From owner-biophysics@net.bio.net Wed Oct 01 23:00:00 1997 Path: biosci!daresbury!uninett.no!news.algonet.se!newsfeed.direct.ca!newsfeed.internetmci.com!204.238.120.130!jump.net!grunt.dejanews.com!not-for-mail Date: Wed, 01 Oct 1997 20:22:39 -0600 From: interhomeless@rocketmail.com Subject: Re: UNIQUE METHOD OF TREATMENT !!! Newsgroups: bionet.biophysics Message-ID: <875753787.24629@dejanews.com> Reply-To: interhomeless@rocketmail.com Organization: Deja News Posting Service References: <875577351.29342@dejanews.com> <34312538.77D1@york.ac.uk> X-Article-Creation-Date: Thu Oct 02 00:56:30 1997 GMT X-Originating-IP-Addr: 195.5.26.41 (opera.webber.net.ua) X-Http-User-Agent: Mozilla/2.0 (compatible; MSIE 3.02; Windows 95) X-Authenticated-Sender: interhomeless@rocketmail.com Lines: 17 In article , David Scott wrote: > > bollocks is the phrase that springs instantly to mind...... I have the results of serious scientific experiments and a concrete results of treatment of people !!! But your have only your crappy newspaperese... Without the best regards, Valery Kotul. -------------------==== Posted via Deja News ====----------------------- http://www.dejanews.com/ Search, Read, Post to Usenet From owner-biophysics@net.bio.net Wed Oct 01 23:00:00 1997 Path: biosci!HOTMAIL.COM!rka2 From: rka2@HOTMAIL.COM (Rka2@hotmail.com) Newsgroups: bionet.biophysics Subject: (none) Date: 2 Oct 1997 03:23:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 43 Sender: daemon@net.bio.net Distribution: world Message-ID: <53588968@ccw.net> NNTP-Posting-Host: net.bio.net To: rka2@hotmail.com From: rka2@hotmail.com Subject: Simple Reply-to:rka2@hotmail.com Comments: Authenticated sender is Received: from hotmail.com (hotmail.com [000.000.000.000]) by hotmail.com (0.0.0./0.0.0.) with SMTP id AAA000000 for ; Mon, 29 Sep 1997 13:22:47 -0500 (EST) Message-Id: 0000000000.AAA000@hotmail.com X-UIDL: 56245458865418336224131215259812 This may be the most important message of your life! 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From owner-biophysics@net.bio.net Wed Oct 01 23:00:00 1997 Path: biosci!BIOSCI.MBP.MISSOURI.EDU!baskin From: baskin@BIOSCI.MBP.MISSOURI.EDU (Tobias Baskin) Newsgroups: bionet.biophysics Subject: Re: UNIQUE METHOD OF TREATMENT !!! Date: 2 Oct 1997 06:05:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <875753787.24629@dejanews.com> NNTP-Posting-Host: net.bio.net Greetings, Valery Kotul wrote, > > I have the results of serious scientific experiments > and a concrete results of treatment of people !!! Sorry Valery, but placebos work 40% of the time. Fact is, some folks will recover if you give em scrabled eggs, served up with the right kind of sauce. Tobias Baskin From owner-biophysics@net.bio.net Wed Oct 01 23:00:00 1997 Path: biosci!agate!hammer.uoregon.edu!csulb.edu!logbridge.uoregon.edu!newsfeed.internetmci.com!206.229.87.25!news-peer.sprintlink.net!news.sprintlink.net!Sprint!howland.erols.net!recycled.news.erols.com!news.uni-c.dk!balder.adm.ku.dk!arnold!tlk From: Toke Lindegaard Knudsen Newsgroups: bionet.biophysics Subject: Breaking the barriers between biology, chemistry and physics. Date: Thu, 2 Oct 1997 15:25:32 +0200 Organization: University of Copenhagen Lines: 356 Message-ID: NNTP-Posting-Host: arnold.math.ku.dk Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: tlk@arnold I am submitting this very interesting article on behalf of Madhavendra Puri of the Bhaktivedanta Institute. Could anyone replying to this posting on the newsgroup please CC a copy to Madhavendra Puri at . That would be most helpful for me. Thank you! -Toke Lindegaard Knudsen. EVIDENCE THAT ATOMS BEHAVE DIFFERENTLY IN BIOLOGICAL SYSTEMS THAN OUTSIDE OF THEM Madhavendra Puri The Bhaktivedanta Institute E-mail: tumle@diku.dk A number of chemists report that plants, animals and human beings ROUTINELY TRANSMUTE MID-RANGE ELEMENTS (for example, potassium into calcium or magnesium into calcium) AS PART OF THEIR ORDINARY DAILY METABOLISM. These transmutations obey rules such as: Mg + O => Ca; K + H => Ca. This is revolutionary since, according to current physical theory, the energy levels required for such transmutations are billions of times higher than what is available in biological systems. Equally inexplicable fission reactions such as Ca => Mg + O; Ca => K + H are also reported. But revolutions in physics have repeatedly occurred, such as the quantum revolution in which the radical property of non-locality, previously considered impossible, is now accepted by physicists (see Aspect and Grangier 1986, Bransden and Joachain 1989, p.671-681, Chiao et al 1993, Squires 1990, p.173, Rae 1986, p.25-44, and Penrose 1990, p.369). What I am presenting here is not the "cold fusion" of Fleischmann and Pons which, as far as I know, lacks clear evidence of actual fusion. Even if the Fleischmann and Pons effect turns out to be actual fusion, it is only the fusion of isotopes of the lightest element hydrogen under special laboratory conditions which is quite different from the UNEQUIVOCAL FUSION AND FISSION OF MID-RANGE elements found in biological transmutation reports. Now let us examine the evidence for biological transmutation. Crabs, shellfish and crayfish have shells made largely of calcium. A crab 17 cm by 10 cm has a shell weighing around 350 grams. Periodically these animals shed their shell and create a new one. This is called molting. When molting, a crab is very vulnerable and hides away from all other creatures so it can not get calcium by preying on other creatures. According to French chemist C. Louis Kervran of the Conseil d'Hygiene in Paris, sea water contains far too little calcium to account for the rapid production of a shell (the calcium content of sea water is about 0.042% and a crab can form a new shell in little more than one day). If the entire body of a crab is analyzed for calcium, it is found to contain only enough calcium to produce 3% of the shell (even taking into account the calcium carbonate stored in the hepato-pancreas just before molting). Even in water completely devoid of calcium, shellfish can still create their calcium-bearing shells as shown by an experiment performed at the Maritime Laboratory of Roscoff: "A crayfish was put in a sea water basin from which calcium carbonate had been removed by precipitation; the animal made its shell anyway." (Kervran 1972, p.58) "Chemical analysis made on animals secreting their shells has revealed that calcium carbonate is formed on the outer side of a membrane although on the opposite side of the membrane, where matter enters, there is no calcium. This fact has left specialists perplexed." (Kervran 1972, p.58) Sea water contains a sufficient amount of magnesium to form a shell if we accept Kervran's proposition that crabs routinely transmute magnesium into calcium; Mg + O => Ca. It would be interesting to put a crayfish in water devoid of both calcium and magnesium and see if it can still create its shell. Normal egg shells produced by hens contain calcium. Kervran (1972, p.41) reported an experiment in which hens were confined in an area in which there was no source of calcium and no calcium was present in their diet. The calcium deficiency became clearly manifested after a few days when the hens began to lay eggs with soft shells. Then purified mica (which contains potassium) was given to the hens. Kervran (1972, p.41) described what then transpired: "The hens jumped on the mica and began scratching around it very rapidly, panting over it; then they rested, rolling their heads on it, threw it into the air, and began scratching it again. The next day eggs with normal shells (weight 7 grams) were laid. Thus, in the 20 hours that intervened, the hens transformed a supply of potassium into calcium. ... An experiment of this kind, using the same mica, was undertaken with guinea-fowls over a period of forty days. The administering of the mica was suspended three times and each time a soft-shelled egg was laid ... ." One might suggest that the calcium in the egg shells was borrowed from the bones of the hens. But if this is true, why were soft eggs laid when the mica was withheld and normal eggs laid when mica was given to the hens? In order to avoid the conclusion that the hens transmuted potassium into calcium, one would have to show that mica somehow stimulates a metabolic pathway in which calcium is removed from the hen's bones and used in the production of the egg shells. This could be completely refuted by feeding the hens mica (and of course absolutely no calcium) for such a long period of time that all the calcium in their bones would have been completely exhausted. If after that time the hens still produce calcium-bearing egg shells, we must conclude that the calcium in the egg shells is not being taken from the bones. At that point, we seem to have no choice but to acknowledge the transmutation of potassium into calcium within the hens. Kervran (1972, p.52) described experiments performed in 1959 by the French government in the Sahara desert. The government was interested in determining the nutritional requirements of petroleum workers in the extreme heat prevalent in the desert. In the first experiment, conducted near a place called Ouargla, the total amount of magnesium ingested per day per man was measured and compared with the amount excreted. It was found that, on the average, each man daily excreted 117.2 milligrams of magnesium more than he ingested. Thus, each day, each man lost on the average 117.2 milligrams of magnesium. Now we must consider how much magnesium is on reserve in the human body: it turns out that the body is not able to mobilize more than 5000 milligrams of magnesium. Thus, at a daily loss of 117.2 milligrams, it is clear that after 50 days the bodies of the petroleum workers should have been completely depleted of magnesium. But the experiment was conducted for 180 days and each day each man excreted on the a verage 117.2 milligrams more than he ingested. The second experiment lasted for 240 days and was conducted near Tindouf which has a drier climate. This time each man excreted each day an average of 256 milligrams of magnesium more than he ingested. Under these conditions, after 20 days, each man should have been completely depleted of magnesium; but somehow they survived for 220 days thereafter. It seems difficult to avoid the conclusion that the human body is able to create magnesium. Biochemist H. Komaki of the University of Mukogawa in Japan reported that a number of different families of microorganisms such as Aspergillus niger and Saccharomyces cerevisiae create potassium during growth. (Komaki 1965, 1967) Kervran described a germination experiment using ryegrass seeds (type Rina) performed in 1971 by the Laboratory of the Societe des Agriculteurs de France (Kervran 1972, p.107). Out of an initial group of 2000 seeds, 1000 were set aside as a control batch and the other 1000 were germinated. The control batch weighed 2.307 grams before drying and 2.035 grams after drying. These 2.035 grams were analyzed and found to contain 3.02 milligrams of magnesium, 6.97 milligrams of potassium, 6.00 milligrams of calcium and 0.021 milligrams of copper. The magnesium, calcium and copper contents were determined by atomic absorption spectroscopy and the potassium content was determined by flame emission. The 1000 seeds to be germinated were germinated for 29 days in Petri dishes under a plastic sheet to insure that no dust could get in. Aside from 430 milliliters of Evian water, absolutely nothing else was supplied to the seeds during germination. 430 milliliters of Evian water was found to contain 10.32 milligrams of magnesium, 0.39 milligrams of potassium, 33.11 milligrams of calcium and 0.00 milligrams of copper. After the 29 day germination period, the plants were converted to ashes under high temperature and the ashes and residual Evian water in the Petri dishes were found to contain 3.20 milligrams of magnesium, 16.67 milligrams of potassium, 36.50 milligrams of calcium and 0.10 milligrams of copper. Before germination there were 6.97 milligrams of potassium in the seeds. During germination 0.39 milligrams of potassium were added to the growing plants (this came from the Evian water). If atomic nuclei can not be altered in biological systems, we expect that after germination there should be 6.97 + 0.39 = 7.36 milligrams of potassium in the plants and residual Evian water. But this was not the case. After germination the plants and residual Evian water were found to contain 16.67 milligrams of potassium. Thus 9.31 milligrams of potassium were apparently created during germination. Before germination there were 3.02 milligrams of magnesium in the seeds. During germination 10.32 milligrams of magnesium were added to the growing plants (this came from the Evian water). If atomic nuclei can not be altered in biological systems, we expect that after germination there should be 10.32 + 3.02 = 13.34 milligrams of magnesium in the plants and residual Evian water. But after germination the plants and residual Evian water were found to contain only 3.20 milligrams of magnesium. Thus 10.14 milligrams of magnesium were apparently destroyed during germination. Before germination there were 0.021 milligrams of copper in the seeds. During germination 0.00 milligrams of copper were added to the growing plants. Assuming that atomic nuclei can not be altered, we expect that after germination there should still be 0.021 milligrams of copper in the plants and residual Evian water. But it turned out that after germination the plants and residual Evian water were found to contain 0.10 milligrams of copper. Thus 0.079 milligrams of copper were apparently created during germination. Before germination there were 6.00 milligrams of calcium in the seeds. During