From owner-biophysics@net.bio.net Mon Dec 02 22:00:00 1996 Path: biosci!129.137.122.97!koehlekr From: koehlekr@129.137.122.97 (Ken Koehler) Newsgroups: bionet.biophysics Subject: Freshman Biophysics Text Date: 3 Dec 1996 06:28:34 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 32 Sender: daemon@net.bio.net Distribution: world Message-ID: <199612031427.GAA22895@net.bio.net> NNTP-Posting-Host: net.bio.net Greetings- I am in my third year of teaching a 30 week, algebra-based freshman physics course at the University of Cincinnati. The students who take this course are almost exclusively majoring in health-science or related fields. Three years ago I decided, in order to bring more relevancy and curricular focus to the course, to incorporate as many topics and examples as possible from human physiology. Hence the somewhat cheeky subject line. I have put together a hypertextbook which my students use for the course. It covers most of the major topics covered in a more traditional text, has 89 line drawings and graphs, links to 13 Mathematica notebooks (which the students use for problems, projects, simulations and labs) and 15 java programs which generate practice problems. The hypertext is located at http://ucrwcu.rwc.uc.edu/koehler/biophys/text.html I invite you to take a look at it and use any or all of it in whatever way it can help you. I must warn you that it is a bit eclectic, but the students who have used it to date are quite enthusiastic about both the material and its usefulness as they progress toward their degrees. I only ask that you send to me any comments, criticisms, corrections, suggestions or questions you have about it. My e-mail address is kenneth.koehler@uc.edu Thank you for your kind attention. Ken From owner-biophysics@net.bio.net Mon Dec 02 22:00:00 1996 Path: biosci!UCRWCU.RWC.UC.EDU!KOEHLEKR From: KOEHLEKR@UCRWCU.RWC.UC.EDU Newsgroups: bionet.biophysics Subject: Freshman Biophysics Text Date: 3 Dec 1996 07:51:37 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: <961203105027.45094@UCRWCU.RWC.UC.EDU> NNTP-Posting-Host: net.bio.net Greetings- I am in my third year of teaching a 30 week, algebra-based freshman physics course at the University of Cincinnati. The students who take this course are almost exclusively majoring in health-science or related fields. Three years ago I decided, in order to bring more relevancy and curricular focus to the course, to incorporate as many topics and examples as possible from human physiology. Hence the somewhat cheeky subject line. I have put together a hypertextbook which my students use for the course. It covers most of the major topics covered in a more traditional text, has 89 line drawings and graphs, links to 13 Mathematica notebooks (which the students use for problems, projects, simulations and labs) and 15 java programs which generate practice problems. The hypertext is located at http://ucrwcu.rwc.uc.edu/koehler/biophys/text.html I invite you to take a look at it and use any or all of it in whatever way it can help you. I must warn you that it is a bit eclectic, but the students who have used it to date are quite enthusiastic about both the material and its usefulness as they progress toward their degrees. I only ask that you send to me any comments, criticisms, corrections, suggestions or questions you have about it. My e-mail address is kenneth.koehler@uc.edu Thank you for your kind attention. Ken From owner-biophysics@net.bio.net Tue Dec 03 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!cs.utexas.edu!howland.erols.net!newsfeed.internetmci.com!news1.bellglobal.com!freenet-news.carleton.ca!cunews!nott!hone!informer1.cis.McMaster.CA!roger.interlynx.net!usenet From: "Lars Thomsen" Newsgroups: bionet.biophysics Subject: Re: Online single channel analysis. Date: 4 Dec 1996 01:43:48 GMT Organization: 115 South Oval Lines: 6 Message-ID: <01bbe184$7fd4b640$32c032cc@boris.gate2.interlynx.net> References: <01bbdfca$d7db9360$77c032cc@boris.gate2.interlynx.net> NNTP-Posting-Host: ppp40-max1.interlynx.net X-Newsreader: Microsoft Internet News 4.70.1155 The Theory page is located at this direct page : http://home.interlynx.net/~lthomsen/theory.htm Best Regards Lars Thomsen From owner-biophysics@net.bio.net Tue Dec 03 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!news-peer.gsl.net!news.gsl.net!howland.erols.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!news1.bellglobal.com!freenet-news.carleton.ca!cunews!nott!hone!informer1.cis.McMaster.CA!roger.interlynx.net!usenet From: "Lars Thomsen" Newsgroups: bionet.biophysics Subject: Re: Freshman Biophysics Text Date: 4 Dec 1996 01:42:52 GMT Organization: 115 South Oval Lines: 21 Message-ID: <01bbe184$5e353aa0$32c032cc@boris.gate2.interlynx.net> References: <961203105027.45094@UCRWCU.RWC.UC.EDU> NNTP-Posting-Host: ppp40-max1.interlynx.net X-Newsreader: Microsoft Internet News 4.70.1155 KOEHLEKR@UCRWCU.RWC.UC.EDU wrote in article <961203105027.45094@UCRWCU.RWC.UC.EDU>... > Greetings- > I invite you to take a look at it and use any or all of it in whatever way it > can help you. I must warn you that it is a bit eclectic, but the students who > have used it to date are quite enthusiastic about both the material and its > usefulness as they progress toward their degrees. > > I only ask that you send to me any comments, criticisms, corrections, > suggestions or questions you have about it. My e-mail address is I understand your students.. it is indeed a very nice page. I have promptly included it in my bookmarks. Best Regards Lars Thomsen From owner-biophysics@net.bio.net Tue Dec 03 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!news-peer.gsl.net!news.gsl.net!news-dc.gsl.net!news.gsl.net!news.NetVision.net.il!news From: Martin Sanders Newsgroups: bionet.biophysics,bionet.microbiology Subject: Measure TEMPERATURE with your PC at low cost Date: 4 Dec 1996 18:04:28 GMT Organization: NetVision LTD. Lines: 9 Message-ID: <584efc$n4d@news.NetVision.net.il> NNTP-Posting-Host: ts002p12.pop2a.netvision.net.il Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) Xref: biosci bionet.biophysics:2468 bionet.microbiology:8060 You can now measure 8 independent thermocouples (C,E,J,K,T,R, & S types) simultaneously with your PC using either DOS or Windows at low cost. Temperature monitor plugs directly into serial port of PC for direct display of temperature vs. time in either tabular or graphic format with data storage to disk. The monitor comes complete with software program for PC. For further details contact Lazar Research Labs. Inc. at 1-800-824-2066 in the U.S. or email service@lazarlab.com or fax 1-213-931-1434. From owner-biophysics@net.bio.net Tue Dec 03 22:00:00 1996 Path: biosci!rutgers!uwm.edu!news-peer.gsl.net!news.gsl.net!howland.erols.net!surfnet.nl!swidir.switch.ch!serra.unipi.it!lina!moro From: moro@cli.di.unipi.it (Lorenzo Moro) Newsgroups: bionet.biophysics Subject: HELP in neurology! Date: 4 Dec 1996 17:16:33 GMT Organization: Dipartimento di Informatica, Universita' di Pisa Lines: 18 Distribution: world Message-ID: <584blh$1i26@serra.unipi.it> NNTP-Posting-Host: lina.cli.di.unipi.it