From owner-biophysics@net.bio.net Sat May 01 22:15:00 1999 From: francis_kuttner@hotmail.com Newsgroups: sci.research.postdoc,bionet.biophysics,bionet.cellbiol,bionet.cellbiol.cytonet Subject: radiobiology Date: Sat, 01 May 1999 23:00:24 GMT Organization: Deja News - The Leader in Internet Discussion Lines: 28 Message-ID: <7gg128$l6b$1@nnrp1.dejanews.com> NNTP-Posting-Host: 128.148.185.143 X-Article-Creation-Date: Sat May 01 23:00:24 1999 GMT X-Http-User-Agent: Mozilla/4.51 [en] (Win95; I) X-Http-Proxy: 1.0 x1.dejanews.com:80 (Squid/1.1.22) for client 128.148.185.143 Path: biosci!news.ic.sunysb.edu!news-nysernet-16.sprintlink.net!news-east1.sprintlink.net!news-peer1.sprintlink.net!news.sprintlink.net!news.maxwell.syr.edu!nntp2.dejanews.com!nnrp1.dejanews.com!not-for-mail Xref: biosci bionet.biophysics:4965 bionet.cellbiol:11730 bionet.cellbiol.cytonet:1558 dear people, i am a graduate student at tufts university and i am very much interested in radiobiology and radition chemistry. currently i am looking for a research laboratory that would be interested to accomodate me and sponsor my research. i have narrowed my focus in radiobiology to apoptosis in mouse lymphocytes. and i am wondering if any of you would comment on the possible research questions that i have come across. please email me at francis_kuttner@hotmail.com if you have any ideas concerning my research topics or forward this post to anyone you know who is interested in hiring! thanks in advance!! 1. normal mouse lymphocytes (thymocytes) show that normally, under unstressed conditions, a large fraction of the total lymphocyte population can be identified as apoptotic cells. 2. cells showing large extents of apoptosis that are generally more radiosensitive than cells showing smaller extents of apoptosis. 3. as dosage increases, the increase in percentage of apoptotic cells is essentially the same in the neutron or photon irradiated cells. 4. there clear evidence of a G2 block after the 3 Gray doses. 5. it cannot be determined if there is a G1 block because the decrease in the height of the G1 peak may simply reflect cells becoing apoptotic rather than progressing into S phase. 6. there is clear evidence of a large Q cell population in the control cells. 7. the km for the dose response curves is about 45 rad for both curves. ~francis -----------== Posted via Deja News, The Discussion Network ==---------- http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own From owner-biophysics@net.bio.net Mon May 03 06:54:00 1999 Path: biosci!WSUNIX.WSU.EDU!arthurr From: arthurr@WSUNIX.WSU.EDU (art roberts) Newsgroups: bionet.biophysics Subject: New Site with Biophysics Links Date: 3 May 1999 00:54:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <372D55D6.96F23092@wsunix.wsu.edu> NNTP-Posting-Host: net.bio.net http://biotech.isCool.net This site allows easy access to hyperlinks and free software for the biochemist, biophysicist and molecular biologist. This site is constantly evolving and expanding, so it can be easy to use, reliable, and comprehensive. This a purely a non-profit site for your enjoyment. Sincerely, Art Roberts (web designer) From owner-biophysics@net.bio.net Mon May 03 11:38:00 1999 Path: biosci!rutgers!rockyd.rockefeller.edu!newsfeed.nyu.edu!news.idt.net!nntp.farm.idt.net!news From: "NDRI/HBDI" Newsgroups: alt.bio.technology,alt.bio.technology.misc,bionet,bionet.biology,bionet.biophysics,bionet.cellbiol Subject: Human Tissues, Organs and DNA from Families for Medical Research Date: Mon, 3 May 1999 08:27:43 -0400 Organization: IDT (Best News In The World) Lines: 14 Message-ID: <7gk4of$6m9@nnrp2.farm.idt.net> NNTP-Posting-Host: ppp-11.ts-1.ph.idt.net X-Newsreader: Microsoft Outlook Express 4.72.3155.0 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.3155.0 Xref: biosci bionet.biophysics:4967 bionet.cellbiol:11739 See www.ndri.com for access to human tissues and organs for medical researchers See www.hbdi.org for information about family collections containing DNA and immortalized cells - over 600 families - for medical and genetic researchers NDRI and HBDI are non-profit organizations aiding medical research and the search for disease genes. From owner-biophysics@net.bio.net Mon May 03 17:50:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!msunews!nntp.upenn.edu!mail.med.upenn.edu!jirong From: jirong@mail.med.upenn.edu (Jirong Liu) Newsgroups: bionet.biophysics,bionet.microbiology,bionet.neuroscience,bionet.general Subject: Re: Reviews of Science Followup-To: bionet.biophysics,bionet.microbiology,bionet.neuroscience,bionet.general Date: 3 May 1999 18:43:36 GMT Organization: University of Pennsylvania Lines: 16 Message-ID: <7gkqoo$ca$1@netnews.upenn.edu> References: <7gcaqk$m6t$1@nnrp1.dejanews.com> NNTP-Posting-Host: mail.med.upenn.edu X-Newsreader: TIN [version 1.2 PL2-upenn1.1] Xref: biosci bionet.biophysics:4968 bionet.microbiology:16569 bionet.neuroscience:29167 bionet.general:33046 Jesper E. Mogensen (lime@mail1.stofanet.dk) wrote: : Please go to : http://ajanta.sci.ccny.cuny.edu/~jupiter/pub/review/index.html : to read some book reviews that teach, inform and enlighten one : about science. : Topics currently covered are : (1) The lastest developments in evolution : (2) Cosmology : (3) Fundamental physics : (4) Black holes and relativity : -----------== Posted via Deja News, The Discussion Network ==---------- : http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own From owner-biophysics@net.bio.net Tue May 04 16:05:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!news-peer1.sprintlink.net!news.sprintlink.net!news.maxwell.syr.edu!nntp2.dejanews.com!nnrp1.dejanews.com!not-for-mail From: francis_kuttner@hotmail.com Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.cellbiol.cytonet,bionet.general Subject: Cell Population Kinetics Date: Tue, 04 May 1999 16:49:20 GMT Organization: Tufts University Sackler School of Graduate Biomedical Sciences Lines: 19 Message-ID: <7gn8ef$ghu$1@nnrp1.dejanews.com> Reply-To: francis_kuttner@hotmail.com NNTP-Posting-Host: 128.148.185.143 X-Article-Creation-Date: Tue May 04 16:49:20 1999 GMT X-Http-User-Agent: Mozilla/4.51 [en] (Win95; I) X-Http-Proxy: 1.0 x3.dejanews.com:80 (Squid/1.1.22) for client 128.148.185.143 Xref: biosci bionet.biophysics:4970 bionet.cellbiol:11743 bionet.cellbiol.cytonet:1561 bionet.general:33051 Hi, I posted a message a few days ago concerning my potential research topics in radiobiology and radiation chemistry, and I have heard from a few people. Thanks! I am currently brushing up on my knowledge of cell population kinetics but in this review I am encountering some trouble. If anyone can help please write me at francis_kuttner@hotmail.com. Thank you. ~Francis 1. Why are oxic cells more radiosensitive to apoptosis than anoxic cells? 2. Why do radiosensitizing effects of oxygen disappear at high doses of radiation? 3. Deletion of the Rb gene in myc. Decreases survival? Increases apoptosis? 4. Deletion of the cyclin D gene in ras cells. Decreases survival? Decreases apoptosis? 5. Overexpression of the cyclin B gene in ras and myc cells. Same survival as in controls? Increases apoptosis? 6. From the viewpoint of effects on apoptosis, a decrease in the cellular supply of ATP should decrease cell survival? 7. Why does the repair saturation model as presented mathematically and experimentally fail as a comprehensive model of cell killing? -----------== Posted via Deja News, The Discussion Network ==---------- http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own From owner-biophysics@net.bio.net Tue May 04 16:55:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!netnews.com!newspeer.monmouth.com!nntp2.dejanews.com!nnrp1.dejanews.com!not-for-mail From: francis_kuttner@hotmail.com Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.cellbiol.cytonet,bionet.general Subject: Cell Population Kinetics Date: Tue, 04 May 1999 17:44:13 GMT Organization: Tufts University Sackler School of Graduate Biomedical Sciences Lines: 19 Message-ID: <7gnblc$joo$1@nnrp1.dejanews.com> Reply-To: francis_kuttner@hotmail.com NNTP-Posting-Host: 128.148.185.143 X-Article-Creation-Date: Tue May 04 17:44:13 1999 GMT X-Http-User-Agent: Mozilla/4.51 [en] (Win95; I) X-Http-Proxy: 1.0 x13.dejanews.com:80 (Squid/1.1.22) for client 128.148.185.143 Xref: biosci bionet.biophysics:4971 bionet.cellbiol:11744 bionet.cellbiol.cytonet:1562 bionet.general:33052 Hi, I posted a message a few days ago concerning my potential research topics in radiobiology and radiation chemistry, and I have heard from a few people. Thanks! I am currently brushing up on my knowledge of cell population kinetics but in this review I am encountering some trouble. If anyone can help please write me at francis_kuttner@hotmail.com. Thank you. ~Francis 1. Why are oxic cells more radiosensitive to apoptosis than anoxic cells? 2. Why do radiosensitizing effects of oxygen disappear at high doses of radiation? 3. Deletion of the Rb gene in myc. Decreases survival? Increases apoptosis? 4. Deletion of the cyclin D gene in ras cells. Decreases survival? Decreases apoptosis? 5. Overexpression of the cyclin B gene in ras and myc cells. Same survival as in controls? Increases apoptosis? 6. From the viewpoint of effects on apoptosis, a decrease in the cellular supply of ATP should decrease cell survival? 7. Why does the repair saturation model as presented mathematically and experimentally fail as a comprehensive model of cell killing? -----------== Posted via Deja News, The Discussion Network ==---------- http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own From owner-biophysics@net.bio.net Fri May 07 19:15:00 1999 Path: biosci!rutgers!rockyd.rockefeller.edu!news-nysernet-5.sprintlink.net!news-east1.sprintlink.net!news-peer-europe.sprintlink.net!news.sprintlink.net!netnews.com!news.maxwell.syr.edu!news-peer.gip.net!news.gsl.net!gip.net!news.idt.net!nntp.farm.idt.net!news From: "NDRI/HBDI" Newsgroups: alt.bio.technology,alt.bio.technology.misc,bionet,bionet.biology,bionet.biophysics,bionet.cellbiol Subject: Autopsy, Surgical Discard and Transplantation Tissues for Now Available for Research Date: Fri, 7 May 1999 15:58:46 -0400 Organization: IDT (Best News In The World) Lines: 16 Message-ID: <7gvguj$b9a@nnrp2.farm.idt.net> NNTP-Posting-Host: ppp-2.ts-1.ph.idt.net X-Newsreader: Microsoft Outlook Express 4.72.3155.0 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.3155.0 Xref: biosci bionet.biophysics:4973 bionet.cellbiol:11759 See www.ndri.com for access to human tissues and organs for medical researchers See www.hbdi.org for information about family collections containing DNA and immortalized cells - over 600 families - for medical and genetic researchers NDRI and HBDI are non-profit organizations aiding medical research and the search for disease genes. From owner-biophysics@net.bio.net Mon May 10 12:13:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!news-feed.inet.tele.dk!bofh.vszbr.cz!news.belnet.be!not-for-mail From: Guy Droogmans Newsgroups: bionet.software,bionet.biophysics Subject: analysis software for pClamp data Date: Mon, 10 May 1999 14:58:34 +0200 Organization: KULeuvenNet Lines: 80 Message-ID: <3736D7FA.EE421ACB@med.kuleuven.ac.be> NNTP-Posting-Host: marvin.kulnet.kuleuven.ac.be Mime-Version: 1.0 Content-Type: multipart/alternative; boundary="------------836BA5E8AE12E2C4C751C06B" X-Trace: naxos.belnet.be 926341690 11886 134.58.127.3 (10 May 1999 13:08:10 GMT) X-Complaints-To: abuse@belnet.be NNTP-Posting-Date: 10 May 1999 13:08:10 GMT X-Mailer: Mozilla 4.51 [en] (Win98; I) X-Accept-Language: en Cache-Post-Path: marvin!unknown@n36pc34.med.kuleuven.ac.be X-Cache: nntpcache 2.3.3 (see http://www.nntpcache.org/) Xref: biosci bionet.software:23423 bionet.biophysics:4975 --------------836BA5E8AE12E2C4C751C06B Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit WinASCD is windows-32 program fro analyzing whole-cell and single channel currents acquired with pClamp software in triggered or continous sampling modes (Clampex, Fetchex, Axotape). Features 1. data correction procedures * baseline correction for removing slow drift * background correction * linear leak and capacitive transient correction 2. analysis * extract peak and average currents from traces * compose a record from a single or multiple files * ensemble averaged current * fit current traces: polynome, sum of exponentials, Hodgkin-Huxley kinetics, kinetic scheme * analysis of current variance * single channel: amplitude histogram, open probability, mean open time, construct and analyze idealized current traces. --------------836BA5E8AE12E2C4C751C06B Content-Type: text/html; charset=us-ascii Content-Transfer-Encoding: 7bit WinASCD is windows-32 program fro analyzing whole-cell and single channel currents acquired with pClamp software in triggered or continous sampling modes (Clampex, Fetchex, Axotape).
