From owner-biophysics@net.bio.net Thu Jul 01 03:07:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!su-news-hub1.bbnplanet.com!lsanca1-snf1!news.gtei.net!news.netgate.net.nz!news.xtra.co.nz!not-for-mail From: you@somehost.somedomain (F&P Healthcare) Newsgroups: bionet.biophysics Subject: Infrared Exposure on skin cells Date: 1 Jul 1999 04:01:50 GMT Organization: Your Organization Lines: 13 Message-ID: <7lep7e$4bqu0$1@titan.xtra.co.nz> NNTP-Posting-Host: 210-55-237-136.dds.xtra.co.nz Mime-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII X-Trace: titan.xtra.co.nz 930801710 4582336 210.55.237.136 (1 Jul 1999 04:01:50 GMT) X-Complaints-To: abuse@xtra.co.nz NNTP-Posting-Date: 1 Jul 1999 04:01:50 GMT X-Newsreader: WinVN 0.99.8 (beta 1) Dear All, Is anyone able to give me an indication of the maximum IR intensity, (in W/m2), epithelial cells will tolerate before damage occours? Also, what is a generally accepted maximum temperature before damage occours? Please e-mail me: conwayr@fphcare.fp.co.nz Thanks Roger Conway New Zealand From owner-biophysics@net.bio.net Fri Jul 02 00:30:00 1999 Path: biosci!newshost.lanl.gov!logbridge.uoregon.edu!europa.netcrusader.net!209.44.33.119!hub1.ispnews.com!cyclone2.gnilink.net!typhoon2.gnilink.net.POSTED!not-for-mail From: "Douglas Nicoll" Newsgroups: alt.bio.technology,alt.bio.technology.misc,bionet,bionet.biology,bionet.biophysics,bionet.cellbiol Subject: Human Tissues, Organs, DNA - Sometimes only the real thing can give the answers you need Lines: 23 X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2314.1300 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2314.1300 Message-ID: Date: Fri, 02 Jul 1999 01:27:07 GMT NNTP-Posting-Host: 151.204.210.85 X-Trace: typhoon2.gnilink.net 930878827 151.204.210.85 (Thu, 01 Jul 1999 21:27:07 EDT) NNTP-Posting-Date: Thu, 01 Jul 1999 21:27:07 EDT Xref: biosci bionet.biophysics:5088 bionet.cellbiol:12094 See www.ndri.com for access to human tissues and organs for medical researchers See www.hbdi.org for information about family collections containing DNA and immortalized cells - over 600 families - for medical and genetic researchers NDRI - the National Disease Research Interchange and HBDI - the Human Biological Data Interchange are non-profit organizations From owner-biophysics@net.bio.net Sun Jul 04 17:39:00 1999 Path: biosci!newshost.lanl.gov!awabi.library.ucla.edu!208.134.241.18!newsfeed.cwix.com!144.212.100.101!newsfeed.mathworks.com!news-ge.switch.ch!news-fra1.dfn.de!news-lei1.dfn.de!news-nue1.dfn.de!uni-erlangen.de!news.uni-erlangen.de!not-for-mail From: "Heinrich Mörtel" Newsgroups: alt.sci.physics.new-theories,bionet.biophysics,bln.announce.tub.physik,de.sci.physik,harvard.physics.sps,ka.uni.phys,relcom.fido.mo.phystech,sci.physics,sci.physics.accelerators,sci.physics.cond-matter,sci.physics.electromag,sci.physics.particle,sci.physics.relativity,slac.physapps.cernlib,slac.seminars.physics,tnn.physics,ualberta.phys.general,ucb.physics.grads,umich.physics.general,ut.physics.general,z-netz.wissenschaft.physik,zer.z-netz.wissenschaft.physik Subject: Re: Discovery of True History of Mankind (RI-1226i) Followup-To: de.alt.flame Date: Sun, 04 Jul 1999 20:26:52 +0200 Organization: Regionales Rechenzentrum Erlangen, Germany Lines: 7 Message-ID: <377FA76C.28FD0345@rommel.stw.uni-erlangen.de> References: <377A8539.1319@AmbassadorForMankind.net> Reply-To: hmoertel@rommel.stw.uni-erlangen.de NNTP-Posting-Host: ask@frosch.rommel.stw.uni-erlangen.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.05 [de] (Win95; I) *rotfl* *rotfl* *rotfl* Also ich meine, L.R. Hubbards kriminelle Organiation namens scientology ist schon schlimm genug, aber dies hier erinnert mich dann schon eher an einen der schlechten SciFi-Romane, die Hubbard verbrochen hat... Heiner From owner-biophysics@net.bio.net Mon Jul 05 13:35:00 1999 Path: biosci!kfunigraz.ac.at!andreas.kungl From: andreas.kungl@kfunigraz.ac.at (andreas kungl) Newsgroups: bionet.biophysics Subject: 3rd International Conference on Molecular Structural Biology: Last call for Posters Date: 5 Jul 1999 07:35:26 -0700 Organization: Karl-Franzens-Universitaet Graz Lines: 330 Sender: daemon@net.bio.net Distribution: world Message-ID: <3780BFF7.8586CD55@kfunigraz.ac.at> NNTP-Posting-Host: net.bio.net The Biochemistry Subgroup of the Austrian Chemical Society is pleased to announce the Third International Conference on Molecular Structural Biology ICMSB99 which will take place in Vienna, Austria from September 8-12, 1999 FOR DETAILED INFORMATION, PLEASE SEE BELOW OR VISIT OUR HOMEPAGE AT http://www.kfunigraz.ac.at/ipcwww/icmsb99 ************************************************************************ Introduction to the ICMSB99 The First and the Second International Conference on Molecular Structural Biology (ICMSB) took place in Vienna in September 1995 and 1997. Both conferences were very well received by all who took part, including over 250 participants from more than 20 countries worldwide. The organisers are pleased to announce the Third ICMSB, which will take place from 8th to 12th September 1999, and which will, like the previous conferences, feature internationally renowned speakers. The ICMSB99 will focus on topics covering a number of the most exciting areas in the field, with the aim of bringing together specialised scientists from different areas. It will be opened by one of the most outstanding scientists in the field of structural biology, Robert Huber, and the following four days of sessions will include Folding and Function, Novel Structures, Advances in Microscopic Methods, Structural Molecular Biology, Structure-Based Drug Design, and Prediction and Simulation. ************************************************************************ Vienna - An Attractive Conference Location The ICMSB99 will be located at the Federal Chancellery in Vienna. The city is a particularly attractive location for a conference, with its combination of historical buildings, green parks, and modern architecture. Some of the most famous city sights include Schönbrunn Palace and the Hofburg, former homes of the Habsburgs, St. Stephans Cathedral, and the colourful Hundertwasser House. Also unique to Vienna is the Prater park, with its endless green avenues and its funfair, featuring the ´´Riesenrad´´ (ferris wheel). Of course, no trip to Vienna would be complete without a visit to one of the many traditional Viennese cafés, for a piece of the famous Sachertorte. An ´´Oldtimer-Tram´´tour around the Ringstraße will take participants past many of the finest sights. ************************************************************************ Organising Committee A. Kungl, P. Andrew, A. Binder, S. Kristl, A. Schilk ************************************************************************ Scientific Committee C. Kratky, M. Sippl, A. Kungl, P. Andrew ************************************************************************ In Cooperation With Austrian Academy of Sciences Austrian Federal Chancellery Austrian Federal Ministry of Science and Research ************************************************************************ Sponsored By Austrian Airlines Novartis Forschungsinstitut ************************************************************************ Scientific Programme The six sessions of the conference cover a wide range of topics and experimental methods, which will be presented in the form of plenary lectures, selected short oral communications, and posters. Wednesday 8th: Registration from 2 p.m. onwards Evening: Honorary Lecture (followed by a welcome drink and snack) Robert Huber (Max-Planck-Institute, Martinsried): Diversity and Conservation in Proteolytic Enzymes and their Inhibitors Thursday 9th: Morning Session: Advances in Microscopic Methods Werner Kühlbrandt (Max-Planck-Institute, Frankfurt): Two Conformations of Membrane Ion Pumps Hansgeorg Schindler (Linz University): Single Molecule Microscopy Methods for Structural Biology Helen Hansma (University of California, Santa Barbara): Probing Biomaterials with the Atomic Force Microscope Afternoon Session: Folding and Function Alan Fersht (Cambridge University): Minichaperones: Practical and Mechanistic Tools Thomas Kiefhaber (Biozentrum Basel): Speed Limit for Protein Folding Peter Wright (Scripps Institute): Structure and Dynamics of Unfolded Proteins and Protein Folding Intermediates Friday 10th: Morning Session: Novel Structures Michael Rossmann (Purdue University): The Assembly of Viruses Examined by Combining Crystallography and Cryo-electron Microscopy Don Wiley (Harvard University): Structural Studies of Viral Entry Mechanisms in Influenza, HIV-1 and bola Viruses Kurt Wüthrich (ETH Zürich): TROSY and BSE - Recent Progress with NMR in Structural Biology Afternoon Session: Structure-Based Drug Design Siegfried Reich (Agouron Pharmaceuticals): The Use of Protein Structural Information in Drug Design and Development: Some Examples Keith Wilson (Vertex Pharmaceuticals): Structure-Based Drug Design: Reality over Hype Poster Session I Saturday 11th: Morning Session: Structural Molecular Biology Stephen Cusack (EMBL Grenoble): tRNA and Amino Acid Recognition by Aminoacyl-tRNA Synthetases Robert Kaptein (Utrecht University): Allosteric Interactions in Protein-DNA Recognition Christoph Kratky (Graz University): Structure and Mechanism of Enzymes with a B12 Cofactor Paul Sigler (Yale University): Structure and Function in Chaperonin-Assisted Protein Folding Afternoon: Poster Session II (followed by the ´´Oldtimer-Tram´´ tour) Sunday 12th: Morning Session: Structural Genomics David Eisenberg (UCLA): Protein-Protein Interactions Terry Gaasterland (Rockefeller University): Using Patterns of Evolution Across Whole Genomes to Support Structural Genomics Target Selection John Moult (University of Maryland): The Past, Present, and Future of Protein Structure Prediction Wayne Hendrickson (Columbia University): Prospects of High-Throughput Crystallographic Structure Determination Approx. 1 p.m.: End of Conference with Farewell Drink and Snack ************************************************************************ Call for Posters Interested participants are invited to submit abstracts describing original work which has not been presented elsewhere. Abstracts should arrive no later than July 10th, 1999. Authors will be informed about the provisional acceptance of the abstract by the end of July. The presentation of the abstract as a poster will be confirmed, and included in the Book of Abstracts when one or more of the authors registers for the conference. Contributors of outstanding abstracts will be chosen by the Scientific Committee to give a 15 minute oral presentation. Poster Prize A prize of ATS 2,000 will be awarded for the best poster contribution in terms of innovative results and presentation. ************************************************************************ Abstract Submission Camera-ready abstracts should be printed in good quality on a single (A4) sheet of paper, within the area 16 cm wide and 24 cm long. The title should be followed by the author(s) name(s), affiliation(s), and address(es). Text should be in a 12 point font with maximum 1.5 line spacing. Please send two unfolded copies of the abstract to the conference secretariat. ************************************************************************ Posters The poster boards will be 100 cm (width) x 200 cm (height). Materials for poster mounting will be provided. ************************************************************************ Conference Proceedings The lectures and poster presentations will be published by the Austrian Chemical Society in book form (with an ISBN number), and will be distributed to the participants upon arrival at the conference. Additional copies of the Conference Proceedings can be purchased for ATS 300,-. ************************************************************************ General Information Exhibition: An exhibition of instruments, accessories, software, literature, and other items is planned. Companies interested in displaying their products are kindly requested to contact the conference secretariat. Social Events: The conference will be opened by a welcome drink following the honorary lecture in the Federal Chancellery on the evening of Wednesday, September 8th. On Saturday afternoon, the conference participants will be taken on a tram tour of the city centre. Following this, all participants are encouraged to visit a typical Viennese Heurigen (wine cellar) to enjoy the local food and wine (not included in the registration fee). A further social event will be arranged for one other evening, leaving one evening free to explore Vienna. In addition, half day tours to some of Vienna´s best known sights will be organised for accompanying people (dependent on participant number). Registration (please contact the Conference Secretariat or our homepage): Registration Fee Before August 1 After August 1 Regular Participant 5.000 ATS 5.500 ATS GÖCH Member* 4.000 ATS 4.500 ATS Student** 2.500 ATS 3.000 ATS GÖCH-Students*/** 1.750 ATS 2.000 ATS Accompanying Person 500 ATS 600 ATS *Payment must be accompanied by proof of the remittance of the ´99-GÖCH membership fee **Payment must be accompanied by proof of student status The registration desk will be open from 2 p.m. on Wednesday, September 8th. The registration fee includes the Conference Proceedings and participation in all scientific sessions, the welcome and farewell drinks, lunch from Thursday to Saturday, coffee breaks, and participation in the social programme (please note: the Heurigen visit is not included). Accompanying people attend only the social programme (welcome drink, tram tour, additional evening). Accomodation is not included in the registration fee! For accomodation, to register for the Heurigen evening, and to book social events for accompanying people, please ask for the flyer of the travel agency MONDIAL CONGRESS (at the conference secretariat or at our homepage). Remittance of Fees: Remittance must be made in Austrian Schillings (ATS) payable to the Gesellschaft Österreichischer Chemiker, Arbeitsgruppe Biochemie, Account Number 0043-19265/04 at Bank Creditanstalt, Bank Code 11000. Payment is also accepted by sending a (Euro-)Cheque to the conference secretariat or by filling in the credit card form (Visa, Euro/Mastercard, Diners Club, and American Express are accepted). All charges due to bank transfer have to be paid by the sender. The sender´s name and address have to be clearly marked on every remittance. Cancellation: Applications may be cancelled up to August 1st, in which case 85% refund of fees already paid will be made. It will not be possible to offer any refunds if cancellations are made after that date. ************************************************************************ Conference Homepage Please visit our homepage at http://www.kfunigraz.ac.at/ipcwww/icmsb99 for the latest update of the programme plus further information. ************************************************************************ Key Dates July 10, deadline for submitting poster abstracts August 1, deadline for early registration fee August 1, deadline for cancellation ************************************************************************ Location Austrian Federal Chancellery (Bundeskanzleramt) Festsaal Radetzkystraße 2 A-1031 Vienna ************************************************************************ Conference Secretariat Dr. Andreas Kungl Austrian Chemical Society (GÖCH), Biochemistry Subgroupc/o Institute of Pharmaceutical Chemistry University of Graz, Universitätsplatz 1, A-8010 Graz Tel.: +43 316 380 5373, Fax: +43 316 382541 E-Mail: andreas.kungl@kfunigraz.ac.at From owner-biophysics@net.bio.net Mon Jul 05 16:54:00 1999 Path: biosci!KINGFOO.NET!design From: design@KINGFOO.NET Newsgroups: bionet.biophysics Subject: E-Commerce For The New Millenium Date: 5 Jul 1999 10:54:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 53 Sender: daemon@net.bio.net Distribution: world Message-ID: <199907051754.MAA25134@elite.oneandall.net> NNTP-Posting-Host: net.bio.net Is your business currently on the Internet? 