From owner-biophysics@net.bio.net Wed Apr 02 23:00:00 1997 Path: biosci!daresbury!usenet From: blf@indigo7.dl.ac.uk (L.M.Murphy) Newsgroups: bionet.biophysics Subject: Ph. D studentship in molecular biophysics Date: 03 Apr 1997 11:42:35 GMT Organization: Daresbury Laboratory, Warrington, UK Lines: 43 Distribution: bionet Message-ID: References: <5d0jal$evq@news.mty.itesm.mx> NNTP-Posting-Host: indigo7.dl.ac.uk In-reply-to: al764830@academ10.mty.itesm.mx's message of 1 Feb 1997 23:24:05 GMT Ph.D Studentship 6500 UKpounds/pa in Synchrotron Radiation and Metalloproteins. Applications are invited for a studentship associated with an interdisciplinary research programme on the structure/ function of metalloproteins. The research work would involve the use of state of the art physical techniques (e.g., X-ray Crystallography, X-ray Scattering & XAFS) and the modern methods of biotechnology. The studentship is collaboratively funded by the John Innes centre and the De Montfort University and is aimed at understanding the structure/function relationships of proteins involved in denitrification and nitrogen fixation. We have access to the excellent X-ray and computing facilities at the CCLRC Daresbury Laboratory. Applications enclosing a CV, names and addresses of two referees should be sent to Professor S. Samar Hasnain, School Of Applied Sciences, De Montfort University at Leicester, The Gateway, Leicester LE1 9BH or at CCLRC Daresbury Laboratory, Warrington WA4 4AD. The closing date is 20 April 1997. -- Yours Lorrie Murphy Station Manager 8.1 CCLRC Daresbury Laboratory, Daresbury, Warrington, WA4 4AD UK Tel: 01925 603 625 Fax: 01925 603 124 E-mail: L.M.Murphy@dl.ac.uk From owner-biophysics@net.bio.net Wed Apr 02 23:00:00 1997 Path: biosci!HAVERFORD.EDU!jdepaula From: jdepaula@HAVERFORD.EDU (Julio de Paula) Newsgroups: bionet.biophysics Subject: Laboratory coordinator position at Haverford College Date: 3 Apr 1997 12:43:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net TEACHING LABORATORY SUPERVISOR AT HAVERFORD COLLEGE (DEPARTMENT OF CHEMISTRY) - Full time for 9 month academic year, renewable for subsequent academic years. Responsibilities include preparation of solutions, materials, and equipment for general chemistry and organic chemistry course laboratories, along with supervision of student help for these duties, and some supervisory work and grading for the laboratory sections. Instrumentation used in the course laboratories includes UV/vis, IR, and NMR spectrometers, and gas chromatography. Experience with some or all of these techniques is desirable. MA/MS (or PhD) in Chemistry required. Send resume, with references, to: Department of Chemistry, attn. Terry Timberlake, Haverford College, Haverford, PA 19041. Applications submitted by electronic mail will not be accepted. EOE/AA From owner-biophysics@net.bio.net Wed Apr 02 23:00:00 1997 Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!azure.xara.net!xara.net!rill.news.pipex.net!pipex!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsxfer3.itd.umich.edu!howland.erols.net!torn!newshost.uwo.ca!usenet From: David O'Neill Newsgroups: bionet.biophysics,bionet.general,bionet.molec-model,uwo.biochem Subject: Q: Blood Flow Date: Thu, 03 Apr 1997 18:16:17 -0500 Organization: University of Western Ontario, Ca Lines: 8 Message-ID: <33443A01.7635@julian.uwo.ca> NNTP-Posting-Host: ts3-1.slip.uwo.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Authenticated: doneill2@ts3-1.slip.uwo.ca X-Mailer: Mozilla 3.0 (Win95; I) Xref: biosci bionet.biophysics:2795 bionet.general:26302 bionet.molec-model:1469 Hi there, I'm looking for an experimetal model to alter blood flow in DISUSED rodent skeletal muscle (hindlimb). At present I'm using a few models: hindlimb suspension, ttx neurotoxin induced disuse (sympathectomy) and prazosin (vasodilator). I'm looking for another model with which to compare these. Any ideas? Thanks in adavance, Dave. From owner-biophysics@net.bio.net Wed Apr 02 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!www.nntp.primenet.com!nntp.primenet.com!newsfeed.nacamar.de!worldnet.att.net!howland.erols.net!torn!newshost.uwo.ca!usenet From: David O'Neill Newsgroups: bionet.biophysics,bionet.general,bionet.molec-model,uwo.biochem Subject: Q: Necrosis vs. Apoptosis Date: Thu, 03 Apr 1997 18:27:58 -0500 Organization: University of Western Ontario, Ca Lines: 13 Message-ID: <33443CFE.64D8@julian.uwo.ca> NNTP-Posting-Host: ts3-1.slip.uwo.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Authenticated: doneill2@ts3-1.slip.uwo.ca X-Mailer: Mozilla 3.0 (Win95; I) Xref: biosci bionet.biophysics:2796 bionet.general:26304 bionet.molec-model:1470 Hi again, I'm trying to differentiate between necrotic endothelial cells and apoptotic endothelial cells. Other than morphological analysis of blebbing etc. are there any immunohistochemical markers capable of this distinction. For instance, a marker for two differing types of DNA fragmentation? Due to the loss of membrane integrity during necrosis, I wonder if it is physically possible to identify these cells at all. Diffuse counterstaining (of necrotic cells) against an apoptotic marker won't help me as I need to identify (and count) capillaries undergoing necrosis. Any suggestions are appreciated. Have a great day! Thanks, Dave. From owner-biophysics@net.bio.net Thu Apr 03 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!news-relay.ncren.net!newz.oit.unc.edu!mac295a6013.med.unc.edu!user From: jek@med.unc.edu (Jackie E. Kylander) Newsgroups: bionet.biophysics,bionet.general,bionet.molec-model,uwo.biochem Subject: Re: Q: Necrosis vs. Apoptosis Date: Fri, 04 Apr 1997 08:35:39 -0500 Organization: UNC-Chapel Hill Lines: 29 Message-ID: References: <33443CFE.64D8@julian.uwo.ca> NNTP-Posting-Host: mac295a6013.med.unc.edu Xref: biosci bionet.biophysics:2797 bionet.general:26312 bionet.molec-model:1472 In article <33443CFE.64D8@julian.uwo.ca>, David O'Neill wrote: >Hi again, >I'm trying to differentiate between necrotic endothelial cells and >apoptotic endothelial cells. Other than morphological analysis of >blebbing etc. are there any immunohistochemical markers capable of this >distinction. For instance, a marker for two differing types of DNA >fragmentation? Due to the loss of membrane integrity during necrosis, I >wonder if it is physically possible to identify these cells at all. >Diffuse counterstaining (of necrotic cells) against an apoptotic marker >won't help me as I need to identify (and count) capillaries undergoing >necrosis. >Any suggestions are appreciated. Have a great day! >Thanks, >Dave. Dave, I have no suggestions for you only the best of luck. I've tried several of the commercial "apoptosis" detection kits but ALL have overestimated the number of apoptotic cells in our model (tracheal injury and repair). Each kit has given way over 50% detection--a number that cannot be backed up by any other method (PC10 staining, morphology, BRdU, cell nucleus counts, etc, etc.) we suspect a problem with fixation/embedment but cannot seem to pin it down. -- Jackie E. Kylander A/OSB From owner-biophysics@net.bio.net Thu Apr 03 23:00:00 1997 Path: biosci!BUPHYC.BU.EDU!BC From: BC@BUPHYC.BU.EDU Newsgroups: bionet.biophysics Subject: nitella Date: 4 Apr 1997 07:36:16 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IHAZ33BEQQ0000N7@buphyc.bu.edu> NNTP-Posting-Host: net.bio.net Does anybody know of a source for nitella, the alga used in actin studies ? Thank you. B Chasan, Physics Dept, Boston U. From owner-biophysics@net.bio.net Thu Apr 03 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.maxwell.syr.edu!ix.netcom.com!news From: davidis@ix.netcom.com(David Isaac Sternberg) Newsgroups: bionet.biophysics Subject: Problems with Flurorescent Spectroscopy Date: 4 Apr 1997 23:08:14 GMT Organization: Netcom Lines: 11 Message-ID: <5i41ku$qu3@sjx-ixn5.ix.netcom.com> NNTP-Posting-Host: nyc-ny12-30.ix.netcom.com X-NETCOM-Date: Fri Apr 04 3:08:14 PM PST 1997 Hi there, I am a biologist without a tremendous math or chemistry background trying to do some fluoresent spectroscopy. I am trying to measure the decay of fluoresent anisotropy after shooting plane polarized light at an enzyme bound to a planar fluorescent probe. I would really like a book that will start off very easy with the math but will go into the type of fluorescent spectroscopy I need to know. If anyone has any tips about how I should educate myself, I would gladly appreciate them. David E-mail: davidis@ix.netcom.com From owner-biophysics@net.bio.net Sat Apr 05 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!news.icm.edu.pl!newsfeed.nacamar.de!worldnet.att.net!howland.erols.net!news-peer.sprintlink.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!ix.netcom.com!news From: davidis@ix.netcom.com(David Isaac Sternberg) Newsgroups: bionet.biophysics Subject: Protein Crystals in space Date: 6 Apr 1997 04:23:53 GMT Organization: Netcom Lines: 22 Message-ID: <5i78gp$out@sjx-ixn5.ix.netcom.com> NNTP-Posting-Host: nyc-ny13-16.ix.netcom.com X-NETCOM-Date: Sat Apr 05 8:23:53 PM PST 1997 Hi there, As many of you well know, the space shuttle Columbia is currently in orbit. One of the experiments that the astronauts will carry out will be the crystallization of protein crystals in space. In their last statement, NASA stated that that it hopes that protein crystallization in space will yield important information that will enable them to better design drugs against a variety of pathogens. I, however, do not understand what effect they expect a microgravity environment to have on the growth of protein crystalls. I never even knew gravity was a consideration in that type of work! I also don't understand why they think more crystal structures will help scientists design drugs, as I am aware that so far rational drug design has met with a very limited amount of excess even with a whole slew of protein structures solved while bound to whole array of inhibitors. Can anyone explain the rationale of the NASA experiments. I am certian that if they are funding this work there has to be a good reason for it. I just don't see it. Sincerely, David S. E-mail: davidis@ix.netcom.com From owner-biophysics@net.bio.net Sun Apr 06 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.maxwell.syr.edu!news.emi.com!euclid.wadsworth.org!tivol From: tivol@news.wadsworth.org (William Tivol) Newsgroups: bionet.biophysics Subject: Re: Protein Crystals in space Date: 7 Apr 1997 18:53:30 GMT Organization: Wadsworth Center, NY Health Dept. Lines: 39 Message-ID: <5ibfra$8ca@euclid.wadsworth.org> References: <5i78gp$out@sjx-ixn5.ix.netcom.com> NNTP-Posting-Host: alcor.wadsworth.org X-Newsreader: TIN [version 1.2 PL2] David Isaac Sternberg (davidis@ix.netcom.com) wrote: : As many of you well know, the space shuttle Columbia is currently : in orbit. One of the experiments that the astronauts will carry out : will be the crystallization of protein crystals in space. In their : last statement, NASA stated that that it hopes that protein : crystallization in space will yield important information that will : enable them to better design drugs against a variety of pathogens. I, : however, do not understand what effect they expect a microgravity : environment to have on the growth of protein crystalls. I never even : knew gravity was a consideration in that type of work! Very small forces affect the growth of protein crystals because the free energy differences between the crystals and non-crystalline forms are small. In order to do x-ray diffraction studies, very regular cry- stals of relatively large size are best. Growing these in a zero-g en- vironment leads to larger and better-quality crystals. There have been some pictures published of the space-grown crystals which you can compare to those grown in earth-bound labs--the differences are readily apparent. : I also don't : understand why they think more crystal structures will help scientists : design drugs, as I am aware that so far rational drug design has met : with a very limited amount of excess even with a whole slew of protein : structures solved while bound to whole array of inhibitors. In some cases, the proteins involved may not have been able to be adequately crystallized. The specific protein structure is much more important than how many total structures have been determined. Even a limited success could be very important (e.g., a cure for AIDS). : Can anyone explain the rationale of the NASA experiments. I am certian : that if they are funding this work there has to be a good reason for : it. I just don't see it. Once the shuttle is going up, inclusion of a protein crystalliza- tion experiment is low-cost, takes up little space and requires minimal attention. Yours, Bill Tivol From owner-biophysics@net.bio.net Sun Apr 06 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!news-xfer.netaxs.com!cronkite.ocis.temple.edu!astro.temple.edu!not-for-mail From: driska@astro.temple.edu (Stephen P. Driska PhD) Newsgroups: bionet.biophysics Subject: Re: Protein Crystals in space Date: 7 Apr 1997 21:06:25 GMT Organization: Temple University, Academic Computer Services Lines: 39 Message-ID: <5ibnkh$b27@cronkite.ocis.temple.edu> References: <5i78gp$out@sjx-ixn5.ix.netcom.com> NNTP-Posting-Host: astro.ocis.temple.edu NNTP-Posting-User: 536870664 X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Guess I didn't post my article right. Will try again.... David Isaac Sternberg (davidis@ix.netcom.com) wrote: [about NASA experiments on space shuttle] I, : however, do not understand what effect they expect a microgravity : environment to have on the growth of protein crystalls. I never even : knew gravity was a consideration in that type of work! I also don't On earth, when areas of a fluid have different densities, an equal size volume of the denser fluid is heavier so it sinks, setting up things called convection currents. For example, when the surface of a lake cools off in the fall, that water sinks. You can observe this in a cup of hot water cooling off. As I understand it, in zero gravity (never been there), areas of a fluid which are denser don't sink, and convection currents are not set up, so there is no mixing. I assumed this is relevant to protein crystallinzation. It is the rationale given for doing certain electrophoresis experiments on the space shuttle, and it makes sense. Is this the mission on which they want to study the process of fire? It sounds pretty fundamental. If hot expanding gases are less dense, will they go "up"? without gravity, who knows? It seems that fire could act a whole lot differently in zero gravity. Hope I can post this right this time (new hardware/software at work).... Steve -- Steve Driska, Physiology Department, Temple University Medical School Philadelphia, PA 19140 USA (215) 707-3283 driska@astro.ocis.temple.edu From owner-biophysics@net.bio.net Sun Apr 06 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!news.icm.edu.pl!news.apfel.de!newsfeed.nacamar.de!news-peer.gsl.net!howland.erols.net!