 6.5.0 #7 (NOV) collins@UMBI.UMD.EDU ("John H. Collins, Ph.D.") writes: >Will someone kindly send me a primer on this topic? --------------------------------------------------------- Hallo John, Here are one review and three books as a possible primer: Epplen J. T., Buitkamp J., Bocker T., Epplen C. (1995) Indirect gene diagnoses for complex (multifactorial) diseases - A review. Gene 159: 49-55. Weising K, Nybom H, Wolff K, Meyer W. (1995). DNA Fingerprinting in Plants and Fungi. CRC Press, Boca Raton, U.S.A. ISBN 0-8493-8920-8 Pena SDJ, Chakraborty R., Epplen JT, Jeffreys AJ (Eds)(1993). DNA Fingerprinting: State of the Science. Birkh"auser Verlag, Basel, 466pp. ISBN 3-7643-2906-8 or ISBN 0-8176-2906-8 Burke T, Dolf G, Jeffreys AJ, Wolff R (Eds) (1991). DNA Fingerprinting: Approaches and Applications. Birkh"auser Verlag, Basel, 400pp. ISBN 3-7643-2562-3 or ISBN 0-8176-2562-3 From owner-biophysics@net.bio.net Sun Nov 03 22:00:00 1996 Path: biosci!rutgers!news.sgi.com!news.sprintlink.net!news-peer.sprintlink.net!news.sprintlink.net!news-hub.sprintlink.net!news.sprintlink.net!news-fw-22.sprintlink.net!moonbeam.aecom.yu.edu!usenet From: gottfrie@post.aecom.yu.edu (David S. Gottfried) Newsgroups: bionet.biophysics Subject: Re: Biophysics Conference in New Orleans Date: 4 Nov 1996 14:01:19 GMT Organization: Albert Einstein College of Medicine Lines: 14 Message-ID: <55ksvf$d95@moonbeam.aecom.yu.edu> References: <327C5FBE.319C@students.net> NNTP-Posting-Host: marcus.aecom.yu.edu X-Posted-From: InterNews 1.1@marcus.aecom.yu.edu X-Authenticated: gottfrie on POP host post.aecom.yu.edu In article <327C5FBE.319C@students.net> Student writes: > Hi. I was wondering if anyone can provide me more information on the > Biophysics Conference in New Orleans for 1997. Just post a reply. If you can, check out the Biophysical Society home page at: http://biosci.cbs.umn.edu/biophys/biophys.html They have all of the information there. ---------------------- David S. Gottfried AECOM - Biophysics gottfrie@aecom.yu.edu From owner-biophysics@net.bio.net Sun Nov 03 22:00:00 1996 Path: biosci!biosci!not-for-mail From: M J Geisow Newsgroups: bionet.general,bionet.biophysics,bionet.plants Subject: CALL FOR PAPERS: POPE'96 Date: 4 Nov 1996 11:40:45 -0800 Organization: BIODIGM Lines: 54 Sender: daemon@net.bio.net Distribution: world Message-ID: <3278251D.6B6@dial.pipex.com> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.general:23876 bionet.biophysics:2394 bionet.plants:13143 6th International conference: Perspectives on Protein Engineering John Innes Centre, Norwich, UK 29 June - 1 July 1997 FIRST ANNOUNCEMENT and CALL FOR PAPERS TOPICS Bioinformatics, Microbial genomes, Molecular Evolution and Design Plant Protein Engineering and biocatalysis SPEAKERS SO FAR Clare Fraser (The Institute for Genomic Research,USA) Microbial Genomes Mike Bevan (John Innes Institute) The Arabidopsis Genome Rik Wierenga (EMBL Heidelberg) Engineering and analysing protein loops Shuguang Zhang (MIT, Boston, USA) Design of self-assembling biomolecules Iain Campbell (Oxford UK) Modular protein evolution and design Jose Marcos (CSIC, Spain) Molecular diversity: Design of peptide agents against plant pathogens Udo Conrad (Gatersleben, Germany) Expression of antibodies in plants Paul Davies (Unilever, UK) Large scale antibody fragments expression in crop plants George Lomonosoff (John Innes Centre, UK) Vaccine procution in plants Chris Schofield (Oxford, UK) Dioxygenases: structure and action L Mario Amzel (Johns Hopkins, USA) Lipoxygenases Geoff Fincher (Adelaide, Australia) beta-glucanases Birte Svensson (Carlsberg Copenhagen, Denmark) amylases Richard Pickersgill (Reading, UK) Papaya proteinases John Raferty (Sheffield, UK) Fatty acid synthetases Didier Marion (Nantes, France) Lipid binding proteins Mike Lawrence (Parkeville, Australia) 7S/11S globulins Peter Shewry (Bristol, UK) Storage proteins POSTER SESSIONS & EVENING WORKSHOPS (to be announced) PUBLICATION On the World Wide Web and as a (ISBN) CD-ROM: http://www.biodigm.com/pope/cdrom.htm DETAILS AT THE CONFERENCE 'VIRTUAL MEETINGS OFFICE' Programme, venue, travel, on-line registration at: http://www.biodigm.com/pope/pope6.htm SECRETARIAT POPE6 c/o BIODIGM, 64, Langdale Grove, Bingham, NG13 8SS UK Fax: +44 1 949 876 156 E-mail: biodigm@dial.pipex.com PUBLICATION On the Internet and CD-ROM From owner-biophysics@net.bio.net Mon Nov 04 22:00:00 1996 Path: biosci!CS.Arizona.EDU!news.Arizona.EDU!hamblin.math.byu.edu!sol.ctr.columbia.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!demos!satisfy.kiae.su!news.e-burg.ru!newsserv From: "Yuri M. Linder" Newsgroups: bionet.biophysics Subject: Wanted storage of frozen bodies of ill people Date: 5 Nov 1996 19:07:20 +0500 Organization: Privater Person Lines: 8 Sender: newsserv@news.e-burg.ru Distribution: world Message-ID: Reply-To: yml@fabula.e-burg.ru NNTP-Posting-Host: news.e-burg.ru Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Keywords: storage, body, incurable X-Return-Path: fabula!fabula.e-burg.ru!yml@news.e-burg.ru Wanted address or phone organiztion or company that stores bodies of hopeless ill people in frozen state untill medicine be able to treat these deseases. Thanks, Yuri Linder yml@fabula.e-burg.ru Fax: (007 3432) 62-1771 From owner-biophysics@net.bio.net Mon Nov 04 22:00:00 1996 Path: biosci!LEX.LCCC.EDU!rcb1 From: rcb1@LEX.LCCC.EDU (Ron Blue) Newsgroups: bionet.biophysics Subject: Re: Wanted storage of frozen bodies of ill people Date: 5 Nov 1996 14:32:15 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Check the internet for ALCOR, Inc or TransTime, Inc. Currently NO one can be unfrozen. I believe if you raise the presure fast it will get the body beyond the range of the temperature that ice crystals form. Ron Blue On 5 Nov 1996, Yuri M. Linder wrote: > Wanted address or phone organiztion or company that > stores bodies of hopeless ill people in frozen state > untill medicine be able to treat these deseases. > > Thanks, Yuri Linder > > yml@fabula.e-burg.ru Fax: (007 3432) 62-1771 > > > From owner-biophysics@net.bio.net Sat Nov 09 22:00:00 1996 Newsgroups: bionet.general,bionet.neuroscience,bionet.biophysics Path: biosci!rutgers!uwm.edu!cs.utexas.edu!howland.erols.net!newsfeed.internetmci.com!in2.uu.net!hearst.acc.Virginia.EDU!murdoch!avery.med.Virginia.EDU!jjp2h From: jjp2h@avery.med.Virginia.EDU (Joseph J. Pancrazio) Subject: Scientist Positions Available X-Nntp-Posting-Host: avery.med.virginia.edu Message-ID: Sender: usenet@murdoch.acc.Virginia.EDU Organization: University of Virginia Date: Sun, 10 Nov 1996 13:18:42 GMT Lines: 39 Xref: biosci bionet.general:23958 bionet.neuroscience:16668 bionet.biophysics:2402 SAIC Job Posting The Biotechnology Research and Applications Division, a multidisciplinary group within the Life Science Operation at SAIC, is pursuing front- line research in areas of developmental neuro- physiology, neural prosthetics, biosensors and bio- compatibility. We are inviting applications from energetic and committed Ph.D.-level investigators in the following areas: Electrophysiology, Research Associates, two positions. Requirements: 1-3 years experience in patch-clamp re- cording of cells from primary neuronal culture and cell lines; knowledge of computer applications for data acqui- sition. Background in Physiology, Pharmacology or Biomedical Engineering preferred. Research Associates will be hired under three (3) year appointments with the opportunity to progress to senior scientist level. Successful candidates must be U.S. citizens or permanent residents. Interested candidates should forward a complete Curriculum Vitae with representative publications, along with salary requirements to: Science Applications International Corporation 1710 Goodridge Drive Department JHB64 McLean, Virginia 22102 NO E-MAIL REPLIES WILL BE CONSIDERED! ******************************************************** ** SAIC will offer interviews at the upcoming Society ** ***** for Neuroscience meeting in Washington, DC ******* ******************************************************** From owner-biophysics@net.bio.net Mon Nov 11 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!news3.cac.psu.edu!news.cse.psu.edu!news.cc.swarthmore.edu!netnews.upenn.edu!axe1.med.upenn.edu!axelsen From: axelsen@axe1.med.upenn.edu (Paul H Axelsen) Newsgroups: bionet.biophysics Subject: RESEARCH POSITION: thrombosis - biochemistry/biophysics Date: 12 Nov 1996 13:17:09 GMT Organization: University of Pennsylvania Lines: 29 Message-ID: <569tcl$hnb@netnews.upenn.edu> NNTP-Posting-Host: axe1.med.upenn.edu X-Newsreader: TIN [version 1.2 PL2-upenn1.1] We are seeking a person for biochemical/biophysical research on membrane damage, thrombogenesis, and atherogenesis at Penn. The position may be configurable as a Research Technician, Post-Doctoral Fellowship, Research Associate, or Assistant Professor, Research Track as appropriate for the background, experience, and goals of the applicant. The project involves infrared spectroscopy, fluorescence spectroscopy, and various forms of mass spectrometry. This is an excellent opportunity for someone seeking experience at the Chemistry/Biology interface on a problem of broad biomedical importance. For more information, please inquire via Email. -- --------------------- axe@pharm.med.upenn.edu -------------------------------- Paul H. Axelsen MD .... .... . . . . 215-898-9238 (ofc) Department of Pharmacology . . . .. . .. . 215-898-9766 (lab) University of Pennsylvania .... ... . . . . . . 