Features
1. data correction procedures
  • baseline correction for removing slow drift
  • background correction
  • linear leak and capacitive transient correction
2.  analysis
  • extract peak and average currents from traces
  • compose a record from a single or multiple files
  • ensemble averaged current
  • fit current traces: polynome, sum of exponentials, Hodgkin-Huxley kinetics, kinetic scheme
  • analysis of current variance
  • single channel: amplitude histogram, open probability, mean open time, construct and analyze idealized current traces.


 
 
  --------------836BA5E8AE12E2C4C751C06B-- From owner-biophysics@net.bio.net Tue May 11 12:38:00 1999 From: "NDRI/HBDI" Newsgroups: alt.bio.technology,alt.bio.technology.misc,bionet,bionet.biology,bionet.biophysics,bionet.cellbiol Subject: Autopsy, Surgical Discard and Transplantation Tissues for Now Available for Research Date: Tue, 11 May 1999 08:40:09 -0400 Organization: IDT (Best News In The World) Lines: 16 Message-ID: <7h98f3$f5j@nnrp4.farm.idt.net> NNTP-Posting-Host: ppp-33.ts-1.ph.idt.net X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2314.1300 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2314.1300 Path: biosci!news.ic.sunysb.edu!news-nysernet-16.sprintlink.net!news-east1.sprintlink.net!news-peer1.sprintlink.net!news.sprintlink.net!news-peer.gip.net!news.gsl.net!gip.net!news.idt.net!nntp.farm.idt.net!news Xref: biosci bionet.biophysics:4976 bionet.cellbiol:11780 See www.ndri.com for access to human tissues and organs for medical researchers See www.hbdi.org for information about family collections containing DNA and immortalized cells - over 600 families - for medical and genetic researchers NDRI and HBDI are non-profit organizations aiding medical research and the search for disease genes. From owner-biophysics@net.bio.net Tue May 11 19:58:00 1999 Path: biosci!UMAIL.UMD.EDU!jf95 From: jf95@UMAIL.UMD.EDU (Jeff Forbes) Newsgroups: bionet.biophysics Subject: Postdoctoral position - Actin Polymerization - Univ. of Maryland Date: 11 May 1999 13:58:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 125 Sender: daemon@net.bio.net Distribution: world Message-ID: <4.2.0.37.19990511163400.00a5f7a0@umailsrv0.umd.edu> NNTP-Posting-Host: net.bio.net --=====================_90742653==_.ALT Content-Type: text/plain; charset="us-ascii"; format=flowed We have a postdoctoral position available after August 1, 1999 to study the polymerization of actin. If you know of any possible candidates for this position please forward this letter to them. Below is a brief description of the project. The polymerization of G-actin to F-actin occurs under particular conditions of temperature, pressure, G-actin concentration, the concentration of salts, ATP and/or ADP, etc. This polymerization from G- to F-actin is crucial to how cells hold their structure, and to how they move from place to place. The mechanism for this polymerization is not well understood. In order to understand the polymerization of actin, we need to understand the thermodynamics of the process. Our first goal is to make accurate measurements of important properties during the course of the polymerization: the mass density by dilatometry, the enthalpy by stopped flow calorimetry, the extent of polymerization by fluorescence spectrometry, and the concentration susceptibility and length scales by neutron scattering, all as functions of the several variables listed above. This information alone will give important insight into the polymerization mechanism, such as whether the volume increases or decreases, and how the heat of polymerization depends on salt concentration. Our second goal is to test the hypothesis that the polymerization of actin can be described by the same statistical mechanical models used for other second order phase transitions and other polymerizations. We will test the hypothesis by comparing the theory to the measurements discussed above. If the hypothesis holds, then (1) the mathematical framework or making calculations for actin exists and is available for use, and (2) any mechanism proposed for actin must satisfy the thermodynamics of second order phase transitions. Experience required in biochemistry and/or molecular biology of muscle or non-muscle actin. The successful candidate will also have the opportunity to interact with polymer scientists at the National Institute of Standards and Technology. The University of Maryland is an equal opportunity employer/affirmative action employer. Send CV or resume by July 1, 1999 to: Professor Sandra C. Greer or Professor Jeffrey G. Forbes University of Maryland University of Maryland Department of Chemical Engineering Department of Chemistry and Biochemistry College Park, MD 20742-2111 College Park, MD 20742-2021 sg28@umail.umd.edu jf95@umail.umd.edu http://www.ench.umd.edu/faculty/greer.html http://chem-77.umd.edu --=====================_90742653==_.ALT Content-Type: text/html; charset="us-ascii" We have a postdoctoral position available after August 1, 1999 to study the polymerization of actin. If you know of any possible candidates for this position please forward this letter to them.
Below is a brief description of the project.

The polymerization of G-actin to F-actin occurs under particular conditions of temperature, pressure, G-actin concentration, the concentration of salts, ATP and/or ADP, etc. This polymerization from G- to F-actin is crucial to how cells hold their structure, and to how they move from place to place. The mechanism for this polymerization is not well understood. In order to understand the polymerization of actin, we need to understand the thermodynamics of the process.

Our first goal is to make accurate measurements of important properties during the course of the polymerization: the mass density by dilatometry, the enthalpy by stopped flow calorimetry, the extent of polymerization by fluorescence spectrometry, and the concentration susceptibility and length scales by neutron scattering, all as functions of the several variables listed above. This information alone will give important insight into the polymerization mechanism, such as whether the volume increases or decreases, and how the heat of polymerization depends on salt concentration. 

Our second goal is to test the hypothesis that the polymerization of actin can be described by the same statistical mechanical models used for other second order phase transitions and other polymerizations. We will test the hypothesis by comparing the theory to the measurements discussed above. If the hypothesis holds, then (1) the mathematical framework or making calculations for actin exists and is available for use, and (2) any mechanism proposed for actin must satisfy the thermodynamics of second order phase transitions.

Experience required in biochemistry and/or molecular biology of muscle or non-muscle actin.
The successful candidate will also have the opportunity to interact with polymer scientists at
the National Institute of Standards and Technology. The University of Maryland is an equal opportunity employer/affirmative action employer.

Send CV or resume by July 1, 1999 to:

Professor Sandra C. Greer                       or              Professor Jeffrey G. Forbes
University of Maryland                          University of Maryland
Department of Chemical Engineering              Department of Chemistry and Biochemistry
College Park, MD 20742-2111                     College Park, MD 20742-2021
sg28@umail.umd.edu                                      jf95@umail.umd.edu
http://www.ench.umd.edu/faculty/greer.html              http://chem-77.umd.edu




 --=====================_90742653==_.ALT-- From owner-biophysics@net.bio.net Tue May 11 21:19:00 1999 From: ass@attainsuccess.com Newsgroups: bionet.biology.cardiovascular,bionet.biology.grasses,bionet.biology.n2-fixation,bionet.biology.tropical,bionet.biophysics Subject: .. white 25994 Message-ID: <12059912.0632@attainsuccess.com> Date: Wednesday, 12 May 1999 12:06:32 -0600 Organization: _+_Crew NNTP-Posting-Host: pppa12-orlandob5-4r512.saturn.bbn.com X-Trace: 11 May 1999 12:04:33 -0500, pppa12-orlandob5-4r512.saturn.bbn.com Lines: 12 Path: biosci!bcm.tmc.edu!news.tamu.edu!news.sgi.com!newsfeed.netscape.com!unlisys!news.snafu.de!news-hh.maz.net!ptdnetP!newsgate.ptd.net!newsfeed.wli.net!su-news-hub1.bbnplanet.com!news.gtei.net!news.good.net!209.54.236.18!attainsuccess.com Xref: biosci bionet.biology.cardiovascular:3122 bionet.biology.grasses:1773 bionet.biology.n2-fixation:1314 bionet.biology.tropical:3384 bionet.biophysics:4978 We supply the best sites for FREE. Use our passwords to enter the Best xxx Sites RIGHT NOW! http://pages.hotbot.com/advice/mango52/password.html http://pages.hotbot.com/advice/mango52/password.html http://pages.hotbot.com/advice/mango52/password.html See you there! Mang0! xx33E! From owner-biophysics@net.bio.net Tue May 11 21:29:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!awabi.library.ucla.edu!134.139.1.31!csulb.edu!usenet From: "Jeffrey A. Cohlberg" Newsgroups: bionet.biophysics Subject: Biochemistry lecturer position Date: Tue, 11 May 1999 14:26:47 -0700 Organization: Cal. State Univ. Long Beach Lines: 31 Message-ID: <3738A097.4CEF@csulb.edu> NNTP-Posting-Host: 134.139.180.141 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (Macintosh; I; PPC) Opening for a Full-Time Lecturer, 1999-2001 California State University, Long Beach Department of Chemistry and Biochemistry Duties: Lecturing in introductory and intermediate biochemistry courses and supervision of biochemistry laboratory courses. Opportunities may also be available for lecturing in graduate level biochemistry courses and supervision of biochemistry colloquia for senior undergraduate and MS students. Fall 1999 classes begin on August 30. Salary commensurate with qualifications and experience. Qualifications: Ph. D. in Chemistry or Biochemistry or a closely related field. Ability to communicate effectively with a diverse campus community. Prior successful teaching experience preferred. Required Documentation: Letter of application; resume; three letters of recommendation; official transcript from institution awarding highest degree. Position open until filled (or recruitment canceled). Review of applications to begin May 15. Applications and required documentation should be addressed to: Dr. N. M. Senozan, Chair, Department of Chemistry and Biochemistry, 1250 Bellflower Blvd, Long Beach, CA 90840-3903. For more information contact Dr. Nail Sezozan (nsenozan@csulb.edu) or Dr. Jeffrey Cohlberg (cohlberg@csulb.edu). Equal Opportunity/Affirmative Action/Title IX Employer From owner-biophysics@net.bio.net Tue May 11 23:25:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!news.maxwell.syr.edu!nntp2.dejanews.com!nnrp1.dejanews.com!not-for-mail From: Willy Wriggers Newsgroups: bionet.biophysics Subject: update: multi-res. docking w/ Situs 1.1 Date: Wed, 12 May 1999 00:12:41 GMT Organization: Deja.com - Share what you know. Learn what you don't. Lines: 34 Message-ID: <7hah1o$t54$1@nnrp1.deja.com> NNTP-Posting-Host: 132.239.156.46 X-Article-Creation-Date: Wed May 12 00:12:41 1999 GMT X-Http-User-Agent: Mozilla/4.51 [en] (X11; I; Linux 2.2.6 i686) X-Http-Proxy: 1.0 x35.deja.com:80 (Squid/1.1.22) for client 132.239.156.46 Hi all, A new version (1.1) of the free single-molecule, multi-resolution docking package went online at URL http://chemcca10.ucsd.edu/~situs Some of the new features are: - A more powerful and more accurate clustering algorithm to correlate features within structural data sets. - Electron microscopy: support of ASCII, CCP4, SPIDER, and MRC file formats, map file export in CCP4 format for visualization e.g. with Swiss PDB Viewer - Small-angle X-ray scattering: Support for docking of crystal structures to bead models from SAXS refinements (Chacon et al., Biophys. J. 74:2760-2775, 1998) More info can be found online and in the current issue of J. Structural Biology (1999) 125:185-195, URL http://www.academicpress.com/jsb Cheers, Willy -- Willy Wriggers, Ph.D. -- URL http://chemcca10.ucsd.edu/~wriggers Tel: (619)534-2913. Fax: (619)534-7042. E-mail: wriggers@ucsd.edu --== Sent via Deja.com http://www.deja.com/ ==-- ---Share what you know. Learn what you don't.