From owner-biophysics@net.bio.net Sun May 03 23:00:00 1998 Path: biosci!MAILEXCITE.COM!algree From: algree@MAILEXCITE.COM Newsgroups: bionet.biophysics Subject: Free Allergy Screening! Date: 4 May 1998 16:48:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: <199805042348.QAA27980@net.bio.net> NNTP-Posting-Host: net.bio.net /////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////// You can now add allergy screening and custom immunotherapy to your practice- and there are no upfront costs. Benefits include continuity of care for the patient, excellent desensitization programming, and significantly increased revenues for your practice. Complete program includes training, laboratory based allergy screening, and complete immunotherapy program in injection ready format. See how "Allergy Treatment is Made Easy" through the BioSafe, "Is Life Making You Sick" allergy treatment program. Register for your free allergy screening kits and complete information package: http://www.freeyellow.com/members3/alrgee/ From owner-biophysics@net.bio.net Sun May 03 23:00:00 1998 Path: biosci!agate!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!EU.net!cern.ch!news From: "lakshmi" Newsgroups: bionet.biophysics Subject: Inclusion bodies Date: Mon, 4 May 1998 15:12:11 +0530 Organization: CERN - European Laboratory for Particle Physics Lines: 12 Message-ID: <6ik2dd$p2g$1@sunnews.cern.ch> NNTP-Posting-Host: tifrc4.tifr.res.in X-Trace: sunnews.cern.ch 894274797 25680 (None) 158.144.21.4 X-Complaints-To: news@sunnews.cern.ch X-Newsreader: Microsoft Outlook Express 4.71.1712.3 X-MimeOLE: Produced By Microsoft MimeOLE V4.71.1712.3 Can anyone help me as I am having problems dissolving my inclusion bodies after expression of my protein. sanjay -- *********************************************************** "Some men see things as they are and say, why; I dream things that never were and say, why not." -Geroge Bernard Shaw From owner-biophysics@net.bio.net Tue May 05 23:00:00 1998 Path: biosci!canr1.cag.uconn.edu!gberkowi From: gberkowi@canr1.cag.uconn.edu (Gerry Berkowitz) Newsgroups: bionet.biophysics Subject: Post-Doctoral Position- UCONN Date: 6 May 1998 07:08:24 -0700 Organization: Department of Plant Science, Univ. of CT Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: <35506E80.D860D9E0@canr1.cag.uconn.edu> Reply-To: gberkowi@canr1.cag.uconn.edu NNTP-Posting-Host: net.bio.net Post-doctoral research associate. A plant molecular biology lab focused on the cloning and molecular characterization of ion channels and transporters seeks a scientist trained in standard molecular techniques. The research program could involve methods related to library screening, DNA sequencing, subcloning and expression of plant cDNAs in mutant yeast and bacterial systems, plant transformation, RNA isolation, antibody production, PCR-RACE, Southern and Northern analysis, and in vitro translation. Our laboratory has recently identified a number of novel genes encoding plant ion channels. We currently have expertise related to the heterologous expression of ion channel genes in Xenopus laevis frog oocytes for electrophysiological (voltage clamp) analysis of induced ion currents. This research program provides an excellent opportunity for someone experienced in molecular biology to develop expertise in ion channel gene characterization. Contact: Dr. Gerry Berkowitz, Professor and Head, Dept. of Plant Science, U- 67, 1376 Storrs Rd., Univ. Conn., Storrs, CT 06269 Phone 860-486-5940 or 2925; FAX: 860- 486-0682; Email gberkowi@canr1.cag.uconn.edu From owner-biophysics@net.bio.net Tue May 05 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!194.72.7.126!btnet-peer!btnet!news-lond.gip.net!news.gsl.net!gip.net!nntp.news.xara.net!xara.net!server6.netnews.ja.net!server4.netnews.ja.net!aber!not-for-mail From: prm@aber.ac.uk (Pedro Mendes) Newsgroups: bionet.metabolic-reg,bionet.biophysics,bionet.cellbiol Subject: Grad student participation in GRC Conf. Macromolecular Organization Followup-To: bionet.metabolic-reg Date: 4 May 1998 15:58:09 GMT Organization: Univ Wales Aberystwyth Lines: 37 Distribution: world Message-ID: <6ikoih$69b$1@wolfskin.aber.ac.uk> NNTP-Posting-Host: pcjafc.dbs.aber.ac.uk Mime-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII X-Newsreader: WinVN 0.99.9 (Released Version) (alpha 32bit) Keywords: conference Xref: biosci bionet.metabolic-reg:2134 bionet.biophysics:4184 bionet.cellbiol:9408 I'm posting this on behalf of Douglas Kell Gordon Conference on Macromolecular Organization and Cell Function (September 13-17, 1998, Oxford University) http://gepasi.dbs.aber.ac.uk/grc98.html Conference Co-Chairs Douglas Kell and John Wilson wish to encourage graduate student participation in the Gordon Conference on Macromolecular Organization and Cell Function (September 13-17, 1998, Oxford University). We realize that costs can be a factor in limiting that participation, and have decided to adopt a policy that will provide a $250 contribution toward the conference fee for graduate student registrants, i.e., the net conference fee for graduate student participants will be $520 ($470 with early registration discount). We will consider requests for additional funding in individual cases, based on special needs. All supplements for graduate student participants will be subject to limitations of available funding, and cannot be guaranteed. Thus, to ensure favorable consideration, graduate student participants should register for the conference as soon as possible. Application can be made through the conference web site (http://gepasi.dbs.aber.ac.uk/grc98.html). Please bring this to the attention of any graduate students that you think may be interested in attending this conference. John E. Wilson Department of Biochemistry Michigan State University East Lansing, MI 48824-1319 FAX: (517) 353-9334 Phone: (517) 355-0200 E-mail: WILSONJ@PILOT.MSU.EDU ******************************************************************** *Douglas B. Kell Phone: +44 1970 622334 * *Cledwyn Building Fax: +44 1970 622354 * *Institute of Biological Sciences * *University of Wales, Email: dbk@aber.ac.uk * *Aberystwyth SY23 3DA, U.K. http://gepasi.dbs.aber.ac.uk/home.htm * ******************************************************************** From owner-biophysics@net.bio.net Tue May 05 23:00:00 1998 Path: biosci!STRETCH.MED.BUFFALO.EDU!qub From: qub@STRETCH.MED.BUFFALO.EDU (QuB) Newsgroups: bionet.biophysics Subject: "Practical Ion Channel Kinetics" Date: 5 May 1998 19:38:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <354FCCDD.EEEB218E@stretch.med.buffalo.edu> NNTP-Posting-Host: net.bio.net "Practical Ion Channel Kinetics" June 13-14 or July 18-19 School of Biomedical Sciences SUNY Buffalo This is a two-day hands-on course, taught by Drs Fenq Qin, Tony Auerbach and Fred Sachs. It is designed to teach analysis of single channel data using the programs from the QUB Maximum Likelihood Software Suite. The QUB software is free, and is available for downloading from this www.qub.buffalo.edu Further details are available from www.qub.buffalo.edu or from qub@stretch.med.buffalo.edu From owner-biophysics@net.bio.net Tue May 05 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!panix!news.panix.com!not-for-mail