From owner-biophysics@net.bio.net Mon Mar 01 22:00:00 1999 Path: biosci!pravda.ucr.edu!awabi.library.ucla.edu!208.134.241.18!newsfeed.cwix.com!198.138.0.5!newshub.northeast.verio.net!fu-berlin.de!vispars1.zib.DE!not-for-mail From: Hans-Christian Hege Newsgroups: bionet.biophysics,bionet.software Subject: Graduate Fellowship at ZIB, Berlin (Germany) Date: Tue, 02 Mar 1999 12:37:10 +0100 Organization: Konrad-Zuse-Zentrum (ZIB) Lines: 53 Message-ID: <36DBCD66.9056A0A4@zib.de> NNTP-Posting-Host: vispars1.zib.de (130.73.68.1) Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Access: 16 1224 1227 X-Trace: fu-berlin.de 920374630 4162 (none) 130.73.68.1 X-Mailer: Mozilla 4.5 [en] (X11; I; SunOS 5.5.1 sun4u) X-Accept-Language: en, de, de-DE, de-CH, de-AT Xref: biosci bionet.biophysics:4836 bionet.software:22969 Image Processing / Computational Geometry Graduate Fellowship Konrad-Zuse-Zentrum für Informationstechnik Berlin (ZIB) Konrad-Zuse-Zentrum für Informationstechnik Berlin (ZIB) invites students who are interested in pursuing a Ph.D. to apply for a fellowship in the Graduate Fellowship Program "Temperature Dependent Effects for Therapy and Diagnostics". This program is in close connection to the collaborate research centre 273 "Hyperthermia in Oncology: Methods and Clinic" at Humboldt University Berlin (speaker: Prof. Dr. R. Felix). The fellowship is available for two years with possibility of extension. The taxfree scholarship is DM 2.690,- per month for qualified students according to the regulations of the German Research Foundation (DFG). Research Areas: - algorithms for deforming anatomical grid models - algorithms for mapping grid models to medical image data - image analysis, registration and fusion Requirements: - above average masters degree (or diploma) in computer science, mathematics or physics - profound knowledge in image processing and/or computational geometry - good acquaintance with object oriented programming in C++ - interest in medical problems. We expect a high degree of commitment. In return we offer an interesting field of work, an opportunity for creativity, a highly motivated research team, excellent machinery equipment and favourable working conditions. We particularly encourage female applicants, since women are underrepresented in science and ZIB is engaged in enlarging the quota. Handicapped applicants will be preferred on terms of equal qualification. Applications with photo, curriculum vitae in tabular form and customary documents quoting the title "Graduiertenkolleg -- Scientific Visualization" should be submitted to Prof. Dr. Peter Deuflhard Konrad-Zuse-Zentrum für Informationstechnik Berlin (ZIB) Takustr. 7 14195 Berlin-Dahlem Germany For further information contact Dr. Martin Seebass (seebass@zib.de, phone: +49-30-84185-328) and see http://www.zib.de/Visual. From owner-biophysics@net.bio.net Tue Mar 02 22:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!news-feed.inet.tele.dk!bofh.vszbr.cz!news-lond.gip.net!news.gsl.net!gip.net!nntp.news.xara.net!xara.net!server6.netnews.ja.net!daresbury!not-for-mail From: SPhil79466@aol.com Newsgroups: bionet.biophysics Subject: Explosive New Marketing Strategy 800 607-6006 Ex 2492 Date: 3 Mar 1999 01:07:11 -0000 Organization: Daresbury Laboratory, Warrington, U.K. Lines: 34 Message-ID: <7bi1vv$sja$1@mserv2.dl.ac.uk> NNTP-Posting-Host: mserv2.dl.ac.uk content-length: 716 Return-Path: Apparently-To: This is a 1 time mailing Fire the Boss and break the Alarm Clock! I did! after eleven years, I left a $140,000 a year income for this opportunity I can truly say it was one of the best decisions of my life!!! Never commute again! make $2000-$5000 per week from home with a turn key marketing system! To find out how our system works call the 800#. 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Date: 3 Mar 1999 19:50:57 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 115 Sender: daemon@net.bio.net Distribution: world Message-ID: <6232ba4e.36ddf6e4@aol.com> NNTP-Posting-Host: net.bio.net TO REMOVE YOUR EMAIL ADDRESS, CALL 1 (800) 242-0363 #2155. --------------------------------------------------------------- IF YOU ARE SERIOUS ABOUT PROMOTING A PRODUCT, A SERVICE, A BUSINESS, OR A SITE ON THE NET, YOU NEED TO READ THIS MAILER! ================================================================== We offer an affordable range of unique softwares, services & products to help you to achieve your above objectives! ---------------------------------------------------------- 1)SOFTWARES: ___________ A)SENSOR PLUS!: a new & unique software that identifies a visitor to your site by detecting and grabbing the e-mail address of visitors to your site, and sending the visitors an email from you thanking them for the visit, and offering specials, etc. Try SENSOR PLUS! 