--- From owner-biophysics@net.bio.net Wed May 12 05:44:00 1999 Path: biosci!agate!newsfeed.berkeley.edu!news-peer.gip.net!news.gsl.net!gip.net!news.idt.net!nntp2.cerf.net!socal.verio.net!nntp.ni.net!nnrp2.ni.net!not-for-mail Message-ID: <373921BD.22986EFF@ns.net> From: "J. E. Maxwell" Organization: JAYMAX Corp. X-Mailer: Mozilla 4.51 [en] (Win95; U) X-Accept-Language: en,fr,pt-BR,es,de,zh,ko,ja MIME-Version: 1.0 Newsgroups: bionet.biophysics Subject: Simulated annealing packages Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 5 Date: Wed, 12 May 1999 06:38:52 GMT NNTP-Posting-Host: 209.162.64.58 NNTP-Posting-Date: Tue, 11 May 1999 23:38:52 PDT Hello, Would like to know what software packages readers have assessed as "good" for simulated annealing of macromolecules. -Joe- From owner-biophysics@net.bio.net Wed May 12 07:41:00 1999 Path: biosci!MELBA.FKEM2.LTH.SE!fernando From: fernando@MELBA.FKEM2.LTH.SE (Fernando Luis Barroso da Silva) Newsgroups: bionet.biophysics Subject: Re: Simulated annealing packages Date: 12 May 1999 01:41:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net > Hello, > Would like to know what software packages readers have assessed as > "good" for simulated annealing of macromolecules. > -Joe- Hello, Joe. Take a look at: http://www.aic.nrl.navy.mil:80/galist/ / Fernando. -- Fernando Luis Barroso da Silva Physical Chemistry II - Chemical Center POB 124 - Lund University Fax: +46 (46) 2224543 S-221 00 Lund, Sweden Phone: +46 (46) 2228241 (lab) E-mail: fernando@melba.fkem2.lth.se +46 (46) 2220381 (office) Fernando_Luis.Barroso_da_Silva@fkem2.lth.se http://www.fkem2.lth.se/personnel/fernando/dasilva.html http://idefix.ffclrp.usp.br/barroso/fernando.html From owner-biophysics@net.bio.net Wed May 12 08:15:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!news-feed.inet.tele.dk!bofh.vszbr.cz!newsfeed.tli.de!newscore.gigabell.net!newscore.ipf.de!fu-berlin.de!news-ber1.dfn.de!news.uni-potsdam.de!newsadm From: "Frank Fürst" Newsgroups: bionet.biophysics,bionet.molbio.proteins Subject: fluorescent adenine analogues Date: Wed, 12 May 1999 11:06:45 +0200 Organization: University of Potsdam Lines: 22 Message-ID: <373944A5.3FD0D339@fuerst.de> NNTP-Posting-Host: pc207-60.biochem.uni-potsdam.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.06 [de]C-QXW0310E (Win95; I) Xref: biosci bionet.biophysics:4983 bionet.molbio.proteins:14317 Hi, I am looking for fluorescent derivatives of adenine. I want to try if I can measure adenine binding to my protein with one of these. Unfortunately, I don't even know the names of common fluorescent derivatives of adenine - but I know that there are some. Can anybody help me? Thanks in advance, Frank -- ************************************************************* Frank Fuerst, Institute of Biochemistry of Potsdam University Im Biotechnologiepark, D-14943 Luckenwalde Tel.: +49-3371-681334; Fax.: +49-3371-681339 ffrank@rz.uni-potsdam.de ************************************************************* Hi! I'm Signature Virus 99! Copy me into your signature and join the fun! From owner-biophysics@net.bio.net Wed May 12 14:32:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!nntp01.net-okbr01.exodus.net!nntp01.net-okbr01.exodus.net!198.147.221.37!news.xnet.com!news.pprd.abbott.com!not-for-mail From: Owen McCall Newsgroups: bionet.biophysics,bionet.molbio.proteins Subject: Re: fluorescent adenine analogues Date: Wed, 12 May 1999 10:24:03 -0500 Organization: Abbott Laboratories Lines: 36 Message-ID: <37399D13.AE4CDA96@intronabbott.com> References: <373944A5.3FD0D339@fuerst.de> NNTP-Posting-Host: lc942596.dhcp.pprd.abbott.com Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Trace: fizban.pprd.abbott.com 926522639 3352 10.251.241.77 (12 May 1999 15:23:59 GMT) X-Complaints-To: usenet@fizban.pprd.abott.com NNTP-Posting-Date: 12 May 1999 15:23:59 GMT X-Mailer: Mozilla 4.05 [en] (Win95; I) Xref: biosci bionet.biophysics:4984 bionet.molbio.proteins:14319 Check with Molecular Probes. They have analogs of what seems like everything. For you that would be: Molecular Probes Europe BV, PoortGebouw, Rijnsburgerweg 10, 2333 AA Leiden, The Netherlands. Phone: +31-71-5233378. They also have a great web site: www.probes.com Owen McCall Abbott Labs Frank Fürst wrote: > Hi, > > I am looking for fluorescent derivatives of adenine. I want to try if > I can measure adenine binding to my protein with one of these. > > Unfortunately, I don't even know the names of common fluorescent > derivatives of adenine - but I know that there are some. > > Can anybody help me? > > Thanks in advance, Frank > > -- > ************************************************************* > Frank Fuerst, Institute of Biochemistry of Potsdam University > Im Biotechnologiepark, D-14943 Luckenwalde > Tel.: +49-3371-681334; Fax.: +49-3371-681339 > ffrank@rz.uni-potsdam.de > ************************************************************* > Hi! I'm Signature Virus 99! Copy me into your signature and join the > fun! From owner-biophysics@net.bio.net Thu May 13 08:21:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!dispose.news.demon.net!demon!news.demon.co.uk!demon!genesys.demon.co.uk!Duncan From: "Dr. Duncan Clark" Newsgroups: bionet.biophysics,bionet.molbio.proteins Subject: Re: fluorescent adenine analogues Date: Thu, 13 May 1999 10:12:04 +0100 Organization: DNAmp Ltd. Message-ID: References: <373944A5.3FD0D339@fuerst.de> <37399D13.AE4CDA96@intronabbott.com> Reply-To: "Dr. Duncan Clark" NNTP-Posting-Host: genesys.demon.co.uk X-NNTP-Posting-Host: genesys.demon.co.uk:158.152.17.110 X-Trace: news.demon.co.uk 926586915 nnrp-11:19708 NO-IDENT genesys.demon.co.uk:158.152.17.110 X-Complaints-To: abuse@demon.net MIME-Version: 1.0 X-Newsreader: Turnpike Integrated Version 4.02 U Lines: 17 Xref: biosci bionet.biophysics:4985 bionet.molbio.proteins:14328 In article <37399D13.AE4CDA96@intronabbott.com>, Owen McCall writes >Check with Molecular Probes. Also NEN Duncan -- The problem with being on the cutting edge is that you occasionally get sliced from time to time.... Duncan Clark DNAmp Ltd. Tel: +44(0)1252376288 FAX: +44(0)8701640382 http://www.dnamp.com http://www.genesys.demon.co.uk From owner-biophysics@net.bio.net Fri May 14 07:20:00 1999 Reply-To: "Clive Delmonte" From: "Clive Delmonte" Newsgroups: bionet.biophysics Subject: DNA Structure: Puzzle #16 Date: Fri, 14 May 1999 09:14:45 +0100 Lines: 31 X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2014.211 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2014.211 NNTP-Posting-Host: 195.7.225.187 Message-ID: <373bcf28.0@news.netdirect.net.uk> X-Trace: 14 May 1999 08:22:16 GMT, 195.7.225.187 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!newsfeed.amsterdam.nl.net!sun4nl!newsfeed.tli.de!newsfeed.nacamar.de!peer.news.nildram.co.uk!news-peer.netdirect.net.uk!news.netdirect.net.uk!195.7.225.187 RNA Polymerase Slides Along DNA Kabata et al. (41) have found direct evidence that RNA polymerase slides along its target DNA. In correspondence with me, Dr Kabata indicated that he considered that there would have to be two reading frames, each deployed on alternating half turns, because every alternating half turn of the DNA would be facing away from the polymerase in a double-helical, cylindrically symmetrical duplex DNA. The true side-by-side duplex DNA found by Lee et al. under STM (Puzzle 1, ref.1) is not cylindrically symmetrical, and therefore only access from limited directions would be possible for proteins approaching the stacked base pairs in the duplex. Therefore sequence-sensitive polymerases, restriction endonucleases and transcription proteins might operate on just one face using only one reading frame to decode the base sequence. ---------------------------------------------------- 41 Visualisation of Single Molecules of RNA Polymerase Sliding along DNA; H. Kabata, O. Kurosawa, I. Arai, M. Washizu, S.A. Margarson, R.E. Glass & N. Shimamoto; SCIENCE Vol 262 (1993) 1561 - 1562 ---------------------------------------------------- Clive Delmonte E-mail: clived@ndirect.co.uk or: clivedelmonte@c-i-delmonte.freeserve.co.uk From owner-biophysics@net.bio.net Fri May 14 14:08:00 1999 Path: biosci!pravda.ucr.edu!ihnp4.ucsd.edu!newsfeed.berkeley.edu!news.maxwell.syr.edu!news.idt.net!nntp.farm.idt.net!news From: "NDRI/HBDI" Newsgroups: alt.bio.technology,alt.bio.technology.misc,bionet,bionet.biology,bionet.biophysics,bionet.cellbiol Subject: Human Tissues, Organs and DNA from Families for Medical Research Date: Fri, 14 May 1999 10:54:32 -0400 Organization: IDT (Best News In The World) Lines: 14 Message-ID: <7hhdfr$199@nnrp2.farm.idt.net> NNTP-Posting-Host: ppp-5.ts-1.ph.idt.net X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2314.1300 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2314.1300 Xref: biosci bionet.biophysics:4988 bionet.cellbiol:11799 See www.ndri.com for access to human tissues and organs for medical researchers See www.hbdi.org for information about family collections containing DNA and immortalized cells - over 600 families - for medical and genetic researchers NDRI and HBDI are non-profit organizations aiding medical research and the search for disease genes. From owner-biophysics@net.bio.net Fri May 14 19:26:00 1999 Path: biosci!candseek.com!915608 From: 915608@candseek.com Newsgroups: bionet.biophysics Subject: JOBOP RES ASSOC PROTEIN PURIFICATION Date: 14 May 1999 13:26:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 68 Sender: daemon@net.bio.net Distribution: world Message-ID: <199905142026.NAA11098@net.bio.net> NNTP-Posting-Host: net.bio.net Since your email address was listed on a related web site page or database, I thought you might help. I am seeking an individual within the following conditions: I am looking for a person to perform protein purification. The candidate will have 1+ years experience with protein purification (general chromatography skills) and characterization (SDS Page, Western Blot, slot/dot blot, HPLC, FPLC). Familiarity with UV VIS spectroscopy and computers. You will be responsible for the purification of proteins in small to medium scale from baculovirus infected cells and mammalian cells and purification and refolding of proteins from E. coli and Pichia sources. Should possess a B.S.,M.S.or MD degree in Biochemistry or medical sciences. Our client is a leading biotech firm with research facilities in New York and can provide excellent benefits (health insurance, dental, and vision plan, paid vacation and more). A high impact, high profile position with excellent opportunity for advancement. Geographic Location of Position: US-NY If you know anyone that might be interested, please forward this to them or contact: Sam Sterling DMC Voice: 609-584-8733 Ext. 218 Fax: 609-584-9575 Email: 915608@candseek.com To permanently discontinue receiving employment opportunity notices from any and all help wanted advertisers using the Candidate Seeker system, click your "Reply" button and type the word "re- move" without spaces between the letters into the SUBJECT field then click the "Send" button. Your email address will be permanently filtered from ALL future job opportunity notifications sent via the Candidate Seeker system. To temporarily filter employment opportunity notices sent via the Candidate Seeker system, type the acronym "JOBOP" into your subject filter. All employment opportunity notices sent via the Candidate Seeker system contain the acronym "JOBOP" in the subject so they may be easily filtered or blocked if so desired. Other email addresses may be permanently deleted from future contact by emailing a single blank message from the desired address to nomail@candseek.com. Enter additional addresses into the body of the message and they will also be added to the "nomail" list Please feel free to contact the candidateseeker.com feedback line at 609-584-5499. Do not use this number for job related questions. All job related questions should be directed to the employer by replying to contact addresses or phone numbers indicated at the end of the job description message. From owner-biophysics@net.bio.net Fri May 14 22:36:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!newsfeed.mathworks.com!blanket.mitre.org!news.tufts.edu!not-for-mail From: R Valluzzi Newsgroups: bionet.biophysics,sci.physics.cond-matter Subject: Cholesteric liquid crystal square grid patterns Date: Fri, 14 May 1999 19:25:44 -0400 Organization: Tufts Biotechnology Lines: 32 Message-ID: <373CB0F8.210E2CDC@marvin.tufts.edu> NNTP-Posting-Host: st7319.infonet.tufts.