From owner-biophysics@net.bio.net Wed Jan 01 22:00:00 1997 Path: biosci!PUBLIC.TPT.TJ.CN!yangwenx From: yangwenx@PUBLIC.TPT.TJ.CN (BIOPHYSICS DIVISION) Newsgroups: bionet.biophysics Subject: need help Date: 2 Jan 1997 04:51:29 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <199701021251.UAA12893@public.tpt.tj.cn> NNTP-Posting-Host: net.bio.net Hello, everybody.Happy new year. I am now preparing to give a lecture on cell biology for my students. I need some materials on cell signals transduction pathway. Where can I get the latest reports or progress and how to download them? Who knows? Please tell me. Thanks in advance. Thanks again. Yang Wen xiu From owner-biophysics@net.bio.net Wed Jan 01 22:00:00 1997 Path: biosci!rutgers!gatech!www.nntp.primenet.com!nntp.primenet.com!dciteleport.com!feed1.news.erols.com!news-xfer.netaxs.com!newsfeeds.sol.net!uniserve!van-bc!unixg.ubc.ca!news.bc.net!torn!resunix.sickkids.on.ca!NewsWatcher!user From: Newswatcher@sickkids.on.ca (Newswatcher) Newsgroups: bionet.biophysics Subject: Room Sharing at Biopyhsical Society Meeting Date: Thu, 02 Jan 1997 15:46:39 -0600 Organization: The Hospital for Sick Children Lines: 19 Message-ID: NNTP-Posting-Host: 142.20.24.101 Hi, Friends: I am looking for a female nonsmoker to share a twin room (Holiday Inn Superdome) during the Biophysical Society Meeting, March 1-6, 1997 in New Orleans, Louisiana. If you're interested, would you please contact me at the following address? I am a quite female, nonsmoker. My arrival date is March 1st, departure on March 6. I would be happy to hear from you. My phone number is 416-813-5855, fax number is 416-813-5022, Atten: Li-Ping. E-Mail: lliu@sickkids.on.ca Best Regards Li-Ping Liu Biochem. Res., Room 3523 Hospital for Sick Children Toronto, Canada M5G 1X8 -- Do not send email to Newswatcher@sickkids.on.ca. It will be bounced. From owner-biophysics@net.bio.net Thu Jan 02 22:00:00 1997 Path: biosci!RAINY.CEMS.UMN.EDU!acre From: acre@RAINY.CEMS.UMN.EDU (acre) Newsgroups: bionet.biophysics Subject: Search Announcement Date: 3 Jan 1997 14:37:28 -0800 Organization: Unversity of Minnesota Lines: 45 Sender: daemon@net.bio.net Distribution: world Message-ID: <32CD87B4.502F@rainy.cems.umn.edu> NNTP-Posting-Host: net.bio.net DIRECTOR - BIOMEDICAL ENGINEERING INSTITUTE UNIVERSITY OF MINNESOTA The University of Minnesota seeks a Director for its recently established Biomedical Engineering Institute (BMEI). BMEI is the administrative home of the graduate degree program in Biomedical Engineering and is a cooperative enterprise coordinating the biomedical engineering activities of the Medical School and the Institute of Technology (Minnesota's combined college of mathematics, physical science and engineering). The Director will report to the Deans of these two colleges. The Director will hold an endowed Bakken chair professorship. BMEI has active research and industrial interaction programs, in addition to its responsibility for graduate education. The graduate program (which was initiated in 1972) has fifty full-time and twenty part-time students, the latter group being drawn largely from the exceptionally broad range of biomedical industries in the region. BMEI is supported by funds from the recurring operating budget of the University of Minnesota, and will be the recipient of the income from an on-going, $12 million, endowment campaign which is well on its way to completion. The Director should have a recognized reputation for excellence in research accomplishments and the ability to lead and administer a growing academic enterprise. It is planned that BMEI will grow in staff, both via new faculty hiring and by the transfer of appointments of existing faculty into BMEI. The Director will be expected to lead the development of BMEI into an internationally recognized program of biomedical engineering research, education and industrial interaction. Interested candidates should send a resume by January 15, 1997 to Professor Wei-Shou Hu Department of Chemical Engineering and Materials Science University of Minnesota 421 Washington Avenue SE Minneapolis, MN 55455 phone: 612-625-0546 fax: 612-626-7246 e-mail: wshu@cems.umn.edu Please contact Dr. Hu at the above address with questions or nominations, or visit the Biomedical Engineering Institute home page at http://www.med.umn.edu/bmei/. The University of Minnesota is an equal opportunity educator and employer. From owner-biophysics@net.bio.net Fri Jan 03 22:00:00 1997 Path: biosci!agate!howland.erols.net!feed1.news.erols.com!news.bconnex.net!news2.insinc.net!roger.interlynx.net!usenet From: "Lars Thomsen" Newsgroups: bionet.biophysics Subject: PatchReader beta version 0.1 ready on sunday 1200 noon Date: 4 Jan 1997 07:56:27 GMT Organization: 115 South Oval Lines: 24 Message-ID: <01bbfa14$07362720$4cc032cc@boris.gate2.interlynx.net> NNTP-Posting-Host: ppp16-max2.interlynx.net X-Newsreader: Microsoft Internet News 4.70.1155 The first release of a preliminary version of my patch clamp analysis program is ready for download from my web site sunday 1200 noon. Take a preview at some of the programs current capabilities at : http://home.interlynx.net/~lthomsen/hotspot.htm The program has highly professional graphical routines. The program is not commercial or shareware, it is simply a help from me to you. The analysis is not so advanced yet, but Im working hard on fixing that. Right now the program is a really nice alternative to your PClamp6 graph struggling. If you want to become a betatester and thereby having some influence on the future shape of the program then you can download it at Sunday. However, you need a password to the zip file. You can get the password by sending me an email. lthomsen@interlynx.net See you -- Lars Thomsen, M.Sc. PhD. 115 South Oval, Hamilton, ON Canada L8S1R2 Email lthomsen@interlynx.net http://home.interlynx.net/~lthomsen/index.htm From owner-biophysics@net.bio.net Sat Jan 04 22:00:00 1997 Path: biosci!agate!howland.erols.net!newsfeed.internetmci.com!news.corpcomm.net!maggie.ionsys.com!news.rns.net!news2.compulink.com!news3.idirect.com!island.idirect.com!discordnet1.idirect.com Newsgroups: bionet.biophysics From: trevor@idirect.com (Trevor Pedley) Subject: Action Potential and ECG X-Newsreader: Forte Agent .99e/32.227 X-Newsmaster: Laszlo Herczeg (las@idirect.com) Lines: 14 X-Client-Port: 1148 Message-ID: <32cb3330.2620608@news2.compulink.com> X-Nntp-Posting-Host: island.idirect.com Date: 2 Jan 97 01:26:55 UTC Lines: 14 hello! I am trying to understand the relationship of the changes in the action potential on the ECG.; in particular the changes in Phase 2 and 3 and how if they are shortened or increased do they alter the effect on the ECG? For example, does shortening phase 2 d/t say ischemia, decrease the amplitude of the ECG? What happens if these phases (2;3) are increased or lengthened to the ECG? If there is anyone who has extensive knowledge in this area and would be willing to answer these questions and a few others, it would be greatly appreciated. Emailing me may make this process of communication a little more speedy, so if you would like, send your info to trevor@worldy.com If there is anyway Ican help you out, please pass it on and I would be willing to try! thanks Trevor From owner-biophysics@net.bio.net Sun Jan 05 22:00:00 1997 Path: biosci!citi2.fr!bali From: bali@citi2.fr (Moez BALI) Newsgroups: bionet.biophysics Subject: test Date: 6 Jan 1997 11:10:03 -0800 Organization: INSERM U.426 Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <32D14E97.7C99@bisance.citi2.fr> Reply-To: bali@citi2.fr NNTP-Posting-Host: net.bio.net just a test From owner-biophysics@net.bio.net Tue Jan 07 22:00:00 1997 Path: biosci!GRIFFON.MWSC.EDU!hrk7027 From: hrk7027@GRIFFON.MWSC.EDU (Henoch Kristianto) Newsgroups: bionet.biophysics Subject: Re: PLEASE RESPOND EVERYONE PRIVATELY TO STEALTH@ICAN.NET Date: 8 Jan 1997 08:34:27 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 133 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Get screwed! That is suck! On Tue, 7 Jan 1997, M Dunlop wrote: > > Hi there!!!!! I'm looking for a broad range of people to > answer this survey and e-mail this filled out form to: > > stealth@ican.net > > This survey is to help me complete my thesis for my > Honours Bachelor of Science in Forestry. > > When answering the yes/no questions please feel free to add your > own comments. When answering the multiple choice please (X) the > appropriate answer. Please answer the questions to the best of > your knowledge. > > If you have any questions or further comments please feel free to > e-mail me at the address above. > > 1. Where do you live? (Province and City) > > 2. What do you do for a living? > > 3. How old are you? > > 4. What was the last grade that you completed? > > 5. What is your definition of a forest > ( )a) a source of timber > ( )b) a source of food > ( )c) a place for recreation > ( )d) habitat for plants and animals > ( )e) a source of economic gain > ( )f) other please specify > > 6. Do you use the forest for anything? (yes/no) > > 6b. If yes, please list what you use the forest for (hunting, > fishing, hiking etc.). > > 7. What is the greatest threat to the forest > ( )a) logging > ( )b) pollution > ( )c) forest fires and insects > ( )d) human intervention in nature > ( )e) other please specify > > 8. Do you feel we are degrading the forest? (yes/no) > > 9. Do you feel we are protecting the forest? (yes/no) > > 10. Do you feel that we should be using the forest for economic > gain? (yes/no) > > 11. Do you think we are planting enough trees? (yes/no) > > 12. Do you think the forest industry or the government should be > responsible for planting trees (reforestation)? (yes/no) > > 13. What would you consider good reforestation practices > ( )a) planting the same species of trees > ( )b) planting different species of trees in the same > area > ( )c) allowing the forest to return naturally > > > 14. Do you think the forest industry needs regulating by the > government? (yes/no) > > 15. If yes, why? > > 16. Should we be managing our forests? (yes/no) > > 17. If yes, what do you consider good management policies > ( )a) managing for economic gain > ( )b) managing for recreation i.e. fishing, hunting > ( )c) managing for a diverse forest i.e. different > plants and animals > ( )d) managing for conservation of the forest and it's > resources > ( )e) other please specify > > 18. Do you think the forest industry is doing enough to protect > the environment? (yes/no) > > 19. Is there any harvesting/logging in your area? (yes/no) > > 20. Is the forest industry harvesting (cutting) too many trees, > just enough trees, or too few trees? > > 21. Do you think clearcutting is a good forest practice? > (yes/no) > > 22. Do you think clearcuts are > ( )a) too widely used > ( )b) used just enough > ( )c) not used enough > > 23. Should we allow harvesting in old growth forests? (yes/no) > > 24. If yes, who should regulate the amount cut > ( )a) the forest industry > ( )b) the government > ( )c) the public > > 25. Do you think there are too many logging roads? (yes/no) > > 26. Do you think the logging roads give us too much access to > the forest? (yes/no) > > 27. If yes or no please explain. > > 28. If an educational program on forestry practices and the > forest industry were to become available in your area would you > attend? (yes/no) > A) if yes, why? > B) if no, why not? > > 29. Would you become involved with the forest industry if public > involvement was encouraged? (yes/no) > A) if yes, why? > > B) if no, why not? > > 30. If you could attend a course/seminar on forestry what would > you like to see covered? > > THANK YOU FOR YOUR TIME. If you know any one else who wouldn't > mind taking the time to fill out this survey I would really > appreciate their input.. > From owner-biophysics@net.bio.net Tue Jan 07 22:00:00 1997 Path: biosci!ihnp4.ucsd.edu!swrinde!howland.erols.net!newsfeed.internetmci.com!uunet!in3.uu.net!136.142.185.26!newsfeed.pitt.edu!bb3.andrew.cmu.edu!andrew.cmu.edu!dp2e+ From: Doros T Petasis Newsgroups: bionet.biophysics Subject: test Date: Wed, 8 Jan 1997 12:19:13 -0500 Organization: Chemistry, Carnegie Mellon, Pittsburgh, PA Lines: 1 Message-ID: NNTP-Posting-Host: po9.andrew.cmu.edu test post From owner-biophysics@net.bio.net Wed Jan 08 22:00:00 1997 Path: biosci!rutgers!gatech!csulb.edu!hammer.uoregon.edu!news-peer.gsl.net!news.gsl.net!news-penn.gsl.net!news.gsl.net!news.NetVision.net.il!news From: Mark Klein Newsgroups: bionet.cellbiol,bionet.biophysics Subject: FREE pH measurement booklet Date: 9 Jan 1997 10:54:24 GMT Organization: NetVision LTD. Lines: 9 Message-ID: <5b2ip0$2bp@news.NetVision.net.il> NNTP-Posting-Host: ts006p13.pop2a.netvision.net.il Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) Xref: biosci bionet.cellbiol:6377 bionet.biophysics:2525 A free pH booklet is available which contains valuable information on basic pH measurement theory, pH measurement techniques, selecting the proper pH electode for a particular application, and a pH troubleshooting