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!news-xfer.netaxs.com!cronkite.ocis.temple.edu!astro.temple.edu!not-for-mail From: driska@astro.temple.edu (Stephen P. Driska PhD) Newsgroups: bionet.biophysics Subject: Re: Protein Crystals in space Date: 7 Apr 1997 20:53:02 GMT Organization: Temple University, Academic Computer Services Lines: 29 Message-ID: <5ibmre$b27@cronkite.ocis.temple.edu> References: <5i78gp$out@sjx-ixn5.ix.netcom.com> NNTP-Posting-Host: astro.ocis.temple.edu NNTP-Posting-User: 536870664 X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] David Isaac Sternberg (davidis@ix.netcom.com) wrote: : Hi there, : : As many of you well know, the space shuttle Columbia is currently : in orbit. One of the experiments that the astronauts will carry out : will be the crystallization of protein crystals in space. In their : last statement, NASA stated that that it hopes that protein : crystallization in space will yield important information that will : enable them to better design drugs against a variety of pathogens. I, : however, do not understand what effect they expect a microgravity : environment to have on the growth of protein crystalls. I never even : knew gravity was a consideration in that type of work! I also don't : understand why they think more crystal structures will help scientists : design drugs, as I am aware that so far rational drug design has met : with a very limited amount of excess even with a whole slew of protein : structures solved while bound to whole array of inhibitors. : Can anyone explain the rationale of the NASA experiments. I am certian : that if they are funding this work there has to be a good reason for : it. I just don't see it. : : Sincerely, : David S. : E-mail: davidis@ix.netcom.com -- Steve Driska, Physiology Department, Temple University Medical School Philadelphia, PA 19140 USA (215) 707-3283 driska@astro.ocis.temple.edu From owner-biophysics@net.bio.net Mon Apr 07 23:00:00 1997 Path: biosci!agate!spool.mu.edu!uwm.edu!news.cse.psu.edu!news3.cac.psu.edu!howland.erols.net!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!news.maxwell.syr.edu!nntp.uio.no!uninett.no!news.uni-c.dk!news-inn.uni-c.dk!news.uni-c.dk!balder.adm.ku.dk!not-for-mail From: Claus Lundegaard Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.biophysics Subject: Meeting on Enzyme Families in Nucleotide Metabolism, new deadline Date: Fri, 04 Apr 1997 13:38:18 +3500 Organization: Copenhagen University Lines: 19 Message-ID: <3349AF9A.167E@mermaid.molbio.ku.dk> NNTP-Posting-Host: cef.molbio.ku.dk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (X11; I; IRIX 6.2 IP22) Xref: biosci bionet.biophysics:2806 bionet.molbio.proteins:10437 Please see new deadline for attending this meeting in copenhagen: MAY 1 For further details see: http://cef.molbio.ku.dk/moede.html -- ___________________________________________________________________________ ******************************************************* Claus Lundegaard Center For Enzyme Research Soelvgade 83 H 1307 Koebenhavn K phone: (+45) 35 32 20 18 fax: (+45) 35 32 20 40 e-mail: mailto:lundegaard@mermaid.molbio.ku.dk www: http://cef.molbio.ku.dk/~lunde/Claus.html ******************************************************* From owner-biophysics@net.bio.net Mon Apr 07 23:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!howland.erols.net!cam-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!ais.net!uunet!in2.uu.net!206.250.118.17!nntp.earthlink.net!usenet From: "Alex Chiu" Newsgroups: bionet.biophysics Subject: Eternal LIfe Device Date: 8 Apr 1997 19:59:24 GMT Organization: Earthlink Network, Inc. Lines: 41 Message-ID: <01bc4447$a08886c0$3bc30b26@itjfvkli> NNTP-Posting-Host: ip59.san-francisco2.ca.pub-ip.psi.net X-Newsreader: Microsoft Internet News 4.70.1155 NEW INVENTION ALLOWS HUMANS TO LIVE FOREVER If you disagree or disbelieve the content enclosed, please don't get upset. I am just publicizing my theory and my discovery based on my belief and observation. If you believe my report, or interested, please see my site http://home.earthlink.net/~alexycchiu Press Release ACCORDING TO ALEX CHIU, PEOPLE CAN STAY YOUNG AND LIVE FOREVER BY USING HIS NEW INVENTION 'THE ETERNAL LIFE RINGS' OR 'THE ETERNAL LIFE FOOT BRACES'. The Eternal Life Rings are rings to be worn on both small fingers of a user during sleep. The Eternal Life Foot Braces are to be worn on all fingers of both feet of a user during sleep. Both devices consist simply magnets and braces which hold magnets onto the fingers of the user. Mr. Alex Chiu claims that the magnetic devices, the finger rings or the foot braces, both are believed to allow the user to stay young physically forever or to become younger! The inventor explained that the magnetic pole on the right small finger or on the right foot is opposite to the poles of the magnets on the left small finger or of the left foot. With a different pole on each side of the human body, blood circulation and electric current of the body are propelled. The speeded up blood circulation and electric current generate faster metabolism in order to fight aging process. The mentioned devices invented by Alex Chiu are believed to be able to alter aging or to keep the users young physically as long as the users wear them every night during sleep. The devices also cure many handicapnesses and deseases. Mr.Alex Chiu is an inventor who is mainly specialized in effects on human health by magnetic forces. For interested party, please email alexycchiu@earthlink.net Check out my web site http://home.earthlink.net/~alexycchiu tel 415 585 3825 pobox 16547 San Francisco CA 94116 From owner-biophysics@net.bio.net Mon Apr 07 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!news-xfer.netaxs.com!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!spring.edu.tw!news.nctu.edu.tw!news.cc.nctu.edu.tw!news.ncu.edu.tw!news From: "cjc" Newsgroups: bionet.biophysics Subject: About Fractal Question Date: 8 Apr 1997 16:17:18 GMT Organization: ¤¤¥¡¤j¾Ç¤g¤ì©Ò¤ô¸ê·½²Õ Lines: 14 Message-ID: <01bc451a$390c39a0$d13c738c@cjc.NCU.EDU.TW> NNTP-Posting-Host: @140.115.60.209 X-Newsreader: Microsoft Internet News 4.70.1155 Dear Sir: I am a graduate student who major in civil engineering in Taiwan, R.O.C. Right now I am investigating about the effect of the fractals characteristics of river networks to the shape of the hydrograph. I come across some problem. I need your help. If you do research or know about the effect of fractals characteristics of river networks to the peak flow, the time to peak, the time base of the hydrograph, could you provide relative information for me ? I appreciate it. Reply my e-mail: s3252070@cc.ncu.edu.tw From owner-biophysics@net.bio.net Mon Apr 07 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!news.maxwell.syr.edu!news.apfel.de!news-fra1.dfn.de!news-ge.switch.ch!news-zh.switch.ch!rzunews.unizh.ch!newsadm From: Rolf Kocherhans Newsgroups: bionet.biophysics Subject: Free practical programs for molecular biologists !!! Date: Tue, 08 Apr 1997 15:20:32 +0100 Organization: University of Zurich Lines: 49 Message-ID: <334A542F.4042@vetvir.unizh.ch> Reply-To: rolfk@vetvir.unizh.ch NNTP-Posting-Host: 130.60.22.38 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Macintosh; I; PPC) I wrote a few practical and simple to use programs which facilitate your daily work in the lab such as predicting the size of DNA fragments after digestion (graphical) with restriction enzymes. I would like to make these programs accessible to a broad user group by the Internet. All programs have been tested on MacOS and Windows95. My programs are accessible over the WWW and made functional using Netscape 2.x or Internet Explorer 2.x or higher in association with a free plugin which you have to download and install first. This is what you do: - Download the Roadster plugin http://www.unizh.ch/vetvir/plugin.html) install it on your computer. - Then connect to: http://www.unizh.ch/vetvir/programs.html That's it !! These are my programs which make your live as a molecular biologist easier ! Find here a few more examples or my programs: a. Digest Preview: Enter the size(s) of your DNA fragment(s) and see their migration pattern in a virtual gel in comparison to a 1 kb ladder. b. Adapter Design: Helps to create in frame adapters in order to link incompatible DNA ends together. c. Dilution Calculator: Does all the calculations when you have to make up solutions There are many other programs such as Oligo Tm, Compatible ends etc. Please have a look, comments are welcome! Have fun Rolf Kocherhans mailto:rolfk@vetvir.unizh.ch From owner-biophysics@net.bio.net Mon Apr 07 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: ucbt143@ucl.ac.uk (Prof Mike Evans) Newsgroups: bionet.biophysics Subject: Postdoc position biophysics of photosynthesis. London UK Date: 8 Apr 1997 12:03:53 +0100 Lines: 29 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5id8mp$69e@mserv1.dl.ac.uk> X-Sender: ucbt143@pop-server.bcc.ac.uk (Unverified) Original-To: biophys@dl.ac.uk Post-doc position in photosynthesis London UK A position is available for a post doctoral research associate in the=20 Photosynthesis Research Group at University College London/Queen Mary and=20 Westfield College to work on the spectroscopic analysis of the quinone (A1)= =20 binding site in photosystem 1. The programme,, under the direction of Mike= =20 Evans (UCL) Pete Heathcote & Steve Rigby (QMW), will utilise EPR, ENDOR,=20 pulsed EPR and FTIR to analyse quinone protein interactions in WT and mutant= =20 PS1 reaction centres. A background in biophysics, biochemistry or physical= =20 chemistry, with experience of advanced spectroscopic techniques would be an= =20 advantage.=20 The post is supported by BBSRC with salary on the 1A scale =A316866- 20107 Further information can be obtained from Prof. M.C.W. Evans, Dept. of=20 Biology. Darwin Building, UCL, Gower St, London WC1E 6BT, UK.