From: k y n n Newsgroups: bionet.biophysics Subject: ISO tiniest proteins Date: 6 May 1998 18:13:22 GMT Organization: we don't want no stinking spam Lines: 33 Message-ID: <6iq982$jch@news1.panix.com> NNTP-Posting-Host: panix3.nfs100.access.net X-No-Archive: yes Hello. I'd like to find out what are the smallest known *free-folding* proteins in the following categories: 1. all alpha (but with a tertiary structure; i.e. not helical peptides) 1.i helix bundles 2. all beta 3. no secondary structure (helix or b-sheet) By "free-folding" I mean those that can fold in vitro to their tertiary structure without the aid of metal cofactors, disulfide bonds, chaperonins, etc., and exist as monomers in solution. My interest is not limited to naturally-occurring or biologically active proteins; artificially generated proteins as well as protein fragments, as long as they fold independently, have a stable, well-defined (and published!), tertiary structure, are also OK. Does anybody know how to go about finding out what I'm looking for? (I've searched for recent papers on the matter, but the topic of size records in proteins doesn't seem terribly popular.) Thanks in advance, Regards, K. -- To reply directly by e-mail, remove the upper-case letters from the return address in the header. From owner-biophysics@net.bio.net Wed May 06 23:00:00 1998 Path: biosci!MAILEXCITE.COM!Screening From: Screening@MAILEXCITE.COM Newsgroups: bionet.biophysics Subject: Free Allergy Screening! Date: 7 May 1998 10:49:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: <199805071749.KAA24286@net.bio.net> NNTP-Posting-Host: net.bio.net ///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////// You can now add allergy screening and custom immunotherapy to your practice- and there are no upfront costs. Benefits include continuity of care for the patient, excellent desensitization programming, and significantly increased revenues for your practice. Complete program includes training, laboratory based allergy screening, and complete immunotherapy program in injection ready format. See how "Allergy Treatment is Made Easy" through the BioSafe, "Is Life Making You Sick" allergy treatment program. Register for your free allergy screening kits and complete information package: http://www.freeyellow.com/members3/alrgee/ From owner-biophysics@net.bio.net Thu May 07 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.wli.net!ais.net!usenet.INS.CWRU.Edu!po.CWRU.Edu!jjc4 From: jjc4@po.CWRU.Edu (James J. Campanella) Newsgroups: bionet.biophysics Subject: cuvette question Date: 8 May 1998 17:37:42 GMT Organization: Case Western Reserve University, Cleveland, OH (USA) Lines: 21 Message-ID: <6ivft6$4g4$1@pale-rider.INS.CWRU.Edu> Reply-To: jjc4@po.CWRU.Edu (James J. Campanella) NNTP-Posting-Host: owl.ins.cwru.edu Dear netters, I have what may be considered a silly question. We all know that in looking at UV absorbance in a spectrophotometer that we use quartz cuvettes because glass absorbs UV light and doesn't give you an accurate answer. But, can you use quartz cuvettes in looking at the white light absorbance of something in a spec? In other words, does a lab need both a set of glass AND quartz cuvettes? I have not been able to come up with a good reason why not. Any comments? Thanks, Jim Campanella Lehigh University Dept. of Biological Sciences -- **The Past tempts us, the present confuses us, and the future frightens us . . . and our lives slip away, moment by moment, lost in that vast terrible in-between** -The late Centauri Emperor From owner-biophysics@net.bio.net Thu May 07 23:00:00 1998 Path: biosci!news.ohsu.edu!not-for-mail From: Matt Jones Newsgroups: bionet.biophysics Subject: Re: cuvette question Date: 8 May 1998 17:52:40 GMT Organization: Vollum Institute Lines: 16 Distribution: world Message-ID: <6ivgp8$lcr$1@fremont.ohsu.edu> References: <6ivft6$4g4$1@pale-rider.INS.CWRU.Edu> NNTP-Posting-Host: 137.53.99.50 Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Nuntius 2.0.4_PPC X-XXDate: Fri, 8 May 1998 06:57:35 GMT In article <6ivft6$4g4$1@pale-rider.INS.CWRU.Edu> James J. Campanella, jjc4@po.CWRU.Edu writes: >But, can you use quartz >cuvettes in looking at the white light absorbance of something >in a spec? In other words, does a lab need both a set of glass >AND quartz cuvettes? I have not been able to come up with a good >reason why not. Any comments? Well, I could be wrong but it seems that if you hold a quartz cuvette up to the light and look through it, and it doesn't change the color of the light, then it ought to work for any visible light (at least within the limits of what your eyes can detect, which is probably pretty good). Cheers, Matt Jones From owner-biophysics@net.bio.net Thu May 07 23:00:00 1998 From: "S.I.Cameron" Newsgroups: bionet.biophysics Subject: Re: cuvette question Date: Fri, 08 May 1998 15:46:37 -0300 Organization: Canadian Forest Service - Atlantic Region Lines: 38 Message-ID: <3553530D.B59@fcmr.forestry.ca> References: <6ivft6$4g4$1@pale-rider.INS.CWRU.Edu> NNTP-Posting-Host: CAMES.FCMR.Forestry.CA Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (WinNT; I) Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!News.Ottawa.iSTAR.net!news.istar.net!webster.srv.gc.ca!news.NRCan.gc.ca!newsmaster@nrn2.nrcan.gc.ca James J. Campanella wrote: > > Dear netters, I have what may be considered a silly question. We > all know that in looking at UV absorbance in a spectrophotometer > that we use quartz cuvettes because glass absorbs UV light and > doesn't give you an accurate answer. But, can you use quartz > cuvettes in looking at the white light absorbance of something > in a spec? In other words, does a lab need both a set of glass > AND quartz cuvettes? I have not been able to come up with a good > reason why not. Any comments? > > Thanks, > > Jim Campanella > Lehigh University > Dept. of Biological Sciences > -- > **The Past tempts us, the present confuses us, and the future > frightens us . . . and our lives slip away, moment by moment, > lost in that vast terrible in-between** > -The late Centauri Emperor James: You are correct. Quartz can be used down to 220 or 170 nm, depending on the type. There is also IR transmissive quartz. Quartz is also less reactive than glass with some chemicals. Glass is only good to about 350 nm. For visible light work exclusively, try polystyrene cuvettes - they are cheap and disposable -- # ================================================= # | | | Dr. Stewart I. Cameron | | Canadian Forest Service - Atlantic Region | | e-mail: scameron@fcmr.forestry.ca | | | # ================================================= # From owner-biophysics@net.bio.net Thu May 07 23:00:00 1998 Path: biosci!news.Stanford.EDU!Cabal.CESspool!bofh.vszbr.cz!cpk-news-hub1.bbnplanet.com!chicago-news-feed2.bbnplanet.com!news.bbnplanet.com!news.itis.com!not-for-mail From: Petr Kuzmic Newsgroups: bionet.biophysics Subject: Re: cuvette question Date: Fri, 08 May 1998 14:00:38 -0500 Organization: BioKin Consulting Lines: 14 Message-ID: <35535656.D65FD297@biokin.com> References: <6ivft6$4g4$1@pale-rider.INS.CWRU.Edu> NNTP-Posting-Host: a3-43.itis.