germination 33.11 milligrams of calcium were added to the growing plants (from the Evian water). Assuming that nuclei can not be altered, we expect that after germination there should be 39.11 milligrams of calcium in the plants and residual Evian water. However, after germination the plants and residual Evian water were found to contain 36.50 milligrams of calcium. Thus 2.61 milligrams of calcium were apparently destroyed during germination. The following challenge can be made: no one knows how much potassium, calcium, magnesium and copper was in the seeds before they were germinated. It was assumed that the amounts of these elements was not significantly different from the amounts of these elements in the control batch. How do we know this is true? What should have been done is to start with a 100 grams of seeds, mix them around thoroughly, weigh out 50 batches of 2.000 grams each, randomly select 25 of these as control batches, determine the amounts of potassium, calcium, magnesium and copper in these batches and note the maximum variation in these elements among these batches. The remaining 25 batches can then be germinated and the plants analyzed for element content. In this way we would have some measure of the variation among different batches (both germinated and control). On the positive side, it can be argued that since the seeds of the control and germinated batches were of the same type, the variation in element content between these two batches was not significant. Some support for this idea can be found in the data provided by chemist D. Long of the Michaelis Nutritional Research Laboratory in Harpenden, England. Long analyzed (using atomic spectroscopy) six batches of ryegrass seeds (each of which weighed 5.4 grams before drying) and discovered that the difference in potassium content between the batch containing the greatest amount of potassium and the batch containing the least amount of potassium was 0.054 milligrams of potassium per gram of dry seed weight. Similarly, the maximum difference in magnesium content was 0.033 milligrams per gram of dry seed weight, that of calcium was 0.091 milligrams per gram of dry seed weight, and that of copper was 1.19 micrograms per gram of dry seed weight. (Long 1971, p.7) Kervran proposed that the plants performed the following nuclear reactions: Mg + O => Ca; Ca => K + H. Kervran did not discuss the reaction involving copper. Based on experience derived from similar experiments, Kervran said that if the seeds are germinated in doubly-distilled water, the amount of transmuted material is much smaller and may fall within the range of experimental error and therefore not be significant. The reason for this is that each kind of plant is only able to transmute certain elements into certain other elements. Thus the experimenter must provide the plant with a certain amount of certain elements if he wants to observe a large amount of transmuted material. For germinating ryegrass seeds, Evian water is the perfect growth medium because it provides this particular kind of plant with the elements it needs. Kervran (1972, p.132) also described a series of experiments in which wheat and oat seeds were germinated "on porous ashless paper saturated with a fertilizing solution of salts dissolved in water. The solution was free of calcium." In the case of wheat (Roux Clair) there was 3.34 times more calcium in the plants than in the seeds; in the case of one kind of oats (Noire du Prieure) there was 4.16 times more calcium in the plants than in the seeds; in the case of another kind of oats (Panache de Roye) there was 4.51 times more calcium in the plants than in the seeds. The calcium content was determined by two independent methods (conventional chemical analysis and atomic absorption spectroscopy); both methods agreed closely. Kervran performed more than 20 such experiments, mostly on oat seeds. Kervran (1972, p.133) mentioned that the moon plays an important role in the production of calcium. The above huge increases in calcium were obtained in experiments in which the germination started at the new moon and stopped on the second full moon (after 6 weeks). This is an important consideration for those who attempt to duplicate these results. A lunar influence on the metabolic activity of various plants and animals was also reported by biologist Frank A. Brown. (Gauquelin 1969, p.131-133) D. Long questioned Kervran's methods of analysis. Long (1971, p.9) said that Kervran had made (in some of his earlier experiments) the mistake of comparing the ash weight of the control batch with the ash weight of the plants after germination. Kervran may have made this mistake in some of his earlier experiments but he did not do so in the ryegrass, wheat and oat germination experiments described above. In these experiments, he rightly compared the weight of the control batch with the weight of the seeds to be germinated. In other words, the weight comparison was done on the two batches of seeds before one batch was germinated. This is the correct procedure as acknowledged by Long himself. Long germinated ryegrass seeds in deionized water and reported that he was unable to observe a transmutation of elements. As discussed above, this is to be expected since without a sufficient input of certain elements, there is insufficient material to be transmuted. A more serious criticism is Long's claim that he corresponded with Kervran who advised him to germinate green lentil seeds (Leguminacae) in water containing certain minerals. Long reported that although he did this he was still unable to observe a significant transmutation of elements. But Long did not attempt to duplicate the best of Kervran's germination experiments, namely the ryegrass, wheat and oat experiments described above. I hope that many scientists will do these experiments and report the results to the scientific community. In the 1950s Pierre Baranger, a professor and the director of the Laboratory of Organic Chemistry at the Ecole Polytechnique in Paris, performed a large number of germination experiments and concluded that plants routinely transmute elements. Baranger did his experiments independently of Kervran. Baranger said: "My results seem impossible, but here they are. I took every precaution. I repeated the experiments many times. I made thousands of analyses for years. I had the results verified by third parties who did not know what I was investigating. I used several methods. I changed my experimenters. But there is no escape. We must submit to the evidence: plants transmute elements." (Michel 1959, p.82) I tried to get more information by writing letters to the Ecole Polytechnique, the Societe des Agriculteurs de France and the Agronomie Research National Institute, but I received no reply. In 1975 chemists O. Heroux and D. Peter of the Division of Biological Sciences of the National Research Council of Canada conducted a meticulous experiment with rats (Heroux and Peter 1975). They measured the amount of magnesium ingested through food, water (and even air) as well as the amount of magnesium excreted in the form of urine and feces over three periods of time: 69 days, 240 days and 517 days. In the case in which the rats were fed a diet in which the amount of magnesium ingested was less than the amount of magnesium excreted, it was expected that the total amount of magnesium in the body would decrease. In fact, long before the 517th day of the experiment it was expected that there would be zero magnesium in the body. However, when the rats were analyzed for total magnesium on the 517th day, each rat contained, on the average, 82 milligrams of magnesium. The method used to determine the amount of magnesium in the body, food, water, air, feces and urine was atomic absorption spectroscopy. Heroux and Peter verified the accuracy of their determinations by giving samples to two other laboratories (the Division of Chemistry at the National Research Council and the Department of Chemistry at McMaster University); both of these laboratories obtained essentially the same results as Heroux and Peter at the Division of Biology at the National Research Council. Finally, other methods were used (such as destructive neutron activation and spectrographic emission) and these methods yielded results very similar to those obtained using atomic absorption spectroscopy. I do not advise the replication of this experiment since it involved killing the rats in order to analyze their bodies for magnesium. Experiments involving animal killing are not required since there are many ways (as described above) to verify biological transmutation without such killing. Bibliography Albert, D. "Bohm's Alternative to Quantum Mechanics." Scientific American, May 1994, pages 32-39 Aspect, A. and Grangier, P. "Experiments on Einstein- Podolsky-Rosen-type Correlations with Pairs of Visible Photons." In Quantum Concepts in Space and Time (edited by R. Penrose and C. J. Isham). Oxford: Oxford University Press, 1986 Bohm, D. and Peat, F. Science, Order and Creativity. New York: Bantam Books, 1987 Bransden, B. and Joachain, C. Introduction to Quantum Mechanics. Essex: Longman Group U.K. Limited, 1989 Chiao, R., Kwait, P. and Steinberg, A. "Faster than light?" Scientific American, August 1993, pages 38-46 Darnell, J., Lodish, H. and Baltimore, D. Molecular Cell Biology. New York: W. H. Freeman and Co., 1990 Gauquelin, M. The Cosmic Clocks. London: Peter Owen, 1969 Heroux, O. and Peter, D. "Failure of balance measurements to predict actual retention of magnesium and calcium by rats as determined by direct carcass analysis." Journal of Nutrition, 1975, volume 105, pages 1157-1167 Kervran, C. Louis. Biological Transmutation. New York: Swan House Publishing Company, 1972 Komaki, H. "Sur la formation de sels de potassium par differentes familles de microorganismes dans un milieu sans potassium." Revue de Pathologie Comparee, Paris, September 1965 Komaki, H. "Production de proteines par 29 souches de microorganismes et augmentation du potassium en milieu de culture sodique, sans potassium." Revue de Pathologie Comparee, Paris, April 1967 Long, D. B. "Laboratory Report on Biological Transmutation." Monograph of the Henry Doubleday Research Society. Braintree, Essex, England, September 1971 Michel, A. "Un savant francais bouleverse la science atomique." Science et Vie, Paris, 1959, pages 81-87 Penrose, R. The Emperor's New Mind. New York: Vintage Press, 1990 Rae, A. Quantum Physics: Illusion or Reality? Cambridge: Cambridge University Press, 1986 Squires, E. Conscious Mind in the Physical World. Bristol: Adam Hilger, 1990 From owner-biophysics@net.bio.net Wed Oct 01 23:00:00 1997 Path: biosci!bloom-beacon.mit.edu!eru.mt.luth.se!news.algonet.se!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!prodigy.com!nntp.earthlink.net!usenet From: prismx@earthlink.net (Claire Haller) Newsgroups: bionet.neuroscience,bionet.biophysics,bionet.cellbiol Subject: SCIENCE-WEEK: This Week's Headlines (3 Oct 97) Date: Thu, 02 Oct 1997 14:22:14 GMT Organization: SCIENCE-WEEK Lines: 30 Message-ID: <610aqn$h5m@chile.earthlink.net> Reply-To: prismx@earthlink.net NNTP-Posting-Host: 153.36.161.133 X-Newsreader: Forte Free Agent 1.0.82 Xref: biosci bionet.neuroscience:20454 bionet.biophysics:3570 bionet.cellbiol:8173 Headlines in This Week's SCIENCE-WEEK (October 3, 1997) [complete news reports free at http://members.aol.com/sciweek] 1. International Concern Over Smuggling of Chlorofluorocarbons 2. Most Distant Galaxy Observed at Redshift 4.92 3. Electrochemical Growth Method for Creating Electrical Contacts 4. Relating Bond Energy to Catalysis in Catalytic Antibodies 5. A Synthetic Oligomer That Mimics Protein Folding 6. Possible Explanation for Levorotatory Amino Acids in Biology 7. A Block Copolymer of Collagen and Elastin in Marine Mussels 8. X-Ray Crystallography Evidence for Some Key Enzyme Structures 9. Analysis of Procapsid Assembly in Bacteriophage Virus 10. High Resolution Structure of the Nucleosome Core Particle 11. A New Theory of Myocardial Oxygenation in Fishes and Reptiles 12. Evidence for Self-Organization of Cell Microtubules 13. Mechanism of Action of Tumor Suppressor Protein p53 14. An Analysis of Action Potential Dynamics at Neuron Terminals 15. Identification of Gene Responsible for Mediterranean Fever 16. The Prospects for Gene Therapy 17. A New Drug Against Influenza Virus Infection 18. Chromosome Mis-Segregation in Alzheimer's Disease 19. High Concentrations of Cancer Inhibitor in Broccoli Sprouts 20. A Counter-Argument Against Pessimism in Cancer Research The Editors SCIENCE-WEEK A Free Weekly Digest of the News of Science prismx@earthlink.net http://members.aol.com/sciweek From owner-biophysics@net.bio.net Thu Oct 02 23:00:00 1997 Path: biosci!agate!howland.erols.net!news.maxwell.syr.edu!Supernews60!supernews.com!news3.sprint-canada.net!wolf.interlynx.net!not-for-mail From: Lars Thomsen Newsgroups: bionet.biophysics Subject: test Date: Sat, 04 Oct 1997 14:48:29 -0400 Organization: Interlynx Lines: 2 Message-ID: <34368F7C.9B51D423@interlynx.net> NNTP-Posting-Host: port29.hm.interlynx.