215-573-2236 (fax) 3620 Hamilton Walk . . . .. . .. Philadelphia, PA 19104-6084 . .... . . . . ------------------------------------------------------------------------------ From owner-biophysics@net.bio.net Mon Nov 11 22:00:00 1996 Path: biosci!CC.USU.EDU!arsphys From: arsphys@CC.USU.EDU (Phil Harrison) Newsgroups: bionet.biophysics Subject: Spams have reached critical mass Date: 12 Nov 1996 08:31:53 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IBR569LM5YD43D3S@cc.usu.edu> NNTP-Posting-Host: net.bio.net I have enjoyed reading this list, but the unmoderated nature of the list has allowed so many spams/unwanted advertisement that it has become a problem. In other words, subscribing to the list has become for me more bother than it is worth. In still other words, the nuisance / benefit ratio has exceeded unity. Therefore, I shall be unsubscribing. (I also know that this message does not unsubscribe me, and I shall use the appropriate protocol to do that.) Best wishes to those who remain. Phil Harrison From owner-biophysics@net.bio.net Mon Nov 11 22:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!Newsmaster From: Jean-Philippe Demaret Newsgroups: bionet.biophysics Subject: (no subject) Date: 12 Nov 1996 15:33:53 GMT Organization: Universites Paris VI/Paris VII - France Lines: 18 Message-ID: <56a5d1$ij0@vishnu.jussieu.fr> NNTP-Posting-Host: lpbcsgi3.lpbc.jussieu.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1IS (X11; I; IRIX 5.3 IP22) X-URL: news:bionet.biophysics I am looking for a database (on any support : book, cd-rom, net server, etc...) which lists organic molecules along with their physico-chemical properties. More specifically, I am looking for molecules which exhibit major changes in their fluorescence spectra (increased yield or wavelength shift) in presence of double stranded DNA only. Can anyone help, please ? Dr Jean-Philippe Demaret L.P.B.C. Universite Pierre et Marie Curie place Jussieu 4 75252 Paris cedex 05 France fax : 00.33.1.44.27.75.60 email : jph@lpbc.jussieu.fr From owner-biophysics@net.bio.net Mon Nov 11 22:00:00 1996 Path: biosci!bloom-beacon.mit.edu!eru.mt.luth.se!www.nntp.primenet.com!nntp.primenet.com!dispatch.news.demon.net!demon!netcom.net.uk!nntpfeed.doc.ic.ac.uk!sunsite.doc.ic.ac.uk!lyra.csx.cam.ac.uk!daresbury!usenet From: blf@indigo7.dl.ac.uk (L.M.Murphy) Newsgroups: bionet.biology.n2-fixation,bionet.biophysics Subject: Re: Carlbiotech Ltd Date: 12 Nov 1996 13:51:46 GMT Organization: Daresbury Laboratory, Warrington, UK Lines: 20 Distribution: world Message-ID: References: <32721E28.2333@biosci.cbs.umn.edu> NNTP-Posting-Host: indigo7.dl.ac.uk In-reply-to: neil@BIOSCI.CBS.UMN.EDU's message of 26 Oct 1996 12:18:33 -0700 Xref: biosci bionet.biology.n2-fixation:566 bionet.biophysics:2404 Hi, I am trying to find the address/fax/phone number for the company Carlbiotech Ltd, Copenhagen, Denmark If anyone has this information I would be grateful if they could please forward it direct to me. Thanks in anticipation Lorrie Murphy L.M.Murphy@dl.ac.uk From owner-biophysics@net.bio.net Tue Nov 12 22:00:00 1996 Path: biosci!agate!howland.erols.net!www.nntp.primenet.com!nntp.primenet.com!usenet.eel.ufl.edu!warwick!lyra.csx.cam.ac.uk!daresbury!not-for-mail From: patricia@quimica.urv.es (Patricia Vaz) Newsgroups: bionet.biophysics Subject: chemx Date: 13 Nov 1996 10:34:18 -0000 Lines: 8 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <56c87a$5ek@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk does anybody know and use the chemx suite of programmes (including the quantum mechanical package)? I would be very grateful to get informations about it. cheers From owner-biophysics@net.bio.net Wed Nov 13 22:00:00 1996 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!howland.erols.net!surfnet.nl!swidir.switch.ch!serra.unipi.it!news.caspur.it!usenet From: Leopoldo Silvestroni Newsgroups: bionet.biophysics Subject: oxygen microelectrodes Date: Thu, 14 Nov 1996 18:57:16 -0800 Organization: Dip Fisipatologia Medica - Univ Roma 'La Sapienza' Lines: 15 Message-ID: <328BDC0C.6B57@CASPUR.IT> NNTP-Posting-Host: asynccgw3-11.caspur.it Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 3.01Gold (Win16; I) I need to measure intratissutal oxygen tension in rat organs in vivo. Does anyone know where to buy microelectrodes suitable for those measurements ? your help is greatly apreciated thank You all Leo -- Leopoldo Silvestroni Dipartimento di Fisiopatologia Medica Policlinico Umberto I, Universit di Roma "La Sapienza" Viale del Policlinico, 00161 - ROMA tel. +39-6-49970710; fax +39-6-4461450 e-mail L.SILVESTRONI@CASPUR.IT From owner-biophysics@net.bio.net Wed Nov 13 22:00:00 1996 Path: biosci!rutgers!news.sgi.com!howland.erols.net!news.sprintlink.net!news-peer.sprintlink.net!uunet!in1.uu.net!news.icix.net!news.gate.net!news From: DrHeasley@Chemistry.com (FAH) Newsgroups: bionet.general,bionet.cellbiol,bionet.biophysics Subject: Re: A Tale of Two Nuclei Date: Thu, 14 Nov 1996 16:33:49 GMT Organization: CyberGate, Inc. Lines: 24 Message-ID: <328b4926.17481812@news.gate.net> References: <1996Nov13.225604@carleton.edu> NNTP-Posting-Host: dfbfl5-7.gate.net X-Newsreader: Forte Agent .99e/32.227 Xref: biosci bionet.general:24037 bionet.cellbiol:5951 bionet.biophysics:2411 On 13 Nov 96 22:56:04 CST, noskovik@carleton.edu ( ) wrote: |Hello. I am an undergrad student with a question for a knowledgable |biologist. |Is it possible (even if only in theory) to fuse two nuclei? Can two sperm |nuclei or two egg nuclei be fused by any method or their genetic material |combined into one nucleus? |If you can help me out, please mail me at Noskovik@carleton.edu. Thanks in |advance. |Kyle Noskoviak Consider this as a side comment - according to Christian doctrine, this has occurred at least once. However, the fusion of two egg nuclei would, of course, produce an XX offspring, and as we know in this case, the genetic makeup was XY (unless there's something we haven't been told). ;-) Frank Heasley, Ph.D. Principal FSG Online - Jobs and Resumes in Biotechnology, Science and Medicine http://www.chemistry.com From owner-biophysics@net.bio.net Wed Nov 13 22:00:00 1996 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!news.tamu.edu!news.utdallas.edu!nrchh45.rich.nt.com!bcarh189.bnr.ca!nott!nrcnet0.nrc.ca!BRI.NRC.CA!Feng.Ni From: Feng.Ni@BRI.NRC.CA (Feng Ni) Newsgroups: bionet.biophysics Subject: RESEARCH POSITIONS IN PROTEIN STRUCTURE AND DESIGN Date: 14 Nov 1996 21:49:58 GMT Organization: Institut de recherche en biotechnologie, Montral Lines: 46 Distribution: world Message-ID: <56g466$j6s@nrcnet0.nrc.ca> NNTP-Posting-Host: indy.bri.nrc.ca RESEARCH POSITIONS IN PROTEIN STRUCTURE AND DESIGN The Biotechnology Research Institute (BRI) has openings for self-motivated researchers to study the structure-and-function relationships of proteins and to design novel proteins or peptides as potential pharmaceutical agents. The research will be focusing on the following areas: (1) proprotein convertases (PC's), calcium-sensing receptors (CaSRs), and CDC42-target complexes. Candi- dates should have a recent Ph.D. (within the last 5 years) in chemistry, bio- chemistry or a related field and have demonstrated abilities in carrying out creative research. Experiences include protein expression, purification and characterization, and the analysis of the structures of proteins by use of NMR or other biophysical techniques. Preferences will be given to candidates with extensive knowledge in the conformational stabilities and folding of proteins and peptides. Experience in the determination of the 3D structures of proteins by NMR is an important asset. Successful applicants will start in the postdoctoral level, or depending on qualifications, can be recruited to more senior positions offerred by the National Research Council of Canada. The Research Council offers competitive salaries and other compensation packages commensurate upon your experience and appointment levels. Researchers have access to state-of-the-art facilities for protein biochemistry. Structural analysis is carried out with two (500 and 800 MHz) NMR spectrometers along with required computing facilities. BRI offers an intellectually stimulating environment for multi-disciplinary research with expertise in molecular biology, protein/peptide chemistry, and structural biology. The Biotechnology Research Institute is a branch of the National Research Council of Canada, interfacing academia and industry. Your challenge will be to succeed in high-impact scientific research while generating applications transferable to pharmaceutical industry. Qualified individuals are invited to submit your resume, along with the names and addresses of three references, to: Feng Ni, Ph.D. Biotechnology Research Institute 6100 Royalmount Avenue Montreal, Quebec, H4P 2R2, Canada phone: (514)-496-6729; fax: (514)-496-5143 e_mail: fengni@bri.nrc.ca The positions will be available after January 1, 1997. Applications will be accepted until the positions are filled. We thank all applicants IN ADVANCE for their interests, however, only those being considered will be contacted. From owner-biophysics@net.bio.net Wed Nov 13 22:00:00 1996 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!news.tamu.edu!news.utdallas.edu!nrchh45.rich.nt.com!bcarh189.bnr.ca!nott!nrcnet0.nrc.ca!BRI.NRC.CA!Feng.Ni From: Feng.Ni@BRI.NRC.CA (Feng Ni) Newsgroups: bionet.biophysics Subject: RESEARCH POSITIONS IN PROTEIN STRUCTURE AND DESIGN Date: 14 Nov 1996 21:27:36 GMT Organization: Institut de recherche en biotechnologie, Montral Lines: 44 Distribution: world Message-ID: <56g2s8$i8l@nrcnet0.nrc.ca> NNTP-Posting-Host: indy.bri.nrc.ca RESEARCH POSITIONS IN PROTEIN STRUCTURE AND DESIGN The Biotechnology Research Institute (BRI) has openings for self-motivated researchers to study the structure-and-function relationships of proteins and to design novel proteins or peptides as potential pharmaceutical agents. The research will be focusing on the following areas: (1) proprotein