From owner-biophys@hgmp.mrc.ac.uk Mon May 1 09:20:00 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id AC39B17A89; Mon, 1 May 2000 09:19:59 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id JAA19418 for ; Mon, 1 May 2000 09:19:57 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id JAA14244 for biophys-list@hgmp.mrc.ac.uk; Mon, 1 May 2000 09:19:56 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: "leejoe" X-Newsgroups: bionet.biophysics Subject: Postdoctoral Position available on K+ channels at Academia Sinica/Taiwan Date: Mon, 1 May 2000 16:20:59 +0800 Organization: Computing Center, Academia Sinica Lines: 17 Message-ID: <8ejenb$c3$1@news1.sinica.edu.tw> X-Trace: news1.sinica.edu.tw 957169195 387 140.109.41.70 (1 May 2000 08:19:55 GMT) X-Complaints-To: usenet@news.sinica.edu.tw X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2314.1300 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2314.1300 To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk From: ruchi@ibms.sinica.edu.tw Postdoctoral Fellow position is available at the Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. Our laboratory is studying gating and permeating properties of the cloned inward rectifier K+ channels, Kir2.1. The desirable candidate will have some graduate training in electrophysiology with particular expertise in some or all of the following methods: patch clamp site-directed mutagenesis fluorescence measurements. From owner-biophys@hgmp.mrc.ac.uk Tue May 2 19:59:32 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 8B62517AFC; Tue, 2 May 2000 19:59:31 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id TAA07771 for ; Tue, 2 May 2000 19:59:29 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id TAA18093 for biophys-list@hgmp.mrc.ac.uk; Tue, 2 May 2000 19:59:28 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: actnow123@mail.com X-Newsgroups: bionet.biophysics Subject: WE CAN HELP YOU GET EXPOSED, WITH BULK E-MAIL Date: 2 May 2000 19:59:28 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 31 Message-ID: <200005021859.TAA07764@hgmp.mrc.ac.uk> X-Trace: niobium.hgmp.mrc.ac.uk 957293968 18091 193.62.192.80 (2 May 2000 18:59:28 GMT) X-Complaints-To: news@net.bio.net X-Received: from lotus.ariel.com (lotus.ariel.com [204.249.107.2]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with SMTP id TAA07764 for ; Tue, 2 May 2000 19:59:26 +0100 (BST) X-Received: from cranmail.ariel.com by lotus.ariel.com via smtpd (for mercury.hgmp.mrc.ac.uk [193.62.192.80]) with SMTP; 2 May 2000 19:47:40 UT X-Received: from lotus.ariel.com ([10.1.0.2]) by cranmail.ariel.com with SMTP (Microsoft Exchange Internet Mail Service Version 5.5.2650.21) id K10WY5V7; Tue, 2 May 2000 14:59:51 -0400 X-Received: from PPPa96-ResaleModesto1-4R7275.saturn.bbn.com ([4.16.191.157]) by lotus.ariel.com via smtpd (for cranmail.ariel.com [10.1.0.181]) with SMTP; 2 May 2000 19:45:49 UT X-Reply-to: actnow123@mail.com X-TO: actnow123@mail.com To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk I am sorry if this ad as offended you. 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METHOD OF PAYMENT , CASHIERS CHECK MONEY ORDER OR BANK WIRE. --- From owner-biophys@hgmp.mrc.ac.uk Wed May 3 13:52:36 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id E48F317B70; Wed, 3 May 2000 13:52:11 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id NAA04922; Wed, 3 May 2000 13:52:07 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id NAA09777; Wed, 3 May 2000 13:52:04 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: "NDRI HBDI" X-Newsgroups: alt.bio.technology,alt.bio.technology.misc,bionet,bionet.biology,bionet.biophysics,bionet.cellbiol,bionet.biology.cardiovascular Subject: Autopsy, Surgical Discard and Transplantation Tissues for Now Available for Research Date: Wed, 3 May 2000 08:41:08 -0400 Organization: IDT (Best News In The World) Lines: 18 Message-ID: <8ep77a$sef@nnrp3.farm.idt.net> X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2615.200 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2615.200 To: biophys@hgmp.mrc.ac.uk, cardiors@hgmp.mrc.ac.uk, cellbiol@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk See www.ndri.com for access to human tissues and organs for medical researchers See www.hbdi.org for information about family collections containing DNA and immortalized cells - over 700 families - for medical and genetic researchers NDRI and HBDI are non-profit organizations aiding medical research and the search for disease genes. From owner-biophys@hgmp.mrc.ac.uk Thu May 4 05:53:13 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 1E7D517A9C; Thu, 4 May 2000 05:53:12 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id FAA13948 for ; Thu, 4 May 2000 05:53:11 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id FAA29842 for biophys-list@hgmp.mrc.ac.uk; Thu, 4 May 2000 05:53:10 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: freedom4ever@china.com X-Newsgroups: bionet.biophysics Subject: Do You Like Your J.O.B?? Date: 4 May 2000 05:53:09 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 100 Message-ID: <200005040450.AAA01370@isd.jhpiego.jhu.edu> Mime-Version: 1.0 Content-Type: multipart/mixed; boundary="----=_NextPart_000_018B_00007A5B.00005867" X-Trace: niobium.hgmp.mrc.ac.uk 957415989 29840 193.62.192.80 (4 May 2000 04:53:09 GMT) X-Complaints-To: news@net.bio.net X-Received: from isd.jhpiego.jhu.edu ([128.220.176.51]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id FAA13940 for ; Thu, 4 May 2000 05:53:06 +0100 (BST) X-Received: from erik (max1-20.atlanta.corecomm.net [216.214.97.148]) by isd.jhpiego.jhu.edu (2.5 Build 2630 (Berkeley 8.8.6)/8.8.4) with SMTP id AAA01370; Thu, 04 May 2000 00:50:17 -0400 X-To: X-Priority: 3 X-MSMail-Priority: Normal X-Reply-To: nancy@china.com To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk ------=_NextPart_000_018B_00007A5B.00005867 Content-Type: text/html;
