From owner-biophysics@net.bio.net Thu May 01 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!news.maxwell.syr.edu!news-feed.inet.tele.dk!uninett.no!news.uni-c.dk!news.uni-c.dk!ssv2.dina.kvl.dk!not-for-mail From: Jesper Broendum Nielsen Newsgroups: bionet.structural-nmr,bionet.molec-model,sci.med.laboratory,bionet.biophysics,sci.physics Subject: Announcement: NMR Database at http://newton.foodsci.kvl.dk Date: Thu, 01 May 1997 09:45:36 +0200 Organization: Royal Veterinary & Agricultural U., Copenhagen, Denmark Lines: 27 Message-ID: <33684A20.41C6@newton.foodsci.kvl.dk> NNTP-Posting-Host: newton.foodsci.kvl.dk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (X11; I; IRIX 5.2 IP22) CC: jesper Xref: biosci bionet.structural-nmr:1910 bionet.molec-model:1518 sci.med.laboratory:3823 bionet.biophysics:2915 sci.physics:210752 Dear colleges We have put together a database of publications (incl. abstracts) in low resolution NMR. The database is separated in two groups of applications: - Food Applications - Related Applications Visit: http://newton.foodsci.kvl.dk best regards -- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Jesper Broendum, M.Sc. Image Analysis Food Technology and SFK-Technology A/S Vet. and Agri. Uni. Rolighedsvej 30 Vandtaarnsvej 77 1958 Frederiksberg C 2860 Soeborg, DK Ph: (+45) 3528 3205 (+45) 3167 1527 Fax: (+45) 3528 3245 (+45) 3167 9227 E-mail: jesper@newton.foodsci.kvl.dk WWW: newton.foodsci.kvl.dk/jesper ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From owner-biophysics@net.bio.net Thu May 01 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!rill.news.pipex.net!pipex!oleane!jussieu.fr!fdn.fr!r2d2.fdn.org!med.univ-tours.fr!univ-angers.fr!ciril.fr!u-strasbg.fr!news From: simonson@schlitz.u-strasbg.fr (Thomas Simonson) Newsgroups: bionet.molec-model,bionet.xtallography,bionet.software-xplor,bionet.software,bionet.biophysics Subject: Protein Engineering Symposium Date: 2 May 1997 13:34:31 GMT Organization: CRC - Universite Louis Pasteur - Strasbourg France Lines: 54 Message-ID: <5kcqh7$b0@news.u-strasbg.fr> NNTP-Posting-Host: schlitz.u-strasbg.fr Xref: biosci bionet.molec-model:1519 bionet.xtallography:3392 bionet.software:18522 bionet.biophysics:2916 Dear Colleagues, We are pleased to announce a one-day symposium in Strasbourg, May 30th, 1997, on "Protein Engineering: from Experiment to Theory and Back". There are no registration formalities or fee. The program follows. For more information send e-mail to koehl@bali.u-strasbg.fr. We look forward to seeing you. Yours sincerely, Patrice Koehl & Tom Simonson Program: 9:45--10:00 Introduction 10:00--11:00 A. Sali (Rockefeller University) Comparative protein structure modelling for genome projects. 11:00--12:00 H. Hellinga (Duke University) Rational protein design: theory, experiments and applications. Break 14:00--15:00 D. Moras (IGBMC, Strasbourg) Protein--ligand interactions in nuclear receptors of steroid hormones. 15:00--16:00 R. Glockshuber (ETH, Zurich) Random circular permutation of proteins: a new approach to study protein folding. Break 16:30--17:30 M. Karplus (Harvard and Strasbourg Universities) Molecular recognition: free energy simulations and combinatorial ligand design. ----------------------------------------------------------- Thomas Simonson Laboratoire de Biologie Structurale, C.N.R.S., Strasbourg e-mail: simonson@zinfandel.u-strasbg.fr From owner-biophysics@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!bloom-beacon.mit.edu!panix!news-xfer.netaxs.com!netaxs.com!myers From: myers@netaxs.com.NOSPAM (PZ Myers) Newsgroups: bionet.general,bionet.microbiology,alt.life.universe.everything,talk.origins,bionet.biophysics,sci.physics,comp.ai.genetic,bionet.molbio.evolution Subject: Re: questions about embryos Date: Sat, 03 May 1997 09:26:49 -0400 Organization: Net Access - Philadelphia's Original ISP Lines: 41 Message-ID: References: <862648806.30339@dejanews.com> NNTP-Posting-Host: chestnut1-9.slip.netaxs.com Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Yet Another NewsWatcher 2.4.0 Xref: biosci bionet.general:26651 bionet.microbiology:9837 talk.origins:310556 bionet.biophysics:2926 sci.physics:210955 comp.ai.genetic:11091 bionet.molbio.evolution:6027 In article <862648806.30339@dejanews.com>, e904952p@hjc.edu.sg wrote: > I am a high school student seeking some answers.(Somehow I always seem to > start my article by saying that) > Recently I've thinking how can that enormous amount of information all be > stored in one single embryo. Information describing a fully grown > organism! In a sense a complete blueprint is in that embryo. How does this > happen? BIG question...it'll take a little while to explain it. Got a lifetime to spend on it? :-) > I am studying chaos theory and I just learned that very simple dynamic > system can generate extremely complicated behavior. An example is the > famous Madelbrot set.(well it's not exactly a dynamic system but the > message is the same) > Could it be that what is stored in the embryo is a simple mapping, some > instructions telling the embryo how to evolve. All the details such as the > complex network of brain cells simple come about as the embryo blindly > follows the instruction? > Of course external feedback also plays an important role. I'm not sure what you mean by a simple mapping...there's nothing simple about it, and if by "mapping" you mean a one-to-one correspondence between a chunk of code and a structure, no, I don't think so. There is no tidy block of code that is dedicated to instructing neuron X to differentiate a particular transmitter, and to grow an axon that extends 10 microns and then turns left. Rather, cells are programmed with a set of properties and rules that indirectly specify a course of development. A cell may express a receptor that activates a large set of genes if a ligand is bound, another set if it is not bound. Whichever set that is activated modifies the ability of the cell to respond to other signals in the environment, which then modify the ability of the cell to respond to more signals, and so on. The complexity of the nervous system is an emergent property of a progressive and self-referential modulation of gene activity during development. In that sense, it is a lot like the complex dynamic systems modeled on computers. -- Paul Z. Myers http://fishnet.bio.temple.edu/ From owner-biophysics@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!cam-news-feed1.bbnplanet.com!news.bbnplanet.com!cocoa.brown.edu!news From: "Benjamin Mikesh" Newsgroups: bionet.general,bionet.microbiology,alt.life.universe.everything,talk.origins,bionet.biophysics,sci.physics,comp.ai.genetic,bionet.molbio.evolution Subject: Re: questions about embryos Date: 3 May 1997 11:56:15 GMT Organization: Brown University Lines: 20 Message-ID: <01bc57b9$14bf9f40$2fbe9480@feynman.brown.edu> References: <862648806.30339@dejanews.com> NNTP-Posting-Host: gradab-bootp-47.gradcenter.brown.edu X-Newsreader: Microsoft Internet News 4.70.1161 Xref: biosci bionet.general:26650 bionet.microbiology:9835 talk.origins:310542 bionet.biophysics:2925 sci.physics:210946 comp.ai.genetic:11089 bionet.molbio.evolution:6026 > Recently I've thinking how can that enormous amount of information all be > stored in one single embryo. Information describing a fully grown > organism! In a sense a complete blueprint is in that embryo. How does this > happen? > Could it be that what is stored in the embryo is a simple mapping, some > instructions telling the embryo how to evolve? > He Feng > e904952p@hjc.edu.sg Why, yes, that seems reasonable. Doesn't it? Research is going on all the time to study step-by-step embryonic development. Breakthroughs are made constantly in the area of cellular differentiation, which is the hot topic in embryology these days. Since the DNA is the same in every single cell of the body, there really is a finite amount of information which is being utilized by each cell in a different way -- there must certainly also be a line of code in the DNA telling each different kind of cell what DNA sequences to read and what to ignore. -- Ben M. From owner-biophysics@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!daresbury!uninett.no!nntp.uio.no!newsfeeds.sol.net!europa.clark.net!newsfeed2!jump.net!grunt.dejanews.com!not-for-mail Date: Sat, 03 May 1997 04:01:18 -0600 From: hefeng@cz3.nus.sg Subject: questions about embryos Newsgroups: bionet.general,bionet.microbiology,alt.life.universe.everything,talk.origins,bionet.biophysics,sci.physics,comp.ai.genetic,bionet.molbio.evolution Message-ID: <862648806.30339@dejanews.com> Reply-To: e904952p@hjc.edu.sg Organization: Deja News Usenet Posting Service X-Article-Creation-Date: Sat May 03 08:40:11 1997 GMT X-Originating-IP-Addr: 137.132.1.30 (panorama.nus.sg) X-Http-User-Agent: Mozilla/3.01Gold (Win95; I) via Harvest Cache version 3.0pl4-Solaris X-Authenticated-Sender: hefeng@cz3.nus.sg Lines: 24 Xref: biosci bionet.general:26649 bionet.microbiology:9834 talk.origins:310517 bionet.biophysics:2924 sci.physics:210935 comp.ai.genetic:11087 bionet.molbio.evolution:6025 I am a high school student seeking some answers.(Somehow I always seem to start my article by saying that) Recently I've thinking how can that enormous amount of information all be stored in one single embryo. Information describing a fully grown organism! In a sense a complete blueprint is in that embryo. How does this happen? I am studying chaos theory and I just learned that very simple dynamic system can generate extremely complicated behavior. An example is the famous Madelbrot set.(well it's not exactly a dynamic system but the message is the same) Could it be that what is stored in the embryo is a simple mapping, some instructions telling the embryo how to evolve. All the details such as the complex network of brain cells simple come about as the embryo blindly follows the instruction? Of course external feedback also plays an important role. Please send your response to: e904952p@hjc.edu.sg as I don't often have access to Usenet. Thank you. ---------------------------- He Feng e904952p@hjc.edu.sg -------------------==== Posted via Deja News ====----------------------- http://www.dejanews.com/ Search, Read, Post to Usenet From owner-biophysics@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!europa.clark.net!newsfeed2!jump.net!grunt.dejanews.com!not-for-mail Date: Sat, 03 May 1997 03:21:05 -0600 From: hefeng@cz3.nus.sg Subject: LIFE vs DEATH (seeking answers) Newsgroups: bionet.general,bionet.microbiology,alt.life.universe.everything,talk.origins,bionet.biophysics Message-ID: <862647231.29844@dejanews.com> Reply-To: e904952p@hjc.edu.sg Organization: Deja News Usenet Posting Service X-Article-Creation-Date: Sat May 03 08:13:52 1997 GMT X-Originating-IP-Addr: 137.132.1.30 (panorama.nus.sg) X-Http-User-Agent: Mozilla/3.01Gold (Win95; I) via Harvest Cache version 3.0pl4-Solaris X-Authenticated-Sender: hefeng@cz3.nus.sg Lines: 24 Xref: biosci bionet.general:26648 bionet.microbiology:9833 talk.origins:310506 bionet.biophysics:2923 Hi! I am a high school student fascinated by the phenomena of life. I've just discovered the wonder of Usenet so I've been posted a lot recently. One thing I want to ask is that what exactly happens when life leaves a living organism. Physically I don't see anything much has happened. We still got roughly, if not exactly, the same amount of matter there, but still something must have happened, since it's now dead! So what is it? If we consider life as an open system could we say that it's gone though bifurcation and has 'jumped' to another state which is commonly called 'death'? Life is certainly a strange phenomena. If we construct a system and throw in some nutrients and a living organism, some time later we find two organisms in the system. But if we throw in a DEAD organism, nothing will happen no matter how long we wait. Isn't it kind of odd? Please send in you enlightening responses via email at: e904952p@hjc.edu.sg Thank you. ------------------------ He Feng e904952p@hjc.edu.sg -------------------==== Posted via Deja News ====----------------------- http://www.dejanews.com/ Search, Read, Post to Usenet From owner-biophysics@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!newsfeed.internetmci.com!jump.net!grunt.dejanews.com!not-for-mail Date: Sat, 03 May 1997 00:52:04 -0600 From: hefeng@cz3.nus.sg Subject: I am the high school student Newsgroups: bionet.molbio.evolution,talk.origins,sci.physics,bionet.biophysics Message-ID: <862638257.26222@dejanews.com> Reply-To: e904952p@hjc.edu.sg Organization: Deja News Usenet Posting Service X-Article-Creation-Date: Sat May 03 05:44:20 1997 GMT X-Originating-IP-Addr: 137.132.1.30 (panorama.nus.sg) X-Http-User-Agent: Mozilla/3.01Gold (Win95; I) via Harvest Cache version 3.0pl4-Solaris X-Authenticated-Sender: hefeng@cz3.nus.sg Lines: 9 Xref: biosci bionet.molbio.evolution:6024 talk.origins:310475 sci.physics:210916 bionet.biophysics:2922 Hi! I am the high school student who asked about evolution. For the past several days I've received many kind and often enlightning responeses. Here I'd like to thank all of you who bothered to write. -------------- He Feng e904952p@hjc.edu.sg -------------------==== Posted via Deja News ====----------------------- http://www.dejanews.com/ Search, Read, Post to Usenet From owner-biophysics@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!feeder.chicago.cic.net!news.knox.edu!news_admin From: danyell laughlin Newsgroups: bionet.biophysics Subject: evolution Date: Sat, 03 May 1997 10:06:14 -0700 Organization: Another Netscape News Server User Lines: 5 Message-ID: <336B7086.7924@orca.esd114.wednet.edu> NNTP-Posting-Host: 164.116.5.19 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Win95; I) When was the last time you talked to your mothers, your best friends, husbands, siblings? You are wasting a lot of time on something that you can not change. And something that does not have any chance of changing your life. Let it go. Enjoy the things you can do something about. From owner-biophysics@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!daresbury!uninett.no!nntp.uio.no!newsfeeds.sol.net!news.maxwell.syr.edu!newsfeed.direct.ca!nntp.portal.ca!news.bc.net!uvaix3e1.comp.UVic.CA!usenet From: Uncle Al Schwartz Newsgroups: bionet.general,bionet.microbiology,alt.life.universe.everything,talk.origins,bionet.biophysics,sci.physics,comp.ai.genetic,bionet.molbio.evolution Subject: Re: questions about embryos Date: Sat, 03 May 1997 08:04:55 -0700 Organization: The Noble Krell Lines: 41 Message-ID: <336B5417.4610@uvic.ca> References: <862648806.30339@dejanews.com> NNTP-Posting-Host: amca.chem.uvic.