X-Newsreader: TIN [version 1.2 PL2] I'm searching help to find a treatment for Francesco, age 29, who is affected by a progressive paralyzation of legs and arms, before upper limbs and now even lower limbs. In evolution since 1984, it may be a disease of the second neuron of movement or an alteration of encephalon in the frontal right side. He risks, without appropriate treatment, an extension of the disease to vital muscles. Till today nobody stabilized the real origin of the disease. The already made clinic examinations are in the web address http://www.cli.ci.unipi.it/~moro/ciccio/ciccio.html if there is somebody, who is able to give him help, may send a mail to moro@cli.di.unipi.it or to muscato@dipmat.unict.it thanks, Lorenzo From owner-biophysics@net.bio.net Wed Dec 04 22:00:00 1996 Path: biosci!lsr.co.uk!wh From: wh@lsr.co.uk (Bill Henderson) Newsgroups: bionet.biophysics Subject: Job Positions Available Date: 5 Dec 1996 00:33:04 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Sender: daemon@net.bio.net Distribution: world Message-ID: <199612050833.IAA27812@ns.cityscape.co.uk> NNTP-Posting-Host: net.bio.net We would like to alert list subscribers to the availability of several new key positions at Life Science Resources. We are an established, fast growing high-tech company based in Cambridge, UK, and in the USA, and involved in world-wide sales of computer-based imaging products for life sciences and medicine. We have openings for the following positions and would like to invite any interested parties to apply via post, fax or email. Applications Support Specialists are required for pre- and post-sales support, including implementation, training and configuration of equipment in customer laboratories. Experience at post-graduate level in biology experimental procedures and research in the areas of optical probe applications in life sciences is essential, including competence in image processing techniques. This position will involve some foreign travel and could for the right candidates provide the opportunity to work from the company's branch office in the USA on temporary assignment for up to 12 month periods, or in the UK office. Software Engineers are required for biomedical applications programming. We require expert and motivated individuals for development of real time data acquisition systems (particularly imaging) under Windows 95 and Windows NT. The job will involve integration of new technology hardware and software components into the company's range of biomedical research instruments. Visual C/C++ and MFC experience essential, and a grounding in biology/biotechnology would be extremely useful. In these positions, there are opportunities for both contractors and individuals looking for permanent employment. Please send CV in first instance to: Dr. W. Henderson Managing Director Life Science Resources Ltd. Abberley House Granham's Road Great Shelford Cambridge CB2 5LQ United Kingdom Tel 44-(0)1223 845836 Fax 44-(0)1223 840342 Email: wh@lsr.co.uk Our Web page contains details on the company's products and can be found on http://www.lsr.co.uk/lsr From owner-biophysics@net.bio.net Wed Dec 04 22:00:00 1996 Path: biosci!DST.BOLTZ.CS.CMU.EDU!Dave_Touretzky From: Dave_Touretzky@DST.BOLTZ.CS.CMU.EDU Newsgroups: bionet.biophysics Subject: graduate training: Center for the Neural Basis of Cognition Date: 4 Dec 1996 19:29:28 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 73 Sender: daemon@net.bio.net Distribution: world Message-ID: <23739.849756539@DST.BOLTZ.CS.CMU.EDU> Reply-To: Dave_Touretzky@cs.cmu.edu NNTP-Posting-Host: net.bio.net Graduate Training with the Center for the Neural Basis of Cognition The Center for the Neural Basis of Cognition offers interdisciplinary Ph.D. and postdoctoral training programs operated jointly with affiliated programs at Carnegie Mellon University and the University of Pittsburgh. Detailed information about these programs is available on our web site at http://www.cnbc.cmu.edu. The Center is dedicated to the study of the neural basis of cognitive processes including learning and memory, language and thought, perception, attention, and planning; to the study of the development of the neural substrate of these processes; to the study of disorders of these processes and their underlying neuropathology; and to the promotion of applications of the results of these studies to artificial intelligence, robotics, and medicine. CNBC students have access to some of the finest facilities for cognitive neuroscience research in the world: Positron Emission Tomography (PET) and Magnetic Resonance Imaging (MRI) scanners for functional brain imaging, neurophysiology laboratories for recording from brain slices and from anesthetized or awake, behaving animals, electron and confocal microscopes for structural imaging, high performance computing facilities including an in-house supercomputer for neural modeling and image analysis, and patient populations for neuropsychological studies. Students are admitted jointly to a home department and the CNBC Training Program. Applications are encouraged from students with interests in biology, neuroscience psychology, engineering, physics, mathematics, computer science, or robotics. For a brochure describing the program and application materials, contact us at the following address: Center for the Neural Basis of Cognition 115 Mellon Institute 4400 Fifth Avenue Pittsburgh, PA 15213 Tel. (412) 268-4000. Fax: (412) 268-5060 email: cnbc-admissions@cnbc.cmu.edu The affiliated PhD programs at the two universities are: Carnegie Mellon University of Pittsburgh Biological Sciences Mathematics Computer Science Neurobiology Psychology Neuroscience Robotics Psychology The CNBC training faculty includes: German Barrionuevo (Pitt Neuroscience): LTP in hippocampal slice Marlene Behrmann (CMU Psychology): spatial representations in parietal cortex Pat Carpenter (CMU Psychology): mental imagery, language, and problem solving Jonathan Cohen (CMU Psychology): schizophrenia; dopamine and attention Carol Colby (Pitt Neuroscience): spatial reps. in primate parietal cortex Bard Ermentrout (Pitt Mathematics): oscillations in neural systems Julie Fiez (Pitt Psychology): fMRI studies of language John Horn (Pitt Neurobiology): synaptic learning in invertebrates Allen Humphrey (Pitt Neurobiology): motion processing in primary visual cortex Marcel Just (CMU Psychology): visual thinking, language comprehension Eric Klann (Pitt Neuroscience): hippocampal LTP and LTD Alan Koretsky (CMU Biological Sciences): new fMRI techniques for brain imaging Tai Sing Lee (CMU Comp. Sci.): primate visual cortex; computer vision David Lewis (Pitt Neuroscience): anatomy of frontal cortex James McClelland (CMU Psychology): connectionist models of cognition Carl Olson (CNBC): spatial representations in primate frontal cortex David Plaut (CMU Psychology): connectionist models of reading Michael Pogue-Geile (Pitt Psychology): development of schizophrenia John Pollock (CMU Biological Sci.): neurodevelopment of the fly visual system Walter Schneider (Pitt Psych.): fMRI, models of attention & skill acquisition Charles Scudder (Pitt Neurobiology): motor learning in cerebellum Susan Sesack (Pitt Neuroscience): anatomy of the dopaminergic system Dan Simons (Pitt Neurobiology): sensory physiology of the cerebral cortex William Skaggs (Pitt Neuroscience): representations in rodent hippocampus David Touretzky (CMU Comp. Sci.): hippocampus, rat navigation, animal learning From owner-biophysics@net.bio.net Fri Dec 06 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!insync!news.io.com!usenet From: "Jason Spofford" Newsgroups: bionet.biophysics Subject: ANNOUCING: Primordial Life 3.1 Date: 7 Dec 1996 16:34:50 GMT Lines: 24 Message-ID: <01bbe45c$b1109a00$2759aac7@spofford.io.com> NNTP-Posting-Host: dialup-01-039.austin.io.com X-Newsreader: Microsoft Internet News 4.70.1155 Announcing Primordial Life 3.1 for Windows 95 and Windows NT New in Primordial Life 3.1 is the ability to connect your artificial life world to others over the Internet or intranet. Instead of "biots" (artificial life creatures) bouncing off walls, they can go through walls to other computers. Those computers, in turn, can connect to others creating a biot universe virtually unlimited in size. In general, Primordial Life evolves artificial life forms both in a screen saver and window. Right now, the program focuses on evolving form and motion. Future versions will include behavior. The computing requirements for evolving artificial life can be staggering. Now you can join forces with other artificial life enthusiasts to create larger worlds supporting a larger diversity of life forms. Primordial Life 3.1 is shareware. You can do most everything with the unregistered version. You can download Primordial Life from http://www.io.com/~spofford If you have any questions or comments, you can reach me at spofford@io.com From owner-biophysics@net.bio.net Sun Dec 08 22:00:00 1996 Path: biosci!CRYSTAL.IMBB.FORTH.GR!eva From: eva@CRYSTAL.IMBB.FORTH.GR ("Eva Scheuring") Newsgroups: bionet.biophysics Subject: "cookbook" for mutagenesis Date: 9 Dec 1996 08:12:16 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: <9612091811.ZM6573@crystal.imbb.forth.gr> NNTP-Posting-Host: net.bio.net Dear Netters, I would appreciate if someone could suggest an up-to-date intro guide (for a non-biologist) about mutagenesis. Thank you in advance! Sincerely, Eva Scheuring Vanamee, Ph.D. IMBB/University of Crete tel: +30-81-393143 Department of Biology fax: +30-81-239155 P.O. Box 2208 71409 Iraklion, Crete, Greece e-mail:eva@crystal.imbb.forth.gr -- From owner-biophysics@net.bio.net Mon Dec 09 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!feed1.news.erols.com!insync!Gamma.RU!srcc!news.uni-stuttgart.de!news.ruhr-uni-bochum.de!news.rhrz.uni-bonn.de!usenet From: Patrik HÖller Newsgroups: bionet.biophysics Subject: magnetic orientation Date: Tue, 10 Dec 1996 00:21:58 -0800 Organization: RHRZ - University of Bonn (Germany) Lines: 8 Message-ID: <32AD1DA6.5CF6@uni-bonn.de> NNTP-Posting-Host: rhrz-ts3-p15.rhrz.uni-bonn.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01DT [de] (Win16; I) Hi there, I am new here - so please tell me if I am digging at the wrong site: I am planning to do a research on amgnetic orientation in bats. For that purpose I am gathering any information concerning magnetic orientation in mammals (quite rare, I gues...) Thanks in advance to all of you wasting a few thoughts to my problem - - bye Patrik From owner-biophysics@net.bio.net Mon Dec 09 22:00:00 1996 Path: biosci!agate!howland.erols.net!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!su-news-hub1.bbnplanet.com!arclight.uoregon.edu!news.bc.net!rover.ucs.ualberta.ca!news.ucalgary.ca!news From: "Dr. L.W. Haynes" Newsgroups: bionet.biophysics Subject: Re: magnetic orientation Date: Tue, 10 Dec 1996 09:16:10 -0800 Organization: University of Calgary Lines: 20 Message-ID: <32AD9ADA.7387@acs.ucalgary.ca> References: <32AD1DA6.5CF6@uni-bonn.de> NNTP-Posting-Host: @hm107.meds.ucalgary.ca Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 3.0 (Win16; I) Patrik HÖller wrote: > > Hi there, > I am new here - so please tell me if I am digging at the wrong site: > I am planning to do a research on amgnetic orientation in bats. For that > purpose I am gathering any information concerning magnetic orientation in > mammals (quite rare, I gues...) > Thanks in advance to all of you wasting a few thoughts to my problem - - > bye > Patrik For a start, try the 45th Annual Symposium issue of the Society of General Physiologists "Sensory Transduction". Two articles may be of interest: S.M. Block "Biophysical principles of sensory transduction" and Kirshcvink et al. "Discrimination of low-frequency magnetic fields by honey-bees: biophysics and experimental tests". Tuna, pigeons, bees and a host of other critters are known to use magnetic fields for orientation purposes. L.W. Haynes Univ. of Calgary From owner-biophysics@net.bio.net Wed Dec 11 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: gsw@mol.biol.ethz.ch (Gerhard Wider) Newsgroups: bionet.biophysics Subject: POST-DOC Position in NMR Date: 10 Dec 1996 15:21:55 -0000 Lines: 34 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <58jv6j$enp@mserv1.dl.ac.uk> X-Sun-Charset: US-ASCII Original-To: biophys@dl.ac.uk POST-DOC POSITION IN BIOMOLECULAR NMR AT THE "ETH ZURICH" In the group of Prof. Kurt Wuthrich at the Swiss Federal Institute of Technology in Zurich, Switzerland, (ETH Zurich) a post-doctoral position is available for one year (renewable for up to 3 years). Applicants should have experience in modern NMR spectroscopy and a strong interest in technical aspects of NMR applied to biological macromolecules. The position has been funded for a project to optimize NMR experiments with aqueous samples at high salt concentrations. We operate four NMR machines: 400, 500, 600 and 750 MHZ. All instruments are of the newest design and fully equipped for all applications in high resolution biomolecular NMR. The position will be available immediately. Applications will be accepted until the position is filled. We thank all applicants in advance for their interest. Qualified individuals are invited to submit their resume, along with two letters of references to: Prof. Dr. Kurt Wuthrich Institute for Molecular Biology and Biophysics ETH Hoenggerberg CH-8093 Zurich / Switzerland Fax.: (..41) (1) 633 1151 www-homepage: http://www.mol.biol.ethz.ch/wuthrich/ From owner-biophysics@net.bio.net Wed Dec 11 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: thomas@cicada.berkeley.edu (Thomas Z. Lauritzen) Newsgroups: bionet.biophysics Subject: heat damage Date: 10 Dec 1996 01:55:29 -0000 Lines: 12 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <58ifuh$rcd@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk I would like to use a micromachined gripper for intravascular use, but it produces some heat while in function. Does anyone know how high a lokal temperature you can expose cells/ biological tissue to without damage? What is the heat conductance of whole blood? How big a mechanical force is needed to cut blood vessel epithelia from the