guide. The booklet is available from Lazar Research Labs. Inc. by emailing service@lazarlab.com or faxing 1-213-931-1434. The booklet can also be obtained from the Lazar web site at http://www.lazarlab.com From owner-biophysics@net.bio.net Wed Jan 08 22:00:00 1997 Path: biosci!daresbury!not-for-mail From: Erik De Schutter Newsgroups: bionet.biophysics Subject: Crete Course in Computational Neuroscience Date: 9 Jan 1997 15:25:57 -0000 Lines: 94 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5b32m5$lsn@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk FIRST CALL CRETE COURSE IN COMPUTATIONAL NEUROSCIENCE SEPTEMBER 7 - OCTOBER 3, 1997 UNIVERSITY OF CRETE, GREECE DIRECTORS: Erik De Schutter (University of Antwerp, Belgium) Idan Segev (Hebrew University, Jerusalem, Israel) Jim Bower (California Institute of Technology, USA) Adonis Moschovakis (University of Crete, Greece) The Crete Course in Computational Neuroscience introduces students to the practical application of computational methods in neuroscience, in particular how to create biologically realistic models of neurons and networks. The course consists of two complimentary parts. A distinguished international faculty gives morning lectures on topics in experimental and computational neuroscience. The rest of the day is spent learning how to use simulation software and how to implement a model of the system the student wishes to study. The first week of the course introduces students to the most important techniques in modeling single cells, networks and neural systems. Students learn how to use the GENESIS, NEURON, XPP and other software packages on their individual unix workstations. During the following three weeks the lectures will be more general, but each week topics ranging from modeling single cells and subcellular processes through the simulation of simple circuits, large neuronal networks and system level models of the the brain will be covered. The course ends with a presentation of the students' modeling projects. The Crete Course in Computational Neuroscience is designed for advanced graduate students and postdoctoral fellows in a variety of disciplines, including neuroscience, physics, electrical engineering, computer science and psychology. Students are expected to have a basic background in neurobiology as well as some computer experience. A total of 28 students will be accepted with an age limit of 35 years. We will accept students of any nationality, but the majority will be from the European Union and affiliated countries (Iceland, Israel, Liechtenstein and Norway). We specifically encourage applications from researchers who work in less-favoured regions of the EU, from women and from researchers from industry. Every student will be charged a tuition fee of 500 ECU (approx. US$630). In the case of students with a nationality from the EU, affiliated countries or Japan, the tuition fee covers lodging, local travel and all course-related expenses. All applicants with other nationalities will be charged an ADDITIONAL fee of 1000 ECU (approx. US$1260) which covers lodging, local travel and course-related expenses. For nationals from EU and affiliated countries economy travel from an EU country to Crete will be refunded after the course. A limited number of students from less-favoured regions world-wide will get their fees and travel refunded. More information and application forms can be obtained: - WWW access: http://bbf-www.uia.ac.be/Crete_index.html Please apply electronically using a web browser if possible. - email: crete_course@bbf.uia.ac.be - by mail: Prof. E. De Schutter Born-Bunge Foundation University of Antwerp - UIA, Universiteitsplein 1 B2610 Antwerp Belgium FAX: +32-3-8202669 APPLICATION DEADLINE: April 5, 1996. Applicants will be notified of the results of the selection procedures by May 5. FACULTY: L. Abbott (Brandeis University, USA), D. Beeman (University of Colorado, Boulder, USA), A. Borst (Max Planck Institute Tuebingen, Germany), R. Calabrese (Emory University, USA), A. Destexhe (Universite Laval, Canada), M. Hines (Yale University, USA), J.J.B. Jack (Oxford University, England), C. Koch (California Institute of Technology, USA), R. Kotter (Heinrich Heine University Dusseldorf, Germany), G. LeMasson (University of Bordeaux, France), K. Martin (Institute of Neuroinformatics, Zurich), M. Nicolelis (Duke University, USA), S. Redman (Australia National University Canberra), J.M. Rinzel (NIH, USA), S.A. Shamma (University of Maryland, USA), H. Sompolinsky (Hebrew University Jerusalem, Israel), S. Tanaka (RIKEN, Japan), A.M. Thomson (Royal Free Hospital, England), T.L. Williams (St George Hospital, London, England), Y. Yarom (Hebrew University Jerusalem, Israel), and others to be named. The Crete Course in Computational Neuroscience is supported by the European Commission (4th Framework Training and Mobility of Researchers program), by The Brain Science Foundation (Tokyo) and by UNESCO. Local administrative organization: the Institute of Applied and Computational Mathematics of FORTH (Crete, GR). From owner-biophysics@net.bio.net Wed Jan 08 22:00:00 1997 Path: biosci!agate!spool.mu.edu!howland.erols.net!math.ohio-state.edu!jussieu.fr!wheeler From: wheeler@lpbc.jussieu.fr (Gavin Wheeler) Newsgroups: bionet.biophysics,sci.bio.misc Subject: C-Form DNA in solution? Date: Fri, 10 Jan 1997 04:13:03 +0100 Organization: Poor Lines: 15 Message-ID: NNTP-Posting-Host: lpbcmifluo2.lpbc.jussieu.fr Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Yet Another NewsWatcher 2.3.2 Xref: biosci bionet.biophysics:2526 sci.bio.misc:6909 Greetings! We're (or rather, my PhD supervisor is..) looking for the conditions necessary to observe the C-form of DNA in solution. So far, all we have found in the literature are the conditions necessary for the C-form in solid fibres, but da boss swears he's seen an article on the C-form of DNA in solution *somewhere*. So, does anyone out there know of a handy reference, or do they have the conditions (what concentration of what ions etc ) ? Please? Thanks in advance for any help you can give. Email would probably be the best medium for a reply, unless others show an interest on this group. -- Gavin Wheeler wheeler@lpbc.jussieu.fr From owner-biophysics@net.bio.net Wed Jan 08 22:00:00 1997 Path: biosci!om.cv.hp.com!jim_cooper From: jim_cooper@om.cv.hp.com Newsgroups: bionet.biophysics Subject: Re: PLEASE RESPOND EVERYONE PRIVATELY TO STEALTH@ICAN.NET Date: 9 Jan 1997 14:31:02 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 288 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Item Subject: PLEASE RESPOND EVERYONE PRIVATELY TO STEALTH@ICAN.NET Received: from hp-cv.cv.hp.com by hp-pcd.cv.hp.com with ESMTP (1.37.109.16/15.5+IOS 3.22+OM+CV 1.0) id AA027855795; Thu, 9 Jan 1997 13:36:35 -0800 Received: from www19.w3.org (www19.w3.org [18.29.0.19]) by hp-cv.cv.hp.com (8.8.4/8.8.0) with SMTP id NAA02731 for ; Thu, 9 Jan 1997 13:36:30 -0800 (PST) Received: by www19.w3.org (8.6.12/8.6.12) id SAA08784; Tue, 7 Jan 1997 18:43:34 -0500 Resent-Date: Tue, 7 Jan 1997 18:43:34 -0500 Resent-Message-Id: <199701072343.SAA08784@www19.w3.org> Message-Id: Comments: Authenticated sender is From: www-talk-request@www10.w3.org To: 3COM-L%NUSVM.BITNET@mitvma.mit.edu, 3D@lbl.gov, 3DBLASTER@CREATIVE-LABS.CO.UK, 78-L@CORNELLC.CIT.CORNELL.EDU, 90210@ferkel.ucsb.edu, 9370-L%HEARN.BITNET@mitvma.mit.edu, A.RICE%HAMPVMS.BITNET@VM1.NODAK.EDU, AACRL@UABDPO.DPO.UAB.EDU, AAI@ST-LOUIS-EMH2.ARMY.MIL, AAVLD-L@LISTSERV.NET, ACADV@VM1.NODAK.EDU, ACCENTSERVER@NIS.COM, ACCESS-L@eva.dc.LSOFT.COM, ACCORDION@CS.CMU.EDU, ACCRI-L@UABDPO.DPO.UAB.EDU, ACROBAT@BLUEWORLD.COM, ACSOFT-L@WUVMD.WUSTL.EDU, ACT-UP@WORLD.STD.COM, ACTNOW-L@BROWNVM.BROWN.EDU, ADD-PARENTS@MV.MV.COM, ADDICT-L@KENTVM.KENT.EDU, ADND-L%UTARLVM1.BITNET@mitvma.mit.edu, ADOPTEES@UCSD.EDU, ADVANC-L@LISTSERV.NET, ADVISE-L%CANADA01.BITNET@mitvma.mit.edu, AE@SJSUVM1.SJSU.EDU, AECM-L@LOYOLA.EDU, AECUDEP-L@ADMIN.ACES.K12.CT.US, AERONAUTICS@rascal.ics.utexas.edu, AEROSP-L@SIVM.SI.EDU, AFAS-L@KENTVM.KENT.EDU, AFRICA-L@oraculo.lcc.ufmg.br, AFRICA-L@VTVM1.CC.VT.EDU, AFRICANA@wmvm1.cc.wm.edu, AG-EXP-L@VM1.NODAK.EDU, AI-CHI@LLL-LCC.LLNL.GOV, AI-ED@SUN.COM, AI-KAPPA-PC@mailbase.ac.uk, AI-MEDICINE@MED.STANFORD.EDU, AIBI-L@ACADVM1.UOTTAWA.CA, AIDS@CS.UCLA.EDU, AIKIDO-L@PSUVM.PSU.EDU, AIL-L@austin.onu.edu, AIRLINE@CUNYVM.CUNY.EDU, AIRPLANE-CLUBS@DG-RTP.DG.COM, AIUG@DEAKIN.EDU.AU, AIX-L%BUACCA.BITNET@mitvma.mit.edu, AIXNEWS@PUCC.PRINCETON.EDU, AJAX@XS4ALL.nl, AJBS-L@PUCC.PRINCETON.EDU, ALA-PLAN@SUN.CC.WESTGA.EDU, ALCOHOL%LMUACAD.BITNET@mitvma.mit.edu, ALCOHOL-PSYCHOL@mailbase.ac.uk, ALICEFAN%WKUVX1.BITNET@mitvma.mit.edu, ALL-OF-ELSA@jus.uio.no, ALLERGY@TAMVM1.TAMU.EDU, ALLIN1-L@CCVM.SUNYSB.EDU, ALLMUSIC@AMERICAN.EDU, ALM-NEWS@ESUSDA.GOV, ALPHA-OSF-MANAGERS@ORNL.GOV, ALT-PHOTO-PROCESS@VAST.UNSW.EDU.AU, ALZHEIMER@WUBIOS.WUSTL.EDU, AMALGAM@gmd.de, AMART-L@CUNYVM.CUNY.EDU, AMERCATH@UKCC.UKY.EDU, AMFM@TQMCOMMS.CO.UK, AMIA-L@UKCC.UKY.EDU, AMIGA@varano.ing.unico.it, AMIGA-M2@VIRGINIA.EDU, AMLIT-L@mizzou1.missouri.edu, AMNESTY@VMS.CIS.PITT.EDU, AMP-L@VM.GMD.DE, AMSSIS-L@uafsysb.uark.edu, ANALOGUE@MAGNUS.ACS.OHIO-STATE.EDU, ANCIEN-L@ULKYVM.LOUISVILLE.EDU, ANDREW-DEMOS@ANDREW.CMU.EDU, ANEST-L@UBVM.CC.BUFFALO.EDU, ANIMAL-RIGHTS@CS.ODU.EDU, ANMI-L@RMCS.CRANFIELD.AC.UK, ANN-LOTS@VM1.NODAK.EDU, ANNEAL@CS.UCLA.EDU, ANSAXNET@WVNVM.WVNET.EDU, ANTHRO-L%UBVM.BITNET@mitvma.mit.edu, ANU-NEWS@VM1.NODAK.EDU, AON@polyslo.csc.calpoly.edu, APE-INFO@ferkel.ucsb.edu, APL-L%UNB.CA@VM1.NODAK.EDU, APO-L@VM.CC.PURDUE.EDU, APOGEES%FRAIX11.BITNET@VM1.NODAK.EDU, APOLLO-L%UMRVMB.BITNET@VM1.NODAK.EDU, APOLLO@UMIX.CC.UMICH.EDU, APOLOGIA-L@netcom.com, APOSOC-L@PSUVM.PSU.EDU, APPC-L@AMERICAN.EDU, APPLE-CROP-MG@ESUSDA.GOV, APPLIT@MSUACAD.MOREHEAD-ST.EDU, AQUA-L@VM.UOGUELPH.CA, AQUARIUM@EMUVM1.CC.EMORY.EDU, AR@aol.COM, AR-Alerts@ny.neavs.com, ARAB-AMERICAN@CARLETON.EDU, ARCANA@BROWNVM.BROWN.EDU, ARCH-L@gwdu42.gwdg.de, ARCH-THEORY@mailbase.ac.uk, 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BONSAI@CMS.CC.WAYNE.EDU, BOOK-TALK@COLUMBIA.ILC.COM, BOOKNEWS@COLUMBIA.ILC.COM, BOSOX-DIGEST@WORLD.STD.COM, BOSOX@WORLD.STD.COM, BOSTON-BOOK@WORLD.STD.COM, BOSTON-RSI@WORLD.STD.COM, BPM@ANDREW.CMU.EDU, BRAS-NET@CS.COLUMBIA.EDU, BRASS@quartz.gly.fsu.edu, BREAD-BAKERS-DIGEST@BEST.COM, BREAST-CANCER@MORGAN.UCS.MUN.CA, BRITISH-CARS@AUTOX.TEAM.NET, BROM-L@FTPT.BR, BRONTE@WORLD.STD.COM, BROOMS-L@TREARNPC.EGE.EDU.TR, BRS-L%uscvm.bitnet@VM1.NODAK.EDU, BRTHPRNT@INDYCMS.IUPUI.EDU, BRUNONIA@BROWNVM.BROWN.EDU, BTL@amnesiac.NMSU.Edu, BUBBA-L@KNUTH.MTSU.EDU, BUDDHA-L@ULKYVM.LOUISVILLE.EDU, BUDDHIST-PHILOSOPHY@THINK.NET, BUDGET-NET@FINANCENET.GOV, BUGNET@WSUVM1.CSC.WSU.EDU, BUILT-ENVIRONMENT@mailbase.ac.uk, BULLDOGX-L@io.com, BURG-CEN@NIC.SURFNET.NL, BUSCOM-L@UTEPVM.UTEP.EDU, BUSFAC-L@CMUVM.CSV.CMICH.EDU, C-NEWS@WORLD.STD.COM, C14-L@LISTSERV.ARIZONA.EDU, C18-STUDIES@mailbase.ac.uk, CANLIT-L@NLC-BNC.CA, CARP-L@XS4ALL.nl, CAT-CHAT@UKCC.UKY.EDU, CATHOLIC@SARTO.GAITHERSBURG.MD.US, CATHOLIC-ACTION@VPNET.CHI.IL.US, CAVERS-DIGEST@bambam.tacoma.washington.edu, CB-NET@BGU.EDU, CBA@GS1.COM, CBR-L@PO.CWRU.EDU, CCMI-L@USA.NET, CD-ROM@DAYTONSHAREWARE.COM, CD-ROM.Online@NSIMULTIMEDIA.COM, CDN-FIREARMS-ALERT@SFN.SASKATOON.SK.CA, CDN-FIREARMS-DIGEST@SFN.SASKATOON.SK.CA, CDN-NUCL-L@MCMASTER.CA, CDR-L@vm.tulsa.cc.ok.us, CDROMLAN@IDBSU.IDBSU.EDU, CELIAC@SJUVM.STJOHNS.EDU, CELLWALL@VM1.NODAK.EDU, CERT-ADVISORY@CERT.ORG, CET-MG@ESUSDA.GOV, CET-NEWS@ESUSDA.GOV, CFD@UKCC.UKY.EDU, CHANTER-LISTE@WIMSEY.COM, CHANTEUSE-LISTE@WIMSEY.CO Date: Tue, 7 Jan 1997 12:53:40 +0000 Subject: PLEASE RESPOND EVERYONE PRIVATELY TO STEALTH@ICAN.NET Reply-To: stealth@ican.net Priority: normal X-Mailer: Pegasus Mail for Windows (v2.23) X-List-Url: http://www.w3.org/pub/WWW/Archives/Public/www-talk/ Resent-From: www-talk@www10.w3.org X-Mailing-List: archive/latest/3157 X-Loop: www-talk@w3.org Sender: www-talk-request@www10.w3.org Resent-Sender: www-talk-request@www10.w3.org Precedence: list ....................................................................... Item Subject: PLEASE RESPOND EVERYONE PRIVATELY TO STEALTH@ICAN.NET Sorry, but I don't think so...... ______________________________ Reply Separator _________________________________ Subject: PLEASE RESPOND EVERYONE PRIVATELY TO STEALTH@ICAN.NET Author: Non-HP-www-talk-request (www-talk-request@www10.w3.org) at HP-Corvallis,shargw1 Date: 1/7/97 3:53 AM Hi there!!!!! I'm looking for a broad range of people to answer this survey and e-mail this filled out form to: stealth@ican.net This survey is to help me complete my thesis for my Honours Bachelor of Science in Forestry. When answering the yes/no questions please feel free to add your own comments. When answering the multiple choice please (X) the appropriate answer. Please answer the questions to the best of your knowledge. If you have any questions or further comments please feel free to e-mail me at the address above. 1. Where do you live? (Province and City) 2. What do you do for a living? 3. How old are you? 4. What was the last grade that you completed? 