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From owner-biophysics@net.bio.net Wed Mar 03 22:00:00 1999 Path: biosci!pravda.ucr.edu!awabi.library.ucla.edu!128.230.129.106!news.maxwell.syr.edu!howland.erols.net!news.idt.net!nntp.farm.idt.net!news From: "NDRI/HBDI" Newsgroups: alt.bio.technology,alt.bio.technology.misc,bionet,bionet.biology,bionet.biophysics,bionet.cellbiol Subject: Autopsy, Surgical Discard and Transplantation Tissues for Now Available for Research Date: Thu, 4 Mar 1999 08:22:34 -0500 Organization: IDT (Best News In The World) Lines: 16 Message-ID: <7bm1p4$koe@nnrp2.farm.idt.net> NNTP-Posting-Host: ppp-68.ts-1.ph.idt.net X-Newsreader: Microsoft Outlook Express 4.72.3155.0 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.3155.0 Xref: biosci bionet.biophysics:4840 bionet.cellbiol:11413 See www.ndri.com for access to human tissues and organs for medical researchers See www.hbdi.org for information about family collections containing DNA and immortalized cells - over 600 families - for medical and genetic researchers NDRI and HBDI are non-profit organizations aiding medical research and the search for disease genes. From owner-biophysics@net.bio.net Wed Mar 03 22:00:00 1999 Path: biosci!newshost.lanl.gov!awabi.library.ucla.edu!208.134.241.18!newsfeed.cwix.com!204.59.152.222!news-peer.gip.net!news-lond.gip.net!news.gsl.net!gip.net!nntp.news.xara.net!xara.net!server5.netnews.ja.net!daresbury!not-for-mail From: TopMan6304@aol.com Newsgroups: bionet.biophysics Subject: Serious? Date: 4 Mar 1999 03:46:00 -0000 Organization: Daresbury Laboratory, Warrington, U.K. Lines: 115 Message-ID: <7bkvlo$i0i$1@mserv2.dl.ac.uk> NNTP-Posting-Host: mserv2.dl.ac.uk content-length: 4895 Return-Path: Apparently-To: TO REMOVE YOUR EMAIL ADDRESS, CALL 1 (800) 242-0363 #2155. --------------------------------------------------------------- IF YOU ARE SERIOUS ABOUT PROMOTING A PRODUCT, A SERVICE, A BUSINESS, OR A SITE ON THE NET, YOU NEED TO READ THIS MAILER! ================================================================== We offer an affordable range of unique softwares, services & products to help you to achieve your above objectives! ---------------------------------------------------------- 1)SOFTWARES: ___________ A)SENSOR PLUS!: a new & unique software that identifies a visitor to your site by detecting and grabbing the e-mail address of visitors to your site, and sending the visitors an email from you thanking them for the visit, and offering specials, etc. Try SENSOR PLUS! 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You need a regular ISP internet connection. 2)EXCLUSIVE BULK MAILING: Promote your products and/or services! ______________________ - to 100,000 general addresses------------------- $195.00 - to 1/4 million general addresses--------------- 295.00 - to 1/2 million general addresses---------------- 495.00 - to 1 million general addresses--------------- 895.00 (An exclusive mailer is one where your 1.5 page ad is the ONLY one on the mailer! You do not share the mailer with anyone!) If you want to make use of the above offer, fax the Order Form below to (310) 495-0232. 3)CO-OP BULK MAILING: ____________________ Your 7-line ad will be one of 12 other ads in a mailer sent to 1 million general addresses. A line consists of 65 characters, spaces, etc.------$125.00 If you want to make use of this Special, fax the Order Form below to (310) 495-0232. 4)PACKAGE DEAL (#100): includes 4 marketing services as follows: ____________________ A)CO-OP MAILER: (service #2 above), B)SEARCH ENGINE SUBMISSION:submit your site to 1000+ search engines, dirctories, and FFAs, C)SUBMISSION TO 150 CLASSIFIED SITES ALL OVER THE WORLD, D)SUBMIT ELECTRONIC TRADE OPPORTUNITY TO INTER'L TRADERS. TOTAL COST OF PACKAGE:----------------------------------only $175.00 If you want to make use of this Package Deal, fax the Order Form below to (310) 495-0232. 