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--- From owner-biophys@hgmp.mrc.ac.uk Thu May 4 12:47:37 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id D6C7917A62; Thu, 4 May 2000 12:47:35 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id MAA16456 for ; Thu, 4 May 2000 12:47:32 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id MAA05031 for biophys-list@hgmp.mrc.ac.uk; Thu, 4 May 2000 12:47:31 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: mriehle@udcf.gla.ac.uk (Mathis Riehle) X-Newsgroups: bionet.biophysics Subject: 2 Postdoctoral Position available Date: Thu, 04 May 2000 12:09:56 +0100 Organization: Centre for Cell Engineering Lines: 47 Message-ID: X-Newsreader: MT-NewsWatcher 2.4.4 To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk Two Post-Doctoral positions available in the Centre for Cell Engineering University of Glasgow 1. Micromechanics of Cells The aim is to design, produce and use equipment for measuring the mechanical forces that can be generated by single tissue cells. The post should suit a biophysicist, or some one with experience of microfabrication techniques and an interest in biological systems. Access to the fabrication facilities of the Department of Electronics & Electrical Engineering will be available. The post is part of an EC funded Framework V network with other partners at Keele, UCL, Bordeaux, Linkopping, Genoa and in the Netherlands. The other partners are working more on the biological effects of mechanical forces on cells. The person appointed to this post would be expected to take part in the collaboration between the various partners perhaps by travelling to other laboratories in the group. Preliminary work done by a Ph.D student has already lead to a device in silicon for this purpose but further developments are desirable. Experience in biophysics and/or microfabrication are desirable. There is joint ongoing work with laboratories outside Western Europe as well 2. Bioelectronic retina This post is set out to test and develop methods of data acquisition from large scale (64+) micro-electrode arrays interfaced with retinae. This area is amied at vision prostheses and is the European end of a USA/Europe collaboration. The person appointed will join a team with members from the department of Physics, Department of Electronics and Electrical Engineering and from the Centre for Cell Engineering. Experience in signal acquisition and processing whether in biology or in other areas would be a desirable qualification for this post. Microfabrication will be used in the project. A lot of work has already been done in this lab on the fabrication side of this subject. (2 year post). Send applications including a CV please to: Professor Adam Curtis Centre for Cell Engineering, Joseph Black Building University of Glasgow Glasgow G12 8QQ Scotland. Informal enquiries via e-mail: A.Curtis@bio.gla.ac.uk The pay scale is in the RA1A range, starting point depends on age and experience (e.g. Ł18185 at age 27). From owner-biophys@hgmp.mrc.ac.uk Fri May 5 23:57:38 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 8A44E17A5B; Fri, 5 May 2000 23:57:36 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id XAA20244 for ; Fri, 5 May 2000 23:57:34 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id XAA17834 for biophys-list@hgmp.mrc.ac.uk; Fri, 5 May 2000 23:57:32 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: freedom4ever@china.com X-Newsgroups: bionet.biophysics Subject: Knowledge You Deserve!! Date: 5 May 2000 23:57:29 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 100 Message-ID: <200005052252.IAA23219@stinger.tds.com.au> Mime-Version: 1.0 Content-Type: multipart/mixed; boundary="----=_NextPart_000_0E33_00002BA9.00001A74" X-Trace: niobium.hgmp.mrc.ac.uk 957567449 17831 193.62.192.80 (5 May 2000 22:57:29 GMT) X-Complaints-To: news@net.bio.net X-Received: from stinger.tds.com.au (stinger.tenix.com [203.103.237.13]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id XAA20224 for ; Fri, 5 May 2000 23:57:23 +0100 (BST) X-Received: (from smap@localhost) by stinger.tds.com.au (8.9.3/8.9.3) id IAA23219; Sat, 6 May 2000 08:52:32 +1000 (EST) X-Received: from max1-65.atlanta.corecomm.net(216.214.97.193) by stinger.tds.com.au via TENIX (V6) id xmag23179; Sat, 6 May 00 08:51:37 +1000 X-To: X-Priority: 3 X-MSMail-Priority: Normal X-Reply-To: nancy@china.com To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk ------=_NextPart_000_0E33_00002BA9.00001A74 Content-Type: text/html;
Dear Friend,