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This is a NEWSMASTER Direct mailing List! *** THIS IS RESPONSIBLE COMMERCIAL E-MAIL *** This message complies with the United States Federal Requirements for commercial E-mail (UCE). Message is sent in compliance of the new e-mail bill: SECTION 301. Paragraph (a)(2)(C) of S. 1618 TITLE III passed by 105th U.S. Congress this letter can not be considered Spam as long as we include: Contact information & a Remove Link Reply to this e-mail with remove in the subject line to be deleted. ------------------------------------------------------------------------------------------------------------------- From owner-biophysics@net.bio.net Mon Jul 05 18:43:00 1999 Path: biosci!newshost.lanl.gov!logbridge.uoregon.edu!dispose.news.demon.net!demon!colt.net!newspeer.clara.net!news.clara.net!remarQ-uK!remarQ.com!supernews.com!tank.news.pipex.net!pipex!bore.news.pipex.net!not-for-mail From: "Robert Parker" Newsgroups: alt.sci.physics,bionet.biophysics Subject: quantitative effect of human body on EM waves! Date: Mon, 5 Jul 1999 20:32:51 +0100 Organization: UUNET WorldCom server (post doesn't reflect views of UUNET WorldCom Message-ID: <7lr1if$lpa$1@lure.pipex.net> NNTP-Posting-Host: userm265.uk.uudial.com X-Trace: lure.pipex.net 931203471 22314 193.149.78.61 (5 Jul 1999 19:37:51 GMT) X-Complaints-To: abuse@uk.uu.net NNTP-Posting-Date: 5 Jul 1999 19:37:51 GMT X-Newsreader: Microsoft Outlook Express 4.72.2106.4 X-Mimeole: Produced By Microsoft MimeOLE V4.72.2106.4 Lines: 20 Yes, that's right, I'm looking for the effect of the human body on EM waves!!! I've looked everywhere, but every book/thesis I can find has no values for permittivity/speed of light/attenuation of a wave in the human body. I hope eventually to build a system to sense the position of parts of the body in 3D space to a very high accuracy. To do this, due to the limits of electronic equipment, timings are not feasible. I suspect that at some frequencies EM waves will be unattenuated by the human body (to be useful I need less than 0.5% attenuation). If anyone could point me towards a journal or book with this kind of information in it, I would be very grateful. Yours, Richard Ollerenshaw From owner-biophysics@net.bio.net Mon Jul 05 22:05:00 1999 Path: biosci!VENUS.LIKOM.COM.MY!nee75 From: nee75@VENUS.LIKOM.COM.MY (lodemtc) Newsgroups: bionet.biophysics Subject: Reaching.Global.-Exposure, the internet power Date: 5 Jul 1999 16:05:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 42 Sender: daemon@net.bio.net Distribution: world Message-ID: <19990705811VAA21527@numcerz.iimahd.ernet.in> NNTP-Posting-Host: net.bio.net IS YOUR MARKET BEING REACHED OR LIMITED BY: * Geographic boundaries or location * lack of NEW leads * lack of NEW exposure DO YOU BELIEVE YOU HAVE A VIABLE PRODUCT OR SERVICE ? 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ACT.NOW --> reach.your.untapped.internet.market ! _-----_-----_-----_-----_-----_-----_ Send a FAX to : 781,240,7780 Send your: 1) name 2) email 3) tel # 19hiu*niu5 184syt52 From owner-biophysics@net.bio.net Wed Jul 07 12:40:00 1999 Path: biosci!newshost.lanl.gov!logbridge.uoregon.edu!news.maxwell.syr.edu!news.idt.net!nntp.farm.idt.net!news From: "NDRI/HBDI" Newsgroups: alt.bio.technology,alt.bio.technology.misc,bionet,bionet.biology,bionet.biophysics,bionet.cellbiol Subject: Autopsy, Surgical Discard and Transplantation Tissues for Now Available for Research Date: Wed, 7 Jul 1999 09:27:10 -0400 Organization: IDT (Best News In The World) Lines: 16 Message-ID: <7lvkro$8b5@nnrp1.farm.idt.net> NNTP-Posting-Host: ppp-18.ts-1.ph.idt.net X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2314.1300 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2314.1300 Xref: biosci bionet.biophysics:5094 bionet.cellbiol:12115 See www.ndri.com for access to human tissues and organs for medical researchers See www.hbdi.org for information about family collections containing DNA and immortalized cells - over 600 families - for medical and genetic researchers NDRI and HBDI are non-profit organizations aiding medical research and the search for disease genes. From owner-biophysics@net.bio.net Wed Jul 07 19:59:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!nntp.cs.ubc.ca!sunqbc.risq.qc.ca!carnaval.risq.qc.ca.POSTED!not-for-mail From: asheft@po-box.mcgill.ca (Alex Sheftel) Newsgroups: bionet.biophysics Subject: Western from thin IEF gel. Message-ID: <3785bf33.25537401@news.mcgill.ca> X-Newsreader: Forte Agent 1.5/32.452 MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 12 Date: Wed, 07 Jul 1999 20:53:27 GMT NNTP-Posting-Host: 206.167.227.2 X-Complaints-To: abuse@mcgill.ca X-Trace: carnaval.risq.qc.ca 931380807 206.167.227.2 (Wed, 07 Jul 1999 16:53:27 EDT) NNTP-Posting-Date: Wed, 07 Jul 1999 16:53:27 EDT Hi, there! I am trying to do western blot after separating protein by thin gel IEF(0.4mm). I would like to know how to transfer protein from IEF gel to nitrocellulose membrane. I found one method that "Netfix has to be used with Gelbond PAG film to strip gel out of the PAG film" It seems to be available from the company named Serva (Heidelburg, Germany). However, I could not locate this company. Does anyone know what Netfix is and where to get this thing? Or does anyone has any other method for western from IEF gel? Thank you very much. From owner-biophysics@net.bio.net Thu Jul 08 11:33:00 1999 Path: biosci!nih.knaw.nl!A.van.Ooyen From: A.van.Ooyen@nih.knaw.nl (Arjen van Ooyen) Newsgroups: bionet.biophysics Subject: Competition for Neurotrophic Factor Date: 8 Jul 1999 05:33:19 -0700 Organization: Netherlands Institute for Brain Research Lines: 47 Sender: daemon@net.bio.net Distribution: world Message-ID: <378498F2.7B22@nih.knaw.nl> NNTP-Posting-Host: net.bio.net NEW PAPER Competition for Neurotrophic Factor in the Development of Nerve Connections A. van Ooyen & D. J. Willshaw Proc. R. Soc. Lond. B Biol. Sci. (1999) 266: 883-892 Request reprint: A.van.Ooyen@nih.knaw.nl Or download from: http://www.cns.ed.ac.uk/people/arjen/competition.html ----------------------------------------------------------- ABSTRACT The development of nerve connections is thought to involve competition among axons for survival promoting factors, or neurotrophins, that are released by the targets they innervate. Although the notion of competition is widely used within neurobiology, there is little understanding of the nature of the competitive process and the underlying mechanisms. We present a new theoretical model to analyse competition in the development of nerve connections. According to the model, the precise manner in which neurotrophins regulate the growth of axons, in particular the growth of their amount of neurotrophin receptor, determines what patterns of target innervation can develop. The regulation of neurotrophin receptors is involved also in the degeneration and regeneration of connections. Competition in our model can be influenced by factors dependent on and independent of neuronal electrical activity. Our results point to the need to measure directly the specific form of the regulation by neurotrophins of their receptors. -- Arjen van Ooyen, Netherlands Institute for Brain Research, Meibergdreef 33, 1105 AZ Amsterdam, The Netherlands. email: A.van.Ooyen@nih.knaw.nl website: http://www.cns.ed.ac.uk/people/arjen.html phone: +31.20.5665483 fax: +31.20.6961006 From owner-biophysics@net.bio.net Wed Jul 14 11:38:00 1999 Message-ID: <378C8358.3DB37168@pyl.unibe.ch> Date: Wed, 14 Jul 1999 14:32:32 +0200 From: Ernst Niggli Reply-To: niggli@pyl.unibe.ch Organization: University of Bern, Switzerland X-Mailer: Mozilla 4.61 (Macintosh; I; PPC) X-Accept-Language: en MIME-Version: 1.0 Newsgroups: bionet.biophysics Subject: Post doc positions Content-Type: multipart/mixed; boundary="------------1E08B2715555225CC4199A5F" NNTP-Posting-Host: pylma9.unibe.ch X-Trace: 14 Jul 1999 14:32:23 +0100, pylma9.unibe.ch Path: biosci!agate!newsfeed.berkeley.edu!dispose.news.demon.net!demon!colt.net!news0.de.colt.net!blackbush.xlink.net!news-ge.switch.ch!news-zh.switch.ch!news.unibe.ch!pylma9.unibe.ch Lines: 46 This is a multi-part message in MIME format. --------------1E08B2715555225CC4199A5F Content-Type: text/plain; charset=us-ascii; x-mac-type="54455854"; x-mac-creator="4D4F5353" Content-Transfer-Encoding: 7bit We have two positions available on the Post-Doc level in a project investigating the biophysics of cardiac muscle. For additional information see: http://beam.to/calcium_quark With best regards Ernst Niggli Department of Physiology University of Bern Bern Switzerland --------------1E08B2715555225CC4199A5F Content-Type: text/x-vcard; charset=us-ascii; name="niggli.