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: news3.tufts.edu 926724359 11278 (None) 130.64.10.53 X-Complaints-To: news@news.tufts.edu X-Mailer: Mozilla 4.5 [en] (Win98; I) X-Accept-Language: en Hello, I've been working with cholesteric peptides and have found that one of my molecules generates a pattern which is remarkable similar to the "square grid" pattern observed for cholesterics when an electric or magnetic field is applied parallel to the cholesteric axis. I haven't applied any field to this peptide, but it is in aqueous solution on a hydrophilic surface (mica) and is viscous. I think that the perpendicular component of the surface tension may be acting to mimic a field parallel to the cholesteric axis, generating the square grid pattern. Is this possible? I can find a derivation for the field strength needed and the defect type involved for both electric and magnetic fields (In Liquid Crystals by Chandrasekhar), and I could use them to derive something analogous for the surface tension. However, if this has already been done - well I can think of better ways to spend the weekend than reinventing the wheel. Has this been done? Has this texture been observed before without an electric or magnetic applied field present? Can anyone point me to some useful references? I see the pattern in dried droplets, but we've observed that the peptides preserve a cholesteric-like orientation when dried - they form cholesteric glasses. Since we have lovely detailed microscope and ESEM pictures any phenomenological references would also be appreciated. Thank you in advance for your help. RV Tufts Biotechnology Center rv@marvin.tufts.edu (no web yet) From owner-biophysics@net.bio.net Mon May 17 08:00:00 1999 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.biophysics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 May 1999 02:00:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199905170900.CAA28734@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-biophysics@net.bio.net Mon May 17 22:50:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!news.ems.psu.edu!news.cis.ohio-state.edu!nntp.service.ohio-state.edu!not-for-mail From: Alexander Showalter Newsgroups: bionet.biophysics,bionet.molbio.proteins Subject: Re: fluorescent adenine analogues Date: Mon, 17 May 1999 19:49:48 -0400 Organization: Ohio State University Lines: 58 Sender: showalter.20@showalter.mps.ohio-state.edu Message-ID: <3740AB1C.55D5CC95@osu.edu> References: <373944A5.3FD0D339@fuerst.de> NNTP-Posting-Host: showalter.mps.ohio-state.edu Mime-Version: 1.0 Content-Type: multipart/mixed; boundary="------------1912771D8D10082F70D06988" X-Trace: charm.magnus.acs.ohio-state.edu 926984715 16698 128.146.190.24 (17 May 1999 23:45:15 GMT) X-Complaints-To: usenet@charm.magnus.acs.ohio-state.edu NNTP-Posting-Date: 17 May 1999 23:45:15 GMT X-Mailer: Mozilla 4.51 [en] (WinNT; I) X-Accept-Language: en Xref: biosci bionet.biophysics:4994 bionet.molbio.proteins:14350 This is a multi-part message in MIME format. --------------1912771D8D10082F70D06988 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit A good analog for a study such as this may be 2-aminopurine, in which the extracyclic amino group is moved from C-4 to C-2. It maintains two H-bonds with thymidine, and behaves essentially the same as adenosine, except that it fluoresces quite nicely. It is typically used as part of an oligonucleotide but may also work for your purposes. "Frank Fürst" wrote: > Hi, > > I am looking for fluorescent derivatives of adenine. I want to try if > I can measure adenine binding to my protein with one of these. > > Unfortunately, I don't even know the names of common fluorescent > derivatives of adenine - but I know that there are some. > > Can anybody help me? > > Thanks in advance, Frank > > -- > ************************************************************* > Frank Fuerst, Institute of Biochemistry of Potsdam University > Im Biotechnologiepark, D-14943 Luckenwalde > Tel.: +49-3371-681334; Fax.: +49-3371-681339 > ffrank@rz.uni-potsdam.de > ************************************************************* > Hi! I'm Signature Virus 99! Copy me into your signature and join the > fun! --------------1912771D8D10082F70D06988 Content-Type: text/x-vcard; charset=us-ascii; name="showalter.20.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for Alexander Showalter Content-Disposition: attachment; filename="showalter.20.vcf" begin:vcard n:Showalter;Alexander tel;fax:(614) 292-1537 tel;work:(614) 292-6974 x-mozilla-html:FALSE org:The Ohio State University;Department of Chemistry adr:;;100 W. 18th Ave.;Columbus;OH;43210; version:2.1 email;internet:showalter.20@osu.edu fn:Alexander Showalter end:vcard --------------1912771D8D10082F70D06988-- From owner-biophysics@net.bio.net Tue May 18 11:25:00 1999 Path: biosci!COMPUSERVE.COM!13453136 From: 13453136@COMPUSERVE.COM Newsgroups: bionet.biophysics Subject: THE RULES HAVE CHANGED! Date: 18 May 1999 05:25:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 37 Sender: daemon@net.bio.net Distribution: world Message-ID: <199905181212.VAA22282@gw.naa.go.jp> NNTP-Posting-Host: net.bio.net NET-DETECTIVE-4.0 "EASY WAY TO FIND OUT ANYTHING ABOUT ANYONE" http://3465215261/3568952145232/ NET DETECTIVE 4.0 TAKES YOU BEYOND WHAT SEARCH ENGINES CAN DO! Net Detective is an amazing new tool that allows you to find EVERYTHING you ever wanted to know about your EMPLOYEES, FRIENDS, RELATIVES, SPOUSE, NEIGHBORS, even your BOSS! You can check out ANYONE, ANYTIME, ANYWHERE, right on the internet... Here's the best part: With our new INSTANT DELIVERY-SECURE ORDER SYSTEM you can have this amazing tool in your hands in less than 60 seconds and you can be doing your own on-line investigations IMMEDIATELY. To find out more about what NET DETECTIVE 4.0 can do for YOU! CLICK HERE: http://3465215261/3568952145232/ Thanks, Gayle McNeil McNeil Software Security Software Developers Since 1995 REMOVES jason9091@excite.com From owner-biophysics@net.bio.net Wed May 19 06:09:00 1999 Reply-To: "Clive Delmonte" From: "Clive Delmonte" Newsgroups: bionet.biophysics Subject: DNA Structure: Puzzle #17 (Version 2) Date: Tue, 18 May 1999 10:19:50 +0100 Lines: 55 X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2014.211 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2014.211 NNTP-Posting-Host: 195.7.227.174 Message-ID: <37425632.0@news.netdirect.net.uk> X-Trace: 19 May 1999 07:12:02 GMT, 195.7.227.174 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!news.maxwell.syr.edu!newsfeed.nacamar.de!peer.news.nildram.co.uk!news-peer.netdirect.net.uk!news.netdirect.net.uk!195.7.227.174 The Linking Number Paradox The topological geometry of double-helical DNA has been extensively explored. There remains a problem, however. Keller's electrophoretic gels (42) display quantised integer values of Tw in bands with a very clear background between bands. How would there be no blurring between bands, arising from different drag coefficients, if it were really true that the twist in a closed circular duplex DNA could take a continuum of values, through partitioning, determined by Tw=Lk - Wr ? The true side-by-side structure recorded by Lee et al. (Puzzle 1, ref. 1), for which Lk=0 in the relaxed state, would allow circular closure with only an identically integral number of positive or negative supercoils, for which Lk=Tw. The restrictive topological criteria set down by Crick et al. (43) and Bauer et al. (44), in their attempt to eliminate side-by-side models, did not apply to every side-by-side structural possibility, and do not apply to the structure found by Lee et al.: "...This proves that the linking number is not equal to zero (at least for the great majority of those (i.e., side-by-side molecules))." (43) and also their criteria apply only to such side-by-side models "...in which the two...chains do not coil around each other...but instead lie side by side over most of their length, having only a few helical turns." (44) The structure found by Lee et al. has Lk=0, identically, in the relaxed state over any and all lengths. When wound around nucleosomal cores in closed circular DNA, with -1.75 superhelical turns per nucleosome, subsequent removal of the core histones can introduce only identically -1 superhelical turn per nucleosome. Here is a straightforward, simple resolution of the Linking Number Paradox. ----------------------------------------------------------------- 42 Determination of the Number of Superhelical Turns in SV40 DNA by Gel Electrophoresis; W. Keller; Proc. Nat. Acad. Sci. Vol 72 (1975) 4876 - 4880 43 Is DNA Really a Double Helix ? F.H.C. Crick, J.C. Wang & W.R. Bauer; J Mol Biol Vo, 129 (1979) 449 - 461 44 Supercoiled DNA; W.R. Bauer, F.H.C. Crick & J.H. White; Scientific American Vol 243 (1980) 100 - 113 -------------------------------------------------------------------- Clive Delmonte E-mail: clived@ndirect.co.uk or: clivedelmonte@c-i-delmonte.freeserve.co.uk From owner-biophysics@net.bio.net Thu May 20 09:54:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!uchinews2!newsswitch.lcs.mit.edu!netnews.com!news.maxwell.syr.edu!newsfeed.nacamar.de!fu-berlin.de!server1.netnews.ja.net!pegasus.csx.cam.ac.uk!hgmp.mrc.ac.uk!biosci From: claudio@itqb.unl.pt (=?iso-8859-1?Q?Cl=E1udio?= Soares) Newsgroups: bionet.biophysics Subject: SPBf short course on simulation Date: 20 May 1999 11:48:35 +0100 Organization: MRC Human Genome Mapping Project Resource Centre Lines: 171 Message-ID: <3.0.6.32.19990520114739.0083fec0@brutus.itqb.unl.pt> NNTP-Posting-Host: mercury.hgmp.mrc.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: 8bit X-Trace: niobium.hgmp.mrc.ac.uk 927197316 18841 193.62.192.80 (20 May 1999 10:48:36 GMT) X-Complaints-To: news@hgmp.mrc.ac.uk NNTP-Posting-Date: 20 May 1999 10:48:36 GMT X-Received: from mserv1.dl.ac.uk (root@mserv1.dl.ac.uk [148.79.160.65]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id LAA05133 for ; Thu, 20 May 1999 11:48:29 +0100 (BST) X-Received: from gatekeeper.itqb.unl.pt (qmailr@gatekeeper.itqb.unl.pt [193.136.177.1]) by mserv1.dl.ac.uk with SMTP id LAA25238 (8.8.8/5.4[ref postmaster@dl.ac.uk] for dl.ac.uk from claudio@itqb.unl.pt); Thu, 20 May 1999 11:48:33 +0100 (BST) X-Precedence: first-class X-Received: (qmail 324 invoked from network); 20 May 1999 10:48:15 -0000 X-Received: from brutus.itqb.unl.pt (193.136.176.2) by gatekeeper.itqb.unl.pt with SMTP; 20 May 1999 10:48:15 -0000 X-Received: from model.itqb.unl.pt by brutus.itqb.unl.pt (5.65v4.0/1.1.8.2/30Apr98-1221PM) id AA11266; Thu, 20 May 1999 11:48:13 +0100 X-Sender: claudio@brutus.itqb.unl.pt X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) X-To: biophys@dl.ac.uk X-Cc: claudio@itqb.unl.pt X-MIME-Autoconverted: from quoted-printable to 8bit by hgmp.mrc.ac.uk id LAA05144 Dear All The Portuguese Biophysical Society (SPBf) is organising its annual short course. This year the course in entitled: SIMULATION OF BIOLOGICAL PROCESSES. PRACTICAL APPROACHES. The course is mostly aimed at Portuguese and Spanish participants, although other people are also welcomed, given that the official language is English. More information about the course follows next. Regards Cláudio Soares -------------------------------------------------------------------------- Course Information 2nd short course of the Portuguese Biophysical Society SIMULATION OF BIOLOGICAL PROCESSES. PRACTICAL APPROACHES. 2-3 October 1999, ISLA Auditorium, Santarém http://www.itqb.unl.pt/~biophysics/course.html (With online registration) Organizers: C.M.Soares (ITQB-UNL), A.M.Baptista (ITQB-UNL), M.Castanho (FCUL), M.Prieto (IST-UTL) SCOPE: The aim of this course is to give an overview of the simulation techniques used in biological sciences. The course is oriented towards people working in the areas of biochemistry, biology, chemistry and related fields; the perspective will be application-oriented rather than theoretical. Topics will cover molecular dynamics simulation (classical and mixed quantum), molecular interactions, continuum electrostatic methods, protein structure prediction, membrane organization, reaction-diffusion systems, metabolic simulation and immune system modelling. PROVISIONAL PROGRAM AND SPEAKERS Friday, 1st October Registration and welcome reception (offered by Câmara Municipal de Santarém) Saturday, 2nd October Opening remarks Manuel Prieto - Chairman of the SPBf Instituto Superior Técnico, Universidade Técnica de Lisboa, Portugal The scope of simulation techniques in biophysics Cláudio M. Soares Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Portugal Theoretical aspects of molecular mechanics/dynamics simulation. Some applications. Cláudio M. Soares Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Portugal Hydrodynamics and Brownian dynamics. Theory and applications. José Garcia de la Torre Dep. Química Física, Universidad de Murcia, Spain Inter-molecular interactions in proteins P.Nuno Palma Dep.Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Portugal Hybrid QM/MM studies of enzymatic systems Maria João Ramos Dep.Química, Faculdade de Ciências, Universidade do Porto, Portugal Continuum electrostatics in proteins: theoretical aspects and applications. António M. Baptista Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Portugal Sunday, 3rd October Protein structure prediction. Homology modelling and sidechain prediction. Joaquim Mendes Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Portugal Monte Carlo Simulation of Membrane Organization Ole G.Mouritsen Dep. Chemistry, Technical University of Denmark, Denmark A modelling and simulation approach to biological reaction-diffusion systems Joaquim Sainhas Faculdade de Motricidade Humana, Universidade Técnica de Lisboa & Instituto Gulbenkian de Ciência, Portugal Metabolic simulation Armindo Salvador Dep. Microbiology and Immunology, University of Michigan, U.S.A. Immune system modelling Jorge Carneiro Instituto Gulbenkian de Ciência, Portugal MORE INFORMATION CAN BE OBTAINED IN THE FOLLOWING WAYS: http://www.itqb.unl.pt/~biophysics/course.html (with online registration) e-mail: simul@itqb.unl.pt Tel: 351 1 4469610/613, Fax: 351 1 4433644 (Cláudio Soares, ITQB-UNL) Registrations until July 31. COURSE LOCATION: Santarém is an old town approximately 100Km north of Lisbon in the Ribatejo region of Portugal. The town is by the Tagus (Tejo) river. The course will take place at the ISLA Auditorium in the old part of Santarém, near the famous Igreja da Graça. REGISTRATION FEES: (PTE; includes the course dinner, October 2) Regular Student -------------------------------------- Non-members 12000 8000 Members SPB*/SBE** 9000 5000 Members SPBf*** 6000 2000 -------------------------------------- *Sociedade Portuguesa de Bioquímica+ **Sociedad de Biofísica de España ***Sociedade Portuguesa de Biofísica+ +You can register in the Society during the course 200.482 PTE = 1 EUR A limited number of grants will be available COURSE SPONSORS ISLA Santarém Câmara Municipal de Santarém Fundação para a Ciência e a Tecnologia Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa, TRIPOS Inc Nextvision/SGI Micromineiro -------------------------------------------------------------------------- -------------------------------------------------------------------- Cláudio Soares | Instituto de Tecnologia | Química e Biológica |Phone:(351-1)4469610/4469100 6º Piso, Av. da Republica | Fax :(351-1)4411277 Apartado 127 |email:claudio@itqb.unl.pt 2781-901 OEIRAS Portugal |http://www.itqb.unl.pt/~claudio --- From owner-biophysics@net.bio.net Thu May 20 17:24:00 1999 Path: biosci!nih.knaw.nl!A.van.Ooyen From: A.van.Ooyen@nih.knaw.nl (Arjen van Ooyen) Newsgroups: bionet.biophysics Subject: Model of Competition in Development of Nerve Connections Date: 20 May 1999 11:24:17 -0700 Organization: Netherlands Institute for Brain Research Lines: 45 Sender: daemon@net.bio.net Distribution: world Message-ID: <3744519E.4936@nih.knaw.nl> NNTP-Posting-Host: net.bio.net NEW PAPER: Competition for Neurotrophic Factor in the Development of Nerve Connections A. van Ooyen & D. J. Willshaw Proc. R. Soc. Lond. B Biol. Sci. (1999) 266: 883-892. Download full text from the following website: http://www.cns.ed.ac.uk/people/arjen/competition.html Or request a reprint of the paper version (don't forget to give your address): A.van.Ooyen@nih.knaw.nl ABSTRACT The development of nerve connections is thought to involve competition among axons for survival promoting factors, or neurotrophins, which are released by the cells that are innervated by the axons. Although the notion of competition is widely used within neurobiology, there is little understanding of the nature of the competitive process and the underlying mechanisms. We present a new theoretical model to analyse competition in the development of nerve connections. According to the model, the precise manner in which neurotrophins regulate the growth of axons, in particular the growth of the amount of neurotrophin receptor, determines what patterns of target innervation can develop. The regulation of neurotrophin receptors is also involved in the degeneration and regeneration of connections. Competition in our model can be influenced by factors dependent on and independent of neuronal electrical activity. Our results point to the need to measure directly the specific form of the regulation by neurotrophins of their receptors. -- Arjen van Ooyen, Netherlands Institute for Brain Research, Meibergdreef 33, 1105 AZ Amsterdam, The Netherlands. email: A.van.Ooyen@nih.knaw.nl website: http://www.cns.ed.ac.uk/people/arjen.html phone: +31.20.5665483 fax: +31.20.6961006 From owner-biophysics@net.bio.net Fri May 21 13:45:00 1999 Path: biosci!kfunigraz.ac.at!andreas.kungl From: andreas.kungl@kfunigraz.ac.at (andreas kungl) Newsgroups: bionet.biophysics Subject: 3rd International Conference on Molecular Structural Biology Date: 21 May 1999 07:45:46 -0700 Organization: Karl-Franzens-Universitaet Graz Lines: 326 Sender: daemon@net.bio.net Distribution: world Message-ID: <37456F88.BF1A61BD@kfunigraz.ac.at> NNTP-Posting-Host: net.bio.net The Biochemistry Subgroup of the Austrian Chemical Society is pleased to announce the Third International Conference on Molecular Structural Biology which will take place in Vienna, Austria from September 8-12, 1999 FOR DETAILED INFORMATION, PLEASE SEE BELOW OR VISIT OUR HOMEPAGE AT http://www.kfunigraz.ac.at/ipcwww/icmsb99 ************************************************************************ Introduction to the ICMSB99 The First and the Second International Conference on Molecular Structural Biology (ICMSB) took place in Vienna in September 1995 and 1997. Both conferences were very well received by all who took part, including over 250 participants from more than 20 countries worldwide. The organisers are pleased to announce the Third ICMSB, which will take place from 8th to 12th September 1999, and which will, like the previous conferences, feature internationally renowned speakers. The ICMSB99 will focus on topics covering a number of the most exciting areas in the field, with the aim of bringing together specialised scientists from different areas. It will be opened by one of the most outstanding scientists in the field of structural biology, Robert Huber, and the following four days of sessions will include Folding and Function, Novel Structures, Advances in Microscopic Methods, Structural Molecular Biology, Structure-Based Drug Design, and Prediction and Simulation. ************************************************************************ Vienna - An Attractive Conference Location The ICMSB99 will be located at the Federal Chancellery in Vienna. The city is a particularly attractive location for a conference, with its combination of historical buildings, green parks, and modern architecture. Some of the most famous city sights include Schönbrunn Palace and the Hofburg, former homes of the Habsburgs, St. Stephans Cathedral, and the colourful Hundertwasser House. Also unique to Vienna is the Prater park, with its endless green avenues and its funfair, featuring the ´´Riesenrad´´ (ferris wheel). Of course, no trip to Vienna would be complete without a visit to one of the many traditional Viennese cafés, for a piece of the famous Sachertorte. An ´´Oldtimer-Tram´´tour around the Ringstraße will take participants past many of the finest sights. ************************************************************************ Organising Committee A. Kungl, P. Andrew, A. Binder, S. Kristl, A. Schilk ************************************************************************ Scientific Committee C. Kratky, M. Sippl, A. Kungl, P. Andrew ************************************************************************ In Cooperation With Austrian Academy of Sciences Austrian Federal Chancellery ************************************************************************ Sponsored By Austrian Airlines Novartis Forschungsinstitut ************************************************************************ Scientific Programme The six sessions of the conference cover a wide range of topics and experimental methods, which will be presented in the form of plenary lectures, selected short oral communications, and posters. Wednesday 8th: Registration from 2 p.m. onwards Evening: Honorary Lecture (followed by a welcome drink and snack) Robert Huber (Max-Planck-Institute, Martinsried): Diversity and Conservation in Proteolytic Enzymes and their Inhibitors Thursday 9th: Morning Session: Advances in Microscopic Methods Werner Kühlbrandt (Max-Planck-Institute, Frankfurt): Two Conformations of Membrane Ion Pumps Hansgeorg Schindler (Linz University): Single Molecule Microscopy Methods for Structural Biology Helen Hansma (University of California, Santa Barbara): Probing Biomaterials with the Atomic Force Microscope Afternoon Session: Folding and Function Alan Fersht (Cambridge University): Minichaperones: Practical and Mechanistic Tools Thomas Kiefhaber (Biozentrum Basel): Speed Limit for Protein Folding Peter Wright (Scripps Institute): Structure and Dynamics of Unfolded Proteins and Protein Folding Intermediates Friday 10th: Morning Session: Novel Structures Michael Rossmann (Purdue University): The Assembly of Viruses Examined by Combining Crystallography and Cryo-electron Microscopy Don Wiley (Harvard University): Structural Studies of Viral Entry Mechanisms in Influenza, HIV-1 and bola Viruses Kurt Wüthrich (ETH Zürich): TROSY and BSE - Recent Progress with NMR in Structural Biology Afternoon Session: Structure-Based Drug Design Siegfried Reich (Agouron Pharmaceuticals): The Use of Protein Structural Information in Drug Design and Development: Some Examples Keith Wilson (Vertex Pharmaceuticals): Structure-Based Drug Design: Reality over Hype Poster Session I Saturday 11th: Morning Session: Structural Molecular Biology Stephen Cusack (EMBL Grenoble): tRNA and Amino Acid Recognition by Aminoacyl-tRNA Synthetases Robert Kaptein (Utrecht University): Allosteric Interactions in Protein-DNA Recognition Christoph Kratky (Graz University): Structure and Mechanism of Enzymes with a B12 Cofactor Paul Sigler (Yale University): Structure and Function in Chaperonin-Assisted Protein Folding Afternoon: Poster Session II (followed by the ´´Oldtimer-Tram´´ tour) Sunday 12th: Morning Session: Structural Genomics David Eisenberg (UCLA): Protein-Protein Interactions Terry Gaasterland (Rockefeller University): Using Patterns of Evolution Across Whole Genomes to Support Structural Genomics Target Selection John Moult (University of Maryland): The Past, Present, and Future of Protein Structure Prediction Wayne Hendrickson (Columbia University): Prospects of High-Throughput Crystallographic Structure Determination Approx. 