convertases (PC's), calcium-sensing receptors (CaSRs), and CDC42-target complexes. Candi- dates should have a recent Ph.D. (within the last 5 years) in chemistry, bio- chemistry or a related field and have demonstrated abilities in carrying out creative research. Experiences include protein expression, purification and characterization, and the analysis of the structures of proteins by use of NMR or other biophysical techniques. Preferences will be given to candidates with extensive knowledge in the conformational stabilities and folding of proteins and peptides. Experience in the determination of the 3D structures of proteins by NMR is an important asset. Successful applicants will start in the postdoctoral level, or depending on qualifications, can be recruited to more senior positions offerred by the National Research Council of Canada. The Research Council offers competitive salaries and other compensation packages commensurate upon your experience and appointment levels. Researchers have access to state-of-the-art facilities for protein biochemistry. Structural analysis is carried out with two (500 and 800 MHz) NMR spectrometers along with required computing facilities. BRI offers an intellectually stimulating environment for multi-disciplinary research with expertise in molecular biology, protein/peptide chemistry, and structural biology. The Biotechnology Research Institute is a branch of the National Research Council of Canada, interfacing academia and industry. Your challenge will be to succeed in high-impact scientific research while generating applications transferable to pharmaceutical industry. Qualified individuals are invited to submit your resume, along with the names and addresses of three references, to: Feng Ni, Ph.D. Biotechnology Research Institute 6100 Royalmount Avenue Montreal, Quebec, H4P 2R2, Canada phone: (514)-496-6729; fax: (514)-496-5143 e_mail: fengni@bri.nrc.ca The positions will be available after January 1, 1997. Applications will be accepted until the positions are filled. We thank all applicants IN ADVANCE for their interests, however, only those being considered will be contacted. From owner-biophysics@net.bio.net Wed Nov 13 22:00:00 1996 Path: biosci!rutgers!news.sgi.com!esiee.fr!jussieu.fr!Newsmaster From: Jean Philippe Demaret Newsgroups: bionet.biophysics Subject: hoechst 33258 and 33342 Date: Thu, 14 Nov 1996 13:20:30 +0100 Organization: Universites Paris VI/Paris VII - France Lines: 9 Message-ID: <328B0E8E.2781E494@lpbc.jussieu.fr> NNTP-Posting-Host: lpbcsun.lpbc.jussieu.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (X11; I; SunOS 4.1.4 sun4m) Does anyone have informations about the fluorescence of Hoechst 33258 and 33342 in presence of single-stranded DNA ? And in presence of proteins ? Thanks. Dr Jean-Philippe Demaret L.P.B.C. tel : 00.33.1.44.27.27.50 Universite Pierre et Marie Curie fax : 00.33.1.44.27.75.60 place Jussieu 4 - case 138 email : jph@lpbc.jussieu.fr F-75252 Paris cedex 05, France From owner-biophysics@net.bio.net Wed Nov 13 22:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!online.no!Oslo2.Norway.EU.net!Norway.EU.net!nntp.uio.no!sn.no!www.nntp.primenet.com!nntp.primenet.com!mr.net!news.mr.net!carleton.edu!carleton.edu!noskovik From: noskovik@carleton.edu ( ) Newsgroups: bionet.general,bionet.cellbiol,bionet.biophysics Subject: A Tale of Two Nuclei Date: 13 Nov 96 22:56:04 CST Organization: Carleton College -- Northfield, MN Lines: 8 Message-ID: <1996Nov13.225604@carleton.edu> NNTP-Posting-Host: veblen.acns.carleton.edu Xref: biosci bionet.general:24026 bionet.cellbiol:5948 bionet.biophysics:2409 Hello. I am an undergrad student with a question for a knowledgable biologist. Is it possible (even if only in theory) to fuse two nuclei? Can two sperm nuclei or two egg nuclei be fused by any method or their genetic material combined into one nucleus? If you can help me out, please mail me at Noskovik@carleton.edu. Thanks in advance. Kyle Noskoviak From owner-biophysics@net.bio.net Thu Nov 14 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!www.nntp.primenet.com!nntp.primenet.com!uwm.edu!news.he.net!news.good.net!news.good.net!news.goodnet.com!news From: "B Willert" Newsgroups: bionet.biophysics Subject: T2 bacteriophage Date: 14 Nov 1996 06:12:28 GMT Organization: GoodNet Lines: 5 Message-ID: <01bbd24e$bfc59660$58ba62cf@default> NNTP-Posting-Host: tuc-ts1-23.goodnet.com X-Newsreader: Microsoft Internet News 4.70.1155 I am looking for any recent publications concerning the organization of DNA within the T2 or T4 bacteriophages. Any help would be very appreciated. Thank you very much, Bryan Willert From owner-biophysics@net.bio.net Thu Nov 14 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: thomas@cicada.berkeley.edu (Thomas Z. Lauritzen) Newsgroups: bionet.biophysics Subject: photorecepter cells Date: 15 Nov 1996 08:04:30 -0000 Lines: 11 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <56h86e$or5@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk Photoreceptor cells in the eye are dependent on interactions with other neurons in the eye. But how can you experimentally prove this? I was thinking about isolating photoreceptorcells and see that they die, but what proves that it is the missing interaction and not other things that kills them? Are there better ways, and does anybody know where I can find information or references about experiments? Thanks Thomas Lauritzen thomas@cicada.berkeley.edu From owner-biophysics@net.bio.net Thu Nov 14 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!vixen.cso.uiuc.edu!uwm.edu!msunews!news From: dassu@pilot.msu.edu (Sudipto Das) Newsgroups: bionet.biophysics Subject: test Date: 15 Nov 1996 15:57:49 GMT Organization: MSU Microbiology Lines: 2 Message-ID: <56i3tt$qe0@msunews.cl.msu.edu> NNTP-Posting-Host: 35.8.243.49 Mime-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII X-Newsreader: WinVN 0.99.7 test From owner-biophysics@net.bio.net Thu Nov 14 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.sgi.com!www.nntp.primenet.com!nntp.primenet.com!howland.erols.net!news.starnet.net!newsreader.wustl.edu!mdonken From: mdonken@artsci.wustl.edu (Michael Dorsett Onken) Newsgroups: bionet.general,bionet.cellbiol,bionet.biophysics Subject: Re: A Tale of Two Nuclei Followup-To: bionet.general,bionet.cellbiol,bionet.biophysics Date: 15 Nov 1996 17:41:14 GMT Organization: Washington University in St. Louis, MO USA Lines: 9 Message-ID: <56i9vq$55q@newsreader.wustl.edu> References: <1996Nov13.225604@carleton.edu> NNTP-Posting-Host: artsci.wustl.edu X-Newsreader: TIN [version 1.2 PL2] Xref: biosci bionet.general:24055 bionet.cellbiol:5962 bionet.biophysics:2419 noskovik@carleton.edu wrote: : Hello. I am an undergrad student with a question for a knowledgable : biologist. : Is it possible (even if only in theory) to fuse two nuclei? Can two sperm : nuclei or two egg nuclei be fused by any method or their genetic material : combined into one nucleus? : If you can help me out, please mail me at Noskovik@carleton.edu. Thanks in : advance. : Kyle Noskoviak From owner-biophysics@net.bio.net Sat Nov 16 22:00:00 1996 Path: biosci!MUWAYF.UNIMELB.EDU.AU!Frances_Separovic From: Frances_Separovic@MUWAYF.UNIMELB.EDU.AU (Frances Separovic) Newsgroups: bionet.biophysics Subject: PhD Scholarships Date: 16 Nov 1996 18:02:44 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 36 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Post-Graduate Research Scholarships: Biophysical Chemistry Ion Mobility in Polymer Gels Structure of Modified Ion Channels Positions are available for PhD and MSc research in the field of nuclear magnetic resonance (NMR) applications in Biophysical Chemistry. Projects include: (i) the use of NMR techniques to determine ion binding sites and ion mobility in polyethyleneglycol (PEG) and polyethyleneoxide (PEO) lipid conjugates; (ii) determination of the structure of modified ion channels, using NMR to study the mechanism of ion channel gating. These projects will involve collaboration with a multidisciplinary team from the Cooperative Research Centre for Molecular Engineering and Technology working on the development of biologically based sensors. Each project will involve interaction with University, CSIRO and industry based researchers. Research Scholarships: Candidates should have a Bachelors degree with H1 or H2A Class Honours. A background in physical chemistry, biophysics or biochemistry would be highly desirable. Supplementation of $5,000 pa for successful Australian Postgraduate Awardees will be considered by award of a Pacific Dunlop Scholarship for up to 3 years. Enquiries and applications (including a resume, academic record and names and addresses of two referees) should be directed to: Assoc. Professor Frances Separovic School of Chemistry University of Melbourne Parkville VIC 3000 Australia ph: 03 9344 6464 fax: 03 9347 5180 email: f.separovic@chemistry.unimelb.edu.au From owner-biophysics@net.bio.net Sat Nov 16 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.biophysics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Nov 1996 02:00:41 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611171000.CAA09412@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-biophysics@net.bio.net Sun Nov 17 22:00:00 1996 Path: biosci!agate!howland.erols.net!peerfeed.internetmci.com!newsfeed.internetmci.com!nol.net!not-for-mail From: Mark Peters Newsgroups: bionet.biophysics Subject: --> Cancer, Stealth Microbe Date: Sun, 17 Nov 1996 20:09:52 -0800 Organization: GENIUS QUEST Lines: 313 Message-ID: <328FE18F.7B6B@nol.net> Reply-To: gquest@nol.net NNTP-Posting-Host: ip38-84.nol.