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.03 [en] (Win95; I) asdf From owner-biophysics@net.bio.net Thu Oct 02 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!164.67.42.145!nntp.info.ucla.edu!134.87.113.1!news.bc.net!torn!nott!crc-news.doc.ca!srv1.drenet.dnd.ca!newshost.dres.dnd.ca!elaine.dres.dnd.ca!efulton From: efulton@dres.dnd.ca (Elaine Fulton) Newsgroups: bionet.biophysics Subject: electromagnetic rotation assay Date: Fri, 3 Oct 1997 14:00:47 UNDEFINED Organization: DRES Lines: 11 Message-ID: NNTP-Posting-Host: elaine.dres.dnd.ca Keywords: detection, identification, bacteria, virus, toxin, microorganisms X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #1] Several years ago an article appeared in New Scientist (15 May 93) on an electrorotation assay developed by Scientific Generics (Cambridge) and the University of Wales for the detection of microorganisms. The method used polystyrene beads coated with antibodies to captureand detect the target antigen at a sensitivity a million times greater than conventional techniques. the scientists involved in the development were Adrian Parton of Scientific Generics and Ron Pethig and Julian Burt of University of Wales. Does anyone have any further information on this development, have contacts with these scientists, or could anyone put me in touch with someone who could provide further information? Any and all information would be most appreciated. Thanks. Elaine From owner-biophysics@net.bio.net Fri Oct 03 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: Newsgroups: bionet.biophysics Subject: Madhavendra Puri's paper (Pentcho Valev) Date: 4 Oct 1997 09:49:52 +0100 Lines: 18 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <614vvg$spg@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk There is a strange tendency in all discussion groups to which I have contributed to leave any real problem unsolved. True, sometimes the problems are not clearly set; however, paradoxically, it is only in this case that an author can expect some critical or ironical comment. As soon as the author manages to clarify the issue, nobody seems to care anymore about the solution. The problem in Madhavendra Puri's paper "Evidence that atoms behave differently in biological systems..." is quite clearly set and deserves attention. What is bothering however is that there is no "microscopic" evidence - either theoretical or experimental. On one hand, a specific physical/chemical mechanism is looked for; on the other, the balance arguments are like those used in the beginning of the century when the principles of plant mineral nutrition were established. Perhaps Madhavendra would be so kind as to elaborate on the idea - how can we interprete these transmutations at the molecular level? Which enzymes are supposedly involved? Or the mechanism is not enzymatic at all? Pentcho Valev From owner-biophysics@net.bio.net Fri Oct 03 23:00:00 1997 Path: biosci!MAIN.INFOTEL.BG!steli From: steli@MAIN.INFOTEL.BG (Stelios Papaioanou) Newsgroups: bionet.biophysics Subject: rezeroing effect Date: 4 Oct 1997 10:23:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <1.5.4.32.19971004182329.0066a298@main.infotel.bg> NNTP-Posting-Host: net.bio.net Hi all, I'd be grateful to you if someone could explain me what is the "rezeroing effect". I read about it in Guyton's textbook of Physiology and there is a comparison between this and sleep. Thank you very much in advanced Stelios Papaioanou student of Medicine =20 Stara Zagora - Bulgaria e-mail:steli@main.infotel.bg =D3=F4=DD=EB=E9=EF=F2 Stelios Papaioanou e-mail:steli@main.infotel.bg From owner-biophysics@net.bio.net Fri Oct 03 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: Newsgroups: bionet.biophysics Subject: Experimental model of a possible bioenergetic cycle (Pentcho Valev) Date: 4 Oct 1997 13:31:05 +0100 Lines: 37 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <615cu9$87f@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk I would like to call attention to the following equilibrium system: low pH membrane-permeable-to-H+ only // impermeable-membrane-----------------X--- high pH The problem is: At the point X on the impermeable membrane, is there a transmembrane electrical force opposing movement of H+ down the concentration gradient or not? If there is not, an ATPsynthase placed at the point X could perform a concentration-driven ATP synthesis. As the system is at equilibrium, this would be a violation of the second law. An experimental model could consist in the following. 1. H+ is replaced with K+. 2. Two microelectrodes reversible for K+ (made of K amalgam) are placed on the two sides of the impermeable membrane, at the point X. If there is a current between the electrodes, this would mean that there is no transmembrane potential difference. Note that the current is accompanied by disappearance of K+ where the concentration is high and equivalent appearance where the concentration is low, i.e. the electrical work is fully analogous to the chemical work obtained as the pH gradient drives ATP synthesis. The experiment can even be simpler - no need to use an impermeable membane - the microelectrodes should simply be placed far enough from the permeable one: A 10 mM KCl M 1 mM KCl B where M is a membrane permeable only to K+ and A and B are distant points where the microelectrodes must be placed. I still believe that the question "Can ATP be synthesized in a system at equilibrium?" makes a lot of sense. I would be very happy to find people interested in the answer. Best regards, Pentcho Valev From owner-biophysics@net.bio.net Sat Oct 04 23:00:00 1997 Path: biosci!agate!howland.erols.net!newsfeed.direct.ca!news.uoregon.edu!newsfeed.orst.edu!128.101.135.70.MISMATCH!newshub.tc.umn.edu!newsstand.tc.umn.edu!pub-24-a-132.dialup.umn.edu!user From: scobi001@tc.umn.edu (Ron Scobie) Newsgroups: bionet.biophysics Subject: EEGDevice Date: Sat, 04 Oct 1997 22:25:33 -1000 Organization: Ron Scobie Enterprises Lines: 6 Message-ID: NNTP-Posting-Host: pub-24-a-132.dialup.umn.edu I am trying to find an EEG like device that a high school student might build or obtain(at low cost) for a science fair experiment. Would any of you be able to help? -- scobie@anoka.k12.mn.us From owner-biophysics@net.bio.net Sat Oct 04 23:00:00 1997 Path: biosci!agate!howland.erols.net!winter.news.erols.com!news From: drake@erols.com Newsgroups: bionet.biophysics Subject: Equation for plant growth Date: Sat, 04 Oct 1997 20:41:26 +0000 Organization: Erol's Internet Services Lines: 9 Message-ID: <3436A9F6.2AF1@erols.com> Reply-To: drake@erols.com NNTP-Posting-Host: col-as15s57.erols.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Received-On: 5 Oct 1997 01:36:31 GMT X-Mailer: Mozilla 2.02 (Macintosh; I; 68K) Have the results of any research been used to formulate an equation for plant growth? For example, the basic "water + CO2 + light energy = growth" formula; has there ever been a calculated ratio of CO2/light energy (x amount CO2/y amount of lumens, for example)? If such a ratio and equation do exist, have any been formulated that take into account temperature and nutrients? For instance, in the above equation iron would play a role. Any help with finding this information would be greatly appreciated. Thank you. From owner-biophysics@net.bio.net Sat Oct 04 23:00:00 1997 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!news-peer.sprintlink.net!news.sprintlink.net!Sprint!newsm.ibm.net!ibm.net!news.biu.ac.il!news.huji.ac.il!not-for-mail From: "Jonathan B. Marder" Newsgroups: bionet.biophysics Subject: Re: Donnan potential - reply to Jonathan Marder (Pentcho Valev) Date: Sun, 5 Oct 1997 12:33:14 +0200 Organization: Hebrew University Lines: 19 Message-ID: <617qdd$c68$1@news.huji.ac.il> References: <617n52$aj@mserv1.dl.ac.uk> NNTP-Posting-Host: marder.agri.huji.ac.il Mime-Version: 1.0 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: 7bit X-Newsreader: Microsoft Outlook Express 4.71.1712.3 X-MimeOLE: Produced By Microsoft MimeOLE V4.71.1712.3 RUMYM@BGEARN.ACAD.BG wrote in message <617n52$aj@mserv1.dl.ac.uk>... >... The infinity point has, by convention, a potential zero, so it follows that the point >near the right wall of the container has also a potential zero. If Ur is >the potential of the point far to the right and Uinf is the potential of >the reference infinity point, we have Ur - Uinf = 0, i.e. Ur = 0. > Pentcho, here is the crux of the problem. It is fine by me if you take the "infinity point" as potential zero, but you can only do that for ONE side of the membrane. Actually, in practice, since the space enclosed by a membrane is finite, there is no infinity point inside a membrane vesicle. I hope that this helps. Best regards, Jonathan From owner-biophysics@net.bio.net Sat Oct 04 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: Newsgroups: bionet.biophysics Subject: Donnan potential - reply to Jonathan Marder (Pentcho Valev) Date: 5 Oct 1997 10:37:38 +0100 Lines: 65 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <617n52$aj@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk Jonathan Marder wrote: RUMYM@BGEARN.ACAD.BG wrote in message <60tk8l$nk9@mserv1.dl.ac.uk>... < > 10 mM NaCl M 1 mM NaCl < > < >where M is a membrane permeable only to Na+. < > All references say that the system is electroneutral everywhere < except < >close to the membrane. However the potential difference between any two< >points in the two compartments is said to be about 60 mV. Measurements < >seem to support this. < The system is definitely *not* electroneutral. However, what happens far< from the membrane is that electrical fields are small i.e. there are < virtually no potential differences WITHIN a compartment (except close to< the membrane). The same goes for concentration. < I agree. > Let us try to assess the electrical potential at a point far to the < >right, near the right wall of the container. If a test positive charge < is < >moved from infinity to this point, no electrical work will be done as < the < >point is neutral and no electrical field will be crossed. < Agreed| < >Therefore, the < >potential at this point is zero. < NO - the potential *difference* is zero. < You are right, but this is the potential difference between the distant point far to the right and the reference point in infinity. The infinity point has, by convention, a potential zero, so it follows that the point near the right wall of the container has also a potential zero. If Ur is the potential of the point far to the right and Uinf is the potential of the reference infinity point, we have Ur - Uinf = 0, i.e. Ur = 0. >The same holds true for a point far to < >the left, near the left wall of the container. < Again, the potential *difference* is zero. < Analogously, Ul = 0, where Ul is the electrical potential of a point far to the left, near the left wall of the container. > Therefore, the potential difference between these two points distant< >from the membrane is zero, what contradicts the thermodynamic < prediction < >that the difference must be 60 mV. < > This is the whole problem, and I would ask again electrochemists to< >give their opinions. If the potential difference is indeed zero, the < >implications would be even difficult to imagine. < It would pose a huge problem. However, s you see from my approach, the < potential difference between two the points will be 60mV regardless of < distance from the membrane. < I think I have shown that Ur - Ul = 0, but maybe I do not see something. Please correct me if I am wrong. Best regards, Pentcho From owner-biophysics@net.bio.net Sat Oct 04 23:00:00 1997 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!newsfeed.internetmci.com!165.87.194.248!newsm.ibm.net!ibm.net!news.biu.ac.il!news.huji.ac.il!not-for-mail From: "Jonathan B. Marder" Newsgroups: bionet.biophysics Subject: Re: Donnan potential (Pentcho Valev) Date: Sun, 5 Oct 1997 09:36:44 +0200 Organization: Hebrew University Lines: 78 Message-ID: <617g2v$30s$1@news.huji.ac.il> References: <60tk8l$nk9@mserv1.dl.ac.uk> NNTP-Posting-Host: marder.agri.huji.ac.il Mime-Version: 1.0 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: 7bit X-Newsreader: Microsoft Outlook Express 4.71.1712.3 X-MimeOLE: Produced By Microsoft MimeOLE V4.71.1712.3 RUMYM@BGEARN.ACAD.BG wrote in message <60tk8l$nk9@mserv1.dl.ac.uk>... >(Sorry if this message has appeared more than once - my e-mail system >is down again) > >In reply to Jonathan Marder > >Jonathan, you deny the validity of both curves for equilibrium conditions >but do not suggest what the valid one should look like. It's very simple really. if the system is *at equilibrium* (as you stated), the electrochemical potential is (by definition) identical at all points - plot this as a horizontal line. However, this potential is the sum of concentration and electrical potential which would look something like this where C=concentration, E=electrical potential:- --outside--|- membrane-|--inside-- EEEEEEEEEEE|(E) (C)|CCCCCCCCCCC | (E) (C) | | (X) | | (C) (E) | CCCCCCCCCCC|(C) (E)|EEEEEEEEEEE Inside the membrane things get complicated wince ACTUAL concentrations are dependent on lipid solubility of the ion in question. Also, I have ignored the localized perturbations near the membrane surface. Actually we do not >need to know the shape of the curve near the membrane; the essential >problem is what happens to the electrical potential far from the membrane. > Let me describe the system in concrete numbers: > > 10 mM NaCl M 1 mM NaCl > >where M is a membrane permeable only to Na+. > All references say that the system is electroneutral everywhere except >close to the membrane. However the potential difference between any two >points in the two compartments is said to be about 60 mV. Measurements >seem to support this. The system is definitely *not* electroneutral. However, what happens far from the membrane is that electrical fields are small i.e. there are virtually no potential differences WITHIN a compartment (except close to the membrane). The same goes for concentration. > Let us try to assess the electrical potential at a point far to the >right, near the right wall of the container. If a test positive charge is >moved from infinity to this point, no electrical work will be done as the >point is neutral and no electrical field will be crossed. Agreed! >Therefore, the >potential at this point is zero. NO - the potential *difference* is zero. >The same holds true for a point far to >the left, near the left wall