=20 +44-(0)171-380-7312, mike.evans@ucl.ac.uk, to whom applications including cv= =20 and names of 2 referees should be sent. Prof. M.C.W. Evans e-mail mike.evans@ucl.ac.uk Dept of Biology University College London phone +44 (0)171-380-7312 Gower St fax +44 (0)171-380-7096 London WC1E 6BT UK. From owner-biophysics@net.bio.net Wed Apr 09 23:00:00 1997 Path: biosci!biochem.umass.edu!bscott From: bscott@biochem.umass.edu (Brian Scott) Newsgroups: bionet.biophysics Subject: Free molecular visualization workshops for profs Date: 9 Apr 1997 17:44:22 -0700 Organization: University of Massachusetts Lines: 53 Sender: daemon@net.bio.net Distribution: world Message-ID: <334C37A6.424F@biochem.umass.edu> Reply-To: bscott@biochem.umass.edu NNTP-Posting-Host: net.bio.net Free Workshops on Molecular Visualization for Undergraduate Bioscience Teaching Amherst MA -- Summer, 1997-8 Understanding the three-dimensional structures of proteins, DNA, RNA, and their interactions is difficult from flat pictures, yet grasping structure is important to understanding function. Free software is now available which displays attention-grabbing 3D animated images of biological molecules in depth-cued spacefilling color. RasMol (http://www.umass.edu/microbio/rasmol), which works on Windows or Macs, encourages self-directed exploration. Chime (http://www.umass.edu/microbio/chime) allows annotated preset views of molecules to be delivered as web tutorials. Free, hands-on workshops will be held this summer at the University of Massachusetts in Amherst to prepare college faculty with no prior experience to use molecular visualization in their classes. Participants will travel to the Amherst campus on three separate days (two this summer, one the following summer), and are responsible for their own travel expenses (and therefore are expected primarily from the Northeastern USA). Overnight accomodations will be provided free to those needing them (based on distance traveled). Participants will be expected to incorporate molecular visualization into their teaching, and to mentor two faculty colleagues at their home institution. The workshops will be led by Eric Martz, a Professor in the Department of Microbiology. Martz has innovated molecular structure tutorials which are in use throughout the USA and in dozens of other countries. The web site he created (see above) was visited by 40,000 people in 1996. 1997 dates are June 17 and 30 (workshop A), or June 19 and July 2 (workshop B). Workshops A and B also meet for one day in late June 1998. To obtain more detailed information and a REGISTRATION FORM, visit http://www.umass.edu/microbio/rasmol/workshop.htm or FAX a request to 413-545-1578, or email a request to emartz@microbio.umass.edu. Supported by the National Science Foundation (Undergraduate Faculty Enhancement, Division of Undergraduate Education) and the University of Massachusetts. /* - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Eric Martz, Professor (Immunology), Dept Microbiology, Univ Massachusetts, Amherst MA US 01003-5720 413-545-2325 FAX:545-1578. RasMol Home Page, http://www.umass.edu/microbio/rasmol Other web projects listed at http://www.umass.edu/microbio/rasmol/em-web.htm - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -*/ From owner-biophysics@net.bio.net Wed Apr 09 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!news-xfer.netaxs.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.maxwell.syr.edu!news.cis.ohio-state.edu!magnus.acs.ohio-state.edu!OH6_pc_115.acs.ohio-state.edu!zuo.4 From: zuo.4@postbox.acs.ohio-state.edu (li zuo) Newsgroups: bionet.biophysics Subject: Re: Protein Crystals in space Date: Thu, 10 Apr 1997 01:01:13 GMT Organization: The Ohio State University Lines: 51 Message-ID: References: <5i78gp$out@sjx-ixn5.ix.netcom.com> <5ibnkh$b27@cronkite.ocis.temple.edu> NNTP-Posting-Host: oh13pc15.acs.ohio-state.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #1] In article <5ibnkh$b27@cronkite.ocis.temple.edu> driska@astro.temple.edu (Stephen P. Driska PhD) writes: >Path: >magnus.acs.ohio-state.edu!usenet.ins.cwru.edu!gatech!csulb.edu!hammer.uoregon.ed >u!cyclic.gsl.net!news.gsl.net!news.maxwell.syr.edu!europa.clark.net!newsfeeds.so >l.net!news-xfer.netaxs.com!cronkite.ocis.temple.edu!astro.temple.edu!not-for-mai >l >From: driska@astro.temple.edu (Stephen P. Driska PhD) >Newsgroups: bionet.biophysics >Subject: Re: Protein Crystals in space >Date: 7 Apr 1997 21:06:25 GMT >Organization: Temple University, Academic Computer Services >Lines: 39 >Message-ID: <5ibnkh$b27@cronkite.ocis.temple.edu> >References: <5i78gp$out@sjx-ixn5.ix.netcom.com> >NNTP-Posting-Host: astro.ocis.temple.edu >NNTP-Posting-User: 536870664 >X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] >Xref: magnus.acs.ohio-state.edu bionet.biophysics:2783 >Guess I didn't post my article right. Will try again.... >David Isaac Sternberg (davidis@ix.netcom.com) wrote: > [about NASA experiments on space shuttle] >I, >: however, do not understand what effect they expect a microgravity >: environment to have on the growth of protein crystalls. I never even >: knew gravity was a consideration in that type of work! I also don'tfw >is no mixing. I assumed this is relevant to protein crystallinzation. >It is the rationale given for doing certain electrophoresis experiments >on the space shuttle, and it makes sense. > Is this the mission on which they want to study the process of >fire? It sounds pretty fundamental. If hot expanding gases are less >dense, will they go "up"? without gravity, who knows? It seems that >fire could act a whole lot differently in zero gravity. > Hope I can post this right this time (new hardware/software at >work).... > Steve > >-- >Steve Driska, Physiology Department, Temple University Medical School >Philadelphia, PA 19140 USA (215) 707-3283 >driska@astro.ocis.temple.edu From owner-biophysics@net.bio.net Thu Apr 10 23:00:00 1997 Path: biosci!csb.ki.se!tony.wright From: tony.wright@csb.ki.se (Tony Wright) Newsgroups: bionet.biophysics Subject: Postdoc Positions in Gene Regulation Available Date: 11 Apr 1997 06:58:00 -0700 Organization: Karolinska Institute Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: <334E462D.11BD@cbt.ki.se> Reply-To: tony.wright@csb.ki.se NNTP-Posting-Host: net.bio.net Two postdoctoral positions are availble to take part in a multidisciplinary project to study mechanisms involved in gene regulation. Position 1. Biochemist with experience in purification of recombinant proteins expressed in E. coli and with an interest in protein structure and folding. You will investigate the structure of transcriptional activation and repression domains and how their structure is influenced by interaction with target proteins (see Biochemistry 35, 9584-93, 1996). Position 2. Molecular biologist with experience in yeast genetics. You will use genetic screens in yeast to identify proteins that are important for the function of mammalian transcriptional activation domains expressed in yeast (see Mol. Cell. Biol. 17, 934-45, 1997). The positions will be based at the Centre for Biotechnology, Dept. of Biosciences, Karolinska Institute and will involve close collaboration with the groups of Torleif H=E4rd at the adjacent Centre for Structural Biochemistry (+46-8-6089230, torleif.hard@cnt.ki.se) (pos.1) and Hans Ronne, University of Uppsala (+46-18-174239, hans.ronne@bmc.uu.se) (pos. 1 & 2). The positions are available for up to 2 years in the first instance.=20 Applications, including CV, relevant publications and two letters of recommendation should be sent by 1st May, 1997 to Associate Professor Anthony Wright, Karolinska Institute, Department of Biosciences at Novum, S-141 57 Huddinge, Sweden. Telephone: +46-8-6089159, Fax: +46-8-7745538, email: tony.wright@cbt.ki.se. From owner-biophysics@net.bio.net Sun Apr 13 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!arclight.uoregon.edu!newsfeed.direct.ca!news.maxwell.syr.edu!news.apfel.de!news-fra1.dfn.de!news-ge.switch.ch!news-zh.switch.ch!rzunews.unizh.ch!newsadm From: Rolf Kocherhans Newsgroups: bionet.biophysics Subject: Free practical programs for molecular biologists !!! Date: Mon, 14 Apr 1997 09:38:59 +0100 Organization: University of Zurich Lines: 49 Message-ID: <3351ED22.3A34@vetvir.unizh.ch> Reply-To: rolfk@vetvir.unizh.ch NNTP-Posting-Host: 130.60.22.38 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Macintosh; I; PPC) I wrote a few practical and simple to use programs which facilitate your daily work in the lab such as predicting the size of DNA fragments after digestion (graphical) with restriction enzymes. I would like to make these programs accessible to a broad user group by the Internet. All programs have been tested on MacOS and Windows95. My programs are accessible over the WWW and made functional using Netscape 2.x or Internet Explorer 2.x or higher in association with a free plugin which you have to download and install first. This is what you do: - Download the Roadster plugin http://www.unizh.ch/vetvir/plugin.html) install it on your computer. - Then connect to: http://www.unizh.ch/vetvir/programs.html That's it !! These are my programs which make your live as a molecular biologist easier ! Find here a few more examples or my programs: a. Digest Preview: Enter the size(s) of your DNA fragment(s) and see their migration pattern in a virtual gel in comparison to a 1 kb ladder. b. Adapter Design: Helps to create in frame adapters in order to link incompatible DNA ends together. c. Dilution Calculator: Does all the calculations when you have to make up solutions There are many other programs such as Oligo Tm, Compatible ends etc. Please have a look, comments are welcome! Have fun Rolf Kocherhans mailto:rolfk@vetvir.unizh.ch From owner-biophysics@net.bio.net Sun Apr 13 23:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!uwm.edu!newsfeeds.sol.net!hammer.uoregon.edu!news.uoregon.edu!marlin.ucsf.edu!overload.lbl.gov!news From: trey simmons Newsgroups: bionet.molbio.proteins,bionet.molecules.peptides,sci.chem.analytical,bionet.biophysics,bionet.molbio.methds-reagnts Subject: Re: Ion Exchange Chro/HPLC with 6M Guanidine or Some Detergent? Date: Mon, 14 Apr 1997 14:00:30 -0700 Organization: lawrence berkeley Lines: 55 Message-ID: <33529AEE.318A@csg.lbl.gov> References: <5frrbl$e16$1@snunews.snu.ac.kr> Reply-To: trey@csg.lbl.gov NNTP-Posting-Host: xlab1.als.lbl.gov Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.0b2 (WinNT; I) To: newera@plaza.snu.ac.kr X-Priority: 3 (Normal) Xref: biosci bionet.molbio.proteins:10475 bionet.molecules.peptides:745 sci.chem.analytical:8345 bionet.biophysics:2816 bionet.molbio.methds-reagnts:56688 newera@plaza.snu.ac.kr wrote: Do not 6M ganidine HCl, 8M urea or some zwitter-ionic detergent make any difference to cation exchange chromatography or affinity chromatography? My protein tends to be aggregated during some purification steps such as dialysis or concentration with a Centriprep. In the midst of misfortune, my protein has no cysteines and seems to be easily renatured; so I plan to add 6M guanidine HCl/8M urea and 0.5~2% or more zwitter-ionic detergents such as CHAPS, sarkosyl to my current buffer system(20mM Na Phosphate, pH 6.8). I am deeply concerned that they severely interfere with my protein's binding ability to BioRex70 cation exchange resin, BlueSepharose affinity chromatography resin, to which my protein seems to quite tightly bind since it begins to elute at around 0.5M NaCl, and C18 reverse phase HPLC. Thank you very much. Key word: 6M Guanidine HCl/8M Urea CHAPS/Sarkosyl Cation Exchange Chromatography Affinity Chromatography Reverse Phase HPLC -- email : newera@plaza.snu.ac.kr address : Lee, Ji Hyun Laboratory of Physical Pharmacy(Prof. Lee, Bong Jin) Seoul National University College of Pharmacy Shinlim-Dong, Kwanak-Gu Seoul 151-742, Korea. Just saw your post, so you may have an answer already, but just in case: Ion exchange chromatography works very well in 8M urea, as long as the urea is first deionized. We pass a 10M urea solution over a standard mixed bed ion-exchange resin before making up buffers for chromatography, and check for complete deionization using a conductivity meter. Although it is possible to store deionized urea solutions at 4 degrees for short periods, I don't recommend it, because urea breaks down, especially at alkaline pH, into reactive species which modify amine groups on proteins, and raise the ionic strength of the solution besides. It's a good idea to make up urea buffers fresh every day if you're going to use them for ion exchange chromatography. Good luck. Trey Simmons Macromolecular Crystallography Facility Lawrence Berkeley Laboratory trey@csg.lbl.gov From owner-biophysics@net.bio.net Mon Apr 14 23:00:00 1997 Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!news.idt.net!usenet.logical.net!news.dal.ca!news.nstn.ca!coranto.ucs.mun.ca!plato.ucs.mun.ca!wmfry From: Mark Fry Newsgroups: bionet.biophysics Subject: reference for 4-AP Date: Tue, 15 Apr 1997 17:19:43 -0230 Organization: Memorial University of Newfoundland Lines: 15 Message-ID: NNTP-Posting-Host: plato.ucs.mun.ca Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: wmfry@plato.ucs.mun.ca Hi. In xenopus muscle cells, there is an inactivating type K+ current (IIK) that is insensitive to the classic blocker (4mM) 4-AP. does anybody know what other agents are effective in blocking Muscle IIK currents? Thanks for any help. Mark Fry ribit @..@ 4th floor BMS Fac of Med. \ (----) Memorial University of NFLD ( >**< ) A1B 3V6 ^^^""""^^^ wmfry@plato.ucs.mun.ca (Xenopus) From owner-biophysics@net.bio.net Tue Apr 15 23:00:00 1997 Path: biosci!daresbury!uninett.no!nntp.uio.no!newsfeeds.sol.net!uwm.edu!news.cse.psu.edu!news.cc.swarthmore.edu!netnews.upenn.edu!taurus.fccc.edu!sauder From: sauder@castor.fccc.edu (John Michael Sauder) Newsgroups: bionet.biophysics Subject: Re: Eternal LIfe Device Date: 15 Apr 1997 21:49:55 GMT Organization: Fox Chase Cancer Center, Philadelphia, PA Lines: 11 Message-ID: <5j0t63$l77$1@taurus.fccc.edu> References: <01bc4447$a08886c0$3bc30b26@itjfvkli> NNTP-Posting-Host: castor.rm.fccc.edu In article <01bc4447$a08886c0$3bc30b26@itjfvkli> "Alex Chiu" writes: >NEW INVENTION ALLOWS HUMANS TO LIVE FOREVER > > [snip] > The speeded up blood circulation and electric current generate faster > metabolism in order to fight aging process. > [snip] Silly me. And I thought an increase in the rate of metabolism would increase the rate of aging. I guess that's why mice live longer than whales. From owner-biophysics@net.bio.net Tue Apr 15 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: "Rohan H. Wickramasinghe" Newsgroups: bionet.biophysics Subject: Dielectric constants/ cytochrome P450 Date: 16 Apr 1997 18:52:27 +0100 Organization: Institute for Tropical Environmental Studies Lines: 35 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5j33kr$r3j@mserv1.dl.ac.uk> Original-To: biophys.