Let me ask you a very straightforward question and
before you answer, think about it for a moment.

When you have trouble making ends meet on today's
income, how do you expect to retire and live on
less money than you earn now?

Read the question again before you go on -
it is a very serious question.

If your income stopped today, how many
months could you survive in your current lifestyle
with no money coming in? What will be different in
your life two years from now if you continue
doing what you are doing now?

Corporate America has conditioned us to believe
that security comes from employment. Yet we hope
we never become the victims of downsizing, career
burn out, illness or injury. The frightening
reality for most people is the plastic in their
wallets determines the quality of their lives.

Ask yourself, are you.....

* Tired of working for someone else and getting
paid what THEY tell you you're worth?
* Do your credit card balances total more than
your bank accounts?
* Tired of the get rich quick on $5 fantasy
programs?
* Tired of the Multi Level marketing residual
income illusion?
* Looking for REAL wealth?

CALL NOW 1-800-968-2825

THEN LOOK AT THIS!!!

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Level marketing or franchise.
* Proven lead generation system brings qualified
prospects to you.
* Multiple 6 figure lifestyle realistically
attainable in the first year.
* Endless support in an environment of
REAL integrity.
* 80% profit on all sales paid directly to you.

Our team of professionals is looking for a few
quality individuals with a strong work ethic and
DESIRE to generate $2,000 to $5,000 per week from
the comfort of their home. With 5 different income
streams available we teach people how to achieve a
6 figure cash flow within the first 6 to 12 months
and how to retire for good within 2 to 4 years.

SERIOUS SELF-STARTERS WITH A STRONG DESIRE TO IMPROVE
THEIR IMMEDIATE FINANCIAL FUTURE CALL NOW...

1-800-968-2825

Success is a matter of choice....your choice always.

Prosperous regards!

To be removed from all future mailings reply to
this email Please type remove in the subject line.
Calling the 800 number will not get you removed.
Under Bill s.1618 TITLE III passed by the 105th
U.S. Congress this letter can not be considered
spam as long as we include:
Contact information & a Remove Link