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Win16; U) STEALTH MICROBE CAUSES CANER There are two basic forms of carcinogens (cancer causers): EPIGENETIC - carcinogens that do not directly damage DNA but cause alterations such as hormonal derangements, immunosuppression or chronic tissue injury that in turn predisposes to cancer. GENOTOXIC - Carcinogens that react directly with DNA or with macromolucles that then react with DNA. The Stealth Microbe damages tissue by its presence, and alters hormone balance, and causes immunosuppression . The Stealth Microbe can cause the formation of the carcinogen NMBA by cell-mediated catalysis. Also he Stealth Microbe secretes organic acids during carbohydrate metabolism that can result in spontaneous nitrosation of the carcinogen NMBA.. The Stealth Microbe is EPIGENETICALLY AND GENOTOXICALLY CARCINOGENIC. The rest of this document will be dedicated to medical research that supports the statement that the stealth microbe causes Cancer. 1980 Studies of a population with a high incidence of esophageal carcinoma showed that Stealth Microbe invasion was common in the esophageal epithelium of patients with either premalignant changes or early esophageal carcinoma. Nitrate and Nitrite were present in high concentrations in the water and staple grains. This study established the ability of the Stealth Microbe to augment the nitrosative formation of the esophagus - specific carcinogen benzylmethylnitrosamine (NBMA; N-nitroso-N- methylbenzylamine). Results showed that when there were more microbes there was also more NBMA. Spontaneous nitrosation of BMA was enhanced under the acidic condidtions resulting from the acids secreted by the Stealth Microbe during Carbohydrate Metabolism. Thus, the Stealth Microbe invasion into the epithelium could cause local formation of NBMA by both cell- mediated catalysis and extracellular decreases in pH. 1987 From twelve cases of precancerous lesions, 21 strains of the Stealth Microbe were isolated belonging to 15 different biotypes. Stealth Microbes obtained via biopsy were accessed for their ability to catalyze N- nitrosobenzylmethylamine (NBMA) N-benylmethylamine and nitrite. Strains with the higher niltrosation potential were generally isolated from lesions with the more advanced precancerous changes. This evidence supports the hypothesis that the Stealth Microbe plays a causal role in the development of cancer, by means of endogenous nitrosamine production. The microbe is able to secrete organic acids during carbohydrate metabolism which decreases the local pH and this results in a considerable increase in the microbes production of the carcinogen NBMA. 1994 "The study was aimed at determination of possible connection between co-existing S.M. infection in patients with lungcarcinoma with course of neoplastic disease and ability to achieveremission by application of different methods of treatment. Thestudy involved 25 patients with histologically confirmed lung carcinoma. Their sputum and bronchial rinsings were tested. The tests were performed at the moment of diagnosisof the diseases, during and after treatment. In all patients so occurrence and type of S.M. infections was investigated .Dynamics of S.M. infection was measured by a scale from minimal to very severe. In about 1/3 of patients at the moment of diagnosisof the disease, severe or very severe infection was seen which was dependent of stage of the disease advancement. These preliminary studies seem to indicate that presence of severe or very severe S.M infection is not favorable prognostically and frequently correlates with a progress of neoplastic changes and worse responseto treatment." 1994 Patients with malignancies are at high risk to develop systemic non bacterial infections. Stealth Microbe accounts for approximately 80% of these isolates. The vast majority of these infections are of endogenous origin." HOST DEFENSES AND HOW Stealth Microbe DEFEATS THEM The skin and mucous membranes are primary defenses Stealth Microbe attacks and eats the skin The mucous membranes are primary defense Proteinase attacks secretory IgA of mucous membranes Gliotoxin attacks mucociliary action Macrophages protect the respiratory tract Gliotoxin inhibits macrophage phagocytosis Cell mediated immunity protects us from Stealth Microbe Antigen processing Macrophage activation (3a) Inhibited lymphokine production (3b) Inhibited activation of T lymphocytes Myeloperoxidase enzyme protects against mycoses Mannan inhibits myeloperoxidase release Phagocytic cells are the mainstay of cellular immunity Stealth Microbe grows from phagocytic cells Antiphagocytic compound Humoral immunity: opsonization helps protect us Proteinase reduces opsonizing activity Humoral immunity: complement C3 role in host defense Proteinase degradation of complement C3 Nonimmune serum factors: transferins bind iron Stealth Microbe siderophores sequester iron Lactobacillus produces potent S.M. growth inhibitor MORE ON Stealth Microbe PROTEINASE Cleavage of immunoglobulins including IgA FC Portion of immunoglobulin g degraded REDUCED BACTERICIDAL ACTIVITY AGAINST ESCHERICHIA COLI Alpha 2 macroglobulin & alpha 1 proteinase MORE ON MANNANS AND POLYSACCHARIDES Multiple inhibition of proliferative response Mannan inhibits lymphoproliferation Stealth Microbe specific inhibition proliferative response Glycoprotein & mannan suppress lymphoproliferation Mannan increases hydrogen peroxide production Polysaccharide antigens inhibit lymphocyte proliferation Polysaccharide depression of cellular function MORE ON GLIOTOXIN Gliotoxin present in vaginal samples Cultured cell growth blocked Gliotoxin reduces B lymphocytes count Irradiation increased gliotoxin toxicity Apoptosis DNA fragmentation in macrophages - apoptosis Apoptosis of mouse L929 fibroblast cells Apoptosis in concanavalin a-stimulated t blasts Apoptosis of T blasts and macrophages 80-95% inhibition of protein synthesis Lymphoid organ apoptosis Cytoskeletal structure damage & adhesion loss Kills non-hematopoietic cells TISSUE DAMAGE Extensive tissue damage Cytoskeletal microfilament/detachment/vacuolation Modification of microfilaments or plasma membranes Induction of microvilli loss Reorganization of cortical cytoplasm Altered bile acid uptake Afflux Modified hormone responsiveness (Stealth Microbe DIABETES will have much on hormones) "Incubation of Stealth Microbe cells with human luteinizing hormone (hLH), human chorionic gonadotrophin(hCG) Or glucagon produced a significant rise in cAMP total levels." Mice treated with cortisone were susceptible to intratracheal challenge with 10-100 x less Stealth Microbe than were untreated mice. "Stealth Microbe also possesses enzymes that can reduce steroids at the 3- ,17-and 20-oxo-groups. Altered morphology and plasma membrane function Tumor-like lesions on major organs DNA CHANGES Drastic DNA changes & precancerous tissue damage Raised inositol triphosphate & DNA fragmentation 30 ng/ml prevents T & B cell DNA synthesis DNA fragmentation of T and B cells Genome severely damaged Alloreactive cytotoxic T cells DAMAGE BELOW THE GENERALIZED TOXICITY THRESHOLD Significant DNA damage in thymocytes at um Glucose metabolism selectively affected Macromolecular synthesis selectively affected Basal rate of H2O2 production inhibited Macrophage activity abrogated LYMPHOPROLIFERATION T-cell proliferation inhibition Mitogen or interleukin induced b & t proliferation Leukocytes as stimulator cells disabled Depletion of murine epidermal Langerhans Major histocompatibility complex (nm) concentration ultrastructural damage to lc Immune response and contact sensitivity Increased suppressor cell activity of B-lymphocytes Phytohemagglutinin-induced mitogen response suppressed Anti-sheep erythrocyte antibody response suppressed Anti-thy 1 antibody and complement Not plastic-adherent or mac-1 antigen-positive Cytotoxic T cells production failure MACROPHAGES Macrophage phagocytosis irreversibly inhibited Microvilli loss and chromatin condensation In vivo macrophage & T cell dependent mechanisms Stealth Microbe TOXINS High molecular weight toxins Glycoprotein toxins SMtoxin Low molecular-weight toxins Stealth Microbe toxin investigation results Pathogenic enzymes Proteinase Phospholipases Lysophospholipase-transacylase WHAT Stealth Microbe IS Stealth Microbe - the most pervasive S.M. pathogen A facultative pathogen - masquerading marauder Pathogenic trickery Pathogenesis and phenotypic switching Differential expression of virulence factors Rapid alteration of phenotype 9 distinct colony phenotypes Colony morphology Constraints on the ___ __ _____ transition Cellular phenotype Patterns of gene expression Resistance to anti-S. M. agents Antigenicity Stealth Microbe has amphotericin b resistance Pathologic states are extremely varied Invasive potential increases with quantity MORE ON WHAT Stealth Microbe DOES Gut colony may impair cell mediated immunity Stealth Microbe is a cause of immunodeficiency Increased susceptibility to bacterial infection Suppressed DTH and bacterial sepsis Elevated Stealth Microbe antigen titers & bacterial sepsis World wide plague - often refractory Stealth Microbe infections are increasing in incidence How it isn't detected Molecular mimicry Antigenic variability Topographical variation dependent on carbon source Diagnostic skin test failure How it is diagnosed Successful post autopsy diagnosis Antemortem diagnosis is difficult Clinician's uncertainty Difficulty in differentiation - colony or invasion Invasive biopsy required Therapy by suspicion From owner-biophysics@net.bio.net Sun Nov 17 22:00:00 1996 Path: biosci!BIOSCI.MBP.MISSOURI.EDU!baskin From: baskin@BIOSCI.MBP.MISSOURI.EDU (Tobias Baskin) Newsgroups: bionet.biophysics Subject: "stir bar" equation Date: 18 Nov 1996 10:54:20 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Greetings, Does anyone know off hand, or