@dl.ac.uk Hello, Some time ago we published some papers relating to the possible influence of the dielectric constant on certain cytochrome P450- dependent enzyme activities. We would be very grateful for any information as to whether any of this work has been followed up and for relevant references (esp. in conference proceedings, books etc.). The publications concerned were: 1) R.H.Wickramasinghe, " Regulation of corticosteroid hydroxylations: the intramitochondrial dielectric constant and proteolipids of adrenodoxin and cytochrome P450 ", CYTOBIOS (Cambridge, England) 8(1973)81-94, 2) R.H.Wickramasinghe, " The regulation of corticosteroid hydroxylations. 1. An effect of inorganic ions in regulating iron- sulphur protein-dependent electron transport ", JOURNAL OF BIOENERGETICS 5(1973)151-161, 3) R.H.Wickramasinghe, " Dielectric constants and lithium ion treatment of psychiatric illness " , Abstracts, 35th Annual National Meeting of the American Federation for Clinical Research, 30 April - 1 May 1978 at San Francisco, California, CLINICAL RESEARCH 26(1978) 297A. Thank you very much for taking the time to read this and in advance for any help you can give. Regards, Rohan H.Wickramasinghe (e-mail: rohan@ites.ac.lk) From owner-biophysics@net.bio.net Tue Apr 15 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: "Rohan H. Wickramasinghe" Newsgroups: bionet.biophysics Subject: Cytochrome P450 book Date: 16 Apr 1997 18:52:38 +0100 Organization: Institute for Tropical Environmental Studies Lines: 34 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5j33l6$r4i@mserv1.dl.ac.uk> Original-To: toxicol@dl.ac.uk, ppg@dl.ac.uk, p450@dl.ac.uk, biophys@dl.ac.uk, cellbiol@dl.ac.uk, neur-sci@dl.ac.uk, mol-evol@dl.ac.uk, peptides@dl.ac.uk, neur-sci@dl.ac.lk, mol-evol@dl.ac.uk, cellbiol@dl.ac.uk Hello, * THE CYTOCHROME P-450 PROTEINS: ENVIRONMENTAL AND GENERAL ASPECTS * by ROHAN H. WICKRAMASINGHE , B.A.(Hons)Cantab., M.A.Cantab., PH.D.(Edinburgh), FNASSL The Cytochrome P-450 proteins play most important roles in living organisms. The above book (ISBN 955-552-029-9) provides an introduction to the field and includes an extensive bibliography of references to the publications which had appeared up to the time the book was prepared. The author has performed research on cytochrome P-450 and associated enzymatic reactions at a number of leading universities in Europe and the United States, viz. Edinburgh University, University of Texas at Galveston, Harvard University and the University of Muenster in Germany. (He has also performed research in other areas at other institutions such as at the College de France in Paris and at the University of Maryland at College Park.) Copies of the above book are available for purchase. Further information could be obtained from the following address: Dr. Rohan H.Wickramasinghe, FNASSL, Institute for Tropical Environmental Studies, 41 Flower Road, Colombo 7, Sri Lanka (e-mail: rohan@ites.ac.lk) From owner-biophysics@net.bio.net Wed Apr 16 23:00:00 1997 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!torn!newshost.uwo.ca!usenet From: David O'Neill Newsgroups: bionet.biophysics,bionet.general,bionet.molec-model,uwo.biochem Subject: Tunnel Staining Date: Wed, 16 Apr 1997 20:44:01 -0400 Organization: University of Western Ontario, Ca Lines: 6 Message-ID: <33557250.5996@julian.uwo.ca> NNTP-Posting-Host: ts3-19.slip.uwo.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Authenticated: doneill2@ts3-19.slip.uwo.ca X-Mailer: Mozilla 3.0 (Win95; I) Xref: biosci bionet.biophysics:2824 bionet.general:26459 bionet.molec-model:1489 Can anyone tell me about tunnel staining- ie. techniques etc.? I'm particularly interested in necrosis and apoptosis in endothelial cells. Thanks, Dave. P.S. Peter thanks for your suggestions. From owner-biophysics@net.bio.net Wed Apr 16 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!gondor!newshub.sdsu.edu!news.sgi.com!news-peer.sprintlink.net!news.sprintlink.net!sprint!howland.erols.net!torn!newshost.uwo.ca!usenet From: David O'Neill Newsgroups: bionet.biophysics,bionet.general,bionet.molec-model,uwo.biochem Subject: Angiopoietin II Date: Wed, 16 Apr 1997 20:47:29 -0400 Organization: University of Western Ontario, Ca Lines: 5 Message-ID: <33557321.40E3@julian.uwo.ca> NNTP-Posting-Host: ts3-19.slip.uwo.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Authenticated: doneill2@ts3-19.slip.uwo.ca X-Mailer: Mozilla 3.0 (Win95; I) Xref: biosci bionet.biophysics:2826 bionet.general:26461 bionet.molec-model:1491 Can anyone tell me about angiopoietin- ie. techniques etc.? I'm particularly interested in endothelial cell response in disused skeletal muscle in the rodent hindlimb. Thanks, Dave. From owner-biophysics@net.bio.net Wed Apr 16 23:00:00 1997 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!feed1.news.erols.com!howland.erols.net!torn!newshost.uwo.ca!usenet From: David O'Neill Newsgroups: bionet.biophysics,bionet.general,bionet.molec-model,uwo.biochem Subject: Annexin V Date: Wed, 16 Apr 1997 20:44:49 -0400 Organization: University of Western Ontario, Ca Lines: 4 Message-ID: <33557281.5D68@julian.uwo.ca> NNTP-Posting-Host: ts3-19.slip.uwo.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Authenticated: doneill2@ts3-19.slip.uwo.ca X-Mailer: Mozilla 3.0 (Win95; I) Xref: biosci bionet.biophysics:2825 bionet.general:26460 bionet.molec-model:1490 Can anyone tell me about Annexin V- ie. techniques etc.? I'm particularly interested in necrosis and apoptosis in endothelial cells. Thanks, Dave. From owner-biophysics@net.bio.net Wed Apr 16 23:00:00 1997 Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!cpk-news-feed1.bbnplanet.com!news.bbnplanet.com!nih.gov!usenet From: bernard@elsie.nci.nih.gov (Bernard Murray) Newsgroups: bionet.biophysics,bionet.general,bionet.molbio.methds-reagnts Subject: Re: Regeneron Date: 17 Apr 1997 06:59:32 GMT Organization: National Institutes of Health, Bethesda, MD Lines: 36 Message-ID: <5j4hok$834@light.nih.gov> References: <335573C1.118F@julian.uwo.ca> NNTP-Posting-Host: ermintrude.nci.nih.gov X-Newsreader: WinVN 0.99.7 Xref: biosci bionet.biophysics:2828 bionet.general:26463 bionet.molbio.methds-reagnts:56788 In article <335573C1.118F@julian.uwo.ca>, doneill2@julian.uwo.ca says... > >Good Day! >Have you heard of a company called REGENERON in Tarrytown N.Y.? >A scientist by the name of Yancopoulos is doing work there with >angiopoietin II. >Thanks for all your help. >Dave. No, I've never heard of them but I hope he's enjoying his work (somebody somewhere must enjoy their work). I don't know why you've just answered your own question in your previous post on the subject but... Concerning your other posts on assessment of apoptosis a) Contact Trevigen (especially Philip Vanek) as they have excellent protocols for apoptosis assessment by Tdt or klenow labelling and also work with annexin V. If angiopoietin is also a marker I'm sure they are working on it as well. I have no commercial connection with Trevigen but have found them friendly and informative. b) Try switching from bionet.molec-model to bionet.molbio.methds-reagnts as that newsgroup is probably better suited to this kind of question. I've taken the liberty of doing this. I hope that this helps, Bernard Bernard Murray, Ph.D. bernard@elsie.nci.nih.gov (National Cancer Institute, NIH, Bethesda MD, USA) From owner-biophysics@net.bio.net Wed Apr 16 23:00:00 1997 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.gsl.net!news-dc.gsl.net!news.gsl.net!feed2.belnet.be!news.belnet.be!feed1.belnet.be!news.belnet.be!news.rediris.es!news.upc.es!lilith.uab.es!newsmgr From: Jaumet Newsgroups: bionet.biophysics Subject: sgomez Date: Thu, 17 Apr 1997 19:12:14 +0200 Organization: AULA_PIE Lines: 2 Distribution: inet Message-ID: <335659EE.4015@boira.xtec.es> Reply-To: usuari15@boira.xteces NNTP-Posting-Host: gesami.xtec.es Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 3.01Gold (Win95; I) això es un missatge de prova d'un curset que estic fent i com que tenim una molt dolenta configuracio no podem accedir a una news de prova From owner-biophysics@net.bio.net Wed Apr 16 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: Newsgroups: bionet.biophysics Subject: Data of hydrophobicity indices Date: 17 Apr 1997 15:34:26 +0100 Lines: 11 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5j5cdi$4gf@mserv1.dl.ac.uk> Original-To: bio-info@dl.ac.uk, biophys@dl.ac.uk, comp-bio@dl.ac.uk, peptides@dl.ac.uk, proteins@dl.ac.uk, xtal-log@dl.ac.uk Dear netters: I need to read a recent review paper listing various hydrophobicity indices of 20 amino acids, measured or calculated by various methods. I am grateful to anybody for showing me the reference of such a paper via e-mail. My e-mail address: ctzhang@tju.edu.cn Ren Zhang From owner-biophysics@net.bio.net Wed Apr 16 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.biophysics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Apr 1997 02:00:10 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199704170900.CAA01608@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-biophysics@net.bio.net Wed Apr 16 23:00:00 1997 Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.sprintlink.net!news.sprintlink.net!sprint!howland.erols.net!torn!newshost.uwo.ca!usenet From: David O'Neill Newsgroups: bionet.biophysics,bionet.general,bionet.molec-model Subject: Regeneron Date: Wed, 16 Apr 1997 20:50:09 -0400 Organization: University of Western Ontario, Ca Lines: 6 Message-ID: <335573C1.118F@julian.uwo.ca> NNTP-Posting-Host: ts3-19.slip.uwo.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Authenticated: doneill2@ts3-19.slip.uwo.ca X-Mailer: Mozilla 3.0 (Win95; I) Xref: biosci bionet.biophysics:2827 bionet.general:26462 bionet.molec-model:1492 Good Day! Have you heard of a company called REGENERON in Tarrytown N.Y.? A scientist by the name of Yancopoulos is doing work there with angiopoietin II. Thanks for all your help. Dave. From owner-biophysics@net.bio.net Thu Apr 17 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!rill.news.pipex.net!pipex!oleane!jussieu.fr!univ-lille1.fr!ciril.fr!not-for-mail From: Dumas@hemato.u-nancy.fr (Dumas Dominique) Newsgroups: bionet.biophysics,bionet.general,bionet.molec-model,uwo.biochem Subject: Re: Annexin V Date: 18 Apr 1997 07:51:13 GMT Organization: Hématologie, Faculté de Médecine, Nancy, France Lines: 24 Message-ID: <5j795h$ave$1@arcturus.ciril.fr> References: <33557281.5D68@julian.uwo.ca> Reply-To: dumas@hemato.u-nancy.fr NNTP-Posting-Host: micro7.biomed.u-nancy.fr Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: WinVN 0.92.6+ Xref: biosci bionet.biophysics:2832 bionet.general:26476 bionet.molec-model:1494 In article <33557281.5D68@julian.uwo.ca>, David O'Neill says: > > >Can anyone tell me about Annexin V- ie. techniques etc.? I'm >particularly interested in necrosis and apoptosis in endothelial cells. >Thanks, >Dave. Hi, Annexin V is a member of the calcium and phospholipid binding proteins with vascular anticoagulant activity and is found on the cytosolic face of plasma membranes. During apoptosis cells expose phosphatidylserine (PS) to the outer membrane (Fadok et al, J.immunol 1992;148:2207). The number of binding sites for annexin has been reported as 8.8x10E6/ cell for endothelial cells (Van Herdee,Biochem J 1994; 302:305). The stoichiometry of Annexin V binding to PS ranges betwwen 4 and 8 annexin molecules per one PS molecule (Pignault et al, J Mol Biol 1994;236:199 ; Kuypers et al, Blood 1996;3:1179)) All these information provide from documentation (Apoptosis detection Kit for low cytometry detection, R&D Systems, Technical Service) We can also use Tools for apoptosis (Boeringer Mannheim) Annexin-V-FLUOS for flow cytometry or fluorescence microscopy) I am ot using this technique now but I hope also to detect apoptosis on endothelial cells monolayer by fluorescence microscopy. Contact me for more informations Sincerely yours From owner-biophysics@net.bio.net Thu Apr 17 23:00:00 1997 From: ComDep@lottery.powernet.co.uk (Matthew) Newsgroups: bionet.biophysics Subject: Can DNA change from birth to adulthood? Date: Fri, 18 Apr 1997 19:16:25 GMT Reply-To: ComDep@lottery.powernet.co.uk (Matthew) X-Newsreader: Forte Free Agent 1.0.82 NNTP-Posting-Host: ttym02.powernet.co.uk Message-ID: <3357ba5a.0@news.power.net.uk> Lines: 14 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!Quza.UK.peer!news.cableinet.co.uk!news.power.net.uk!ttym02.powernet.co.uk What evidence is there to support or prove otherwise my thesis that DNA in living organisms can change minutely from birth to adulthood, particularly due to learning and/or adaptation to the environment. This issue is crucial. What are the difficulties in comparing each and every gene between samples taken from a young and mature specimen? Could software database technology assist in this comparative process. Who knows more about it? Please email me, I will thankfully reply. Matthew BioLab@lottery.powernet.co.uk From owner-biophysics@net.bio.net Thu Apr 17 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!news.maxwell.syr.edu!news-was.dfn.de!news-kar1.dfn.de!news-stu1.dfn.de!news.belwue.de!news.uni-mannheim.de!usenet From: Joachim Meissner Newsgroups: bionet.biophysics Subject: Re: Eternal LIfe Device Date: Fri, 18 Apr 1997 16:12:49 +0200 Organization: Lehrstuhl für Informatik V Lines: 57 Message-ID: <33578161.787A2EE6@mp-sun1.informatik.uni-mannheim.de> References: <01bc4447$a08886c0$3bc30b26@itjfvkli> NNTP-Posting-Host: mp-pc21.informatik.uni-mannheim.