5)9.5 MILLION EMAIL LISTS ON A CD! including 6 Mln Removal List $ 50.00 _______________________________ If you want to order the CD, fax the Order Form below to to (310) 495-0232. If you have any questions you want answered before ordering, please call (561) 732-9490, leave your name, number, the service/ product you want, and your question. You can also fax the above to (310) 495-0232. ====================================================================== ORDER FORM: To order any of the above services/product, fax your order, with credit card authorization, to (310) 495-0232. Please include: 1)Your name, address, phone number, fax number, and email address. This is needed for contact & shipping purposes only, and will not be given or sold to anyone! 2)Mention the products and/or services you want to order, & the Special Price quoted in this mailer. 2)Your Credit Card Number, expiry date, & amount you authorize us to charge to your card . Please sign the authorization. 3)Once we receive your fax, we shall contact you by phone or fax to discuss the order & get from you the information we need to perform the service or ship the product! ================================================================== TO REMOVE YOUR EMAIL ADDRESS, CALL (800) 242-0363 EXTENSION #2155. From owner-biophysics@net.bio.net Wed Mar 03 22:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!news.gtei.net!firehose.mindspring.com!user-38h1tg9.dialup.mindspring.com!user From: prevelig@uab.edu (Peter Prevelige) Newsgroups: bionet.biophysics Subject: Postdoctoral Postion Protein/Protein Interactions Date: Thu, 04 Mar 1999 07:45:51 -0600 Organization: Univ. of Alabama at Birmingham Lines: 17 Message-ID: NNTP-Posting-Host: d1.10.f6.09 X-Server-Date: 4 Mar 1999 13:44:38 GMT A postdoctoral position is available immediately in the laboratory of Peter Prevelige, Dept. of Microbiology, Univ. of Alabama at Birmingham to study protein/protein interactions during viral capsid assembly. The overall objective of the project is to develop a computer simulation of the process of capsid assembly based on experimentally obtained data. The experimental component of the project includes direct measurment of key protein/protein interactions using state of the art techniques including: surface plasmon resonance, calorimetric, small angle X-ray scattering and analytical ultracentrifugation. Candidates should have experience or interest in protein biochemistry and the kinetic and thermodynamic analysis of protein/protein interactions. For more information, please visit http://www.microbio.uab.edu/faculty/Prevelige/prevelige-p.htm or email Peter Prevelige prevelig@uab.edu From owner-biophysics@net.bio.net Thu Mar 04 22:00:00 1999 Path: biosci!HOTMAIL.COM!qmesquita From: qmesquita@HOTMAIL.COM ("Quintiliano H. de Mesquita") Newsgroups: bionet.biophysics Subject: Myogenic Theory Explains! Date: 5 Mar 1999 03:37:13 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 394 Sender: daemon@net.bio.net Distribution: world Message-ID: <36DFBEE5.FAB3EDC1@hotmail.com> NNTP-Posting-Host: net.bio.net Myogenic Theory Explains! “The Thrombogenic Theory of Myocardial Infarction Tumbled Down and the Ortodoxy Didn’t Have Noticed” The ortodox cardiology stay repressed in their therapeutic behaviour in front of coronary- myocardiopathy, since when the thrombogenic theory (TT) started to tumble down after 25 years of clinical research (1944-69), with the recognition about the failure of coumarin and heparin anticoagulants to stop the unstable angina (UA) and in prevention of myocardial infarction (MI). During 10 years (1944-54) we have participated in clinical studies about anticoagulants, recording its failure to stop the UA and in the MI prevention, what led us to reject such therapeutic agents. In 1969, with the general admission of the anticoagulants failure, happened the introduction of the coronary bypass surgery practiced directly in man, without the previous assessment in experimentation animals. Consequently, heart surgeons and cardiologists with no other visible way to manage chronic coronary-myocardiopathy and acute coronary syndromes, have assumed together the compulsive intervencionism practiced during the last 3 decades: myocardial reperfusion through angioplasty and/or coronary bypass surgery. At the same time they indicate coronary dilators, antiaggregate of platelets and beta blockers. Beta blockers, in our point of view, represents a contradictory behaviour by the cardiologists in front of the contractile deficient condition of the dependent coronary myocardial segment, frequently developing generalized hypotonia. The only actual concern by the ortodox cardiologist is to provide the myocardial reperfusion in any case, independently