of the container. Again, the potential *difference* is zero. > Therefore, the potential difference between these two points distant >from the membrane is zero, what contradicts the thermodynamic prediction >that the difference must be 60 mV. > This is the whole problem, and I would ask again electrochemists to >give their opinions. If the potential difference is indeed zero, the >implications would be even difficult to imagine. It would pose a huge problem. However, s you see from my approach, the potential difference between two the points will be 60mV regardless of distance from the membrane. From owner-biophysics@net.bio.net Sat Oct 04 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: Newsgroups: bionet.biophysics Subject: Donnan potential (Pentcho Valev) Date: 5 Oct 1997 12:51:18 +0100 Lines: 20 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <617uvm$57c@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk Jonathan Marder wrote: Pentcho, here is the crux of the problem. It is fine by me if you take < the "infinity point" as potential zero, but you can only do that for ONE< side of the membrane. Actually, in practice, since the space enclosed by< a membrane is finite, there is no infinity point inside a membrane < vesicle. < Yes here is the crux of the problem. But why should we change the system? Let us solve first the problem for the case in which the right and left side of the membrane have "equal rights", i.e. for the system 10 mM KCl M 1 mM KCl where M is a membrane permeable only to K+. Do you agree that, at equilibrium, the potential difference between points symmetrical with respect to but distant enough from the membrane is zero? If yes, we will go immediately to the "vesicle" case - it is more complicated but more interesting of course. Pentcho From owner-biophysics@net.bio.net Sat Oct 04 23:00:00 1997 Path: biosci!rutgers!uwm.edu!newsspool.doit.wisc.edu!news.itis.com!not-for-mail From: Petr Kuzmic Newsgroups: bionet.biophysics Subject: Re: Madhavendra Puri's paper (Pentcho Valev) Date: Sun, 05 Oct 1997 15:57:48 -0500 Organization: BioKin Consulting Lines: 61 Message-ID: <3437FF4C.FD7A8C9E@biokin.com> References: <614vvg$spg@mserv1.dl.ac.uk> NNTP-Posting-Host: q27.itis.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.01 [en] (Win95; I) X-Priority: 3 (Normal) RUMYM@BGEARN.ACAD.BG wrote: > an author can expect some critical or ironical comment. Indeed. Speaking of critical and ironical comments, I attach a copy of a posting from sci.chem by a wickedly funny man named Uncle Al Schwartz... Petr Kuzmic (pkuzmic@biokin.com, http://www.biokin.com) ____________ Madhavendra Puri wrote: > > I would like to request that anyone who replies to this article on the > newsgroup kindly make a CC of the reply to me at tumle@diku.dk > That will be a big help. Thank you! > -Madhavendra Puri. > > EVIDENCE THAT ATOMS BEHAVE DIFFERENTLY IN > BIOLOGICAL SYSTEMS THAN OUTSIDE OF THEM > > Madhavendra Puri > The Bhaktivedanta Institute > E-mail: tumle@diku.dk > > A number of chemists report that plants, animals and human beings > ROUTINELY TRANSMUTE MID-RANGE ELEMENTS (for example, potassium into > calcium or magnesium into calcium) AS PART OF THEIR ORDINARY DAILY > METABOLISM. [SNIP] SPACE ALIENS EAT DWARF MUTANT HEIRESS AND HER DOG! RUN YOUR CAR ON WATER! MIRACLE HORSE RACING COMPUTER! BUNS OF STEEL! ABS OF STEEL! THINNER THIGHS IN 30 DAYS! DEAD PSYCHICS HOTLINE! WE KNEW YOU WOULD CALL! QUARTZ CRYSTALS! BEZOAR STONES! 5000 YOGIC FLOATERS TO CLEANSE THE EARTH OF ALL DISCORD! SQUARE THE CIRCLE! TRISECT THE ANGLE! LEARN THE MYSTERIES OF THE ANCIENTS! LAUNDRY RINGS CURE CANCER! APPLE CIDER VINEGAR CURES CANCER! MAGNETIC FIELDS CURE CANCER! ELVIS MATERIALIZES AND CURES CANCER! PRINCESS DI WAS SPACE ALIEN SENT TO EARTH TO CURE CANCER! Dr. Schund and his Institute for Ethnoanthropoesy has joined forces with Derrida Poswilly and the Institute for Institutional Analysis! All mathematics and science is at the verge of total obsolescence! This is the multi-level markieting extravaganza of the Millennium! Act now to lock in your territories for this fantastic and unstoppable overturn of all human civilization! Yada yada sis boom bah. -- Uncle Al Schwartz UncleAl0@ix.netcom.com ("zero" before @) http://www.ultra.net.au/~wisby/uncleal.htm (Toxic URL! Unsafe for children, Democrats, and most mammals) "Quis custodiet ipsos custodes?" The Net! From owner-biophysics@net.bio.net Sat Oct 04 23:00:00 1997 Path: biosci!BIOC.RICE.EDU!bartel From: bartel@BIOC.RICE.EDU (Bonnie Bartel) Newsgroups: bionet.biophysics Subject: faculty positions-Rice University Date: 5 Oct 1997 08:45:38 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: <3437B5B2.7FA9@bioc.rice.edu> NNTP-Posting-Host: net.bio.net Rice University Department of Biochemistry & Cell Biology Faculty Positions Applications are invited for two tenure-track faculty positions in biological sciences, one in cell and developmental biology and one in biophysics. Cell and developmental biology may encompass animal, microbial or plant systems, while biophysics areas include experimental, computational and theoretical approaches. Candidates must have completed a Ph.D. or M.D./Ph.D. program in a related field, have postdoctoral training, exhibit outstanding communication skills, and a record indicating significant potential in research and teaching. The successful candidate will be expected to develop and maintain a vigorous research program supported by extramural funding, and participate in graduate and undergraduate teaching. Emphasis in recruiting will be at the Assistant Professor level, but highly qualified candidates at other levels will also be considered. Review of submitted applications will commence on November 1, 1997, and continue until the positions are filled. Please send letter of application, curriculum vitae, summary of past research, and statement of future research plans, and arrange for four letters of reference to be sent to: Faculty Search Committee Department of Biochemistry & Cell Biology Rice University, MS-140 6100 Main Street Houston, TX 77005-1892 Rice University is an Equal Opportunity/Affirmative Action Employer; women and minority candidates are encouraged to apply. From owner-biophysics@net.bio.net Sat Oct 04 23:00:00 1997 Path: biosci!agate!howland.erols.net!newsfeed.internetmci.com!165.87.194.248!newsm.ibm.net!ibm.net!news.biu.ac.il!news.huji.ac.il!not-for-mail From: "Jonathan B. Marder" Newsgroups: bionet.biophysics Subject: Re: Donnan potential (Pentcho Valev) Date: Sun, 5 Oct 1997 14:43:33 +0200 Organization: Hebrew University Lines: 37 Message-ID: <61821p$isg$1@news.huji.ac.il> References: <617uvm$57c@mserv1.dl.ac.uk> NNTP-Posting-Host: marder.agri.huji.ac.il Mime-Version: 1.0 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: 7bit X-Newsreader: Microsoft Outlook Express 4.71.1712.3 X-MimeOLE: Produced By Microsoft MimeOLE V4.71.1712.3 RUMYM@BGEARN.ACAD.BG wrote in message <617uvm$57c@mserv1.dl.ac.uk>... >Jonathan Marder wrote: >Pentcho, here is the crux of the problem. It is fine by me if you take < >the "infinity point" as potential zero, but you can only do that for ONE< >side of the membrane. Actually, in practice, since the space enclosed by< >a membrane is finite, there is no infinity point inside a membrane < >vesicle. < > >Yes here is the crux of the problem. But why should we change the system? >Let us solve first the problem for the case in which the right and left >side of the membrane have "equal rights", i.e. for the system > > 10 mM KCl M 1 mM KCl > >where M is a membrane permeable only to K+. > Do you agree that, at equilibrium, the potential difference between >points symmetrical with respect to but distant enough from the membrane >is zero? No I do not! The convention you mention of "points at infinity have zero potential" cannot be applied to both sides of the membrane - that would be quite wrong. The purpose of the convention is to set a basline, not to ignore REAL differences in potential. To maintain a tenfold concentration of K+ at ANY separation (inc. infinity) requires a potential difference of 60mV. Pentcho, this is a non starter for me. I still cannot accept that there is any inherent contradiction in the accepted treatment of this topic. From owner-biophysics@net.bio.net Sun Oct 05 23:00:00 1997 Path: biosci!bloom-beacon.mit.edu!senator-bedfellow.mit.edu!dtuch From: dtuch@athena.mit.edu (David S Tuch) Newsgroups: bionet.biophysics Subject: extracellular conductivity Date: 5 Oct 1997 15:45:00 GMT Organization: Massachvsetts Institvte of Technology Lines: 4 Message-ID: <618cls$p6b@senator-bedfellow.MIT.EDU> NNTP-Posting-Host: altschuler.mit.edu I've been scouring the lit for any values on the DC electrical conductivity of brain tissue extracellular space. Any tips? Thanks. Dave Tuch dtuch@nmr.mgh.harvard.edu From owner-biophysics@net.bio.net Sun Oct 05 23:00:00 1997 Path: biosci!bloom-beacon.mit.edu!news-out.internetmci.com!newsfeed.internetmci.com!193.174.75.126!news-was.dfn.de!news-fra1.dfn.de!Cabal.CESspool!bofh.vszbr.cz!lyra.csx.cam.ac.uk!server1.netnews.ja.net!warwick!leicester!usenet From: "Dr E. Buxbaum" Newsgroups: bionet.biophysics Subject: Re: EEGDevice Date: Mon, 06 Oct 1997 09:46:06 -0700 Organization: University of Leicester (PCFS User) Lines: 55 Message-ID: <343915CE.2BFB@le.ac.uk> References: NNTP-Posting-Host: pc47.msb.le.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Win16; I) Ron Scobie wrote: > > I am trying to find an EEG like device that a high school student might > build or obtain(at low cost) for a science fair experiment. Building an amplifier for the bodies electrical signals (EEG, ECG and EMG) is relatively straight forward. Get a book on electronics and look under "instrumentation amplifier" to find the basic schematic. There are good ic's now for this purpose, for example Burr-Browns INA 118. This amplifier is usually followed by a low pass, to filter out mains hum, and an output buffer with variable gain. Solder two large coins to the inner core of screend cable and connect them to the inverting and non-inverting inputs of the amplifier. The screens of the cables are connected to the common input, as is a third coin via unscreend cable (the reference electrode). Smear the coins with a suitable conducting gel (for example KY) and fix them to the body (head for eeg) of your subject. The absolutely essential precondition is of course that all the circuits are powerd by battery, NOT by mains. Otherwise you may electrocute your subject! If you want to display the output of your amplifier on a PC (probably the easies and cheapest way to do it, a suitable A/D converter that connects to the printer port is in this months elector magazine), you must use a laptop powered by batteries. Mains operated equipment is definetly only for electronics engineers who know exactly what they are doing. There are realy nice experiments you can do with such a setup. Fix the measuring electrodes to right hand and left foot, the reference electrode to right foot and you get an ecg. Fix them across a muscle and you'l get an emg. With a different sort of electrode you can also show regular electrical signals in plants: Solder some silver wire to the cables as described above. Electrolytically cover the wires with AgCl. Then poke them into the plant (reference electrode goes into the soil). Try to do this with plants which react on touch (like Mimosa pudica)! May be you can also prove all those gardeners right who claim that plants grow better if you talk to them. The same setup can also be used to measure single nerve impulses in insects, like gras hoppers. For this you have to take a freshly killed insect and cut a window into the torax, to find the nerve which connects the wing position sensor with the central nerve system (your teacher should be able to help you with the anatomy, you'l also need a stereo microscope for the operation). The nerve is then rested on the two silver electrodes, the reference electrode is conneted to the insects body and a metal screen around the setup. Remember to keep the nerve moist with insect ringer. If you then carefully move around the wing, you should be able to observe a change in the discharge frequency of the nerve. The nerve has phasic-tonic characteristics, i.e. if you displace the wing, the initial frequency of the discharge is proportional to the degree of displacement, but after a while the frequency goes back to normal. You'l have to work fairly quickly though, as the nerve will stop to function some 2-3 h after the death of the insect. From owner-biophysics@net.bio.net Sun Oct 05 23:00:00 1997 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed4.bbnplanet.com!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!wuff.mayn.de!Cabal.CESspool!bofh.vszbr.cz!lyra.csx.cam.ac.uk!server1.netnews.ja.net!server5.netnews.ja.net!daresbury!not-for-mail From: Newsgroups: bionet.biophysics Subject: Donnan potential (Pentcho Valev) Date: 6 Oct 1997 13:39:33 +0100 Lines: 41 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <61am65$cea@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk I asked: > 10 mM KCl M 1 mM KCl >where M is a membrane permeable only to K+. < > Do you agree that, at equilibrium, the potential difference between < >points symmetrical with respect to but distant enough from the membrane < >is zero? < Jonathan Marder replied: No I do not| < The convention you mention of "points at infinity have zero potential" < cannot < be applied to both sides of the membrane - that would be quite wrong. < The purpose of the convention is to set a basline, not to ignore REAL < differences in potential. To maintain a tenfold concentration of K+ at < ANY separation (inc. infinity) requires a potential difference of 60mV.