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01Gold (X11; I; Linux 2.0.28 i586) Alex Chiu wrote: > > NEW INVENTION ALLOWS HUMANS TO LIVE FOREVER > > > If you disagree or disbelieve the content enclosed, please don't get upset. > I am just publicizing my theory and my discovery based on my belief and > observation. If you believe my report, or interested, please see my site > http://home.earthlink.net/~alexycchiu > > Press Release > > ACCORDING TO ALEX CHIU, PEOPLE CAN STAY YOUNG AND LIVE FOREVER BY USING HIS > NEW INVENTION 'THE ETERNAL LIFE RINGS' OR 'THE ETERNAL LIFE FOOT BRACES'. > The Eternal Life Rings are rings to be worn on both small fingers of a user > during sleep. The Eternal Life Foot Braces are to be worn on all fingers > of both feet of a user during sleep. Both devices consist simply magnets > and braces which hold magnets onto the fingers of the user. > > Mr. Alex Chiu claims that the magnetic devices, the finger rings or the > foot braces, both are believed to allow the user to stay young physically > forever or to become younger! The inventor explained that the magnetic > pole on the right small finger or on the right foot is opposite to the > poles of the magnets on the left small finger or of the left foot. With a > different pole on each side of the human body, blood circulation and > electric current of the body are propelled. The speeded up blood > circulation and electric current generate faster metabolism in order to > fight aging process. The mentioned devices invented by Alex Chiu are > believed to be able to alter aging or to keep the users young physically as > long as the users wear them every night during sleep. The devices also > cure many handicapnesses and deseases. > > Mr.Alex Chiu is an inventor who is mainly specialized in effects on human > health by magnetic forces. For interested party, please email > alexycchiu@earthlink.net Check out my web site > http://home.earthlink.net/~alexycchiu > tel 415 585 3825 > > pobox 16547 > San Francisco CA 94116 If this device really speed up the metabolism rate it would in fact accelerat the aging process instead of slowing it or even stop or revert it. A speeded up metabolism does not repair DNA better than a slow (why should it) and broken DNA is most certainly the main effect in aging. That's what I have to say (believe it or not Mr.Alex Chiu) Yours Jom -- .------------------------------------------------------------. |Joachim "Jom" Meissner | |Diplomand im Institut fuer Informatik V an der Uni-Mannheim | |Send Emails to meissner@mp-sun1.informatik.uni-mannheim.de | |Or phone me under +49 621 292 3084 | `------------------------------------------------------------' From owner-biophysics@net.bio.net Fri Apr 18 23:00:00 1997 Path: biosci!agate!howland.erols.net!europa.clark.net!newsfeeds.sol.net!hammer.uoregon.edu!news.icm.edu.pl!uw.edu.pl!news.nask.pl!01-newsfeed.univie.ac.at!03-newsfeed.univie.ac.at!news.univie.ac.at!loop.mdy.univie.ac.at!hs From: hs@mdy.univie.ac.at (Hellfried Schreiber) Newsgroups: bionet.biophysics Subject: 2nd announcement - ICMSB97 Date: 18 Apr 1997 18:15:08 GMT Organization: Vienna University, Austria Lines: 251 Sender: hs@loop.mdy.univie.ac.at (Hellfried Schreiber) Message-ID: <5j8dnc$1b08@www.univie.ac.at> NNTP-Posting-Host: loop.mdy.univie.ac.at ------------------- SECOND ANNOUNCEMENT ------------------- S E C O N D I N T E R N A T I O N A L C O N F E R E N C E O N M O L E C U L A R S T R U C T U R A L B I O L O Y >> I C M S B 97 << SEPTEMBER 10 - 14, 1997 VIENNA, AUSTRIA Organized by the Austrian Chemical Society Working Group Biophysical Chemistry Indroduction to the ICMSB'97 ++++++++++++++++++++++++++++ The first International Conference on Molecular Structural Biology (ICMSB) took place in Vienna in September 1995, and was very well received by all who took part, including over 200 participants from more than 20 countries wordwide. The organisers are pleased to announce the Second ICMSB, which will take place from the 10th to 14th September 1997, and will feature internationally renowned speakers. The conference, which will focus on topics covering a number of the most exciting areas in the field, will be opened by one of the pioneers of molecular structural bioloby, Dr.Max Perutz. The following four days of the conference will include sessions on Structure and Prediction, Macromolecular Interactions, Catalysis and Design, and Folding. Vienna - A Unique Conference Location +++++++++++++++++++++++++++++++++++++ The conference will again be located at the Bundeswirtschaftskammer in Vienna. The city is a particularly attractive location for a conference, with its combination of historical buildings, green parks, and modern architecture. Some of the most famous city sights include Schönbrunn Palace and the Hofburg, former homes of the Habsburgs, St. Stephans Cathedral, and the colourful Hundertwasser House. Also unique to Vienna is the Prater park, with its endless green avenues and its funfair, featuring the "Riesenrad" (ferris wheel). Of course, no trip to Vienna would be complete without a visit to one of the many traditional Viennese cafes, for a piece of the famous Sachertorte. Organising committee ++++++++++++++++++++ A.Kungl, P.Andrew, A.Schilk, A.Schrenk Scientific committee ++++++++++++++++++++ H.Tuppy, O.Steinhauser, A.Kungl, H.Schreiber In cooperation with Austrian Academy of Science Federal Ministry for Economic Affairs Federal Ministry of Labour, Health, and Social Affairs Chamber of Commerce __________________________________________ | | | S C I E N T I F I C P R O G R A M M E | | | | | ------------------------------------------ The four sessions fo the conference cover a wide range of topics and experimental methods, which will be presented in the form of plenary lectures, selected short oral communications, and posters. Plenary Lectures ++++++++++++++++ Wednesday, 10th: Honorary Lecture ================================= M.Perutz (MRC, Cambridge) Glutamine Repeats and Inherited Neurodegenerative Diseases Thursday 11th: Structure and Prediction ======================================= H.Michl (MPI, Fankfurt) Structure and Possible Mechanism of Action of the Cytochrome c Oxidase form Paracoccus denitrificans D.Moras (CNRS, Strasbourg) The Crystal Structure of Nuclear Receptors and Functional Correlations T.Richmond (ETH, Zuerich) X-Ray Structure of the Nucleosome Core Particle at 2.8A Resolution G.Rose (John Hopkins University) Simulation of Protein Folding using LINUS B.Rost (EMBL, Heidelberg) Learing from Evolution to Predict Protein Structure S.Benner (University of Florida) Natural History and the Physical Sciences: Predicting the Structure of Proteins M.Sippl (University of Salzburg) Moluclar Forces in Protein Folding and Prediction Friday 12th: Macromolecular Interactions ======================================== O.Jardetzky (Stanford University) Protein Dynamics, NMR and Protein Function A.Frankel (UCSF, San Francisco) Design and Evolution of RNA-binding Proteins T.Seitz (Yale University) Structural Diversity and Mechnistic Similarity in RNA and DNA Polymerase Families R.Rigler (Karolinska Inst., Stockholm) Fluoresence Correlation Spectroscopy, Detection and Selection of Single Molecules A.Watts (University of Oxford) Atomistic Resolution af Bound Ligands in Functionally Active, Membrane Receptors using Non-Crystallographic Methods Saturday 13th: Catalysis and Design =================================== W. van Gunsteren (ETH, Zuerich) Calculation of Free Energy and Binding Constants for Biomolecular Complexes A.Wlodawer (NCI, Frederick) Retroviral Integrases - the Last Frontier in Designing Drugs against Aids R.Goody (MPI, Dortmund) Problems and Perspectives in Designing Drugs Against AIDS M.Weir (Glaxo Wellcome, Stevenage) Role of Structural Biology in Drug Discovery K.Mueller (F.Hoffmann-La Roche, Basel) Combined Rational and Random Design Concepts in Drug Discovery M.Gruetter (Novartis, Basel) A Target Based Stragegy for Drug Discovery Sunday 14th, Folding ==================== R.Baldwin (Stanford University) Nature of the APomyoglobin Folding Pathway K.Dill (UCSF, San Francisco) Sightseeing along the Landscapes of Protein Folding C.Dobson (University of Oxford) Exploring the Structural Bases of Protein Folding P.Schuster (University of Vienna) RNA Structures beyond the One Sequence-One Structure Paradigm F.Hartl (HHMI, New York) Chaperone-Assisted Protein Folding Call for Posters ++++++++++++++++ Interested participants are invited to submit abstracts describing original work which has not been presented elsewhere. Abstracts should arrive no later than July 10th, 1997. Authors will be informed about the provisional acceptance ot the abstract by the end of July. The presentation of the abstracts as a poster will be confirmed, and included in the Book of Abstracts when one or more of the authors register for the conference. Contributors of outstanding abstracts will be chosen by the Scientific Committee to give a 15 minute oral presentation. Exhibition ++++++++++ An exhibition of instruments, accessories, software, literature and other items is planned. Companies interested in displaying their products are kindly requested to contact the conference secretariat. Registration Fees +++++++++++++++++ before August 1 after August 1 Regular Participant 5000 ATS 5600 ATS GOeCH Member 4000 ATS 4500 ATS Student 2500 ATS 3000 ATS Accomp. Person 500 ATS 600 ATS The registration fee includes the COnference Proceedings and participation in all scientific sessions, the welcome drink, lunch from Thursday to Sunday, coffee breaks, and the participation in the social programme. Accompanying people attend only the social proramme. Location ++++++++ Wirtschaftskammer Oesterreichs (Julius Raab Saal) Wiedner Hauptstrasse 63 A-1040 Vienna If your are interested in a folder of the second announcement including a registration form and the social program, please contact: Dr. Andreas Kungl Gesellschaft Oesterreichischer Chemiker AG Biophysikalische Chemie Nibelungengasse 11 A-1010 Wien, Austria Tel.: ++ 43 1 5874249 or ++ 43 1 5873980 FAX.: ++ 43 1 5878966 e-mail: biophys@goech.co.at Distribution: Followup-To: Organization: Univ. of Vienna, Inst. for Theoretical Chemistry Keywords: -- +-----------------------------------------------------------------------------+ | | | Hellfried Schreiber, Ph.D. | | | +---------------------------------------+-------------------------------------+ | | | | Institute for Theoretical Chemistry | | | Theoretical Biochemistry Group | Mail: hs@mdy.univie.ac.at | | Waehrigerstrasse 17 | Voice: +43 1 40480 - 618 | | A-1090 Wien, Austria, Europe | FAX: +43 1 4028525 | | | | +-----------------------------------------------------------------------------+ From owner-biophysics@net.bio.net Fri Apr 18 23:00:00 1997 Path: biosci!POST.KRASCIENCE.RSSI.RU!bugar From: bugar@POST.KRASCIENCE.RSSI.RU (Nikolai Bugaenko) Newsgroups: bionet.biophysics Subject: Re: Can DNA change from birth to adulthood? Date: 18 Apr 1997 23:39:13 -0700 Organization: Krasnoyarsk Computing Center of RAS Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: <3358438D.4B34@post.krascience.rssi.ru> References: <97418192226.