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (WinNT; I) To: e904952p@hjc.edu.sg Xref: biosci bionet.general:26653 bionet.microbiology:9839 talk.origins:310598 bionet.biophysics:2929 sci.physics:210977 comp.ai.genetic:11092 bionet.molbio.evolution:6030 hefeng@cz3.nus.sg wrote: > > I am a high school student seeking some answers.(Somehow I always seem to > start my article by saying that) > Recently I've thinking how can that enormous amount of information all be > stored in one single embryo. Information describing a fully grown > organism! In a sense a complete blueprint is in that embryo. How does this > happen? > I am studying chaos theory and I just learned that very simple dynamic > system can generate extremely complicated behavior. An example is the > famous Madelbrot set.(well it's not exactly a dynamic system but the > message is the same) > Could it be that what is stored in the embryo is a simple mapping, some > instructions telling the embryo how to evolve. All the details such as the > complex network of brain cells simple come about as the embryo blindly > follows the instruction? > Of course external feedback also plays an important role. > Please send your response to: e904952p@hjc.edu.sg as I don't often have > access to Usenet. Configuration information is evolved by the dynamics of the system, not by progressively reading off its genetics. Genes specify boundary conditions, interaction rules, and material supply, then things interactively take off. It is a perfectly reasonable hypothesis. As 98+% of the human genome consists of introns - DNA not bearing genetic information - there isn't really all that much space for information storage compard to the demands of the product. One could see how orbits about strange attractors would be resistant to severe reactivity to information contamination, tending to give mostly the same product with mild and multiple perturbations. We are what we are because that is the way things naturally fall together. Good engineering goes with the flow. -- Alan "Uncle Al" Schwartz UncleAl0@ix.netcom.com ("zero" before @) uncleal@uvic.ca (summer only, cAsE-sensitive!) http://www.ultra.net.au/~wisby/uncleal.htm (Toxic URL! Unsafe for children, Democrats, and most mammals) "Quis custodiet ipsos custodes?" The Net! From owner-biophysics@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!ais.net!newsfeed.concentric.net!news-master!cnc19822.concentric.net!user From: president@whitehouse.gov (William A Taylor) Newsgroups: bionet.general,bionet.microbiology,alt.life.universe.everything,talk.origins,bionet.biophysics Subject: Re: LIFE vs DEATH (seeking answers) Date: Sat, 03 May 1997 07:40:04 -0600 Organization: Urmia Institute Lines: 28 Message-ID: References: <862647231.29844@dejanews.com> NNTP-Posting-Host: cnc19822.concentric.net Xref: biosci bionet.general:26652 bionet.microbiology:9838 talk.origins:310557 bionet.biophysics:2927 e904952p@hjc wrote: > Hi! I am a high school student fascinated by the phenomena of life. I've > just discovered the wonder of Usenet so I've been posted a lot recently. > One thing I want to ask is that what exactly happens when life leaves a > living organism. Physically I don't see anything much has happened. We > still got roughly, if not exactly, the same amount of matter there, but > still something must have happened, since it's now dead! > So what is it? > If we consider life as an open system could we say that it's gone though > bifurcation and has 'jumped' to another state which is commonly called > 'death'? > Life is certainly a strange phenomena. If we construct a system and throw > in some nutrients and a living organism, some time later we find two > organisms in the system. But if we throw in a DEAD organism, nothing will > happen no matter how long we wait. > Isn't it kind of odd? I see this as a philosophical question, which will not find many satisfying responses in this newsgroup as this is mostly a scientific forum. Scientific views of life can be called mechanistic which says that living things are merely a more complex arrangement of matter than non-living things. A vitalist view holds that living things are of a different quality than non-living things. I think that your answer lies in the latter which, however, is not the scientific view. -- Bill Taylor, Bill@innocent.com From owner-biophysics@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!feeder.chicago.cic.net!news.knox.edu!news_admin From: Kim Kristensen Newsgroups: bionet.biophysics Subject: internet class Date: Sat, 03 May 1997 17:17:53 -0800 Organization: western washington university Lines: 1 Message-ID: <336BE3C1.56D6@orca.esd114.wednet.edu> NNTP-Posting-Host: 164.116.5.71 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Macintosh; I; PPC) Test message From owner-biophysics@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!europa.clark.net!newsfeed2!feeder.chicago.cic.net!news.knox.edu!news_admin From: joanne doria Newsgroups: bionet.biophysics Subject: class on internet Date: Sat, 03 May 1997 10:11:32 -0700 Organization: Another Netscape News Server User Lines: 1 Message-ID: <336B71C4.1107@orca.esd114.wednet.edu> NNTP-Posting-Host: 164.116.5.18 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Win95; I) This is a test not to keen on the concept of evolution. From owner-biophysics@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!feeder.chicago.cic.net!news.knox.edu!news_admin From: Kim Kristensen Newsgroups: bionet.biophysics Subject: class learning about newsgroup Date: Sat, 03 May 1997 17:13:12 -0800 Organization: western washington university Lines: 2 Message-ID: <336BE2A8.1B83@orca.esd114.wednet.edu> NNTP-Posting-Host: 164.116.5.71 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Macintosh; I; PPC) Good luck with your topic, was your interest in this topic generated from the book "Jurassic Park?" From owner-biophysics@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!feeder.chicago.cic.net!news.knox.edu!news_admin From: Training Account 13 Newsgroups: bionet.biophysics Subject: learner Date: Sat, 03 May 1997 10:03:27 -0700 Organization: Another Netscape News Server User Lines: 3 Message-ID: <336B6FDF.7AC6@orca.esd114.wednet.edu> NNTP-Posting-Host: 164.116.5.66 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Win95; I) Hello, there. I am a 4th grade teacher taking a class on using the Internet. I'm quite ignorant to all this, but I'm trying to learn this at warp speed as our school will be Internet ready by fall. From owner-biophysics@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!feeder.chicago.cic.net!news.knox.edu!news_admin From: Kim Kristensen Newsgroups: bionet.biophysics Subject: class on internet Date: Sat, 03 May 1997 17:14:14 -0800 Organization: western washington university Lines: 1 Message-ID: <336BE2E6.582@orca.esd114.wednet.edu> NNTP-Posting-Host: 164.116.5.71 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Macintosh; I; PPC) This is a test From owner-biophysics@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!feeder.chicago.cic.net!news.knox.edu!news_admin From: "R. colleen Zupancic" Newsgroups: bionet.biophysics Subject: class on news group practice response Date: Sat, 03 May 1997 17:04:05 -0800 Organization: western washington university Lines: 2 Message-ID: <336BE085.56FF@telebyte.com> NNTP-Posting-Host: 164.116.5.71 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Macintosh; I; PPC) Good luck with your project. Was your interest for this topic generate by "Jurassic Park"? From owner-biophysics@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!disgorge.news.demon.net!demon!dispatch.news.demon.net!demon!r832.demon.co.uk!r832.demon.co.uk!Richard From: Richard Steele Newsgroups: bionet.general,bionet.microbiology,alt.life.universe.everything,talk.origins,bionet.biophysics Subject: Re: LIFE vs DEATH (seeking answers) Date: Sun, 4 May 1997 00:49:45 +0100 Organization: mayonnaise Maker Distribution: world Message-ID: References: <862647231.29844@dejanews.com> NNTP-Posting-Host: r832.demon.co.uk X-NNTP-Posting-Host: r832.demon.co.uk MIME-Version: 1.0 X-Newsreader: Turnpike Version 3.02 Lines: 18 Xref: biosci bionet.general:26654 bionet.microbiology:9842 talk.origins:310730 bionet.biophysics:2943 William A Taylor wrote > >e904952p@hjc wrote: > >> Hi! I am a high school student fascinated by the phenomena of life. [etc >> Isn't it kind of odd? > >I see this as a philosophical question, which will not find many >satisfying responses in this newsgroup as this is mostly a scientific >forum. Unlike this one which is a wibble forum. -- Richard - Whose nice comfy chair by the fire has a new squeak ! From owner-biophysics@net.bio.net Sat May 03 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!disgorge.news.demon.net!demon!dispatch.news.demon.net!demon!cawley.demon.co.uk!cawley.demon.co.uk!alec From: Alec Cawley Newsgroups: bionet.general,bionet.microbiology,alt.life.universe.everything,talk.origins,bionet.biophysics Subject: Re: LIFE vs DEATH (seeking answers) Date: Sat, 3 May 1997 23:50:20 +0100 Organization: Home Distribution: world Message-ID: References: <862647231.29844@dejanews.com> NNTP-Posting-Host: cawley.demon.co.uk X-NNTP-Posting-Host: cawley.demon.co.uk MIME-Version: 1.0 X-Newsreader: Turnpike Version 3.03 Lines: 39 Xref: biosci bionet.general:26656 bionet.microbiology:9845 talk.origins:310852 bionet.biophysics:2946 It was said by William A Taylor that > >e904952p@hjc wrote: > >> Hi! I am a high school student fascinated by the phenomena of life. I've >> just discovered the wonder of Usenet so I've been posted a lot recently. >> One thing I want to ask is that what exactly happens when life leaves a >> living organism. Physically I don't see anything much has happened. We >> still got roughly, if not exactly, the same amount of matter there, but >> still something must have happened, since it's now dead! >> So what is it? >> If we consider life as an open system could we say that it's gone though >> bifurcation and has 'jumped' to another state which is commonly called >> 'death'? >> Life is certainly a strange phenomena. If we construct a system and throw >> in some nutrients and a living organism, some time later we find two >> organisms in the system. But if we throw in a DEAD organism, nothing will >> happen no matter how long we wait. >> Isn't it kind of odd? > >I see this as a philosophical question, which will not find many >satisfying responses in this newsgroup as this is mostly a scientific >forum. Scientific views of life can be called mechanistic which says that >living things are merely a more complex arrangement of matter than >non-living things. A vitalist view holds that living things are of a >different quality than non-living things. I think that your answer lies >in the latter which, however, is not the scientific view. "This" may be a scientific forum to you, but because of the uninhibited way the original poster has spammed the newsgroups, he has also hit a completely unscientific wibble group. We shall see what eventuates. Myself, I think it depends how many empties you have lined up when you reply. Sober, I am scientific. A few beers, and I begin to marvel at Life. A few more, and it can't *just* be the chemicals, can it? Just before unconsciousness, vitalism appears to be the prefiect, and obviousm, answer to it all. Alec From owner-biophysics@net.bio.net Sun May 04 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!news.mathworks.com!news-peer.sprintlink.net!news.sprintlink.net!Sprint!newsjunkie.ans.net!newsfeeds.ans.net!lexis-nexis!christw From: christw@lexis-nexis.com (Christopher C. Wood) Newsgroups: bionet.general,bionet.microbiology,talk.origins,bionet.biophysics,bionet.molbio.evolution,comp.ai.genetic,sci.physics,alt.life.universe.everything Subject: Re: conclution on the question of evolution (He Feng) Date: 5 May 1997 17:09:53 GMT Organization: LEXIS-NEXIS, Dayton OH Lines: 45 Message-ID: <5kl491$ea3@mailgate.lexis-nexis.com> References: <862823475.27196@dejanews.com> NNTP-Posting-Host: dolphin.lexis-nexis.com Xref: biosci bionet.general:26685 bionet.microbiology:9859 talk.origins:311238 bionet.biophysics:2970 bionet.molbio.evolution:6062 comp.ai.genetic:11109 sci.physics:211393 In article <862823475.27196@dejanews.com>, hefeng@cz3.nus.sg writes: |> Hi! I am the high school student who raised the question on evolution. [ snip. I liked your summary. ] |> However, one question remains unanswered. |> Why life at all? Why not? |> Is it ture that once the enviroment gets right, life automatically springs |> out? We only have one environment to study. I believe current research shows a small handful of hundreds of millions of years between the end of the planet's bombardment/formation, and the first cells. If a process that takes 200,000,000 years can be called "springs out", then the answer is "so far, yes." |> If so, what's the agency that causes this to happen? What is the agency that causes tornadoes, or hurricanes? The same one applies in this case. One ought not