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.01 [en] (Win95; I) X-Priority: 3 (Normal) James J. Campanella wrote: > ...In other words, does a lab need both a set of glass > AND quartz cuvettes? The answer is "no". Quartz does not absorb any light in the visible region of the spectrum, so quart cuvettes can be used for both UV and VIS spectroscopy. --Petr _____________________________________________________________ Petr Kuzmic Ph.D. * BioKin Ltd. * Madison, WI 53708-8336, USA pkuzmic@biokin.com * http://www.biokin.com * 608.256.1269 fax From owner-biophysics@net.bio.net Thu May 07 23:00:00 1998 Path: biosci!news.Stanford.EDU!Cabal.CESspool!bofh.vszbr.cz!howland.erols.net!woodstock.news.demon.net!demon!news.demon.co.uk!demon!revealer.demon.co.uk!std From: std@revealer.demon.co.uk (Simon Dobbs) Newsgroups: bionet.biophysics Subject: Re: cuvette question Date: Fri, 8 May 1998 21:54:59 +0100 Organization: Dobbs Research Message-ID: <1d8qcsw.1v39nmuq9r63sN@revealer.demon.co.uk> References: <6ivft6$4g4$1@pale-rider.INS.CWRU.Edu> <6ivgp8$lcr$1@fremont.ohsu.edu> NNTP-Posting-Host: revealer.demon.co.uk X-NNTP-Posting-Host: revealer.demon.co.uk:158.152.19.25 X-Trace: news.demon.co.uk 894660843 nnrp-11:23731 NO-IDENT revealer.demon.co.uk:158.152.19.25 X-Complaints-To: abuse@demon.net X-Face: //I=zIlCQ@.dAHTE`{x6jLH&!2foM.)w(=vxMCC[,[_[}{Cztc=s~G%a~dZgs BBt0[9o9AljKm%g8"`>gdUQcni)nz~?ifCh^?0g X-Newsreader: MacSOUP 2.3.2 Lines: 20 Matt Jones wrote: > In article <6ivft6$4g4$1@pale-rider.INS.CWRU.Edu> James J. Campanella, > jjc4@po.CWRU.Edu writes: > >But, can you use quartz > >cuvettes in looking at the white light absorbance of something > >in a spec? In other words, does a lab need both a set of glass > >AND quartz cuvettes? I have not been able to come up with a good > >reason why not. Any comments? > > Well, I could be wrong but it seems that if you hold a quartz cuvette up > to the light and look through it, and it doesn't change the color of the > light, then it ought to work for any visible light (at least within the > limits of what your eyes can detect, which is probably pretty good). > > Cheers, > > Matt Jones or simply run a cuvette alone against air in your spec, that should be definitive, within the limits of your spec's performance. From owner-biophysics@net.bio.net Fri May 08 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsm.ibm.net!ibm.net!uunet!in3.uu.net!news.mel.aone.net.au!newsfeed-in.aone.net.au!news.mel.connect.com.au!harbinger.cc.monash.edu.au!newshost.carno.net.au!torda From: torda@rsc.anu.edu.au (Andrew Torda) Newsgroups: bionet.molec-model,bionet.info-theory,bionet.molbio.proteins,bionet.biophysics,bionet.xtallography Subject: Post-doc in Australia with Andrew Torda (biological simulation / calculation) Date: 9 May 1998 02:00:39 GMT Organization: Research School of Chemistry, Australian National University Lines: 59 Message-ID: <6j0dc7$2as$1@clarion.carno.net.au> Reply-To: Andrew.Torda@anu.edu.au NNTP-Posting-Host: 150.203.35.129 Xref: biosci bionet.molec-model:2106 bionet.info-theory:6333 bionet.molbio.proteins:12773 bionet.biophysics:4196 bionet.xtallography:4219 POST DOCTORAL POSITION in Canberra, Australia with Andrew Torda. Each year, the Research School of Chemistry at the Australian National University has a series of post-doctoral fellowships awarded on a competitive basis. Applications for the current round will be taken until about June 30, 1998. Starting dates are negotiable. There are positions in several groups. This ad mainly describes my group. The research group of Andrew Torda is oriented towards biomolecular calculation and simulation. We are working in areas such as low-resolution (protein fold recognition) force fields, refinement of experimental structures using MD simulation, mixing knowledge-based force fields and experimental data. Have a look at http://www.rsc.anu.edu.au/~torda All the projects in the group involve coding - not just applications. It would be an advantage to have a reasonable knowledge of data structures and algorithms and programming experience in a civilized language (not fortran). Currently, possible projects would be centred around some new algorithms for aligning amino acid sequences to structures, based on the force fields we have developed. We are also looking at protein fold classification using topological and graph theory methods. Projects are negotiable. Salary: $42 201 - $45 159 per annum. Grants are provided towards travel and removal. Positions are initially for two years with a possible extension to a third year. There is a housing office to help find accommodation. The Research School of Chemistry is part of the Institute of Advanced Studies which runs special research schools, in parallel to the normal teaching schools, and without any undergraduate teaching duties. The university is in the centre of Canberra (the nation's capital). Given the research orientation of the school, there is a lively academic environment. We have close contacts with the school's other theoretical groups in statistical mechanics, quantum chemistry and chemical physics. From the point of view of experimental groups, we maintain close ties to the school's NMR, X-ray crystallography and molecular biology groups. The official advertisement is at http://rsc.anu.edu.au/RSC/AcademicPositions/PDFadvert.html This contains instructions for how to apply. Although it is a good idea to establish some contact by email, the forms ultimately have to be printed out and sent here by slow mail. ------------ Andrew Torda Research School of Chemistry, ANU, ACT 0200, Australia andrew.torda@anu.edu.au http://www.rsc.anu.edu.au/~torda From owner-biophysics@net.bio.net Sat May 09 23:00:00 1998 Path: biosci!news.Stanford.EDU!Cabal.CESspool!bofh.vszbr.cz!newsfeed.uk.ibm.net!sackheads.org!news.ibm.net.il!ibm.net!news.biu.ac.il!news.huji.ac.il!not-for-mail From: "Jonathan B. Marder" Newsgroups: bionet.biophysics Subject: Re: cuvette question Date: Sun, 10 May 1998 10:24:37 +0300 Organization: Hebrew University Lines: 20 Message-ID: <6j3knt$7dn$1@news.huji.ac.il> References: <6ivft6$4g4$1@pale-rider.INS.CWRU.Edu> NNTP-Posting-Host: marder.agri.huji.ac.il Mime-Version: 1.0 Content-Type: text/plain; charset="utf-8" Content-Transfer-Encoding: 7bit X-Newsreader: Microsoft Outlook Express 4.72.2201.0 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.2201.0 James J. Campanella wrote in message <6ivft6$4g4$1@pale-rider.INS.CWRU.Edu>... > ...does a lab need both a set of glass >AND quartz cuvettes? I have not been able to come up with a good >reason why not. Any comments? It just a question of economics. Any lab. doing a moderate amount of spectroscopy can expect the occasional broken cuvette!!!!!! I generally wouldn't want to routinely risk quartz cuvettes where glass would do. Jonathan B. Marder Department of Agricultural Botany, The Hebrew University of Jerusalem Faculty of Agriculture, P.O.Box 12, Rehovot 76100, ISRAEL Phone: +972 8 9481918 Fax: +972 8 9467763 Web page: http://www.agri.huji.ac.il/~marder From owner-biophysics@net.bio.net Sun May 10 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!209.150.97.11!feeder.qis.net!WCG!news-hub.interserv.net!news.interserv.com!not-for-mail From: "Jack" Newsgroups: bionet.biophysics Subject: HTML -out?? XML in?? XML and CISCO Router Booklist Date: Sun, 10 May 1998 22:38:15 -0500 Organization: Interserv News Service Lines: 22 Message-ID: <6j5roo$ihi$1@lal.interserv.com> NNTP-Posting-Host: hd54-148.hil.compuserve.com X-Newsreader: Microsoft Outlook Express 4.71.1712.3 X-MimeOLE: Produced By Microsoft MimeOLE V4.71.1712.3 New booklists: Discover what the cutting edge technology companies are up to. Extended Markup Language (XML), a new markup language technology derived from SGML, is being adopted by many leading edge successful companies and research facilities as the new standard. XML is intended to replace HTML and provide a more robust, extensible architecture. Among its features, SGML scripting without SGML headaches. Get familiar with Dynamic HTML, Cascading Style Sheets, Document Type Definitions (DTDs), and more check http://www.a-ten.com/books/xml/ ---------- Are terms such as: CISCO, router, IPv6, protocols, ICMPv6, RIPv6, IDRPv6, BGP4+, XML, SGML, proxy, Server, Operation, TCP/IP, and IPX familiar? If not check http://www.a-ten.com/books/cisco/ Recommended Books on CISCO Routing / Networks and more --------------- Time for a break? check http://www.a-ten.com/books/dc/ Recommended Books by Douglas Coupland, author of " Microserfs" [A must read for the computer literrati] From owner-biophysics@net.bio.net Mon May 11 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.concentric.net!newsfeed1.earthlink.net!nntp.earthlink.net!usenet From: John Philo <"jphilo*NO SPAM12*"@earthlink.net> Newsgroups: bionet.biophysics,bionet.molbio.proteins Subject: Re: E-mail address for Micheal Doyle? Date: Mon, 11 May 1998 17:05:57 -0700 Organization: Alliance Protein Laboratories Lines: 19 Message-ID: <35579265.52F9@earthlink.net> References: <35574167.ED4CF774@microbio.uab.edu> Reply-To: jphilo*NO, SPAM12*@earthlink.net NNTP-Posting-Host: 209.179.14.9 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.03 (Win95; I) To: Matthew Parker Xref: biosci bionet.biophysics:4201 bionet.molbio.proteins:12783 Matthew Parker wrote: > > Hi! Can anyone give me Michael Doyle's e-mail address? (He's at > Smith-Kline Beecham). > > Thanks! > > Matthew Parker I don't have his actual address, but based on the pattern of e-mail addresses there he should be michael_l_doyle@spbphrd.com If that doesn't work, ask Preston Hensley there, c_preston_hensley@sbphrd.com -- John Philo, Alliance Protein Laboratories *** Remove "*NO SPAM12*" from return address before replying. *** From owner-biophysics@net.bio.net Tue May 12 23:00:00 1998 Path: biosci!news.Stanford.EDU!Cabal.CESspool!bofh.vszbr.cz!fu-berlin.de!newscore.univie.ac.at!193.171.255.24.MISMATCH!newsfeed03.univie.ac.at!03-newsfeed.univie.ac.at!fstgal00.tu-graz.ac.at!not-for-mail From: giessauf Newsgroups: bionet.biophysics Subject: Re: cuvette question Date: Wed, 13 May 1998 18:05:20 +0200 Organization: bbb Lines: 42 Message-ID: <3559C4BF.68C6@glvt.tu-graz.ac.at> References: <6ivft6$4g4$1@pale-rider.INS.CWRU.Edu> Reply-To: bbb NNTP-Posting-Host: fthvtpc11.tu-graz.ac.at Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 3.0 (Win95; I) James J. Campanella wrote: > > Dear netters, I have what may be considered a silly question. We > all know that in looking at UV absorbance in a spectrophotometer > that we use quartz cuvettes because glass absorbs UV light and > doesn't give you an accurate answer. But, can you use quartz > cuvettes in looking at the white light absorbance of something > in a spec? In other words, does a lab need both a set of glass > AND quartz cuvettes? I have not been able to come up with a good > reason why not. Any comments? > > Thanks, > > Jim Campanella > Lehigh University > Dept. of Biological Sciences > -- > **The Past tempts us, the present confuses us, and the future > frightens us . . . and our lives slip away, moment by moment, > lost in that vast terrible in-between** > -The late Centauri Emperor You can use quartz cuvettes also for absorbance measurement with visible light. But remember always that they are not cheap - if you break one you loss more than if you buy a set of glass cuvettes. There is also the possibility that you buy cheap styrol (plastic) or polyacryl glass which can be used only for one time. Quarz cuvettes you have always to clean and dry - in case of a great number of samples one way cuvettes have many advances. Andreas Gießauf, PhD Technical University Graz, AUSTRIA GIESSAUF@GLVT.TU-GRAZ.AC.AT From owner-biophysics@net.bio.net Tue May 12 23:00:00 1998 From: Hans du Buf Newsgroups: bionet.biophysics Subject: POSTDOC position available Date: Wed, 13 May 1998 18:23:23 -0700 Organization: Servico de News da Universidade do Algarve Lines: 18 Message-ID: <355A478B.167E@ualg.pt> NNTP-Posting-Host: pixel.uceh.ualg.pt Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0S (X11; I; IRIX 6.2 IP22) Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.wli.net!newspeer.monmouth.com!btnet-peer!btnet!news-lond.gip.net!news.gsl.net!gip.net!newsfeed.uk.ibm.net!sackheads.org!ibm.net!news-ge.switch.ch!rccn.net!news.rccn.net!ualg.pt!not-for-mail I am looking for one postdoc interested in the modeling of visual perception (spatial brightness) and doing psychophysical experiments. New project for three years. See the postscript file in the lab's homepage or email me for getting the information. Good conditions, and beach around the corner :-) ======================================================================= Prof.dr.ir. J.M.H. du Buf mailto://dubuf@ualg.pt Dept. of Electronics and Computer Science - UCEH, University of Algarve, tel (+351 89) 800900 ext 7761 Campus de Gambelas, 8000 Faro, Portugal fax (+351 89) 818560 ======================================================================= Hein's Law: Problems worthy of attack prove their worth by hitting back ======================================================================= Vision Laboratory: http://w3.ualg.pt/~dubuf/vision.html see my page ======================================================================= From owner-biophysics@net.bio.net Wed May 13 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.wli.net!peerfeed.ncal.verio.net!nntp.ni.net!news.service.uci.edu!usenet From: dmandelm@uci.edu (David Mandelman) Newsgroups: bionet.biophysics Subject: DSC model: N2 to 2U Date: 13 May 1998 06:47:11 GMT Organization: UCI Lines: 12 Message-ID: <6jbflf$o5q@news.service.uci.edu> NNTP-Posting-Host: dialin9072.slip.uci.edu Mime-Version: 1.0 Content-Type: Text/Plain; charset=ISO-8859-1 X-Newsreader: WinVN 0.99.5 I am interested in finding a mathematical model that can best describe my raw DSC data via nonlinear regression analysis. Preferably, I want a model that can accomodate a dimeric protein undergoing a transition to unfolded monomers. Otherwise, I would appreciate any advice on where I might find more info on DSC data analysis. Thank you in advance, David Mandelman dmandelm@uci.edu From owner-biophysics@net.bio.net Wed May 13 23:00:00 1998 Path: biosci!news.Stanford.EDU!Cabal.CESspool!bofh.vszbr.cz!howland.erols.net!newsfeed.internetmci.com!207.217.77.43!newsfeed1.earthlink.net!nntp.earthlink.net!not-for-mail From: John Philo <"jphilo*NO SPAM12*"@earthlink.net> Newsgroups: bionet.biophysics Subject: Re: DSC model: N2 to 2U Date: Thu, 14 May 1998 14:20:42 -0700 Organization: Alliance Protein Laboratories Lines: 30 Message-ID: <355B6029.DE2CF912@earthlink.net> References: <6jbflf$o5q@news.service.uci.edu> NNTP-Posting-Host: 1cust68.tnt2.thousand-oaks.ca.da.uu.