know a reference where I could look up, an equation that relates the angular velocity of a rod around its midpoint (ie, a "stir bar") and the medium viscosity to force? I am particularly interested in the low Reynolds number solution where the mass of the "stir bar" is really small and can be neglected. Thanks for any leads. Tobias Baskin _ ____ _ __ ____ Tobias I. Baskin / \ / / \ / \ \ University of Missouri / | / / \ \ \ Biological Sciences /__ / /__ /___\ \ \__ 109 Tucker Hall / / / \ \ \ Columbia, MO 65211 USA / / / \ \ \ voice: 573-882-0173 / /____ / \ \__ / \____ fax: 573-882-0123 From owner-biophysics@net.bio.net Sun Nov 17 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!cs.utexas.edu!howland.erols.net!feed1.news.erols.com!uunet!in2.uu.net!news.atl.bellsouth.net!newsrelay.iastate.edu!news.iastate.edu!news From: S1.FAH@ISUMVS.IASTATE.EDU Newsgroups: bionet.biophysics Subject: NICE, EDUCATED MEN IN 515 AREA CODE Date: Mon, 18 Nov 96 08:32:43 CST Organization: Iowa State University, Ames, Iowa Lines: 95 Message-ID: <56ps2g$8e8@news.iastate.edu> NNTP-Posting-Host: isumvs.iastate.edu I am a single white female in my early 30's, on the short side but rather buxom, half Italian and half English, generally considered attractive, actually more "cute." I am a grad student (Ph.D.) in the social sciences and counseling. Interests include social issues, especially those of an international scope, history, and humorous movies. I am well-read, highly intellectual, quite international, somewhat traveled, a very good listener, highly empathetic, nonmaterialistic, devoted, warm, caring, very passionate as well as compassionate. I tend to laugh a lot and consider it as necessary as sleeping. I am not the sort of person that needs to be entertained, rather finds the company I am with more important than the activity shared together. I like stimulating conversation over the beverage of choice or long walks. I am also a nonsmoker, and drink very little. I am also very pragmatic and realistic, but with a streak of idealism. I am quite liberal, and like nontraditional gender roles (i.e.- 1950's Leave It to Beaver - yuck!). I am also very self-confident, but very humble at the same time. I have excellent communication skills, both verbally and nonverbally, and am very good at catching small nonverbal cues that others typically miss. I am terribly honest but tactful, almost to a fault. I am also nonreligious, but do consider religion important for societies in general, and for those who consider it important for themselves. I am very culturally relativistic, and dislike ethnocentrism (belief that one's own group or culture is superior to all others) in all forms. I also highly dislike prejudice and discrimination in all forms. I consider intimate relationships to be more important than anything including money or social and professional prestige. As a result, I have devoted a considerable amount of time to studying relationship dynamics, and what it takes to make a relationship work. I have been told I am high maintenance, but it is only because I am more than willing to do the work necessary to make a worthwhile relationship work, and expect the same from my partner. I am not a great cook, but love all sorts of ethnic foods. I love languages and can speak a smattering of a few, mostly Asian languages. To be honest, I am looking for a serious relationship that can lead to something permanent. I am seeking a highly educated, basically liberal man, with similar personality traits between the ages of 29 and 36 (give or take). He must love laughing, long walks, stimulating conversation (nothing is sacred, I AM a social scientist!), be a GOOD listener, the kind of man people turn to when they feel down or just want comfortable company is my type for sure! Frankly, I like "nice" men very much. I am not seeking someone who is flashy or vain, but rather has a quiet strength and self-confidence. I LIKE sensitive men, and those who have the sense of security to show their honest emotions in front of a woman (I regard such a display as a gift), and those who are secure enough not to be threatened by a strong, confident, assertive woman. I prefer someone who drinks little, and is absolutely a nonsmoker. I also prefer someone with good relationship and emotional management skills, as experience as taught me that possessing these skills (which I do) requires that they are best maintained by being with others of basically the same skill level, or at least those interested in developing these skills. I am serious about liking nice men (including those who consider themselves "nerds" as long as they possess relationship and emotional management skills). I consider the label of "nice" to refer to men who are kind, gentle, generous both emotionally and otherwise, who feel comfortable with themselves and others, and who are beyond the need to perform to prove their manhood to others, which leaves them free to concentrate on the needs of others in an empathetic manner, without feeling they are a pushover. I am especially attracted to those who know themselves, what they want, and what they are about. Grad students are preferred only because when you are a grad student it is nice to have someone to understands the pressure to discuss the pain and pleasure of the process with. ALL races and nationalities are welcome, but you must be internationally inclined. This means that you are very interested in things from an international perspective, and interested in non-American people, and somewhat comfortable with those from other cultures. If you are international and wish to reply, please do so only if you are planning to stay in the states permanently. And only those in Iowa or central Iowa please reply and only those who are planning to stay local for at least a year and have the time and interest towards working on a long term relationship. Those only interested in sex or something short-term need not reply. Anything less would be a waste of both of our time. I encourage grad students (especially Ph.D. students, as I am one) and those in academia, such as professors to reply. I intend to be a professor and family counselor when I am done, and find the intellectual and academic type particularly attractive. If you are an instructor (T.A.) or a professor and you wish to reply and are concerned about privacy, rest assured I will communicate with you with the utmost discretion. Thank you for reading this very long ad :( and I long forward to hearing from interested gentlemen. All letters will be responded to, but only those willing to write in detail about themselves will be seriously considered. Respectfully, F From owner-biophysics@net.bio.net Sun Nov 17 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!howland.erols.net!newsfeed.internetmci.com!newsfeed1.aimnet.com!news.sprintlink.net!news-dc-10.sprintlink.net!nntp.bnl.gov!usenet From: "m. capel" Newsgroups: bionet.biophysics Subject: Crystallography and Small Angle Diffraction at the NSLS Date: Sat, 16 Nov 1996 15:17:22 -0500 Organization: biology dept., brookhaven nat'l lab Lines: 15 Message-ID: <328E2152.41C6@crim12b.nsls.bnl.gov> NNTP-Posting-Host: crim12b.nsls.bnl.gov Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (X11; I; IRIX 5.3 IP17) Beamline X12B of the National Synchrotron Light Source, Brookhaven National Laboratory is open to all interested parties for purposes of macromolecular crystallography and time-resolved or static small angle diffraction. Beamline capabilities and application information are provided at URL: http://crim12b.nsls.bnl.gov/x12b_info.html Malcolm Capel Biology Dept. Brookhaven National Laboratory Upton, NY capel@crim12b.nsls.bnl.gov From owner-biophysics@net.bio.net Mon Nov 18 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!news.service.uci.edu!cchh.bio.uci.edu!user From: cchughes@uci.edu (Chris Hughes) Newsgroups: bionet.cellbiol,bionet.biophysics Subject: reversible disruption of nuclear membranes? Date: Mon, 18 Nov 1996 19:05:48 -0800 Organization: UCI Lines: 15 Message-ID: NNTP-Posting-Host: cchh.bio.uci.edu Xref: biosci bionet.cellbiol:5980 bionet.biophysics:2428 -- I'm looking for a drug that will reversibly disrupt (disassemble) nuclear membranes so as to mimic that point in the cell cycle where the "cytoplasm" and "nucleus" are contiguous. Does anyone know of such a drug, and if so where one can purchase it? Thanks, Chris Hughes -- Dr. Chris C.W. Hughes Dept. Mol. Biol. & Biochem. Biol. Sci. II Univ. California, Irvine cchughes@uci.edu Irvine CA 92717 ph (714) 824 8771 From owner-biophysics@net.bio.net Wed Nov 20 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!surfnet.nl!swidir.switch.ch!serra.unipi.it!sirio.cineca.it!gopher From: Stephen Roper Newsgroups: bionet.biophysics Subject: prova Date: Thu, 21 Nov 1996 16:49:18 -0800 Organization: Cineca Lines: 1 Message-ID: <3294F88E.2A99@newssun.med.miami.edu> Reply-To: sroper@med.miami.edu NNTP-Posting-Host: bio44.unimo.it Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Win16; I) prova From owner-biophysics@net.bio.net Wed Nov 20 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.sprintlink.net!news-peer.sprintlink.net!news-peer.gsl.net!news.gsl.net!news-dc.gsl.net!news.gsl.net!news.NetVision.net.il!news From: "R. Klein" Newsgroups: bionet.biophysics Subject: FREE pH measurement booklet Date: 21 Nov 1996 07:46:04 GMT Organization: NetVision LTD. Lines: 9 Message-ID: <5711bs$l7p@news.NetVision.net.il> NNTP-Posting-Host: ts002p8.pop2a.netvision.net.il Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) A free pH booklet is available which contains valuable information on basic pH measurement theory, pH measurement techniques, selecting the proper pH electrode for a particular application, and