1 p.m.: End of Conference with Farewell Drink and Snack ************************************************************************ Call for Posters Interested participants are invited to submit abstracts describing original work which has not been presented elsewhere. Abstracts should arrive no later than July 10th, 1999. Authors will be informed about the provisional acceptance of the abstract by the end of July. The presentation of the abstract as a poster will be confirmed, and included in the Book of Abstracts when one or more of the authors registers for the conference. Contributors of outstanding abstracts will be chosen by the Scientific Committee to give a 15 minute oral presentation. Poster Prize A prize of ATS 2,000 will be awarded for the best poster contribution i n terms of innovative results and presentation. ************************************************************************ Abstract Submission Camera-ready abstracts should be printed in good quality on a single (A4) sheet of paper, within the area 16 cm wide and 24 cm long. The title should be followed by the author(s) name(s), affiliation(s), and address(es). Text should be in a 12 point font with maximum 1.5 line spacing. Please send two unfolded copies of the abstract to the conference secretariat. ************************************************************************ Posters The poster boards will be 100 cm (width) x 200 cm (height). Materials for poster mounting will be provided. ************************************************************************ Conference Proceedings The lectures and poster presentations will be published by the Austrian Chemical Society in book form (with an ISBN number), and will be distributed to the participants upon arrival at the conference. Additional copies of the Conference Proceedings can be purchased for ATS 300,-. ************************************************************************ General Information Exhibition: An exhibition of instruments, accessories, software, literature, and other items is planned. Companies interested in displaying their products are kindly requested to contact the conference secretariat. Social Events: The conference will be opened by a welcome drink f ollowing the honorary lecture in the Federal Chancellery on the evening of Wednesday, September 8th. On Saturday afternoon, the conference participants will be taken on a tram tour of the city centre. Following this, all participants are encouraged to visit a typical Viennese Heurigen (wine cellar) to enjoy the local food and wine (not included in the registration fee). A further social event will be arranged for one other evening, leaving one evening free to explore Vienna. In addition, half day tours to some of Vienna´s best known sights will be organised for accompanying people (dependent on participant number). Registration (please contact the Conference Secretariat or our homepage): Registration Fee Before August 1 After August 1 Regular Participant 5.000 ATS 5.500 ATS GÖCH Member* 4.000 ATS 4.500 ATS Student** 2.500 ATS 3.000 ATS GÖCH-Students*/** 1.750 ATS 2.000 ATS Accompanying Person 500 ATS 600 ATS *Payment must be accompanied by proof of the remittance of the ´99-GÖCH membership fee **Payment must be accompanied by proof of student status The registration desk will be open from 2 p.m. on Wednesday, September 8th. The registration fee includes the Conference Proceedings and participation in all scientific sessions, the welcome and farewell drinks, lunch from Thursday to Saturday, coffee breaks, and participation in the social programme (please note: the Heurigen visit is not included). Accompanying people attend only the social programme (welcome drink, tram tour, additional evening). Accomodation is not included in the registration fee! For accomodation, to register for the Heurigen evening, and to book social events for accompanying people, please ask for the flyer of the travel agency MONDIAL CONGRESS (at the conference secretariat or at our homepage). Remittance of Fees: Remittance must be made in Austrian Schillings (ATS) payable to the Gesellschaft Österreichischer Chemiker, Arbeitsgruppe Biochemie, Account Number 0043-19265/04 at Bank Creditanstalt, Bank Code 11000. Payment is also accepted by sending a (Euro-)Cheque to the conference secretariat or by filling in the credit card form (Visa, Euro/Mastercard, Diners Club, and American Express are accepted). All charges due to bank transfer have to be paid by the sender. The sender´s name and address have to be clearly marked on every remittance. Cancellation: Applications may be cancelled up to August 1st, in which case 85% refund of fees already paid will be made. It will not be possible to offer any refunds if cancellations are made after that date. ************************************************************************ Conference Homepage Please visit our homepage at http://www.kfunigraz.ac.at/ipcwww/icmsb99 for the latest update of the programme plus further information. ************************************************************************ Key Dates July 10, deadline for submitting poster abstracts August 1, deadline for early registration fee August 1, deadline for cancellation ************************************************************************ Location Austrian Federal Chancellery (Bundeskanzleramt) Festsaal Radetzkystraße 2 A-1031 Vienna ************************************************************************ Conference Secretariat Dr. Andreas Kungl Austrian Chemical Society (GÖCH), Biochemistry Subgroupc/o Institute of Pharmaceutical Chemistry University of Graz, Universitätsplatz 1, A-8010 Graz Tel.: +43 316 380 5373, Fax: +43 316 382541 E-Mail: andreas.kungl@kfunigraz.ac.at From owner-biophysics@net.bio.net Sun May 23 10:54:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!uchinews2!newsswitch.lcs.mit.edu!newspump.monmouth.com!newspeer.monmouth.com!uunet!nyc.uu.net!lax.uu.net!newshub.csu.net!ebay.com From: "Cheap Books" Newsgroups: alt.sci.physics.new-theories,alt.sci.physics.plutonium,alt.sci.physics.spam,alt.sex.bondage.particle.physics,bionet.biophysics Subject: good science books for auction and sale Date: Sunday, 23 May 1999 04:12:40 -0600 Organization: CSUnet Lines: 4 Message-ID: <23059904.1240@ebay.com> Reply-To: "Cheap Books" NNTP-Posting-Host: nts-50.calstatela.edu Hello all, I have some good new and used science (math,chem,physic,bio) books for auction with a very cheap price at http://cgi3.ebay.com/aw-cgi/eBayISAPI.dll?ViewListedItems&userid=fistvn . Take a look if you have some time. Also check out other books I have at http://cheapbook.tsx.org with a price of $15 or less on all books(either new or used). P.S: I'm truly sorry for posting this message in this NG and if you are being annoyed by this message. From owner-biophysics@net.bio.net Mon May 24 15:46:00 1999 Path: biosci!MAILCITY.COM!YourDistance From: YourDistance@MAILCITY.COM Newsgroups: bionet.biophysics Subject: UNLIMITED Long Distance Calling for only $25/month Date: 24 May 1999 09:46:53 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 66 Sender: daemon@net.bio.net Distribution: world Message-ID: <199905241646.JAA10552@net.bio.net> Reply-To: YourDistance@bigfoot.com NNTP-Posting-Host: net.bio.net IMAGINE! UNLIMITED Long Distance Calling for only $25/month flat rate! We're in a pre launch! It was bound to happen... there is a new telephone company offering UNLIMITED US Domestic Long Distance Calling for a flat rate of $20 per month... no computer required... phone to phone... "pin drop" quality. FREE Demo and rep information available: Please call this Toll Free # 800-400-8532 Or Hit Reply and leave us your : Name & e-mail address, very Important , NO information will be sent without your e-mail Address. We use an automated system that responds only to your e-mail address. (Please use the 800 number or the Reply Button, but not both) (you will receive your requested information within 24-72 hours) Thank you! Imagine being able to make UNLIMITED Long Distance calls for only $25 a month? :::::::::::::»§«:*´`³¤³´`*:»§«::::::::::::: Making Dreams Come True! :::::::::::::»§«:*´`³¤³´`*:»§«::::::::::::: This "Hot", new, technology will also allow you to (realistically) make $2-5,000 in less than 30 days part-time! _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ If you have received this message in error, please email NoSub@bigfoot.com with the word remove in the subject. _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ From owner-biophysics@net.bio.net Tue May 25 06:52:00 1999 Path: biosci!pravda.ucr.edu!ihnp4.ucsd.edu!dog.ee.lbl.gov!newsfeed.berkeley.edu!news2.best.com!news1.best.com!news3.best.com!nntp1.ba.best.com!not-for-mail From: "www.genomejobs.com" Newsgroups: bionet.biophysics Subject: Bioinformatics Jobs at www.genomejobs.com Date: Tue, 25 May 1999 00:40:17 -0700 X-Newsreader: Microsoft Outlook Express 4.72.3155.0 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.3155.0 Lines: 27 Message-ID: <374a54eb$0$205@nntp1.ba.best.com> NNTP-Posting-Host: dynamic62.pm08.sf3d.best.com X-Trace: nntp1.ba.best.com 927618283 205 209.24.235.254 Bioinformatics Jobs at www.genomejobs.com Genome Jobs is the central resource for employment in Genomics, Bioinformatics, Biotechnology and Biocomputing. We have listings of great career opportunities in Industry and Academia to save you time and help sharpen your focus. www.genomejobs.com If you are looking for jobs in: bioinformatics, genomics, biotechnology, computers, databases, DNAChips, Microarrays, proteomics, scientists, research associates, database administrators, database architects, database engineers, software engineer, analyst programmer, scientific programmer, software developer, molecular biologist, microbiologist, cheminformatics, pharmatics, biostatistics, SAS programmer, Nanotechnology, LIMS, clinical informatics, and more. Questions? genomik@genomejobs.com -- ___________________________________________ Visit www.genomejobs.com to see more jobs with cutting edge companies in Genomics, Bioinformatics, Biotech, Bioengineering and more. From owner-biophysics@net.bio.net Tue May 25 23:55:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!news-feed.inet.tele.dk!bofh.vszbr.cz!news.belnet.be!newsfeed.wirehub.nl!surfnet.nl!rug.nl!not-for-mail From: R Valluzzi Newsgroups: bionet.biophysics,sci.phys.cond-matter,sci.physics.research Subject: Cholesteric liquid crystal square grid patterns Date: 26 May 1999 00:24:07 GMT Organization: Tufts Biotechnology Lines: 33 Approved: p.helbig@jb.man.ac.uk (sci.physics.research) Message-ID: <373C5ECD.33FA2E9B@marvin.tufts.edu> NNTP-Posting-Host: gladia.astro.rug.nl Hello, I've been working with cholesteric peptides and have found that one of my molecules generates a pattern which is remarkable similar to the "square grid" pattern observed for cholesterics when an electric or magnetic field is applied parallel to the cholesteric axis. I haven't applied any field to this peptide, but it is in aqueous solution on a hydrophilic surface (mica) and is viscous. I think that the perpendicular component of the surface tension may be acting to mimic a field parallel to the cholesteric axis, generating the square grid pattern. Is this possible? I can find a derivation for the field strength needed and the defect type involved for both electric and magnetic fields (In Liquid Crystals by Chandrasekhar), and I could use them to derive something analogous for the surface tension. However, if this has already been done - well I can think of better ways to spend the weekend than reinventing the wheel. Has this been done? Has this texture been observed before without an electric or magnetic applied field present? Can anyone point me to some useful references? I see the pattern in dried droplets, but we've observed that the peptides preserve a cholesteric-like orientation when dried - they form cholesteric glasses. Since we have lovely detailed microscope and ESEM pictures any phenomenological references would also be appreciated. Thank you in advance for your help. Dr. Regina Valluzzi Tufts Biotechnology Center rv@marvin.tufts.edu (no web yet) From owner-biophysics@net.bio.net Wed May 26 16:36:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!howland.erols.net!news-out-b.news.pipex.net.MISMATCH!tank.news.pipex.net!pipex!uunet!ams.uu.net!ffx.uu.net!hearst.acc.Virginia.EDU!murdoch.acc.Virginia.EDU!not-for-mail From: eperozo@virginia.edu Newsgroups: bionet.biophysics Subject: Post Doc: Computational Membrane Protein Structure Date: Wed, 26 May 1999 13:17:30 -0400 Organization: University of Virginia Lines: 34 Message-ID: <374C2CAA.490F@virginia.edu> NNTP-Posting-Host: spin.med.virginia.