~INN-MSGa00253.bionet-news@dl.ac.uk> Reply-To: bugar@post.krascience.rssi.ru NNTP-Posting-Host: net.bio.net Matthew wrote: > > What evidence is there to support or prove otherwise my thesis that > DNA in living organisms can change minutely from birth to adulthood, > particularly due to learning and/or adaptation to the environment. > > This issue is crucial. What are the difficulties in comparing each and > every gene between samples taken from a young and mature specimen? > > Could software database technology assist in this comparative process. > > Who knows more about it? Please email me, I will thankfully reply. > > Matthew > BioLab@lottery.powernet.co.uk There are a lot of opposite evidences. DNA stays the same during the whole lifetime. No changes could occur in it due to any learning and/or adaptation. By the way, just that one idea - that learned features could be inherited - was the basic issue in Lysenko's theory. Alas, no support of the original idea of yours is available... Michael G.Sadovsky Institute of Biophysics of SD of RAS; 660036 Russia, Siberia, Krasnoyarsk e-mail: bugar@post.krascience.rssi.ru From owner-biophysics@net.bio.net Fri Apr 18 23:00:00 1997 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.maxwell.syr.edu!news.bc.net!torn!kone!news.ccs.queensu.ca!not-for-mail From: 4jch3@qlink.queensu.ca (Hesketh J Christian) Newsgroups: bionet.biophysics Subject: Ion Channel Web Page Date: 18 Apr 1997 15:59:38 GMT Organization: Queen's University, Kingston Lines: 19 Message-ID: <5j85pa$r30@knot.queensu.ca> NNTP-Posting-Host: qlink.queensu.ca X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] A new web page has just been launched which deals with current research in ion channels. It deals primarily with potassium ion channels, but sodium, chloride and calcium ion channels are also discussed. It contains links to current ion channel researchers, a variety of resources for ion channel researchers, a current reference list for current ion channel studies and a succinct link page which covers the best ion channel resources on the internet. The URL is: http://qlink.queensu.ca/~4jch3/ Sincerely, --------------------------------------------------------------------------- J. Christian Hesketh Queen's University - Kingston, Canada Phone (613) 531-8048 e-mail: 4jch3@qlink.queensu.ca Web Page (Research in Ion Channels): http://qlink.queensu.ca/~4jch3 -Current research in ion channels with a biophysical perspective From owner-biophysics@net.bio.net Sat Apr 19 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!news2.digex.net!news5.digex.net!haven.umd.edu!news.umbc.edu!not-for-mail From: cweiss1@umbc.edu (Christopher Weiss) Newsgroups: bionet.biophysics Subject: Potential energy function for proteins needed Date: 20 Apr 1997 17:35:38 -0400 Organization: University of Maryland, Baltimore County Lines: 8 Message-ID: <5je27a$t6q@umbc8.umbc.edu> NNTP-Posting-Host: umbc8.umbc.edu NNTP-Posting-User: cweiss1 I'm looking for a good potential energy function for calculating the energy of protein structures. Where can I find/read about them. What problems have been encountered in designing them? Christopher Weiss From owner-biophysics@net.bio.net Sat Apr 19 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.ums.edu!haven.umd.edu!news.umbc.edu!not-for-mail From: cweiss1@umbc.edu (Christopher Weiss) Newsgroups: bionet.biophysics Subject: A good simple known protein structure Date: 20 Apr 1997 17:25:25 -0400 Organization: University of Maryland, Baltimore County Lines: 7 Message-ID: <5je1k5$sbl@umbc8.umbc.edu> NNTP-Posting-Host: umbc8.umbc.edu NNTP-Posting-User: cweiss1 I'm looking for a simple protein with a well defined structure that has no prosthetic groups and nothing unusual to test an energy minimization program. Does anyone have any suggestions? Christopher Weiss From owner-biophysics@net.bio.net Sat Apr 19 23:00:00 1997 Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!enews.sgi.com!newshub1.home.com!news.home.com!nick.arc.nasa.gov!purdue!haven.umd.edu!news.umbc.edu!not-for-mail From: cweiss1@umbc.edu (Christopher Weiss) Newsgroups: bionet.biophysics Subject: Re: Can DNA change from birth to adulthood? Date: 20 Apr 1997 17:29:25 -0400 Organization: University of Maryland, Baltimore County Lines: 19 Message-ID: <5je1rl$sq0@umbc8.umbc.edu> References: <97418192226.~INN-MSGa00253.bionet-news@dl.ac.uk> <3358438D.4B34@post.krascience.rssi.ru> NNTP-Posting-Host: umbc8.umbc.edu NNTP-Posting-User: cweiss1 >There are a lot of opposite evidences. DNA stays the same during the >whole lifetime. No changes could occur in it due to any learning and/or >adaptation. By the way, just that one idea - that learned features could >be inherited - was the basic issue in Lysenko's theory. > >Alas, no support of the original idea of yours is available... > >Michael G.Sadovsky >Institute of Biophysics of SD of RAS; >660036 Russia, Siberia, Krasnoyarsk >e-mail: bugar@post.krascience.rssi.ru This is false. The DNA of the immune system changes radicaly durring a lifetime. Christopher Weiss From owner-biophysics@net.bio.net Sun Apr 20 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!spring.edu.tw!news.edu.tw!news.ncu.edu.tw!news From: "Jen-Chi ,Chang" Newsgroups: bionet.biophysics Subject: Fractal Properties of Stream Networks Date: 21 Apr 1997 02:55:22 GMT Organization: ¤¤¥¡¤j¾Ç¤g¤ì©Ò¤ô¸ê·½²Õ Lines: 14 Message-ID: <01bc4edd$ac593e40$d13c738c@cjc.NCU.EDU.TW> NNTP-Posting-Host: @140.115.60.209 X-Newsreader: Microsoft Internet News 4.70.1155 Hello I am a graduate student who major in civil engineering in Taiwan, R.O.C. Right now I am investigating about the effect of the fractals characteristics of river networks to the shape of the hydrograph. I come across some problem. I need your help. If you do research or know about the effect of fractals characteristics of river networks to the peak flow, the time to peak, the time base of the hydrograph, could you provide relative information for me ? I appreciate it. Reply my e-mail: s3252070@cc.ncu.edu.tw From owner-biophysics@net.bio.net Sun Apr 20 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!gondor!newshub.sdsu.edu!newshub.csu.net!csulb.edu!hammer.uoregon.edu!news-peer.gsl.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.apfel.de!univ-lyon1.fr!pasteur.fr!jussieu.fr!cea.fr!not-for-mail From: zanotti@amoco.saclay.cea.fr (J.M. Zanotti) Newsgroups: bionet.biophysics Subject: list of the atoms of a protein in contact with the solvent Date: 21 Apr 1997 08:37:00 GMT Organization: Commissariat a l'Energie Atomique (CEA), France Lines: 24 Message-ID: <5jf8vc$9h9$1@news.cea.fr> References: <5je1k5$sbl@umbc8.umbc.edu> Reply-To: zanotti@bali.saclay.cea.fr NNTP-Posting-Host: bali.saclay.cea.fr Keywords: solvent, protein, interface, program Hi, I have the atomic coordinates of the atoms of a protein (PDB file) and I'd like to get the list of the atoms of the protein that are in contact with the solvent. Does anybody have a program or knows a program to do that? I think the method of Lee and Richards (B. Lee and F. Richards,The interpretation of protein structures: estimation of static accessibility, J. Mol. Biol., 55, 379-400 (1971)), could give the good result but I have not his program and perhars there are new methods. Thanks, _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ _/_/_/_/ Jean-Marc Zanotti _/ _/_/_/ _/_/_/_/ Laboratoire Leon Brillouin _/_/_/_/ _/_/_/_/ C.E.A Saclay _/_/_/_/ _/_/_/_/ F-91191 Gif-sur-Yvette Cedex _/_/_/_/ _/_/_/_/ _/_/_/_/ _/_/_/_/ Tel:(33)(0)1-69-08-97-01 _/_/_/_/ _/_/_/_/ Fax:(33)(0)1-69-08-82-61 _/_/_/_/ _/_/_/_/ Email: zanotti@bali.saclay.cea.fr _/_/_/_/ _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ From owner-biophysics@net.bio.net Sun Apr 20 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!newsfeed.nacamar.de!fu-berlin.de!news-ber1.dfn.de!news-fra1.dfn.de!news-ge.switch.ch!news-zh.switch.ch!rzunews.unizh.ch!newsadm From: Rolf Kocherhans Newsgroups: bionet.biophysics Subject: Free practical programs for molecular biologists !!! Date: Mon, 21 Apr 1997 09:39:12 +0100 Organization: University of Zurich Lines: 49 Message-ID: <335B27B0.ED9@vetvir.unizh.ch> Reply-To: rolfk@vetvir.unizh.ch NNTP-Posting-Host: 130.60.22.38 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Macintosh; I; PPC) I wrote a few practical and simple to use programs which facilitate your daily work in the lab such as predicting the size of DNA fragments after digestion (graphical) with restriction enzymes. I would like to make these programs accessible to a broad user group by the Internet. All programs have been tested on MacOS and Windows95. My programs are accessible over the WWW and made functional using Netscape 2.x or Internet Explorer 2.x or higher in association with a free plugin which you have to download and install first. This is what you do: - Download the Roadster plugin http://www.unizh.ch/vetvir/plugin.html) install it on your computer. - Then connect to: http://www.unizh.ch/vetvir/programs.html That's it !! These are my programs which make your live as a molecular biologist easier ! Find here a few more examples or my programs: a. Digest Preview: Enter the size(s) of your DNA fragment(s) and see their migration pattern in a virtual gel in comparison to a 1 kb ladder. b. Adapter Design: Helps to create in frame adapters in order to link incompatible DNA ends together. c. Dilution Calculator: Does all the calculations when you have to make up solutions There are many other programs such as Oligo Tm, Compatible ends etc. Please have a look, comments are welcome! Have fun Rolf Kocherhans mailto:rolfk@vetvir.unizh.ch From owner-biophysics@net.bio.net Sun Apr 20 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!newsfeed.internetmci.com!news.map.com!usenet From: Barbara Foster Newsgroups: bionet.biophysics Subject: Seminar: Optimizing Light Microscopy Date: Sun, 20 Apr 1997 21:33:08 -0700 Organization: Microscopy/Marketing & Education Lines: 50 Message-ID: <335AEE04.544D@mail.map.com> NNTP-Posting-Host: ppp47.map.com Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 2.01 (Win16; U) Six locations: Monday, April 28 - Marriott Marquis, NYC Tuesday, April 29 - Plainview Plaza-Hotel, Plainview, NY Thursday, May 1 - Tufts Medical School/Multi-Media Resource Center, Boston, MA Friday, May 2 - Tufts Medical School/Multi-Media Resource Center, Boston, MA Tuesday, May 6 - Holidome, Holyoke, MA (Greater Springfield area) * Wednesday, May 7 - Sheraton Hartford, Hartford, CT * * catered lunch available for an additional $15.50 Program: 1. A quick tour around the microscope 2. Alignment tips for reducing headaches, fatigue, and errors 3. Care, cleaning, and troubleshooting 4. Useful principles for understanding images 5. Quick, easy, and often free techniques for improving contrast (includes discussions of Phase and Hoffman Modulation Contrast) 6. Advanced techniques (Fluorescence and DIC) 7. Becoming a better consumer: matching your microscope to your applications 8. The Video connection: cameras, computers, and your microscope Fees: $115 if received before 4/18/97; $125 if received after that date. $50 discount on tuition for third person registered from the same facility. Free with your tuition: “Optimizing Light Microscopy for Biological and Clinical Laboratories” Over 220 pages of helpful tips, quick experiments, and new ideas for getting the best from your microscope (ASCLS/Kendall-Hunt, 1997) For further details, contact : Dr. Kenneth Piel or Barbara Foster Microscopy/Microscopy Education (MME) 53 Eton Street Springfield, MA 01108 Phone: (413)746-6931 Fax: (413)746-9311 email: mme@map.com To register: Fax or mail the form below to the MME office Name: _________________________________________________________________ School/Hospital/Company:_________________________________________________Address: ________________________________________________________________ City/State/Zip: ___________________________________________________________ Phone: ____________________Fax: _______________email: ___________________ Check enclosed (payable to MME) for: _________________ [ ] tuition only [ ] tuition plus lunch [ ] VISA [ ] Master Card Name on credit card: __________________________________________________ Card #: ____________________________________ Expiration date: __________ From owner-biophysics@net.bio.net Sun Apr 20 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!newsxfer.itd.umich.edu!news2.acs.oakland.edu!newsfeed.concentric.net!news-master!news From: ucsense Newsgroups: bionet.biophysics Subject: No-Brainer Date: Mon, 21 Apr 1997 17:47:45 -0500 Organization: Save Valuable Time Society Lines: 4 Message-ID: <335BEE91.766D@concentric.net> Reply-To: ucsense@concentric.net NNTP-Posting-Host: cnc097051.concentric.