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for Ernst Niggli Content-Disposition: attachment; filename="niggli.vcf" begin:vcard n:Niggli;Ernst tel;fax:+41 31 6314611 tel;work:+41 31 631 8717/30 (Lab/Office) x-mozilla-html:FALSE url:http://pylwww.unibe.ch/~niggli/ org:University of Bern;Dept. Physiology adr:;;Bühlplatz 5;Bern;;3012;Switzerland version:2.1 x-mozilla-cpt:;3 fn:Ernst Niggli end:vcard --------------1E08B2715555225CC4199A5F-- From owner-biophysics@net.bio.net Wed Jul 14 12:10:00 1999 Path: biosci!newshost.lanl.gov!logbridge.uoregon.edu!howland.erols.net!news.idt.net!nntp.farm.idt.net!news From: "NDRI/HBDI" Newsgroups: alt.bio.technology,alt.bio.technology.misc,bionet,bionet.biology,bionet.biophysics,bionet.cellbiol Subject: Human Tissues, Organs and DNA from Families for Medical Research Date: Wed, 14 Jul 1999 08:31:12 -0400 Organization: IDT (Best News In The World) Lines: 14 Message-ID: <7mi07a$ac5@nnrp4.farm.idt.net> NNTP-Posting-Host: ppp-19.ts-1.ph.idt.net X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2314.1300 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2314.1300 Xref: biosci bionet.biophysics:5104 bionet.cellbiol:12146 See www.ndri.com for access to human tissues and organs for medical researchers See www.hbdi.org for information about family collections containing DNA and immortalized cells - over 600 families - for medical and genetic researchers NDRI and HBDI are non-profit organizations aiding medical research and the search for disease genes. From owner-biophysics@net.bio.net Wed Jul 14 15:36:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!arclight.uoregon.edu!wn4feed!wn3feed!worldnet.att.net!208.134.241.18!newsfeed.cwix.com!128.230.129.106!news.maxwell.syr.edu!nntp2.deja.com!nnrp1.deja.com!not-for-mail From: irpeterson@my-deja.com Newsgroups: bionet.biophysics Subject: Re: Western from thin IEF gel. Date: Wed, 14 Jul 1999 16:15:16 GMT Organization: Deja.com - Share what you know. Learn what you don't. Lines: 36 Message-ID: <7mid27$kfn$1@nnrp1.deja.com> References: <3785bf33.25537401@news.mcgill.ca> NNTP-Posting-Host: 194.66.39.67 X-Article-Creation-Date: Wed Jul 14 16:15:16 1999 GMT X-Http-User-Agent: Mozilla/4.51 [en] (Win95; I) X-Http-Proxy: 1.0 x23.deja.com:80 (Squid/1.1.22) for client 194.66.39.67 In article <3785bf33.25537401@news.mcgill.ca>, asheft@po-box.mcgill.ca (Alex Sheftel) wrote: > Hi, there! > > I am trying to do western blot after separating protein by thin gel > IEF(0.4mm). I would like to know how to transfer protein from IEF gel > to nitrocellulose membrane. I found one method that "Netfix has to be > used with Gelbond PAG film to strip gel out of the PAG film" It seems > to be available from the company named Serva (Heidelburg, Germany). > However, I could not locate this company. Does anyone know what > Netfix is and where to get this thing? Or does anyone has any other > method for western from IEF gel? > > Thank you very much. > Dear Alex, I've been trying for a few days to post you this, and have just found that the software doesn't like web addresses (full URLs) being included in the text of a message. I know why you couldn't find Serva - it's because they're in Heidelberg, not Heidelburg. The address is in the German phonebook (on the web) and is: Serva Electrophoresis GmbH 69115 Heidelberg, Carl-Benz-Str. 7 Tel: +49/ 62 21/ 1 38 40-0 Cheers, Ian. Sent via Deja.com http://www.deja.com/ Share what you know. Learn what you don't. From owner-biophysics@net.bio.net Thu Jul 15 00:09:00 1999 Path: biosci!CHEM.UFL.EDU!tan From: tan@CHEM.UFL.EDU (Weihong Tan) Newsgroups: bionet.biophysics Subject: Research Associate positions at University of Florid Date: 14 Jul 1999 18:09:34 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 37 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Research associate positions at the Department of Chemistry and UF Brain Institute, University of Florida Bioanalytical Chemistry and Biomedical Engineering Applications are invited for one or two postdoctoral positions at the Department of Chemistry and at the UF Brain Institute, University of Florida. The successful applicant should have a strong background in ANY ONE of the following areas: Bioanalytical Chemistry; Biophysics; Biomedical Engineering; Neuroscience. Experiences in any one of the following fields are highly desired: biochemical sensor development, optical imaging of biological samples, scanning probe microscopy, or DNA/RNA/protein studies. We are carrying out exciting research work in bioanalytical chemistry, biomedical engineering and biophysics. These research activities are funded by the US National Science, The Whitaker Foundation for Biomedical Engineering, the Office of Naval Research, Department of Defense and The Arnold Beckman Foundation and by industrial companies. Our group has labs both in the Chemistry Department and in the newly established UF Brain Institute. This Institute is a national center for neuroscience and brain research. At the University of Florida we have excellent working and living environment. The weather at Gainesville is excellent, and there are many outdoor activities and cultural events throughout the year. Interested applicants should make an initial contact with Prof. Tan through e-mail, or send a CV to the following address: Prof. Weihong Tan Department of Chemistry and The UF Brain Institute University of Florida, Gainesville, FL 32611-7200 e-mail: tan@chem.ufl.edu; Tel: 352-846-2410; Fax: 352-392-4651 Web page: http://www.chem.ufl.edu/Groups/Tan/ From owner-biophysics@net.bio.net Sat Jul 17 04:17:00 1999 Path: biosci!rutgers!gatech!howland.erols.net!news.maxwell.syr.edu!news1.best.com!news3.best.com!nntp1.ba.best.com!not-for-mail Reply-To: "www.genomejobs.com" From: "www.genomejobs.com" Newsgroups: bionet.biophysics Subject: List of Bioinformatics jobs available listed at www.genomejobs.com 7/16/99 Date: Fri, 16 Jul 1999 21:51:43 -0700 X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2314.1300 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2314.1300 Lines: 202 Message-ID: <37900d9b$0$210@nntp1.ba.best.com> NNTP-Posting-Host: dynamic38.pm07.sf3d.best.com X-Trace: nntp1.ba.best.com 932187547 210 209.24.235.166 List of Bioinformatics jobs available listed at www.genomejobs.com 7/16/99 We advertise jobs in Genomics, Bioinformatics, Biotech, and other cutting edge life science areas where biology meets computers and micro-robotics. If this page does not load, just go to www.genomejobs.com/jobs.htm These are GREAT OPPORTUNITIES with GREAT COMPANIES!!!!!! _________________________________________________ 3rd Millennium Cambridge, MA http://www.genomejobs.com/jobs/3rd.htm Database Project Manager Web Applications Developer Bioinformatics Manager Affymetrix Santa Clara, CA http://www.genomejobs.com/jobs/affymetrix.htm Senior Scientist/Manager Staff Scientist Albert Einstein College of Medicine Bronx, NY http://www.genomejobs.com/jobs/einstein.htm Bioinformatics Associate American Cyanamid Princeton, NJ http://www.genomejobs.com/jobs/ac.htm Biologist - Biotechnology Ariad Pharmaceuticals, Cambridge MA http://www.genomejobs.com/jobs/ariad.htm Senior Scientist - Gene Expression Informatics Bioinformatics Scientist Senior Bioinformatics Scientist/Group Leader Axys Pharmaceuticals S. 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Advertise your jobs with us at www.genomejobs.com You are receiving this Email because you are involved in the biotechnology or similar fields. If you do not wish to receive this, please reply with remove in the suject. -- ___________________________________________ Visit www.genomejobs.com to see more jobs with cutting edge companies in Genomics, Bioinformatics, Biotech, Bioengineering and more. -- ___________________________________________ Visit www.genomejobs.com to see more jobs with cutting edge companies in Genomics, Bioinformatics, Biotech, Bioengineering and more. From owner-biophysics@net.bio.net Sat Jul 17 08:00:00 1999 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.biophysics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Jul 1999 02:00:11 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199907170900.CAA29416@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-biophysics@net.bio.net Tue Jul 20 21:01:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!newsfeed.acns.nwu.edu!news.cc.uic.edu!not-for-mail From: Sangkyung Kim Newsgroups: bionet.immunology,sci.bio.technology,bionet.biophysics Subject: What is the most effective enzyme-Turnover No. Date: Tue, 20 Jul 1999 16:37:43 -0500 Organization: University of Illinois at Chicago Lines: 12 Distribution: inet Message-ID: <3794EC27.168ABB28@uic.edu> NNTP-Posting-Host: rumenka.eecs.uic.