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Win95; I) Computational Membrane Protein Structure A postdoctoral position is open immediately at The University of Virginia Medical Center for a computationally oriented molecular biologist. The core project will be to develop and implement computational approaches to determine three-dimensional folds of proteins using data from site-directed spin labeling and EPR spectroscopy. The project involves the application of distance geometry methods with simulated annealing and molecular dynamics using a limited number of experimentally determined restraints (probe mobility, accessibility and inter-probe distances). The candidate will also have the freedom to pursue an experimental line of research complementing the computational studies. Requirements: - A PhD in Biophysics, Biochemistry, Chemistry, Physics or related areas. - Familiarity with distance geometry and simulated annealing methods is required. - Experience in structure determination using NMR methods is highly desirable. Applications including a CV, a description of research experience, and the addresses and telephone numbers of three references should be sent to: Dr Eduardo Perozo Department of Molecular physiology and Biological physics University of Virginia Health Science Center 1300 Jefferson Park Ave Charlottesville VA 22906 Email: eperozo@virginia.edu Ph: (804) 243-6580 Fax: (804) 982-1616 From owner-biophysics@net.bio.net Thu May 27 06:20:00 1999 Reply-To: "Clive Delmonte" From: "Clive Delmonte" Newsgroups: bionet.biophysics Subject: DNA Structure: Puzzle #19 (Version 2) Date: Thu, 27 May 1999 08:13:48 +0100 Lines: 50 X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2014.211 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2014.211 NNTP-Posting-Host: 195.7.225.21 Message-ID: <374ce485.0@news.netdirect.net.uk> X-Trace: 27 May 1999 07:21:57 GMT, 195.7.225.21 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!news-feed.inet.tele.dk!bofh.vszbr.cz!newsfeed.tli.de!newsfeed.nacamar.de!ayres.ftech.net!news.ftech.net!news.intensive.net!news.madhouse.uk.com!news-peer.netdirect.net.uk!news.netdirect.net.uk!195.7.225.21 The Diameter of Tetraplexes under AFM Vesenka and colleagues have measured the strand height of DNA tetraplexes. Their guanine-rich tetraplexes were found to have a strand height, i.e., diameter of 2.1 to 2.5nm (46). Curiously enough, this is about the height which should be recorded for the DNA double helix under AFM and STM, but no-one has ever found these values, or anything approaching them, for duplex DNA. It seems that this situation has caused Vesenka to reflect on the meaning of the height measurements for duplex and tetraplex DNA (47). Determined readers of these Puzzles will discern a different explanation (48). Two of the true side-by-side DNA duplexes caught on STM by Lee et al. (Puzzle 1, ref. 1, Figure 3b (duplex height = 1.3 nm)) are laid one upon the other, et voila! we have a true side-by-side tetraplex with a height around 2.5 nm. The implied pairing of base pairs into tetrads was described by Loewdin long ago (49). Equally important, and in accord with the structure of Lee et al., using AFM, Vesenka's group reports "...no significant differences in duplex and quadruplex DNA width." (50) ------------------------------------------------------------ 46 A new DNA nanostructure, the G-wire, imaged by scanning probe microscopy; T.C. Marsh, J. Vesenka & E. Henderson; Nucl Acids Res Vol 23 (1995) 696 - 700 47 Atomic Force Microscopy of Duplex DNA Diameter Paradox; J. Vesenka; Scanning Vol 18 (1996) 133 - 134 48 Advances in AFM & STM Applied to the Nucleic Acids; Clive Delmonte (1997); ISBN 0 9512276 2 9; LoC Number TX 4-856-037, Chapter 8, Figure 8.1 49 Electronic Aspects of Biochemistry, Chapter by P.-O. Loewdin, Editor: B. Pullman; Academic Press (1963) 50 The morphology of duplex and quadruplex DNA on mica; Tera Muir et al.; Draft paper kindly supplied by James Vesenka prior to publication in J Vac Sci & Tech. (1996) -------------------------------------------------------------- Clive Delmonte E-mail: clived@ndirect.co.uk or: clivedelmonte@c-i-delmonte.freeserve.co.uk From owner-biophysics@net.bio.net Thu May 27 11:45:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!news.maxwell.syr.edu!nntp2.dejanews.com!nnrp1.dejanews.com!not-for-mail From: josephpol@my-deja.com Newsgroups: bionet.biophysics,bionet.microbiology,bionet.neuroscience Subject: Slow Light Date: Thu, 27 May 1999 12:34:23 GMT Organization: Deja News Posting Service Lines: 14 Message-ID: <7ije4f$i90$1@nnrp1.deja.com> NNTP-Posting-Host: 146.203.100.18 X-Article-Creation-Date: Thu May 27 12:34:23 1999 GMT X-Http-User-Agent: Mozilla/4.03 [en] (Win95; I) X-Http-Proxy: 1.0 x39.deja.com:80 (Squid/1.1.22) for client 146.203.100.18 Xref: biosci bionet.biophysics:5011 bionet.microbiology:16785 bionet.neuroscience:29554 For a fascinating report on how scientists managed to slow down light to 38 miles per hour go to http://ajanta.sci.ccny.cuny.edu/~jupiter/pub/sciinfo/slowlight.html Also be sure to check out the related links such as the very clear description of Bose-Einstein condensates at http://ajanta.sci.ccny.cuny.edu/~jupiter/pub/sciinfo/boseeinstein.html Best, Joseph --== Sent via Deja.com http://www.deja.com/ ==-- ---Share what you know. Learn what you don't.--- From owner-biophysics@net.bio.net Thu May 27 22:37:00 1999 Path: biosci!agate!newsfeed.berkeley.edu!sunqbc.risq.qc.ca!cyclone.mbnet.mb.ca!typhoon.mbnet.mb.ca.POSTED!not-for-mail From: "De Groot" Newsgroups: bionet.biophysics Subject: Eyesight Continuous? Lines: 14 X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2314.1300 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2314.1300 Message-ID: <374dd600.0@goofy.BrandonU.CA> Date: Thu, 27 May 1999 23:31:44 GMT NNTP-Posting-Host: 142.13.16.203 X-Trace: typhoon.mbnet.mb.ca 927847904 142.13.16.203 (Thu, 27 May 1999 18:31:44 CDT) NNTP-Posting-Date: Thu, 27 May 1999 18:31:44 CDT Organization: MBnet Networking Inc. I hope that a) this is the place to ask such a question b) somebody can clear something up Are the images that our brain receives from our eyes continuous? It makes much more sense to imagine that the brain receives discrete signals at a very high frequency. If the latter is true, would we not see the rails on bridges or the spokes on wheels as going backwards...even in sunlight? Any clarification would be greatly appreciated. Michael De Groot degroot7@iname.com From owner-biophysics@net.bio.net Sat May 29 05:05:00 1999 Path: biosci!biosci!not-for-mail From: rick@omni.cc.purdue.edu (Rick Millane) Newsgroups: sci.edu,bionet.biophysics,bionet.xtallography,purdue.physics.general,purdue.math.general Subject: Postdoctoral positions in computational crystallography Date: 28 May 1999 23:05:25 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: <7ieumn$509@omni.cc.purdue.edu> NNTP-Posting-Host: net.bio.net Keywords: crystallography, post-doc, computational, image reconstruction Xref: biosci bionet.biophysics:5013 bionet.xtallography:4737 POSTDOCTORAL POSITIONS COMPUTATIONAL X-RAY CRYSTALLOGRAPHY POLYMER CRYSTALLOGRAPHY Purdue University Postdoctoral positions are available at Purdue University on projects concerned with the development of new computational algorithms for macromolecular and polymer crystallography. Current projects are concerned with the development of new electron density modification algorithms for protein crystallography, molecular replacement methods in fiber diffraction, and structural analysis of disordered polymer systems. Applicants should have a Ph.D. and experience in theoretical and computational methods in x-ray crystallography, image reconstrcution, or in a related field. Experience with C programming, Unix and crystallography are highly desirable. Send cv, a summary of research experience and interests, and the names of three referees to Dr. Rick Millane, Whistler Center for Carbohydrate Research, and Computational Science and Engineering Program, Purdue University, West Lafayette, IN 47907-1160, tel. 765-494-9272, fax. 765-494-7953, rmillane@purdue.edu. Purdue University is an AA/EOE. From owner-biophysics@net.bio.net Sat May 29 05:17:00 1999 Path: biosci!UQTR.UQuebec.CA!Mario_Fragata From: Mario_Fragata@UQTR.UQuebec.CA (Mário Fragata) Newsgroups: bionet.biophysics Subject: post-doctoral position Date: 28 May 1999 23:17:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 61 Sender: daemon@net.bio.net Distribution: world Message-ID: <199905290610.CAA40086@neptune.uqtr.uquebec.ca> NNTP-Posting-Host: net.bio.net Post-doctoral position for structure-function studies in the photosynthetic membrane A post-doctoral position is available for a period of one year starting September 1999 for the study of (a) the structural and functional aspects of the anionic and nonionic lipids in the thylakoid membrane of plant chloroplasts (lipid-protein interactions), and (b) the role of the thylakoid lipids on the protection of the photosynthetic membrane against the deleterious effects of temperature. In particular, we aim at elucidating how specific thylakoid lipids intervene in the function of the different protein complexes involved in the light reactions of photosynthesis. For this purpose we use (i) purified protein complexes reconstituted into lipid membranes, (ii) thylakoid membranes selectively depleted of specific thylakoid lipids by treatment with cyclodextrins, and (iii) thylakoid membranes with protein complexes selectively inactivated by heat and chemical modification, The experimental procedures include, for example, oxygen evolution and electron transfer measurements, variable fluorescence, Fourier transform infrared (FTIR) spectroscopy, polarized fluorescence, linear dichroism, and molecular modeling. For further details or application, please contact: Dr. Mário Fragata Départment de Chimie et Biologie, Section de Chimie, Université du Québec à Trois-Rivières, Trois-Rivières, Que, G9A 5H7, Canada Tel 819-376-5077, Fax 819-376-5057 E-mail fragata@uqtr.uquebec.ca -------------------------------------------------------------- PAX ~~AMAN~~MSHVIDOBA~~SHANTI~~ ~**~**~**~**~**~ Mario Fragata Univ. Quebec @ Trois-Rivieres Dept. de Chimie et Biologie, Section de Chimie Trois-Rivieres, Que, G9A 5H7, Canada voice.....819-376-5077 fax........819-376-5057 email....fragata@uqtr.uquebec.ca ~~~...~~~...~~~ "There was a most ingenious architect who had contrived a new method for building houses, by beginning at the roof and working downward to the foundation." JONATHAN SWIFT "Gulliver's Travels", Voyage to Laputa ~**~**~**~**~**~ ~~MIR~~~PAIX~~~PAZ~~~PEACE~~ From owner-biophysics@net.bio.net Sat May 29 09:02:00 1999 Path: biosci!ESYS1.ESYS.CO.UK!jiinahaa44 From: jiinahaa44@ESYS1.ESYS.CO.UK (fertviks) Newsgroups: bionet.biophysics Subject: Your.Ad.to.Millions - we.do.the.work Date: 29 May 1999 03:02:21 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 57 Sender: daemon@net.bio.net Distribution: world Message-ID: <199905291711RAA43025@eseankial.mit.edu.tw> NNTP-Posting-Host: net.bio.net ** ** EXPOSURE to YOUR MARKET ** ** Have you captured your Internet Market ? THE INTERNET There are currently over 100 million Internet users and thousands are joining each day. Currently, the Internet is experiencing both a phenomenal and exponential growth rate. Imagine the amount of prospects increasing daily. DO YOU HAVE A PRODUCT OR SERVICE ? IS YOUR MARKET BEING REACHED OR LIMITED BY: * Lack of prospects * Lack of exposure DO YOU BELIEVE YOU HAVE A VIABLE PRODUCT OR SERVICE ? Did you know that there is a method of marketing that costs pennies but have the same effect as direct postal mail? This can be made possible through recent Internet technology breakthroughs. You can now compete with the big boys, with exposure in MASSIVE NUMBERS, without expensive investments such as those associated with television commercials, radio advertising, direct postal mail, or telemarketing. THE SOLUTION - Direct E-mail Marketing Direct E-mail marketing is a proven and profitable method with a global market, incurring a small investment. It is more advantageous than conventional marketing and the risk is minimal. The prospects are millions worldwide and can be reached for much less than conventional methods. We specialize in Direct E-mail and can send your Ad to thousands of general prospects nationwide or worldwide. WE CAN SEND YOUR ADVERTISEMENT...... TO THOUSANDS.....OR.....MILLIONS - HIGHEST QUALITY SERVICE at the BEST PRICE !!! Let the Pro's help YOU !!! Like numerous businesses and organizations, we can help you GET THE WORD OUT. __________________________________________ MORE INFO (24 hrs): 800-242-0363 #1992. Or Fax 310-495-0232. __________________________________________ FOR REMOVAL, LEAVE A MESSAGE AT 800-242-0363 #2155 OR FAX 603-372-0752.Tks *-*-*-1567563495317 From owner-biophysics@net.bio.net Sat May 29 10:55:00 1999 Reply-To: "Clive Delmonte" From: "Clive Delmonte" Newsgroups: bionet.biophysics Subject: DNA Structure: Puzzle #20 (Version 2) Date: Sat, 29 May 1999 12:49:35 +0100 X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2014.211 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2014.211 NNTP-Posting-Host: 195.7.225.220 Message-ID: <374fc844.0@news.netdirect.net.uk> X-Trace: 29 May 1999 11:58:12 GMT, 195.7.225.220 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!dispose.news.demon.net!demon!colt.net!newspeer.clara.net!news.clara.net!peernews!peer.news.nildram.co.uk!news-peer.netdirect.net.uk!news.netdirect.net.uk!195.7.225.220 Lines: 39 The DNA Microprobe Experiment of Mou et al. Mou et al. (Ref.51) measured the height of duplex DNA when densely packed on cationic lipid bilayers on a mica surface. They found the height to be about 2 nm (Figure 2c). This result reminds us of that of James & Mazia (Puzzle 21, Ref.53) who also measured the film height of duplex DNA under compression in 1953 and found a value of 2.1 - 2.2 nm. Isolated duplexes, not under compression, using AFM & STM mostly have a maximum measured height of some 1.3 nm, or so, and this has led Muir et al. to suggest that "...duplex DNA may have an elliptical cross section