--- From owner-biophys@hgmp.mrc.ac.uk Sat May 6 05:59:23 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 035AC17B1A; Sat, 6 May 2000 05:59:21 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id FAA15269 for ; Sat, 6 May 2000 05:59:18 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id FAA22026 for biophys-list@hgmp.mrc.ac.uk; Sat, 6 May 2000 05:59:17 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: Nick Nystrom X-Newsgroups: bionet.announce,bionet.biology.computational,bionet.biophysics Subject: Workshop: Structure Determination Using NMR, July 26-29, 2000 Date: 6 May 2000 05:59:16 +0100 Organization: Pittsburgh Supercomputing Center Lines: 27 Message-ID: <39130463.F05D0F4C@psc.edu> Reply-To: blankens@psc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: niobium.hgmp.mrc.ac.uk 957589157 22024 193.62.192.80 (6 May 2000 04:59:17 GMT) X-Complaints-To: news@net.bio.net X-Accept-Language: en X-Auth: PGPMoose V1.1 PGP bionet.announce iQCVAwUAOROmo6CIqsWvyF5FAQGXQAP+Jv+gxUPTQOqYuCEnCPQ1hD1Cgs1j7mx3 nLZvunLUq9Xk19fMlJI3HLMq/cAo9+ndyGIyEF3i9QSwlLT2hqugFLbDQl+T4EDT bCrJtQ+kocYhYg8wxIA/+zGRnyO15FiT7IHEyZXEQdGftVH9GW5VlOOEczF415pw Wi95F6proo8= =2Gx+ To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk ********************************************************************** PSC BIOMEDICAL INITIATIVE WORKSHOPS 2000 STRUCTURE DETERMINATION USING NMR JULY 26-29, 2000 Application Deadline: June 14, 2000 The objective of this workshop is to introduce participants to the different techniques for the elucidation of solution structures of biological macromolecules from nuclear magnetic resonance data. The programs AMBER and MORASS will be discussed. In addition to lectures, there will be extensive hands-on sessions. Participants are encouraged to work on the examples provided or on their own experimental data. No prior supercomputing experience is necessary. For specific details, including an electronic application form, see: http://www.psc.edu/biomed/workshops/workshops.htm#NMR If you have any questions, please contact Nancy Blankenstein at blankens@psc.edu or 412/268-4960 (FAX). Many thanks for your interest and help. ********************************************************************** From owner-biophys@hgmp.mrc.ac.uk Sat May 6 17:36:57 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 70B1617ABD; Sat, 6 May 2000 17:36:56 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id RAA06079 for ; Sat, 6 May 2000 17:36:54 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id RAA00329 for biophys-list@hgmp.mrc.ac.uk; Sat, 6 May 2000 17:36:54 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: spy2@post.com X-Newsgroups: bionet.biophysics Subject: INTERNET SPY Date: 6 May 2000 17:36:53 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 128 Message-ID: <200005061629.e46GT1623377@tot-tq.proxy.aol.com> Mime-Version: 1.0 Content-Type: text/plain; charset=unknown-8bit Content-Transfer-Encoding: 8bit X-Trace: niobium.hgmp.mrc.ac.uk 957631013 327 193.62.192.80 (6 May 2000 16:36:53 GMT) X-Complaints-To: news@net.bio.net X-Received: from rly-ip02.mx.aol.com (rly-ip02.mx.aol.com [152.163.225.160]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id RAA06072 for ; Sat, 6 May 2000 17:36:51 +0100 (BST) X-Received: from tot-tq.proxy.aol.com (tot-tq.proxy.aol.com [152.163.201.1]) by rly-ip02.mx.aol.com (8.8.8/8.8.8/AOL-5.0.0) with ESMTP id MAA19541; Sat, 6 May 2000 12:29:09 -0400 (EDT) X-Received: from 194.98.10.252 (98A89E49.ipt.aol.com [152.168.158.73]) by tot-tq.proxy.aol.com (8.10.0/8.10.0) with SMTP id e46GT1623377; Sat, 6 May 2000 12:29:02 -0400 (EDT) X-To: Friend@public.com X-Apparently-From: Redtodayred@cs.com To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk //////////////////////////////////////// one time mailing- no need to remove //////////////////////////////////////// CONFIDENTIAL INFORMATION YOU WANT TO KNOW. 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Only our qualified sales team will have access to your order information) *************************************************************************************************************************** "The Internet DeskTop Spy" ORDER FORM - PLEASE WRITE CLEARLY Name: ________________________________ Address: ________________________________ (Credit Card Billing) City, State, ZIP: ________________________________ Country: ______________ (International Orders) Phone Number: ______________ (In case we can't make out your order) TOTAL COST OF ORDER IS $20.00 ALL ORDERS WILL BE PROCESSED WITHIN 48 HOURS OF RECEIPT METHOD OF PAYMENT – ID Code AXXX [ ]Visa [ ]MasterCard Credit Card #: __________________________________ Exp Date: _______________ Signature: ____________________________ (Required) E-Mail Address: ____________________________ *(VERY IMPORTANT-PRINT CLEARLY!!) -NOTES: - This program will not work on Windows 3.11 and older. - Sorry, Mac versions not yet available. - DISCLAIMER - The seller of this powerful software resource will not be held responsible for how the purchaser chooses to use its resources. --- From owner-biophys@hgmp.mrc.ac.uk Mon May 8 08:26:12 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 