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0C (Win95; I) Save yourself some valuable time. Check out this site: http://www.nobrainergifts.com Bob From owner-biophysics@net.bio.net Mon Apr 21 23:00:00 1997 Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!ais.net!uunet!in3.uu.net!128.255.40.11!news.uiowa.edu!news From: brian-baker@uiowa.edu (Brian M. Baker) Newsgroups: bionet.biophysics Subject: Re: list of the atoms of a protein in contact with the solvent Date: 22 Apr 1997 15:34:36 GMT Organization: Department of Biochemistry, University of Iowa Lines: 23 Message-ID: <5jilqc$q0q@flood.weeg.uiowa.edu> References: <5je1k5$sbl@umbc8.umbc.edu> <5jf8vc$9h9$1@news.cea.fr> NNTP-Posting-Host: reaper.biochem.uiowa.edu X-Newsreader: EmTec News for OS/2 [version: 4.6b] In message <5jf8vc$9h9$1@news.cea.fr> - zanotti@amoco.saclay.cea.fr (J.M. Zanotti) writes: :> :>Hi, :> :>I have the atomic coordinates of the atoms of a protein (PDB file) and I'd :>like to get the list of the atoms of the protein that are in contact with the :>solvent. :> :>Does anybody have a program or knows a program to do that? I think the method :>of Lee and Richards (B. Lee and F. Richards,The interpretation of protein :>structures: estimation of static accessibility, J. Mol. Biol., 55, 379-400 :>(1971)), could give the good result but I have not his program and perhars :>there are new methods. We use such programs on a daily basis. I recommend the program NACCESS, an implemenation of the Lee & Richards algorithm; its free for academic use (requires sending in a simple license form). You'll find a link to it on our web site at: http://boltzmann.biochem.uiowa.edu/~web/asa/asa.html Brian ========================================================== Brian M Baker Email: brian-baker@uiowa.edu Department of Biochemistry Lab phone: (319) 335-7936 From owner-biophysics@net.bio.net Mon Apr 21 23:00:00 1997 Path: biosci!LEX.LCCC.EDU!rcb1 From: rcb1@LEX.LCCC.EDU (Ron Blue) Newsgroups: bionet.biophysics Subject: Re: human aura photo Date: 22 Apr 1997 05:48:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <5jh3aa$ro9$1@nntp.igs.net> NNTP-Posting-Host: net.bio.net My understanding is that auras are due to electric discharge through mosture and the process is well understood and not worth studying, Ron Blue On 22 Apr 1997, george r. harrison wrote: > > e-mail me at gharri@igs.net for human aura photo of four square inches of aura > > processed so you can see images. These types of images cover the human body > space. > > > From owner-biophysics@net.bio.net Mon Apr 21 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!torn!nntp.igs.net!not-for-mail From: gharri@igs.net (george r. harrison) Newsgroups: bionet.biophysics Subject: human aura photo Date: 22 Apr 1997 01:12:42 GMT Organization: IGS - Information Gateway Services Lines: 5 Message-ID: <5jh3aa$ro9$1@nntp.igs.net> NNTP-Posting-Host: ttyc08.ott.igs.net Mime-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII X-Newsreader: WinVN 0.99.8 (16bit) e-mail me at gharri@igs.net for human aura photo of four square inches of aura processed so you can see images. These types of images cover the human body space. From owner-biophysics@net.bio.net Mon Apr 21 23:00:00 1997 Path: biosci!TTUHSC.EDU!phyan From: phyan@TTUHSC.EDU (Alan Neely) Newsgroups: bionet.biophysics Subject: Nobrainer, free money, sex etc. Date: 22 Apr 1997 09:26:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.1.32.19970422113037.006a1180@ttuhsc.edu> NNTP-Posting-Host: net.bio.net Is there a way to prevent automatic posting to reach this newgroup ? A large fraction of the mail I receiver are some kind of junk advertizing. Alan Neely phyan@ttuhsc.edu From owner-biophysics@net.bio.net Mon Apr 21 23:00:00 1997 Path: biosci!UCONNVM.UCONN.EDU!RHODES From: RHODES@UCONNVM.UCONN.EDU Newsgroups: bionet.biophysics Subject: Re: Nobrainer, free money, sex etc. Date: 22 Apr 1997 11:44:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <970422.144501.EDT.RHODES@UConnVM.UConn.Edu> References: NNTP-Posting-Host: net.bio.net Do we hear a volunteer to moderate the newsgroup to filter out the trash? Some of the other groups have adopted this method of eliminating the garbage and keeping the good stuff, and it appears to work well. We could decide appropriate criteria in advance to ensure that anything scientific would pass muster whether or not it appeared controversial or "unusual"... All we need is someone(s) to take the job! | O==O | | DAVID G. RHODES O==O PHONE 860-486-5413 | | SCHOOL OF PHARMACY; U-92 O==O FAX 860-486-4998 | | UNIVERSITY OF CONNECTICUT O==O | | STORRS, CT 06269-2092 O==O RHODES@UCONNVM.UCONN.EDU | | O==O | From owner-biophysics@net.bio.net Mon Apr 21 23:00:00 1997 Path: biosci!BIOSCI.MBP.MISSOURI.EDU!baskin From: baskin@BIOSCI.MBP.MISSOURI.EDU (Tobias Baskin) Newsgroups: bionet.biophysics Subject: Re: Nobrainer, free money, sex etc. Date: 22 Apr 1997 10:48:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 45 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Greetings, The easiest way to do this is to get someone to agree to moderate the list. This means that all messages get prescrened. This has happened to the arabidopsis list that I also join and it has essentially elimanated the spams. The ex-NSF custodians will gladly work with anyone who wants to sign up as moderator. It does mean that the time between posting and replies gets a bit longer. So that's the trade off. On the arabidopsis list, they had a vote of the subscribers first. My two quarks, Tobias >I agree with Alan. It is getting bad enough that I have thought seriously >about unsubscribing to this list. > >Julio. > > > > >>Is there a way to prevent automatic posting to reach this newgroup ? >>A large fraction of the mail I receiver are some kind of junk advertizing. >> >> >>Alan Neely >>phyan@ttuhsc.edu > >Julio C. de Paula >Associate Professor of Chemistry >Haverford College >Haverford, PA 19041 >phone: 610-896-1217 >fax: 610-896-4904 >email: jdepaula@haverford.edu _ ____ ^ __ ____ Tobias I. Baskin / \ / / \ / \ \ University ofMissouri / | / / \ \ \ BiologicalSciences /___/ /__ /___ \ \ \__ 109 Tucker Hall / / / \ \ \ Columbia, MO 65211 USA / / / \ \ \ voice: 573-882-0173 / /____ / \ \__/ \____ fax: 573-882-0123 From owner-biophysics@net.bio.net Mon Apr 21 23:00:00 1997 Path: biosci!HAVERFORD.EDU!jdepaula From: jdepaula@HAVERFORD.EDU (Julio de Paula) Newsgroups: bionet.biophysics Subject: Re: Nobrainer, free money, sex etc. Date: 22 Apr 1997 10:03:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I agree with Alan. It is getting bad enough that I have thought seriously about unsubscribing to this list. Julio. >Is there a way to prevent automatic posting to reach this newgroup ? >A large fraction of the mail I receiver are some kind of junk advertizing. > > >Alan Neely >phyan@ttuhsc.edu Julio C. de Paula Associate Professor of Chemistry Haverford College Haverford, PA 19041 phone: 610-896-1217 fax: 610-896-4904 email: jdepaula@haverford.edu From owner-biophysics@net.bio.net Mon Apr 21 23:00:00 1997 Path: biosci!elara.tripos.com!zauhar From: zauhar@elara.tripos.com ("Randy Zauhar") Newsgroups: bionet.biophysics Subject: Re: Nobrainer, free money, sex etc. Date: 22 Apr 1997 12:03:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <9704221406.ZM408@xhost4.tripos.com> NNTP-Posting-Host: net.bio.net The downside of an unmoderated news group is the annoyance of junk e-mail, and unless someone wants to spend a bit of time to properly maintain the list we will have to put up with it. It is irritating, but it also takes little time to delete the spams. I think the volume of junk would need to increase significantly before I would be forced to unsubscribe (or even worse, volunteer to moderate!) Randy From owner-biophysics@net.bio.net Tue Apr 22 23:00:00 1997 Path: biosci!GUARNERI.CURAGEN.COM!amilosav From: amilosav@GUARNERI.CURAGEN.COM (Aleks Milosavljevic) Newsgroups: bionet.biophysics Subject: Gene Expression and Genetic Networks Date: 23 Apr 1997 08:26:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 141 Sender: daemon@net.bio.net Distribution: world Message-ID: <335E2A7A.446B@curagen.com> NNTP-Posting-Host: net.bio.net /\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\ CALL FOR PAPERS AND ABSTRACTS Gene Expression & Genetic Networks Session 1998 Pacific Symposium on Biocomputing January 5-9, 1998, Hawaii You can also view this call for papers on the WWW: http://www.cgl.ucsf.edu/psb/genexpnet.html We solicit papers and abstracts about computational methods in the monitoring, analysis, and modeling of RNA and protein expression. We are now challenged with understanding how genetically encoded information is transformed into dynamic biomolecular networks defining the organism in health and disease. This session will discuss gene regulatory network models and new methods of acquiring and analyzing large-scale gene expression data. Examples of topics: - Bioinformatics to facilitate collection or analysis of high-throughput RNA or protein expression. - Co-analysis of RNA and protein expression data. - Databases of expression data. - Phenotype - genotype correlation from expression data (e.g. disease, transgenics & knockouts). - Inference of functional relationships from expression time series (development, cell cycle, response to perturbation). - Incorporation of partial knowledge about molecular pathways and cell biology. - Modeling and simulation of expression data. - Information processing in genetic networks. Submission information: PSB'98 will publish accepted full papers in an archival Proceedings. All contributed papers will be rigorously peer-reviewed by at least three referees. Each accepted full paper will be allocated up to 12 pages in the conference Proceedings. The best papers will be selected for a 30-minute oral presentation to the full assembled conference. Accepted poster abstracts will be distributed at the conference separately from the archival Proceedings. To be eligible for Proceedings publication, each full paper must be accompanied by a cover letter stating that it contains original unpublished results not currently under consideration elsewhere. All one page abstracts for posters should be submitted by Oct. 1. Please send electronically to (in plain ascii text or as a Microsoft Word file): altman@smi.stanford.edu. Full papers can be submitted before July 7 by email to: altman@smi.stanford.edu. Format requirements for electronic submission will be available on the web page (http://www.cgl.ucsf.edu/psb) or from Russ Altman (altman@smi.stanford.edu). Alternatively, five copies should be sent by regular mail to: PSB-98 Gene Expression & Genetic Networks Session c/o Section on Medical Informatics Stanford University Medical School, MSOB X215 Stanford CA 94305-5479 USA Gene Expression & Genetic Networks Session Chairs: Barbara Bryant, Millenium Pharmaceuticals (barbara_bryant@msmail.mpi.com) Aleksandar Milosaljevic, CuraGen Corporation (amilosav@curagen.com; http://www.curagen.com) Roland Somogyi, National Institutes of Health (rolands@helix.nih.gov; http://rsb.info.nih.gov/mol-physiol/homepage.html) For more information on PSB98, visit the WWW page at, http://www.cgl.ucsf.edu/psb. /\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\ PSB98 DEADLINES ** July 7 -- Paper submissions due for review. ** Aug 22 -- Final paper decisions announced. ** Sept 15 -- Camera ready copy of papers due. ** Sept 15 -- Early registration deadline. ** Oct 1 -- Poster abstracts due (after this date, posters will be accepted on a space available basis). /\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\ PSB FAQ Q1. How can my paper be included in PSB's hardbound proceedings? PSB publishes peer-reviewed full papers in an archival proceedings. Each accepted paper will be allocated 12 pages in the proceedings volume. Paper authors are required to register (and pay) for the conference by the time they submit their camera-ready copy, or the paper will not be published. Q2. How does a PSB publication compare to a journal publication? PSB papers are strenuously peer reviewed, and must report significant original material. PSB expects to be included in Indicus Medicus, Medline and other indexing services starting this year. All accepted full papers will be indexed just as if they had appeared in a journal. It is too early to assess the impact of a PSB paper quantitatively, but we will take every action we can to improve the visibility and significance of PSB publication. Q3. If I do not want to submit a full paper to PSB, but wish to participate? Authors who do not wish to submit a full paper are welcome to submit one page abstracts, which will be distributed at the meeting separately from the archival proceedings, and are also welcome to display standard or computer-interactive posters. Q4. How can I obtain travel support to come to PSB? We have been able to offer partial travel support to many PSB attendees in the past, including most authors of accepted full papers who request support. However, due to our sponsoring agencies' schedules, we are unable to offer travel awards before the registration (and payment) deadlines for authors. We recognize that this is inconvenient, and we are doing our best to rectify the situation. NO ONE IS GUARANTEED TRAVEL SUPPORT. Travel support applications will be available on our web site (http://www.cgl.ucsf.edu/psb). From owner-biophysics@net.bio.net Wed Apr 23 23:00:00 1997 Path: biosci!daresbury!uninett.no!nntp.uio.no!news.maxwell.syr.edu!news.cis.ohio-state.edu!magnus.acs.ohio-state.edu!mail-news-gateway From: ray@soyokaze.biosci.ohio-state.edu (William C. Ray) Newsgroups: bionet.biophysics Subject: Re: MRI VISUALISATION -- Help/advice needed Date: 24 Apr 1997 12:38:40 -0400 Organization: The Ohio State University Lines: 31 Sender: news@magnus.acs.ohio-state.edu Message-ID: <199704241641.QAA07037@soyokaze.biosci.ohio-state.edu> NNTP-Posting-Host: charm.magnus.acs.ohio-state.edu References: <335BD46C.6201@ae.ic.ac.uk> ohm writes: >I have MRI data comprising of roughly 150 slices through a >persons neck. and >the artery is only about 