when in contact with mica." (Puzzle 19, Ref. 50) Wilkins and co-workers had suggested that DNA had an elliptical section in 1953 (Puzzle 5, Ref.13), and the two independent determinations of the major and minor diameters by James & Mazia, namely, 2.1 - 2.2 nm x 1.2 nm, agree with this. Even in very recent work (52, page 542) the apparent height of two-dimensional sheets of DNA is reported as 1.35 - 1.45 nm using AFM. Consequently, the work of Wilkins et al., James & Mazia, Muir et al., and many others, would all suggest an elliptical cross section for dupex DNA of approximately 2.1 - 2.2 nm x 1.2 - 1.4 nm. ----------------------------------------------------------- 51 High-resolution atomic-force microscopy of DNA: the pitch of the double helix; J. Mou et al.; FEBS Lett Vol 371 (1995) 279 - 282 52 Design & self-assembly of two-dimensional DNA crystals; E. Winfree et al.; NATURE Vol 394 (1998) 539-544 ---------------------------------------------------------------- Clive Delmonte E-mail: clived@ndirect.co.uk or: clivedelmonte@c-i-delmonte.freeserve.co.uk From owner-biophysics@net.bio.net Sun May 30 09:55:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!hermes.visi.com!news-out.visi.com!hub1.ispnews.com!cyc12.deja.bcandid.com!nntp1.deja.com!nnrp2.deja.com!nntp2.deja.com!nnrp1.deja.com!not-for-mail From: klaus_frommerr@my-deja.com Newsgroups: bionet.biophysics Subject: Exact buffer calculations Date: Sun, 30 May 1999 09:59:06 GMT Organization: Deja.com - Share what you know. Learn what you don't. Lines: 40 Message-ID: <7ir25a$hku$1@nnrp1.deja.com> NNTP-Posting-Host: 62.158.15.165 X-Article-Creation-Date: Sun May 30 09:59:06 1999 GMT X-Http-User-Agent: Mozilla/4.5 [de]C-DT (Win95; U) X-Http-Proxy: 1.0 x35.deja.com:80 (Squid/1.1.22) for client 62.158.15.165 Dear scientists, Can anyone of you recommend me relevant literature and/or software programs (DOS/Win) for use with exact buffer calculations? (The software could be an Excel, Mathcad or Mathematica document too, it needs not be an external program.) At first glance, it might seem that something like this is not necessary because normal buffer calculations are very easy indeed. But: My biochemical lab work requires me to prepare buffer solutions with very exact pH values (and sometimes I have to control ionic strength as well, so titration is not the method fit best for this purpose). However, pKa values of buffers (=> pH) depend on several factors that are usually neglected, which is justified in many cases as the effects are minimal. But in my case I have to take these factors into account to be able to prepare buffers with sufficient precision for my needs. pKa values depend on 1) activity factors 2) ionic strength 3) pH 4) temperature and the normal values you can find in (bio-)chemical data collections are standard values. What I am now looking for are formulae which consider these factors and allow precise calculation of buffer solutions. Common literature in physical (bio-)chemistry did not give me the required information, at least I haven't been able to find it. I am also looking for formulae treating ampholytes and mixed buffers. I can well imagine that some of the calculations are fussy or tedious work, that was why I was also asking for software doing such calculations. I hope you can help me with my concerns. Of course, any comments are welcome as well. Thanks in advance for any advice! Best regards Klaus Frommer kf-mailbox@usa.net Sent via Deja.com http://www.deja.com/ Share what you know. Learn what you don't. From owner-biophysics@net.bio.net Sun May 30 15:57:00 1999 Path: biosci!DELTA.BC1.CA!gasooro76 From: gasooro76@DELTA.BC1.CA (buiuipe) Newsgroups: bionet.biophysics Subject: Millions want SP now... Date: 30 May 1999 09:57:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 47 Sender: daemon@net.bio.net Distribution: world Message-ID: <199905303549DAA4778@guiyopl.cz> NNTP-Posting-Host: net.bio.net (SP) COULD MAKE YOU A LOT OF MONEY! =================================== **Make Money Selling the next blockbuster software (SP)! **As far as we know, (SP) has no competition! **42 Million potential buyers on the Net! **SP's potential market is $8.4 BILLION! Even if we sell to just 1% of this huge Potential Market, this is an $84 MILLION sales target! **Your commission is 50% (DOUBLE the industry standards!) **We'll provide you with daily/weekly leads from inquiries we receive from our extensive mailings. **We'll set up your own Web page! **We give you 2 softwares to use in marketing SP ($120 value!) **Two Distributorship Packages offered: Silver and Gold! **Available Worldwide! FREE DETAILS! Call (800) 242-0363 #1992. Or Fax #: USA (310) 495-0232 OR (603) 372-0752. Mention that you need info on SP Distributorship. If you leave a fax #, we shall fax you the FREE details. After you read the Free Details, you can apply for a Silver or Gold Distributorship! Only those motivated to make serious money need apply! HURRY! Fax TODAY!! Call NOW! Limited Opportunity! - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - I am interested in receiving FREE information on SP and in possibly becoming a distributor. Please fax the FREE details ASAP! NAME:_____________________________________________ BUSINESS:_________________________________________ PHONE:___________________________________________ FAX NUMBER:_______________________________________ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TO.BE.REMOVED, CALL (800) 242-0363 #2155. Leave your email address. 23968651-21549845we634 From owner-biophysics@net.bio.net Sun May 30 17:25:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!news.u.washington.edu!dante20.u.washington.edu!dhochman From: Daryl Hochman Newsgroups: bionet.biophysics Subject: Re: Exact buffer calculations Date: Sun, 30 May 1999 11:16:26 -0700 Organization: University of Washington Lines: 64 Message-ID: References: <7ir25a$hku$1@nnrp1.deja.com> NNTP-Posting-Host: dante20.u.washington.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Trace: nntp4.u.washington.edu 928088190 35298 (None) 140.142.17.40 X-Complaints-To: help@cac.washington.edu NNTP-Posting-User: dhochman To: klaus_frommerr@my-deja.com In-Reply-To: <7ir25a$hku$1@nnrp1.deja.com> For a clear explanation of the full quantitative treatment, take a look at: P.A Stewart. How to understand acid-base: a quantitative acid-base primer for biology and medicine. Elsevier North Holland, New York. 1981. Abridged versions can be found in some of Stewart's review articles, such as: P.A. Stewart. Modern quantitative acid-base chemistry. Can. J. Physiol. 61:1444-1461. 1983 Daryl. On Sun, 30 May 1999 klaus_frommerr@my-deja.com wrote: > Dear scientists, > > Can anyone of you recommend me relevant literature and/or software > programs (DOS/Win) for use with exact buffer calculations? (The software > could be an Excel, Mathcad or Mathematica document too, it needs not be > an external program.) > At first glance, it might seem that something like this is not necessary > because normal buffer calculations are very easy indeed. But: > My biochemical lab work requires me to prepare buffer solutions with > very exact pH values (and sometimes I have to control ionic strength as > well, so titration is not the method fit best for this purpose). > However, pKa values of buffers (=> pH) depend on several factors that > are usually neglected, which is justified in many cases as the effects > are minimal. But in my case I have to take these factors into account to > be able to prepare buffers with sufficient precision for my needs. > pKa values depend on > 1) activity factors > 2) ionic strength > 3) pH > 4) temperature > and the normal values you can find in (bio-)chemical data collections > are standard values. What I am now looking for are formulae which > consider these factors and allow precise calculation of buffer > solutions. Common literature in physical (bio-)chemistry did not give me > the required information, at least I haven't been able to find it. > I am also looking for formulae treating ampholytes and mixed buffers. > I can well imagine that some of the calculations are fussy or tedious > work, that was why I was also asking for software doing such > calculations. > I hope you can help me with my concerns. Of course, any comments are > welcome as well. > Thanks in advance for any advice! > > Best regards > Klaus Frommer > kf-mailbox@usa.net > > > Sent via Deja.com http://www.deja.com/ > Share what you know. Learn what you don't. > > From owner-biophysics@net.bio.net Sun May 30 23:05:00 1999 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newspump.monmouth.com!newspeer.monmouth.com!news.maxwell.syr.edu!newsfeed.cwix.com!24.225.0.2!news.ruraltel.net!not-for-mail From: zgrihv@sssdfdf.org Newsgroups: bionet.biophysics Subject: YOURE NOT GOING TO BELIEVE THIS! 81 Date: 31 May 1999 00:09:36 GMT Organization: Rural Telephone Lines: 3 Message-ID: <7isk00$5qjv$5025@news.ruraltel.net> NNTP-Posting-Host: 98aa3b3f.ipt.aol.com X-Trace: news.ruraltel.net 928109376 191103 255.255.255.255 (31 May 1999 00:09:36 GMT) X-Complaints-To: usenet@ruraltel.net NNTP-Posting-Date: 31 May 1999 00:09:36 GMT gjljupwnbgnlz From owner-biophysics@net.bio.net Mon May 31 21:44:00 1999 Path: biosci!MARA.FI.UBA.AR!icie99 From: icie99@MARA.FI.UBA.AR (ICIE 1999) Newsgroups: bionet.biophysics Subject: ICIE'99 Date: 31 May 1999 15:44:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 95 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net CALL FOR PAPERS V International Congress on Informatic Engineering ICIE 99 August 18-20, 1999 This congress is, as the previous four ones, organized by the Department of Computing Sciences of the Faculty of Engineering of the University of Buenos Aires. The main aim of the congress is to built a forum to discuss principally the recent advances on research, industry, bussiness, gubernamental, academic and/or technological areas. Theoretical and empirical papers are particularly welcome in the following subjects: Acknowledgement based systems, Neural networks, Diffussed Systems, Artificial Intelligence, Databases, Computational Algebra, Computational Languages, Technology Dedicated to Objects, Multimedia, Vision through Computers, Robotics, Networking, Software Engineering, Operative Investigation, Distributed Systems and Networks, Real-time Systems, Education at Informatics and other areas which have to do with. Persons or investigation teams interested, have to send their complete article in definitive version by e-mail to: icie99@mara.fi.uba.ar (the article has to be sent in RTF format) or four (4) copies in spanish, portuguese or english language to: Comite de Programa ICIE'99 Departamento de Computacion Facultad de Inginieria Universidad de Buenos Aires. Paseo Colon 850. 4to. Piso (1063) Capital Federal ARGENTINA. In case of sending the article through normal post we'll thank the addition of a diskette with the correspondent file. The format will have to be the following: Arial or Times New Roman letter of 12 points, simple spacement and no more than 15 pages A4. The borders have to be 2,5 cm each side. Papers will be numbered in pencil at the rear side and at the fist page it will be indicated the application area of the article. IMPORTANT DATES Article Reception till: 10 June 1999 Acceptation Communication from: 20 June 1999 The only presentation of an article authorizes The Computer Science Department of the School of Engineering of the University of Buenos Aires to publish it at the Proceedings of the ICIE'99. However, is convenient to send an authorization with the autor's signature. Articles recieved after the established dates will not be included at the Proceedings. If you want to present your article, send an answer to icie99@mara.fi.uba.ar Program Committe Prof. Gregorio Perichinsky University of Buenos Aires Prof. Armando De Giusti University of La Plata Prof. Araceli Proto CIC - University of Buenos Aires Prof Reza Korramshagol American University Prof. Antonio Perez Ambite Politecnics University of Madrid Prof. Diana Pallioto University of Santiago del Estero Prof. Maria Feldgen University of Buenos Aires Prof. Osvaldo Clua University of Buenos Aires Prof. Gregorio Randall University of the Replublic Prof. Patricia Pesado University of La Plata Prof. Raimundo D'Aquila Technique Superior School Prof. Lic. Liliana Bibiana Rossi Technologie Institute of Buenos Aires Prof. Lic. E. Claverie Techologie Institute of Buenos Aires Prof Antonio Marsiglio University of Mar del Plata Prof. Claudio Verrastro Nuclear Energy National Commission