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.05 [en] (Win95; U) To: David Mandelman David Mandelman wrote: > I am interested in finding a mathematical model that can best describe my raw > DSC data via nonlinear regression analysis. Preferably, I want a model that > can accomodate a dimeric protein undergoing a transition to unfolded monomers. > Otherwise, I would appreciate any advice on where I might find more info on > DSC data analysis. > > Thank you in advance, > > David Mandelman > > dmandelm@uci.edu David, I imagine this type of system has been analyzed by DSC before. A good place to start might be to talk to the folks at the Biocalorimetry Center at Johns Hopkins, especially because they may even have the software to handle that specific situation. I know they have a web site, but I don't have the URL handy. The person who runs the Center is David Remeta [dpremeta@biocal2.bio.jhu.edu] or you could contact Ernesto Freire. Good luck. John Philo, Alliance Protein Laboratories *** Remove "*NO SPAM12*" from return address before replying. *** From owner-biophysics@net.bio.net Wed May 13 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.gip.net!news.gsl.net!gip.net!newsfeed.nacamar.de!nntp.news.xara.net!xara.net!server5.netnews.ja.net!news.shef.ac.uk!not-for-mail From: ACSE Admissions Newsgroups: uk.sci.misc,bionet.biophysics Subject: UK students only: PhD scholarship in Adaptive Predictive Control in Biomedicine Date: Thu, 14 May 1998 16:13:11 +0100 Organization: Automatic Control & Systems Engineering, University of Sheffield, UK Lines: 43 Message-ID: <355B0A07.5ACF@acad.uk> NNTP-Posting-Host: pc054007.shef.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 2.02 (Win16; I) Adaptive Predictive Control in Biomedicine Dept of Automatic Control and Systems Engineering University of Sheffield Applications are invited for the above 3-year studentship, leading to PhD, to work in the area of Intelligent Control Systems (Soft Computing, including Fuzzy logic, Neural Networks, and Genetic Algorithms) and Model-Based Adaptive Predictive Control applied to biomedicine. The successful candidate will be involved in the software and hardware aspects of an exciting research project which will offer adequate resources, technical support, and practical experience. He/She will also have the opportunity to join a top-rated Engineering Department which achieved a Grade 5A in the last Research Assessment Exercise. Award Value: The scholarship will pay tuition fees plus a maintenance allowance to the successful student of £5,400 per year. Eligibility: You must be a UK resident and have, or expect to achieve, at least a BSc (Hons) class 2:1 or equivalent in a relevant science, maths or engineering subject. Start Date: The scholarship can commence on a date between May and October 1998 by mutual agreement. Four additional UK Research Studentships to start on or after 1 October are available which can be allocated to any research topic in the Department. Please ask for more details. For more information, please send an email,including details of your postal address to: pgacse-g@sheffield.ac.uk *************************************************** What to find out more about research topics available? Then come to our Dept Research Open Day on Tues 9 June 1pm - 4pm. Please email above for further details *************************************************** From owner-biophysics@net.bio.net Wed May 13 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!dallas-news-feed2.bbnplanet.com!news.bbnplanet.com!newsfeed.gte.net!news.maxwell.syr.edu!newsspool.doit.wisc.edu!news.itis.com!not-for-mail From: Petr Kuzmic Newsgroups: bionet.biophysics Subject: Re: DSC model: N2 to 2U Date: Thu, 14 May 1998 06:42:12 -0500 Organization: BioKin Consulting Lines: 34 Message-ID: <355AD894.92C8B476@biokin.com> References: <6jbflf$o5q@news.service.uci.edu> NNTP-Posting-Host: a9-39.itis.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.01 [en] (Win95; I) X-Priority: 3 (Normal) David Mandelman wrote: > [...] I would appreciate any advice on where I might find more info on > DSC data analysis. Try Science Citation Index, on-line in many university libraries. Medline (http://www.ncbi.nlm.nih.gov/PubMed/) might also help. This paper deals with N4 <==> 4U, so the derivation of your simpler model N2 <==> 2U should be straighforward: Biochemistry 1995 Apr 25;34(16):5309-5316 Thermodynamic analysis of the structural stability of the tetrameric oligomerization domain of p53 tumor suppressor. Johnson CR, Morin PE, Arrowsmith CH, Freire E If this weren't DSC but some other kinetic method, I'd recommend the program DYNAFIT (http://www.biokin.com/dynafit/). There you simply write something like [mechanism] D <==> D' : k1 k2 D' <==> M + M : k3 k4 M <==> M' : k5 k6 and the machine derives the mathematical model for the reaction time-course for you. -- Petr _____________________________________________________________ Petr Kuzmic Ph.D. * BioKin Ltd. * Madison, WI 53708-8336, USA pkuzmic@biokin.com * http://www.biokin.com * 608.256.1269 fax From owner-biophysics@net.bio.net Sat May 16 23:00:00 1998 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.biophysics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 May 1998 02:00:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199805170900.CAA23036@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-biophysics@net.bio.net Mon May 18 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.gip.net!news-lond.gip.net!news.gsl.net!gip.net!nntp.news.xara.net!xara.net!server5.netnews.ja.net!news.shef.ac.uk!not-for-mail From: ACSE Admissions Newsgroups: bionet.biophysics,uk.sci.misc Subject: [UK only]: PhD scholarships available Date: Tue, 19 May 1998 14:54:30 +0100 Organization: Automatic Control & Systems Engineering, University of Sheffield, UK Lines: 44 Message-ID: <35618F16.5AFB@acad.uk> NNTP-Posting-Host: pc054011.shef.