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.51 [en] (WinNT; I) X-Accept-Language: en,ko Xref: biosci bionet.immunology:16440 bionet.biophysics:5111 Hi guys! I looking for the turn over number of Salicylate Hydroxylase. If you know good reference for enzyme's kinetic information, share with me please. -- Sangkyung Kim - skim62@uic.edu University of Illinois at Chicago Micro-Actuator Laboratory Department of Bioengineering From owner-biophysics@net.bio.net Wed Jul 21 07:50:00 1999 Path: biosci!agate!newsfeed.berkeley.edu!nntp.abs.net!newsfeed.fast.net!uunet!ffx.uu.net!news1-gui.server.ntli.net!news5-gui.server.ntli.net!ntli.net!server4.netnews.ja.net!server2.netnews.ja.net!hgmp.mrc.ac.uk!pegasus.csx.cam.ac.uk!not-for-mail From: Yu Wai Chen Newsgroups: bionet.biophysics Subject: static light scattering and M.W. measurement Date: Wed, 21 Jul 1999 09:44:14 +0100 Organization: MRC Centre for Protein Engineering Lines: 29 Message-ID: <3795885E.7C03ACD6@mrc-lmb.cam.ac.uk> NNTP-Posting-Host: ind4.mrc-lmb.cam.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.6C-SGI [en] (X11; I; IRIX64 6.4 IP30) X-Accept-Language: zh-TW, zh-CN, en Dear all, It seems that Protein Solution's discussion forum is not a very active one and questions posted there simply get no answer. I'll try this one. I tried to measure accurate M.W. of my protein using static light scattering so I made light scattering measurements with sample in various concentration and do the plot. From this total intensity light scattering experiment I obtained a value which is quite close to the calculated M.W. of my protein. However, I know that my protein exists as dimer in the condition I used for these experiments. From dynamic light scattering experiments of the same samples, I obtained M.W. corresponding to a dimer. So DLS gives me M.W. that is two times the M.W. obtained from SLS. My question is, does the SLS method always give monomeric M.W.? Are my results correct? In concentration calculations in the SLS plot, I used the monomeric M.W. Thanks a lot. -- =================================================================== Yu Wai CHEN, Ph.D. .................. email:ywc@mrc-lmb.cam.ac.uk Centre for Protein Engineering, tel:+44-(0)1223-402148 MRC Centre, Hills Rd, Cambridge CB2 2QH, UK fax:+44-(0)1223-402140 WWW homepage: http://www.mrc-cpe.cam.ac.uk/~ywc From owner-biophysics@net.bio.net Wed Jul 21 19:24:00 1999 Path: biosci!candseek.com!915608 From: 915608@candseek.com Newsgroups: bionet.biophysics Subject: JOBOP RESEARCH ASSOCIATE PROTEIN PURIFICATION Date: 21 Jul 1999 13:24:19 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 69 Sender: daemon@net.bio.net Distribution: world Message-ID: <199907212024.NAA25465@net.bio.net> NNTP-Posting-Host: net.bio.net Since your email address was listed on a related web site page or database, I thought you might help. I am seeking an individual within the following conditions: a person to perform protein purification. The candidate will have 1+ years experience with protein purification (general chromatography skills) and characterization (SDS Page, Western Blot, slot/dot blot, HPLC). Familiarity with UV VIS spectroscopy and computers. You will be responsible for the purification of proteins in small to medium scale from baculovirus infected cells and mammalian cells and purification and refolding of proteins from E. coli and Pichia sources. You should possess a B.S., M.S. or MD degree in Biochemistry or medical sciences. Our client is a leading biotech firm with research facilities in New York and can provide excellent benefits (health insurance, dental, and vision plan, paid vacation and more). A high impact, high profile position with excellent opportunity for advancement. Geographic Location of Position: US-New York If you know anyone that might be interested, please forward this to them or contact: Sandy Sachs DMC Voice: 609-584-8733 Ext. 218 Fax: 609-584-9575 Email: 915608@candseek.com To permanently discontinue receiving employment opportunity notices from any and all help wanted advertisers using the Candidate Seeker system, click your "Reply" button and type the word "re- move" without spaces between the letters into the SUBJECT field then click the "Send" button. Your email address will be permanently filtered from ALL future job opportunity notifications sent via the Candidate Seeker system. To temporarily filter employment opportunity notices sent via the Candidate Seeker system, type the acronym "JOBOP" into your subject filter. All employment opportunity notices sent via the Candidate Seeker system contain the acronym "JOBOP" in the subject so they may be easily filtered or blocked if so desired. Other email addresses may be permanently deleted from future contact by emailing a single blank message from the desired address to nomail@candseek.com. Enter additional addresses into the body of the message and they will also be added to the "nomail" list Please feel free to contact the candidateseeker.com feedback line at 609-584-5499. Do not use this number for job related questions. All job related questions should be directed to the employer by replying to contact addresses or phone numbers indicated at the end of the job description message. From owner-biophysics@net.bio.net Wed Jul 21 19:24:00 1999 Path: biosci!candseek.com!915608 From: 915608@candseek.com Newsgroups: bionet.biophysics Subject: JOBOP RESEARCH ASSOCIATE PROTEIN PURIFICATION Date: 21 Jul 1999 13:24:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 69 Sender: daemon@net.bio.net Distribution: world Message-ID: <199907212024.NAA25451@net.bio.net> NNTP-Posting-Host: net.bio.net Since your email address was listed on a related web site page or database, I thought you might help. I am seeking an individual within the following conditions: a person to perform protein purification. The candidate will have 1+ years experience with protein purification (general chromatography skills) and characterization (SDS Page, Western Blot, slot/dot blot, HPLC). Familiarity with UV VIS spectroscopy and computers. You will be responsible for the purification of proteins in small to medium scale from baculovirus infected cells and mammalian cells and purification and refolding of proteins from E. coli and Pichia sources. You should possess a B.S., M.S. or MD degree in Biochemistry or medical sciences. Our client is a leading biotech firm with research facilities in New York and can provide excellent benefits (health insurance, dental, and vision plan, paid vacation and more). A high impact, high profile position with excellent opportunity for advancement. Geographic Location of Position: US-New York If you know anyone that might be interested, please forward this to them or contact: Sandy Sachs DMC Voice: 609-584-8733 Ext. 218 Fax: 609-584-9575 Email: 915608@candseek.com To permanently discontinue receiving employment opportunity notices from any and all help wanted advertisers using the Candidate Seeker system, click your "Reply" button and type the word "re- move" without spaces between the letters into the SUBJECT field then click the "Send" button. Your email address will be permanently filtered from ALL future job opportunity notifications sent via the Candidate Seeker system. To temporarily filter employment opportunity notices sent via the Candidate Seeker system, type the acronym "JOBOP" into your subject filter. All employment opportunity notices sent via the Candidate Seeker system contain the acronym "JOBOP" in the subject so they may be easily filtered or blocked if so desired. 