be surprised by organization emerging in an energy flow, in this case of light & heat from the sun warming one side of the earth, and the earth dumping that heat back into space on its side opposite the sun. |> I'd like to thank all those who have kindly shared their knowledge and |> thoughts with me. They have helped me to gain a valuable insight in the |> issue. |> Any commends please post to the newsgroup: talk.origins |> or send your email to: e904952p@hjc.edu.sg |> Thank you. |> ------------------- |> He Feng |> e904952p@hjc.edu.sg |> |> -------------------==== Posted via Deja News ====----------------------- |> http://www.dejanews.com/ Search, Read, Post to Usenet Chris -- Speaking only for myself, of course. Chris Wood christw@lexis-nexis.com cats@CFAnet.com From owner-biophysics@net.bio.net Sun May 04 23:00:00 1997 Path: biosci!POLY.BIOCHEM.WISC.EDU!guttman From: guttman@POLY.BIOCHEM.WISC.EDU (HARRY J. GUTTMAN 608-262-3019) Newsgroups: bionet.biophysics Subject: equilibrium talk Date: 5 May 1997 08:28:16 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 54 Sender: daemon@net.bio.net Distribution: world Message-ID: <97050510103554@poly.biochem.wisc.edu> NNTP-Posting-Host: net.bio.net Hello all, I'd like to give my 2 cents worth on the equilibrium discussion...I try not to think about evolution it makes my head hurt (from expansion?) My bias on this is that I am using thermo and stat mech to describe cellular processes in E. coli. Generally, I think one can argue that something does not have to be in complete equilibrium to study its thermo properties. For example, diamond is not the most stable form of carbon (graphite is). Yet, it is perfectly reasonable to measure various thermo properties of diamond since the TIME SCALE of conversion to graphite is much slower than the measurement. To bolster my point of view, I give the following quote. The best description of equilibrium I know of is in Callen "Thermodynamics and an intro to thermo. stat.", 2nd edition, 1985. I think, Callen is one of the most definitive texts on thermo. On page 15 he writes: "In actuality, few systems are in absolute and true equilibrium. In absolute equilibrium all radioactive materials would have decayed completely .....Such processes, which take cosmic times to complete, generally can be ignored. A system that has completed the relevant processes of spontaneous evolution, and that can be described by a reasonably small number of parameters, can be considered to be in >>metastable equilibrium<<. Such a limited equilibrium is sufficient for the application of thermodynamics. "In practice the criterion for equilibrium is circular. >>Operationally, a system is in an equilibrium state if its properties are consistently described by thermodynamic theory< Newsgroups: bionet.general,bionet.microbiology,talk.origins,bionet.biophysics,bionet.molbio.evolution,comp.ai.genetic,sci.physics,alt.life.universe.everything Subject: Re: conclution on the question of evolution (He Feng) Date: Mon, 5 May 1997 12:54:02 +0100 Organization: mayonnaise Maker Distribution: world Message-ID: <5NMoH4AapcbzEwLR@r832.demon.co.uk> References: <862823475.27196@dejanews.com> NNTP-Posting-Host: r832.demon.co.uk X-NNTP-Posting-Host: r832.demon.co.uk MIME-Version: 1.0 X-Newsreader: Turnpike Version 3.02 Lines: 21 Xref: biosci bionet.general:26674 bionet.microbiology:9854 talk.origins:311185 bionet.biophysics:2962 bionet.molbio.evolution:6052 comp.ai.genetic:11103 sci.physics:211344 wrote > >Hi! I am the high school student who raised the question on evolution. >Since then I've received quite a number of responses. I'd like to present >some of the conclutions of the discussion. >There are mainly two points. >First, many who wrote to me pointed out that evolution does NOT >necessarilly move towards greater complexity. I'll bet even *more* told you to bog off. Can't you take a hint? >I'd like to thank all those who have kindly shared their knowledge and >thoughts with me. They have helped me to gain a valuable insight in the >issue. Another tosser on line -- Richard - Whose nice comfy chair by the fire has a new squeak ! From owner-biophysics@net.bio.net Sun May 04 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!news-peer.gsl.net!newsfeed.internetmci.com!jump.net!grunt.dejanews.com!not-for-mail Date: Mon, 05 May 1997 05:51:17 -0600 From: hefeng@cz3.nus.sg Subject: evolution (He Feng e904952p@hjc.edu.sg) Newsgroups: bionet.general,bionet.microbiology,bionet.biophysics,sci.physics Message-ID: <862828016.28953@dejanews.com> Reply-To: e904952@hjc.edu.sg Organization: Deja News Usenet Posting Service X-Article-Creation-Date: Mon May 05 10:27:00 1997 GMT X-Originating-IP-Addr: 137.132.1.30 (panorama.nus.sg) X-Http-User-Agent: Mozilla/3.01Gold (Win95; I) via Harvest Cache version 3.0pl4-Solaris X-Authenticated-Sender: hefeng@cz3.nus.sg Lines: 20 Xref: biosci bionet.general:26673 bionet.microbiology:9853 bionet.biophysics:2961 sci.physics:211340 Hi! I realised that I've been missing a lot of discussions. (I couldn't access Usenet very often, the government won't allow it) In my article "evolution (please read)" I wrote something about equilibrim. Certainly living organisms are not in equilibrim state. They are not even in near-equilibrim state. In such states there will be no life at all. But life can't occur in systems that are too far from equilibrim either. In such circumstance CHAOS takes place and life can't exist. Living organisms are open sytems (we exchange energy and matter with the outside world), I understand that it's this constant "kick" from outside world that keeps us from equillibrim state (which means death!) but not too far. I am thinking if life itself is a necessary phenomanon, meaning that natural laws leads inevitably to life, maybe it could be somehow explained by bifurcation of open systems. When the "kick" from outside reach a certain critical value, life occurs! ------------- He Feng -------------------==== Posted via Deja News ====----------------------- http://www.dejanews.com/ Search, Read, Post to Usenet From owner-biophysics@net.bio.net Sun May 04 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!news-peer.gsl.net!newsfeed.internetmci.com!jump.net!grunt.dejanews.com!not-for-mail Date: Mon, 05 May 1997 04:33:21 -0600 From: hefeng@cz3.nus.sg Subject: conclution on the question of evolution (He Feng) Newsgroups: bionet.general,bionet.microbiology,talk.origins,bionet.biophysics,bionet.molbio.evolution,comp.ai.genetic,sci.physics,alt.life.universe.everything Message-ID: <862823475.27196@dejanews.com> Reply-To: e904952p@hjc.edu.sg Organization: Deja News Usenet Posting Service X-Article-Creation-Date: Mon May 05 09:11:15 1997 GMT X-Originating-IP-Addr: 137.132.1.30 (panorama.nus.sg) X-Http-User-Agent: Mozilla/3.01Gold (Win95; I) via Harvest Cache version 3.0pl4-Solaris X-Authenticated-Sender: hefeng@cz3.nus.sg Lines: 39 Xref: biosci bionet.general:26670 bionet.microbiology:9852 talk.origins:311179 bionet.biophysics:2960 bionet.molbio.evolution:6050 comp.ai.genetic:11102 sci.physics:211329 Hi! I am the high school student who raised the question on evolution. Since then I've received quite a number of responses. I'd like to present some of the conclutions of the discussion. There are mainly two points. First, many who wrote to me pointed out that evolution does NOT necessarilly move towards greater complexity. Indeed in many circumstances, the reverse happens. At the beginning of life evolution got no where else to go but towards higher complexity and organisation. You can't get simpler than the simplest! However this is not the case as time goes on. Second, the only driving force in the process of evolution is survival. The fittest survives always applies. Most of the time complexity helps oganisms to sruvive better, but sometimes simplicity helps to. That's why we see a large variety of living things now. Bacterias are still around and they are doing pretty well. As shown by the recently reported lizard experiment, the process of evolution is faster than many of us think. It's possible, actually likely, that organs such as the eye could have formed during the years. (Well, human civilization has existed for thousands of years, have we changed much by the drastic change of our living enviroment?) However, one question remains unanswered. Why life at all? Is it ture that once the enviroment gets right, life automatically springs out? If so, what's the agency that causes this to happen? I'd like to thank all those who have kindly shared their knowledge and thoughts with me. They have helped me to gain a valuable insight in the issue. Any commends please post to the newsgroup: talk.origins or send your email to: e904952p@hjc.edu.sg Thank you. ------------------- He Feng e904952p@hjc.edu.sg -------------------==== Posted via Deja News ====----------------------- http://www.dejanews.com/ Search, Read, Post to Usenet From owner-biophysics@net.bio.net Sun May 04 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!news.mathworks.com!rill.news.pipex.net!pipex!oleane!pasteur.fr!jussieu.fr!news.univ-lille1.fr!u-strasbg.fr!news From: pingouin@crystal.u-strasbg.fr (Francois JEANMOUGIN) Newsgroups: bionet.general,bionet.microbiology,alt.life.universe.everything,talk.origins,bionet.biophysics,sci.physics,comp.ai.genetic,bionet.molbio.evolution Subject: Re: questions about embryos Followup-To: talk.origins Date: 5 May 1997 08:37:52 GMT Organization: IGBMC Lines: 26 Message-ID: <5kk690$5gf@news.u-strasbg.fr> References: <862648806.30339@dejanews.com> Reply-To: jeanmougin@igbmc.u-strasbg.fr NNTP-Posting-Host: crystal.u-strasbg.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii X-Newsreader: knews 0.9.8 Xref: biosci bionet.general:26669 bionet.microbiology:9851 talk.origins:311171 bionet.biophysics:2959 sci.physics:211317 comp.ai.genetic:11101 bionet.molbio.evolution:6049 In article <862648806.30339@dejanews.com>, hefeng@cz3.nus.sg writes: [...] > Could it be that what is stored in the embryo is a simple mapping, some > instructions telling the embryo how to evolve.[...] A simple map.... No, it's a colection of data that is express under circonstances. THe difficulty is not to decode that data (for us), it is to understand the mechanisms of interaction between the proteins and higher structures and the data (the genome). The level of complexity in interactions, give the level of complexity in evolution (or about). Francois. P.S.: The neural network is NOT coded in the genome. It results of interactions and stimuli that are interpreted regarding the genome. P.P.S.: All what I wrote is a big reduction of the reality. P.P.S.: So much newsgroup? well so FollowUp-To: talk.origins (sorry if I don't read this group...) -- Francois Jeanmougin Service de bioinformatique / bioinformatics service IGBMC BP 163 67404 Illkirch France tel :(France) 03 88 65 32 71 / (international) (+33) 3 88 65 32 71 e-mail : jeanmougin@igbmc.u-strasbg.fr "C'est pas parcequ'on monte au banc, qu'il faut descendre a jeun." (Thiefaine) From owner-biophysics@net.bio.net Sun May 04 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!news.he.net!newsfeed.nacamar.de!disgorge.news.demon.net!demon!dispatch.news.demon.net!demon!mail2news.demon.co.uk!not-for-mail From: Terrance Richard Boyes Newsgroups: bionet.general,bionet.microbiology,alt.life.universe.everything,talk.origins,bionet.biophysics Subject: Re: LIFE vs DEATH (seeking answers) Followup-To: alt.life.universe.everything Date: Mon, 5 May 97 01:33:24 +0100 (BST) Organization: ECL.net Distribution: world Message-ID: <9705050233.2vf5@pierrot.co.uk> References: <862647231.29844@dejanews.com> X-Mail2News-User: tez@pierrot.co.uk X-Mail2News-Path: punt-2.mail.demon.net!pierrot.demon.co.uk!pierrot.co.uk X-Pierrot-Homepage: X-Organization: Team AMIGA X-Newsreader: TIN [AMIGA 1.3 950726BETA PL0] X-NaMLServ: $VER: v0.9b Lines: 29 Xref: biosci bionet.general:26665 bionet.microbiology:9849 talk.origins:311125 bionet.biophysics:2958 Alec Cawley (alec@cawley.demon.co.uk) wrote: > It was said by William A Taylor that > >e904952p@hjc wrote: > > > >> Hi! I am a high school student fascinated by the phenomena of life. I've > > > >I see this as a philosophical question, which will not find many Not a homework question? > >satisfying responses in this newsgroup as this is mostly a scientific > >forum. Scientific views of life can be called mechanistic which says that > Or not... > > "This" may be a scientific forum to you, but because of the uninhibited > way the original poster has spammed the newsgroups, he has also hit a > completely unscientific wibble group. We shall see what eventuates. You're talking about SeKS again aren't you :) Followup-To: alt.life.universe.everything -- Team AMIGA Meader's Law: Whatever happens to you, it will previously have happened to everyone you know, only more so. From owner-biophysics@net.bio.net Sun May 04 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!news-out.communique.net!communique!