5974E17A5D; Mon, 8 May 2000 08:26:12 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id IAA17733 for ; Mon, 8 May 2000 08:26:10 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id IAA14354 for biophys-list@hgmp.mrc.ac.uk; Mon, 8 May 2000 08:26:09 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: dnicolau@swin.edu.au (dan nicolau) X-Newsgroups: bionet.biophysics Subject: Senior Position in biomicroengineering Date: 8 May 2000 08:26:09 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 51 Message-ID: <39166C13.BEC81E0F@swin.edu.au> Mime-Version: 1.0 Content-Type: multipart/mixed; boundary="------------F983A3686B7195136E93095D" X-Trace: niobium.hgmp.mrc.ac.uk 957770769 14352 193.62.192.80 (8 May 2000 07:26:09 GMT) X-Complaints-To: news@net.bio.net X-Received: from lucy.cc.swin.edu.au (lucy.cc.swin.edu.au [136.186.1.111]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id IAA17724 for ; Mon, 8 May 2000 08:26:02 +0100 (BST) X-Received: from swin.edu.au ([136.186.89.250]) by lucy.cc.swin.edu.au (8.9.3/8.9.3) with ESMTP id RAA20932; Mon, 8 May 2000 17:25:56 +1000 (AEST) X-Mailer: Mozilla 4.72 [en] (WinNT; I) X-Accept-Language: en X-To: biophys@hgmp.mrc.ac.uk, dnicolau@swin.edu.au To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk This is a multi-part message in MIME format. --------------F983A3686B7195136E93095D Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit We are looking for a person with proven research capabilities in the area of bio-micro-engineering. The candidate should have an important research experience in the areas of molecular and/or cellular biophyiscs or/and (micro)electronic engineering applied to life sciences. In particular, proven research using microfabricated systems, Scanning Probe Microscopies or/and confocal microscopy with track record in publications will be highly valued. Knowledge in the areas of microfabrication and computer simulations (e.g. molecular modelling and/or computational fluid dynamics) would be a plus. Rank is open up to Senior Lecturer. Appointment is available starting from June 2000. Contact: A/Prof. Dan Nicolau Industrial Research Institute Swinburne Swinburne University of Technology PO Box 218, Hawthorn, Vic 3122, Australia ph +61-392145083 (work); fax +61-392145050 e-mail: dnicolau@swin.edu.au --------------F983A3686B7195136E93095D Content-Type: text/x-vcard; charset=us-ascii; name="dnicolau.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for dan nicolau Content-Disposition: attachment; filename="dnicolau.vcf" begin:vcard n:Nicolau;Dan tel;fax:+61-39 214 5050 tel;home:+61-39 857 4251 tel;work:+61-39 214 5083 x-mozilla-html:FALSE url:http://www.swin.edu.au/iris/bcm/index.htm org:Swinburne University;Industrial Research Institute Swinburne adr:;;PO Box 218;Hawthorn;Victoria;3122;Australia version:2.1 email;internet:dnicolau@swin.edu.au title:Associate Professor, Bioengineering x-mozilla-cpt:;26384 fn:Dan Nicolau end:vcard --------------F983A3686B7195136E93095D-- --- From owner-biophys@hgmp.mrc.ac.uk Mon May 8 13:54:40 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id E776617A5D; Mon, 8 May 2000 13:54:37 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id NAA19252 for ; Mon, 8 May 2000 13:54:34 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id NAA18296 for biophys-list@hgmp.mrc.ac.uk; Mon, 8 May 2000 13:54:33 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: Andrea X-Newsgroups: bionet.biophysics Subject: porous media in biophysics Date: Mon, 08 May 2000 14:37:53 +0200 Organization: a Lines: 17 Message-ID: <3916B520.E10C99C2@cuorerossoblu.com> Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: news.tudelft.nl 957790468 1239 130.161.168.86 (8 May 2000 12:54:28 GMT) X-Complaints-To: usenet@news.tudelft.nl X-Mailer: Mozilla 4.61 [en] (WinNT; I) X-Accept-Language: en To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk Hi everybody. I'm fascinated by the biophysics/biomechanics domain though it is not at all my background. Indeed I come from civil engineering, and now I'm doing a lot of physics (modelling, numerics and laboratory experiments) for my PhD project (frequency dependent transport phenomena in porous media). So I have to questions for you: 1) could you suggest some review papers related to the porous media in biomechanics/biophysics? 2) do you think it will be too difficult for me succesfully applying for postdoc positions in biophysics/biomechanics with my background? Thanks for your help Andrea From owner-biophys@hgmp.mrc.ac.uk Mon May 8 18:05:26 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 9825E17ADF; Mon, 8 May 2000 18:05:24 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id SAA15557 for ; Mon, 8 May 2000 18:05:18 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id SAA21436 for biophys-list@hgmp.mrc.ac.uk; Mon, 8 May 2000 18:05:17 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: mezine@pobox.upenn.edu (Igor Mezine) X-Newsgroups: bionet.biophysics Subject: Chaotropic effect Date: 8 May 2000 17:04:25 GMT Organization: University of Pennsylvania Lines: 8 Message-ID: <8f6s2p$soj$1@netnews.upenn.edu> X-Newsreader: TIN [version 