5. What is your definition of a forest ( )a) a source of timber ( )b) a source of food ( )c) a place for recreation ( )d) habitat for plants and animals ( )e) a source of economic gain ( )f) other please specify 6. Do you use the forest for anything? (yes/no) 6b. If yes, please list what you use the forest for (hunting, fishing, hiking etc.). 7. What is the greatest threat to the forest ( )a) logging ( )b) pollution ( )c) forest fires and insects ( )d) human intervention in nature ( )e) other please specify 8. Do you feel we are degrading the forest? (yes/no) 9. Do you feel we are protecting the forest? (yes/no) 10. Do you feel that we should be using the forest for economic gain? (yes/no) 11. Do you think we are planting enough trees? (yes/no) 12. Do you think the forest industry or the government should be responsible for planting trees (reforestation)? (yes/no) 13. What would you consider good reforestation practices ( )a) planting the same species of trees ( )b) planting different species of trees in the same area ( )c) allowing the forest to return naturally 14. Do you think the forest industry needs regulating by the government? (yes/no) 15. If yes, why? 16. Should we be managing our forests? (yes/no) 17. If yes, what do you consider good management policies ( )a) managing for economic gain ( )b) managing for recreation i.e. fishing, hunting ( )c) managing for a diverse forest i.e. different plants and animals ( )d) managing for conservation of the forest and it's resources ( )e) other please specify 18. Do you think the forest industry is doing enough to protect the environment? (yes/no) 19. Is there any harvesting/logging in your area? (yes/no) 20. Is the forest industry harvesting (cutting) too many trees, just enough trees, or too few trees? 21. Do you think clearcutting is a good forest practice? (yes/no) 22. Do you think clearcuts are ( )a) too widely used ( )b) used just enough ( )c) not used enough 23. Should we allow harvesting in old growth forests? (yes/no) 24. If yes, who should regulate the amount cut ( )a) the forest industry ( )b) the government ( )c) the public 25. Do you think there are too many logging roads? (yes/no) 26. Do you think the logging roads give us too much access to the forest? (yes/no) 27. If yes or no please explain. 28. If an educational program on forestry practices and the forest industry were to become available in your area would you attend? (yes/no) A) if yes, why? B) if no, why not? 29. Would you become involved with the forest industry if public involvement was encouraged? (yes/no) A) if yes, why? B) if no, why not? 30. If you could attend a course/seminar on forestry what would you like to see covered? THANK YOU FOR YOUR TIME. If you know any one else who wouldn't mind taking the time to fill out this survey I would really appreciate their input.. From owner-biophysics@net.bio.net Thu Jan 09 22:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news-xfer.netaxs.com!hammer.uoregon.edu!news.uoregon.edu!news.rediris.es!news.laeff.esa.es!not-for-mail From: Juan Carlos Gil Montoro Newsgroups: bionet.biophysics,sci.bio.misc Subject: Re: C-Form DNA in solution? Date: Fri, 10 Jan 1997 09:46:09 -0800 Organization: G.M.V. SA Lines: 27 Message-ID: <32D68061.758@gmv.es> References: NNTP-Posting-Host: pcsmt1.vilspa.esa.es Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Win16; I) Xref: biosci bionet.biophysics:2530 sci.bio.misc:6926 Gavin Wheeler wrote: > > Greetings! > > We're (or rather, my PhD supervisor is..) looking for the conditions > necessary to observe the C-form of DNA in solution. So far, all we have > found in the literature are the conditions necessary for the C-form in > solid fibres, but da boss swears he's seen an article on the C-form of DNA > in solution *somewhere*. So, does anyone out there know of a handy > reference, or do they have the conditions (what concentration of what ions > etc ) ? Please? > Hi there. Have a look at F.M. Pohl, "Polymorphism of a Synthetic DNA in Solution", Nature, vol. 260, 365 (1976). Pohl says the C-form of DNA is found at [CsCl]=7.1 M or [LiCl]=3.8 M. My Ph.D. is about the simulation of the B->Z DNA transition. Contact me if that fancies you. Regards. ==================================================================== Juan Carlos Gil Montoro G.M.V. SA mailto:jgil@gmv.es From owner-biophysics@net.bio.net Fri Jan 10 22:00:00 1997 Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!news.uoregon.edu!tezcat!feed1.news.erols.com!news.dra.com!news.good.net!news.good.net!news.goodnet.com!phx-ts2-16.goodnet.com!user From: mthompson@asu.edu (MThompson) Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Subject: What is the SMALLEST DNA BINDING DOMAIN? Date: Fri, 10 Jan 1997 13:11:27 -0700 Organization: Arizona State University Lines: 5 Message-ID: NNTP-Posting-Host: phx-ts2-16.goodnet.com Xref: biosci bionet.biophysics:2531 bionet.molbio.proteins:9694 bionet.info-theory:4517 bionet.molbio.evolution:5503 bionet.molbio.methds-reagnts:53365 I am searching for a small independently folding (or in presence of a metal ion), DNA binding domain. Would like it to be sequence specific, but would greatly appreciate all suggestions. Be as detailed as possible or please cite a reference, or name that I can locate it in the literature. Thanks very much. From owner-biophysics@net.bio.net Fri Jan 10 22:00:00 1997 Path: biosci!ucdavis.edu!smmusser From: smmusser@ucdavis.edu (Siegfried Musser) Newsgroups: bionet.biophysics Subject: Re: Looking for ideas for qualification exam proposal Date: 10 Jan 1997 20:18:42 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <32d6ebbe.3399508@news.sprynet.com> NNTP-Posting-Host: net.bio.net > I am a graduate student at the biochemistry program and this year I > will have to take my qualification exam . I am looking for interesting > ideas for the written qualification exam proposal. The proposal should > be similar to real grant proposal in structural organisation and can > cover any area of molecular biology, cellular bilology, biochemistry, > physiology etc. Topic for it should be chosen so that it would be easy > to come up with new research ideas and at the same time there should > be enough material to write decent literature review. > If you have recently successfully defended your proposal or just have > an interesting idea please write me. > Thanks for your help. > Anton > The idea of a qualifying exam is for you to prove that you can come up with an original proposal on your own and then successfully defend it. Anyone that gives you an idea is doing you, as well as the rest of the research community, a disfavor. A PhD is supposed to imply that you are capable of original thought. If I knew your proposal resulted from a request of this sort, no matter how good it was, I would fail you on the spot. ------------------------------------------------------------------------------- Siegfried Musser, Ph.D. Division of Biological Sciences, Section of Plant Biology University of California Davis, CA 95616 phone: (916) 752-8859 FAX: (916) 752-5410 smmusser@ucdavis.edu From owner-biophysics@net.bio.net Fri Jan 10 22:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!su-news-hub1.bbnplanet.com!newsxfer3.itd.umich.edu!howland.erols.net!math.ohio-state.edu!uwm.edu!newsspool.doit.wisc.edu!news.doit.wisc.edu!ebv.oncology.wisc.edu!aiyar From: aiyar@ebv.oncology.wisc.edu (Ashok Aiyar) Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Subject: Re: What is the SMALLEST DNA BINDING DOMAIN? Date: 11 Jan 1997 21:24:21 GMT Organization: Sugden Lab, McArdle Laboratory for Cancer Research, UW-Madison Lines: 25 Message-ID: References: Reply-To: aiyar@ebv.oncology.wisc.edu NNTP-Posting-Host: ebv.oncology.wisc.edu X-Newsreader: slrn (0.9.2.1 BETA UNIX) Xref: biosci bionet.biophysics:2536 bionet.molbio.proteins:9705 bionet.info-theory:4523 bionet.molbio.evolution:5508 bionet.molbio.methds-reagnts:53387 On Sat, 11 Jan 1997 11:52:26 EST, jstrassw@OPAL.TUFTS.EDU wrote: >Well, the Bovine Papillomavirus DNA binding domain (minimal) iosonlt 85 AA >although tit forms a dimer. It has the aadvantage of many characterized >mutations, and an excellent crystal structure (1.2A) and binds >sequence-specific to a palindropme at 10 -12 M. > >I also study it! The Kd value that you have listed for the minimal portion of "BPV-E2 DNA binding domain" binding it's cognate site is very different from that which has been published. For instance, from J. Virol. 71:828-831 (1997) (a publication from the Androphy lab), the Kd of the 87 a.a minimal DNA binding domain for it's cognate site is listed as approximately 7.0 nM (i.e. 7 x 10e-9 M), while a larger fragment, containing 127 a.a has a Kd of about 0.9 nM, and even this is quite far from the picomolar affinity you have listed .... Later, Ashok -- Ashok Aiyar, Ph.D. McArdle Laboratory for Cancer Research University of Wisconsin-Madison From owner-biophysics@net.bio.net Fri Jan 10 22:00:00 1997 Path: biosci!daresbury!nntp-trd.UNINETT.no!hermod.uio.no!nntp.uio.no!news.nacamar.de!news.icm.edu.pl!hammer.uoregon.edu!news-xfer.netaxs.com!feed1.news.erols.com!howland.erols.net!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.tufts.edu!opal.tufts.edu!jstrassw From: Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Subject: Re: What is the SMALLEST DNA BINDING DOMAIN? Date: Sat, 11 Jan 1997 11:52:26 EST Organization: Tufts University Lines: 31 Message-ID: References: NNTP-Posting-Host: opal.tufts.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII To: MThompson In-Reply-To: Xref: biosci bionet.biophysics:2535 bionet.molbio.proteins:9704 bionet.info-theory:4522 bionet.molbio.evolution:5507 bionet.molbio.methds-reagnts:53381 Hello! Well, the Bovine Papillomavirus DNA binding domain (minimal) iosonlt 85 AA although tit forms a dimer. It has the aadvantage of many characterized mutations, and an excellent crystal structure (1.2A) and binds sequence-specific to a palindropme at 10 -12 M. I also study it! John ------------------------------------------------------------------------ John Strasswimmer, MD,PhD Candidate | Phone (617) 636 8396 Tufts - New England Medical Center | Fax (617) 636 6190 Box 166 | Email: jstrassw@opal.tufts.edu Boston, MA 02111 USA | HTTP://WWW.healthsci.tufts.edu/microbiology/strass/JSpage.HTML *** Co-Author of "HyperBug" Microbiology Teaching Software *** | Remember, Your employer is eavesdropping... ------------------------------------------------------------------------ On Fri, 10 Jan 1997, MThompson wrote: > I am searching for a small independently folding (or in presence of a > metal ion), DNA binding domain. Would like it to be sequence specific, but > would greatly appreciate all suggestions. Be as detailed as possible or > please cite a reference, or name that I can locate it in the literature. > Thanks very much. > > From owner-biophysics@net.bio.net Fri Jan 10 22:00:00 1997 Path: biosci!agate!spool.mu.edu!newspump.sol.net!howland.erols.net!newsfeed.internetmci.com!news.infi.net!usenet From: cmarlowe@internic.net (Christopher Marlowe) Newsgroups: bionet.biophysics Subject: *****Wanted: Medical Physicist***** Date: 11 Jan 1997 16:19:17 GMT Organization: InfiNet Lines: 14 Message-ID: <5b8ei5$h9d@nw101.infi.net> NNTP-Posting-Host: pa20dsp11.orf.infi.net X-Newsreader: WinVN version 0.82 Junior Medical Physicist: Provide medical physics support for the Department of Radiation Oncology, Nuclear Medicine and Diagnostic Radiology. Duties include equipment calibration, quality control, radiation surveys, shielding designs, image analysis, clinical dosimetry of external beams, patient set-up, and computer treatment planning. Must have Ph.D. in Biomedical Engineering and 2 years experience with the above-described duties. US$ 57,500/yr; 40 hr/wk; 8:30 am - 4:30 pm; job in Chesapeake, Virginia USA. REPLY ONLY TO : AD NUMBER 707, THE VIRGINIAN-PILOT PO BOX 1319, NORFOLK, VIRGINIA 23501 From owner-biophysics@net.bio.net Sat Jan 11 22:00:00 1997 Path: biosci!fcs280s.ncifcrf.gov!not-for-mail From: toms@ncifcrf.gov (Tom Schneider) Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Subject: Re: What is the SMALLEST DNA BINDING DOMAIN? Followup-To: bionet.info-theory Date: 12 Jan 1997 00:25:49 GMT Organization: NCI-FCRDC Frederick Biomedical Supercomputing Center Lines: 17 Message-ID: <5b9b2d$2gg6@ncisun1-nf0.ncifcrf.gov> References: NNTP-Posting-Host: kaylor.ncifcrf.gov X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Xref: biosci bionet.biophysics:2537 bionet.molbio.proteins:9707 bionet.info-theory:4525 bionet.molbio.evolution:5509 bionet.molbio.methds-reagnts:53389 jstrassw@OPAL.TUFTS.EDU wrote: : Well, the Bovine Papillomavirus DNA binding domain (minimal) iosonlt 85 AA : although tit forms a dimer. It has the aadvantage of many characterized : mutations, and an excellent crystal structure (1.2A) and binds : sequence-specific to a palindropme at 10 -12 M. : : I also study it! Cool! What's the sequence logo look like? Tom Schneider National Cancer Institute Laboratory of Mathematical Biology Frederick, Maryland 21702-1201 toms@ncifcrf.gov