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!news-peer.sprintlink.net!news.sprintlink.net!Sprint!howland.erols.net!newshub2.home.com!newshub1.home.com!news.home.com!nick.arc.nasa.gov!purdue!news.bu.edu!usenet From: Chris Colby Newsgroups: bionet.general,bionet.microbiology,talk.origins,bionet.biophysics,bionet.molbio.evolution,comp.ai.genetic,sci.physics Subject: misconception about evolution (response to He Feng) Followup-To: talk.origins Date: Mon, 05 May 1997 11:34:46 +0000 Organization: Boston University Biology Lines: 30 Message-ID: <336DC5D6.570E@biology.bu.edu> References: <862823475.27196@dejanews.com> Reply-To: colby@biology.bu.edu NNTP-Posting-Host: bio-intro4.bu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Macintosh; I; 68K) CC: hefeng@cz3.nus.sg Xref: biosci bionet.general:26684 bionet.microbiology:9858 talk.origins:311237 bionet.biophysics:2969 bionet.molbio.evolution:6061 comp.ai.genetic:11108 sci.physics:211390 I've trimmed the newsgroups line (slightly) and set follow-ups to talk.origins. hefeng@cz3.nus.sg wrote: > Hi! I am the high school student who raised the question on evolution. > Second, the only driving force in the process of evolution is survival. This is wrong. Replace "survival" with "reproduction" and you are back on track. At the very least you are accurately describing natural selection. Fitness is the reproductive rate of a type of organism relative to other types in the population. There are many components to fitness. Survival is one. Fertility, fecundity and sexual attractiveness are some other important components. The alley cat that runs across the road, finds mates and leaves offspring is more fit than one who doesn't run across the raod and find mates. This is true even if the "road runner" gets run over at an earlier age. In addition, there are other mechanisms of evolution besides natural selection; they are: mutation, genetic drift, recombination and gene flow. If you'd like to learn more about evolution, check out my "Introduction to Evolutionary Biology" on the talk.origins web page. (I wrote it when I was a grad. student studying evolution.) It's at: http://www.talkorigins.org/faqs/faq-intro-to-biology.html Chris Colby From owner-biophysics@net.bio.net Sun May 04 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!newshub.csu.net!usc!howland.erols.net!newshub2.home.com!newshub1.home.com!news.home.com!nick.arc.nasa.gov!purdue!news.bu.edu!usenet From: Chris Colby Newsgroups: bionet.general,bionet.microbiology,talk.origins,bionet.biophysics,bionet.molbio.evolution,comp.ai.genetic,sci.physics,alt.life.universe.everything Subject: Re: conclution on the question of evolution (He Feng) Date: Mon, 05 May 1997 11:40:08 +0000 Organization: Boston University Biology Lines: 21 Message-ID: <336DC718.28D1@biology.bu.edu> References: <862823475.27196@dejanews.com> <5NMoH4AapcbzEwLR@r832.demon.co.uk> Reply-To: colby@biology.bu.edu NNTP-Posting-Host: bio-intro4.bu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Macintosh; I; 68K) Xref: biosci bionet.general:26683 bionet.microbiology:9857 talk.origins:311236 bionet.biophysics:2968 bionet.molbio.evolution:6060 comp.ai.genetic:11107 sci.physics:211389 Richard Steele wrote: > >Hi! I am the high school student who raised the question on evolution. > >Since then I've received quite a number of responses. > I'll bet even *more* told you to bog off. Can't you take a hint? It's a high school kid curious about evolution. Do you have to be such an asshole? Is it bad to foster kid's interest in biology and science? > >I'd like to thank all those who have kindly shared their knowledge and > >thoughts with me. > Another tosser on line He's thanking people for responding, and you call him a tosser? Oh, the irony. Get bent Richard. > Richard - Whose nice comfy chair by the fire has a new squeak ! Chris Colby From owner-biophysics@net.bio.net Sun May 04 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!news.mathworks.com!news.maxwell.syr.edu!news.bc.net!news.sfu.ca!fraser!tchowa From: stufnten Newsgroups: bionet.general,bionet.microbiology,talk.origins,bionet.biophysics,bionet.molbio.evolution,comp.ai.genetic,sci.physics,alt.life.universe.everything Subject: Re: conclution on the question of evolution (He Feng) Date: Mon, 5 May 1997 15:54:41 -0700 Organization: Simon Fraser University Lines: 17 Message-ID: References: <862823475.27196@dejanews.com> <5NMoH4AapcbzEwLR@r832.demon.co.uk> <336DC718.28D1@biology.bu.edu> NNTP-Posting-Host: fraser.sfu.ca Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: tchowa@fraser In-Reply-To: <336DC718.28D1@biology.bu.edu> Xref: biosci bionet.general:26692 bionet.microbiology:9861 talk.origins:311315 bionet.biophysics:2978 bionet.molbio.evolution:6070 comp.ai.genetic:11113 sci.physics:211437 On Mon, 5 May 1997, Chris Colby wrote: > It's a high school kid curious about evolution. Do you have to be such > an asshole? Is it bad to foster kid's interest in biology and science? No, but could we all stop the crossposting? This thread has sod-all to do with either life, the universe or.... [...] As I've said, this ng needs a new name. stufnten, Toby Kaufman will suffer Ich weiss keinen besseren Lebenszweck als am Grossen und Unmoeglichen zu Grunde zu gehen - FNietzsche From owner-biophysics@net.bio.net Sun May 04 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!news-peer.gsl.net!howland.erols.net!newshub2.home.com!newshub1.home.com!news.home.com!nick.arc.nasa.gov!purdue!news.bu.edu!usenet From: Chris Colby Newsgroups: bionet.general,bionet.microbiology,talk.origins,bionet.biophysics,sci.physics,comp.ai.genetic,bionet.molbio.evolution Subject: Re: questions about embryos Followup-To: talk.origins Date: Mon, 05 May 1997 17:24:38 +0000 Organization: Boston University Biology Lines: 21 Message-ID: <336E17D7.1D5A@biology.bu.edu> References: <862648806.30339@dejanews.com> <336B5417.4610@uvic.ca> Reply-To: colby@biology.bu.edu NNTP-Posting-Host: bio-intro4.bu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Macintosh; I; 68K) Xref: biosci bionet.general:26691 bionet.microbiology:9860 talk.origins:311314 bionet.biophysics:2977 sci.physics:211435 comp.ai.genetic:11112 bionet.molbio.evolution:6069 Uncle Al Schwartz wrote: > As 98+% of the human genome consists of > introns The human genome is not ninety-eight percent introns. Roughly three percent of our genome is coding DNA. The rest is various classes of repetitive DNA. (Britten and Kohne called them foldback DNA, highly repetitive DNA and middle-repetitive DNA.) The repetitive DNA may be localized or dispersed. Introns are non-coding regions of genes. I don't know what percent of the genome they take up, but since they are part of genes, I'll guess less than three percent. [I grabbed this info from Li and Graur's 1991 book (Molecular Evolution).] > Alan "Uncle Al" Schwartz Chris Colby From owner-biophysics@net.bio.net Sun May 04 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!news.mathworks.com!cam-news-hub1.bbnplanet.com!cpk-news-feed1.bbnplanet.com!news.bbnplanet.com!gwu.edu!not-for-mail From: kneep@gwis2.circ.gwu.edu (Antonio Greaney Delgado) Newsgroups: bionet.general,bionet.microbiology,talk.origins,bionet.biophysics,bionet.molbio.evolution,comp.ai.genetic,sci.physics Subject: Re: conclution on the question of evolution (He Feng) Date: 5 May 1997 19:08:07 -0400 Organization: The George Washington University Lines: 33 Message-ID: <5klp8n$e4l@gwis2.circ.gwu.edu> References: <862823475.27196@dejanews.com> <5NMoH4AapcbzEwLR@r832.demon.co.uk> <336DC718.28D1@biology.bu.edu> NNTP-Posting-Host: gwis2.circ.gwu.edu Xref: biosci bionet.general:26693 bionet.microbiology:9862 talk.origins:311317 bionet.biophysics:2979 bionet.molbio.evolution:6071 comp.ai.genetic:11114 sci.physics:211440 Chris Colby wrote: >Richard Steele wrote: > >> >Hi! I am the high school student who raised the question on evolution. >> >Since then I've received quite a number of responses. > >> I'll bet even *more* told you to bog off. Can't you take a hint? > >It's a high school kid curious about evolution. Do you have to be such >an asshole? Is it bad to foster kid's interest in biology and science? I believe what Richard, in his own inimitable way, is try to say (if I may be so bold, Richard) is that haphazard crossposting is an annoyance to people on the Internet who wish to avoid it. This student posted to several newsgroups that undoubtedly have discussions on this topic. Ours, (alt.life.universe.everything) doesn't (unless something wierd happens (which admittedly is more often than not (but that doesn't always mean that everytime something weird happens we talk about evolution))). Anyway. For those of you still posting in response to any of these messages, please strike "alt.life.universe.everything" from the newsgroup list. So that we may continue to talk about absolutely nothing of consequence. Kneep! P.S. Entropy requires no maintenance. [Note: Alt.life.universe.everything has already been struck from the ng list in this post.] -- From owner-biophysics@net.bio.net Mon May 05 23:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news-peer.sprintlink.net!news.sprintlink.net!Sprint!howland.erols.net!panix!cmcl2.nyu.edu!news.nyu.edu!scholar.nyu.edu!not-for-mail From: gans@scholar.nyu.edu (Paul J. Gans) Newsgroups: bionet.general,bionet.microbiology,talk.origins,bionet.biophysics,bionet.molbio.evolution,comp.ai.genetic,sci.physics,alt.life.universe.everything Subject: Re: conclution on the question of evolution (He Feng) Followup-To: bionet.general,bionet.microbiology,talk.origins,bionet.biophysics,bionet.molbio.evolution,comp.ai.genetic,sci.physics,alt.life.universe.everything Date: 6 May 1997 03:41:02 GMT Organization: New York University Lines: 25 Distribution: world Message-ID: <5km98e$fc4$2@news.nyu.edu> References: <862823475.27196@dejanews.com> <5NMoH4AapcbzEwLR@r832.demon.co.uk> NNTP-Posting-Host: scholar.chem.nyu.edu X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Xref: biosci bionet.general:26700 bionet.microbiology:9864 talk.origins:311379 bionet.biophysics:2980 bionet.molbio.evolution:6072 comp.ai.genetic:11118 sci.physics:211515 Richard Steele (Richard@r832.demon.co.uk) wrote: : : wrote : : : > : >Hi! I am the high school student who raised the question on evolution. : >Since then I've received quite a number of responses. I'd like to present : >some of the conclutions of the discussion. : >There are mainly two points. : >First, many who wrote to me pointed out that evolution does NOT : >necessarilly move towards greater complexity. : : I'll bet even *more* told you to bog off. Can't you take a hint? : : >I'd like to thank all those who have kindly shared their knowledge and : >thoughts with me. They have helped me to gain a valuable insight in the : >issue. : : Another tosser on line Uncalled for. ------ Paul J. Gans [gans@scholar.chem.nyu.edu] From owner-biophysics@net.bio.net Mon May 05 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!news.mathworks.com!news.maxwell.syr.edu!disgorge.news.demon.net!demon!dispatch.news.demon.net!demon!r832.demon.co.uk!r832.demon.co.uk!Richard From: Richard Steele Newsgroups: bionet.general,bionet.microbiology,talk.origins,bionet.biophysics,bionet.molbio.evolution,comp.ai.genetic,sci.physics,alt.life.universe.everything Subject: Re: conclution on the question of evolution (He Feng) Date: Tue, 6 May 1997 07:22:56 +0100 Organization: mayonnaise Maker Distribution: world Message-ID: References: <862823475.27196@dejanews.com> <5kl491$ea3@mailgate.lexis-nexis.com> NNTP-Posting-Host: r832.demon.co.uk X-NNTP-Posting-Host: r832.demon.co.uk MIME-Version: 1.0 X-Newsreader: Turnpike Version 3.02 Lines: 21 Xref: biosci bionet.general:26703 bionet.microbiology:9866 talk.origins:311417 bionet.biophysics:2982 bionet.molbio.evolution:6074 comp.ai.genetic:11120 sci.physics:211542 Christopher C. Wood wrote > >We only have one environment to study. I believe current research >shows a small handful of hundreds of millions of years between the end >of the planet's bombardment/formation, and the first cells. If a >process that takes 200,000,000 years can be called "springs out", then >the answer is "so far, yes." etc ... >Chris >-- >Speaking only for myself, of course. And speaking to an awful lot of people who *don't* want to know! Clear all the ruddy crossposts off this thing!!! -- Richard - Whose nice comfy chair by the fire has a new squeak ! From owner-biophysics@net.bio.net Mon May 05 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!news.mathworks.com!news.maxwell.syr.edu!disgorge.news.demon.net!demon!dispatch.news.demon.net!demon!r832.demon.co.uk!r832.demon.co.uk!Richard From: Richard Steele Newsgroups: bionet.general,bionet.microbiology,talk.origins,bionet.biophysics,bionet.molbio.evolution,comp.ai.genetic,sci.physics,alt.life.universe.everything Subject: Re: conclution on the question of evolution (He Feng) Date: Tue, 6 May 1997 07:20:53 +0100 Organization: mayonnaise Maker Distribution: world Message-ID: References: <862823475.27196@dejanews.com> <5NMoH4AapcbzEwLR@r832.demon.co.uk> <336DC718.28D1@biology.bu.edu> NNTP-Posting-Host: r832.demon.co.uk X-NNTP-Posting-Host: r832.demon.co.uk MIME-Version: 1.0 X-Newsreader: Turnpike Version 3.02 Lines: 29 Xref: biosci bionet.general:26702 bionet.microbiology:9865 talk.origins:311416 bionet.biophysics:2981 bionet.molbio.evolution:6073 comp.ai.genetic:11119 sci.physics:211541 Chris Colby wrote >Richard Steele wrote: > >> >Hi! I am the high school student who raised the question on evolution. >> >Since then I've received quite a number of responses. > >> I'll bet even *more* told you to bog off. Can't you take a hint? > >It's a high school kid curious about evolution. Do you have to be such >an asshole? Is it bad to foster kid's interest in biology and science? Of course not. But he might pay attention to where he's posting all this luvverly stuff! > >> >I'd like to thank all those who have kindly shared their knowledge and >> >thoughts with me. > >> Another tosser on line > >He's thanking people for responding, and you call him a tosser? Oh, the >irony. Get bent Richard. Hey wuss!! Just sod off out of my ng ok? -- Richard - Whose nice comfy chair by the fire has a new squeak ! From owner-biophysics@net.bio.net Mon May 05 23:00:00 1997 Path: biosci!daresbury!uninett.no!nntp.uio.no!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!howland.erols.net!swrinde!ihnp4.ucsd.edu!munnari.OZ.AU!bunyip.cc.uq.edu.au!newshost.gu.edu.au!usenet From: Tony Lu Newsgroups: bionet.biophysics Subject: Laser Photobiology Date: Sun, 04 May 1997 14:07:32 -0700 Organization: Griffith University, Brisbane, Australia Lines: 9 Message-ID: <336CFA94.3B8@kraken.itc.gu.edu.au> NNTP-Posting-Host: jun.me.gu.edu.