intravascular side? Is there any litterature about this? Thanks Thomas Lauritzen thomas@cicada.berkeley.edu From owner-biophysics@net.bio.net Wed Dec 11 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!www.nntp.primenet.com!nntp.primenet.com!uwm.edu!rutgers!csn!nntp-xfer-1.csn.net!csn!nntp-xfer-2.csn.net!yuma!purdue!mozo.cc.purdue.edu!omni.cc.purdue.edu!rick From: rick@omni.cc.purdue.edu (Rick Millane) Newsgroups: bionet.biophysics Subject: Postdoctoral position: Computational crystallography Date: 11 Dec 1996 22:19:12 GMT Organization: Purdue University Lines: 26 Message-ID: <58nc10$50g@mozo.cc.purdue.edu> NNTP-Posting-Host: omni.cc.purdue.edu X-Newsreader: NN version 6.5.0 (NOV) POSTDOCTORAL POSITION COMPUTATIONAL CRYSTALLOGRAPHY Purdue University Whistler Center for Carbohydrate Research and Computational Science and Engineering Program West Lafayette, IN 47907-1160 Attention: Dr. R.P. Millane A postdoctoral position is available at Purdue University on projects concerned with the development of new computational algorithms for macromolecular crystallography. The primary current interest is in the development of new electron density modification algorithms for protein crystallography. Applicants should have a Ph.D. and experience in computational methods in x-ray crystallography, or in a related field. Experience with C programming, Unix and protein crystallography is desirable. Send cv, a summary of research experience and interests, and the names of three referees to Dr. Rick Millane, Whistler Center for Carbohydrate Research, and Computational Science and Engineering Program, Purdue University, West Lafayette, IN 47907-1160, tel. 317-494-9272, fax. 317-494-7953, rmillane@purdue.edu. From owner-biophysics@net.bio.net Wed Dec 11 22:00:00 1996 Path: biosci!rutgers!uwm.edu!www.nntp.primenet.com!nntp.primenet.com!news.sprintlink.net!news-peer.sprintlink.net!howland.erols.net!usenet.kornet.nm.kr!news.kreonet.re.kr!newsfeed.kreonet.re.kr!news.kaist.ac.kr!news From: "Lee, Sangjae" Newsgroups: bionet.biophysics Subject: What relationship? Date: Fri, 13 Dec 1996 00:09:19 +0000 Organization: Daelim Industrial CO. Ltd Lines: 9 Message-ID: <32B09EAF.4F42@magpie.daelimrnd.co.kr> Reply-To: sjlee@magpie.daelimrnd.co.kr NNTP-Posting-Host: db007.daelimrnd.co.kr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) Dear all. I would like to know what relationship between regeneration ability(or mechanism) and the way cells arrange. Web sites or references are welcome. Thank you for your help in advance. Sangjae From owner-biophysics@net.bio.net Thu Dec 12 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!www.nntp.primenet.com!nntp.primenet.com!news-peer.gsl.net!news.gsl.net!portc01.blue.aol.com!audrey01.news.aol.com!not-for-mail From: dhackos@aol.com (DHackos) Newsgroups: bionet.biophysics Subject: Solvents in patch clamping Date: 13 Dec 1996 02:55:00 GMT Organization: AOL http://www.aol.com Lines: 22 Message-ID: <19961213025400.VAA25934@ladder01.news.aol.com> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com I need to include a not so soluble molecule in the internal solution for a whole cell patch clamping experiment. I have been using DMSO as a solvent for this molecule, which I dilute into the intracellular solution to a final concentration of about 1%. I have found, unfortunately, that even such small amouts of DMSO cause problems when I try to fill the tip of a pipette, probably because DMSO makes the solution more hydrophobic. I have also tried DMF and acetonitrile. DMF is similar to DMSO (causing pipette filling problems), but acetonitrile causes no problems at all. On the other hand, I am worried about using acetonitrile in a whole cell experiment since I know of no one else who has ever done this. I would like to know if anyone else has had any experience using organic solvents in patch clapming experiments, and if so, which ones have you tried and with what success. Thanks in advance to anyone who can help, Dave Hackos Dept. of Physiology UC San Francisco From owner-biophysics@net.bio.net Fri Dec 13 22:00:00 1996 Path: biosci!rutgers!uwm.edu!www.nntp.primenet.com!nntp.primenet.com!howland.erols.net!newsfeed.internetmci.com!demos!news.telekom.ru!bsd.ropnet.ru!newsmaster From: "Oleg S. Vasilyev" Newsgroups: bionet.biophysics Subject: mathematical simulation in immunology Date: Sat, 14 Dec 1996 19:18:25 -0600 Organization: RopNet Lines: 16 Message-ID: <32B351E1.1CC8@bsd.ropnet.ru> Reply-To: mathmed@ropnet.ru NNTP-Posting-Host: mathmed.ropnet.ru. Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Win95; I) Dear sirs! I am occupied with preparation of the review on mathematical models in immunology for Russian scientific press. I shall be sincerely glad to include in this review the information on your researches in the field of mathematical simulation in immunology. Please, answer. Yours faithfully, Oleg S. Vasilyev. NATIONAL RESERCH CENTR-INSTITUTE OF IMMUNOLIOGY RUSSIA, MOSCOW mathmed@ropnet.ru From owner-biophysics@net.bio.net Sat Dec 14 22:00:00 1996 Path: biosci!RUBENS.ITS.UNIMELB.EDU.AU!bjo From: bjo@RUBENS.ITS.UNIMELB.EDU.AU ("Bernard O'Loughlin") Newsgroups: bionet.biophysics Subject: THE SIN AGAINST THE HOLY GHOST Date: 15 Dec 1996 07:06:25 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 287 Sender: daemon@net.bio.net Distribution: world Message-ID: <2.2.16.19961216014512.260f4f3e@RUBENS.ITS.UNIMELB.EDU.AU> NNTP-Posting-Host: net.bio.net THE SIN AGAINST THE HOLY GHOST BECAUSE OF ITS VILENESS, TORTURE SEX IS THE GREATEST CRIME AGAINST HUMANITY IMAGINABLE. BUT IT ISN'T THE UNFORGIVABLE SIN. IT JUST FEELS LIKE IT AFTERWARDS. SUMMARY: (i) The Biblical evidence for torture sex being the sin against the Holy Ghost is zilch. The sin is mentioned, but NOWHERE defined. (ii) The association turns basically on an interpretation by some within the Catholic Church, which has been enshrined in canon law. Canon law is fallible; it can and does change. And this interpretation is misguided, as many clergy within the Catholic Church would concede if presented with the following case. (iii) Consider a hitman who takes such great chances for a potential torture-sex victim that he ends up becoming a victim himself. Wouldn't God love such enormous courage? Wouldn't He be more concerned with what was in the heart than with what was on the history sheet? Hitmen can find salvation, and so can you, without necessarily showing such extreme courage. (iv) For once, BE somebody for the Universe, and not just a brainwashed, scar(r)ed, nasty little bunny rabbit. 1. WHAT IS THE AUTHORITY FOR THE "SIN AGAINST THE HOLY GHOST"? 2. IS TORTURE SEX THE SIN AGAINST THE HOLY GHOST? 3. SO WHAT IS THE SIN AGAINST THE HOLY GHOST? 4. WOULD THE LORD OF CHAOS BE INTERESTED? 5. ACHIEVING FORGIVENESS. 6. CAN INNOCENCE BE REGAINED? 7. THE AUTHOR'S STARTING AND FINISHING POINTS. 