http://www-lmmb.ncifcrf.gov/~toms/ From owner-biophysics@net.bio.net Sat Jan 11 22:00:00 1997 Path: biosci!rutgers!news.sgi.com!mr.net!newsfeeds.sol.net!hammer.uoregon.edu!news.icm.edu.pl!uw.edu.pl!news.nask.pl!01-newsfeed.univie.ac.at!swidir.switch.ch!news.grnet.gr!news.auth.gr!usenet From: "Stavros P. Zanos" Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.immunology,bionet.molbio.proteins,bionet.molecules.peptides,bionet.neuroscience,sci.med.pharmacy Subject: Phosphorylation of G-proteins Date: 12 Jan 1997 10:15:11 GMT Organization: Medical School, Thessaloniki Lines: 15 Message-ID: <01bc000c$e56f1080$0f02000a@slirp.hol.gr> NNTP-Posting-Host: antigoni.med.auth.gr X-Newsreader: Microsoft Internet News 4.70.1155 Xref: biosci bionet.biophysics:2540 bionet.cellbiol:6398 bionet.immunology:10642 bionet.molbio.proteins:9709 bionet.molecules.peptides:598 bionet.neuroscience:17653 sci.med.pharmacy:39351 It has come to my knowledge that G proteins undergo phosphorylation by cAMP- and calcium-dependent protein kinases and by tyrosine kinases. Having undertaken the writing of a part of a seminar on autonomic NS pharmacology, I was wandering if anyone out there knew something about the physiological role of G protein phosphorylation, especially in relation to neurotransmission and synaptic plasticity processes. Any details on the phosphorylation sites and regulation are also welcome. References to book chapters or journal articles are welcome. Thank you in advance. S.P. Zanos Aristotle U. Med. School Thessaloniki, Greece From owner-biophysics@net.bio.net Sat Jan 11 22:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!vixen.cso.uiuc.edu!uwm.edu!newsspool.doit.wisc.edu!news.doit.wisc.edu!daemon From: klenchin@facstaff.wisc.edu (Dima Klenchin) Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Subject: Re: What is the SMALLEST DNA BINDING DOMAIN? Date: Sun, 12 Jan 97 07:45:45 GMT Organization: UW-Madison Lines: 29 Message-ID: <5ba4tt$22b0@news.doit.wisc.edu> References: NNTP-Posting-Host: f181-185.net.wisc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=KOI8-R Content-Transfer-Encoding: 8bit X-No-Archive: Yes Xref: biosci bionet.biophysics:2539 bionet.molbio.proteins:9708 bionet.info-theory:4529 bionet.molbio.evolution:5511 bionet.molbio.methds-reagnts:53393 In article , aiyar@ebv.oncology.wisc.edu wrote: #On Sat, 11 Jan 1997 11:52:26 EST, jstrassw@OPAL.TUFTS.EDU # wrote: # #>Well, the Bovine Papillomavirus DNA binding domain (minimal) iosonlt 85 AA #>although tit forms a dimer. It has the aadvantage of many characterized #>mutations, and an excellent crystal structure (1.2A) and binds #>sequence-specific to a palindropme at 10 -12 M. #> #>I also study it! # #The Kd value that you have listed for the minimal portion of "BPV-E2 #DNA binding domain" binding it's cognate site is very different from #that which has been published. # #For instance, from J. Virol. 71:828-831 (1997) (a publication from #the Androphy lab), the Kd of the 87 a.a minimal DNA binding domain #for it's cognate site is listed as approximately 7.0 nM (i.e. 7 x 10e-9 M), #while a larger fragment, containing 127 a.a has a Kd of about 0.9 nM, #and even this is quite far from the picomolar affinity you have listed .... Umm, I know nothing about DNA binding, but biology is not a quantitative science, so 3 orders of magnitude makes no difference (since nobody knows how to apply those numbers anyway). Couldn't resist, - Dima From owner-biophysics@net.bio.net Sat Jan 11 22:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!news.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.mindspring.com!cssun.mathcs.emory.edu!news-feed-1.peachnet.edu!hobbes.cc.uga.edu!ice!jkenny From: jkenny@ice.bae.uga.edu (James Steven Kenny) Newsgroups: bionet.neuroscience,bionet.biophysics Subject: LaCl3 Date: 12 Jan 1997 02:08:23 GMT Organization: Biological & Agricultural Eng, UGA Lines: 39 Distribution: usa Message-ID: <5b9h2n$8gi@hobbes.cc.uga.edu> NNTP-Posting-Host: ice.bae.uga.edu Xref: biosci bionet.neuroscience:17649 bionet.biophysics:2538 I'm having problems with precipitation forming in a Tris Buffer with LaCl3. The precipitate forms slowly on the gas-liquid interface and is apparent after a period of 12-24 hours. The formation of the precipitate is pH dependent. Precipitation does not occur at pH below approximately 6.7. The amount of precipitation increases with increasing pH. Furthermore, the precipitation seems to be dependent upon the presence of atmospheric gasses. If argon is used to purge atmospheric gasses from the Tris Buffer and the solution is capped to prohibit gas transfer, precipitation does not occur. Lanthanum is a common tool to study the transport of Ca2+. I have read several references that use LaCl3 in Tris buffers at concentrations 1 to 2 orders of magnitude greater than the concentrations I am currently using with no mention of precipitation problems. Does anyone have experience using LaCl3 in a Tris buffer? Can anyone explain the precipitation and help me find a solution to this problem? Buffer Components: 10mM TrisHCl 0.8mM MgCl2 1.0mM CaCl2 5.3mM KCl 140mM NaCl 0.1mM LaCl3 pH adjusted with NaOH to a final pH of 7.4 Thank you for any help. Steve Kenny email: jkenny@bae.uga.edu -- ----------------------------------------------------------------------------- J. Steven Kenny E-mail: jkenny@ice.bae.uga.edu WWW: http://www.bae.uga.edu/dept/grads/jkenny From owner-biophysics@net.bio.net Sun Jan 12 22:00:00 1997 Path: biosci!daresbury!nntp-trd.UNINETT.no!online.no!sn.no!nntp.uio.no!news.apfel.de!news-fra1.dfn.de!news-koe1.dfn.de!main.Germany.EU.net!Dortmund.Germany.EU.net!Germany.EU.net!EU.net!news.bbnplanet.com!cpk-news-hub1.bbnplanet.com!feed1.news.erols.com!howland.erols.net!math.ohio-state.edu!uwm.edu!newsspool.doit.wisc.edu!news.doit.wisc.edu!daemon From: klenchin@facstaff.wisc.edu (Dima Klenchin) Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Subject: Re: What is the SMALLEST DNA BINDING DOMAIN? Date: Mon, 13 Jan 97 15:30:00 GMT Organization: UW-Madison Lines: 47 Message-ID: <5bdkgf$228m@news.doit.wisc.edu> References: <5ba4tt$22b0@news.doit.wisc.edu> <5bcd2r$2gg8@ncisun1-nf0.ncifcrf.gov> NNTP-Posting-Host: f182-050.net.wisc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=KOI8-R Content-Transfer-Encoding: 8bit X-No-Archive: Yes Xref: biosci bionet.biophysics:2547 bionet.molbio.proteins:9723 bionet.info-theory:4539 bionet.molbio.evolution:5525 bionet.molbio.methds-reagnts:53412 In article <5bcd2r$2gg8@ncisun1-nf0.ncifcrf.gov>, toms@ncifcrf.gov (Tom Schneider) wrote: #Dima Klenchin (klenchin@facstaff.wisc.edu) wrote: #: In article , # aiyar@ebv.oncology.wisc.edu wrote: #: #On Sat, 11 Jan 1997 11:52:26 EST, jstrassw@OPAL.TUFTS.EDU #: # wrote: #: # #: #>Well, the Bovine Papillomavirus DNA binding domain (minimal) iosonlt 85 AA #: #>although tit forms a dimer. It has the aadvantage of many characterized #: #>mutations, and an excellent crystal structure (1.2A) and binds #: #>sequence-specific to a palindropme at 10 -12 M. #: #the Kd of the 87 a.a minimal DNA binding domain #: #for it's cognate site is listed as approximately 7.0 nM (i.e. 7 x 10e-9 M), #: #while a larger fragment, containing 127 a.a has a Kd of about 0.9 nM, #: #and even this is quite far from the picomolar affinity you have listed .... #: #: Umm, I know nothing about DNA binding, but biology is not a quantitative #: science, so 3 orders of magnitude makes no difference (since nobody knows #: how to apply those numbers anyway). # #It makes a big difference, and those numbers can be applied, using advanced #molecular machine theory. I'll present the results later in several new #papers that are almost finished. If you would like to prepare yourself for #reading the new papers, read the ccmm, edmm and nano2 papers. If THOSE don't #make sense yet, read the earlier papers on binding site information theory #and sequence logos. All papers are available through my web site. Of course they do. There are all sorts of different things one can do comparing numbers. However, going back to the obscured to me Bovine Papillomavirus DNA binding domain, I'd like to ask one Q. Will our understanding of the biology of this virus change to _any_ extent should someone prove that affinity is 1 pM instead of 1 nM (or vice versa)? I bet - no. Sure, it'll make a lot of difference to people studying what combination of aa in what milieu works best when binding to a given structure, but for biology of the virus... Nowhere in the viral lifecycle we can apply those numbers to derive new and meaningful information about this organism. Thanks for reply, Tom. Intersting WEB site. I'll try to read the papers. I sure liked the idea of "No Consensus Sequences!" cause they make less and less sense as we go along the unknowns. . - Dima From owner-biophysics@net.bio.net Sun Jan 12 22:00:00 1997 Message-ID: <32DA75F8.1B31@nf.sympatico.ca> Date: Mon, 13 Jan 1997 09:50:48 -0800 From: Peter Beamish Organization: Ceta-Research X-Mailer: Mozilla 2.02E-SYMPA (Win95; I; 16bit) MIME-Version: 1.0 Newsgroups: bionet.biophysics Subject: TIME Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit NNTP-Posting-Host: 207.164.147.211 Lines: 66 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!newsfeed.internetmci.com!newsfeed.direct.ca!nntp.portal.ca!news.bc.net!torn!news1.bellglobal.com!endeavor.newcomm.net!207.164.147.211 Postscript # 64 to the "Lanzarote" paper (01/11/97) "TIME" By Peter Beamish Ph.D. Time has never been properly defined. It is the enigma of both modern physics and philosophy. This reality now appears to be partly because we need new definitions for "scalar and vector quantities" as well as the total number of "dimensions" permissible in the universe in which we live. The latter is THREE orthogonal (at right angle) dimensions. It is impossible for the human mind to comprehend a fourth dimension that is at right angles to the other three! We can imagine four variables, in fact superstring theory requires ten variables, BUT THERE ARE ONLY THREE DIMENSIONS. The mathematics of "n-variable" matrices is sound, useful and necessary for updating a theory combining general relativity and quantum mechanics, the so called quantum theory of gravity. But the n-variables must fit into three dimensions of space or, as I will soon suggest, into three dimensions of "space-time." A problem with defining time stems from an unclear definition of scalar and vector quantities. In classical physics texts a "scalar" has magnitude only, whereas a "vector" has both magnitude and direction. But direction is not the only property of space (as shown by Einstein). And, direction can be attained by simply adding a prerequisite to magnitude that it varies in the "direction" upward or say "forward" (1 to 10) or, downward or say "backward" (10 to 1 or -1 to -10). In fact it is the nature of time in its classical sense (time associated with the rotation of the Earth) that a measurement of its scalar property is always forward (with possibly very rare exceptions in studies of antimatter). This has been known as the "Arrow of Time," which becomes a vector if we add the new prerequisite above which thereby converts a scalar into a vector. But if the Arrow of Time is a newly defined vector then in what spacial direction or dimension does this arrow point? This is the clue that leads directly to "three dimensional time." Newtonian physics is missing two dimensions of time and these dimensions by analogy to the three dimensions of space must be orthogonal to the Arrow of Time. The western world mapped the rotation of the Earth onto the Arrow of Time by Euclidian geometry. But most of the remainder of the world, knowing little of this geometry more commonly used a different type of time, one based on cyclical, physiological properties (postscript # 30). Cycles are two dimensional, analogous to rotating wheels. And these cycles are often of a different period than that of the Earth. For example the timing of human breathing is obviously independent of the timing of the Earth's rotation. We have thus defined two types of time and both are vectors based on the above definition; they total to three dimensions which are necessarily at right angles. This is a coordinate system with a rotating wheel, always in the present, but moving "forward" along the axis of classical time. It can be superimposed onto the three space coordinates to form space-time in three dimensions. When this happens ten variables emerge to satisfy the superstring theories. There are three space variables or dimensions and seven time variables, three as dimensions and four as Rhythm Based Communication, "window" variables on the gauge symmetric wheel of the new "Rhythm Based Time" (diagram 2 in "Lanzarote" paper *). This circle can be of any diameter so that it will satisfy the very small microphysics of quantum theory as well as the very large macrophysics of relativity and cosmology. * The "Lanzarote" paper entitled "Rhythm Based Communication," published by the Secretariat of the World Conference on Sustainable Ecosystems, is available from the above