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (Win16; I) Hi, I am interested in knowing how lasers may affect biological oraganism. Does any one know any applications of lasers in genetic engineering? Or how lasers affect living cells? Thanks. From owner-biophysics@net.bio.net Mon May 05 23:00:00 1997 Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!feed1.news.erols.com!disgorge.news.demon.net!demon!dispatch.news.demon.net!demon!cawley.demon.co.uk!cawley.demon.co.uk!alec From: Alec Cawley Newsgroups: bionet.general,bionet.microbiology,talk.origins,bionet.biophysics,bionet.molbio.evolution,comp.ai.genetic,sci.physics,alt.life.universe.everything Subject: Re: conclution on the question of evolution (He Feng) Date: Tue, 6 May 1997 22:04:05 +0100 Organization: Home Distribution: world Message-ID: <7Dk3VDAFz5bzEwaG@cawley.demon.co.uk> References: <862823475.27196@dejanews.com> <5kl491$ea3@mailgate.lexis-nexis.com> NNTP-Posting-Host: cawley.demon.co.uk X-NNTP-Posting-Host: cawley.demon.co.uk MIME-Version: 1.0 X-Newsreader: Turnpike Version 3.03 Lines: 26 Xref: biosci bionet.general:26711 bionet.microbiology:9874 talk.origins:311490 bionet.biophysics:2992 bionet.molbio.evolution:6085 comp.ai.genetic:11124 sci.physics:211576 It was said by Richard Steele that >Christopher C. Wood wrote > >> >>We only have one environment to study. I believe current research >>shows a small handful of hundreds of millions of years between the end >>of the planet's bombardment/formation, and the first cells. If a >>process that takes 200,000,000 years can be called "springs out", then >>the answer is "so far, yes." > >etc ... > >>Chris >>-- >>Speaking only for myself, of course. > >And speaking to an awful lot of people who *don't* want to know! > >Clear all the ruddy crossposts off this thing!!! Richard - you don't speak for everyone. Despite the crosspostedness of it, I find the sensible replies to a serious, if slightly naive, question of interest and value. As long as it isn't flaming, it isn't off topic in alue - but *you* are. Alec From owner-biophysics@net.bio.net Tue May 06 23:00:00 1997 Path: biosci!GOLD.CHEM.HAWAII.EDU!shuilin From: shuilin@GOLD.CHEM.HAWAII.EDU (Shuilin Niu) Newsgroups: bionet.biophysics Subject: cuvette holder Date: 6 May 1997 20:18:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi there: Could anybody please tell me where I can buy a compact thermoelectric cooled cuvette holder (holds 1.0 x 1.0 cm cuvette) with stirring capability? From owner-biophysics@net.bio.net Tue May 06 23:00:00 1997 Path: biosci!BIOSCI.CBS.UMN.EDU!cgross From: cgross@BIOSCI.CBS.UMN.EDU ("Carol Gross") Newsgroups: bionet.biophysics Subject: Moderating bionet.biophysics Date: 7 May 1997 07:58:43 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705071459.JAA04923@biosci.cbs.umn.edu> Reply-To: "Carol Gross" NNTP-Posting-Host: net.bio.net It has been proposed that the bionet.biophysics newsgroup be converted to a moderated newsgroup to avoid inappropriate postings which have been increasing on this group. The purpose of this notice is twofold: (1) to solicit volunteers to serve as moderators (without which the status of the group will not be changed). If you would be willing to serve as a moderator, please post a message to Carol Gross at cgross@biosci.cbs.umn.edu. The moderator position may be done on a rotational basis if several people are willing to act as moderator. (2) to determine whether the members of the group agree to such a conversion. If you endorse or oppose conversion to a moderated format, discussion should be conducted on the bionet.biophysics newsgroup. The deadline to volunteer for the moderator position is June 1, 1997. Carol Gross bionet.biophysics Discussion Leader **************************************************************** Carol Gross cgross@biosci.cbs.umn.edu Biophysics Internet Coordinator 612-625-5262 (voice) University of Minnesota 612-625-5780 (fax) Department of Biochemistry **************************************************************** From owner-biophysics@net.bio.net Tue May 06 23:00:00 1997 Path: biosci!UABDPO.DPO.UAB.EDU!bch0016 From: bch0016@UABDPO.DPO.UAB.EDU Newsgroups: bionet.biophysics Subject: Re: evolution?(please read) Date: 7 May 1997 08:52:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <3370C2BB.6873@uabdpo.dpo.uab.edu> References: <336FDA97.5D93@ultranet.com> Reply-To: bch0016@uabdpo.dpo.uab.edu NNTP-Posting-Host: net.bio.net crs wrote: > > John Gardner wrote: > > No FACT of evolution where one species has led > > to another. Change within species to an extremely limited > > degree yes, but nothing that would justify the _miraclulous_ > > transformation of one species into another. > > > > -- > > John Gardner A believer is believer, you never can convince him to change his mind. From owner-biophysics@net.bio.net Tue May 06 23:00:00 1997 From: ogr81@nospam.computan.on.ca (Rumple Stiltzski) Newsgroups: bionet.general,bionet.microbiology,alt.life.universe.everything,talk.origins,bionet.biophysics Subject: Re: LIFE vs DEATH (seeking answers) Date: Wed, 07 May 1997 16:09:40 GMT Message-ID: <3371a50f.4283648@news.computan.on.ca> References: <862647231.29844@dejanews.com> X-Newsreader: Forte Agent .99g/32.339 MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit NNTP-Posting-Host: 204.225.40.48 Lines: 46 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!torn!alpha.computan.on.ca! Xref: biosci bionet.general:26726 bionet.microbiology:9882 talk.origins:311656 bionet.biophysics:3003 hefeng@cz3.nus.sg wrote: >Hi! I am a high school student fascinated by the phenomena of life. I've >just discovered the wonder of Usenet so I've been posted a lot recently. >One thing I want to ask is that what exactly happens when life leaves a >living organism. Physically I don't see anything much has happened. We >still got roughly, if not exactly, the same amount of matter there, but >still something must have happened, since it's now dead! >So what is it? >If we consider life as an open system could we say that it's gone though >bifurcation and has 'jumped' to another state which is commonly called >'death'? >Life is certainly a strange phenomena. If we construct a system and throw >in some nutrients and a living organism, some time later we find two >organisms in the system. But if we throw in a DEAD organism, nothing will >happen no matter how long we wait. >Isn't it kind of odd? >Thank you. >---------- This question has been pondered for centuries, in many scientific (and otherwise, such as philosophy, theology) forums such as physics, anthropology, biology etc. and the solution to one question only leads to a myriad of others. I am not a scientist myself, you so can gauge how much credibility you would like to lend my theory. There is a form of light, as of yet undetected by human eye or aperatus, that dictates a large amount of survival instincts for life on our planets. This 'colour' varies in 'hue, depth, degree' etc. and depending on how certain parts of the brain respond, tell the biological creature what to do. For example, there is a hue of undetected light that tells a bird to fly south; another that drives the lemmings over cliffs, and another that breaths life into potential life. This light, like all light, is an energy, and it sparks the gap between existance and non-existance much the way a sparkplug does in a Renault. The knowledge of this light is very muddled at the moment, and the insinuations of it go by many different names. Myself, I call it God for lack of better word. Thanx for your time. Take care. >He Feng >e904952p@hjc.edu.sg > >-------------------==== Posted via Deja News ====----------------------- > http://www.dejanews.com/ Search, Read, Post to Usenet From owner-biophysics@net.bio.net Wed May 07 23:00:00 1997 Path: biosci!GEOCITIES.COM!gmed From: gmed@GEOCITIES.COM Newsgroups: bionet.biophysics Subject: FREE HOME PAGE Date: 8 May 1997 07:41:33 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: <33727203.5BD1@geocities.com> Reply-To: gmed@geocities.com NNTP-Posting-Host: net.bio.net FREE MONEY-SAVING, MONEY-MAKING HOMEPAGE! Links to other FREE SERVICES. For more information visit: HTTP://FREEDOMSTARR.COM/?SA3610301 From owner-biophysics@net.bio.net Wed May 07 23:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news-peer.sprintlink.net!news.sprintlink.net!Sprint!howland.erols.net!rill.news.pipex.net!pipex!server1.netnews.ja.net!server6.netnews.ja.net!str-ccsun!strath-cs!dcl-cs!bath.ac.uk!aber!not-for-mail From: prm@aber.ac.uk (Pedro Mendes) Newsgroups: bionet.metabolic-reg,bionet.cellbiol,bionet.molbio.proteins,bionet.biophysics,sci.bio.misc Subject: 1998 GRC on Macromolecular Organization and Cell Function Followup-To: bionet.metabolic-reg Date: 7 May 1997 09:30:10 GMT Organization: Univ Wales Aberystwyth Lines: 24 Distribution: world Message-ID: <5kpi32$1k0$1@infoserv.aber.ac.uk> NNTP-Posting-Host: pcjafc.dbs.aber.ac.uk Mime-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII X-Disclaimer: Warning - Sender not verified X-Newsreader: WinVN 0.99.7 Keywords: conference web page Xref: biosci bionet.metabolic-reg:1214 bionet.cellbiol:7272 bionet.molbio.proteins:10675 bionet.biophysics:3009 sci.bio.misc:8838 Gordon Research Conference on Macromolecular Organization and Cell Function September 13-17, 1998, Queen's College, University of Oxford Chaired by Douglas Kell (Aberystwyth) and John Wilson (Michigan) Full details (including application form) can be found on: http://gepasi.dbs.aber.ac.uk/grc98.html From the conference program: "The focus of the conference is on the functional organization of cellular components, and the critical role of this organization in metabolism and its regulation. [...] Conference participants have been especially sensitive to the fact that much of this organization may be destroyed, or at least grossly perturbed, when studies are performed in vitro. Thus, a persistent feature in the conference has been the cautious extrapolation of in vitro results to the in vivo condition, as well as development and application of techniques that allow one to study cellular structure and function in vivo. [...]" Check it at http://gepasi.dbs.aber.ac.uk/grc98.html From owner-biophysics@net.bio.net Thu May 08 23:00:00 1997 Path: biosci!CCIT.ARIZONA.EDU!AYool From: AYool@CCIT.ARIZONA.EDU (Andrea Yool) Newsgroups: bionet.biophysics Subject: Re: Moderating bionet.biophysics Date: 9 May 1997 08:54:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 46 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IINRRQB2CY938M8E@CCIT.ARIZONA.EDU> NNTP-Posting-Host: net.bio.net >It has been proposed that the bionet.biophysics newsgroup be converted to a >moderated newsgroup to avoid inappropriate postings which have been increasing >on this group. The purpose of this notice is twofold: > >(1) to solicit volunteers to serve as moderators (without which the status of >the group will not be changed). If you would be willing to serve as a >moderator, >please post a message to Carol Gross at cgross@biosci.cbs.umn.edu. The >moderator position may be done on a rotational basis if several people are >willing to act as moderator. > >(2) to determine whether the members of the group agree to such a conversion. >If you endorse or oppose conversion to a moderated format, discussion should >be >conducted on the bionet.biophysics newsgroup. > >The deadline to volunteer for the moderator position is June 1, 1997. ----------------------------------------------------------- Hi, Carol, I was planning either to volunteer, or to withdraw from the newsgroup. With the overwhelming redundancy of the recent set of postings on evolution, I've decided to withdraw. I don't think the postings fit into the category of inappropriate (as the sex and cash ones are) but nonetheless they have (for me) strayed beyond relevance to biophysics. Once the thermodynamic and equilibrium discussion points were made and all points of view were stated, the subject was sufficiently handled. Time to move on. Andrea Yool _____/\______________/\______________/\______________/\______________/\_____ /\ /\ /\ /\ /\ /\ /\ /\ /\ ____//\\____//\\____//\\____//\\____//\\____//\\____//\\____//\\____//\\____ \\// / \ \\// / \ \\// / \ \\// / \ \\// _____\/____/ \____\/____/ \____\/____/ \____\/____/ \____\/_____ ANDREA YOOL DEPTS OF PHYSIOLOGY AND PHARMACOLOGY UNIVERSITY OF ARIZONA ____________________________________________________________________________ From owner-biophysics@net.bio.net Thu May 08 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!news.mathworks.com!news.maxwell.syr.edu!disgorge.news.demon.net!demon!dispatch.news.demon.net!demon!r832.demon.co.uk!r832.demon.co.uk!Richard From: Richard Steele Newsgroups: bionet.general,bionet.microbiology,alt.life.universe.everything,talk.origins,bionet.biophysics,sci.physics,comp.ai.genetic,bionet.molbio.evolution Subject: Re: questions about embryos Date: Fri, 9 May 1997 13:17:40 +0100 Organization: mayonnaise Maker Distribution: world Message-ID: References: <862648806.30339@dejanews.com> <01bc5c22$f9da2d80$4fe386cd@default> NNTP-Posting-Host: r832.demon.co.uk X-NNTP-Posting-Host: r832.demon.co.uk MIME-Version: 1.0 X-Newsreader: Turnpike Version 3.02 Lines: 23 Xref: biosci bionet.general:26755 bionet.microbiology:9896 talk.origins:311986 bionet.biophysics:3013 sci.physics:211888 comp.ai.genetic:11154 bionet.molbio.evolution:6113 krhenry wrote >Please define what you mean by Embryo. Are you referring to the fertilized >egg? Or later, multicellular, stages? I haven't referred to embryos at all. I have, however, referred to the irresponsible list of groups that this moron has crossposted to. I did, once upon a long time, start my requests for removal of same, very politely. I don't feel polite any more. REMOVE THE FUCKING CROSSPOSTS YOU MORONS!!! -- Richard - Whose