1. WHAT IS THE AUTHORITY FOR THE "SIN AGAINST THE HOLY GHOST"? The sin against the Holy Ghost (the "unforgivable sin") is mentioned only in passing in the three "synoptic" Gospels (Matthew 12:32, Mark 3:29 and Luke 12:10). (Synoptic, "with the one view", because all three Gospels came from basically the same sources.) It occupies only a verse in each case. John doesn't write about it at all. In other words, in the prime authorities of the New Testament, the Gospels, the discussion of the sin against the Holy Ghost is hardly central. (Check it out for yourself: http:// www.gospelcom.net/ bible) Is the sin against the Holy Ghost (called "blasphemy" in the King James Version) defined? No, it is not. So HOW did torture sex come to be thought of as the sin against the Holy Ghost? 2. IS TORTURE SEX THE SIN AGAINST THE HOLY GHOST? For the few mega-warped medieval "Christians" into torture sex, who felt themselves to be beyond redemption, the association between the famous passage of Paul about the body as the "temple of the Holy Ghost" (1 Corinthians 6) and the sin against the Holy Ghost was obvious. Mightn't the sin against the Holy Ghost possibly be...torture sex? Later, some senior people in the Catholic Church, aware of the warped ones, erroneously thought that it might be too. After all, it was the vilest of sins. Their quite fallible misinterpretation persists, especially for those on whom it was imprinted in a state of semi-hysterical gullibility. Now, of course, even if an association between the sin against the Holy Ghost and the body as the temple of the Holy Ghost might be plausible, that does not suggest torture sex in the slightest. In fact, reading the rest of the cited chapters, it might well be concluded with equal logic that, among other things, being an active gay was the sin against the Holy Ghost. Other passages of Scripture which might be cited as evidence are even more distantly related. The Biblical evidence that torture sex is the sin against the Holy Ghost is anything but compelling. It all turns on the internal "canon law" of the Catholic Church, which was purely reactive in this case. And Catholic canon laws are like ordinary laws: they might be enforceable, with certain "church" penalties, but equally they might be changed tomorrow. Canon law can and does change. It is not an immutable summary of God's eternal truths. The shallowness of the canon law interpretation in this instance is shown by consideration of a simple, but true, example. An expert hitman came to love a potential torture-sex victim as a friend. In attempting to protect him, the hitman became, almost inevitably, a victim himself. He knew what he had done in his life, he well knew what the likely penalties for defending his friend were, but he went ahead anyway. Who would dare suggest that God would reject such a one? Jesus said (of a simple death): "Greater love hath no man than this, that a man lay down his life for his friends" (John 15:13) and also: "Inasmuch as ye have done it [for] one of the least of these my brethren, ye have done it [for] me" (Matthew 25:40). The hitman's love and nearly unbelievable courage would have saved him, baptised him in blood. Nothing he could have been made to do or say in his last earthly moments could, because of its forced nature, conceivably have lessened his beauty before God. Indeed, his last free words, facing a horror beyond natural human description, were to request that no blood be splattered upon his jumper ("sweater"). It had been given to him by the friend and, although grubby from overuse on the run, symbolised their friendship. More importantly, the story of his final courage started to get around - as he knew it would - and helped protect his friend further. At a time when most would turn to jelly, with thoughts only of what was to come, he kept his cool enough to do the single most useful thing within his power to save another. That shows where his soul was at. So, even though the hitman had engaged in torture sex most of his life (apparently willingly), he had not committed the unforgivable sin. Even if that story were not true (and I hate that it is real, although I rejoice in his heroism and eternal reward), it certainly COULD be. There are people in this world with such enormous generosity of spirit, even amongst those who would normally hide self-hatred behind plausible bravado. And, just as the robber who hung on the cross beside Jesus, they will enjoy paradise (Luke 23:39-43). Only one negative case is needed to prove the point: torture sex is not the sin against the Holy Ghost. 3. SO WHAT IS THE SIN AGAINST THE HOLY GHOST? If Matthew, Mark and Luke didn't bother to define the sin against the Holy Ghost, some plausible hypotheses for that are: (a) it isn't important in general (hard to believe, since it denies salvation), (b) it is so uncommon that it's hardly worth talking about (well, perhaps then...), (c) they couldn't write an essay well enough to pass a first year Medicine exam (even with Dads who were Doctors), and (d) the readers/listeners for whom they were writing would have known straight away what they were talking about. The last alternative seems the most likely to me. Their readers/listeners KNEW what "blasphemy" (the sin) against the Holy Ghost was. And, indeed, tautological though it might be, it is pretty obvious. Quite simply, the sin against the Holy Ghost is refusing to believe in the love of God. The Holy Ghost is the spirit of God, the spirit of love, in the Godhead. "Blasphemy" against the Holy Ghost is denying the possibility of God's forgiveness. If you don't want it, you can't have it. The readers/listeners for whom they were writing, in the Palestine of the 1st Century AD, would have been very familiar with the Holy Ghost ("the comforter" in John) as the spirit of love. But would anyone seriously suggest that there and at that time torture sex was common? Maybe a badly beaten camel, or a bit of shit-maker in the well, perhaps even the odd "Murder of the Innocents", but widespread torture sex? Do me a favour! The reality of the sin against the Holy Ghost, however, is not just the simple denial of God's love once. By its very nature, hopelessness feeds upon itself. Feeling divorced forever from Universal forgiveness leads inexorably to an inward spiral of self-hatred and the perpetuation of self-hating action. Eternal hope is cut off more and more. Unbroken, the process will complete itself. Where Catholic canon law gets close to the mark is that torture sex is the most psychologically detrimental sin imaginable (barring the S. against the H.G.). Accordingly, torture sex is likely to lead to the (real) sin against the Holy Ghost more than anything else. But do you see the distinction? Torture sex ISN'T the sin against the Holy Ghost itself, it's just the most likely to give rise to the feelings that become the sin against the Holy Ghost. It can be forgiven, but not if hopelessness takes over to the point where the vision is clouded forever. 