of sex or age, without any future guarantee regarding the success of the reperfusion act, generally submitted to the repetition practice due to its frequent failure. In 1972, we developed the myogenic theory (MT) stating the acute myocardial infarction (AMI) as the cause and not consequence of coronary thrombus. The myogenic theory met important support in pathological anatomy findings since 1950 decade where the authors preconized the coronary thrombus as consequence of acute myocardial infarction and not its cause. According the myogenic theory concepts the AMI is developed by functional degradation state of the regional myocardial coronary-dependent characterized by a pathophysiology essencially myogenic, in the 3 stages of coronary-myocardiopathy: - The stable angina with or w/out previous myocardial infarction, developed by exertion or emotion and producing regional ventricular ischemia (RVI), coronary-dependent, in which the negative inotropic action develops the regional myocardial insufficiency (RMI), reciprocal, ceasing with the stop of the provoking cause. - The unstable angina (UA) as a spontaneous, reversible and recurrent syndrome resulted from an abrupt, and unexpected RMI followed by RVI of long duration and resistant to all available therapeutic agents. - The UA is perpetuated in the last crises of angina with its transformation in infarctioning clinical picture (ICP) represented by RMI + RVI -> myocardial infarction -> myocardial necrosis -> coronary thrombosis not obligatory; electrocardiographically recognized as myocardial infarction with Q wave and w/out Q wave. In front of this pathophysiological mechanism the cardiotonic appeared as a new therapeutic concept and since 27 years ago proves to be capable to preserve the myocardial and symptomatic stability in the stable angina pectoris with or w/out previous infarction, to stop the UA and to provide the transformation of ICP in avoided myocardial infartion, ICP- interrupted myocardial infarction or characterized as ICP-infarcted according the behaviour of enzymatic peaks. In 1981 the thrombogenic theory suffered the final tumble down when the thrombolytics which were reintroduced in the UA and AMI showed that they are uneffectives regarding the clinical events in the UA and passed to be indicated only in the treatment of AMI. During the last 2 decades has been clearly demonstrated in vast literature that, thrombolytics and angioplasty release the coronary arteries related with the MI, while is recorded the coronary/dependent ventricular dysfunction process. Gregorini, L et al, in a recent article (Circulation, 1999; 99:482-90), besides to confirm such aspects of left ventricular dysfunction after thrombolysis and coronary angioplasty, stressed the role of alpha-adrenergic blockers in reducing the coronary vasoconstriction and improving the left ventricular dysfunction post-ischemic. In the myogenic theory point of view, the left ventricular dysfunction recorded in this way is preconized as a primary process. Also, the paper from Tamura, K et al (Am Heart J, 1996; 131:731-5) reported about successive echocardiographical and electrocardiographical recordings in UA showing myocardial aspects of RMI + RVI which concepts are inside of the MT; If they had applied the cardiotonic during their study, they certainly had the record of immediate clinical and physiological stop of UA. We need to emphasize the paper from Murakami, T et al (Am J Cardiol, 1998; 82 :839-44) about its new methodology represented by aspiration thrombectomy after thrombolysis showing that intracoronary thrombus is absent in a substantial number of patients with acute myocardial infarction. We have the impression that the TT once more have lost its support about the coronary thrombosis in the AMI when these authors came to strenghten our concept of primary myocardial infarction and secondary coronary thrombosis. Their findings after thrombolysis indicate: Recent thrombus (49%), Without thrombus (30%), Atheroma (14%), Organized thrombus (2%) and Not defined thrombus (5%). No doubts that all ways go to the complete demonstration that cases normally submitted to angiographic and ventriculographic studies arrive with the recognition of conflictant aspects with the TT and compatible aspects with the MT. Therefore, we will pass in review such aspects that must be considered as the pathological reality of coronary-myocardiopathy which the Myogenic Theory Explains: - Roberts, WC (Circulation, 1972;49:1) showed that in cases of AMI with early sudden death that coronary thrombosis ocurred in approximately 10% of cases with subendocardial infarction of left ventricle and in around 50% of cases with transmural necrosis. MT explains: the recent findings by Murakami et al during the acute myocardial infarction, confirmed and reinforced old pathological studies realized in the 1950 decade which were stressed by Roberts in 1972. - Spain, DM and Bradess, VA (Am J Med Sci, 1960; 240:701) showed total coronary obstruction of atherosclerotic nature representing around of 75% of cases of AMI and only 25% of cases with coronary thrombosis in autopsy, recorded during 25 years. MT explains: in these cases the total coronary atherosclerotic obstruction was old and the AMI was resulting from severe regional myocardiopathy coronary-dependent with MRI + RVI and secondary coronary thrombosis not obligatory. - Spain, DM and Bradess, VA (Circulation, 1960; 22: 816) recorded the increase of incidence of coronary thrombosis related with the increase of lifetime after AMI: < 1 hour = 16%, 1-14 hours = 37% and > 24 hours = 53%. MT explains: these numbers represent a clear demonstration that AMI is primary and the coronary thrombosis secondary, not obligatory and with slow formation. - Erhardt, LR et al (Lancet, 1973; 1:387; Am Heart J, 1976; 91:592) demonstrated the coronary thrombosis incorporating fibrinogen marked by I-125 and I-131, radioactive agents administered after 10-15 hours from the start of the clinical manifestations of AMI, fact that suggests coronary thrombosis as consequence of primary myocardial necrosis; and the record of the exclusion of I-125 in the thrombus of 1 case, when the radioactive agent was administered 47 hours after the start of clinical manifestations of AMI. MT explains: the radioactive agents I-125 and I-131 incorporated to the fibrinogen in the thrombus in progressive organization, not happened in the referred 1 case because the thrombus was already formed. - Hellstrom, HR (Circulation, 1970; 42 (Suppl III) : 165) demonstrated in a experimental manner, that AMI produced by surgical ligature of coronary artery without endothelial lesion was responsible by the vascular stasis and the consequent coronary thrombosis, recorded in the coronary artery after released. MT explains: significant coronary thrombosis formed as consequence of vascular stasis in the artery blocked by the AMI; such process is similar to the cerebral and cardiac infarctions coincident with the use of anticonceptives. - AMI coincident with rupture of atheromatous plaque and coronary thrombosis from coronary artery related with the infarcted region. MT explains: the coronary thrombosis watched in this way have been conveniently interpreted by the ortodoxy as primary. However, the coronary thrombosis can come late, subsequently to the AMI, whose rupture of the plaque should be provoked by the invasion of white blood cells identified as inflamatories and by its derived products providing the formation of coronary thrombosis. - Coronary arteries angiographically normal related with the infarction area without coronary thrombosis. MT explains: these arteries are widely compromised by the atherosclerotic process, exclusive of wall, apparently canalized but inelastic, what modifies the circulation from continuous to intermitent type which will endanger the dependent myocardium, taking it to the AMI according the MT model. - Case of UA interrupted by the cardiotonic, showing normal coronary arteries with slow flow and defined asynergy of the myocardial region coronary dependent and with systolic residual volume increased > (+/++); which, in the period of 2 years (still using cardiotonic), evolved to the total obstruction of the left descendent anterior artery and ventriculogram identical with systolic residual volume > (++), but without worsening the clinical situation. MT explains: the use of cardiotonic + coronary dilator guaranteed the preservation of myocardial and symptomatic stability, in spite of the evolution of the coronary arterial process to the total obstruction without evolving to the MI. - Bulkley, BH and Hutchins, GM (Circulation, 1977;56:906) published cases of AMI situated in revascularized region by pervious coronary artery bypass, interpreted as paradoxal infarction. MT explains: in these cases the myocardial ischemic effects exist and the ventriculographic aspects are useful to demonstrate that AMI is primary, even