5x5 >data points large. If this is the _only_ MRI from which you will be needing to extract data, you've probably wasted more time already looking for a solution than it would take you to extract the data by hand. Computers are wonderful toys, and for large-scale data extraction they are imense time savers, but it's quite often the case that for small, non (massively) repetitive problems they are not the best solution. In this case, if a quick, easliy obtainable thresholding program does not readily present itself, and work properly the first time through, it would take you probably a total of 20 minutes to open every one of those images in something like NIH image, select the arterial boundary by hand, and delete the rest of the image. Thresholding then becomes much easier. Will Ray ray@soyokaze.biosci.ohio-state.edu From owner-biophysics@net.bio.net Wed Apr 23 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.maxwell.syr.edu!news-peer.sprintlink.net!news.sprintlink.net!sprint!ais.net!uunet!in3.uu.net!138.95.18.2!wilbur.sequent.com!newsfeed.orst.edu!newshub.tc.umn.edu!newsstand.tc.umn.edu!limerick.cbs.umn.edu!hiroki From: hiroki@limerick.cbs.umn.edu (Hiroki) Newsgroups: bionet.biophysics Subject: Re: list of the atoms of a protein in contact with the solvent Date: 24 Apr 1997 02:25:51 GMT Organization: University of Minnesota Lines: 22 Message-ID: <5jmgbf$2f9@epx.cis.umn.edu> References: <5je1k5$sbl@umbc8.umbc.edu> <5jf8vc$9h9$1@news.cea.fr> <5jilqc$q0q@flood.weeg.uiowa.edu> Reply-To: hiroki@limerick.cbs.umn.edu NNTP-Posting-Host: limerick.cbs.umn.edu X-Newsreader: TIN [version 1.2 PL2] Brian M. Baker (brian-baker@uiowa.edu) wrote: : We use such programs on a daily basis. I recommend the program NACCESS, an : implemenation of the Lee & Richards algorithm; its free for academic use : (requires sending in a simple license form). You'll find a link to it on our : web site at: : http://boltzmann.biochem.uiowa.edu/~web/asa/asa.html : Brian I've tried using probes from 1 to 1000Angstroms in naccess and am getting identical images if I read the *.ASA output file in Rasmol, colored by "temperature" I would have expected to see changes in accessibility in some of the crevices with increasing probes... Am I misinterpreting how the Lee and Richards algorithm works, or am I getting the Bfactor and occupancy fields reversed in my mind? Will play with it some more later, and summarize if anyone sends me suggestions or comments. Hiroki hiroki@limerick.cbs.umn.edu From owner-biophysics@net.bio.net Thu Apr 24 23:00:00 1997 Path: biosci!daresbury!lyra.csx.cam.ac.uk!news.ox.ac.uk!news From: Brian Marsden Newsgroups: bionet.biophysics,bionet.structural-nmr Subject: Iain Campbell group structures - now with added Java! Date: Fri, 25 Apr 1997 11:37:32 +0100 Organization: Biochemistry Dept. University of Oxford Lines: 18 Message-ID: <3360896C.41C6@bioch.ox.ac.uk> NNTP-Posting-Host: nmry.ocms.ox.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (X11; I; IRIX 5.3 IP22) Xref: biosci bionet.biophysics:2872 bionet.structural-nmr:1894 Due to the refurbishment of the Oxford Centre for Molecular Sciences (OCMS) web site, the URL for the structures solved by NMR in Iain Campbell's group has been changed. They can now be found via the IDC section of the OCMS website: http://www.ocms.ox.ac.uk/ or alternatively: http://www.ocms.ox.ac.uk/idc/structures/ A new feature is the addition of the WebMol Java applet to our site that allows most of the structures to be viewed and rotated on screen by a Java-capable browser. Any questions/comments e.t.c. please email Brian Marsden (marsden@bioch.ox.ac.uk). From owner-biophysics@net.bio.net Thu Apr 24 23:00:00 1997 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.nacamar.de!news-kar1.dfn.de!news-fra1.dfn.de!news-ge.switch.ch!serra.unipi.it!news.caspur.it!news1.mclink.it!usenet From: Enzo Di Giulio Newsgroups: bionet.biophysics Subject: On the concept of order in physics Date: Thu, 24 Apr 1997 02:01:13 -0700 Organization: MC-link The World On Line Lines: 90 Message-ID: <335F2159.6495@mclink.it> NNTP-Posting-Host: pgw-179.mclink.it Mime-Version: 1.0 Content-Type: application/octet-stream Content-Transfer-Encoding: base64 X-Mailer: Mozilla 2.0 (Win16; I) Content-Disposition: attachment; filename="ORDERABS.DOC" 26UtAB9AEAQAAAAALQAAAAAAAAAAAAAAgAEAAJQMAAD7EgAAAAAAAAAAAAAAAAAAAAAAABQL AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAASAAAkAAASAAAkACQSAAAAACQS AAAAACQSAAAAACQSAAAAACQSAAAOADISAAAgAFISAAAAAFISAAAAAFISAAAAAFISAAAAAFIS AAAKAFwSAAAKAFISAAAAAGYSAAAoAI4SAAAAAI4SAAAAAI4SAAAAAI4SAAAAAI4SAAAAAI4S 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AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAOAAIAAAAAFAAAAAAAAAQACgEACAAAAAAAACAAAQEABIABAACCAQAA qAEAAKoBAACsAQAADgQAAIgGAACABwAAHAgAAFMJAAB1CgAA1woAANkKAAACCwAARQsAAIsL AACNCwAAuwsAAL0LAADlCwAA6AsAAJQMAAD6+vr69PTu6OLi3Pr6+vr6+vr6+ugAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA BQAAAgAfEAEQbgQABQAABQAfEAEQbgQABQAAAwAfEAEQbgQABQAABAAfEAEQbgQABQAACQAf EAEQbgQABQAAAQAfEAEQbgQVAAADAAARAA4BAEQAAAAYAAAAAAAJBAoABwAAAAAAAAABAADe AAAAABQLAAAQAJQMAAAAAAAAaAoAABQLAAAIAv//AAABACgAAAL//wAAAgAAAIABAACnDAAA BwCAAQAAlAwAAAgAKAASFgBUaW1lcyBOZXcgUm9tYW4ACRYCU3ltYm9sAAgmAEFyaWFsAAAA A4ABABEKAAARCgAACgBNAE0AEQoAAAAAAAARCgAAIgACAAMDjAAAAMQCAACpAQAAAAADqhQm 4qsUJgAAAAAMAG0AAAAAAAAAAAAAAAAAAAAAABkAAAAAAAAAA0VkRwNFZEcAAAAAAAAAAAA= From owner-biophysics@net.bio.net Thu Apr 24 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!news.mathworks.com!fu-berlin.de!news.belwue.de!news.uni-freiburg.de!inn From: "Hilmar Strickfaden" Newsgroups: bionet.biophysics,bionet.general,sci.bio.misc Subject: Who knows the book:"mathematics for the biosciences" ? Date: 25 Apr 1997 21:01:47 GMT Organization: Universität Freiburg Lines: 24 Message-ID: <01bc51bb$e5cbbc80$4b02e684@strickfa> NNTP-Posting-Host: slip75.home.uni-freiburg.de X-Newsreader: Microsoft Internet News 4.70.1157 Xref: biosci bionet.biophysics:2874 bionet.general:26561 sci.bio.misc:8676 Hi there ! I am searching desperately for a book called "MATHEMATICS FOR THE BIOSCIENCES" by Michael R. Cullen. It was published by: PWS-KENT Publishing Company 20 Park Plaza Boston, Massachusetts 02116 The ISBN code is: 0-87150-352-2 I would be very happy for any hint that could lead me to this great book. If anybody knows where I can buy this book and how much it is please write to the following address: strickfa@sun2.ruf.uni-freiburg.de If my request doesn't fit into your news group I'd like to apologize for that. Thank you for your cooperation ! Hilmar Strickfaden From owner-biophysics@net.bio.net Fri Apr 25 23:00:00 1997 Path: biosci!CALPR.COM!steve From: steve@CALPR.COM (Steven Sundberg) Newsgroups: bionet.biophysics Subject: Biophysicist / Chem. Eng. Job Opening Date: 25 Apr 1997 17:15:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Caliper Technologies Corp is an exciting early stage company with the vision of revolutionizing the way laboratory processes are done by developing "Lab on a Chip" technology. Biophysicist / Chemical Engineer Design and run experiments to develop microchip-based formats for biochemical processing. Fun research and feasibility position to explore the technology. Experience with: fluid mechanics, biochemical assays, spectroscopy and fluorescence detection, and novel instrumentation. Electrochemistry is a plus. Ph.D. or equivalent experience. See company website for more information on the company: www.calipertech.com Send e-mail to Anne Kopf-Sill anne@calpr.com or regular mail: Caliper Technologies Corp, 3180 Porter Drive, CA 94304 phone: 415-842-0709 From owner-biophysics@net.bio.net Fri Apr 25 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.mindspring.com!usenet From: krand@pipeline.com (Kemp Randolph) Newsgroups: bionet.biophysics Subject: Re: Can DNA change from birth to adulthood? Date: Sat, 26 Apr 1997 12:55:29 GMT Organization: MindSpring Enterprises, Inc. Lines: 28 Message-ID: <5jsu0k$5bc@camel4.mindspring.com> References: <97418192226.~INN-MSGa00253.bionet-news@dl.ac.uk> <3358438D.4B34@post.krascience.rssi.ru> <5je1rl$sq0@umbc8.umbc.edu> Reply-To: krand@pipeline.com NNTP-Posting-Host: ip149.garden-city.ny.pub-ip.psi.net X-Server-Date: 26 Apr 1997 12:55:48 GMT X-Newsreader: Forte Free Agent 1.0.82 cweiss1@umbc.edu (Christopher Weiss) wrote: >>There are a lot of opposite evidences. DNA stays the same during the >>whole lifetime. No changes could occur in it due to any learning and/or >>adaptation. By the way, just that one idea - that learned features could >>be inherited - was the basic issue in Lysenko's theory. >> >>Alas, no support of the original idea of yours is available... >> >>Michael G.Sadovsky >>Institute of Biophysics of SD of RAS; >>660036 Russia, Siberia, Krasnoyarsk >>e-mail: bugar@post.krascience.rssi.ru >This is false. >The DNA of the immune system changes radicaly durring a lifetime. >Christopher Weiss Any good references on those changes -- an overview , as well as specific instances? Kemp Randolph, Ph.D. NY From owner-biophysics@net.bio.net Fri Apr 25 23:00:00 1997 Path: biosci!TTUHSC.EDU!phyan From: phyan@TTUHSC.EDU (Alan Neely) Newsgroups: bionet.biophysics Subject: Re: Can DNA change from birth to adulthood? Date: 26 Apr 1997 08:46:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.1.32.19970426104423.00779e04@ttuhsc.edu> References: <5jsu0k$5bc@camel4.mindspring.com> NNTP-Posting-Host: net.bio.net At 12:55 PM 4/26/97 GMT, you wrote: >cweiss1@umbc.edu (Christopher Weiss) wrote: > > > >>>There are a lot of opposite evidences. DNA stays the same during the >>>whole lifetime. No changes could occur in it due to any learning and/or >>>adaptation. By the way, just that one idea - that learned features could >>>be inherited - was the basic issue in Lysenko's theory. >>> >>>Alas, no support of the original idea of yours is available... >>> >>>Michael G.Sadovsky >>>Institute of Biophysics of SD of RAS; >>>660036 Russia, Siberia, Krasnoyarsk >>>e-mail: bugar@post.krascience.rssi.ru > >>This is false. > >>The DNA of the immune system changes radicaly durring a lifetime. > >>Christopher Weiss > >Any good references on those changes -- an overview , as well as >specific instances? > > Kemp Randolph, Ph.D. > NY The key is if the DNA changes are inherited and the prevalent view is that gametic cells.. the one that will differentiate on sperm and oocytes do not "adquire" genetic changes during the life span of the individual. This gametic cells are set aside very early during development and several mechanism have evolved to protect the integrity of their DNA. Alan Neely > > From owner-biophysics@net.bio.net Sun Apr 27 23:00:00 1997 Path: biosci!webtv.net!uunet!in2.uu.net!128.255.40.11!news.uiowa.edu!news From: brian-baker@uiowa.edu (Brian M. Baker) Newsgroups: bionet.biophysics Subject: Re: list of the atoms of a protein in contact with the solvent Date: 28 Apr 1997 18:06:55 GMT Organization: Department of Biochemistry, University of Iowa Lines: 41 Distribution: world Message-ID: <5k2ovv$age@flood.weeg.uiowa.edu> References: <5je1k5$sbl@umbc8.umbc.edu> <5jf8vc$9h9$1@news.cea.fr> <5jilqc$q0q@flood.weeg.uiowa.edu> <5jmgbf$2f9@epx.cis.umn.edu> NNTP-Posting-Host: reaper.biochem.uiowa.edu X-Newsreader: EmTec News for OS/2 [version: 4.6b] In message <5jmgbf$2f9@epx.cis.umn.edu> - hiroki@limerick.cbs.umn.edu (Hiroki) writes: :>I've tried using probes from 1 to 1000Angstroms in naccess :>and am getting identical images if I read :>the *.ASA output file in Rasmol, colored by "temperature" :>I would have expected to see changes in accessibility in some of the :>crevices with increasing probes... Am I misinterpreting how the :>Lee and Richards algorithm works, or am I getting the Bfactor :>and occupancy fields reversed in my mind? :>Will play with it some more later, and summarize if anyone sends me :>suggestions or comments. :>Hiroki :>hiroki@limerick.cbs.umn.edu Hmm...you should undoubtedly see different accessibilities, esp. comparing a 1.4 angstrom probe to a 1000 angstrom probe! :) Naccess puts the asa numbers in the place where occupancies normally reside. The last column is the vdw radii of the atom type, I believe, which won't change with probe radius. Its a simple matter to switch the two columns with a C or fortran program (or hack the code, but I don't know if thats allowable under the license agreement...). If that's not a solution, there is another Lee & Richards algorithm out there called access (not to be confused with the original Lee and Richards program, also called access) written by Scott Presnell. It produces nearly identical results as naccess, and in the output the asa of the atoms is in the last column, so its easy to color surfaces by "temperature factor." You can find links to both naccess by Simon Hubbard and access by Scott Presnell on our lab page at http://boltzmann.biochem.uiowa.edu Brian ========================================================== Brian M Baker Email: brian-baker@uiowa.edu Department of Biochemistry Lab phone: (319) 335-7936 University of Iowa From owner-biophysics@net.bio.net Sun Apr