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From owner-biophysics@net.bio.net Wed Jul 21 19:46:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!news.u.washington.edu!fujimoto From: fujimoto@u.washington.edu (Bryant Fujimoto) Newsgroups: bionet.biophysics Subject: Re: static light scattering and M.W. measurement Date: 21 Jul 1999 20:33:34 GMT Organization: University of Washington Lines: 30 Message-ID: <7n5aqu$15qa$1@nntp4.u.washington.edu> References: <3795885E.7C03ACD6@mrc-lmb.cam.ac.uk> NNTP-Posting-Host: homer23.u.washington.edu X-Trace: nntp4.u.washington.edu 932589214 38730 (None) 140.142.17.40 X-Complaints-To: help@cac.washington.edu NNTP-Posting-User: fujimoto Yu Wai Chen writes: >Dear all, >It seems that Protein Solution's discussion forum is not a very active >one and questions posted there simply get no answer. I'll try this one. >I tried to measure accurate M.W. of my protein using static light >scattering so I made light scattering measurements with sample in >various concentration and do the plot. From this total intensity light >scattering experiment I obtained a value which is quite close to the >calculated M.W. of my protein. >However, I know that my protein exists as dimer in the condition I used >for these experiments. From dynamic light scattering experiments of the >same samples, I obtained M.W. corresponding to a dimer. So DLS gives me >M.W. that is two times the M.W. obtained from SLS. >My question is, does the SLS method always give monomeric M.W.? Are my >results correct? >In concentration calculations in the SLS plot, I used the monomeric M.W. Just a thought, how big is your protein? If it is comparable in size to the wavelength of light, you need to extrapolate not just to zero concentration, but also to zero scattering angle. Bryant Fujimoto fujimoto@u.washington.edu -- From owner-biophysics@net.bio.net Thu Jul 22 08:27:00 1999 Path: biosci!CHEM.UFL.EDU!tan From: tan@CHEM.UFL.EDU (Weihong Tan) Newsgroups: bionet.biophysics Subject: Research Associate Positions in Bioanalytical and Biomedical Engineering Date: 22 Jul 1999 02:27:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Research associate positions at the Department of Chemistry and UF Brain Institute, University of Florida Bioanalytical Chemistry and Biomedical Engineering Applications are invited for one or two postdoctoral positions at the Department of Chemistry and at the UF Brain Institute, University of Florida. The successful applicant should have a strong background in ANY ONE of the following areas: Bioanalytical Chemistry; Biophysics; Biomedical Engineering; Neuroscience. Experiences in any one of the following fields are highly desired: biochemical sensor development, optical imaging of biological samples, scanning probe microscopy, or DNA/RNA/protein studies. We are carrying out exciting research work in bioanalytical chemistry, biomedical engineering and biophysics. These research activities are funded by the US National Science, The Whitaker Foundation for Biomedical Engineering, the Office of Naval Research, Department of Defense and The Arnold Beckman Foundation and by industrial companies. Our group has labs both in the Chemistry Department and in the newly established UF Brain Institute. This Institute is a national center for neuroscience and brain research. At the University of Florida we have excellent working and living environment. The weather at Gainesville is excellent, and there are many outdoor activities and cultural events throughout the year. Interested applicants should make an initial contact with Prof. Tan through e-mail, or send a CV to the following address: Prof. Weihong Tan Department of Chemistry and The UF Brain Institute University of Florida, Gainesville, FL 32611-7200 e-mail: tan@chem.ufl.edu; Tel: 352-846-2410; Fax: 352-392-4651 Web page: http://www.chem.ufl.edu/Groups/Tan/ From owner-biophysics@net.bio.net Thu Jul 22 20:26:00 1999 Path: biosci!mayo.edu!luo.zong From: luo.zong@mayo.edu (Zong-Ping Luo) Newsgroups: bionet.biophysics Subject: Postdoctoral position Date: 22 Jul 1999 14:26:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 54 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.5.32.19990722161840.009853a0@fermat.mayo.edu> NNTP-Posting-Host: net.bio.net
MAYO CLINIC POSTDOCTORAL POSITION IN CELLULAR AND MOLECULAR BIOMECHANICS
The Mayo Clinic is pleased to announce a postdoctoral fellowship in cellular and molecular biomechanics. This fellowship is a collaborative effort of the Orthopedic Biomechanics Laboratory and the Orthopedic Molecular Biology Laboratory and provides an opportunity for full-time research in micro-level biomechanics. Eligibility: Ph.D.'s, or equivalent training in cell biology, biochemistry, biophysics, molecular biology, or tissue engineering. Research Areas: Develop cross-disciplined research in mechanical properties and functions of extracellular matrix and interaction between cells and extracellular matrix at both cellular and molecular levels. Applicants should submit a curriculum vitae, a statement of interest, and three referee names to: Dr. Zong-Ping Luo Orthopedic Biomechanics Laboratory 128 Guggenheim Building Mayo Clinic Rochester, MN 55905 Fax: (507) 284-5392 E-mail: luo.zong@mayo.edu Mayo Clinic/Mayo Foundation is an affirmative action and equal opportunity educator and employer. From owner-biophysics@net.bio.net Fri Jul 23 13:31:00 1999 Path: biosci!newshost.lanl.gov!logbridge.uoregon.edu!news-peer.gip.net!news.gsl.net!gip.net!hub1.ispnews.com!news-master.service.talkway.com!c01read02-admin.service.talkway.com.POSTED!not-for-mail From: "irpeterson" Subject: UK Postgraduate Studentship available in biosensors Newsgroups: bionet.biophysics X-Client-NNTP-Posting-Host: d347-1.coventry.ac.uk/194.66.39.67 Followup-To: bionet.biophysics X-TWRN-Tag: 932739757369 Lines: 40 Message-ID: Date: Fri, 23 Jul 1999 14:25:11 GMT NNTP-Posting-Host: 216.200.3.202 X-Trace: c01read02-admin.service.talkway.com 932739911 216.200.3.202 (Fri, 23 Jul 1999 07:25:11 PDT) NNTP-Posting-Date: Fri, 23 Jul 1999 07:25:11 PDT Organization: Talkway, Inc. Coventry University School of Natural and Environmental Sciences A 3-year Graduate Studentship leading to PhD Degree BIOPHYSICS: MEMBRANE BIOSENSOR This EPSRC-funded project involves the study of biomimetic membranes in a novel and promising configuration which will lead to a range of biosensors of extreme sensitivity and selectivity. The principle has already been demonstrated, and you will take the work on towards practical clinical applications. The work will be highly interdisciplinary. You should possess a degree in an appropriate discipline, which might be physics, biology or biochemistry, and you should be a citizen of the UK. The project will be carried out in the Coventry Centre for Molecular and Biomolecular Electronics, and there will also be strong interaction with Dr Bion and Dr Garnham at the Queen Elizabeth Hospital in Birmingham. The standard EPSRC salary of ~5.5K can be supplemented by part-time teaching to the extent of ~1.5K. For more details consult the CMBE website on http://www.nes.coventry.ac.uk/research/cmbe/epsrcbiosens.htm or ring Professor I R Peterson on (01203) 838376. Available immediately. -- Posted via Talkway - http://www.talkway.com Exchange ideas on practically anything (tm). From owner-biophysics@net.bio.net Fri Jul 23 13:36:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!arclight.uoregon.edu!wn4feed!worldnet.att.net!128.230.129.106!news.maxwell.syr.edu!nntp2.deja.com!nnrp1.deja.com!not-for-mail From: irpeterson@my-deja.com Newsgroups: bionet.biophysics Subject: Postgraduate studentship available in biosensors Date: Fri, 23 Jul 1999 14:16:42 GMT Organization: Deja.com - Share what you know. Learn what you don't. Lines: 40 Message-ID: <7n9tg0$n65$1@nnrp1.deja.com> NNTP-Posting-Host: 194.66.39.67 X-Article-Creation-Date: Fri Jul 23 14:16:42 1999 GMT X-Http-User-Agent: Mozilla/4.51 [en] (Win95; I) X-Http-Proxy: 1.0 x25.deja.com:80 (Squid/1.1.22) for client 194.66.39.67 X-MyDeja-Info: XMYDJUIDirpeterson Coventry University School of Natural and Environmental Sciences A 3-year Graduate Studentship leading to PhD Degree BIOPHYSICS: MEMBRANE BIOSENSOR This EPSRC-funded project involves the study of biomimetic membranes in a novel and promising configuration which will lead to a range of biosensors of extreme sensitivity and selectivity. The principle has already been demonstrated, and you will