without coronary thrombosis which we consider not obligatory. - Old atherosclerotic plaque totally obstructing the coronary artery related with the infarcted region. MT explains: cases of this type are useful to demontrate that the compromised coronary-dependent myocardium is taked to the RMI + RVI and to the AMI by evolutive and degradated coronary-myocardiopathy process. - AMI developed during the habitual or unusual practice of physical activities during or immediate after the ergometric test, sex, sport or during the digestive period plus physical efforts (even light), frequently happens in front of coronaries with not significant, severe or even total obstructions by old plaques, simultaneous or not with coronary thrombosis. MT explains: in that cases the dependent coronary myocardial region compromised in its structure can arrive to the AMI through an abrupt exhaustion of the myocardial region – RMI + RVI – followed by coronary thrombosis. - The occurrence of AMI within 2-21 days after the abrupt interruption of beta blockers, in continued use. MT explains: due to its negative inotropic effect beta blockers develop generalized hypotonia which eliminate the intersegmentary confrontation of the coronary/dependent myocardium, with the effect of temporization but without to avoid the AMI neither other complications like cardiac insufficiency and sudden death. Its abrupt interruption is followed by the reestablishment of the intersegmentar confrontation owing to the unprepared coronary/dependent myocardial region which takes to the UA with manifestations of RMI + RVI, occurrence of AMI, cardiac insufficiency, severe arrhythmias and sudden death. - Occurrence of AMI after the interruption of cardiotonic in continued use for the preservation of the myocardial and symptomatic stability in chronic coronary-myocardiopathy with or without previous myocardial infarction. MT explains: the cardiotonic absence provides the myocardial intersegmentar confrontation which may lead to the UA and consequently to the AMI, according the model preconized by the MT. - Case of UA interrupted by the cardiotonic, showing the 3 coronaries - left descendent anterior, right and circunflex – completely obstructed, but with a rich net of collateral coronary, and left ventricular dysfunction but without recordings of anterior infarction. MT explains: This case came to confirm more one time the immediate results of interruption of UA in 100% of the cases and without mortality, with the use of the new therapeutical concepts of the MT. Treatment of attack followed by the permanently upkeeping with the cardiotonic + coronary dilator which develops the return to the myocardial stability preserved in this way and as prevention of myocardial infarction. - Case of angina, permanent and with great suffering with repeated crises of UA, resistant to all types of medication, lasting 9 months; happening 10 years after the myocardial infarction with total obstruction of the 3 epicardial coronaries – DAE, D and CFx – ventriculogram with large increase of the cardiac area and characteristics of hibernating Heart. He was attended in our coronary care unit during the UA crisis when was submitted to the cardiotonic, recording immediate and complete relief. From this moment on this patient remained under treatment with cardiotonic in a myocardial and symptomatic stability during 13 years of comfortable survival, passing away at 73 years old, due to a cerebral stroke. MT explains: this case was interpreted as permanent deficient myocardial condition mixed with symptomatic instability represented by crises of RMI + RVI finally interrupted by the cardiotonic and therefore upkeeped for long survival. - Cases of chronic stable angina with the symptomatic and myocardial stability preserved by decades, with or w/out previous myocardial infarction showing evolutive coronary atherosclerotic processes to the total obstruction of 2-3 coronaries w/out occurrence of AMI. MT explains: maintained by the cardiotonic + coronary dilator therapeutic since 1991 with the addition of ACE inhibitor, his future has been calm and guaranteed, complementing in this way the effects provided by the coronary collateral circulation. - Cases of UA interrupted by the cardiotonic, maintained by decades, of patients with significant obstructions of 2-3 coronaries have had evolution to total obstruction with rich net of collateral coronary, without recordings of myocardial infarction, during