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 2.02 (Win16; I) Adaptive Predictive Control in Biomedicine (other topics also available) Dept of Automatic Control and Systems Engineering University of Sheffield Applications are invited for the above 3-year studentship, leading to PhD, to work in the area of Intelligent Control Systems (Soft Computing, including Fuzzy logic, Neural Networks, and Genetic Algorithms) and Model-Based Adaptive Predictive Control applied to biomedicine. The successful candidate will be involved in the software and hardware aspects of an exciting research project which will offer adequate resources, technical support, and practical experience. He/She will also have the opportunity to join a top-rated Engineering Department which achieved a Grade 5A in the last Research Assessment Exercise. Award Value: The scholarship will pay tuition fees plus a maintenance allowance to the successful student of £5,400 per year. Eligibility: You must be a UK resident and have, or expect to achieve, at least a BSc (Hons) class 2:1 or equivalent in a relevant science, maths or engineering subject. Start Date: The scholarship can commence on a date between May and October 1998 by mutual agreement. Four additional UK Research Studentships to start on or after 1 October are available which can be allocated to any research topic in the Department. Please ask for more details. For more information, please send an email,including details of your postal address to: pgacse-g@sheffield.ac.uk *************************************************** What to find out more about research topics available? Then come to our Dept Research Open Day on Tues 9 June 1pm - 4pm. Please email above for further details *************************************************** From owner-biophysics@net.bio.net Tue May 19 23:00:00 1998 Path: biosci!agate!newsfeed.kornet.nm.kr!news.maxwell.syr.edu!netnews.com!howland.erols.net!surfnet.nl!highway.leidenuniv.nl!not-for-mail From: Marcellus Ubbink Newsgroups: bionet.biophysics Subject: position available in biological NMR Date: Wed, 20 May 1998 13:26:43 +0200 Organization: University of Leiden Lines: 51 Message-ID: <3562BDF3.2781@chem.leidenuniv.nl> NNTP-Posting-Host: chemae5.leidenuniv.nl Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01SGoldC-SGI (X11; I; IRIX 6.3 IP32) POSITION AVAILABLE: NMR STRUCTURE DETERMINATION OF CATALYTIC HAEM PROTEINS As part of the European Union TMR Research Network 'Haemworks', a grant is available at the Leiden Institute of Chemistry, Leiden University, the Netherlands, for a postdoctoral researcher or graduate student The Network involves the Universities of Leiden, Florence, Lisbon, Seville, and Southampton, and the researcher will participate in the part of the project which is aimed at the creation of small, stable enzymes with peroxidase or monooxygenase activity. This is achieved by protein engineering of a bacterial haem protein, cytochrome c-550. For a proper understanding of the factors that influence the catalytic properties of these new proteins, detailed structures of the proteins and, in particular, the active sites, are essential. The dynamics of the protein variants also need to be established in depth. The researcher will focus on the elucidation of these topics by NMR. Candidates have to be a citizen of a member state (or associated state) of the European Union. Experience with NMR on biomolecules and/or with protein biochemistry is an advantage. The grant is for one year with the possibility of an extension. Monthly pay in the DFL 2 500 - 3 500 range depending on qualifications and experience. The position is available as per May 1st, 1998. Further information can be obtained from Dr M. Ubbink (until 29 May) tel -31 71 527 4628 e-mail: m.ubbink@chem.leidenuniv.nl or Prof. Dr G. W. Canters (after 29 May) tel -31 71 527 4256 e-mail: canters@chem.leidenuniv.nl and from http://www.cordis.lu/tmr/src/lifvacls.htm Applications together with CV, list of publications and names of 2 professional referees to be sent within 3 weeks to: Prof. Dr G.W. Canters Leiden Institute of Chemistry PO Box 9502 2300 RA Leiden, The Netherlands. From owner-biophysics@net.bio.net Tue May 19 23:00:00 1998 Path: biosci!theboll.com!bol From: bol@theboll.com Newsgroups: bionet.biophysics Subject: Searching_for_Fixatives Date: 20 May 1998 15:08:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 46 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net --divider Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: 7bit Att. Sales / Export Department Re: Request for FIXATIVES A "Request for supply" for FIXATIVES which, to the best of our knowledge are being offered by you, was placed with us by one of our clients. We are a world wide sourcing firm and we are paid by our clients to find them suitable suppliers. To you, our service is totally FREE OF CHARGE !!! The information we will get from you will not only be immediately sent to this particular client but also to other clients looking for same or similar items. Since the whole operation is activated by our computers and unique software YOU HAVE TO REGISTER THE PRODUCTS ,MATERIALS, EQUIPMENT OR SERVICES you offer. Please REGISTER using our Internet interface at: http://www.thebol.com Once registered,the system will forward to you automatically the relevant to you requests. At http://www.thebol.com you can also get more information about us and our FREE for SUPPLIERS SERVICE. We are looking forward to serve you to the best of our ability. Best Regards BOL sourcing international LTD Purchasing department mailto:purchasing@thebol.com --divider-- From owner-biophysics@net.bio.net Sun May 24 23:00:00 1998 Path: biosci!newshost.lanl.gov!awabi.library.ucla.edu!208.134.241.18!newsfeed.internetmci.com!206.229.87.25!news-peer.sprintlink.net!news-backup-east.sprintlink.net!news.sprintlink.net!204.251.80.3!mercury.galstar.com!news.okstate.edu!lse405.cas.okstate.edu!user From: burnap@biochem.okstate.edu (Robert L. Burnap) Newsgroups: bionet.biophysics Subject: postdoctoral position Date: 22 May 1998 14:23:16 GMT Organization: Oklahoma State University Lines: 28 Message-ID: NNTP-Posting-Host: lse405.cas.okstate.edu POSTDOCTORAL RESEARCH ASSOCIATE POSITION Molecular Analysis of Photosynthetic Complexes An NSF-supported postdoctoral position is available immediately to study basic protein structure-function relationships of the photosystem II reaction center complex using the experimental model Synechocystis 6803. The work combines state-of-the-art molecular genetic, biochemical, and biophysical approaches to clarify the activity and light-dependent assembly of the catalytic tetramer of manganese atoms that forms the core of the H2O-splitting enzyme. A Ph.D. and a strong background in protein biochemistry and/or molecular biology is required. Preferences will be given to individuals with proven records of quality publications and to those with potentials to obtain independent funding. Please send CV along with the names, addresses, and telephone numbers of three referees to: Dr. Robert L. Burnap, Microbiology & Molecular Genetics Life Sciences East Oklahoma State University, Stillwater, Oklahoma 74078 e-mail: burnap@biochem.okstate.edu; http://www.cas.okstate.edu/micro/faculty/BURNAP/index.html. Equal opportunity employer/AA From owner-biophysics@net.bio.net Sun May 24 23:00:00 1998 Path: biosci!newshost.lanl.gov!mail2.es.net!lll-winken.llnl.gov!uwm.edu!chicago-news-feed2.