nice comfy chair by the fire has a new squeak ! From owner-biophysics@net.bio.net Thu May 08 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!news.mathworks.com!news-peer.sprintlink.net!news.sprintlink.net!Sprint!feed1.news.erols.com!disgorge.news.demon.net!demon!dispatch.news.demon.net!demon!bellona.demon.co.uk!bellona.demon.co.uk!malcolm From: Smudge Newsgroups: bionet.general,bionet.microbiology,alt.life.universe.everything,talk.origins,bionet.biophysics,sci.physics,comp.ai.genetic,bionet.molbio.evolution Subject: Re: questions about embryos Followup-To: alt.life.universe.everything Date: Fri, 9 May 1997 12:57:28 +0100 Organization: Izzy wizzy, let's get bizzy. Distribution: world Message-ID: References: <862648806.30339@dejanews.com> <01bc5c22$f9da2d80$4fe386cd@default> Reply-To: Smudge NNTP-Posting-Host: bellona.demon.co.uk X-NNTP-Posting-Host: bellona.demon.co.uk MIME-Version: 1.0 X-Newsreader: Turnpike Version 3.04 beta 3 Lines: 14 Xref: biosci bionet.general:26754 bionet.microbiology:9895 talk.origins:311984 bionet.biophysics:3012 sci.physics:211886 comp.ai.genetic:11153 bionet.molbio.evolution:6112 krhenry shook the scrabble bag and this fell out..... >Please define what you mean by Embryo. Are you referring to the fertilized >egg? Or later, multicellular, stages? Please go and find out what 'stop cross-posting' means and then do it. Thank you. -- Smudge Follow ups set to ALUE From owner-biophysics@net.bio.net Fri May 09 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!news-peer.sprintlink.net!news.sprintlink.net!Sprint!ais.net!news.texas.net!davej From: davej@sedona.net (Dave Jensen) Newsgroups: bionet.biophysics Subject: Bio-career Disussion Forum Date: Sat, 10 May 1997 09:31:23 -0700 Organization: Search Masters International Lines: 44 Message-ID: NNTP-Posting-Host: client44.sedona.net Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Yet Another NewsWatcher 2.3.4 A new, non-commercial career discussion forum is available for your browsing and posting interests. No commercial posts or spam are allowed on this news forum, and it is entirely moderated so as to make it 100% worthy of your contribution. There are hundreds of posts on dozens of topics of interest to anyone in the sciences. If you'd like, post your questions and find dozens of other people who may have had similar experiences and who you can network with or get some advice from. Already, networking subgroups have sprouted from the biotechnology section of this career discussion forum. Here are some topics JUST FROM THE LAST WEEK, and we have hundreds of archived posts: Career change to CRA Should you mention your advisor as a reference ? Best MBA for biotech Liberal Arts Positions? Scientist salaries? Temporary Placement Firms, any good? Multiple job offers, maybe? Salary trends Keeping the interview alive... One worried soon to be graduate. Changing jobs/employers Join our career discussion forum at http://cns.bio.com/hr/forum/ regards, Dave Jensen, Moderator From owner-biophysics@net.bio.net Sun May 11 23:00:00 1997 Newsgroups: bionet.biophysics Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!disgorge.news.demon.net!demon!dispatch.news.demon.net!demon!netcom.net.uk!nntpfeed.doc.ic.ac.uk!sunsite.doc.ic.ac.uk!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!ebi.ac.uk!news From: David Starks-Browning Subject: Parallel Programming Course at EMBL-Heidelberg Message-ID: Sender: starksb@sol19.ebi.ac.uk Date: Mon, 12 May 1997 21:03:29 GMT Organization: EBI - European Bioinformatics Institute X-Newsreader: Gnus v5.3/Emacs 19.34 Lines: 58 [please post and distribute] The Supercomputing Resource for Molecular Biology (SRMB), located at the European Molecular Biology Laboratory in Heidelberg, is offering training courses in Parallel Programming and High Performance Computing to European researchers in molecular biology. The next course will be held 8 - 14 June 1997. The course is an intensive and comprehensive introduction to parallel programming and high performance computing for European molecular biologists. Topics include: Heterogeneous distributed computing with PVM Message passing programming with MPI Data parallel programming with Fortran90/HPF Methodologies for designing parallel algorithms Performance modeling and scalability analysis Optimization for modern microprocessors Shared memory programming and parallelizing compilers Case studies from parallel applications in molecular biology There is considerable emphasis on hands-on experience through practical sessions. In particular, participants will write and execute their own parallel programs in PVM, MPI and Fortran90/HPF. The course is open to European researchers at the advanced post-graduate level who have research interests in molecular biology, including sequence analysis, image processing, structure refinement, protein design and molecular dynamics. Participants from EU countries will be supported by an EU HCM/ALSF grant. Residents of other EMBL member countries will be supported by funds from EMBL. Participants receive travel, accommodation and a subsistence allowance during their visit, as well as access to the computational facilities of the Laboratory. If you wish to attend you should fill out an online application form AS SOON AS POSSIBLE. Further information, with links to a detailed agenda and application forms, can be found at: ========================================================================= David Starks-Browning | starksb@ebi.ac.uk Technology Transfer Node Manager ! European Bioinformatics Institute ! Wellcome Trust Genome Campus ! tel: +44(0)1223-49 46 16 Hinxton, Cambridge CB10 1SD, UK ! fax: +44(0)1223-49 44 68 ========================================================================= From owner-biophysics@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!biosci!not-for-mail From: sunger@a.crl.com (Stefan Unger) Newsgroups: bionet.biophysics,bionet.molec-model Subject: CONF: 8th Cyprus Conf on New Methods in Drug Research (5/98) Date: 12 May 1997 16:27:20 -0700 Organization: BioSoftware Marketing Lines: 86 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Xref: biosci bionet.biophysics:3032 bionet.molec-model:1544 The 8th Cyprus Conference on New Methods in Drug Research Limassol, Cyprus May 4-9, 1998 Conference Executive: Chair: Alex Makriyannis (U. Conn. Storrs, USA) Committee: N. Castagnoli (USA), J.P. Devlin (USA), U. Hacksell (Sweden), L.H. Hurley (USA) and M. Abou-Gharbia (USA) For the past seventeen years, this classic event has served as the biennial opportunity for researchers and management executives to interact, exchange views and philosophies, preview cutting edge technologies in Drug Discovery and get a glimpse of things to come. The format is the traditional Gordon-style enhanced by the historical ambiance of Cyprus! Both the scientific papers and the interests of the participants are multidisciplinary and this mix, inevitably, results in innovative new approaches and collaborations. Half Day Technical Sessions: * New Roles for Synthetic Chemistry in Drug Design (Chair: Steve Hanessian (U. Montreal)) * Advances in Drug Receptor Research (Chair: Hendrik Timmerman (Amsterdam Center for Drug Research)) * The Use of Computational, Biophysical and Biochemical Methods (Chairs: Stephen Fesik (Abbott), Alex Makriyannis (U. Conn. Storrs)) * Ultra-High Throughput Technologies in Discovery and Development (Chair: Al Kolb (Packard Instrument)) * Micro- and Nanotechnologies in Measuring Biomolecular Interactions (Chair: Herman Gaub (Ludwig Maximilians Univ) * Computational-HTS Integration in Drug Discovery (Round Table Discussion) * Genomics-based Approaches in Discovery and Structure-Function Correlations (Chair: Casey Case (Tularik)) * Novel Design Approaches in Combating Drug Resistance (Chair: Lawrence Melvin (Amgen)) Conference Location Time and Registration: The Conference will take place from Monday, May 4th through Friday, May 9th 1998 at the five-star Hawaii Beach Hotel, Limassol, Cyprus. Attendance is restricted to 150 participants to encourage interaction. Registration fees (US $595 per person; US $395 for academics) includes attendance at all sessions and morning and afternoon breaks, but not meals. Local accommodations and air/ground travel expenses are the responsibility of the participant and can be arranged at the time of registration. Post conference tours are also available; please inquire. An important event, both as a refreshing midweek break and as an opportunity for casual and productive collegial interactions is the traditional Wednesday excursion to historical and archeological sites of interest followed by a memorable evening of Cypriot fare and entertainment. (Optional with separage charge). Sponsor: The European Federation of Medicinal Chemistry Contact: ARRT International, Inc., P.O. Box 1838 New Milford, CT 06776, USA Telephone: 860-355-5195 Facsimile: 860-355-5975 e-Mail: arrtintl@prodigy.net -- Stefan Unger, Ph.D. BioSoftware Marketing 4151 Middlefield Rd, Ste 109 Palo Alto, CA 94303-4743 From owner-biophysics@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!news-peer.sprintlink.net!news.sprintlink.net!Sprint!uunet!in3.uu.net!200.255.218.7!news.unisys.com.br!news From: "Lysandro Trotta Santos" Newsgroups: alt.paranet.metaphysics,alt.sci.physics.acoustics,alt.sci.physics.new-theories,alt.sci.physics.spam,bionet.biophysics,gac.physics.astronomy,harvard.physics.sps,sci.med.physics,sci.physics,sci.physics.accelerators,sci.physics.computational.fluid- Subject: MD Technology Date: 13 May 1997 01:28:26 GMT Organization: Smart Soft Co. Lines: 9 Message-ID: <01bc5f45$3a9bc340$4d04dcc8@harold> NNTP-Posting-Host: riopm02p12.unisys.com.br X-Newsreader: Microsoft Internet News 4.70.1155 Xref: biosci bionet.biophysics:3034 sci.med.physics:5473 sci.physics:212210 sci.physics.accelerators:2890 Hi! I'm from Rio de Janeiro, Brazil and I'm searching for some MiniDisk (MD) information. Anything!!! URLs, emails, newsgroups etc etc... Please, if someone could help me, send email to lysandro@uninet.com.br. I do appreciate your attention. Regards from Ipanema! Lysandro. From owner-biophysics@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!surfnet.nl!news-zh.switch.ch!swidir.switch.ch!news!news!nntp Newsgroups: bionet.biophysics Subject: unsubscribe ... Message-ID: <337836B8.5BD2@el.utwente.nl> From: Bas de Heij Date: Tue, 13 May 1997 11:39:04 +0200 Nntp-Posting-Host: pc35-75.unine.ch X-Mailer: Mozilla 3.0 (Win95; I) MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 1 and an other one bites the dust .... :-( From owner-biophysics@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news-peer.sprintlink.net!news.sprintlink.net!Sprint!howland.erols.net!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!news-feed1.tiac.net!uunet!in3.uu.net!152.163.170.17!newstf01.news.aol.com!audrey02.news.aol.com!not-for-mail From: regentag9@aol.com (Regentag9) Newsgroups: bionet.biophysics Subject: persistence length: a topological parameter of DNA et al. Date: 13 May 1997 03:35:08 GMT Organization: AOL http://www.aol.com Lines: 4 Message-ID: <19970513033400.XAA08578@ladder01.news.aol.com> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com Is it possible for a humble biologist to aquire a coherent explanation for this concept that does not invoke graduate level physics, yet does not "dumb down" and trivialize it? From owner-biophysics@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!HAVERFORD.EDU!jdepaula From: jdepaula@HAVERFORD.EDU (Julio de Paula) Newsgroups: bionet.biophysics Subject: unsubscribe Date: 13 May 1997 09:14:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net From owner-biophysics@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!news-out.communique.net!communique!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!disgorge.news.demon.net!demon!dispatch.news.demon.net!demon!netcom.net.uk!nntpfeed.doc.ic.ac.uk!sunsite.doc.ic.ac.uk!lyra.csx.cam.ac.uk!us1.rhbnc.ac.uk!yama.mcc.ac.uk!news.york.ac.uk!not-for-mail From: David Scott Newsgroups: bionet.biophysics Subject: Re: persistence length: a topological parameter of DNA et al. Date: Tue, 13 May 1997 14:01:09 -0700 Organization: University of York. York. Y01 5DD Sender: djs17@york.ac.uk Message-ID: <3378D695.32D5@york.ac.uk> References: <19970513033400.XAA08578@ladder01.news.aol.com> NNTP-Posting-Host: biolpc227.york.