the long survival. MT explains: Interruption of UA by cardiotonic as attack and maintenance treatment have guaranteed the preservation of myocardial and symptomatic stability as well in the prevention of myocardial infarction. - Cases generally admitted in coronary care units as with AMI were treated by cardiotonic in our unit, showing clinical and enzymatic transformations which led us to classify them as clinical infarctioning pictures, determined by the behaviour of enzymatic peaks. MT explains: the cardiotonic in AMI was considered as a myocardial protector (Mesquita, 1973; Pizzarello et al, 1975 and Morrison et al, 1976.1980). Through the enzymatic peaks the cases observed by us were distinguished in tyhe following way: ? Myocardial infarction avoided: 20% of cases with normal enzymatic peaks or < 2x the normal. ? Infarctioning clinical picture- interrupted: 47% of cases with enzymatic peaks < 3x the normal. ? Infarctioning clinical picture- infarcted: 33% of cases with enzymatic peaks > 3x the normal. - The method of Murakami et al using intracoronary thrombectomy aspiration and their findings showing absence of coronary thrombus during acute myocardial infarction and the use of transesophageal echocardiogram for the same purpose. MT explains: the aspiration thrombectomy with thrombolysis and the echocardiography can serve as support to the myogenic theory and keep away forever the TT in vivo, offering to the pathologists the prove that coronary thrombus is secondary. Serve also as a response to the paper of Chandler et al (AM J Cardiol, 1974; 34:823) about coronary thrombosis and AMI simultaneous, indicating what is primary and secondary. In Murakami method the thombolysis release the artery related with the myocardial infarction while the aspiration thombectomy cleans the artery, stressing the left ventricular dysfunction which can be corrected by the cardiotonic or by using the alpha-adrenergic blocker as Gregorini et al. - Important findings in the acute UA about the incidence of intracoronary thrombus (52-85%) few hours after the start of symptoms until 2 weeks, while its incidence in the chronic phase has been always low (0-12%). MT explains: the echocardiography during the UA clearly shows the myogenic mechanism recording the RMI which develops the RVI characterized by ECG, coincident with platelet aggregates and inflammatory blood cells which invade the involved region, becoming to the normal with the stop of crises. - Ambrose et al (Am J Cardiol, 1988;61:244-7) considered the myocardial infarction without Q wave as an intermediary stage from UA to the myocardial infarction with Q wave. They recorded total coronary obstruction in 26% of cases w/out Q wave and in 90% of cases with Q wave. MT explains: the myocardial infarction w/out Q wave as intermediate stage to the myocardial infarction with Q wave must be seen as favourable to the MT, and its mechanism is considered by us as the preconized for the UA of long duration and in smaller level than the reached by the AMI. So, in this case the myocardial lesion is less stressed in subendocardial or subepicardial layers, and with low incidence of coronary thrombosis. - DeWood, MA et al (NEJM, 1986; 315:417-23) recorded total coronary obstruction in 32% of AMI cases without Q wave and in a more detailed study showed an incidence for total coronary obstruction significantly and progressively increased in relation with the execution time of angiographic examination: 26% in 24 hours, 37% from 24-72 hours and 42% from 72 hours to 7 days. They also have recorded that the incidence of subtotal coronary obstruction (> 90%), in myocardial infarction without Q wave, showed evident reduction in gradual manner: 34% in 24 hours, 26% from 24-72 hours and 18% from 72 hours to 7 days. MT explains: These crescent indicators show that the formation of coronary thrombus is secondary to the AMI depending directly of the evolution time of the infarctioning process to become infarcted process; Evidently, the gradual reduction of indicators referring to subtotal obstruction was observed contrary and parallel to the thrombotic transformations recorded secondarily to the infarction. - Thrombolytics in UA improve the arterial events but show inefficiency about the clinical events and regarding to stop the pathophysiological process, what led investigators to recommend its use only in AMI. (Rentrop, P et al, 1981, Williams, DO et al, 1990, Leinbach, RC, 1972, Freeman, MR et al, 