< < Pentcho, this is a non starter for me. I still cannot accept that there< is any inherent contradiction in the accepted treatment of this topic. < Jonathan, the problem is not scientific - it is psychological. It is quite normal that we reach different theoretical conclusions -in my opinion, the comparison with the infinity reference point makes more sense; you believe that the thermodynamic prediction is stronger. However it is not at all normal that the experimental verification is so easy and yet nobody wants to help. I am unlucky to live under conditions allowing no experimental activity; so I have been trying for years to convince people to do the experiment - one should only place two microelectrodes made of K amalgam at the two ends of the container. If the measured voltage is zero, thermodynamics is correct. If it is 60 mV, I would be right. (For this type of electrodes thermodynamics predicts zero voltage). The experiment would cost a specialized lab not more than half an hour, and the implications both thermodynamic and bioenergetic could be rather important. What should I do? I am even ready to pay (although I am by no means the reachest person in the world). Does anybody know what the proper behaviour is in cases like this? Best regards, Pentcho From owner-biophysics@net.bio.net Mon Oct 06 23:00:00 1997 Path: biosci!bloom-beacon.mit.edu!howland.erols.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!News.Toronto.iSTAR.net!News1.Vancouver.iSTAR.net!news.istar.net!nr1.ottawa.istar.net!not-for-mail From: newaccounts@stopat.com Newsgroups: bionet.biophysics Subject: Free FrontPage Web Site Date: Mon, 06 Oct 1997 21:25:40 PDT Organization: Email PLATINUM Lines: 19 Message-ID: <34399080.1@199.166.219.107> NNTP-Posting-Host: news2.connectmmic.net At StopAt you get your Web Page for Free. You also get an e-mail address and Web site hosting for a month. You may also update your page whenever and however you wish with Microsoft® FrontPage. Why are we being so generous?  The Web hosting industry is very competitive, and we want your business. So, while others charge up to $50 a month for hosting, we'll host your site for only $9.99 a month...or $99 a year (saving you 20% off the low monthly rate). For more information simply go to http://www.stopat.com/ or send an e-mail to newaccounts@stopat.com. StopAt Internet Customer Services *All names are registered trade marks of their respective owners. From owner-biophysics@net.bio.net Mon Oct 06 23:00:00 1997 Path: biosci!daresbury!uninett.no!news.maxwell.syr.edu!news-peer.sprintlink.net!news-pull.sprintlink.net!news-in-east.sprintlink.net!news.sprintlink.net!Sprint!169.226.1.109!rebecca!euclid.wadsworth.org!tivol From: tivol@news.wadsworth.org (William Tivol) Newsgroups: bionet.biophysics Subject: Re: Donnan potential - how far from the membrane? (Pentcho Valev) Date: 7 Oct 1997 21:29:02 GMT Organization: Wadsworth Center, NY Health Dept. Lines: 14 Distribution: bionet Message-ID: <61e9iu$ej9@euclid.wadsworth.org> References: <60qbjj$j7k@mserv1.dl.ac.uk> NNTP-Posting-Host: alcor.wadsworth.org X-Newsreader: TIN [version 1.2 PL2] RUMYM@BGEARN.ACAD.BG wrote: : (Bill, please, refer me to the book where you found the curve). P. R. Bergethon & E. R. Simons, Biophysical Chemistry: Molecules to Membranes. I extrapolated from and combined some of the figures from the later chapters of the book. I took their figure for the potential where there was an ex- cess of positive charge, inverted it for an excess of negative charge, and combined them. For the situation of the Na+-permeable membrane, the excess of + is on the right (low [NaCl]) and - is on the left (high [NaCl]). Yours, Bill Tivol From owner-biophysics@net.bio.net Mon Oct 06 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!206.229.87.25!news-peer.sprintlink.net!news.sprintlink.net!Sprint!uunet!in5.uu.net!amgen!news From: John Philo Newsgroups: bionet.biophysics Subject: Re: extracellular conductivity Date: Tue, 07 Oct 1997 13:09:47 -0700 Organization: Amgen Lines: 18 Message-ID: <343A970B.25EA@amgen.com> References: <618cls$p6b@senator-bedfellow.MIT.EDU> Reply-To: jphilo@amgen.com NNTP-Posting-Host: worf.amgen.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01GoldC-CIS0597c (Win95; U) To: David S Tuch David S Tuch wrote: > > I've been scouring the lit for any values on the DC electrical conductivity of brain tissue extracellular space. Any tips? Thanks. > > Dave Tuch > dtuch@nmr.mgh.harvard.edu That value should be well known by the people who do magnetoencephalography (i.e. the magnetic field equivalent to an EEG), since that conductivity affects the signals they see. Thus you may get to what you are looking for by searching the MEG literature. One name to try would be John Wikswo (Vanderbilt), an old friend who I know did a lot of theoretical work on propagation of EM waves through the brain as well as experimental MEG studies. Good luck, John Philo, Protein Chemistry, Amgen From owner-biophysics@net.bio.net Tue Oct 07 23:00:00 1997 Path: biosci!agate!howland.erols.net!newsfeed.nacamar.de!news-feed.inet.tele.dk!Cabal.CESspool!bofh.vszbr.cz!lyra.csx.cam.ac.uk!server1.netnews.ja.net!server5.netnews.ja.net!aber!not-for-mail From: "Douglas B Kell" Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.metabolic-reg,bionet.microbiology Subject: GRC Conference on Macromolecular Organisation and Cell Function 1998 Date: 7 Oct 1997 15:56:42 GMT Organization: University of Wales - Aberystwyth - Prifysgol Cymru Lines: 60 Message-ID: <01bcd339$9a460d10$2a187c90@pcjaeh> NNTP-Posting-Host: pcjaeh.dbs.aber.ac.uk X-Disclaimer: Warning - Sender not verified X-Newsreader: Microsoft Internet News 4.70.1155 Xref: biosci bionet.biophysics:3592 bionet.cellbiol:8204 bionet.metabolic-reg:1714 bionet.microbiology:11250 Dear colleagues, Together with my Co-Chair, Professor John Wilson of Michigan State University, East Lansing, MI, USA, I would like to alert you to the next Gordon Research Conference on "Macromolecular Organization and Cell Function." The conference will be held at Oxford University, UK, Sept. 13-18, 1998. A Gordon Conference with this theme has been held in alternate years since 1987. The focus of the conference is on the functional organization of cellular components, and the critical role of this organization in metabolism and its regulation. Initially, much of the attention was directed at enzymes, but this has greatly expanded over the years to include nucleic acids (e.g., targeting of mRNAs, assembly of complexes associated with transcription or translation), membranes, organelles, and delicate structures such as the nuclear matrix and cytoskeleton. Principles governing the assembly of such structures, as well as mechanisms by which these assemblies might be changed in response to altered metabolic or hormonal status, or by pharmaceutical intervention, are central issues in this conference. Conference participants have been especially sensitive to the fact that much of this organization may be destroyed, or at least grossly perturbed, when studies are performed in vitro. Thus, a persistent feature in the conference has been the cautious extrapolation of in vitro results to the in vivo condition, as well as development and application of techniques that allow one to study cellular structure and function in vivo. The result has been that this conference traditionally brings together scientists with interests ranging from biochemistry, cell biology, and molecular biology to theoretical formalisms, quantitative modelling, biophysics, physics, and (especially noninvasive) instrumentation. This truly interdisciplinary nature has consistently led to broadly based discussions and interactions which have been rated highly by those in attendance. We cordially invite you to participate in this next meeting. Full details of the very exciting speaker list, and links to the main GRC site (through whom applications to attend should be made), can be found on the conference's Web site, which we would suggest that you might bookmark: http://gepasi.dbs.aber.ac.uk/grc98.html Although we hope to have targeted known parties likely to be interested in participating, we be pleased if you could alert any of your other colleagues whose interests overlap those of the Conference. Kind regards and best wishes, Douglas Kell. ******************************************************************** *Douglas B. Kell Phone: +44 1970 622334 * *Edward Llwyd Building Fax: +44 1970 622354 * *Institute of Biological Sciences * *University of Wales, Email: dbk@aber.ac.uk * *Aberystwyth SY23 3DA, U.K. http://gepasi.dbs.aber.ac.uk/home.htm * ******************************************************************** ******************************************************************** From owner-biophysics@net.bio.net Wed Oct 08 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: Newsgroups: bionet.biophysics Subject: Donnan potential (Pentcho Valev) Date: 9 Oct 1997 15:47:17 +0100 Lines: 13 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <61iqpl$2us@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk I would like to inform the group that Herman Berendsen from Groningen kindly agreed to help me in measuring Donnan potential far from the membrane, in both directions, in different ways. If the potential is as thermodynamics predicts, I would apologize to the group for wasting so much time and archive space. However if the potential approaches zero with distance (as in the book Bill Tivol cited), I would ask bioenergeticists to take part in building a new theoretical foundation. This is not so dangerous - much more stable theoretical constructs have been demolished, replaced, developed etc. Aut inveniam viam aut faciam (I shall either find the road or make it). Best regards, Pentcho From owner-biophysics@net.bio.net Wed Oct 08 23:00:00 1997 Path: biosci!bloom-beacon.mit.edu!ai-lab!newsswitch.lcs.mit.edu!netnews.com!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!su-news-feed1.bbnplanet.com!news.bbnplanet.com!newsgate.tandem.com!uunet!in1.uu.net!hearst.acc.Virginia.EDU!murdoch.acc.Virginia.EDU!not-for-mail From: Wyling Com Newsgroups: bionet.biophysics Subject: National Interest Waiver and Green Card Date: Thu, 09 Oct 1997 13:38:33 -0700 Organization: University of Virginia Lines: 2 Message-ID: <343D40C9.4A76@consultation.net> NNTP-Posting-Host: bootp-65-91.bootp.virginia.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Win95; I; 16bit) Want to apply for green card through National Interest Waiver by Yourself? Send e-mail to wyling@consultation.net. From owner-biophysics@net.bio.net Wed Oct 08 23:00:00 1997 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed4.bbnplanet.com!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!prodigy.com!nntp.earthlink.net!usenet From: prismx@earthlink.net (Claire Haller) Newsgroups: bionet.neuroscience,bionet.biophysics,bionet.cellbiol Subject: SCIENCE-WEEK: This Week's Headlines (10 Oct 97) Date: Thu, 09 Oct 1997 16:20:49 GMT Organization: SCIENCE-WEEK Lines: 26 Message-ID: <61j0d7$shs@argentina.earthlink.net> Reply-To: prismx@earthlink.net NNTP-Posting-Host: 153.36.138.193 X-Newsreader: Forte Free Agent 1.0.82 Xref: biosci bionet.neuroscience:20500 bionet.biophysics:3595 bionet.cellbiol:8215 Headlines in This Week's SCIENCE-WEEK (October 10, 1997) [complete news reports free at http://members.aol.com/sciweek] 1. More Criticism of the Peer Review Process 2. A Cloning Moratorium by U.S. Biologists 3. Continuing Problems of Genome Patenting 4. Head of NIH Comments on Clinical Studies in Third World 5. Identification of Possibly the Most Luminous Star Known 6. A New Commercial Venture to Sell Asteroid Data 7. The Origin of Earth's Moon 8. A New Model for Earth's Magnetic Dynamo 9. Purple Bacteria, Quantum Physics, and Photosynthesis 10. Identification of a Mammalian Circadian Rhythm Gene 11. Identification of a Gene for a Key Vitamin D Enzyme 12. Mechanisms of Ligand-Gated Ion Channels 13. Mechanisms of Action of General Anesthetics 14. Identification of Macular Degeneration Gene 15. Alarm Over Chesapeake Bay Dinoflagellate Fish Parasite 16. Evidence for Apparent Smoke Resistance in Asian Arteries The Editors SCIENCE-WEEK A Free Weekly Digest of the News of Science prismx@earthlink.net http://members.aol.com/sciweek From owner-biophysics@net.bio.net Thu Oct 09 23:00:00 1997 Path: biosci!MAIL.CRYST.BBK.AC.UK!www From: www@MAIL.CRYST.BBK.AC.UK (Dave Houldershaw) Newsgroups: bionet.biophysics Subject: Protein Crystallography on the Web Date: 10 Oct 1997 11:52:16 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 32 Sender: daemon@net.bio.net Distribution: world Message-ID: <343E7A58.4A4F208D@mail.cryst.bbk.ac.uk> NNTP-Posting-Host: net.bio.net NEW Advanced Certificate in Protein Crystallography on the Web '97/'98 -------------------------------------------------------------------- Department of Crystallography, Birkbeck College, University of London Web site: http://px.cryst.bbk.ac.uk/ We are pleased to announce that we are now accepting registrations for this course. Protein crystallography is now a very multidisciplinary technique which has overlap with biochemistry, molecular biology, bioinformatics, biophysics and organic chemistry. The web course is therefore ideally suited for students of these disciplines who want to know in more detail (mainly non-mathematical) how structures are determined, how the quality of coordinates should be judged, and how crystallographic papers should be assessed. It will also appeal to those who envisage a further career in this exciting and rapidly expanding field, especially also in the pharmaceutical industry. This new course is delivered over the internet and makes use of innovative technologies including use of BioMOO for on-line tutorials, email discussion lists, and interactive course material. For more information please browse the URL given above. Please send any queries / requests for application forms to Claire Burton (c.burton@mail.cryst.bbk.ac.uk). The fee for students within the European Union is 260 pounds sterling, for non-EEA students the cost is 575 pounds sterling. Fee payments for overseas students should be made by cheque in pounds sterling drawn on a British bank. From owner-biophysics@net.bio.net Thu Oct 09 23:00:00 1997 Path: biosci!MAILGATE.SURFONAIR.CO.UK!agarcia From: agarcia@MAILGATE.SURFONAIR.CO.UK Newsgroups: bionet.biophysics Subject: Re: Donnan potential (Pentcho Valev) Date: 10 Oct 1997 09:48:24 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: <199710101649.RAB00096@mailgate.surfonair.co.uk> References: <617uvm$57c@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net how can I subscribe to this list and to bio-soft? thanks in advance. have a nice weekend > To: biophys@net.bio.net > From: > Subject: Donnan potential (Pentcho Valev) > Date: 5 Oct 1997 12:51:18 +0100 > Jonathan Marder wrote: > Pentcho, here is the crux of the problem. It is fine by me if you take < > the "infinity point" as potential zero, but you can only do that for ONE< > side of the membrane. Actually, in practice, since the space enclosed by< > a membrane is finite, there is no infinity point inside a membrane < > vesicle. < > > Yes here is the crux of the problem. But why should we change the system? > Let us solve first the problem for the case in which the right and left > side of the membrane have "equal rights", i.e. for the system > > 10 mM KCl M 1 mM KCl > > where M is a membrane permeable only to K+. > Do you agree that, at equilibrium, the potential difference between > points symmetrical with respect to but distant enough from the membrane > is zero? If yes, we will go immediately to the "vesicle" case - it is > more complicated but more interesting of course. > > Pentcho > > From owner-biophysics@net.bio.net Thu Oct 09 23:00:00 1997 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed4.bbnplanet.com!