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!ix.netcom.com!news.campbellhall.org!not-for-mail From: pornzone@hotmail.com Newsgroups: bionet.biophysics Subject: yoh Date: 23 May 1998 03:36:42 GMT Organization: Boo Lines: 1 Message-ID: <6k5g8a$sel$6@websites.campbellhall.org> NNTP-Posting-Host: 24.230.31.148 X-Newsreader: 2.0.12 test.. delete this message From owner-biophysics@net.bio.net Sun May 24 23:00:00 1998 Path: biosci!news.Stanford.EDU!newsfeed.concentric.net!netnews.com!howland.erols.net!surfnet.nl!highway.leidenuniv.nl!not-for-mail From: Marcellus Ubbink Newsgroups: bionet.biophysics Subject: position available in biological NMR Date: Mon, 25 May 1998 10:39:14 +0200 Organization: University of Leiden Lines: 51 Message-ID: <35692E32.2781@chem.leidenuniv.nl> NNTP-Posting-Host: chemae5.leidenuniv.nl Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01SGoldC-SGI (X11; I; IRIX 6.3 IP32) POSITION AVAILABLE: NMR STRUCTURE DETERMINATION OF CATALYTIC HAEM PROTEINS As part of the European Union TMR Research Network 'Haemworks', a grant is available at the Leiden Institute of Chemistry, Leiden University, the Netherlands, for a postdoctoral researcher or graduate student The Network involves the Universities of Leiden, Florence, Lisbon, Seville, and Southampton, and the researcher will participate in the part of the project which is aimed at the creation of small, stable enzymes with peroxidase or monooxygenase activity. This is achieved by protein engineering of a bacterial haem protein, cytochrome c-550. For a proper understanding of the factors that influence the catalytic properties of these new proteins, detailed structures of the proteins and, in particular, the active sites, are essential. The dynamics of the protein variants also need to be established in depth. The researcher will focus on the elucidation of these topics by NMR. Candidates have to be a citizen of a member state (or associated state) of the European Union. Experience with NMR on biomolecules and/or with protein biochemistry is an advantage. The grant is for one year with the possibility of an extension. Monthly pay in the DFL 2 500 - 3 500 range depending on qualifications and experience. The position is available as per May 1st, 1998. Further information can be obtained from Dr M. Ubbink (until 29 May) tel -31 71 527 4628 e-mail: m.ubbink@chem.leidenuniv.nl or Prof. Dr G. W. Canters (after 29 May) tel -31 71 527 4256 e-mail: canters@chem.leidenuniv.nl and from http://www.cordis.lu/tmr/src/lifvacls.htm Applications together with CV, list of publications and names of 2 professional referees to be sent within 3 weeks to: Prof. Dr G.W. Canters Leiden Institute of Chemistry PO Box 9502 2300 RA Leiden, The Netherlands. From owner-biophysics@net.bio.net Mon May 25 23:00:00 1998 Newsgroups: bionet.biophysics Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!128.174.5.49!vixen.cso.uiuc.edu!uchinews!midway.uchicago.edu!aekentsi From: aekentsi@midway.uchicago.edu (alex kentsis) Subject: ES-MS on small proteins X-Nntp-Posting-Host: howard-nfs.uchicago.edu Message-ID: Sender: news@midway.uchicago.edu (News Administrator) Organization: The University of Chicago X-Newsreader: TIN [version 1.2 PL2] Date: Tue, 26 May 1998 13:22:18 GMT Lines: 6 i am trying to use an electrospray-mass spectrometer with 9-14 kD proteins, but am getting severe attenuation at the detector. can someone recommend a buffer system to increase the signal:noise? From owner-biophysics@net.bio.net Mon May 25 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!la-news-feed1.bbnplanet.com!news.bbnplanet.com!newsfeed1.earthlink.net!nntp.earthlink.net!not-for-mail From: John Philo <"jphilo*NO SPAM12*"@earthlink.net> Newsgroups: bionet.biophysics Subject: Re: ES-MS on small proteins Date: Tue, 26 May 1998 13:57:27 -0700 Organization: Alliance Protein Laboratories Lines: 16 Message-ID: <356B2CB7.A7591CE7@earthlink.net> References: NNTP-Posting-Host: 1cust225.tnt1.thousand-oaks.ca.da.uu.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.05 [en] (Win95; U) To: alex kentsis alex kentsis wrote: > i am trying to use an electrospray-mass spectrometer with 9-14 kD > proteins, but am getting severe attenuation at the detector. > > can someone recommend a buffer system to increase the signal:noise? May I suggest that you post your query on the Association for Biotechnology Resource Facilities list server (you can find out how from www.abrf.org)? There are many mass spec experts in that group. John Philo, Alliance Protein Laboratories *** Remove "*NO SPAM12*" from return address before replying. *** From owner-biophysics@net.bio.net Mon May 25 23:00:00 1998 Path: biosci!CHEM.UFL.EDU!tan From: tan@CHEM.UFL.EDU (Weihong Tan) Newsgroups: bionet.biophysics Subject: Research Associate positions at University of Florid Date: 26 May 1998 07:39:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 47 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Two research associate positions at the Department of Chemistry and UF Brain Institute, University of Florida Bioanalytical Chemistry and Biophysics Applications are invited for two postdoctoral positions at the Department of Chemistry and at the UF Brain Institute, University of Florida. The successful applicant should have a strong background in ANY ONE of the following areas: Biochemical sensors; Optical microscopy and spectroscopy; Scanning probe microscopy; Bioanalytical chemistry; Biophysics; Neuroscience. We are carrying out exciting research work in bioanalytical chemistry and biophysics. Specifically, we have the following current projects: 1) novel optical microscopy for imaging and sensing; 2) scanning probe microscopy application in living cells; 3) single biomolecule detection and manipulation; 4) biochemical sensors and in-vivo monitoring. We are also studying some important biomedical problems: i) molecular and cellular mechanisms of stroke; ii) ion channel distribution and manipulation on cell membrane; iii) DNA sensors and DNA hybridization. These research activities are funded by the US National Science Foundation, the Office of Naval Research, Department of Defense and The Arnold Beckman Foundation and by industrial companies. Our group has labs both in the Chemistry Department and in the newly established UF Brain Institute. This Institute is a national center for neuroscience and brain research. At the University of Florida we have excellent working and living environment. Gainesville is only two hour drive from major cities like Orlando, Jacksonville and Tampa. The weather is excellent, and there are many outdoor activities and cultural events throughout the year. Interested applicants should make an initial contact with Prof. Tan through e-mail, or send a CV to the following address: Prof. Weihong Tan Department of Chemistry and The UF Brain Institute University of Florida, Gainesville, FL 32611-7200 e-mail: tan@chem.ufl.edu; Tel: 352-846-2410; Fax: 352-392-4651 Web page: http://www.chem.ufl.edu/Groups/Tan/ From owner-biophysics@net.bio.net Tue May 26 23:00:00 1998 Path: biosci!fcs280s.ncifcrf.gov!wtn-news-feed2.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.sprintlink.net!news.sprintlink.net!newsfeed.nacamar.de!blackbush.xlink.net!rz.uni-karlsruhe.de!news.fzk.de!usenet From: Bernhard Klueh Newsgroups: bionet.biophysics Subject: Albumin tracer and capillary leak Date: Wed, 27 May 1998 15:00:21 +0200 Organization: FZK/HZY Lines: 41 Distribution: world Message-ID: <356C0E65.B4EB264@hzy.fzk.de> Reply-To: bklueh@hzy.fzk.de NNTP-Posting-Host: hzypcc.fzk.de Mime-Version: 1.0 Content-Type: multipart/mixed; boundary="------------17A6EE95C00877A151769A05" X-Mailer: Mozilla 4.05 [de] (Win95; I) Dies ist eine mehrteilige Nachricht im MIME-Format. --------------17A6EE95C00877A151769A05 Content-Type: text/html; charset=us-ascii Content-Transfer-Encoding: 7bit Hello,

is there anybody who knows the possibilitys and propertys of the existing albumin tracer, e.g. 51Cr ?