a pH troubleshooting guide. The booklet is available from Lazar Research Labs. Inc. by emailing service@lazarlab.com or faxing 1-213-931-1434. The booklet can also be obtained from the Lazar web site at http://www.lazarlab.com From owner-biophysics@net.bio.net Thu Nov 21 22:00:00 1996 Path: biosci!rutgers!gatech!csulb.edu!hammer.uoregon.edu!hunter.premier.net!news.mathworks.com!howland.erols.net!agate!usenet From: ed@wells.com (Ed) Newsgroups: alt.paranet.metaphysics,bionet.biophysics,sci.physics,sci.phyisics.accelerators,sci.phyisics.cond-matter Subject: buy your tech books at Cody's on-line Date: Fri, 22 Nov 1996 19:32:57 GMT Organization: University of California, Berkeley Lines: 26 Message-ID: <574uss$fh1@agate.berkeley.edu> NNTP-Posting-Host: haas-s300s009.haas.berkeley.edu X-Newsreader: Forte Free Agent 1.0.82 Xref: biosci bionet.biophysics:2436 sci.physics:180176 The best supply of technical and science books is now available at Cody's Books, one of the largest independent bookstores in world, based in Berkeley, CA. Cody's has the most complete section of computer books, programming books, books on the life sciences, engineering texts, and more. If you need a book on SQL, Chi-Squared Statistics, Spacetime Physics, or cold fusion then come to fusion. please feel free to mail order any book at: http://www.codysbooks.com/ Unlike most on-line book shops, Cody's Books is a real store with real knowledgeable people. If you have any book questions, you can call us or e-mail and we'll get back to you right away. Of course, you will always find the best prices with the best service at Cody's. So be sure to check us out. - Ed http://www.codysbooks.com/ p.s. We wish you the utmost reading pleasure. From owner-biophysics@net.bio.net Thu Nov 21 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!surfnet.nl!swidir.switch.ch!news!news!nntp Newsgroups: bionet.biophysics Subject: Re: fa$t ca$h on the net! Message-ID: <32959BEB.7309@IMT.UniNe.Ch> From: Bas de Heij Date: Fri, 22 Nov 1996 13:26:19 +0100 References: <3293851D.54CF@milwaukee.tec.wi.us> Organization: Institute of Microtechnology Nntp-Posting-Host: pc35-75.unine.ch X-Mailer: Mozilla 3.0 (Win95; I) MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 1 Some people try to work here !!!! From owner-biophysics@net.bio.net Fri Nov 22 22:00:00 1996 Path: biosci!agate!howland.erols.net!feed1.news.erols.com!phase2.worldnet.att.net!uunet!in1.uu.net!Gamma.RU!srcc!demos!demos2!dnews-server From: "RUMick" Newsgroups: bionet.biophysics Subject: test Date: 23 Nov 1996 14:25:21 GMT Organization: Home Sweet Home Lines: 7 Message-ID: <01bbd94a$50217d80$a45c55c2@alext.transit.ru> NNTP-Posting-Host: dialup-164.transit.ru Mime-Version: 1.0 Content-Type: text/plain; charset=KOI8-R Content-Transfer-Encoding: 7bit X-Newsreader: Microsoft Internet News 4.70.1155 test -- RUMick "The poor have sometimes objected to being governed badly; the rich have always objected to being governed at all." G. K. Chesterton (1874-1936) From owner-biophysics@net.bio.net Sun Nov 24 22:00:00 1996 Path: biosci!agate!howland.erols.net!news.sprintlink.net!news-peer.sprintlink.net!EU.net!usenet2.news.uk.psi.net!uknet!usenet1.news.uk.psi.net!uknet!dispatch.news.demon.net!demon!storepds.demon.co.uk From: paul@storepds.demon.co.uk (PDS) Newsgroups: bionet.biophysics Subject: An easy question Date: Mon, 25 Nov 1996 23:05:59 GMT Organization: Chaotic Lines: 10 Message-ID: <329a2599.734464@news.demon.co.uk> Reply-To: paul@storepds.demon.co.uk NNTP-Posting-Host: storepds.demon.co.uk X-NNTP-Posting-Host: storepds.demon.co.uk X-Newsreader: Forte Agent .99f/32.299 MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Can anybody tell me what the largest human organ is? PLEASE e-mail with the answer. Thanks for your time. Nigel Nigel@storepds.demon.co.uk From owner-biophysics@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!agate!howland.erols.net!feed1.news.erols.com!news.idt.net!enews.sgi.com!news.sgi.com!esiee.fr!jussieu.fr!univ-lyon1.fr!news From: Catherine Godinot Newsgroups: bionet.biophysics Subject: Concentration de l'oxygne Date: 26 Nov 1996 08:10:36 GMT Organization: UCB LYON I Lines: 6 Message-ID: <57e8ls$h7d@tempo.univ-lyon1.fr> NNTP-Posting-Host: mac-godind.univ-lyon1.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: godinot@univ-lyon1.fr X-URL: news:bionet.biophysics Could anyone tell me what is the oxygen concentration in a solution when the altitude is about 4000 meters? Thanks for your answers. Catherine From owner-biophysics@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!agate!spool.mu.edu!newspump.sol.net!howland.erols.net!feed1.news.erols.com!news.idt.net!enews.sgi.com!news.sgi.com!esiee.fr!jussieu.fr!univ-lyon1.fr!news From: Catherine Godinot Newsgroups: bionet.biophysics Subject: Concentration de l'oxygne Date: 26 Nov 1996 08:10:17 GMT Organization: UCB LYON I Lines: 6 Message-ID: <57e8l9$h7d@tempo.univ-lyon1.fr> NNTP-Posting-Host: mac-godind.univ-lyon1.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: godinot@univ-lyon1.fr X-URL: news:bionet.biophysics Could anyone tell me what is the oxygen concentration in a solution when the altitude is about 4000 meters? Thanks for your answers. Catherine From owner-biophysics@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!HSC.USC.EDU!meiselma From: meiselma@HSC.USC.EDU (Herbert J Meiselman) Newsgroups: bionet.biophysics Subject: Academic freedom and tenure at USC Date: 26 Nov 1996 14:02:59 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 254 Sender: daemon@net.bio.net Distribution: world Message-ID: <1.5.4.32.19961126220411.006c6c64@hsc.usc.edu> NNTP-Posting-Host: net.bio.net --=====================_849074651==_ Content-Type: text/plain; charset="us-ascii" Hello to all: At the suggestion of Ms. Carol Gross I am writing to request that the following two news items be distributed via the biophysics discussion newsgroup (bionet.biophysics). As you will note, the two items relate to a breach of contract action by the University of Southern California (USC) and the loss of tenure by Basic Science Faculty at the USC School of Medicine. The first item is the story about the law suit which was reported in the USC newspaper The Daily Trojan; the second is a response by the Provost which appeared in the medical school public relations paper called HSC Weekly. As you can see, the Provost does not even call for an attempt to resolve the problem---merely resorts to verbal attacks and name calling. As a long-standing member of the Biophysical Society I think this material should be of interest to all of our members. Loss of tenure and academic freedom at one institution is a serious matter that could affect many others! Please feel free to contact me if more information is needed or if the attachments are not in the correct form. 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c,L!J8'9bE@PcFfP[EL"dE`ebCA"bD@jd)'&bG'PME'9c)(GTG'JJBA4dFQPLGA4 TEfiJDA-JCR*PC@aj)'GTGQ9Z,L!J6fCQD@0PFb"KFQ8JE'pMBA4PC#"KG!da0$8 `)&0KEL"3B@*XEb"6G#iX)%4&55!b06%`,#"-Eh-J3@jRC@aPFb`J3d%J16!`-c- Z)#"&C'PdEh)k)#"+EfiJ6Q&XD@0V,Jdb-6-Y-c3b,6)i-c!X)'CKH#!p)$)a-bd c0$)Y-MJc-LiJ)'9YB@PX1L!JD(0MGfYXH8"SFf-ZGA0M,Q9NG5iJ9eGA1JeSG(4 `1Lm[GhGh,R9cBbjPC(8[D(0M,fPZCQm[F()[$3d0CQpbGf&bC'9N)'*j1Jd0$5S U+LSU+LSU$84b,L")BA*fCANJ8LiJ5f&cE'ph$8&cFfpMD@&dC5"3FQpQCA0cEh) JEfBJ8'KjFfP[E'pRH5"KEQ3J3QP[F'KjFfPMF`e9EQPfCA*cDA4j)'pQ)&0[GA4 SCA*Z)%0KE'PQEh*ZD@%J8f0SEfpX)'pQ)%ePC'PMD@jP$68`1#"0G@4N)%ePE@p bD@&X)&*PFf9KFQ0S)%*eD@aND@jR)#K069)Y06!i+3da-c-c)&0KEL"3B@*XEb" 6G(*PCA3X)%a[Fb""EQGPE'9c,#"$35!j-$!c-`e@EfPMC5!p)$)a-bdc0$)Y-6) d0#!JC@eKD@`J25"SDf&cE'ph)%"SFf-ZGA0M,Q9NG3e%DA*PBh3JCQ&i)#!b-6- Y-c3b,6)b068J)#"%CA"d,L"QBAJJ25!b-6-Y-c3b,6)b1$-0$3d0Hmd!!!: --=====================_849074651==_-- From owner-biophysics@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.sprintlink.net!news-peer.sprintlink.net!news-peer.gsl.net!news.gsl.net!news-dc.gsl.net!news.gsl.net!news.NetVision.net.il!news From: Steven Lemore Newsgroups: bionet.biophysics Subject: pH Measurement of Small Biological Samples Date: 26 Nov 1996 19:26:12 GMT Organization: NetVision LTD. Lines: 14 Message-ID: <57fg8k$ahk@news.NetVision.net.il> NNTP-Posting-Host: ts001p10.pop2a.netvision.net.il Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) The need frequently arrises in biological laboratories to measure pH in small sample vessels such as 96 well plates, microcentrifuge tubes, or NMR tubes. Standard pH electrodes are too large to fit into these small containers. Miniature glass electrodes are both breakable and have a high electrical resistance which makes the pH measurements unstable. A micro combination pH electrode has been developed which has a 1 mm tip and a 2 mm diameter flexible Teflon body which can measure pH in less less than 20 microliters of sample. This probe has a solid state sensor which gives drift free pH measurements. The probe can be used with a standard pH meter. Further information can be obtained via email at service@lazarlab.com From owner-biophysics@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!rutgers!uwm.edu!fnnews.fnal.gov!news.eng.convex.com!news.ecn.uoknor.edu!paladin.american.edu!news.jhu.edu!news From: Ronald Koder Jr Newsgroups: bionet.biophysics Subject: operon searching? Date: Tue, 26 Nov 1996 03:19:43 -0800 Organization: JHU Lines: 14 Message-ID: <329AD24F.41C6@intrepid.chm.jhu.edu> NNTP-Posting-Host: 128.220.23.72 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (X11; I; IRIX 6.2 IP20) This might be a little off topic (at least it's not a money making scheme :) ) Given an e. coli gene sequence, how do you go about finding out if it's in an operon, and if so, what other genes are in there with it? Is there some kind of PDB-type database with this info? -- -ron 'if I can't dance, I don't want to be a part of your revolution' Emma Goldman From owner-biophysics@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!online.no!sn.no!www.nntp.primenet.com!nntp.primenet.com!dciteleport.com!newsfeed.internetmci.com!hunter.premier.net!feed1.news.erols.com!news.idt.net!enews.sgi.com!news.sgi.com!esiee.fr!jussieu.fr!univ-angers.fr!ciril.fr!news.imag.fr!univ-lyon1.fr!news From: Catherine Godinot Newsgroups: bionet.biophysics Subject: oxygen concentration in altitude Date: 26 Nov 1996 08:50:42 GMT Organization: UCB LYON I Lines: 6 Message-ID: <57eb12$h7d@tempo.univ-lyon1.fr> NNTP-Posting-Host: mac-godind.univ-lyon1.