4. WOULD THE LORD OF CHAOS BE INTERESTED? Does Homer like donuts? What kind of Satan would want souls foist upon him? Surely the real Lord of Chaos would want people to choose him freely: raunchy chaos over pusillanimous order, that sort of thing. But on the rebound? Even Satan wants you to get it right about the sin against the Holy Ghost. Choose the Lord of Chaos, by all means, but CHOOSE. And choice requires freedom. Know that you haven't committed the sin against the Holy Ghost...yet. However, if you think about it, the Lord of Chaos would hate torture sex as much as God does. Since when is rotten, vile, ghastly, fucked-up, hypocritical and unnatural ORDER equivalent to the pure chaos of "evil"? Is Satan a hypocrite? Would he want such vile, hypocritical, followers? Such a fucked-up response to fucked-up medieval Christianity couldn't possibly describe the Lord of the Universe that I would want to serve. It would be as false to suggest that that was Satanism as it would be to put forward that the sale of indulgences was Christianity. Further, if Satan were to have a motto, it would surely be "give the cunts hell". Guess who the cunts are? Instead of being rewarded, those who continue to advocate such patently false Satanism will, if there are levels to hell, go straight to the bottom for eternity. They are traitors to God and Satan alike. 5. ACHIEVING FORGIVENESS. Even if torture sex isn't the sin against the Holy Ghost, it is vile beyond belief and the nastiest of sins. You may have been forced into it. You may have been brainwashed into thinking that it was nothing much. Even if you don't bear full responsibility for the vileness, though, it is likely that you will feel the need for forgiveness. The association between MY sexual pleasure and SOMEONE ELSE'S agony is too sick (too, too, sick) to avoid all personal consequences. It is natural to feel irretrievably dirty after such hideous and hateful unnaturalness, no matter what the pseudo-justifications offered by the tutor-cunts. Sometimes the heart knows what the head denies. In a technical spiritual sense, simply asking for forgiveness may be what actually achieves it. However, practically, forgiveness is not achieved, either spiritually or psychologically, by FAITH alone, but also by WORKS. "Wow, I am forgiven!" is not good enough by itself, soon running out of currency unless acted upon. As people are, so shall they act. Faith without works is fruitless, just as works without faith are empty. You need to DO something. Work, fight, in every way open to you against what the Universe hates. (Because of brainwashing, there are probably more ways open to you than you realise.) Doing "nice" things, by joining Rotary for instance, is not the way to go, except as a "cover" or as a temporary relief from spiritual directness. (Actions which of themselves might be laudable can, in the face of such treason, be reprehensible. Too, too, comfortable for words.) Direct action against the source of the rottenness itself is required, even if that might have to be covert much of the time. Don't put bandaids on the cancer - there are lots of people prepared to do that. Do everything within your power to kill the cancer. Does that mean that you have to show as much courage as the hitman in order to be forgiven? No, it doesn't. An appropriate analogy is that of canonisation - the making of a saint - within the Catholic Church. Canonisation means that the Church is CERTAIN that a particular person is in heaven. Lots of other people also make it, though, even if the Church on earth isn't certain of it. Similarly, great courage may create a certainty of reward, but lesser courage may be just as effective in reaching the desired goal. Do what you can, what you REALLY can. That means taking reasonable chances. It may seem that all the power, overwhelming power, is on the other side, but that power has limits. You have the Universe on your side, and ultimately it's the truth that counts as the source of power. Using your brains, you can subvert the rotten system and start achieving forgiveness. At the least, talk about how forgiveness is possible and can be earned to your friends who are in a similar quandary. There is no need to be one of those who apparently believe that nothing can be done, so one should enjoy it as much as possible, even if always terrified of death. Nor is there a need to be one of the more despicable, but piteous, hypocrites who apparently believe that there isn't even a problem, even if always terrified of death. Give your friends the chance to get off the merry-go-round of self-hatred, often masked and often clothed in "religion". Then, they might knowingly exercise the option to become real Christians, real Muslims, real Satanists or real anything-elses. They will love you as much as they are relieved, even if new responsibilities are introduced. 6. CAN INNOCENCE BE REGAINED? Unfortunately, no: innocence once lost can't be regained. It's a one-way process. It is possible, however, to (re)learn its near relative "naturalness", our dignity as natural animals (who could never be into torture sex). It is possible to free oneself to some extent from the false ways of an unnatural society. Going along with "naturalness" can be a unique and inestimable sort of "certainty-happiness". All that is required is that you respond without self-deception to the greatest nastiness in your life (and that it inevitably is, if rarely considered let alone acknowledged). That "certainty-happiness" will give you great strength and comfort, even if you can't possibly avoid having to give way to duress again. Bide your time, plot and plan your response, a response suited to your talents. If it is your way to serve, kill the main perpetrators. None of them is your friend. None of them is a "nice person". None of them is worthy of deference. All are the vilest of continuing, voluntary, murderers, traitors and criminals against humanity. The law as it is supposed to be would freely sanction such justifiable homicide. The Universe would love you. Just make sure that, to avoid retribution, you die in the act. 7. THE AUTHOR'S STARTING AND FINISHING POINTS Why am I interested in the sin against the Holy Ghost? Because the two people I've most loved - Phil, the hitman, and, because of him, Robbie - were both "caught" in torture sex, although I didn't know that from the beginning. Somehow, they were strong enough to understand that if another, natural, "animal" could really love them, then real love and forgiveness in the Universe were possible. They understood intuitively, if unusually, that the standard definition of the sin against the Holy Ghost was just wrong. The hardest thing is to forgive oneself. I wanted to help in that process in their honour. As this is being transmitted, I am in the process of "shuffling off this mortal coil", of joining "les justes". I was in danger already, and the publication of this would, if I were not to act first, seal my earthly fate with certainty. The martyrs would not demand pointless suffering. A protracted, agonising, death is neither mandatory nor inevitable. What matters is that one's death should count. (Are you listening, suicide bombers?) Witness enough is here in my little bit for Phil and Robbie, for you, against the greatest vileness in the history of the world. I hope that my "parvum opus" provides some of you with the thought-data and the courage to be even a tiny bit unwise in the face of that