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) Lines: 39 Regentag9 wrote: > > Is it possible for a humble biologist to aquire a coherent explanation for > this concept that does not invoke graduate level physics, yet does not > "dumb down" and trivialize it? ok, here goes...... Persitance length is defined as the average projection of the end-to-end distance vector, r, on the first bond of the chain, in the limit of infinite chain length.....blah,blah,blah... So in English...... Persitance length is a measure of how long a polymer, such as DNA, perists in a certain direction. To give it a (useful) physical meaning, the direction of persitance is usually defined as being in the same direction as that of the first segment of the chain. So with DNA which is quite flexible, the persitance length is only a few 10's of nanometres, myosin is much greater, as it is stiffer. Thats the best I can do, however Biophysical Chemistry by Cantor and Schimmel (Freemann, 1980) gives a good, if mathematical introduction. I hope this helps.... Dave. -- ********************************* * Dr. David Scott * * Dept Biology * * University of York * * YORK * * UK * * * * phone +44 1904 432867 * * fax +44 1904 432860 * ********************************* From owner-biophysics@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!WWW.CYBERMEDIA-INC.COM!MAILER-DAEMON From: MAILER-DAEMON@WWW.CYBERMEDIA-INC.COM (Mail Delivery Subsystem) Newsgroups: bionet.biophysics Subject: Returned mail: User unknown Date: 13 May 1997 05:35:38 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 49 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705131239.IAA07902@www.cybermedia-inc.com> NNTP-Posting-Host: net.bio.net This is a MIME-encapsulated message --IAA07902.863527171/www.cybermedia-inc.com The original message was received at Tue, 13 May 1997 08:38:56 -0400 from host-207-53-124-214.mia.bellsouth.net [207.53.124.214] ----- The following addresses had permanent fatal errors ----- ----- Transcript of session follows ----- ... while talking to utep.el.utwente.nl.: >>> RCPT To: <<< 550 ... User unknown 550 ... User unknown --IAA07902.863527171/www.cybermedia-inc.com Content-Type: message/delivery-status Reporting-MTA: dns; www.cybermedia-inc.com Received-From-MTA: DNS; host-207-53-124-214.mia.bellsouth.net Arrival-Date: Tue, 13 May 1997 08:38:56 -0400 Final-Recipient: RFC822; heij_s@el.utwente.nl Action: failed Status: 5.1.1 Remote-MTA: DNS; utep.el.utwente.nl Diagnostic-Code: SMTP; 550 ... User unknown Last-Attempt-Date: Tue, 13 May 1997 08:39:31 -0400 --IAA07902.863527171/www.cybermedia-inc.com Content-Type: message/rfc822 Return-Path: biophys@net.bio.net Received: from mail.cybermedia-inc.com (host-207-53-124-214.mia.bellsouth.net [207.53.124.214]) by www.cybermedia-inc.com (8.8.5/8.7.3) with SMTP id IAA07900 for ; Tue, 13 May 1997 08:38:56 -0400 Date: Tue, 13 May 1997 08:38:56 -0400 From: biophys@net.bio.net Message-Id: <199705131238.IAA07900@www.cybermedia-inc.com> To: heij_s@el.utwente.nl Subject: Re: unsubscribe ... The newsletter does not exist. For detailed information on how to use the newsletter server, send a message to us and include only the word 'help' in the subject. --IAA07902.863527171/www.cybermedia-inc.com-- From owner-biophysics@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!MIDWAY.UCHICAGO.EDU!khouamed From: khouamed@MIDWAY.UCHICAGO.EDU (Khaled Houamed) Newsgroups: bionet.biophysics Subject: MOLECULAR NEUROBIOLOGY POSTDOCS U. CHICAGO Date: 13 May 1997 09:15:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 53 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Postdoctoral Positions in Molecular Neurobiology University of Chicago Postdoctoral positions are available to: 1) Perform structure / function studies on recombinant small conductance calcium activated potassium channels (SK channels) and, 2) Perform structure / function studies on recombinant G-protein coupled receptors members of family C (metabotropic glutamate receptors, calcium sensor, and GABA subB receptor). Recent Ph.D. graduates in Physiology, Pharmacology, Biochemistry, Neuroscience, or allied fields, with experience in cell physiology (patch clamp, oocyte voltage clamp, or fura measurements), or molecular biology techniques are invited to apply for these positions. Please send a current CV including a summary of relevant research experience and names and addresses of three referees to Dr. K. Houamed at the address below. Khaled Houamed Ph.D. Department of Anesthesia and Critical Care, Committees on Neurobiology and Cell Physiology The University of Chicago Medical Center 5841 S. Maryland Ave., Box 4028 Chicago, IL 60637 USA Tel # Secretary (773) 702-4055 Office (773) 702-7552 Laboratory (773) 834-0385 Fax # (773) 702-4791 The University of Chicago is an Affirmative Action, Equal Opportunity Employer. From owner-biophysics@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!feed1.news.erols.com!howland.erols.net!news2.digex.net!news.intercon.com!news5.digex.net!haven.umd.edu!news.umbc.edu!not-for-mail From: cweiss1@umbc.edu (Christopher Weiss) Newsgroups: bionet.biophysics Subject: Backbone bond angle and length data Date: 13 May 1997 15:42:59 -0400 Organization: University of Maryland, Baltimore County Lines: 18 Message-ID: <5lag83$5t2@umbc10.umbc.edu> NNTP-Posting-Host: umbc10.umbc.edu NNTP-Posting-User: cweiss1 I need protein backbone bond angle and bond length data. Not tortional angles. Angles and lengths needed: N-terminus H-N-H N-terminus H-N-C N-Calpha-H N-Calpha-C(carboxyl) O=C-Calpha O=C-N H-N-H (in peptide bond) C-N-Calpha C-terminus Calpha-C=O C-terminus Calpha-C-O C-terminus O=C-O Did I miss any? Thanks, Christopher From owner-biophysics@net.bio.net Tue May 13 23:00:00 1997 Newsgroups: bionet.biophysics Path: biosci!ihnp4.ucsd.edu!swrinde!cs.utexas.edu!feeder.chicago.cic.net!su-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!news.maxwell.syr.edu!news.new-york.net!actcom!news From: Shitka Gohding Subject: Free XXX X-Nntp-Posting-Host: jedi14.shani.net Message-ID: Sender: news@actcom.co.il (News) Organization: Mimimu LTD. Date: Tue, 13 May 1997 23:25:08 GMT Lines: 5 Goto: http://www.geocities.com/soho/lofts/5957/ Thanks. From owner-biophysics@net.bio.net Wed May 14 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!cpk-news-hub1.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!news.Stanford.EDU!nntp.Stanford.EDU!not-for-mail From: Andrew Fell Newsgroups: bionet.general,bionet.microbiology,alt.life.universe.everything,talk.origins,bionet.biophysics Subject: Re: LIFE vs DEATH (seeking answers) Date: Wed, 14 May 1997 18:00:43 -0700 Organization: Stanford University Lines: 36 Message-ID: <337A603B.6B7@netmatters.co.uk> References: <862647231.29844@dejanews.com> <3371a50f.4283648@news.computan.on.ca> Reply-To: ahfell@netmatters.co.uk NNTP-Posting-Host: facsnt2.stanford.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01Gold (Win95; I) Xref: biosci bionet.general:26810 bionet.microbiology:9943 talk.origins:312883 bionet.biophysics:3046 Rumple Stiltzski wrote: > > I am not a scientist myself, you so can gauge how much > credibility you would like to lend my theory. > There is a form of light, as of yet undetected by human eye or > aperatus, that dictates a large amount of survival instincts for life > on our planets. This 'colour' varies in 'hue, depth, degree' etc. and You can't see it or detect it, but it exists. Trust me. > depending on how certain parts of the brain respond, tell the > biological creature what to do. For example, there is a hue of > undetected light that tells a bird to fly south; another that drives > the lemmings over cliffs, and another that breaths life into potential > life. If you can't detect it, how does the brain respond to it? > This light, like all light, is an energy, and it sparks the gap > between existance and non-existance much the way a sparkplug does in a > Renault. Ah ha! A Renault driver! Now we're getting somewhere. > The knowledge of this light is very muddled at the moment, I would say muddled is an understatement. > the insinuations of it go by many different names. Myself, I call it > God for lack of better word. Because you can't see, hear, touch, taste, or feel it or measure it with any apparatus but you still think it exists despite any confirmatory evidence. Good definition of God. Andy the debunker From owner-biophysics@net.bio.net Wed May 14 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!chippy.visi.com!news-out.visi.com!newsfeed.direct.ca!torn!kone!news.ccs.queensu.ca!not-for-mail From: 4jch3@qlink.queensu.ca (Hesketh J Christian) Newsgroups: bionet.biophysics Subject: Ion Channel Web Page Date: 14 May 1997 20:43:18 GMT Organization: Queen's University, Kingston Lines: 20 Message-ID: <5ld856$ro7@knot.queensu.ca> NNTP-Posting-Host: qlink.queensu.ca X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] The ion channel web page has recently been updated. There is now a developing ion channel forum, links to the home pages and a publication list of several of the top ion channel researchers. A complete human voltage gated potassium channel sequence system has been installed as well as other pertinent topics in current ion channel research. Much work will be going into this page over the next month, so check back often. The URL for this site is: http://qlink.queensu.ca/~4jch3 Sincerely, --------------------------------------------------------------------------- J. Christian Hesketh Queen's University - Kingston, Canada Phone (613) 531-8048 e-mail: 4jch3@qlink.queensu.ca Web Page (Research in Ion Channels): http://qlink.queensu.ca/~4jch3 -Current research in ion channels with a biophysical perspective From owner-biophysics@net.bio.net Fri May 16 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!news-peer.sprintlink.net!news.sprintlink.net!Sprint!howland.erols.net!newsfeed.internetmci.com!news.ycc.yale.edu!news From: Richard Yu Newsgroups: bionet.xtallography,bionet.biophysics Subject: Protein crystal: Any suggestions for ATP heavy atom derivatives? Date: Fri, 16 May 1997 22:40:56 -0400 Organization: Yale University Lines: 9 Message-ID: <337D1AB8.41C6@laplace.csb.yale.edu> NNTP-Posting-Host: crick.csb.yale.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01Gold (X11; I; IRIX 5.3 IP22) CC: ryu Xref: biosci bionet.xtallography:3445 bionet.biophysics:3052 Greetings all: I'm wondering if any of you out there have ever used a heavy metal derivatized ATP for an ATP binding protein, or a funky derivative that happens to have an anomalously scattering atom. I've got one reference on 2'-iodoATP, but can't seem to find any others. Thanks, Rich From owner-biophysics@net.bio.net Fri May 16 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.biophysics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 May 1997 02:00:13 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705170900.CAA10695@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-biophysics@net.bio.net Sun May 18 23:00:00 1997 Newsgroups: bionet.biophysics Path: biosci!daresbury!uninett.no!nntp.uio.no!newsfeeds.sol.net!europa.clark.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!EU.net!CERN.ch!news From: "Chemical Dynamics" Subject: CURRICULUM-VITAE For Post-Doc in Biophysics X-Nntp-Posting-Host: tifrc4.tifr.res.in Message-ID: <01bc644c$9c1d76c0$0e1a909e@lakshmi.tifr.res.in> Sender: news@news.cern.ch (USENET News System) Organization: t i f r X-Newsreader: Microsoft Internet News 4.70.1155 Date: Mon, 19 May 1997 12:13:08 GMT Lines: 404 Name: Arvind Srivastava. Institution: Tata Institute of Fundamental Research E-Mail: asa225@tifrvax.tifr.res.in Objective: To apply for Post-doc in the field of Biophysics CURRICULUM-VITAE Name: Arvind Srivastava Date of birth: 13 August 1969 Institute: Tata Institute of Fundamental Research Homi Bhabha Road Bombay, 400 005, India. Correspondence address: Chemical Physics Group Tata Institute of Fundamental Research Homi Bhabha Road Bombay, 400 005, India Phone: 215 2971/215 2979 Ext. 2526. Fax: + 91 - (22) - 215 2110 E-mail: asa225@tifrvax.tifr.res.in Educational qualifications Degree/Exam. Year University % Marks/Division B. Sc. 1990 Purvanchal University Jaunpur, U.P. 67/1st Class M. Sc.(Chemistry) 1992 Banaras Hindu University, Varanasi. 68/1st Class Ph.D. (Chemistry) 1997 TIFR, Bombay. Expected to complete by Sept. 1997. Graduate Course works I have completed following courses as a part of my Ph.D. programme during 1992-1993. Theoretical Courses 1. Quantum Chemistry ; 2. Molecular Biophysics ; 3. Programming in C ; 4. Bio-Electrochemistry ; 5 . NMR Spectroscopy ; 6. Bio-inorganic Chemistry ; 7. Mathematical Methods ; 8. Fluorescence Spectroscopy and Applications. Experimental Projects 1 Production, Purification and Preliminary Characterization of Conserved region of "Myb" proteins; 2 Elementary steps in proton transport across membranes; 3 Rotational diffusion of Rose Bengal in various solvents. Average grade in the above courses was A-. Ph.D. programme After completing graduate course work I joined to Prof. G.Krishnamoorthy, for my Ph.D. in January, 1994. Ph.D. Title: Dynamics of Biological Systems. Technical Experiences: 1. I was involved in the installation of a Time-resolved fluorescence microscope which I have used for most of my experiments. 2. I have used a CW mode-locked, frequency doubled Nd-YAG laser driven dye (Rhodamine 6G and Pyridine 1) laser as the light source in all mytime-resolved fluorescence and anisotropy studies. I have used time-correlated single-photon- counting set up as the detection system. I am familiar with the operation and maintenance of this system. 3. All the data analysis were done using the program developed in C-language in our lab (by Prof. N. Periasamy). 4. I am familiar with cell culturing techniques. 5. I have practical and theoretical background in other physical techniques such as Steady- State Fluorescence Spectroscopy (Shimadzu and Flurolog 2), UV-VIS spectroscopy (Shimadzu UV-2100) and Circular Dichroism spectroscopy(Jasco-J600). Awards 1. Junior Talent Search Scholarship. 2. National Scholarship 3. Scholarship by the Tata Institute of Fundamental Research during 1992-1997. 4. Financial award from Department of Science and Technology towards travel to attend the XIIth International Biophysics Conference at Amsterdam, The Netherlands, during 11th Aug.- 16th Aug. 1996. 5. Financial support from Council of Scientific and Industrial Research towards travel to attend the XIIth International Biophysics Conference at Amsterdam, The Netherlands during 11th Aug.- 16th Aug. 1996. 6. Best poster award by Indian Biophysical Society in 1996. Talks given at various places: 1. University of Pune, Feb. 1993 in SERC School on Chemical and Biological Application of Laser. 2. All India Institute of Medical Science, New Delhi on 21 Feb. 1996. 3. Debye Institute, Department of Molecular Biophysics, Buy Ballot Laboratory,Utrecht University, Utrecht, The Netherlands, on 19th Aug.1996. 4. Department of Chemistry, Celestijnenlaan 200F, Leuven, Belgium on 20th Aug. 1996. 5. Inorganic Chemistry Division, Oxford University, UK on 22th Aug. 1996. 6. Biochemistry Department, Imperial College of Science, Technology and Medicine, London, UK on 25th Aug. 1996. List of Publications In Journals 1. Arvind Srivastava and S. Doraiswamy, Rotational diffusion of Rose Bengal, J. Chem. Phys. 1995, 103, 6197. 