1992, TIMI IIIA Investigators, 1993 and Chen, L et al, 1997) MT explains: These results came to confirm the failure of anticoagulants (1969) and completed the tumble down of the TT what the ortodox cardiology make believe that do not happens. - Roberts, WC (Am J Cardiology, 1984; 97:1410) wrote: "When I have an acute myocardial infarction take me to the hospital that has a cardiac catheterization laboratory and open cardiac surgical facilities". MT explains: in case of chronic coronary-myocardiopathy the preservation of myocardial and symptomatic stability and prevention of myocardial infarction have been guaranteed by the association of cardiotonic + coronary dilator + ACE inhibitor, permanently. So, my advice to all them which think like the grand teacher of pathological anatomy represented by William C. Roberts, to defend their heart with calm using the therapeutical efficacy by cardiotonics which complement the providential action of the collateral coronary circulation. Quintiliano H. de Mesquita, M.D. For more information please visit my homepage at http://www.infarctcombat.org/qhm/homepage.html From owner-biophysics@net.bio.net Thu Mar 04 22:00:00 1999 From: "Clive Delmonte" Newsgroups: bionet.biophysics,bionet.xtallography Subject: DNA Structure: Puzzle #1 (Version 2) Date: Fri, 5 Mar 1999 09:38:19 -0000 Lines: 3810 X-Newsreader: Microsoft Outlook Express 4.72.3110.5 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.3110.3 NNTP-Posting-Host: 195.7.227.153 Message-ID: <36dfa26d.0@news.netdirect.net.uk> X-Trace: 5 Mar 1999 09:22:53 GMT, 195.7.227.153 Path: biosci!bloom-beacon.mit.edu!news.kodak.com!news-nysernet-16.sprintlink.net!news-east1.sprintlink.net!-program!news-peer1.sprintlink.net!news.sprintlink.net!news-peer.gip.net!news-lond.gip.net!news.gsl.net!gip.net!newspeer.clara.net!news.clara.net!peer.news.nildram.co.uk!news-peer.netdirect.net.uk!news.netdirect.net.uk!195.7.227.153 Xref: biosci bionet.biophysics:4844 bionet.xtallography:4641 Microprobe Studies of DNA Give New Insights into Duplex Structure In their STM study of nucleic acids (1), Lee et al. recorded in Figure 3(b) lengthy stretches of at least four well-resolved linear DNA duplexes. The vital feature of this image of DNA molecules is that no plectonaemic winding can be discerned. That is, they show no sign of being double-helical. The duplexes consist of long, paired, helices with a consistent, uninterrupted contour running between the two strands of each of the four duplexes, whose highlighted features remain exactly in phase with each other over their whole lengths, extending to some 17 - 20 full helical turns. In their Figure 3d is shown the height of the duplex, that is, its diameter as it lies on the substrate, equal to 1.3 nm. Their images accord with the sketch of B-DNA shown in the attachment (2, page 48) for which the cross sectional, helical dimensions are determined solely by the width of the Watson-Crick base pair, namely 1.1 nm, connected across the helices, while the pitch remains at 3.34 nm. In Ref. 2 (pages 51, 149, 170) there are details of three independent demonstrations reported by different researchers, deducible from experimental work in the literature, that Isomer 2 is that which is actually found in DNA in vivo, and that Isomer 1 is unknown. -------------------------------------------------------------- 1 Scanning Tunnelling Microscopy of Nucleic Acids; G. Lee, P.G. Arscott, V.A. Bloomfield & D. Fennell Evans; SCIENCE Vol 244 (1989) 475 - 477 2 Towards A New Structural Molecular Biology, by Clive Delmonte, ISBN 0 9512276 0 2 (1991) -------------------------------------------------------------- Clive Delmonte begin 666 SBSDUPLEXBMPR.bmp M0DUJE0(``````#X````H````SP,``%4%```!``$``````"R5`@!#%P``0Q<` M``(````"`````````/___P#_____________________________________ M____________________________________________________________ M____________________________________________________________ M_____@``____________________________________________________ M____________________________________________________________ M__________________________________________________X``/______ M____________________________________________________________ M____________________________________________________________ M___________________________________^``#_____________________ M____________________________________________________________ M____________________________________________________________ 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