oh-so-socially-acceptable treason. You know, if most people of quality in the world were prepared to take a chance of 1 in 10, or even 1 in 100, their actions, along with the actions of some prepared to waive their right to a long life, would see history reversed. The human race could free itself forever from the systematic, institutionalised, hypocrisy and foulness which infect every level, and affect every aspect, of societies around the world. How could you expect to live in tune with the Universe, and die with equanimity, if you never did anything about such inhuman (and unsatanic) rottenness? That point explains really why there are so few people in the world who are genuinely, naturally, at-home with themselves, and why so many claw to hang on to their miserable little lives. It's tough being a nasty little bunny rabbit. Don't just warm yourself by the fire. Throw on some wood, and watch the cunts scatter. Translate, linguists! Republish freely, anonymously, cyber(un)holy! May the sun be always in your face, and the wind always at your back. ALLAHU AKBAR! __________________________________________ Bernard J. O'Loughlin, Ph.D., Psychologist Melbourne, Victoria, Australia, The World, The Galaxy, The Universe The views expressed in this document are mine alone, although the sources are many - the product of years of poaching with gratitude. NO-ONE ELSE knew of its contents, or of my intention to publish it, beforehand. Indeed, a lot of subterfuge has been used over the last year to achieve that. This article has been posted at the same time to dozens of mailing lists. *bows out* __________________________________________ From owner-biophysics@net.bio.net Sun Dec 15 22:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!www.nntp.primenet.com!nntp.primenet.com!uwm.edu!news-peer.gsl.net!news.gsl.net!news-penn.gsl.net!news.gsl.net!news.NetVision.net.il!news From: George Mallory Newsgroups: bionet.agroforestry,bionet.biophysics Subject: FREE pH measurement booklet Date: 16 Dec 1996 19:51:04 GMT Organization: NetVision LTD. Lines: 9 Message-ID: <594978$a1p@news.NetVision.net.il> NNTP-Posting-Host: ts010p3.pop2a.netvision.net.il Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) Xref: biosci bionet.agroforestry:4388 bionet.biophysics:2489 A free pH booklet is available which contains valuable information on basic pH measurement theory, pH measurement techniques, selecting the proper pH electrode for a particular application, and a pH troubleshooting guide. The booklet is available from Lazar Research Labs. Inc. by emailing service@lazarlab.com or faxing 1-213-931-1434. The booklet can also be obtained from the Lazar web site at http://www.lazarlab.com From owner-biophysics@net.bio.net Sun Dec 15 22:00:00 1996 Path: biosci!BIOSCI.MBP.MISSOURI.EDU!baskin From: baskin@BIOSCI.MBP.MISSOURI.EDU (Tobias Baskin) Newsgroups: bionet.biophysics Subject: data analysis/spline fit program Date: 15 Dec 1996 18:44:31 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Greetings, Anyone out there know of good software for fitting splines to data? At present, we are struggling with SAS, which works, but from which it is tricky (!) to extract the relevant coefficients. Note, we need to fit a smooth curve through data for which we do not have a model function, hence our interest in splines. We don't just want to drive a spline curve through each data point, which is all that a couple of programs we have checked can do. I realize that this is not explitly a biophysics or microscopy question, but as the our data have been generated from image analysis through a microscope, I hope I may be forgiven for supposing someone on these lists could help. Thanks, Tobias Baskin - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ___ ____ ^ ____ _____ Tobias I. Baskin / \ / / \ / \ / 1623 University Ave. / | / / \ / / Columbia, Missouri /___ / /__ /_____\ / /__ 65201 USA / / / \ ( / / / / \ \ / voice: 573 - 443-1984 / /____ / \ \____/ /_____ fax: 573 - 882-0123 From owner-biophysics@net.bio.net Sun Dec 15 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!www.nntp.primenet.com!nntp.primenet.com!uwm.edu!news-peer.gsl.net!news.gsl.net!news-penn.gsl.net!news.gsl.net!news.NetVision.net.il!news From: George Mallory Newsgroups: bionet.agroforestry,bionet.biophysics Subject: FREE pH measurement booklet Date: 16 Dec 1996 19:54:32 GMT Organization: NetVision LTD. Lines: 9 Message-ID: <5949do$a77@news.NetVision.net.il> NNTP-Posting-Host: ts010p3.pop2a.netvision.net.il Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) Xref: biosci bionet.agroforestry:4390 bionet.biophysics:2491 A free pH booklet is available which contains valuable information on basic pH measurement theory, pH measurement techniques, selecting the proper pH electrode for a particular application, and a pH troubleshooting guide. The booklet is available from Lazar Research Labs. Inc. by emailing service@lazarlab.com or faxing 1-213-931-1434. The booklet can also be obtained from the Lazar web site at http://www.lazarlab.com From owner-biophysics@net.bio.net Sun Dec 15 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!www.nntp.primenet.com!nntp.primenet.com!uwm.edu!news-peer.gsl.net!news.gsl.net!news-penn.gsl.net!news.gsl.net!news.NetVision.net.il!news From: George Mallory Newsgroups: bionet.agroforestry,bionet.biophysics Subject: FREE pH measurement booklet Date: 16 Dec 1996 19:51:47 GMT Organization: NetVision LTD. Lines: 9 Message-ID: <59498j$a63@news.NetVision.net.il> NNTP-Posting-Host: ts010p3.pop2a.netvision.net.il Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) Xref: biosci bionet.agroforestry:4389 bionet.biophysics:2490 A free pH booklet is available which contains valuable information on basic pH measurement theory, pH measurement techniques, selecting the proper pH electrode for a particular application, and a pH troubleshooting guide. The booklet is available from Lazar Research Labs. Inc. by emailing service@lazarlab.com or faxing 1-213-931-1434. The booklet can also be obtained from the Lazar web site at http://www.lazarlab.com From owner-biophysics@net.bio.net Mon Dec 16 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!www.nntp.primenet.com!nntp.primenet.com!EU.net!Austria.EU.net!01-newsfeed.univie.ac.at!swidir.switch.ch!serra.unipi.it!sirio.cineca.it!gopher From: Albertino Bigiani Newsgroups: bionet.biophysics Subject: biophysics Date: Tue, 17 Dec 1996 23:12:48 +0100 Organization: University of Modena Lines: 3 Message-ID: <32B71AE0.6340@c220.unimo.it> NNTP-Posting-Host: slip-12.unimo.it Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Macintosh; I; 68K) What is biophysics anyway? Albertino Bigiani From owner-biophysics@net.bio.net Mon Dec 16 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.biophysics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Dec 1996 02:00:48 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199612171000.CAA23528@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-biophysics@net.bio.net Tue Dec 17 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!www.nntp.primenet.com!nntp.primenet.com!mr.net!news.clark.net!usenet From: Mark Simmons Newsgroups: bionet.biophysics Subject: Find your research equipment online! Date: Tue, 17 Dec 1996 19:11:49 -0500 Organization: Johns Hopkins University Lines: 7 Message-ID: <32B736C5.129D@skyline.net> Reply-To: msimmons6@skyline.net NNTP-Posting-Host: athens.skyline.