2. Anup Madan, P. K. Radha, Arvind Srivastava, Lakshmi C. Padhy and Ramakrishna V. Hosur, The DNA-binding domain of Drosophila melanogaster c-Myb undergoes a ultistate denaturation. Eur. J. Biochem. 1995, 230, 733. 3. Arvind Srivastava, Shanteri Singh and G. Krishnamoorthy, Rapid Transport of Protons across membranes by Aliphatic Amines and Acids, J. Phys. Chem. 1995, 99, 11302. 4. Arvind Srivastava and G. Krishnamoorthy, Time-Resolved Fluorescence Microscopic Estimation of Cytoplasmic Viscosity, Progress in Biophys. & Mol. Biol. 1996, 65, 192. 5. Madhavarao C. N. , Sauna Zuben E. ,Srivastava Arvind and Sitaram V.,Voids Underlying Dynamics in Proteins Membranes and Micelles, Eur. J. Biochem. 1997 (in press) 6. Arvind Srivastava and G. Krishnamoorthy, Cell Type and Spatial Location dependence of Cytoplasmic Viscosity Measured by Time-Resolved Fluorescence Microscopy, Arch. Biophys. and Biochem. 1997, 340, 159-167. 7. Arvind Srivastava and G. Krishnamoorthy, Time -Resolved Fluorescence Microscopy Could Correct for Probe Binding While Estimating Intracellular pH, Anal. Biochem. 1997, 249 (in press) 8. G. Krishnamoorthy and Arvind Srivastava, Intracellular Dynamics Seen Through Time- Resolved Fluorescence Microscopy, Current Science 1997, (in press) 9. M. M. G. Krishna, Arvind Srivastava, Ranjan Das and N. Periasamy,Translational and Rotational Dynamics of Surface Probes: Monte Carlo Simulations and Fluorescence Anisotropy Experiments. (Submitted) In conferences 1. Arvind Srivastava, Rotational Diffusion of Rose Bengal in Various Solvents, SERC School on Chemical and Biological Application of Laser Feb.1993. 2. G. Krishnamoorthy, Ishak Ahmed, Shantheri Singh, Hari Pada Maity and Arvind Srivastava, Rapid Transport of Protons across Membrane, Twelfth School on Biophysics of Membrane Transport, School Proceedings, Poland, May 4-13, 1994. 3. Arvind Srivastava and G. Krishnamoorthy, Estimation of Cytoplasmic Viscosity by Time- Resolved Fluorescence Microscopy, XIIIth Indian Biophysical Society meeting, All India Institute of Medical Science, New Delhi, India, Feb. 1996. 4. Arvind Srivastava and G. Krishnamoorthy, Time-Resolved Fluorescence Microscopic Estimation of Cytoplasmic Viscosity, XIIth International Biophysics Conference at Amsterdam, The Netherlands, during 11th Aug.-16th Aug. 1996 5. Arvind Srivastava and G. Krishnamoorthy, Intracellular pH estimation by Time-Resolved Fluorescence Microscopy, Symposium on Advances in Bio-Inorganic Chemistry during October 7-12, 1996, Tata Institute of Fundamental Research, Mumbai, India. 6. Arvind Srivastava and G. Krishnamoorthy, Estimation of Intracellular pH by Time -Resolved Fluorescence Microscopy, Indian Biophysical Society meeting during 9-12 Dec. 1996 at Indian Institute of Science, Bangalore,India. 7. G. Krishnamoorthy and Arvind Srivastava, Dynamic Fluorescence Microscopic Observations on Intracellular Mobility, during 9-12 Dec. 1996 at Indian Institute of Science, Bangalore, India. 8. M. M. G. Krishna, Ranjan Das, Arvind Srivastava and N. Periasamy, Translational and Rotational Dynamics of surface probes in Vesicles: Computer simulational and Fluorescence Microscopic experiments, during 9-12 Dec. 1996 at Indian Institute of Science, Bangalore, India. 9. Krishnamoorthy and Arvind Srivastava, Cell Membrane Seen Through Time-Resolved Fluorescence Microscopy, Thirteenth School on Biophysics of Membrane Transport, School Proceedings, Poland, May 11-18, 1997. Names & Addresses of Referees 1. Prof. G. Krishnamoorthy Chemical Physics Group Tata Institute of Fundamental Research Homi Bhabha Road, Mumbai 400 005, India Fax: 091-22-215-2110 E-mail: gk@tifrvax.tifr.res.in 2. Prof. N. Periasamy Chemical Physics Group Tata Institute of Fundamental Research Homi Bhabha Road, Mumbai 400 005, India Fax: 091-22-215-2110 E-mail: peri@tifrvax.tifr.res.in 3. Prof. S. Doraiswamy Chemical Physics Group Tata Institute of Fundamental Research Homi Bhabha Road, Mumbai 400 005, India Fax: 091-22-215-2110 E-mail : edorai@tifrvax.tifr.res.in 4. Prof. R. V. Hosur Chemical Physics Group Tata Institute of Fundamental Research Homi Bhabha Road, Mumbai 400 005, India Fax: 091-22-215-2110 E-mail: hosur@tifrvax.tifr.res.in Research experiences Instrumentation: I was involved in the installation of a time-resolved fluorescence microscope. This involved coupling of a fluorescence microscope with picosecond laser system. An inverted epifluorescence microscope (Nikon, Diaphot 300) was optically modified in order to steer the dye laser pulses into the optical path of the microscope. A CW mode-locked frequency-doubled Nd-YAG laser-driven dye (Rhodamine 6G) laser which generates 4-10 ps pulses was used .The laser beam was vertically polarized and the polarization was further restricted to the vertical position by a polarizer placed at the entrance to the microscope. The laser beam was guided to the objective lens. The set - up is capable of performing dynamic fluorescence measurements on samples with a spatial resolution of 0.5-1.0 mm and a time resolution of 50 ps. Also reliable data could be obtained from even very low levels (~102-103 molecules) of fluorophores. The fluorescence which was collected by the same objective lens was passed through cut-off filters, a Glan-Taylor polarizer and a pin hole assembly (placed at the image plane) and detected by a thermoelectrically cooled (Products for Research, USA) fast response photomultiplier (XP2020Q). Time resolution of the fluorescence signal was obtained by the time-correlated single-photon-counting experimental setup. Spatial resolution of the sample has been achieved either by the focussed laser beam or by the pin-hole kept at the image plane. Experimental: Time-Resolved Fluorescence Microscopic Estimation of Cytoplasmic Viscosity Information on the cell type and spatial location dependence of cytoplasmic viscosity would be very useful in understanding some of the processes occuring in the cell. For this purpose, the fluorescent dye Kiton Red (sulforhodamine B) was loaded into a variety of cells such as Swiss 3T3 fibroblast, human mononuclear cells, Sarcoma-180 tumor cell, Chinese hamster ovary cells, the plant cells Digitalis Lanata , stamen hair cells of Tradescantia and guard mother cells of Allium Cepa. Space-resolved measurements of cytoplasmic viscosity were carried out by using an experimental set-up wherein a picosecond laser system was coupled with an epifluorescence microscope. Fluorescence lifetime measurements showed that a large fraction ~70 % of Kiton Red was in the free form. Fluorescence anisotropy decay of Kiton Red in cells was analyzed by a two population (free and bound) model. The microviscosity of cytoplasm was estimated from the anisotropy decay kinetics of the free probe. Correlation time of free Kiton Red in cytoplasm shows significant dependence on the cell type. The following picture emerges from these data: (i) the cytoplasmic viscosity is not very far from that of bulk water for all the cells studied; (ii) the average value of cytoplasmic viscosity is larger in case of the plant cells when compared to those of the animal cells studied here; (iii) the positional variation in the value of cytoplasmic viscosity is larger in the case of the plant cells when compared to the animal cells and (iv) cytoplasmic viscosity inside the tumor cell Sarcoma-180 is not different from the normal mammalian cells studied here. Model studies in various simpler systems have shown that our observation of cell type dependence and spatial location dependence of the cytoplasmic viscosity could have the origin in the variation in one or more of the following conditions within the cells: (i) the level of structured water, (ii) the concentration of small and macromolecules, (iii) the hydrodynamic shape (compact or extended structure) of the molecules present and (iv) physical restrictions such as proximity of membrane, cytoskeletal network etc. Estimation of Intracellular pH by Time-Resolved Fluorescence Microscopy Optical measurement of intracellular pH measurement scores over other methods of pH measurements in the respect of facile measurement of real time variation of intracellular pH and the capability to map the spatial variation of pH within a single cell with the help of a fluorescence microscope. Although fluorescence intensity is the most convenient parameter for measurements, the convenience is offset by various uncertainties in the estimation of pH. Apart from the pH, the fluorescence intensity would also depend upon factors such as concentration of the probe, pathlength of the observation, light scattering , photobleaching and binding of the probe to macromolecules and membranes. Estimation of pH using fluorescence lifetime would eliminate the above mentioned drawbacks. We have used the fluorescent pH indicator SNARF-1 in estimating the intracellular pH in a variety of cells. A fluorescence microscope coupled with a picosecond laser system was used in these measurements. The principle of lifetime-based pH sensing lies in the estimation of the relative amplitudes of the lifetime components of the protonated and deprotonated forms of the probe which have distinctly different lifetimes. The main advantage of lifetime based sensing is the absence of complication due to changes in the pathlength of the observation, concentration of the probe, absence of light scattering and photobleaching. Also probe binding to the macromolecules and membranes could be taken into account while analyzing fluorescence decay curve. Our analysis show that probe binding could result in significant errors in pH-estimations based on steady-state intensity measurements such as the popular ratiometric methods. The origin of this difference lies in the higher value (~3 ns) of the lifetime of the predominant population of bound probe when compared to those of the free forms. In case where a fourth lifetime component ( ~ 0.1 ns) was observed, the pH value estimated by steady state was lower than that estimated by the renormalized amplitude. Study of Membrane Fluidity by Fluorescence Lifetime Distribution: The physical state of cell membranes is relevant in many functions of the cell and hence a knowledge on them is important for a complete description of cell physiology. In the past a variety of physical techniques have been employed for this purpose. In the present work we show the usefulness of lifetime distribution of the membrane probe Nile Red in obtaining information on the physical state of membranes. We have used the time-resolved fluorescence microscopic technique Liposomal membranes, planar supported membranes and membranes of various intact cells were probed under a variety of conditions. Fluorescence decay curves were analyzed by the Maximum Entropy Method (MEM) to obtain distribution of fluorescence lifetime of Nile Red in membranes. The width of lifetime distribution was used to characterize the heterogeneity of the membrane. In model systems such as DPPC and DMPC membranes, the width associated with MEM lifetime distributions of Nile Red was very sensitive to the physical state of the membrane. The width in the gel state was higher when compared to that in liquid crystalline state by about 5 fold. Temperature dependence of the width tracked exactly the physical state of lipid. The width was also sensitive to membrane composition such as the presence of cholesterol, membrane proteins and fatty acids. The following conclusions emerged from these data: (i) The width observed in the mammalian cells studied here were significantly larger when compared to those observed in the plant cells; (ii) the variation in the width observed in a single mammalian cell was about 2-3 fold which is significantly larger than the variation observed in the plant cells and (iii) the width observed in plasma membranes were larger when compared to the nuclear membrane in the mammalian cells. The last observation could be due to the fact that plasma membranes have higher level of cholesterol when compared to nuclear membranes Rapid Transport of Protons across membranes by Aliphatic acids and Amines The pH gradient generated across membranes can be dissipated efficiently by means of single turnover cycle of aliphatic amines and acids present in the membrane. The kinetics of this process was in the submillisecond timescale and was measured by creating pH gradients using temperature jump. The main principal behind this technique is the difference in the enthalpy changes associated with protonation equilibria of various pH buffers. Application of T- jump to a vesicular suspension having, for example Tris in the external aqueous phase and phosphate in the inner aqueous phase produces a DpH within ~2 ms. The decay of DpH (transmembrane proton transport) was measured fluorimetrically by the use of the fluorescent indicator Pyranine entrapped inside the vesicles. The observed DpH decay process was identified as due to rapid transmembrane movement of the neutral form of either the acid or the amine. The rate constant was estimated to be in the range of 1-6 x 103 s-1 in the case of aliphatic amines. Correlation of proton transport with water content of membranes Transmemembrane proton transport was measured in various lipid vesicles in order to correlate proton permeability coefficient with the hydration of the acyl chain. We have used the vesicles made up of pure saturated lipids DMPC, Egg PC, DPPC mixed lipid Soybean phospholipid and variety of unsaturated lipids like C14:1, C16:1, C18:1 , C20:1, C22:1 PC. The transmembrane permeability of proton was measured by giving a pH jump and monitoring the rate of change of internal pH with the help of fluorescent pH indicator pyranine trapped inside. The level of hydration of the acyl chain was estimated by measuring the fluores