1.2 PL2-upenn1.3] To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk Hello, I'm getting frustrated trying to find "comprehensive" description of chaotropic effect. Is there any quantitive theory explaining this effect? Is it possible to predict "sign" and magnitute of influence of a salt (or small organic molecule) on the structure of water? Thanks in advance for help Igor From owner-biophys@hgmp.mrc.ac.uk Mon May 8 18:45:07 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 4B3E617AA7; Mon, 8 May 2000 18:45:07 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id SAA19238 for ; Mon, 8 May 2000 18:45:05 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id SAA21905 for biophys-list@hgmp.mrc.ac.uk; Mon, 8 May 2000 18:45:03 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: "Phillip Shelton" X-Newsgroups: bionet.biophysics Subject: Brainbench Biotechnology Beta Tests - Come test your Biotechnology skills! 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All rights reserved From owner-biophys@hgmp.mrc.ac.uk Tue May 9 07:01:40 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id F3FE517A66; Tue, 9 May 2000 07:01:39 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id HAA18664 for ; Tue, 9 May 2000 07:01:38 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id HAA09334 for biophys-list@hgmp.mrc.ac.uk; Tue, 9 May 2000 07:01:36 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: findthesecrets@china.com X-Newsgroups: bionet.biophysics Subject: Adv: Internet Spy Date: 9 May 2000 07:01:35 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 164 Message-ID: <200005090613.CAA07299@calderracecourse.com> X-Trace: niobium.hgmp.mrc.ac.uk 957852096 9332 193.62.192.80 (9 May 2000 06:01:36 GMT) X-Complaints-To: news@net.bio.net X-Received: from calderracecourse.com (calderracecourse.com [38.245.105.2]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with SMTP id HAA18657 for ; Tue, 9 May 2000 07:01:32 +0100 (BST) X-Received: from china.com by calderracecourse.com (SMI-8.6/SMI-SVR4) id CAA07299; Tue, 9 May 2000 02:13:59 -0400 X-To: findthesecrets@china.com To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk NOTE: THIS IS AN ADVERTISEMENT ON THE GREAT INTERNET SPY SOFTWARE. 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All REMOVE requests AUTOMATICALLY honored upon receipt. mailto: requestgrnted@flashmail.com subject=REMOVE PLEASE understand that any effort to disrupt, close or block this REMOVE account can only result in difficulties for others wanting to be removed from our mailing list as it will be impossible to take anyone off the list if the remove instruction is not received. ******************************************************* --- From owner-biophys@hgmp.mrc.ac.uk Tue May 9 12:05:41 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id B928817AFE; Tue, 9 May 2000 12:05:40 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id MAA13774 for ; Tue, 9 May 2000 12:05:38 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id MAA13326 for biophys-list@hgmp.mrc.ac.uk; Tue, 9 May 2000 12:05:36 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: seo15@mail.first-minute.ch (jusiertw) X-Newsgroups: bionet.biophysics Subject: -II IMPORTANT II- Date: 9 May 2000 12:05:35 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 54 Message-ID: <200005091090QAA10542@injsuert.cma.de> X-Trace: niobium.hgmp.mrc.ac.uk 957870336 13323 193.62.192.80 (9 May 2000 11:05:36 GMT) X-Complaints-To: news@net.bio.net X-Received: from cma-bn-ms1.cma.lan (gatekeeper.cma.de [195.145.228.193]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id MAA13741; Tue, 9 May 2000 12:05:23 +0100 (BST) X-Received: from localhost (cma-bn-lx1.cma.lan [10.192.0.11]) by cma-bn-ms1.cma.lan with SMTP (Microsoft Exchange Internet Mail Service Version 5.5.2650.21) id KQLDY0P2; Tue, 9 May 2000 13:04:06 +0200 X-To: seo15@mail.first-minute.ch X-Comments: Authenticated sender is To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk Hey there, This is something you're definitely going to want to follow up on. 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It could very well be what you have been waiting for all your life! http://3521854193/pg/a10300.html Best regards, Success Systems To be deleted from this list, please email jhoe99@bigfoot.com and put "un-subscribe" as the subject line., .. e/ --- From owner-biophys@hgmp.mrc.ac.uk Tue May 9 15:07:46 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id DB8F317B25; Tue, 9 May 2000 15:07:45 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id PAA03041 for ; Tue, 9 May 2000 15:07:44 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id PAA15630 for biophys-list@hgmp.mrc.ac.uk; Tue, 9 May 2000 15:07:43 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: Petr Kuzmic X-Newsgroups: bionet.biophysics Subject: [ANN] Freeware for biochemical kinetics and equilibria Date: Tue, 09 May 2000 09:09:04 -0500 Organization: BioKin Ltd. [http://www.biokin.com] Lines: 108 Message-ID: <39181C00.F82A6B4F@biokin.com> Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: news.chorus.net 957881194 20373 216.165.150.162 (9 May 2000 14:06:34 GMT) X-Complaints-To: abuse@chorus.net X-Mailer: Mozilla 4.72 [en] (WinNT; U) X-Accept-Language: en To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk ___________________________________________________________ [Volume 4] B i o K i n N E W S [Number 1] May, 2000 ----------------------------------------------------------- Editor: Petr Kuzmic, Ph.D. Contents -------- 1. DynaFit version upgrade 2. Purpose of program DynaFit 3. Symbolic notation 4. Revised documentation 5. Bibliography 6. Download DynaFit 1. DynaFit version upgrade -------------------------- The freeware program DynaFit [P. Kuzmic (1996) Anal. Biochem. 