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.maxwell.syr.edu!news.he.net!news.dra.com!news2.interlog.com!news.interlog.com!not-for-mail From: Zanfir's Newsgroups: bionet.biophysics Subject: Who belives in The Philidalphia expirement? Date: Fri, 10 Oct 1997 00:26:19 -0400 Organization: Family's Computer Lines: 32 Message-ID: <343DAE6A.7C8BA823@interlog.com> NNTP-Posting-Host: ip226-40.cc.interlog.com Mime-Version: 1.0 Content-Type: multipart/mixed; boundary="------------B77FF0DD8BCE2092E9C95114" X-Mailer: Mozilla 4.02 [en] (Win95; I) This is a multi-part message in MIME format. --------------B77FF0DD8BCE2092E9C95114 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Hi, I was wondering if any one believes in the Philadelphia experiment and if they had info on it? if you don't know what im talking about its about the experiment Albert Einstein did with a ship, which was transported from one place to another. --------------B77FF0DD8BCE2092E9C95114 Content-Type: text/x-vcard; charset=us-ascii; name="vcard.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for Virgil Zanfir Content-Disposition: attachment; filename="vcard.vcf" begin: vcard fn: Virgil Zanfir n: Zanfir;Virgil adr: 1055A Forestwood Dr. #108;;;Mississauga;Ontario;L5C 2T8;Canada email;internet: zamfirv@interlog.com tel;work: 905-827-7913 x253 tel;fax: 905-827-9841 tel;home: 905-897-0652 x-mozilla-cpt: ;0 x-mozilla-html: FALSE version: 2.1 end: vcard --------------B77FF0DD8BCE2092E9C95114-- From owner-biophysics@net.bio.net Fri Oct 10 23:00:00 1997 Path: biosci!agate!howland.erols.net!newsfeed.internetmci.com!204.71.76.137!news.campus.mci.net!n-f-m From: "Bryan L. Brown" Newsgroups: bionet.biophysics Subject: Re: Equation for plant growth Date: Sat, 11 Oct 1997 01:18:33 -0000 Organization: CampusMCI Lines: 19 Message-ID: <61n278$k7q$1@news.campus.mci.net> References: <3436A9F6.2AF1@erols.com> NNTP-Posting-Host: s04-pm08.snaustel.campus.mci.net X-Newsreader: Microsoft Outlook Express 4.71.1712.3 X-MimeOLE: Produced By Microsoft MimeOLE V4.71.1712.3 None that I know of have been very widely accepted. There are a few speculative equations out there but they are all attempting to generalize across an extremely broad range of organisms and I doubt any equation could seriously attmept to do so. Most of the equations of the sort are organism specific or simply generalized models interested in making a particular point. If you do find what you think you're looking for, be wary. drake@erols.com wrote in message <3436A9F6.2AF1@erols.com>... >Have the results of any research been used to formulate an >equation for plant growth? For example, the basic "water + CO2 + >light energy = growth" formula; has there ever been a calculated ratio >of CO2/light energy (x amount CO2/y amount of lumens, for example)? > If such a ratio and equation do exist, have any been formulated >that take into account temperature and nutrients? For instance, in >the above equation iron would play a role. > Any help with finding this information would be greatly >appreciated. Thank you. From owner-biophysics@net.bio.net Fri Oct 10 23:00:00 1997 Path: biosci!agate!newsgate.duke.edu!nntprelay.mathworks.com!howland.erols.net!newspump.sol.net!newsspool.sol.net!newspump.wustl.edu!fas-news.harvard.edu!mlevin From: mlevin@login5.fas.harvard.edu (Michael Levin) Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.molbio.methds-reagnts,sci.engr.biomed,sci.med Subject: looking for slippery, permeable, artificial membrane Date: 11 Oct 1997 22:32:42 GMT Organization: Harvard University, Cambridge, Massachusetts Lines: 14 Message-ID: <61ouqa$p7h$1@news.fas.harvard.edu> NNTP-Posting-Host: login5.fas.harvard.edu Xref: biosci bionet.biophysics:3602 bionet.cellbiol:8227 bionet.molbio.methds-reagnts:61944 Can someone suggest where I can buy some of the following: a membrane (artificial) which is slippery and very smooth - so that tissues can grow and expand on it, and is also permeable to nutrients and other molecules. Perhaps something like this is used in organ or tissue culture, etc. I am basically trying to mimic the vitelline membrane covering the chicken embryo. Chick embryos can be cultured on the vitelline membranes, but it is a real pain in the butt to deal with the real one out of the egg. It would make things much easier if I could buy sheets of the stuff, and culture my embryos on it (laid over albumen). Please email mlevin@fas.harvard.edu if you have any ideas. Thanks! Mike Levin From owner-biophysics@net.bio.net Sat Oct 11 23:00:00 1997 Path: biosci!classic.msn.com!rcb5 From: rcb5@classic.msn.com ("Ronald Blue") Newsgroups: bionet.biophysics Subject: RE: looking for slippery, permeable, artificial membrane Date: 11 Oct 1997 18:39:23 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net This may work. Get a telflon pan, put a negative electrical charge on the pan and ground the positive. Pan should be on a rubber mat. If it does not work reverse the charge. Put me in as coinventor if it works and we will patent it. 50/50 split. Ron Blue http://www.neutronicstechcorp.com/private/ >>>>>>>>>>>>>> ---------- From: Michael Levin Sent: Saturday, October 11, 1997 6:32 PM To: biophys@net.bio.net Subject: looking for slippery, permeable, artificial membrane Can someone suggest where I can buy some of the following: a membrane (artificial) which is slippery and very smooth - so that tissues can grow and expand on it, and is also permeable to nutrients and other molecules. Perhaps something like this is used in organ or tissue culture, etc. I am basically trying to mimic the vitelline membrane covering the chicken embryo. Chick embryos can be cultured on the vitelline membranes, but it is a real pain in the butt to deal with the real one out of the egg. It would make things much easier if I could buy sheets of the stuff, and culture my embryos on it (laid over albumen). Please email mlevin@fas.harvard.edu if you have any ideas. Thanks! Mike Levin From owner-biophysics@net.bio.net Sat Oct 11 23:00:00 1997 Path: biosci!classic.msn.com!rcb5 From: rcb5@classic.msn.com ("Ronald Blue") Newsgroups: bionet.biophysics Subject: FW: looking for slippery, permeable, artificial membrane Date: 11 Oct 1997 18:45:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net If you want to watch the nervous system development have a modulating charge at 30 hertz, 10 hertz, etc... Ron Blue >>>>>>>>>>>> ---------- Sent: Saturday, October 11, 1997 9:42 PM To: biophys@net.bio.net; Michael Levin Subject: RE: looking for slippery, permeable, artificial membrane This may work. Get a telflon pan, put a negative electrical charge on the pan and ground the positive. Pan should be on a rubber mat. If it does not work reverse the charge. Put me in as coinventor if it works and we will patent it. 50/50 split. Ron Blue http://www.neutronicstechcorp.com/private/ >>>>>>>>>>>>>> ---------- From: Michael Levin Sent: Saturday, October 11, 1997 6:32 PM To: biophys@net.bio.net Subject: looking for slippery, permeable, artificial membrane Can someone suggest where I can buy some of the following: a membrane (artificial) which is slippery and very smooth - so that tissues can grow and expand on it, and is also permeable to nutrients and other molecules. Perhaps something like this is used in organ or tissue culture, etc. I am basically trying to mimic the vitelline membrane covering the chicken embryo. Chick embryos can be cultured on the vitelline membranes, but it is a real pain in the butt to deal with the real one out of the egg. It would make things much easier if I could buy sheets of the stuff, and culture my embryos on it (laid over albumen). Please email mlevin@fas.harvard.edu if you have any ideas. Thanks! Mike Levin From owner-biophysics@net.bio.net Sat Oct 11 23:00:00 1997 Path: biosci!cc.UManitoba.CA!gordonr From: gordonr@cc.UManitoba.CA (Richard Gordon) Newsgroups: bionet.biophysics Subject: Re: looking for slippery, permeable, artificial membrane Date: 11 Oct 1997 23:06:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Mike, Thin latex might do the trick. See: Harris, A.K. (1984). Tissue culture cells on deformable substrata: biomechanical implications. J. Biomech. Eng. 106(1), 19-24. Harris, A.K., P. Wild & D. Stopak (1980). Silicone rubber substrata: a new wrinkle in the study of cell locomotion. Science 208, 177-179. Lee, A.A., T. Delhaas, L.K. Waldman, D.A. MacKenna, F.J. Villarreal & A.D. McCulloch (1996). An equibiaxial strain system for cultured cells. Am. J. Physiol. 271(4 Pt 1), C1400-C1408. Best, -Dick Gordon >Can someone suggest where I can buy some of the following: a membrane >(artificial) which is slippery and very smooth - so that tissues can grow >and expand on it, and is also permeable to nutrients and other molecules. >Perhaps something like this is used in organ or tissue culture, etc. I am >basically trying to mimic the vitelline membrane covering the chicken >embryo. Chick embryos can be cultured on the vitelline membranes, but it >is a real pain in the butt to deal with the real one out of the egg. It >would make things much easier if I could buy sheets of the stuff, and >culture my embryos on it (laid over albumen). Please email >mlevin@fas.harvard.edu if you have any ideas. Thanks! > >Mike Levin -------------------------------------------------------------------- Dr. Richard Gordon, Department of Radiology University of Manitoba, Health Sciences Centre Rm. A246, 820 Sherbrook Street, Winnipeg, MB R3A 1R9 Canada Phone: (204) 789-3828, Fax: (204) 787-2080, Home: (204) 589-0411 E-mail: GordonR@cc.UManitoba.ca From owner-biophysics@net.bio.net Sat Oct 11 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: "Rohan H. Wickramasinghe" Newsgroups: bionet.biophysics Subject: Dielectric constants Date: 12 Oct 1997 08:47:01 +0100 Organization: Institute for Tropical Environmental Studies Lines: 38 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <61pv9l$kfp@mserv1.dl.ac.uk> Original-To: biophys@dl.ac.uk, biophys@dl.ac.uk Hello, Some time back, we presented evidence which supported the possibility of a novel regulatory mechanism for the biosynthesis of steroid hormones in the adrenal cortex. We are writing just now to ask for information as to developments in instrumentation and techniques for the estimation of dielectric constants which may have taken place in recent years and which may be of interest for possible research studies in this area. The following papers are of relevance: 1. " Regulation of corticosteroid hydroxylations: the intramitochondrial dielectric constant and proteolipids of adrenodoxin and cytochrome P450", CYTOBIOS (England) 8(1973)81-94, 2. " The regulation of corticosteroid hydroxylations. 