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: godinot@cismsun.univ-lyon1.fr X-URL: news:bionet.biophysics Could anyone tell me what is the oxygen concentration in a solution when the altitude is about 4000 meters? Thanks for your answers. Catherine From owner-biophysics@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!univ-lyon1.fr!news From: Catherine Godinot Newsgroups: bionet.biophysics Subject: oxygen concentration in altitude Date: 26 Nov 1996 08:19:54 GMT Organization: UCB LYON I Lines: 6 Message-ID: <57e97a$h7d@tempo.univ-lyon1.fr> NNTP-Posting-Host: mac-godind.univ-lyon1.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: godinot@cismsun.univ-lyon1.fr X-URL: news:bionet.biophysics Could anyone tell me what is the oxygen concentration in a solution when the altitude is about 4000 meters? Thanks for your answers. Catherine From owner-biophysics@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!news-stkh.gsl.net!news.gsl.net!news-paris.gsl.net!news.gsl.net!news-peer.gsl.net!news.gsl.net!newspump.sol.net!howland.erols.net!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.idt.net!enews.sgi.com!news.corp.sgi.com!news.sgi.com!esiee.fr!jussieu.fr!univ-lyon1.fr!news From: Catherine Godinot Newsgroups: bionet.biophysics Subject: oxygen concentration in altitude Date: 26 Nov 1996 08:18:34 GMT Organization: UCB LYON I Lines: 6 Message-ID: <57e94q$h7d@tempo.univ-lyon1.fr> NNTP-Posting-Host: mac-godind.univ-lyon1.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: godinot@univ-lyon1.fr X-URL: news:bionet.biophysics Could anyone tell me what is the oxygen concentration in a solution when the altitude is about 4000 meters? Thanks for your answers. Catherine From owner-biophysics@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!online.no!sn.no!www.nntp.primenet.com!nntp.primenet.com!dciteleport.com!feed1.news.erols.com!news.idt.net!enews.sgi.com!news.sgi.com!esiee.fr!jussieu.fr!univ-lyon1.fr!news From: Catherine Godinot Newsgroups: bionet.biophysics Subject: Concentration de l'oxygne Date: 26 Nov 1996 08:17:40 GMT Organization: UCB LYON I Lines: 6 Message-ID: <57e934$h7d@tempo.univ-lyon1.fr> NNTP-Posting-Host: mac-godind.univ-lyon1.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: godinot@univ-lyon1.fr X-URL: news:bionet.biophysics Could anyone tell me what is the oxygen concentration in a solution when the altitude is about 4000 meters? Thanks for your answers. Catherine From owner-biophysics@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!online.no!sn.no!news-stkh.gsl.net!news.gsl.net!news-paris.gsl.net!news.gsl.net!news-peer.gsl.net!news.gsl.net!ix.netcom.com!enews.sgi.com!news.sgi.com!esiee.fr!jussieu.fr!univ-lyon1.fr!news From: Catherine Godinot Newsgroups: bionet.biophysics Subject: Concentration de l'oxygne Date: 26 Nov 1996 08:11:17 GMT Organization: UCB LYON I Lines: 6 Message-ID: <57e8n5$h7d@tempo.univ-lyon1.fr> NNTP-Posting-Host: mac-godind.univ-lyon1.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: godinot@univ-lyon1.fr X-URL: news:bionet.biophysics Could anyone tell me what is the oxygen concentration in a solution when the altitude is about 4000 meters? Thanks for your answers. Catherine From owner-biophysics@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!agate!howland.erols.net!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.idt.net!enews.sgi.com!news.sgi.com!esiee.fr!jussieu.fr!univ-lyon1.fr!news From: Catherine Godinot Newsgroups: bionet.biophysics Subject: Concentration de l'oxygne Date: 26 Nov 1996 08:13:36 GMT Organization: UCB LYON I Lines: 6 Message-ID: <57e8rg$h7d@tempo.univ-lyon1.fr> NNTP-Posting-Host: mac-godind.univ-lyon1.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: godinot@univ-lyon1.fr X-URL: news:bionet.biophysics Could anyone tell me what is the oxygen concentration in a solution when the altitude is about 4000 meters? Thanks for your answers. Catherine From owner-biophysics@net.bio.net Tue Nov 26 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!torn!ccshst05.cs.uoguelph.ca!ccshst01!cfares From: cfares@uoguelph.ca (Christophe Fares) Newsgroups: bionet.biophysics Subject: Room sharing at Biophys. Soc. Meeting in New Orleans Date: 27 Nov 1996 17:05:38 GMT Organization: University of Guelph Lines: 21 Message-ID: <57hsd2$84r@ccshst05.cs.uoguelph.ca> NNTP-Posting-Host: ccshst01.cs.uoguelph.ca X-Newsreader: TIN [version 1.2 PL2] Is there anyone out there going to the 41st annual Biophysical Society Meeting in New Orleans who would like to share a hotel room for the duration of the meeting? -- Christophe Fares, student, male.... \\/// (.. ) -----------------------o00-(_)--00o--------- |Address:Physics Dept. | | MacNaughton Bldg (Rm 014) | | University of Guelph | | Guelph, Ontario, CANADA | | N1G 2W1 | |Phone: (519)-824-4120 X8542 or X8541(lab) | |FAX: (519)-836-9967 | |E-mail: cfares@uoguelph.ca | -------------------------------------------- From owner-biophysics@net.bio.net Tue Nov 26 22:00:00 1996 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!howland.erols.net!newsfeed.internetmci.com!news-feed.iguide.com!delphi.com!usenet From: Harland Harrison Newsgroups: bionet.biophysics Subject: Re: An easy question Date: Wed, 27 Nov 96 12:04:33 -0500 Organization: Delphi (info@delphi.com email, 800-695-4005 voice) Lines: 1 Message-ID: References: <329a2599.734464@news.demon.co.uk> NNTP-Posting-Host: bos1b.delphi.com X-To: PDS Does the skin count as an organ? From owner-biophysics@net.bio.net Tue Nov 26 22:00:00 1996 Path: biosci!agate!spool.mu.edu!uwm.edu!news.cse.psu.edu!news3.cac.psu.edu!howland.erols.net!swrinde!ihnp4.ucsd.edu!scripps.edu!idefix!ketchemr From: ketchemr@idefix (Randal R. Ketchem) Newsgroups: bionet.biophysics Subject: Re: An easy question Date: 27 Nov 1996 08:39:45 GMT Organization: TSRI Lines: 4 Message-ID: <57guoh$86f@riscsm.scripps.edu> References: <329a2599.734464@news.demon.co.uk> NNTP-Posting-Host: idefix.scripps.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Newsreader: TIN [version 1.2 PL2] PDS (paul@storepds.demon.co.uk) wrote: : Can anybody tell me what the largest human organ is? Skin. From owner-biophysics@net.bio.net Tue Nov 26 22:00:00 1996 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!news.sprintlink.net!news-peer.sprintlink.net!su-news-hub1.bbnplanet.com!news.bbnplanet.com!cpk-news-hub1.bbnplanet.com!portc02.blue.aol.com!audrey01.news.aol.com!not-for-mail From: cluering@aol.com Newsgroups: bionet.biophysics Subject: Chlorophyll measurement Date: 27 Nov 1996 19:36:18 GMT Organization: AOL Bertelsmann Online GmbH & Co. KG http://www.germany.aol.com Lines: 90 Message-ID: <19961127193800.OAA02809@ladder01.news.aol.com> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com There is a new instrument for Chlorophyllanalysis available: The 1-Hz-Cuvette-Fluorometer An apparatus for the universal measurement of chlorophyll concentration. + integrated fast test for algae + determination of active chlorophyll The 1-Hz-Cuvette-Fluorometer offers a fast chlorophyll analysis in the laboratory. The chlorophyll analysis consists of the following: => The chlorophyll determination replaces the wet-chemical analysis. Different coloured LCDs assure that the chlorophyll content of all algae classes is assessed correctly. => The transmission is measured. => The activity of the algae is determined. The fluorescence signals fo, f, fm and the 1-Hz- method can be used for determining the activity. Fields of application: The apparatus can be used in the following fields: Fields of application: The apparatus can be used in the following fields: => water control => limnological work => environmental control => research and education => inflow control => chemical assessment => hazardous waste inquiries => river dam control Measurements: => Measurement of direct chlorophyll fluorescence: This measurement does not need sample preparation and is faster than the wet-chemical chlorophyll analysis to which it corresponds. It only takes 3 minutes. => Measurement of the living chlorophyll fluorescence in the sample (1-Hz-method): This measurement shows the percent of active chlorophyll and provides for the distinction between living chlorophyll and other substances which emit fluorescence. => Measurement of the Genty-parameter, as well as fm, f, fo: These methods can be used to measure the oxygen production rate. => Measuring the transmission of the sample: This measurement takes place automatically during each analysis so as to compensate the influence of substances which cause turbidity. The transmission can also be determined separately. => Toxicity tests (option): This test allows for a fast sample screening on algae-toxic substances. The measuring time is only 5 minutes. Additionally there is the possibility to examine the long-term influence of toxic substances. A program can be used which repeats the measurement in regular intervals. Equipment: => stationary in a laboratory or mobile (specially equipped) => integrated stirrer to hinder sinking of algae => auto-off => splash-proof housing => Power supplier 12V => RS232 for the connection to an external PC and printer => LCD-Display with graphic display of results Technical data: Measuring range 5ng - 500microg/l Weight 11kg Size (hxwxd) 220x370x400 mm Accumulators (Option) 2 x 6 Volt Voltage 12-14V= Halogen light 12 V/20W35mm( Sample temperature 0 - 30C Sample volume 25ml Data capacity 1000 datasets Intersection 2 x RS232 If you are interested please contact: bbe MOLDAENKE Schauenburgerstr. 