237, 260-173] has been updated to version 3.21 and is now available for download from the BioKin website. 2. Purpose of program DynaFit ----------------------------- DynaFit is used for the simulation or statistical analysis (nonlinear least-squares regression) of chemical, biochemical, and biological data. Three types of data can be analyzed: (1) reaction progress in dependence on time, (2) initial reaction velocity in dependence on concentrations, and (3) composition at equilibrium in dependence on concentrations. 3. Symbolic Notation -------------------- The main advantage of program DynaFit is that the investigator may describe the chemical or biochemical system in terms of symbolic, stoichiometric equations. The program derives the underlying mathematical model automatically. For example, interaction of a "slow, tight" inhibitor of a dimeric enzyme (HIV protease) is described as follows: [mechanism] Monomer + Monomer <==> Enzyme : k1 k2 Enzyme + Substrate <==> ReactiveX : k3 k4 ReactiveX --> Enzyme + Product : k5 Enzyme + Inhibitor <==> Complex : k6 k7 The names of rate constants (k1, k3, ...) and reacting species ('Monomer', 'Enzyme', ...) are arbitrary. 4. Revised documentation ------------------------ The long awaited DynaFit "Scripting Manual" is now available in the Adobe PDF format. It is a 130-page document with indexes and illustrations, explaining in a systematic fashion the syntax of DynaFit script files. Also added to the website is a short visual "Getting Started" tutorial, showing the analysis of a representative data set screen-by-screen. * Links: http://www.biokin.com/dynafit/scripting/ http://www.biokin.com/dynafit/quickstart/ 5. Bibliography --------------- The hyperlinks below take you to several recent journal publications in which DynaFit has been cited. * "Microcomputer Applications in Biochemistry" Tsai S. (2000) J. Chem. Edu. 77(2), 219. http://jchemed.chem.wisc.edu/Journal/Issues/2000/feb/abs219.html * "GroEL/GroES promote dissociation/reassociation cycles of a heterodimeric intermediate during alpha(2)beta(2) protein assembly. Iterative annealing at the quaternary structure level" Wynn R.M. et al. (2000) J. Biol. Chem. 275(4), 2786. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10644743&dopt=Abstract * "Hydrophilic residues at the apical domain of GroEL contribute to GroES binding but attenuate polypeptide binding." Motojima F. et al. (2000) Biochem. Biophys. Res. Commun. 267(3),842. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10673379&dopt=Abstract * "Structural basis for selectivity of a small molecule, S1-binding, submicromolar inhibitor of urokinase-type plasminogen activator." Katz B.A. et al. (2000) Chem. Biol. 7(4), 299. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10779411&dopt=Abstract 6. Download DynaFit ----------------------- Download DynaFit 3.21 for Windows95/98/NT or for Macintosh from http://www.biokin.com/dynafit/. ___________________________________________________________ BioKin, Ltd. * Consulting & Software P.O. Box 8336 * Madison, WI 53708 (608) 256-4790 * (608) 256-1269 FAX info@biokin.com * http://www.biokin.com From owner-biophys@hgmp.mrc.ac.uk Tue May 9 16:01:04 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 0281C17A7B; Tue, 9 May 2000 16:01:02 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id QAA09510 for ; Tue, 9 May 2000 16:00:58 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id QAA16425 for biophys-list@hgmp.mrc.ac.uk; Tue, 9 May 2000 16:00:56 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: Oliver X-Newsgroups: bionet.biophysics Subject: pH-measurement in protein containing samples Date: Tue, 09 May 2000 17:02:07 +0200 Organization: Johannes Gutenberg-Universitaet Mainz, Germany Lines: 16 Message-ID: <3918286F.F42B67D9@biophysik.biologie.uni-mainz.de> Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Trace: bambi.zdv.Uni-Mainz.DE 957884452 7364 134.93.248.74 (9 May 2000 15:00:52 GMT) X-Complaints-To: usenet@mail.uni-mainz.de X-Mailer: Mozilla 4.7 [de] (Win95; I) X-Accept-Language: de To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk Hello, I try to use a common pH-electrode (Ingold) to measure pH changes upon oxygenation of hemocyanin of spiders. The problem is, that I have to use concentrations between 22 to 32 g/l. The protein is denaturated inside the diaphragm of the electrode after a short time. Does anybody use the Friscolyt-electrolyt (Mettler-Toledo) to solve that problem? I also don´t know how to change the electrolyte. It´s a mico-electrode