take the work on towards practical clinical applications. The work will be highly interdisciplinary. You should possess a degree in an appropriate discipline, which might be physics, biology or biochemistry, and you should be a citizen of the UK. The project will be carried out in the Coventry Centre for Molecular and Biomolecular Electronics, and there will also be strong interaction with Dr Bion and Dr Garnham at the Queen Elizabeth Hospital in Birmingham. The standard EPSRC salary of ~5.5K can be supplemented by part-time teaching to the extent of ~1.5K. For more details consult the CMBE website on http://www.nes.coventry.ac.uk/research/cmbe/epsrcbiosens.htm or ring Professor I R Peterson on (01203) 838376. Available immediately. Sent via Deja.com http://www.deja.com/ Share what you know. Learn what you don't. From owner-biophysics@net.bio.net Fri Jul 23 15:46:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!news.maxwell.syr.edu!nntp2.deja.com!nnrp1.deja.com!not-for-mail From: irpeterson@my-deja.com Newsgroups: bionet.biophysics Subject: UK Postgraduate Studentship in Biosensors Date: Fri, 23 Jul 1999 16:34:33 GMT Organization: Deja.com - Share what you know. Learn what you don't. Lines: 40 Message-ID: <7na5io$ps5$1@nnrp1.deja.com> NNTP-Posting-Host: 194.66.39.67 X-Article-Creation-Date: Fri Jul 23 16:34:33 1999 GMT X-Http-User-Agent: Mozilla/4.51 [en] (Win95; I) X-Http-Proxy: 1.0 x22.deja.com:80 (Squid/1.1.22) for client 194.66.39.67 X-MyDeja-Info: XMYDJUIDirpeterson Coventry University School of Natural and Environmental Sciences A 3-year Graduate Studentship leading to PhD Degree BIOPHYSICS: MEMBRANE BIOSENSOR This EPSRC-funded project involves the study of biomimetic membranes in a novel and promising configuration which will lead to a range of biosensors of extreme sensitivity and selectivity. The principle has already been demonstrated, and you will take the work on towards practical clinical applications. The work will be highly interdisciplinary. You should possess a degree in an appropriate discipline, which might be physics, biology or biochemistry, and you should be a citizen of the UK. The project will be carried out in the Coventry Centre for Molecular and Biomolecular Electronics, and there will also be strong interaction with Dr Bion and Dr Garnham at the Queen Elizabeth Hospital in Birmingham. The standard EPSRC salary of ~5.5K can be supplemented by part-time teaching to the extent of ~1.5K. For more details consult the CMBE website on http://www.nes.coventry.ac.uk/research/cmbe/epsrcbiosens.htm or ring Professor I R Peterson on (01203) 838376. Available immediately. Sent via Deja.com http://www.deja.com/ Share what you know. Learn what you don't. From owner-biophysics@net.bio.net Fri Jul 23 15:56:00 1999 Path: biosci!newshost.lanl.gov!logbridge.uoregon.edu!news.maxwell.syr.edu!nntp2.deja.com!nnrp1.deja.com!not-for-mail From: irpeterson@my-deja.com Newsgroups: bionet.biophysics Subject: UK Postgraduate Studentship in Biosensors Date: Fri, 23 Jul 1999 16:43:51 GMT Organization: Deja.com - Share what you know. Learn what you don't. Lines: 40 Message-ID: <7na644$q2u$1@nnrp1.deja.com> NNTP-Posting-Host: 194.66.39.67 X-Article-Creation-Date: Fri Jul 23 16:43:51 1999 GMT X-Http-User-Agent: Mozilla/4.51 [en] (Win95; I) X-Http-Proxy: 1.0 x24.deja.com:80 (Squid/1.1.22) for client 194.66.39.67 X-MyDeja-Info: XMYDJUIDirpeterson Coventry University School of Natural and Environmental Sciences A 3-year Graduate Studentship leading to PhD Degree BIOPHYSICS: MEMBRANE BIOSENSOR This EPSRC-funded project involves the study of biomimetic membranes in a novel and promising configuration which will lead to a range of biosensors of extreme sensitivity and selectivity. The principle has already been demonstrated, and you will take the work on towards practical clinical applications. The work will be highly interdisciplinary. You should possess a degree in an appropriate discipline, which might be physics, biology or biochemistry, and you should be a citizen of the UK. The project will be carried out in the Coventry Centre for Molecular and Biomolecular Electronics, and there will also be strong interaction with Dr Bion and Dr Garnham at the Queen Elizabeth Hospital in Birmingham. The standard EPSRC salary of ~5.5K can be supplemented by part-time teaching to the extent of ~1.5K. For more details consult the CMBE website on http://www.nes.coventry.ac.uk/research/cmbe/epsrcbiosens.htm or ring Professor I R Peterson on (01203) 838376. Available immediately. Sent via Deja.com http://www.deja.com/ Share what you know. Learn what you don't. From owner-biophysics@net.bio.net Sat Jul 24 19:45:00 1999 Path: biosci!HOTBOT.COM!fvwgysosjs From: fvwgysosjs@HOTBOT.COM Newsgroups: bionet.biophysics Subject: ACCEPT CREDIT CARDS OVER THE INTERNET.....NO SETUP FEES!! 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From owner-biophysics@net.bio.net Mon Jul 26 13:40:00 1999 Path: biosci!pravda.ucr.edu!awabi.library.ucla.edu!128.230.129.106!news.maxwell.syr.edu!news.idt.net!nntp.farm.idt.net!news From: "NDRI/HBDI" Newsgroups: alt.bio.technology,alt.bio.technology.misc,bionet,bionet.biology,bionet.biophysics,bionet.cellbiol Subject: Autopsy, Surgical Discard and Transplantation Tissues for Now Available for Research Date: Mon, 26 Jul 1999 10:20:28 -0400 Organization: IDT (Best News In The World) Lines: 16 Message-ID: <7nhr58$bo8@nnrp4.farm.idt.net> NNTP-Posting-Host: ppp-23.ts-1.ph.idt.net X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2314.1300 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2314.1300 Xref: biosci bionet.biophysics:5126 bionet.cellbiol:12193 See www.ndri.com for access to human tissues and organs for medical researchers See www.hbdi.org for information about family collections containing DNA and immortalized cells - over 600 families - for medical and genetic researchers NDRI and HBDI are non-profit organizations aiding medical research and the search for disease genes. From owner-biophysics@net.bio.net Mon Jul 26 21:46:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!arclight.uoregon.edu!wn4feed!worldnet.att.net!199.60.229.5!newsfeed.direct.ca!newspeer.monmouth.com!newsfeed1.earthlink.net!nntp.earthlink.net!posted-from-earthlink!not-for-mail From: John Philo <"jphilo*NO SPAM12*"@earthlink.net> Newsgroups: bionet.biophysics Subject: Re: static light scattering and M.W. measurement Date: Mon, 26 Jul 1999 15:31:38 -0700 Content-Transfer-Encoding: 7bit References: <3795885E.7C03ACD6@mrc-lmb.cam.ac.uk> To: Yu Wai Chen X-Posted-Path-Was: not-for-mail Content-Type: text/plain; charset=us-ascii X-ELN-Date: 26 Jul 1999 22:31:53 GMT X-ELN-Insert-Date: Mon Jul 26 15:35:05 1999 Organization: Alliance Protein Laboratories Lines: 52 Mime-Version: 1.0 NNTP-Posting-Host: 1cust149.tnt2.thousand-oaks.ca.da.uu.net Message-ID: <379CE1C9.396903D6@earthlink.net> X-Mailer: Mozilla 4.05 [en] (Win95; U) Dynamic light scattering NEVER really measures molecular mass (something the Protein Solutions sales people won't admit). DLS can only estimate mass based on an assumption that your protein is a sphere (and thus is no better at absolute masses than running a size exclusion column versus globular protein standards). If your protein is an ASYMMETRIC monomer than DLS would indicate that it is a dimer. In principle the total intensity data (static LS) should always give the correct solution mass (i.e. reflecting any self-association that may occur), provided the physical size is small compared to the wave length of light (which will be true for globular proteins less than about 500 kDa), and provided the calibration has been done correctly. You may want to check your SLS calibration using a protein standard. BSA and ovalbumin are problematic because they always contain some oligomer, but they would certainly tell you if the calibration is off by a factor of 2, and you could also try something like ribonuclease. John Philo Alliance Protein Laboratories Yu Wai Chen wrote: > > Dear all, > > It seems that Protein Solution's discussion forum is not a very active > one and questions posted there simply get no answer. I'll try this one. > > I tried to measure accurate M.W. of my protein using static light > scattering so I made light scattering measurements