1. An effect of inorganic ions in regulating iron-sulphur protein-dependent electron transport ", JOURNAL OF BIOENERGETICS 5(1973)151-161, and 3. " Dielectric constants and lithium ion treatment of psychiatric illness ", Abstracts, 35th Annual National Meeting of the American Federation for Clinical Research, 30 April - 1 May 1978 at San Francisco, California, CLINICAL RESEARCH 26(1978)297A. Thank you very much for any help you can give. Rohan H. Wickramasinghe, Ph.D., Institute for Tropical Environmental Studies, 41 Flower Road, Colombo 7, Sri Lanka (e-mail: rohan@ites.ac.lk) From owner-biophysics@net.bio.net Sat Oct 11 23:00:00 1997 Path: biosci!daresbury!uninett.no!news.maxwell.syr.edu!newsin.agis.net!agis!news2.dearborn.agis.net!agis!usenet From: 350@364.com Newsgroups: bionet.biophysics Subject: NLREG - Nonlinear & linear statistical regression program - 34 1012045452 KMI Date: Sun, 12 Oct 97 04:55:04 Organization: Nonlinear Regression Software Lines: 53 Message-ID: <61q6p9$qn1@news2.dearborn.agis.net> NNTP-Posting-Host: 204.181.142.21 ** Announcing NLREG -- Nonlinear Statistical Regression Program ** http://www.sandh.com/sherrod/nlreg.html NLREG is a powerful statistical analysis program for Windows 95 and NT that performs linear and nonlinear regression analysis and curve fitting. NLREG determines the values of parameters for an equation, whose form you specify, that cause the equation to best fit a set of data values. NLREG can handle linear, polynomial, exponential, logistic, periodic, and general nonlinear functions. NLREG features a full programming language with a syntax similar to C for specifying the function that is to be fitted to the data. This allows you to compute intermediate work variables, use conditionals, and even iterate in loops. With NLREG it is easy to construct piecewise functions that change form over different domains. NLREG performs true nonlinear regression, it does not transform the function into a linear form. As a result, it can handle functions that are impossible to linearize such as: Y = Amplitude*SIN(Freq*X+Phase) + A*EXP(X); Another advantage of handing the function in true nonlinear form is that the minimization of the sum of squared residual values (i.e., "least squares") is based on the true nonlinear value rather than some linearized transformation. In addition to computing the optimal values of the parameters, NLREG can generate plots of the data points and the fitted equation. In addition, it can plot the distribution of residual values. In addition to performing classic nonlinear regression, NLREG can be used to find the root or minimum value of a general nonlinear function. It can also be used in a special form where the independent variable is omitted; an interesting application of this is "circular regression" where a circle is fitted to a set of data points. NLREG is in use at hundreds of universities, laboratories, and government agencies around the world (including the U.S. Navy, Harvard, and Duke). The price of NLREG is only $65 ($70 if outside the USA), which is far below the cost of comparable commercial regression programs. And you can download a shareware demonstration version of NLREG to try out before you decide to purchase it. To learn more about NLREG and download your free shareware version, visit the web site: http://www.sandh.com/sherrod/nlreg.html -- 34: bionet.biophysics 1012045452 -- From owner-biophysics@net.bio.net Sun Oct 12 23:00:00 1997 Path: biosci!daresbury!uninett.no!news.maxwell.syr.edu!newsfeed.internetmci.com!152.163.199.19!portc03.blue.aol.com!audrey02.news.aol.com!not-for-mail From: lbrenne532@aol.com (LBrenne532) Newsgroups: bionet.biophysics Subject: For Sale: Health Physics, Radiation Safety reference materials Date: 13 Oct 1997 01:04:06 GMT Lines: 11 Message-ID: <19971013010401.VAA29455@ladder02.news.aol.com> NNTP-Posting-Host: ladder02.news.aol.com X-Admin: news@aol.com Organization: AOL http://www.aol.com For Sale: 20 boxes of Health Physics, Radiation Safety manuals, texts, etc. including over 5 years of Health Physics Journal and newsletters. CRC titles, NRC pubs, ionizing and non-ionizing radiation, etc. Too many to name here. Retired from industry after 15 years in health physics. US$750 or make offer. You pay shipping. E-mail LBrenne532@aol.com for complete list in ASCII. From owner-biophysics@net.bio.net Mon Oct 13 23:00:00 1997 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed4.bbnplanet.com!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!www.nntp.primenet.com!globalcenter0!news.primenet.com!nntp.primenet.com!news.asu.edu!general2.asu.edu!cousins From: cousins@imap3.asu.edu Newsgroups: bionet.biophysics Subject: isosbestic wavelengths Date: Mon, 13 Oct 1997 21:10:11 -0700 Organization: Arizona State University Lines: 2 Message-ID: NNTP-Posting-Host: general2.asu.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: cousins@general2.asu.edu Help me out if ya would. What causes pH dyes to emit isosbestic wavelengths when excited? Thanks. From owner-biophysics@net.bio.net Mon Oct 13 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!199.60.229.5!newsfeed.direct.ca!news.uoregon.edu!oregon.uoregon.edu!MAYNARD From: maynard@oregon.uoregon.edu (Tom Maynard) Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.molbio.methds-reagnts,sci.engr.biomed,sci.med Subject: Re: looking for slippery, permeable, artificial membrane Date: 14 Oct 1997 02:22:13 GMT Organization: University of Oregon, Eugene, Oregon Lines: 18 Message-ID: <61ul0l$edp$1@pith.uoregon.edu> References: <61ouqa$p7h$1@news.fas.harvard.edu> Reply-To: maynard@oregon.uoregon.edu NNTP-Posting-Host: oregon.uoregon.edu X-Trace: pith.uoregon.edu 876795733 14777 (None) 128.223.32.18 X-Complaints-To: usenet@news.uoregon.edu Xref: biosci bionet.biophysics:3609 bionet.cellbiol:8233 bionet.molbio.methds-reagnts:61977 In article <61ouqa$p7h$1@news.fas.harvard.edu>, mlevin@login5.fas.harvard.edu (Michael Levin) writes: > >Can someone suggest where I can buy some of the following: a membrane >(artificial) which is slippery and very smooth - so that tissues can grow >and expand on it, and is also permeable to nutrients and other molecules. >Perhaps something like this is used in organ or tissue culture, etc. Millipore makes tissue culture plate inserts, with different types of filter material and different pore sizes, that can be inserted into dishes for culturing cells. It's called "Millicell" in their catalog, and there are quite a few different types (size, pore size, filter type). Someone in our lab successfully used them for coculture experiments a while back, for what it's worth.. --Tom Maynard Lab Slave From owner-biophysics@net.bio.net Mon Oct 13 23:00:00 1997 Path: biosci!bloom-beacon.mit.edu!howland.erols.net!newsfeed.internetmci.com!152.163.199.19!portc03.blue.aol.com!audrey01.news.aol.com!not-for-mail From: huntpharm@aol.com (Huntpharm) Newsgroups: bionet.biophysics Subject: US-MD-JOB OPPORTUNITY IN CELL CULTURE Date: 14 Oct 1997 12:49:41 GMT Lines: 12 Message-ID: <19971014124900.IAA05062@ladder01.news.aol.com> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com Organization: AOL http://www.aol.com I am looking for a Research Technician to perform cell culture. This person will be working on a project in cancer and your responsibilities will be to perform cell culture (maintain stable cell lines), perform some basic molecular biology (northern, southern and western blots), as well as, some animal handling and injections. The candidate should possess a B.S. or M.S.degree. We are a leading biotech firm with research facilities in Baltimore, Maryland and can provide excellent benefits (health insurance, dental, and vision plan, paid vacation and more). A high impact, high profile position with excellent opportunity for advancement. Please contact Scott Shanes by phone at 609-584-8733 Ext. 218, fax resume and cover letter to 609-584-9575 or E-Mail to sis@dmc10.com or sis@diedremoire.com From owner-biophysics@net.bio.net Mon Oct 13 23:00:00 1997 From: huntpharm@aol.com (Huntpharm) Newsgroups: bionet.biophysics Subject: US-MD-JOB OPPORTUNITY IN QUALITY ASSURANCE Date: 14 Oct 1997 12:49:00 GMT Lines: 18 Message-ID: <19971014124900.IAA06228@ladder02.news.aol.com> NNTP-Posting-Host: ladder02.news.aol.com X-Admin: news@aol.com Organization: AOL http://www.aol.com Path: biosci!news.ic.sunysb.edu!news-pen-15.sprintlink.net!newsfeed.nysernet.net!news.nysernet.net!207.41.200.131!news-pen-1.sprintlink.net!news-east.sprintlink.net!news-dc-26.sprintlink.net!news-peer.sprintlink.net!news.sprintlink.net!Sprint!newsfeed.internetmci.com!152.163.199.19!portc03.blue.aol.com!audrey02.news.aol.com!not-for-mail I am looking for a Scientist to work in Quality Assurance. This person will develop and validate analytical methods related to NDA commitments and foreign registry requirements for its products. The candidate will be responsible for providing guidance and leadership to QC analysts in method improvement, enhancement and transfer; managing cross validation of analytical methods; preparing reports and maintaining laboratory notebooks according to cGMP's and established SOP's; and supervising QC analysts. The position requires a Ph.D. with 1-4 years experience in the pharmaceutical industry or an MS with a minimum of 6 years in the pharmaceutical industry. Candidates must have knowledge of cGMP's and strong background in all analytical techniques; chromatography experience is needed. We are a leading biotech firm with research facilities in Baltimore, Maryland and can provide excellent benefits (health insurance, dental, and vision plan, stock options, bonus program, paid vacation and more). A high impact, high profile position with excellent opportunity for advancement. Please contact Scott Shanes by phone at 609-584-8733 Ext. 218, fax CV and cover letter to 609-584-9575 or E-Mail to sis@dmc10.comsis@diedremoire.com From owner-biophysics@net.bio.net Tue Oct 14 23:00:00 1997 From: villowan@earthlink.net (Charlsie Patterson) Newsgroups: bionet.biophysics Subject: Excellent Position in Genetics Initiative Date: Wed, 15 Oct 1997 12:59:28 GMT Organization: EarthLink Network, Inc. Lines: 30 Message-ID: <622eqb$h4u@suriname.earthlink.net> NNTP-Posting-Host: 38.30.41.226 X-Newsreader: Forte Free Agent 1.0.82 Path: biosci!news.ic.sunysb.edu!news-pen-15.sprintlink.net!newsfeed.nysernet.net!news.nysernet.net!207.41.200.131!news-pen-1.sprintlink.net!news-east.sprintlink.net!news-dc-26.sprintlink.net!news-peer.sprintlink.net!news-peer-east.sprintlink.net!news.sprintlink.net!Sprint!news.idt.net!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!prodigy.com!nntp.earthlink.net!usenet This is a permanent position with a huge multinational Pharmecuetical company. Excellent pay and benefits along with unlimited growth potential. Our client company is agressivly seeking to fill this position and will be covering all fees as well as relocation for a suitable candidate. If qualified and interested, please contact: Charlsie Patterson Global Staffing and Recruiting villowan@earthlink.net or phone: (919) 871-0070 or fax credentials to: (919) 871-0030 Consultant: Genetic Initiative Summary of duties: Perform statistical analysis of data generated in support of company's Genetic Initiative. Develop and improve existing analysis software. Interact with researchers in growing Genetic Initiative to define and implement genetics strategy. Evaluate statistical aspects of new technologies. Minimum Qualifications: Ph.D. in statistics or quantitative genetics with some applied analysis experience. Genetics background is essential. Experience with UNIX file systems and SAS or other statistical analysis package required. Excellent teamwork, interpersonal and communication skills are necessary. From owner-biophysics@net.bio.net Tue Oct 14 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: <33879476@prodigy.com> Newsgroups: bionet.biophysics Subject: (guest) TONER Date: 15 Oct 1997 05:03:47 +0100 Lines: 49 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <621fb3$a6k@mserv1.dl.ac.uk> Original-To: friend@piblic.com BENCHMARK PRINT SUPPLY 1091 Redstone Lane Atlanta GA 30338 (770)399-0953 ***NEW ANNOUNCEMENT**** ********OUR NEW, LASER/FAX TONER CARTRIDGE,PRICES******** NOW AS LOW AS $39 & UP WE CARRY MOST ALL LASER PRINTER CARTRIDGES, FAX SUPPLIES AND COPIER TONERS AT WAREHOUSE PRICES INCLUDING: HEWLETT PACKARD SERIES 2,3,4,5,2P,3P,4P,5P,4L,5L,3SI,4SI,5SI IBM/LEXMARK SERIES 4019,4029,4039,4049,OPTRA EPSON SERIES 1000,1100,1500,6000,7000,8000 NEC SERIES 95 CANON FAX/LASER CARTRIDGES INCLUDING 700/770/5000/7000 CANON COPIER PC SERIES INCLUDING 3,6RE,7,10 HP FAX SERIES INCLUDING FX1,FX2,FX3 PRICES CHANGE WEEKLY, PLEASE CALL TO GET MOST RECENT PRICING AND AVAILABILITY. 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TO BE REMOVED FROM OUR LIST CLICK ON BLUE BELOW AND IN THE HEADER TYPE "REMOVE". click here From owner-biophysics@net.bio.net Tue Oct 14 23:00:00 1997 Path: biosci!daresbury!uninett.no!newsfeed.nacamar.de!news-feed.inet.tele.dk!news.misty.com!nntp.upenn.edu!axe1.med.upenn.edu!axelsen From: axelsen@axe1.med.upenn.edu (Paul H Axelsen) Newsgroups: bionet.biophysics Subject: POSTDOCTORAL POSITIONS AVAILABLE: Molecular Recognition and Membrane Biophysics Date: 15 Oct 1997 22:33:37 GMT Organization: University of Pennsylvania Lines: 45 Message-ID: <623gc1$584$11@netnews.upenn.edu> NNTP-Posting-Host: axe1.med.upenn.edu X-Newsreader: TIN [version 1.2 PL2-upenn1.1] POSTDOCTORAL POSITIONS AVAILABLE: Molecular Recognition and Membrane Biophysics Location: Department of Pharmacology University of Pennsylvania Philadelphia, Pennsylvania, USA Projects include studies of antibiotics, fusogenic peptides, and blood coagulation proteins on lipid membranes - using novel spectroscopic (infrared and UV), computational, and mass spectrometric techniques - with a view towards rational drug design. The positions are stably funded and available immediately. Requirements: Strong background in chemistry and biology (at least some background in both fields required, i.e. biochemstry or biological chemistry - physics is also very helpful) Doctoral degree (Ph.D., M.D., D.Sc., or equivalent) Minimum 2 year committment Talent for mastering sophisticated new technology Interest in working on important biomedical problems Some general information about the lab is available at http://axe2.med.upenn.edu Interest persons should inquire by email to axe@pharm.med.upenn.edu or write/call: Prof. Paul H. Axelsen Departments of Pharmacology and Medicine, Infectious Diseases Section University of Pennsylvania Rooms 130/131 John Morgan Bldg 3620 Hamilton Walk Philadelphia, PA 19104-6084 215-898-9238 / 9766 (tel) 215-573-2236 (fax) -- ------------------------------------------------------------------------------ Axe@pharm From owner-biophysics@net.bio.net Tue Oct 14 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!4.1.16.34!