author for a contribution to ongoing communications research, including cetaceans, terrestrial mammals, birds (and potentially kingdom Plantae) at: Ceta-Research Inc, Box 10, Trinity, NFLD.A0C 2S0 Canada. There are 64 postscript abstracts relating to General Interpretations, Applied Uses, Biophysics, Medicine, Mathematics, Music, Quantum Physics and Spirituality. From owner-biophysics@net.bio.net Sun Jan 12 22:00:00 1997 Path: biosci!UCONNVM.UCONN.EDU!RHODES From: RHODES@UCONNVM.UCONN.EDU Newsgroups: bionet.biophysics Subject: Re: Looking for ideas for qualification exam proposal Date: 13 Jan 1997 04:48:18 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <970113.074831.EST.RHODES@UConnVM.UConn.Edu> NNTP-Posting-Host: net.bio.net think about it - if you can't think of any interesting topics in your chosen field, why did you choose it? | O==O | | DAVID G. RHODES O==O PHONE 860-486-5413 | | SCHOOL OF PHARMACY; U-92 O==O FAX 860-486-4998 | | UNIVERSITY OF CONNECTICUT O==O | | STORRS, CT 06269-2092 O==O RHODES@UCONNVM.UCONN.EDU | | O==O | From owner-biophysics@net.bio.net Sun Jan 12 22:00:00 1997 Path: biosci!fcs280s.ncifcrf.gov!not-for-mail From: toms@ncifcrf.gov (Tom Schneider) Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Subject: Re: What is the SMALLEST DNA BINDING DOMAIN? Followup-To: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Date: 13 Jan 1997 04:32:07 GMT Organization: NCI-FCRDC Frederick Biomedical Supercomputing Center Lines: 17 Message-ID: <5bcds7$2gg10@ncisun1-nf0.ncifcrf.gov> References: <5bcck5$ho9@nntp3.u.washington.edu> NNTP-Posting-Host: kaylor.ncifcrf.gov X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Xref: biosci bionet.biophysics:2544 bionet.molbio.proteins:9716 bionet.info-theory:4537 bionet.molbio.evolution:5517 bionet.molbio.methds-reagnts:53403 Angeline Kantola (kantola@u.washington.edu) wrote: : C2H2 zinc fingers are everywhere (there are other kinds of zinc fingers as : well), so finding information about them should be easy. Mia Schmiedeskamp : and Rachel Klevit wrote a review of zinc fingers a few years back, which : is probably a good place to start. Thanks for the tip. I could not locate this in medline entrez. Schmiedeskamp does appear but is the wrong topic. R. Klevit wrote a number of papers on the subject. Tom Schneider National Cancer Institute Laboratory of Mathematical Biology Frederick, Maryland 21702-1201 toms@ncifcrf.gov http://www-lmmb.ncifcrf.gov/~toms/ From owner-biophysics@net.bio.net Sun Jan 12 22:00:00 1997 Path: biosci!fcs280s.ncifcrf.gov!not-for-mail From: toms@ncifcrf.gov (Tom Schneider) Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Subject: Re: What is the SMALLEST DNA BINDING DOMAIN? Followup-To: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Date: 13 Jan 1997 04:18:35 GMT Organization: NCI-FCRDC Frederick Biomedical Supercomputing Center Lines: 40 Message-ID: <5bcd2r$2gg8@ncisun1-nf0.ncifcrf.gov> References: <5ba4tt$22b0@news.doit.wisc.edu> NNTP-Posting-Host: kaylor.ncifcrf.gov X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Xref: biosci bionet.biophysics:2543 bionet.molbio.proteins:9715 bionet.info-theory:4535 bionet.molbio.evolution:5516 bionet.molbio.methds-reagnts:53402 Dima Klenchin (klenchin@facstaff.wisc.edu) wrote: : In article , aiyar@ebv.oncology.wisc.edu wrote: : #On Sat, 11 Jan 1997 11:52:26 EST, jstrassw@OPAL.TUFTS.EDU : # wrote: : # : #>Well, the Bovine Papillomavirus DNA binding domain (minimal) iosonlt 85 AA : #>although tit forms a dimer. It has the aadvantage of many characterized : #>mutations, and an excellent crystal structure (1.2A) and binds : #>sequence-specific to a palindropme at 10 -12 M. : #> : #>I also study it! : # : #The Kd value that you have listed for the minimal portion of "BPV-E2 : #DNA binding domain" binding it's cognate site is very different from : #that which has been published. : # : #For instance, from J. Virol. 71:828-831 (1997) (a publication from : #the Androphy lab), the Kd of the 87 a.a minimal DNA binding domain : #for it's cognate site is listed as approximately 7.0 nM (i.e. 7 x 10e-9 M), : #while a larger fragment, containing 127 a.a has a Kd of about 0.9 nM, : #and even this is quite far from the picomolar affinity you have listed .... : : Umm, I know nothing about DNA binding, but biology is not a quantitative : science, so 3 orders of magnitude makes no difference (since nobody knows : how to apply those numbers anyway). It makes a big difference, and those numbers can be applied, using advanced molecular machine theory. I'll present the results later in several new papers that are almost finished. If you would like to prepare yourself for reading the new papers, read the ccmm, edmm and nano2 papers. If THOSE don't make sense yet, read the earlier papers on binding site information theory and sequence logos. All papers are available through my web site. Tom Schneider National Cancer Institute Laboratory of Mathematical Biology Frederick, Maryland 21702-1201 toms@ncifcrf.gov http://www-lmmb.ncifcrf.gov/~toms/ From owner-biophysics@net.bio.net Sun Jan 12 22:00:00 1997 Path: biosci!news.artemis.com!uunet!in2.uu.net!140.142.64.3!news.u.washington.edu!kantola From: kantola@u.washington.edu (Angeline Kantola) Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Subject: Re: What is the SMALLEST DNA BINDING DOMAIN? Date: 13 Jan 1997 04:10:45 GMT Organization: University of Washington, Seattle Lines: 24 Message-ID: <5bcck5$ho9@nntp3.u.washington.edu> References: NNTP-Posting-Host: carson.u.washington.edu Xref: biosci bionet.biophysics:2542 bionet.molbio.proteins:9714 bionet.info-theory:4534 bionet.molbio.evolution:5515 bionet.molbio.methds-reagnts:53401 In article , MThompson wrote: >I am searching for a small independently folding (or in presence of a >metal ion), DNA binding domain. Would like it to be sequence specific, but >would greatly appreciate all suggestions. Be as detailed as possible or >please cite a reference, or name that I can locate it in the literature. >Thanks very much. Cys2-His2 zinc fingers are on the order of 30 amino acids long. As the name suggests, a zinc atom is critical for structural integrity of the functional domain. Proteins which bind DNA via C2H2 zinc fingers do so sequence specifically. There's no evidence that single zinc fingers bind nucleic acids, though. A minimum of three of these fingers are required to bind or, as in the case of yeast ADR1, two fingers and a non-finger N termial region. C2H2 zinc fingers are everywhere (there are other kinds of zinc fingers as well), so finding information about them should be easy. Mia Schmiedeskamp and Rachel Klevit wrote a review of zinc fingers a few years back, which is probably a good place to start. Best of luck, Angie Kantola From owner-biophysics@net.bio.net Sun Jan 12 22:00:00 1997 Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.molbio.evolution Path: biosci!daresbury!nntp-trd.UNINETT.no!sn.no!hermod.uio.no!nntp.uio.no!news.maxwell.syr.edu!arclight.uoregon.edu!news.bc.net!nntp.mbnet.mb.ca!utcsri!utgpu!utinfo!bloch!gardner From: gardner@bloch (Kevin Gardner) Subject: Re: What is the SMALLEST DNA BINDING DOMAIN? X-Nntp-Posting-Host: bloch.med.utoronto.ca Message-ID: Followup-To: bionet.biophysics,bionet.molbio.proteins,bionet.molbio.evolution Sender: nntp@utcc.utoronto.ca (News) Organization: UTCC Campus Access X-Newsreader: TIN [version 1.2 PL2] References: <5bcck5$ho9@nntp3.u.washington.edu> Date: Mon, 13 Jan 1997 16:59:38 GMT Lines: 53 Xref: biosci bionet.biophysics:2548 bionet.molbio.proteins:9725 bionet.molbio.evolution:5528 (Followups trimmed) Angeline Kantola (kantola@u.washington.edu) wrote: : Cys2-His2 zinc fingers are on the order of 30 amino acids long. As the : name suggests, a zinc atom is critical for structural integrity of the : functional domain. Proteins which bind DNA via C2H2 zinc fingers do so : sequence specifically. There's no evidence that single zinc fingers bind : nucleic acids, though. A minimum of three of these fingers are required to : bind or, as in the case of yeast ADR1, two fingers and a non-finger N : termial region. : C2H2 zinc fingers are everywhere (there are other kinds of zinc fingers as : well), And I'd like to bring up one of those other kinds of zinc fingers: the Zn(2)Cys(6) binuclear cluster class, whose best known member is the S. cerevisiae GAL4 transcriptional activator. Somewhat similarly to the Cys2-His2 group above, the part of the domain that binds DNA and 2*Zn(II) is about 30aa in length and recognizes a 5'-CGG-3' triplet. This is somewhat of an oversimplification, though. Similarly to the Cys2-His2 case, specificity is derived from using multiple domains; in contrast, though, these domains are non-covalently dimerized, making the spacing between two inverted CGG elements as the prime source of specificity. When you add the additional linker & dimerization helix onto the DNA-binding domain, the total length kicks up to ca. 50-60aa. References: aside from my own work (NMR studies of GAL4 and LAC9, a K. lactis GAL4 homologue :), I'd check into: *specificity: several recent papers by Aseem Ansari in Mark Ptashne's group and references therein. *sequence homology in group: alignment paper in NAR last month (NAR 24(1996): 4599-4607) *structural biology: two crystal structures of protein DNA/complexes by Ronan Marmorstein when he was in Steve Harrison's group (GAL4 and PPR1); additional NMR studies by several groups including Gerhard Wagner, Ernest Laue, Joe Coleman and others. Cheers, Kevin -- ************************************************************************* Kevin Gardner gardner@bloch.med.utoronto.ca University of Toronto http://abragam.med.utoronto.ca/~gardner Dept. of Medical Genetics & Microbiology phone: 416-978-0642/FAX: -6885 From owner-biophysics@net.bio.net Sun Jan 12 22:00:00 1997 Path: biosci!daresbury!lyra.csx.cam.ac.uk!uknet!usenet1.news.uk.psi.net!uknet!www.nntp.primenet.com!nntp.primenet.com!dciteleport.com!feed1.news.erols.com!news.idt.net!nntp.farm.idt.net!news From: cybek@mail.idt.net (William Theaux) Newsgroups: bionet.biophysics Subject: Laymen’s association search for DNA information Date: Mon, 13 Jan 1997 22:06:45 GMT Organization: IDT Lines: 9 Message-ID: <5bebhm$7ql@nnrp1.farm.idt.net> Reply-To: cybek@mail.idt.net NNTP-Posting-Host: ppp-31.ts-15.nyc.idt.net X-Newsreader: Forte Free Agent 1.0.82 Independant association of people who want to preserve their DNA by themselves are searching for conservation technic. We have heard about some very simple and safe non-cryogenic proceduresf - anyone with information, please contact us. Thanks. CYBEK\William Theaux From owner-biophysics@net.bio.net Mon Jan 13 22:00:00 1997 Path: biosci!TTUHSC.EDU!phyan From: phyan@TTUHSC.EDU (Alan Neely) Newsgroups: bionet.biophysics Subject: Re: =?iso-8859-1?Q?Laymen=92s?= association search for DNA information Date: 13 Jan 1997 16:43:38 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 32 Sender: daemon@net.bio.net Distribution: world Message-ID: <199701140043.QAA17024@net.bio.net> NNTP-Posting-Host: net.bio.net At 10:06 PM 1/13/97 GMT, you wrote: > > >Independant association of people who want to preserve their DNA by >themselves are searching for conservation technic. We have heard about >some very simple and safe non-cryogenic proceduresf - anyone with >information, please contact us. Thanks. > >CYBEK\William Theaux > > If you are so worried about immortality you better go to church. My first inclination will be to strongly discourage people from wasting time and resource in this endeavour. I do not see the use for it. Your body is a pretty safe place to conserve DNA it would last long after you are burried and your offspring carried a large portion of it. On the other hand your DNA may contain incriminating information like oncogene, the gene for alcohol addiction, homosexuality, race, stupidity etc... While in your body your DNA is protected by the constitution. In a test tube the DNA is up for grab. Alan Neely, Ph.D. Assistant Professor Dep. of Physiology TTUHSC,Lubbock, Texas From owner-biophysics@net.bio.net Mon Jan 13 22:00:00 1997 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!math.ohio-state.edu!uwm.edu!newsfeeds.sol.net!news-xfer.netaxs.com!cs.utexas.edu!atlantis.utmb.edu!news From: bdodson@beowulf.utmb.edu (M. L. Dodson) Newsgroups: bionet.biophysics,bionet.jobs,bionet.molbio.gdb,bionet.molbio.genbank,bionet.molbio.methds-reagents,bionet.molbio.proteins,bionet.molec-model Subject: Postdoctoral positions in rapid kinetics Date: 14 Jan 1997 20:46:16 GMT Organization: The Sealy Center for Molecular Science Lines: 19 Distribution: world Message-ID: <5bgrap$cn4@atlantis.utmb.edu> Reply-To: bdodson@beowulf.utmb.edu NNTP-Posting-Host: beowulf.utmb.edu Keywords: kinetics,enzymology,DNA,repair,polymerase Xref: biosci bionet.biophysics:2555 bionet.molbio.gdb:563 bionet.molbio.genbank:2481 bionet.molbio.proteins:9728 bionet.molec-model:1321 Two postdoctoral positions are currently available to carry out rapid kinetic analyses of both DNA repair enzymes and DNA polymerases on defined