with sample in > various concentration and do the plot. From this total intensity light > scattering experiment I obtained a value which is quite close to the > calculated M.W. of my protein. > > However, I know that my protein exists as dimer in the condition I used > for these experiments. From dynamic light scattering experiments of the > same samples, I obtained M.W. corresponding to a dimer. So DLS gives me > M.W. that is two times the M.W. obtained from SLS. > > My question is, does the SLS method always give monomeric M.W.? Are my > results correct? > > In concentration calculations in the SLS plot, I used the monomeric M.W. > > Thanks a lot. > > -- > =================================================================== > Yu Wai CHEN, Ph.D. .................. email:ywc@mrc-lmb.cam.ac.uk > Centre for Protein Engineering, tel:+44-(0)1223-402148 > MRC Centre, Hills Rd, Cambridge CB2 2QH, UK fax:+44-(0)1223-402140 > WWW homepage: http://www.mrc-cpe.cam.ac.uk/~ywc From owner-biophysics@net.bio.net Tue Jul 27 04:48:00 1999 Path: biosci!COMPUSERVE.COM!4357.2104 From: 4357.2104@COMPUSERVE.COM Newsgroups: bionet.biophysics Subject: Increase Profits! Date: 26 Jul 1999 22:48:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: <199907270548.WAA17169@net.bio.net> NNTP-Posting-Host: net.bio.net Increase your Merchant Sales Don't loose valuable sales by not providing Visa, M/C, American Express and Discover Card acceptance! .EZ CREDIT Approval .Low Transaction Fees .Minimum Start-Up Costs .On-Line real Time Processing .Equipment Lease-Option Plans .Swipe Rates As Low As 1.71% (+20 cents/transaction) .Keyed Rates As Low As 2.50% (+25 cents/transaction) .Deposits Direct To Your Existing Bank Account ******Act now and all App. & Processing fees waived***** Credit Card Services 150 offices nationwide over 1,500 sales representatives Call us today @ 1 (888) 869-5520 and ask for extention #909 for free setup! dj760@excite.com From owner-biophysics@net.bio.net Tue Jul 27 14:56:00 1999 Path: biosci!newshost.lanl.gov!logbridge.uoregon.edu!dispose.news.demon.net!demon!newsfeed.icl.net!news.algonet.se!algonet!masternews.telia.net!newsb.telia.net.POSTED!not-for-mail From: "Eva Åkesson" Newsgroups: bionet.biophysics Subject: Winterschool Ultrafast Spectroscopy in Chemistry and Biology X-Newsreader: Microsoft Outlook Express 4.72.3110.5 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.3110.3 Message-ID: Date: Tue, 27 Jul 1999 15:50:35 GMT NNTP-Posting-Host: 195.67.194.133 X-Complaints-To: abuse@telia.com X-Trace: newsb.telia.net 933090635 195.67.194.133 (Tue, 27 Jul 1999 17:50:35 CEST) NNTP-Posting-Date: Tue, 27 Jul 1999 17:50:35 CEST Organization: Telia Internet Lines: 33 Borgafjäll IV in Sweden Ultrafast Spectroscopy in Chemistry and Biology 9/4 - 15/4 2000 We would like to announce the fourth Borgafjäll winterschool! Borgafjäll is situated in the northwest of Sweden in the mountains close to Norway. This time of the year is the best skiing season and the lectures will be organized in such a way that there will be opportunities for skiing, both downhill and cross-country is possible. The school and accommodation will be in a ski-resort hotel having all normal facilities, including swimming pool, sauna, etc. The objective of the Borgafjäll Winter School is to review the most recent progress in ultrafast spectroscopy and related methods and to train young researchers with varying scientific backgrounds in new chemical and biological applications made possible through this new development. In a truly multidisciplinary atmosphere, young researchers will be guided toward new frontiers within their respective fields by some of Europe's most experienced scientists in ultrafast spectroscopy applications. The new technological developments have made possible very strong scientific progress in areas such as: chemical reaction dynamics and control in isolated, cluster, and condensed-phase molecular systems; photosynthetic energy conversion; structure and dynamics of macromolecular systems; organized molecular systems for new materials; materials for electronics and communications. For more information: http://www.chemphys.lu.se/courses/Borgafjall/index.htm or contact Dr. Eva Åkesson, eva.akesson@chemphys.lu.se From owner-biophysics@net.bio.net Tue Jul 27 23:29:00 1999 Path: biosci!agate!newsfeed.berkeley.edu!newsfeed.gamma.ru!Gamma.RU!tank.news.pipex.net!pipex!uunet!zur.uu.net!ffx.uu.net!dfw.uu.net!chi.uu.net!typhoon.stlnet.com!not-for-mail Message-ID: <379E4EFA.B85CA82@yahoo.com> From: Jeff Seale X-Mailer: Mozilla 4.6 [en] (Win95; I) X-Accept-Language: en MIME-Version: 1.0 Newsgroups: bionet.biophysics Subject: Re: static light scattering and M.W. measurement References: <3795885E.7C03ACD6@mrc-lmb.cam.ac.uk> <379CE1C9.396903D6@earthlink.net> Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 49 Date: Tue, 27 Jul 1999 19:29:46 -0500 NNTP-Posting-Host: 209.96.4.100 X-Complaints-To: news@postnet.com X-Trace: typhoon.stlnet.com 933121412 209.96.4.100 (Tue, 27 Jul 1999 19:23:32 CDT) NNTP-Posting-Date: Tue, 27 Jul 1999 19:23:32 CDT Organization: Pulitzer Technologies, INC. I typically use DLS to get a rough idea of the size of the protein particle and make a guess at the MW. It is also very useful to scout conditions for analytical ultracentrifugation runs which can give you the best estimate of the avg MW of your protein, as well as the association constant for the oligomerization. In one situation I have now, DLS indicates something that is on the order of the size of a tetramer. However, from the crystal structure, this protein cam form a dimer which would be quite extended, almost like a butterfly shaped. So, DLS can't tell me if I have a "spherical" tetramer, or and extended dimer. So, it's off to the ultracentrifuge. -Jeff > > Yu Wai Chen wrote: > > > > Dear all, > > > > It seems that Protein Solution's discussion forum is not a very active > > one and questions posted there simply get no answer. I'll try this one. > > > > I tried to measure accurate M.W. of my protein using static light > > scattering so I made light scattering measurements with sample in > > various concentration and do the plot. From this total intensity light > > scattering experiment I obtained a value which is quite close to the > > calculated M.W. of my protein. > > > > However, I know that my protein exists as dimer in the condition I used > > for these experiments. From dynamic light scattering experiments of the > > same samples, I obtained M.W. corresponding to a dimer. So DLS gives me > > M.W. that is two times the M.W. obtained from SLS. > > > > My question is, does the SLS method always give monomeric M.W.? Are my > > results correct? > > > > In concentration calculations in the SLS plot, I used the monomeric M.W. > > > > Thanks a lot. > > > > -- > > =================================================================== > > Yu Wai CHEN, Ph.D. .................. email:ywc@mrc-lmb.cam.ac.uk > > Centre for Protein Engineering, tel:+44-(0)1223-402148 > > MRC Centre, Hills Rd, Cambridge CB2 2QH, UK fax:+44-(0)1223-402140 > > WWW homepage: http://www.mrc-cpe.cam.ac.uk/~ywc From owner-biophysics@net.bio.net Fri Jul 30 03:55:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!hermes.visi.com!news-out.visi.com!nntp.giganews.com!nntp2.giganews.com!news2.giganews.com.POSTED!not-for-mail Message-ID: <37A12E0C.54BF7E2@hotmail.com> From: Kermit The Frog X-Mailer: Mozilla 4.61 [en] (Win95; I) X-Accept-Language: de-DE,fr-FR MIME-Version: 1.0 Newsgroups: bionet.biophysics Subject: xray sources question Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 7 NNTP-Posting-Date: Thu, 29 Jul 1999 23:49:24 CDT Organization: Giganews.Com - Premium News Outsourcing X-Trace: sv1-OSn6xlhPDO1/iiomd04BaiuIq/HVbwH0frICkM1J1X01U8iU4sgSNnk5Fab9Gmjm4VoPtNCsRg1cIz+!R7bAntrByrw= X-Complaints-To: abuse@GigaNews.Com X-Abuse-Info: Please be sure to forward a copy of ALL headers X-Abuse-Info: Otherwise we will be unable to process your complaint properly Date: Thu, 29 Jul 1999 21:46:04 -0700 I noticed that there is an overview on femptosecond x-ray (laser?) sources covered in the afore mentioned winterschool. Has anyone everbuilt something like that and would it be a realistic undertaking for a graduate student? Kermit