What is the relation between albumin and capillary leaks?
 
 
 
  --------------17A6EE95C00877A151769A05 Content-Type: text/x-vcard; charset=us-ascii; name="vcard.vcf" Content-Transfer-Encoding: 7bit Content-Description: Visitenkarte für Bernhard Klueh Content-Disposition: attachment; filename="vcard.vcf" begin: vcard fn: Bernhard Klueh n: Klueh;Bernhard org: Forschungszentrum Karlsruhe HZY adr: Nancystrasse 4 F205;;;Karlsruhe;Baden Wuerttemberg;76187;Germany email;internet: bklueh@hzy.fzk.de tel;work: 07247-82 3875 tel;home: 0721-71885 note;quoted-printable:Remove "mv4" in E-mail adress=0D=0A= ICQ Number: 6123795 x-mozilla-cpt: ;0 x-mozilla-html: FALSE version: 2.1 end: vcard --------------17A6EE95C00877A151769A05-- From owner-biophysics@net.bio.net Wed May 27 23:00:00 1998 Path: biosci!rutgers!rockyd.rockefeller.edu!news-nysernet-5.sprintlink.net!news-dc-2.sprintlink.net!news-east.sprintlink.net!news-peer.sprintlink.net!news.sprintlink.net!newsxfer3.itd.umich.edu!rill.news.pipex.net!pipex!server1.netnews.ja.net!server5.netnews.ja.net!news.shef.ac.uk!not-for-mail From: Control & Systems Recruitment Newsgroups: bionet.biophysics Subject: [UK applicants only]: PhD SCholarships available Date: Thu, 28 May 1998 12:21:13 +0100 Organization: Automatic Control & Systems Engineering, University of Sheffield, UK Lines: 77 Message-ID: <356D48A9.40E8@acad.ac.uk> NNTP-Posting-Host: pc054005.shef.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 2.02 (Win16; I) UK Students only: Research Scholarships Available at the Dept of Automatic Control and Systems Engineering The University of Sheffield 1. Adaptive Predictive Control in Biomedicine Applications are invited for the above 3-year studentship, leading to a PhD, to work in the area of Intelligent Control Systems (Soft Computing, including Fuzzy logic, Neural Networks, and Genetic Algorithms) and Model-Based Adaptive Predictive Control applied to biomedicine. The successful candidate will be involved in the software and hardware aspects of an exciting research project which will offer adequate resources, technical support, and practical experience. He/She will also have the opportunity to join a top-rated Engineering Department which achieved a Grade 5 in the last Research Assessment Exercise. This studentship can commence on a date between June and October 1998 by mutual agreement. 2. Institute for Microstructural and Mechanical Processing Engineering: The University of Sheffield (IMMPETUS) Applications are invited for a 3-year PhD studentship linked with IMMPETUS. The project will involve modelling and control for improved microstructural properties of hot-worked metals, especially steel and aluminium. This encompasses nonlinear physically-based models coupled to intelligent systems techniques for control of rolling processes. This studentship commences on 1 October 1998 or later by agreement. Award Value: Each studentship will pay tuition fees plus a maintenance allowance to the successful student of £5,400 per year. Eligibility: You must be a UK resident and have, or expect to achieve, at least a BSc (Hons) class 2:1 or equivalent in a relevant science, maths or engineering subject. In addition: Three UK Research Studentships - which can be allocated to any research topic in the Department - are also available to start on or after 1 October. A minimum of a 2:1 degree is required. Please ask for more details. For more information, please contact: Graduate Admissions, Department of Automatic Control and Systems Engineering, The University of Sheffield, Mappin Street, Sheffield, S1 3JD Telephone: (0114) 222 5248 Email: pgacse-g@sheffield.ac.uk Please remember to include your postal address to ensure a quick reply. ************************** Want to know more about our dept and research interests? Then Visit our Research/MSc Open Day on Tuesday 9 June 1998 1pm - 4pm Please contact the Dept for more information ************************** From owner-biophysics@net.bio.net Sat May 30 23:00:00 1998 From: "Andrey Pugatsov" Subject: test Newsgroups: bionet.biophysics Organization: Agency "Contact" Message-ID: <01bd8c72$6f6e4560$440107d4@Ppuandr.infonet.ee> X-Newsreader: Microsoft Internet News 4.70.1155 MIME-Version: 1.0 Content-Type: text/plain; charset=KOI8-R Content-Transfer-Encoding: quoted-printable NNTP-Posting-Host: 212.7.1.68 Date: 31 May 1998 11:59:39 +0300 X-Trace: 31 May 1998 11:59:39 +0300, 212.7.1.68 Lines: 2 Path: biosci!bloom-beacon.mit.edu!news.kodak.com!news-nysernet-5.sprintlink.net!news-dc-2.sprintlink.net!news-east.sprintlink.net!news-peer.sprintlink.net!news.sprintlink.net!cpk-news-hub1.bbnplanet.com!atl-news-feed1.bbnplanet.com!news.bbnplanet.com!news.planetc.com!news.infonet.ee!212.7.1.68 test