substrates. Experience with fast kinetics is essential. Send CV to: R. Stephen Lloyd Center for Molecular Science Rt. 1071 University of Texas Medical Branch Galveston, TX 77555-1071 or reply by email: kinetics@scms.utmb.edu UTMB is an affirmative action employer M/F/D/V. From owner-biophysics@net.bio.net Mon Jan 13 22:00:00 1997 Path: biosci!citi2.fr!bali From: bali@citi2.fr (Moez BALI) Newsgroups: bionet.biophysics Subject: Ancien de Biophysique moleculaire Date: 14 Jan 1997 11:03:12 -0800 Organization: INSERM U.426 Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: <32DBD8F9.2D9E@bisance.citi2.fr> Reply-To: bali@citi2.fr NNTP-Posting-Host: net.bio.net salut, Nous sommes actuellement en train de realiser un annuaire des anciens du DEA de biophysique moleculaire des universite Paris 6, 7, 13, Orleans et Ecole Centrale. -> Si vous etes un ancien : contacter moi, mon adresse est en dessous. Si vous connaissez un ancien de ce DEA, soyez assez aimable pour lui transmettre le message. Voila, j'espere ne pas avoir outrepasse les regles de net-etiquette en utilisant ce forum pour un message de ce type. -- ________________________________________________ Moez BALI Unite INSERM U.467 Biophysique des transports epitheliaux Faculte de Medecine Necker-Enfants Malades Tel. +33 01.43.06.15.25 ; 01.40.61.56.26 Fax +33 01.40.61.55.91 From owner-biophysics@net.bio.net Mon Jan 13 22:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!newsxfer3.itd.umich.edu!portc01.blue.aol.com!spamz.news.aol.com!audrey01.news.aol.com!not-for-mail From: connexin43@aol.com (Connexin43) Newsgroups: bionet.biophysics Subject: POSTDOCTORAL POSITION at Indiana-Purdue Medical Center Date: 14 Jan 1997 17:21:04 GMT Organization: AOL http://www.aol.com Lines: 12 Message-ID: <19970114171800.MAA25508@ladder01.news.aol.com> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com A position is open for graduated students interested in the study of the physiological regulation of gap junctions, in particular, those present in between heart cells. The candidate will work in the laboratory of Dr. Alonso P Moreno PhD. and is expected to be enthusiastic and committed to a productive carreer in Electrophysiology and some Molecular biology.Some projects are already funded and salary is negotiable. Interested contact Dr. Moreno at Krannert Institute of Cardiology 1111 West 10th Street Indianapolis IN. 46202 moreno@kimail.dmed.iupui.edu From owner-biophysics@net.bio.net Mon Jan 13 22:00:00 1997 Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Path: biosci!bcm.tmc.edu!cs.utexas.edu!news-xfer.netaxs.com!hammer.uoregon.edu!arclight.uoregon.edu!news.bc.net!nntp.mbnet.mb.ca!utcsri!utgpu!lamoran From: lamoran@gpu.utcc.utoronto.ca (L.A. Moran) Subject: Re: What is the SMALLEST DNA BINDING DOMAIN? Message-ID: Organization: UTCNS Public Access References: <5bcck5$ho9@nntp3.u.washington.edu> Date: Tue, 14 Jan 1997 14:49:42 GMT Lines: 64 Xref: biosci bionet.biophysics:2552 bionet.molbio.proteins:9726 bionet.info-theory:4547 bionet.molbio.evolution:5532 bionet.molbio.methds-reagnts:53450 In article <5bcck5$ho9@nntp3.u.washington.edu>, Angeline Kantola wrote: >In article , >MThompson wrote: >>I am searching for a small independently folding (or in presence of a >>metal ion), DNA binding domain. Would like it to be sequence specific, but >>would greatly appreciate all suggestions. Be as detailed as possible or >>please cite a reference, or name that I can locate it in the literature. This request brings up another issue that might be worth discussing. Should these DNA binding regions be referred to as "domains" or "motifs"? In the literature on protein structure the word "domain" usually refers to a discrete, independently folding region with a globular structure. Such domains consist of large stretches of primary sequence and they can be recognized as distinct units in the overall structure of a protein. Domains are usually connected by short linker sequences. Domains, as defined by structural biologists, consist of multiple regions of secondary or supersecondary structures. The supersecondary structures are often referred to as "motifs". Examples of motifs include helix-loop- helix regions, Greek keys, and beta-alpha-beta units. Some of these supersecondary structures form the core of a DNA binding region and that's why most textbooks refer to them as DNA binding "motifs". Examples are the helix-turn-helix motif and the zinc finger motif. A counter-example is the homeobox "domain"; a definition that is not consistent with the terminology used by those who study protein structure. It's not clear to me what MThompson is looking for. If it's really a distinct "domain" then most of those who responded have not been helpful since they have concentrated on "motifs". However, perhaps MThompson really wanted an example of such a motif? I realize that this might seem a bit picky but I think that it is important to be consistent with terminology and I'm pleased to see that even leading molecular biology textbooks have adopted the word "motif" to describe these DNA binding regions of protein. This makes the biochemists happy. (-: >Cys2-His2 zinc fingers are on the order of 30 amino acids long. As the >name suggests, a zinc atom is critical for structural integrity of the >functional domain. Proteins which bind DNA via C2H2 zinc fingers do so >sequence specifically. There's no evidence that single zinc fingers bind >nucleic acids, though. A minimum of three of these fingers are required to >bind or, as in the case of yeast ADR1, two fingers and a non-finger N >termial region. Zinc fingers and other zinc containing structures are examples of motifs and not, strictly speaking, domains. They are often part of a large domain within a protein. Motifs are usually short stretches of primary sequence on the order of 20-100 amino acid residues. Domains are larger. >C2H2 zinc fingers are everywhere (there are other kinds of zinc fingers as >well), so finding information about them should be easy. Mia Schmiedeskamp >and Rachel Klevit wrote a review of zinc fingers a few years back, which >is probably a good place to start. Some people are very sensitive about the naming of zinc containing structures. The C2H2 structure is called a zinc finger but the others are not referred to as "fingers". It is probably useful to maintain this distinction. Larry Moran From owner-biophysics@net.bio.net Mon Jan 13 22:00:00 1997 Path: biosci!rutgers!news.sgi.com!howland.erols.net!EU.net!uknet!usenet1.news.uk.psi.net!uknet!uknet!lyra.csx.cam.ac.uk!warwick!leicester!usenet From: "Dr E. Buxbaum" Newsgroups: bionet.biophysics Subject: Re: TIME Date: 14 Jan 1997 14:00:02 GMT Organization: University of Leicester, UK (PCFS User) Lines: 18 Message-ID: <5bg3h2$3ib@falcon.le.ac.uk> References: <32DA75F8.1B31@nf.sympatico.ca> NNTP-Posting-Host: pc5.msb.le.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 16bit) Peter Beamish wrote: >Postscript # 64 to the "Lanzarote" paper (01/11/97) > > "TIME" > By Peter Beamish Ph.D. > > Time has never been properly defined. It is the enigma of both >modern physics and philosophy. > This reality now appears to be partly because we need new >definitions for "scalar and vector quantities" as well as the total >number of "dimensions" permissible in the universe in which we live. >The latter is THREE orthogonal (at right angle) dimensions. It is >impossible for the human mind to comprehend a fourth dimension that is at >right angles to the other three! And just because we can not imagine more than 3 dimensions means that there can be no more? Humans as the measure of all things? From owner-biophysics@net.bio.net Mon Jan 13 22:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!howland.erols.net!vixen.cso.uiuc.edu!uwm.edu!msunews!gvsu!river.it.gvsu.edu!friedj From: "Jessica T. Fried" Newsgroups: bionet.biophysics Subject: a question about differential equations Date: Tue, 14 Jan 1997 18:43:03 -0500 Organization: Grand Valley State University Lines: 10 Message-ID: NNTP-Posting-Host: river.it.gvsu.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Thankyou for picking up this message! I'm looking for a topic to write a paper on in my 300 level Differential Equations class. I'm very interested in plants and would love to know if there are any SIMPLE applications of diff.eq. in botany. If anyone out there has any ideas, please let me know! -Jessica From owner-biophysics@net.bio.net Tue Jan 14 22:00:00 1997 Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!feed1.news.erols.com!arclight.uoregon.edu!news.bc.net!nntp.mbnet.mb.ca!utcsri!utgpu!utinfo!bloch!gardner From: gardner@bloch (Kevin Gardner) Subject: Domains and motifs X-Nntp-Posting-Host: bloch.med.utoronto.ca Message-ID: Followup-To: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Sender: nntp@utcc.utoronto.ca (News) Organization: UTCC Campus Access X-Newsreader: TIN [version 1.2 PL2] References: <5bcck5$ho9@nntp3.u.washington.edu> Date: Tue, 14 Jan 1997 20:26:28 GMT Lines: 60 Xref: biosci bionet.biophysics:2557 bionet.molbio.proteins:9729 bionet.info-theory:4550 bionet.molbio.evolution:5534 bionet.molbio.methds-reagnts:53465 Hi all: While my upstairs neightbor Larry (lamoran@gpu.utcc.utoronto.ca) brings up an interesting point in this discussion, I think we're on two different wavelengths on the definition of domains and motifs. Anyone else care to comment? L.A. Moran (lamoran@gpu.utcc.utoronto.ca) wrote: : This request brings up another issue that might be worth discussing. Should : these DNA binding regions be referred to as "domains" or "motifs"? In the : literature on protein structure the word "domain" usually refers to a : discrete, independently folding region with a globular structure. Such : domains consist of large stretches of primary sequence and they can be : recognized as distinct units in the overall structure of a protein. Domains : are usually connected by short linker sequences. OK --- looks good so far, although I don't agreed with the term "large stretches" of primary sequence. Some domains that don't bind ligands are as small as ~50-60 aa (SH3 domain, for example); if metals and/or disulfide bonds are present, the size can be smaller. : Domains, as defined by structural biologists, consist of multiple regions : of secondary or supersecondary structures. Fine. : Zinc fingers and other zinc containing structures are examples of motifs : and not, strictly speaking, domains. They are often part of a large domain : within a protein. Motifs are usually short stretches of primary sequence : on the order of 20-100 amino acid residues. Domains are larger. Whoa --- speaking as a structural biologist who has worked on DNA-binding domains such as these, I don't agree with this assessment. The Cys(2)His(2) canonical zinc finger and the GAL4/Cys(6) both fall into a domain by your criteria that you initially listed: discrete, independently-folding regions with globular structures. They're connected to other domains by linker regions, and both have multiple regions of secondary structure (2 strands of beta sheet and a helix for the Cys(2)His(2), 2 helices for GAL4). Yes, these domains require metal ion(s) in order to assume their folded, active state. Given this above information, I'd be interested in hearing how these would fail to be considered domains. Yes, they are small domains (as asked for), but I believe they meet the appropriate criteria. : Some people are very sensitive about the naming of zinc containing structures. : The C2H2 structure is called a zinc finger but the others are not referred : to as "fingers". It is probably useful to maintain this distinction. Being one of those people who's worked on the naming of zinc-containing structures that isn't a Cys(2)His(2) domain, I'd like to see this distinction maintained. But I also realized that the chances of this happening are essentially nil.... Kevin -- ************************************************************************* Kevin Gardner gardner@bloch.med.utoronto.ca University of Toronto http://abragam.med.utoronto.ca/~gardner Dept. of Medical Genetics & Microbiology phone: 416-978-0642/FAX: -6885 From owner-biophysics@net.bio.net Tue Jan 14 22:00:00 1997 Path: biosci!NOTES.compuserve.com!INTERNET#c#dozer.netwizards..net.CISHUB From: INTERNET#c#dozer.netwizards..net.CISHUB@NOTES.compuserve.com Newsgroups: bionet.biophysics Subject: A Special Offer! Date: 15 Jan 1997 07:14:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: <199701151014_MC1-E24-7A12@compuserve.com> NNTP-Posting-Host: net.bio.net Sender: BIOSCI-REQUEST@net.bio.net Received: from net.bio.net (net.bio.net [204.31.212.2]) by hil-img-6.compuserve.c om (8.6.10/5.950515) id EAA17828; Wed, 15 Jan 1997 04:50:16 -0500 Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id BAA17836; Wed, 15 Jan 1997 01:36:12 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id BAA17831; Wed, 1 5 Jan 1997 01:36:12 -0800 To: biophys@net.bio.net From: dozer@netwizards.net Subject: A Special Offer! Date: Tue, 14 Jan 1997 19:01:17 Message-ID: <5bhh9e$j6e@news1-alterdial.uu.net> NNTP-Posting-Host: cust68.max22.san-francisco.ca.ms.uu.net Are you interested in making Millions in your spare time? Any housewife could do it and so can you! Just email me at dozer@netwizards.net to receive your FREE information packet. Thanks for your attention, and I'll be talking with you soon. Bill dozer@netwizards.net From owner-biophysics@net.bio.net Tue Jan 14 22:00:00 1997 Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Path: biosci!daresbury!nntp-trd.UNINETT.no!sn.no!hermod.uio.no!nntp.uio.no!news-feed.inet.tele.dk!arclight.uoregon.edu!news.bc.net!nntp.mbnet.mb.ca!utcsri!utgpu!lamoran From: lamoran@gpu.utcc.utoronto.ca (L.A. Moran) Subject: Re: Domains and motifs Message-ID: Organization: UTCNS Public Access References: <5bcck5$ho9@nntp3.u.washington.edu> Date: Wed, 15 Jan 1997 16:48:04 GMT Lines: 92 Xref: biosci bionet.biophysics:2561 bionet.molbio.proteins:9737 bionet.info-theory:4556 bionet.molbio.evolution:5542 bionet.molbio.methds-reagnts:53493 In article , Kevin Gardner wrote: >Whoa --- speaking as a structural biologist who has worked on DNA-binding >domains such as these, I don't agree with this assessment. The >Cys(2)His(2) canonical zinc finger and the GAL4/Cys(6) both fall into >a domain by your criteria that you initially listed: discrete, >independently-folding regions with globular structures. They're connected >to other domains by linker regions, and both have multiple regions of >secondary structure (2 strands of beta sheet and a helix for the >Cys(2)His(2), 2 helices for GAL4). Yes, these domains require metal >ion(s) in order to assume their folded, active state. > >Given this above information, I'd be interested in hearing how these >would fail to be considered domains. Yes, they are small domains >(as asked for), but I believe they meet the appropriate criteria. I readily admit that the literature is confusing on this issue and many workers refer to helix-turn-helix "domains" and zinc finger "domains". The purpose of my posting was to point out that the protein structure experts often refer to these as "motifs" because the word "domain" has a different meaning. You will notice in the more recent literature and in textbooks that the word "motif" is gaining in popularity - rightly so in my opinion. (see the titles of Lee et al. (1989), Berg (1990), and Chan et al. (1993)). In Coleman's review of zinc proteins from 1992 he refers to zinc finger motifs in some cases but to domains in other cases so even five years ago there was some confusion in the literature. Let's see what Creighton has to say in his textbook. In chapter 8 he talks about DNA binding proteins and the various binding motifs including the zinc finger motif (p.358). He doesn't refer to these regions as domains because he defines domains quite differently, "The folded structures of most small proteins are roughly spherical and remarkably compact, with very irregular surfaces. The structures of most proteins that have more than about 200 residues appear to consist of two, three, or more structural units, usually referred to as domains. The domains of a protein molecule interact to varying degrees, but less extensively than do structural elements within domains. Often a single segment of a polypeptide chain links the domains, and each domain consists of a single stretch of polypeptide chain. ... The definition of a domain is not rigorous, and the division of a structure into domains is a subjective process that is done in different ways by different people. Other terms and subdivisions, such as subdomain and folding unit, are also encountered in the literature. Nevertheless, the presence of domains in many protein molecules is clear to all observers (Figures). Domains are most evident by their compactness, which can be expressed quantitatively as the ratio of the surface area of a domain to the surface area of a sphere with the same volume ..." Creighton (1993) p.219 As a general rule of thumb you should be able to recognize domains in a space-filling model of a protein. They form separate "blobs" that are quite obvious. If you can't pick out a specific region easily by looking at the gross morphology of the protein then it isn't a domain by Creighton's definition. Zinc fingers are not domains by this definition. A typical zinc finger contains three regions of secondary structure (two beta regions and an alpha helix). This is typical of many other supersecondary structures or motifs that have been recognized in proteins (ie: beta-alpha-beta units). There doesn't seem to be any convincing reason to call a zinc finger a domain but not other types of supersecondary structure. And there doesn't seem to be any logical reason to degrade the term "domain" when we have a perfectly good alternative in "motif". Larry Moran Berg, J.M. (1990) Zinc Fingers and Other Metal-Binding Domains. J. Biol. Chem. 265,6513-6516. Chan, Y-L., Suzuki, K., Olvera. J. and Wool, G. (1993) Zinc finger-like motifs in rat ribosomal proteins S27 and S29. Nucl. Acid. Res. 21,649. Creighton, T.E. (1993) Proteins: Structures and Molecular Properties. W. H. Freeeman, New York Coleman, J.E. (1992) Zinc Proteins: Enzymes, Storage Proteins, Transcription Factors, and Replication Proteins. Ann. Rev. Biochem. 61,897-946. Lee, M.S., Gippert, G.P., Soman, K.V., Case, D.A. and Wright, P.E. (1989) Three-Dimensional Solution Structure of a Single Zinc Finger DNA-Binding Domain. Sci. 245,635-637. From owner-biophysics@net.bio.net Tue Jan 14 22:00:00 1997 Path: biosci!agate!howland.erols.net!newsxfer3.itd.umich.edu!news.bbnplanet.com!su-news-hub1.bbnplanet.com!arclight.uoregon.edu!newsfeed.direct.ca!nntp.portal.ca!van-bc!n1van.istar!van.istar!west.istar!ott.istar!istar.net!tor.istar!east.istar!news.inforamp.net!news.nstn.ca!newsflash.concordia.ca!sunqbc.risq.net!athena.ulaval.ca!not-for-mail From: "Rydberg" Newsgroups: bionet.biophysics Subject: THz region experiments in biophysics Date: 15 Jan 1997 15:46:15 GMT Organization: Departement de Physique Lines: 11 Message-ID: <01bc02fc$0b9ec560$534ccb84@einstein.ulaval.ca> NNTP-Posting-Host: rydberg.phy.ulaval.ca X-Newsreader: Microsoft Internet News 4.70.1155 I would like to know if somebody has ever heard about experiments done in the far infrared region (THz) in biophysics? -- Christophe RIVIERE Centre d'Optique Photonique et Laser Universite Laval, Quebec, CANADA G1K 7P4 Tel.: + (418) 656-2131-3007 Fax:. + (418) 656-2623 Email: criviere@phy.ulaval.ca From owner-biophysics@net.bio.net Tue Jan 14 22:00:00 1997 Path: biosci!daresbury!lyra.csx.cam.ac.uk!news.ox.ac.uk!news From: Simon B Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Subject: Re: Domains and motifs Date: Wed, 15 Jan 1997 19:02:48 +0000 Organization: Psynix New Media Lines: 60 Message-ID: <32DD29D8.190D@bioch.ox.ac.uk> References: <5bcck5$ho9@nntp3.u.washington.edu> Reply-To: smb@psynix.co.uk NNTP-Posting-Host: max31.public.ox.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01Gold (Win95; I) Xref: biosci bionet.biophysics:2563 bionet.molbio.proteins:9738 bionet.info-theory:4559 bionet.molbio.evolution:5543 bionet.molbio.methds-reagnts:53497 Kevin Gardner wrote: > > Hi all: > While my upstairs neightbor Larry (lamoran@gpu.utcc.utoronto.ca) > brings up an interesting point in this discussion, I think we're on two > different wavelengths on the definition of domains and motifs. Anyone > else care to comment? > > L.A. Moran (lamoran@gpu.utcc.utoronto.ca) wrote: > : This request brings up another issue that might be worth discussing. Should > : these DNA binding regions be referred to as "domains" or "motifs"? In the > : literature on protein structure the word "domain" usually refers to a > : discrete, independently folding region with a globular structure. Such > : domains consist of large stretches of primary sequence and they can be > : recognized as distinct units in the overall structure of a protein. Domains > : are usually connected by short linker sequences. > > OK --- looks good so far, although I don't agreed with the term "large > stretches" of primary sequence. Some domains that don't bind ligands > are as small as ~50-60 aa (SH3 domain, for example); if metals and/or > disulfide bonds are present, the size can be smaller. > > : Domains, as defined by structural biologists, consist of multiple regions > : of secondary or supersecondary structures. > > Fine. > > : Zinc fingers and other zinc containing structures are examples of motifs > : and not, strictly speaking, domains. They are often part of a large domain > : within a protein. Motifs are usually short stretches of primary sequence > : on the order of 20-100 amino acid residues. Domains are larger. > Haven't read the whole discussion - sorry if this point has been made. The distinction between domains and motifs is not related to size, or number of residues etc. There is no physical size limit on a motif, it can be as small or as large as you want. There are, however, upper and lower physical size limits on domains (would be quite interesting to discuss the possible reasons for this, but it's a bit off-topic, so I won't) A motif is a feature that is commonly recurring. So a Zinc finger could be called a motif. But I don't see why it couldn't also be called a domain. A good definition of a protein domain is tricky - but something in terms of a strong (maybe or maybe not autonomous?) intrinsic propensity to fold to it's native form is a good way to think about it. Gotta go, Simon Simon M. Brocklehurst smb@bioch.ox.ac.uk OCMS, Dept. Biochemistry University of Oxford, UK From owner-biophysics@net.bio.net Tue Jan 14 22:00:00 1997 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!u-psud.fr!usenet From: Mark Blight Newsgroups: bionet.biophysics Subject: Do I need a Flame Spec? Date: Wed, 15 Jan 1997 19:40:42 +0100 Organization: Universite Paris-Sud, France. Lines: 54 Message-ID: <32DD24AA.284B@igmors.u-psud.fr> NNTP-Posting-Host: bmec2.igmors.u-psud.fr Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: quoted-printable X-Mailer: Mozilla 2.0 (Macintosh; I; 68K) Hello, I am a molecular biologist by training and so am a bit rusty on some of the= apparatus and = techniques that can be used for more chemical type studies. However, I am interested in determining the "purity" of a complex mixture (= a plant extract) = with respect to other contaminating mixtures from, for example, other plant= species. I also = need to process many samples with great rapidity and so organic extraction = and HPLC etc = are probably too slow. I was wondering if the absorption spectra (like for = stellar objects) of = a reference sample could be compared to an unknown and additional absorptio= n bands = subtracted to help identify the possible contaminant by comparison to a dat= abank of = potential contaminating substances (or mixtures). Like I said, I'm not very= familiar with = the techniques that might be employed or the equipment. So, would I need a = flame = spectrometer (or is that a photometer?) to generate the absorption spectra?= And can anyone = with more experience give me an idea if this might work? Thanks for any suggestions, Please reply by e-mail. Mark _______________________________________ Dr. Mark A. Blight, Institut de G=E9n=E9tique et Microbiologie, CNRS URA 1354, B=E2timent 409, Universit=E9 de Paris XI, 91405 Orsay cedex, France. Tel: +33 1 69 15 66 99 Fax: + 33 1 69 15 78 08 e-mail: BLIGHT@IGMORS.U-PSUD.FR _______________________________________ From owner-biophysics@net.bio.net Wed Jan 15 22:00:00 1997 Path: biosci!agate!howland.erols.net!ais.net!noc.van.hookup.net!nic.mtl.hookup.net!rcogate.rco.qc.ca!altitude!usenet From: "Achim Recktenwald, PhD" Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Subject: Re: What is the SMALLEST DNA BINDING DOMAIN? Date: Thu, 16 Jan 1997 07:48:34 -0500 Organization: IBEX Technologies, Inc., Biochemistry, 5485 Pare, Montreal, PQ, H4P 1P7, Canada Lines: 31 Message-ID: <32DE23A0.5840@ibex.ca> References: NNTP-Posting-Host: ibex.hip.cam.org Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Macintosh; I; PPC) Xref: biosci bionet.biophysics:2565 bionet.molbio.proteins:9747 bionet.info-theory:4564 bionet.molbio.evolution:5550 bionet.molbio.methds-reagnts:53528 MThompson wrote: > > I am searching for a small independently folding (or in presence of a > metal ion), DNA binding domain. Would like it to be sequence specific, but > would greatly appreciate all suggestions. Be as detailed as possible or > please cite a reference, or name that I can locate it in the literature. > Thanks very much. Check out the T4 endonuclease V; it's a DNA-repair enzyme, preparing thymine-dimers for the complete excision by the DNA polymerase. I worked on this enzyme about 7 - 8 years ago, when I was a post-doc in Japan. Unfortunately, it is nothing really known about the binding site, but assuming the longest axis of this small protein (~16kDa) is equivalent to the maximal number of basepairs that can bind, the number should be <= 8 pairs. We were never sure about the binding-site, because several of the really important site-directed mutants were not