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!207.206.0.75!iagnet.net!198.6.0.23!uunet!in1.uu.net!news.internetsat.com!not-for-mail From: azereza Newsgroups: bionet.biophysics Subject: Could U help me? Date: Wed, 15 Oct 1997 19:23:53 +0000 Organization: NetSat Inc. Lines: 24 Message-ID: <3445183E.4EB3@hotmail.com> Reply-To: azereza@hotmail.com NNTP-Posting-Host: port2-48.nordnet.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Macintosh; I; 68K) I'm trying hard to translate this text to English, but it seems not work. Could you plz correct it for me? I have to present this by noon tomorrow. Here is the part I have problem. The linear DC power is capable of supplying the DC Voltage from 0 to +30 Volt and 0 to -30 Volt ( providing 1 ampere current at most ), and of 5 Volt Voltage supply ( providing 2 ampere current at most ). The section of the linear DC power providing the DC Voltage from 0 to +30 Volt and 0 to -30 Volt is designed without using processed IC whileas the design of the section providing 5 Volt Voltage supply includes IC regulator and short-circuit protection. The intent here is to prevent any damages that may occur with the linear DC power generator. This DC power supply also includes the over-voltage protection to protect the damage which may occur with any devices in the circuit or the damages in the circuit itself. REPLY TO: u3811771@student.chula.ac.th Thanx in advance! Maia From owner-biophysics@net.bio.net Tue Oct 14 23:00:00 1997 Path: biosci!UCONNVM.UCONN.EDU!RHODES From: RHODES@UCONNVM.UCONN.EDU Newsgroups: bionet.biophysics Subject: Abstract sponsor??? Date: 15 Oct 1997 08:41:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: <971015.114151.EDT.RHODES@UConnVM.UConn.Edu> NNTP-Posting-Host: net.bio.net OK - I don't know whether such "solicitation" is frowned upon, but I'll give it a shot - if you can help, I'd appreciate it. I have materials that students would like to see presented at the Kansas City meetings, but the students will not be able to attend (due to course conflicts and $ - in both cases). I also have an abstract that needs to be approved by a collaborator/company, and the turnaround is very slow - (read as "will not know by today but most likely will know before 24th"). Thus, I would like to be able to get >1 poster submitted but the students in question are nonmembers. The question is: <<>> Is there anyone out there who is a member and is not planning to submit a paper or sponsor one, and is willing to lend his/her name to our work? I'd appreciate it. (PS - if the society admin./exec. readers don't like the idea of "soliciting," please let me know so I can post a retraction.) | O==O | | David G. Rhodes O==O Phone 860-486-5413 | | School of Pharmacy; U-92 O==O Fax 860-486-4998 | | University of Connecticut O==O | | Storrs, CT 06269-2092 O==O rhodes@uconnvm.uconn.edu | |_____________________________O==O____________________________| CAUTION: Dates on calendar are closer than they appear! From owner-biophysics@net.bio.net Wed Oct 15 23:00:00 1997 Path: biosci!rutgers!nntp.upenn.edu!newsserver.jvnc.net!news.pn.com!nntp.pn.com!main.Germany.EU.net!main.de.uu.net!fu-berlin.de!news-peer.gsl.net!news.gsl.net!gip.net!news-peer.sprintlink.net!news.sprintlink.net!Sprint!newsfeed.internetmci.com!205.219.166.144!banzai!swen.emba.uvm.edu!elk.uvm.edu!hbeernin From: hbeernin@elk.uvm.edu (Hans Beernink) Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.molbio.methds-reagnts,sci.engr.biomed,sci.med Subject: Re: looking for slippery, permeable, artificial membrane Followup-To: bionet.biophysics,bionet.cellbiol,bionet.molbio.methds-reagnts,sci.engr.biomed,sci.med Date: 16 Oct 1997 02:53:19 GMT Organization: University of Vermont Lines: 17 Message-ID: <623viv$3pk@swen.emba.uvm.edu> References: <61ouqa$p7h$1@news.fas.harvard.edu> <61ul0l$edp$1@pith.uoregon.edu> NNTP-Posting-Host: elk.uvm.edu X-Newsreader: TIN [version 1.2 PL2] Xref: biosci bionet.biophysics:3619 bionet.cellbiol:8248 bionet.molbio.methds-reagnts:62041 Tom Maynard (maynard@oregon.uoregon.edu) wrote: : In article <61ouqa$p7h$1@news.fas.harvard.edu>, mlevin@login5.fas.harvard.edu (Michael Levin) writes: : > : >Can someone suggest where I can buy some of the following: a membrane : >(artificial) which is slippery and very smooth - so that tissues can grow : >and expand on it, and is also permeable to nutrients and other molecules. : >Perhaps something like this is used in organ or tissue culture, etc. and what about soluble collagen for TC? two options for this one- 1) a gel (approx. 0.3- 2mm thick) which mimics a dermal matrix, and 2) a thin layer (e.g. thickness of a few molecules or so) that provides a surface for attachment. Either might be suitable for your expts, although you're not very explicit about your needs. Best, Hans From owner-biophysics@net.bio.net Thu Oct 16 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.biophysics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Oct 1997 02:00:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199710170900.CAA09440@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-biophysics@net.bio.net Thu Oct 16 23:00:00 1997 Path: biosci!agate.berkeley.edu!howland.erols.net!news.maxwell.syr.edu!news-feed1.tiac.net!news-master.tiac.net!news@tiac.net From: Property Digest Newsgroups: bionet.biophysics Subject: National Biotech Register(NatBio) Date: Fri, 17 Oct 1997 08:51:39 -0700 Organization: U.S. Real Estate Register Lines: 5 Message-ID: <3447898B.183@barryinc.com> NNTP-Posting-Host: p2.ts8.lowel.ma.tiac.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Win16; I) NatBio has added a new page to our web site; http://www.barryinc.com/bio This new page allows companies in the Biotech Industries to announce new products and developments. NatBio also has a page showing job listings in the Biotech fields, as well as a calendar of events page. From owner-biophysics@net.bio.net Fri Oct 17 23:00:00 1997 Path: biosci!agate!spool.mu.edu!uwm.edu!vixen.cso.uiuc.edu!news-peer.sprintlink.net!news.sprintlink.net!Sprint!news.maxwell.syr.edu!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!prodigy.com!nntp.earthlink.net!usenet From: prismx@earthlink.net (Claire Haller) Newsgroups: bionet.neuroscience,bionet.biophysics,bionet.cellbiol Subject: SCIENCE-WEEK: This Week's Headlines (17 Oct 97) Date: Sat, 18 Oct 1997 16:24:57 GMT Organization: SCIENCE-WEEK Lines: 22 Message-ID: <62ao17$1f3@argentina.earthlink.net> Reply-To: prismx@earthlink.net NNTP-Posting-Host: 153.36.138.177 X-Newsreader: Forte Free Agent 1.0.82 Xref: biosci bionet.neuroscience:20558 bionet.biophysics:3622 bionet.cellbiol:8260 Headlines in This Week's SCIENCE-WEEK (October 17, 1997) For free Email subscription to complete news reports, send SUB SW to prismx@earthlink.net 1. A Conflict Between Seismologists and Defense Analysts 2. An Essay on the Confusion Over Cloning 3. Revelations Concerning Lisa Meitner and the Nobel Prize 4. A Postulate of Possible Sub-Surface Planetary Life Habitats 5. Squeezed Light Experiments Reveal Non-Classical Correlations 6. New Satellite Data Maps the Ocean Floor 7. Gravito-Optical Surface Trap for Super-Cooled Atoms 8. Reversible Metallic State Tuning of Quantum Dot Monolayers 9. Method for Controlled Elaboration of Macromolecular Dendrimers 10. An Unusual Idea Concerning Honeybees and Quarks The Editors SCIENCE-WEEK A Free Weekly Digest of the News of Science prismx@earthlink.net http://members.aol.com/sciweek From owner-biophysics@net.bio.net Sat Oct 18 23:00:00 1997 From: Anindya Bhattachrya Newsgroups: bionet.biophysics Subject: Noise Analysis Date: Sat, 18 Oct 1997 21:56:38 -0400 Organization: SUNY Buf. Lines: 6 Message-ID: <344968D6.1B4F@acsu.buffalo.edu> NNTP-Posting-Host: ubppp-246-042.ppp-net.buffalo.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit NNTP-Posting-User: ab4 X-Mailer: Mozilla 2.02Gold (Win95; I) Path: biosci!news.ic.sunysb.edu!news-pen-15.sprintlink.net!newsfeed.nysernet.net!news.nysernet.net!207.41.200.131!news-pen-1.sprintlink.net!news-east.sprintlink.net!news-dc-26.sprintlink.net!news-peer.sprintlink.net!news-sea-19.sprintlink.net!news-in-west.sprintlink.net!news.sprintlink.net!Sprint!128.226.1.24!bingnews.binghamton.edu!news.acsu.buffalo.edu!acsu.buffalo.edu!not-for-mail Hi , What is a good program that I can use for noise analysis on whole cell calcium currents (Mac Preferable, but PC based software suggestions also welcome). Anindya From owner-biophysics@net.bio.net Sat Oct 18 23:00:00 1997 Path: biosci!news.ohsu.edu!not-for-mail From: Matt Jones Newsgroups: bionet.biophysics Subject: Re: Noise Analysis Date: 19 Oct 1997 19:12:34 GMT Organization: Vollum Institute Lines: 16 Distribution: world Message-ID: <62dm32$f06$1@fremont.ohsu.edu> References: <344968D6.1B4F@acsu.buffalo.edu> NNTP-Posting-Host: 137.53.99.48 Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Nuntius 2.0.4_68K X-XXDate: Mon, 19 Oct 1987 20:23:23 GMT In article <344968D6.1B4F@acsu.buffalo.edu> Anindya Bhattachrya, ab4@acsu.buffalo.edu writes: >Hi , > > What is a good program that I can use for noise analysis on >whole cell calcium currents (Mac Preferable, but PC based software >suggestions also welcome). I've used AxoGraph (Axon Instruments) on the Mac for non-stationary noise analysis. It's a pretty nice all-around time-series analysis package. I imagine you can use it for finding power spectra too, but I haven't done that. Cheers, Matt From owner-biophysics@net.bio.net Sun Oct 19 23:00:00 1997 Path: biosci!agate!spool.mu.edu!uwm.edu!www.nntp.primenet.com!globalcenter0!news.primenet.com!nntp.primenet.com!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!prodigy.com!nntp.earthlink.net!usenet From: kshreder@znet.com Newsgroups: bionet.biophysics Subject: Updated WWWsite: The Antibody Resource Page Date: Sun, 19 Oct 1997 16:59:16 -0700 Organization: The Antibody Resource Page Lines: 62 Message-ID: <344A9ED4.2B02@znet.com> Reply-To: kshreder@znet.com NNTP-Posting-Host: 153.35.103.118 Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 3.0Gold (Win16; U) Updated WWWsite: The Antibody Resource Page The Antibody Resource Page has been recently updated. The page will be invaluable to researchers and educators alike. Here is just some of what can be found on the page: 1. How to Find an Antibody - a variety of ways on and off the web to find the antibody you are looking for. 2. Online Companies - links to over 110 companies that sell antibodies or antibody related products. Is your company listed on this page? 3. Antibody Image Gallery - some animated gifs have recently been added 4. Bulletin Board - Have a question or have an answer? Then stop by and post a message. 5. Educational Resources - a variety of new links have been added. There are links to pages on immunochemistry, antibody production, autoimmunity, vaccines, immunology and much more. This page is divided up into sections on research, educational, and health resources. ...and there is much more. Check it out at: http://www.antibodyresource.com/ Ps. Don’t forget to visit our sponsors, Research Diagnostics, Inc. (http://www.researchd.com/absort1.htm) and Lab Vision Corporation (http://www.labvision.com/)! Updated WWWsite: The Antibody Resource Page The Antibody Resource Page has been recently updated. The page will be invaluable to researchers and educators alike. Here is just some of what can be found on the page: 1. How to Find an Antibody - a variety of ways on and off the web to find the antibody you are looking for. 2. Online Companies - links to over 110 companies that sell antibodies or antibody related products. Is your company listed on this page? 3. Antibody Image Gallery - some animated gifs have recently been added 4. Bulletin Board - Have a question or have an answer? Then stop by and post a message. 5. Educational Resources - a variety of new links have been added. There are links to pages on immunochemistry, antibody production, autoimmunity, vaccines, immunology and much more. This page is divided up into sections on research, educational, and health resources. ...and there is much more. Check it out at: http://www.antibodyresource.com/ Ps. Don’t forget to visit our sponsors, Research Diagnostics, Inc. (http://www.researchd.com/absort1.htm) and Lab Vision Corporation (http://www.labvision.com/)! From owner-biophysics@net.bio.net Sun Oct 19 23:00:00 1997 Path: biosci!agate!howland.erols.net!newsfeed.nacamar.de!newscore.univie.ac.at!news.iif.hu!news From: Zsuzsa Sasvari Newsgroups: bionet.biophysics Subject: electron microscopy of protein Date: Mon, 20 Oct 1997 11:34:34 +0200 Organization: ABC Lines: 15 Message-ID: <344B25AA.5D09@abc.hu> NNTP-Posting-Host: che-pc09.abc.hu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.03Gold (Win95; I) Dear... We have a problem with a large- 65kD,12 nm protein. We have connected the monomers via their carbohydrate moieties.Judged by size exclusion chromatography and gel electrophoresis it seems larger than 6 monomer linked. Better resolution can not be achieved. Activity measurements showed that presumingly 8 monomer are linked. When we saw the e.micrograph, the longest white stripes were about 110 nm long. However we have seen several shorter ones as well....When we enlarged a monomer sized white blub, it seemed a wunderfull monomer. Can it be told that all the polimers are 8-9 monomer long but the shorter ones are curled ? Or can you suggest a way how the exact size can be determined? From owner-biophysics@net.bio.net Tue Oct 21 23:00:00 1997 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed4.bbnplanet.com!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-fra.maz.net!news-hh.maz.net!unlisys!news.snafu.de!cs.tu-berlin.de!zrz.TU-Berlin.DE!news-ber1.dfn.de!news-ham1.dfn.de!newsserver.rrzn.uni-hannover.de!not-for-mail From: Wolfgang Tra