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Win95; I) Becton Dickinson Primary Care diagnostics, a manufacturer of high quality laboratory equipment, has recently published a major portion of their catalog on the Web. Products include: centrifuges, DNA Probes, Dry Hematology Systems. Visit them on the Web at http://www.bdpcd.com/ From owner-biophysics@net.bio.net Wed Dec 18 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.sgi.com!cgl!itssrv1.ucsf.edu!dek From: dek@socrates.ucsf.edu (David Konerding) Newsgroups: bionet.biophysics Subject: Re: biophysics Date: 19 Dec 1996 05:02:08 GMT Organization: UCSF, ITS Lines: 30 Message-ID: References: <32B71AE0.6340@c220.unimo.it> NNTP-Posting-Host: socrates.ucsf.edu X-Newsreader: slrn (0.9.1.1 BETA UNIX) In article <32B71AE0.6340@c220.unimo.it>, Albertino Bigiani wrote: >What is biophysics anyway? > >Albertino Bigiani A discipline and a methodology concerned with the physical properties of biological systems. In particular, the measurement and prediction of physical properties of macromolecules, cells, and organisms, through the use of many techniques not limited to: structural biology (xray crystallography, NMR, EPR, CD, UV) cellular biology (fluorescent cell sorting, optical and electron microscopy) radiation biology (xray) I find biophysics places a heavy emphasis on physical techniques which would be familiar to the physicist, and that statistics and chemistry play very important roles as well. -- -------------------------------------------------------------------------------- Email: dek@cgl.ucsf.edu David Konerding WWW: http://picasso.ucsf.edu/~dek -------------------------------------------------------------------------------- Snail: Graduate Group in Biophysics Medical Sciences 926, Box 0446 University of California San Francisco, CA 94143 From owner-biophysics@net.bio.net Wed Dec 18 22:00:00 1996 Path: biosci!agate!howland.erols.net!news-peer.gsl.net!news.gsl.net!news-dc.gsl.net!news.gsl.net!news.NetVision.net.il!news From: Steve Malin Newsgroups: bionet.cellbiol,bionet.cellbiol.cytonet,bionet.biophysics Subject: FREE booklet on measuring pH, ions, O2 in very SMALL SAMPLES Date: 19 Dec 1996 22:06:19 GMT Organization: NetVision LTD. Lines: 10 Message-ID: <59ce8r$d0i@news.NetVision.net.il> NNTP-Posting-Host: ts000p10.pop2a.netvision.net.il Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) Xref: biosci bionet.cellbiol:6249 bionet.cellbiol.cytonet:745 bionet.biophysics:2498 A free booklet is available which describes techniques for measuring pH, ions, and dissolved oxygen in very small samples down to 20 microliters. Sample vessels which can be routinely measured included 96 well plates, microcentrifuge tubes, NMR tubes, serum cups, and capillary tubes. Ions which can be measured include chloride, sodium, potassium, and cyanide. All probes require only a pH meter for measurement. For further details contact Lazar Research Labs. Inc. at service@lazarlab.com or see the Lazar web site at http://www.lazarlab.com From owner-biophysics@net.bio.net Fri Dec 20 22:00:00 1996 Path: biosci!CS.Arizona.EDU!news.Arizona.EDU!hamblin.math.byu.edu!sol.ctr.columbia.edu!news.msfc.nasa.gov!www.nntp.primenet.com!nntp.primenet.com!uwm.edu!news.cse.psu.edu!news.cc.swarthmore.edu!netnews.upenn.edu!taurus.fccc.edu!sauder From: sauder@castor.fccc.edu (John Michael Sauder) Newsgroups: bionet.biophysics Subject: Re: THE SIN AGAINST THE HOLY GHOST Date: 20 Dec 1996 15:24:09 GMT Organization: Fox Chase Cancer Center, Philadelphia, PA Lines: 10 Message-ID: <59eb2p$8dc@taurus.fccc.edu> References: <2.2.16.19961216014512.260f4f3e@RUBENS.ITS.UNIMELB.EDU.AU> NNTP-Posting-Host: castor.rm.fccc.edu In article <2.2.16.19961216014512.260f4f3e@RUBENS.ITS.UNIMELB.EDU.AU> bjo@RUBENS.ITS.UNIMELB.EDU.AU ("Bernard O'Loughlin") writes: > [snip] >This article has been posted at the same time to dozens of mailing lists. >*bows out* Uh... Wrong newsgroup. -- -- Mike S. (M_Sauder@fccc.edu) http://www.fccc.edu/research/labs/roder/mike/ From owner-biophysics@net.bio.net Sun Dec 22 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!portc02.blue.aol.com!audrey01.news.aol.com!not-for-mail From: sntnews@aol.com (Sntnews) Newsgroups: bionet.biophysics Subject: biophysicist wanted Date: 23 Dec 1996 23:27:13 GMT Organization: AOL http://www.aol.com Lines: 14 Message-ID: <19961223232600.SAA23776@ladder01.news.aol.com> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com HIGHER EDUCATION CONSULTANT B & J International, an aggressive and rapid growing company specializing in product development and technology transfer, has huge number of openings for full-time or part-time employment answering survey and distributing information. Especially the part-time positions, because of developing new products and testing new system, we need the input from almost everyone with higher education. You will be working flexible hours at home, communicating with us by e-mail. Very competitive compensation. For consideration, please forward your very brief background information with your e-mail address to: sntnews@aol.com. E.O.E. From owner-biophysics@net.bio.net Sat Dec 28 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!news.bconnex.net!news2.insinc.net!roger.interlynx.net!usenet From: "Lars Thomsen" Newsgroups: bionet.biophysics Subject: New patch clamp analysis program for free ! Date: 29 Dec 1996 07:38:38 GMT Organization: 115 South Oval Lines: 26 Message-ID: <01bbf55a$9a481e60$39c032cc@boris.gate2.interlynx.net> NNTP-Posting-Host: ppp47-max1.interlynx.net X-Newsreader: Microsoft Internet News 4.70.1155 I have created a patch clamp analysis program that currently reads Axon files from Clampex, Fetchex or Axotape. (operationmode=3 and 5). I have used a lot of time on the graphics routines, since it is what I lacked most. However, the final version will include all the analysis that you have in PClamp + some of that you can find in Axograph for the Mac + some new things. And another thing that I have tried to avoid is menus. Program with menus are so slow to work with, because you have to unfold layers and more layers of submenus before you get to the point. I know what I want and that is a graph that I can use in my analysis and a figure that I can use as a figure. So I have tried to make it a very work friendly interface. Lots of the programs that I have seen until now simply is so bad if you e.g. have 100 files to analysis (and I have more - probably you too). You can get a preview of a little part of the program at http://home.interlynx.net/~lthomsen/hotspot.htm and if you are desparate then I am willing to give you a beta version, but then I think it is fair that you give me some feedback. -- Lars Thomsen, M.Sc. PhD. 115 South Oval, Hamilton, ON Canada L8S1R2 Email lthomsen@interlynx.net http://home.interlynx.net/~lthomsen/index.htm