116 D - 24118 Kiel Telefon + (0) 431/5 606 606 Telefax + (0) 431/5 606 609 BBE.Moldaenke@t-online.de From owner-biophysics@net.bio.net Wed Nov 27 22:00:00 1996 Path: biosci!GEOCITIES.COM!waynefullerton From: waynefullerton@GEOCITIES.COM (Wayne Fullerton) Newsgroups: bionet.biophysics Subject: Re: An easy question Date: 27 Nov 1996 16:29:30 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.16.19961127193254.281f8ebc@mail.geocities.com> NNTP-Posting-Host: net.bio.net >>Can anybody tell me what the largest human organ is? Well, Nigel, that's a more complex question that meets the eye. An organ is defined as a "colony" of cells acting as a unit to perform a certain given function -- like cardiac cells and the heart muscle. However, unlike the heart, some distinctions between organs are difficult, such as in the intestine. As a result, the body is usually classified in terms of organ systems, which contain serveral organs that perform a specific bodily function (i.e. nervous system, digestive, circulatory, etc.). That's a question I can answer -- skin is the largest organ system in (or should I say on ;-)! ) the body. IMO, the largest _individual_ organ is probably the large intestine. Get back to me BTW if anyone does e-mail the answer -- I'd be interested. From owner-biophysics@net.bio.net Wed Nov 27 22:00:00 1996 Newsgroups: bionet.biophysics Path: biosci!agate!howland.erols.net!vixen.cso.uiuc.edu!news.sprintlink.net!news-peer.sprintlink.net!uunet!in2.uu.net!128.100.1.3!utcsri!info.ecf!skule.ecf!dattani From: Roni S Dattani Subject: B-MODE COMP CAROTID ULTRASONAGRAPHY?? Sender: news@ecf.toronto.edu (News Administrator) Message-ID: X-Sender: dattani@skule.ecf Date: Thu, 28 Nov 1996 03:47:36 GMT X-Nntp-Posting-Host: skule.ecf Content-Type: TEXT/PLAIN; charset=US-ASCII Mime-Version: 1.0 Organization: University of Toronto, Engineering Computing Facility Lines: 30 Greetings all: Does anyone know of a WEBSITE where I can get some literature about the latest on the B-Mode Computerized Carotid Ultrasonagraphy?? This is a technique used to measure the thickness of the carotid walls (in vivo) and is useful in assessing probable risk to heart attacks.... If you don't have a web address handy but have know anything about it please post! Thankyou greatly in advance: ---oooo0----0oooo--------------------------------- ( ) ( ) "Sleep is good ..... \ ( ) / ...Especially when in school!" \_) (_/ Roni S. Dattani -Zzz DREAM-ON zzz! ENG SCI 9T8 (Biomed Eng.) -------------------------------------------------- From owner-biophysics@net.bio.net Thu Nov 28 22:00:00 1996 Path: biosci!unb.ca!savidge From: savidge@unb.ca ("Rod Savidge") Newsgroups: bionet.biophysics Subject: mass spectral libraries Date: 29 Nov 1996 05:54:32 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611290954093683.savidge@unb.ca> Reply-To: savidge@unb.ca NNTP-Posting-Host: net.bio.net Does anyone know whether the internet contains compilations of mass spectra, so that possible matches for submitted fragments can be obtained freely? ********************************************************************** Rod Savidge, PhD, Professor | E-mail: savidge@unb.ca Faculty of Forestry and \|/ Environmental Management \ | / Phone: (506) 453-4919 University of New Brunswick _\/ | \/_ Fredericton, NB CANADA \|/ Fax: (506) 453-3538 E3B 6C2 | From owner-biophysics@net.bio.net Sat Nov 30 22:00:00 1996 Newsgroups: bionet.biophysics Path: biosci!agate!nntpfeed.doc.ic.ac.uk!sunsite.doc.ic.ac.uk!lyra.csx.cam.ac.uk!warwick!yama.mcc.ac.uk!liv!lucs!rcp From: rcp@csc.liv.ac.uk (Ray Paton) Subject: IPCAT 97 - SECOND ANNOUNCEMENT AND CFP Sender: news@csc.liv.ac.uk (News Eater) Message-ID: Date: Fri, 29 Nov 1996 12:07:38 GMT Lines: 121 Nntp-Posting-Host: rcp@poulter.csc.liv.ac.uk Organization: Computer Science, Liverpool University SECOND ANNOUNCEMENT AND CALL FOR PAPERS - IPCAT '97 SECOND INTERNATIONAL WORKSHOP ON INFORMATION PROCESSING IN CELLS AND TISSUES IPCAT '97 SHEFFIELD, UK, September 1 - 4, 1997 PURPOSE OF IPCAT WORKSHOPS The purpose of the IPCAT workshop series is to bring together multidisciplinary groups of scientists working in the general area of modelling cells and tissues. A central theme will be the nature of biological information and the ways it is processed in cells and tissues. The workshop is intended to provide a forum to report research, discuss emerging topics and gain new insights into information processing systems, enzyme and gene networks, second messenger systems and signal transduction, automata models, PDP models, cellular automata models, single neuron computation, information processing in developmental systems, information processing in neural and non neural systems and new insights into non linear aspects of physiological behaviour. A key motivation is to provide a common ground for dialogue and reporting research without emphasising one particular research constituency or way of modelling or singular issue in this area. IPCAT '97 will seek to further the meaningful dialogue and exchangeof ideas started at IPCAT '95 between biologists, physicists, computer scientists and mathematicians. SUBMISSION DETAILS Papers should be 1.5 spaced in 12 point Times font. Maximum length of papers should be 12 pages. [Note: shorter contributions are also welcome]. 4 copies of papers should be submitted to: Ray Paton Department of Computer Science The University of Liverpool Liverpool L69 3BX UK Video and computer demonstrations are also welcome. PLEASE NOTE: The papers for the external proceedings will change according to publisher's style. IMPORTANT DATES Submission deadline ............................ February 15th 1997 Notification of acceptance ..................... April 20th 1997 Camera ready copies (internal proceedings) ..... May 20th 1997 Conference dates ............................... September 1st-4th 1997 Camera ready copies (published proceedings) .... October 5th 1997 WORKING LANGUAGE The working language of the Conference will be English which will be used for all printed material, presentations and discussion. INFORMATION ON IPCAT For further details about please see the IPCAT HomePage http://www.csc.liv.ac.uk/users/biocomp/iphome.htm or email tissues@csc.liv.ac.uk Please let us know your interest in the workshop by emailing Ray Paton at: tissues@csc.liv.ac.uk PUBLISHER Plenum CONFERENCE VENUE The meeting will take place in Halifax Hall (University of Sheffield). This is a former steel baron's mansion which has been extensively refurbished to provide high grade accommodation. It is located in Broomhill a leafy suburb of Sheffield about 10 minutes walk from the main University campus and 20 minutes from the city centre. INTERNATIONAL PROGRAMME COMMITTEE FOR IPCAT '97 Guenter Albrecht-Buehler (Cell Biology) - Northwestern University, Chicago Dennis Bray (Zoology) - University of Cambridge David Brown (Neurobiology) - Babraham Institute, Cambridge Robin Callard (Immunology) - University of London, London Chris Cannings (Statistics) - University of Sheffield Teresa Chay (Biological Sciences) - University of Pittsburgh Michael Conrad (Computer Science) - Wayne State University, Detroit John Easterby (Biochemistry) - University of Liverpool Bard Ermentrout (Mathematics) - University of Pittsburgh Leon Glass (Biological Sciences) - McGill University Albert Goldbeter (Nonlinear Systems) - University Libre, Brussels Uwe an der Heiden (Mathematics) - University of Witten/Herdecke Mike Holcombe (Computer Science) - University of Sheffield Felix Hong (Physiology) - Wayne State University, Detroit Tsuguchika Kaminuma (Biosciences) - NIHS, Tokyo George Kampis (Philosophy of Science) - Budapest Douglas Kell (Biochemistry) - University of Wales, Aberystwyth Rolf Koetter (Neurobiology) - University of Dusseldorf Gareth Leng (Physiology) - University of Edinburgh Philip Maini (Mathematics) - University of Oxford Pedro Marijuan (Electronics/BioInformatics) - University of Zaragoza Koichiro Matsuno (BioEngineering) - University of Nagaoka Hiroshi Okamoto (Neurobiology) - Fuji Xerox, Kanagawa Ray Paton (Computer Science) - University of Liverpool Peter Schuster (Molecular Biotechnology) - University of Jena Gordon Shepherd (Neurobiology) - Yale University Richard Stark (Mathematics) - University of South Florida, Tampa Rene Thomas (Molecular Biology) - University Libre, Brussels Chris Tofts (Computer Science) - University of Leeds Rickey Welch (Biochemistry) - University of Maryland Hans Westerhoff (Mathematical Biochemistry) - Free University, Amsterdam Gershom Zajicek (Medicine) - Hebrew University of Jerusalem From owner-biophysics@net.bio.net Sat Nov 30 22:00:00 1996 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!usc!newshub.csu.net!csulb.edu!hammer.uoregon.edu!arclight.uoregon.edu!news.bc.net!torn!hone!informer1.cis.McMaster.CA!roger.interlynx.net!usenet From: "Lars Thomsen" Newsgroups: bionet.biophysics Subject: Online single channel analysis. Date: 1 Dec 1996 21:02:15 GMT Organization: 115 South Oval Lines: 11 Message-ID: <01bbdfca$d7db9360$77c032cc@boris.gate2.interlynx.net> NNTP-Posting-Host: ppp09-max3.interlynx.net Dear Reader If you have some well explained sections of single channel analysis. Then please mail it to me and I will make appropriate figures and post it on my web page (with reference to you). You can take a look on my web page at : http://home.interlynx.net/~lthomsen/index.htm Best Regards Lars Thomsen