expressed by E. coli. However, there is a very good crystal structure available. The institute's name was 'Protein Engineering Research Institute (PERI)', but has now be changed to BERI. They have a web-page: http://www.beri.co.jp/ Look for papers with the names of Morikawa, Tomoko Doi and myself. There was also a competitors group in the US, but I forgot the name of the groupleader. Achim From owner-biophysics@net.bio.net Wed Jan 15 22:00:00 1997 Path: biosci!rutgers!gatech!csulb.edu!hammer.uoregon.edu!leto!news.ou.edu!news.onenet.net!news.ecn.uoknor.edu!paladin.american.edu!news.jhu.edu!NewsWatcher!user From: Neil.Clarke@qmail.bs.jhu.edu (Neil Clarke) Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Subject: Re: What is the SMALLEST DNA BINDING DOMAIN? Date: 15 Jan 1997 21:22:14 GMT Organization: Johns Hopkins Lines: 5 Message-ID: References: NNTP-Posting-Host: 128.220.123.53 Xref: biosci bionet.biophysics:2564 bionet.molbio.proteins:9746 bionet.info-theory:4563 bionet.molbio.evolution:5549 bionet.molbio.methds-reagnts:53527 Homeodomains are only 60 amino acids and most bind as monomers. -- Neil Clarke neil.clarke@qmail.bs.jhu.edu From owner-biophysics@net.bio.net Wed Jan 15 22:00:00 1997 Path: biosci!rutgers!gatech!www.nntp.primenet.com!nntp.primenet.com!feed1.news.erols.com!insync!news-feed.inet.tele.dk!news.algonet.se!eua.ericsson.se!erinews.ericsson.se!newsfeed.sunet.se!news00.sunet.se!sunic!mn6.swip.net!plug.news.pipex.net!pipex!oleane!pasteur.fr!univ-lyon1.fr!citi2.fr!news From: marc@u40024.citi2.fr (Marc Courregelongue) Newsgroups: bionet.biophysics Subject: Paris 6 Molecular Biophysics DEA Date: 16 Jan 1997 17:56:32 GMT Organization: GRPB Lines: 4 Message-ID: <5blq4g$6po@bisance.citi2.fr> NNTP-Posting-Host: u40018.citi2.fr Mime-Version: 1.0 Content-Type: Text/Plain; charset=ISO-8859-1 X-Newsreader: WinVN 0.99.5 The association of the dea de biophysique moleculaire of Paris 6 university is desesperately trying to bring up to date its new adress book If you are graduated from this dea please E mail me !!!!!!!!!! From owner-biophysics@net.bio.net Wed Jan 15 22:00:00 1997 Path: biosci!rutgers!gatech!csulb.edu!news.sgi.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!cpk-news-hub1.bbnplanet.com!feed1.news.erols.com!news.enteract.com!news.inetnebr.com!crcnews.unl.edu!unlinfo.unl.edu!mgriep From: mgriep@unlinfo.unl.edu (m griep) Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Subject: Re: What is the SMALLEST DNA BINDING DOMAIN? Followup-To: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Date: 16 Jan 1997 15:12:35 GMT Organization: University of Nebraska--Lincoln Lines: 35 Message-ID: <5blgh4$jts@crcnis3.unl.edu> References: <5bcck5$ho9@nntp3.u.washington.edu> <5bcds7$2gg10@ncisun1-nf0.ncifcrf.gov> <5blg10$jtj@crcnis3.unl.edu> NNTP-Posting-Host: unlinfo2.unl.edu X-Newsreader: TIN [version 1.2 PL2] Xref: biosci bionet.biophysics:2567 bionet.molbio.proteins:9749 bionet.info-theory:4566 bionet.molbio.evolution:5553 bionet.molbio.methds-reagnts:53535 m griep (mgriep@unlinfo.unl.edu) wrote: : Tom Schneider (toms@ncifcrf.gov) wrote: : : Angeline Kantola (kantola@u.washington.edu) wrote: : : : C2H2 zinc fingers are everywhere (there are other kinds of zinc fingers as : : : well), so finding information about them should be easy. Mia Schmiedeskamp : : : and Rachel Klevit wrote a review of zinc fingers a few years back, which : : : is probably a good place to start. : : Thanks for the tip. I could not locate this in medline entrez. : : Schmiedeskamp does appear but is the wrong topic. R. Klevit wrote a number : : of papers on the subject. : : Tom Schneider : : National Cancer Institute : : Laboratory of Mathematical Biology : : Frederick, Maryland 21702-1201 : : toms@ncifcrf.gov : : http://www-lmmb.ncifcrf.gov/~toms/ Recent reviews of or including Zn fingers: Vallee & Auld, 1993, Acc. Chem. Res. 26, 543-551. Schmiedeskamp & Klevit, 1994, Curr. Opinion Struct. Biol. 4, 28-35. Klug & Schwabe, 1995, FASEB J. 9, 597-604. : -- : Mark Griep (mgriep@unlinfo.unl.edu) : Department of Chemistry, University of Nebraska-Lincoln : Phone: 402-472-3429 FAX: 402-472-9402 -- Mark Griep (mgriep@unlinfo.unl.edu) Department of Chemistry, University of Nebraska-Lincoln Phone: 402-472-3429 FAX: 402-472-9402 From owner-biophysics@net.bio.net Wed Jan 15 22:00:00 1997 Path: biosci!rutgers!news.sgi.com!news.sprintlink.net!news-peer.sprintlink.net!howland.erols.net!worldnet.att.net!feed1.news.erols.com!news.enteract.com!news.inetnebr.com!crcnews.unl.edu!unlinfo.unl.edu!mgriep From: mgriep@unlinfo.unl.edu (m griep) Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.info-theory,bionet.molbio.evolution,bionet.molbio.methds-reagnts Subject: Re: What is the SMALLEST DNA BINDING DOMAIN? Date: 16 Jan 1997 15:04:00 GMT Organization: University of Nebraska--Lincoln Lines: 23 Message-ID: <5blg10$jtj@crcnis3.unl.edu> References: <5bcck5$ho9@nntp3.u.washington.edu> <5bcds7$2gg10@ncisun1-nf0.ncifcrf.gov> NNTP-Posting-Host: unlinfo2.unl.edu Vallee & Auld, 1993, Acc. Chem. Res. 26, 543-551. Schmiedeskamp & Klevit, 1994, Curr. Opinion Struct. Biol. 4, 28-35. Klug & Schwabe, 1995, FASEB J. 9, 597-604. X-Newsreader: TIN [version 1.2 PL2] Xref: biosci bionet.biophysics:2566 bionet.molbio.proteins:9748 bionet.info-theory:4565 bionet.molbio.evolution:5552 bionet.molbio.methds-reagnts:53534 Tom Schneider (toms@ncifcrf.gov) wrote: : Angeline Kantola (kantola@u.washington.edu) wrote: : : C2H2 zinc fingers are everywhere (there are other kinds of zinc fingers as : : well), so finding information about them should be easy. Mia Schmiedeskamp : : and Rachel Klevit wrote a review of zinc fingers a few years back, which : : is probably a good place to start. : Thanks for the tip. I could not locate this in medline entrez. : Schmiedeskamp does appear but is the wrong topic. R. Klevit wrote a number : of papers on the subject. : Tom Schneider : National Cancer Institute : Laboratory of Mathematical Biology : Frederick, Maryland 21702-1201 : toms@ncifcrf.gov : http://www-lmmb.ncifcrf.gov/~toms/ -- Mark Griep (mgriep@unlinfo.unl.edu) Department of Chemistry, University of Nebraska-Lincoln Phone: 402-472-3429 FAX: 402-472-9402 From owner-biophysics@net.bio.net Thu Jan 16 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Stephanie Dobler Newsgroups: bionet.biophysics Subject: ANNOUNCE: Supercomputing Techniques Workshop Date: 17 Jan 1997 00:36:50 -0800 Organization: Pittsburgh Supercomputing Center Lines: 171 Sender: daemon@net.bio.net Distribution: world Message-ID: <32DCE9AF.4EFB@psc.edu> Reply-To: dobler@psc.edu NNTP-Posting-Host: net.bio.net Pittsburgh Supercomputing Center Supercomputing Techniques: CRAY J90/C90 March 24-27, 1997 -------------------------------------------------------------------------------- REGISTRATION DEADLINE: March 3, 1997 -------------------------------------------------------------------------------- PURPOSE: The purpose of this three and a half day workshop is to introduce participants to computing on the CRAY J90/C90. Participants will gain experience in running jobs on the J90/C90, optimizing code, parallel processing, and on the use of graphics software libraries and packages. AGENDA: The first day of this workshop is designed to introduce participants to the J90/C90 platform. During this time, participants will become familiar with PSC's supercomputing environment, the UNICOS operating system, CRAY compiling, job submission, troubleshooting, and debugging. The last three days of this workshop are designed for users who are already familiar with running jobs on the CRAY J90/C90 and who are interested in more advanced topics: * The Code Optimization portion will cover performance monitoring techniques and strategies to improve the performance of one's code. * The Parallel Processing portion concentrates on the mechanics of parallel computing on the J90/C90 systems. * The final day will explore graphics and visualization topics ranging from a general overview of graphics packages available at PSC to a more advanced discussion of animation and 3-D graphics. To ensure that participants receive quality training, our workshops incorporate both lectures and extensive hands-on lab sessions. Programming exercises are carefully designed to reinforce concepts and techniques taught in class. Our instructors have strong scientific and technical backgrounds and are available for individual consultation during lab sessions. In addition to the lab exercises, lab time will also be available for participants to optimize their own code with the help and supervision of our instructors. Participants are encouraged to bring their code, but not required. ==> A working knowledge of FORTRAN or C and UNIX are required. REGISTRATION FEES: Admission to this training workshop is free to the United States academic community. Interested corporate and government applicants, as well as applicants from academic institutions outside the United States should contact Kathi Burgard at (412) 268-5131 for information on attendance fees. HOUSING AND TRAVEL: Housing and travel are the responsibility of participants, but we will provide information on local hotels at your request. Group rates for local hotels are available on a first-come, first-served basis. REGISTRATION: To register for Supercomputing Techniques: CRAY J90/C90, please complete and return the application form below by March 3, to: Workshop Application Committee ATTN: Kathi Burgard Pittsburgh Supercomputing Center 4400 Fifth Avenue, Pittsburgh, PA 15213. You may also apply for this workshop by sending requested information via electronic mail to workshop@psc.edu or via fax to (412/268-5832). All applicants will be notified of acceptance during the week of March 3, 1997. For additional online information, including a preliminary agenda, please visit the workshop's homepage: http://www.psc.edu/training/J90_Mar_97/welcome.html =============================================================================== Name: Please circle one: Ms. Mr. Dr. Prof. Department: Univ/Ind/Gov Affiliation: Address: Telephone: W ( ) H( ) Electronic Mail Address: Social Security Number: Citizenship: Are you a PSC user (yes/no)? If yes, please give your PSC username: Academic Standing (please check one): F - Faculty UG - Undergraduate I - Industrial PD - Postdoctorate UR - University Research Staff GV - Government GS - Graduate Student UN - University Non-Research Staff O - Other Please explain why you are interested in attending this workshop and what you hope to gain from it. Briefly describe your computing background (scalar, vector, and parallel programming experience; platforms; languages) and research interests. Please indicate the workshop for which you are applying: The following information is OPTIONAL and is for statistical reporting only. Ethnic origin: ___African American ___Hispanic ___Asian or Pacific Islander ___Caucasian ___American Indian or Alaskan Native ___Other (please specify) Sex: ___Male ___Female All applicants will be notified of acceptance during the week of March 3. From owner-biophysics@net.bio.net Thu Jan 16 22:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.biophysics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Jan 1997 03:44:00 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199701171000.CAA28876@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-biophysics@net.bio.net Thu Jan 16 22:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!newsserver.jvnc.net!ganglia.bms.com!not-for-mail From: Dan Brown Newsgroups: bionet.biophysics Subject: thermal output of actively fermenting bacteria Date: Fri, 17 Jan 1997 16:51:28 +0000 Organization: Bristol-Myers Squibb PRI Lines: 5 Message-ID: <32DFAE10.75B@usccmail.bms.com> Reply-To: dbrown3@usccmail.bms.com NNTP-Posting-Host: 301cmac.wf.bms.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Does anyone know of any references that address the heat output per cell of a bacterial culture in log-phase growth at 37 degrees C in liquid media? dbrown3@usccmail.bms.com From owner-biophysics@net.bio.net Fri Jan 17 22:00:00 1997 Path: biosci!daresbury!nntp-trd.UNINETT.no!hermod.uio.no!nntp.uio.no!newsfeeds.sol.net!newspump.sol.net!mindspring!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!uunet!in3.uu.net!165.87.194.248!news-m01.ny.us.ibm.net!news-s01.ny.us.ibm.net!not-for-mail From: "Ahmed Lazrak" Newsgroups: bionet.biophysics Subject: resume: electrophysiology and biophysics Date: 18 Jan 1997 13:46:36 GMT Organization: home Lines: 164 Message-ID: <01bc0546$52584f20$a8f148a6@default> NNTP-Posting-Host: slip166-72-241-168.tn.us.ibm.net Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: base64 X-Newsreader: Microsoft Internet News 4.70.1160 QWhtZWQgTGF6cmFrDQo0MTEgV2VzdCBWYW5kZXJiaWx0IERyaXZlDQpPYWsgUmlkZ2UsIFROIDM3 ODMwDQoNCm9yDQoNCkFobWVkIExhenJhayBQaC5ELg0KT1JOTCBFTUYgQmlvZWZmZWN0cyBSZXNl YXJjaCBQcm9ncmFtICANCk9hayBSaWRnZSBOYXRpb25hbCBMYWJvcmF0b3J5DQpQTyBCb3ggMjAw OCwgTVMtNjEyNQ0KT2FrIFJpZGdlLCBUTiAzNzgzMS02MTI1DQpUZWw6IDQyMy0yNDEtNDM1OA0K RmF4OiA0MjMtNTc2LTQ0MDcNCg0KQWhtZWQuTGF6cmFrQElibS5uZXQNCkJuT