and capillary forces prevent simple change by pipette. Thank you for any help, Oliver From owner-biophys@hgmp.mrc.ac.uk Tue May 9 17:11:24 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 324B917B0D; Tue, 9 May 2000 17:11:22 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id RAA19307 for ; Tue, 9 May 2000 17:11:20 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id RAA17350 for biophys-list@hgmp.mrc.ac.uk; Tue, 9 May 2000 17:11:19 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: Frank Fuerst X-Newsgroups: bionet.biophysics Subject: Re: [ANN] Freeware for biochemical kinetics and equilibria Date: Tue, 09 May 2000 18:11:28 +0200 Lines: 15 Message-ID: <8f9db4$a6uci$1@fu-berlin.de> References: <39181C00.F82A6B4F@biokin.com> Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: fu-berlin.de 957888677 10713490 141.89.201.37 (16 955) X-Mailer: Mozilla 4.51 [de]C-CCK-MCD QXW03200 (WinNT; I) X-Accept-Language: de,en To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk Petr Kuzmic wrote: > > > 1. DynaFit version upgrade > -------------------------- > The freeware program DynaFit [P. Kuzmic (1996) Anal. Biochem. 237, > 260-173] has been updated to version 3.21 and is now available for > download from the BioKin website. With DynaFit, is it possible to do analyze multiple datasets simultaneously, e.g. kinetic and equilibrium data on several connected reactions, or sequential reactions monitored by different signals, each containing information about more than one of the phases? Frank From owner-biophys@hgmp.mrc.ac.uk Tue May 9 17:46:30 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 80E0217AD4; Tue, 9 May 2000 17:46:29 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id RAA23439 for ; Tue, 9 May 2000 17:46:26 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id RAA17735 for biophys-list@hgmp.mrc.ac.uk; Tue, 9 May 2000 17:46:25 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: Petr Kuzmic X-Newsgroups: bionet.biophysics Subject: Re: [ANN] Freeware for biochemical kinetics and equilibria Date: Tue, 09 May 2000 11:48:29 -0500 Organization: BioKin Ltd. [http://www.biokin.com] Lines: 170 Message-ID: <3918415D.37CAB3FA@biokin.com> References: <39181C00.F82A6B4F@biokin.com> <8f9db4$a6uci$1@fu-berlin.de> Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: news.chorus.net 957890761 22870 216.165.150.162 (9 May 2000 16:46:01 GMT) X-Complaints-To: abuse@chorus.net X-Mailer: Mozilla 4.72 [en] (WinNT; U) X-Accept-Language: en To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk Frank Fuerst wrote: > > Petr Kuzmic wrote: > > > > > > 1. DynaFit version upgrade > > -------------------------- > > The freeware program DynaFit [P. Kuzmic (1996) Anal. Biochem. 237, > > 260-173] has been updated to version 3.21 and is now available for > > download from the BioKin website. > > With DynaFit, is it possible to do analyze multiple datasets > simultaneously, e.g. kinetic and equilibrium data on several connected > reactions, or sequential reactions monitored by different signals, > each containing information about more than one of the phases? There are some portions of your question that I don't fully understand, but I'll try to answer as best as I can: ----- "Is it possible to do analyze multiple datasets simultaneously": ----- The answer is 'yes'. DynaFit can analyze multiple datasets treated as one 'super-set' of data. Some datasets in this 'super-set' can have adjustable parameters associated with them that are *local*, or characteristic only for the given dataset. For example, each dataset in the 'super-set' can have its own baseline offset or instrument response constant. On the other hand, other adjustable parameters are treated as *global* to the entire 'super-set' of data, i.e., a collection of datasets. For example, rate constants always are treated as global parameters, characteristic for the entire super-set of datasets. Sometimes people talk about "global analysis" in this case. ----- "...kinetic and equilibrium data": ----- If you could give an example, that would be useful, but if I understand you correctly the answer is 'yes', DynaFit can treat kinetic *and* equilibrium data in the same problem. For example, a chemical and biochemical mixture [e.g., enzyme + reversible inhibitor] could be initially allowed to achieve its equilibrium composition, characterized by some (as yet unknown) *equilibrium* constants. Call this the "equilibrium phase" of the experiment. Then the biochemical mixture is perturbed by adding some other reactant [e.g., substrate] after which the composition changes over time. Call this the "kinetic phase" of the experiment. DynaFit can analyze this kind of "hybrid" (equilibrium + kinetic) experiment. ----- "... on several connected reactions, or sequential reactions": ----- Yes, you can have an arbitrary mechanism treated by DynaFit, as long as it does not include 'fractional order' steps. In other words, we can have first-order, second-order, or zero-order [constant influx or efflux] elementary steps. The most complicated and *realistic* mechanism, included in the program distribution, is the mechanism taken from the blood coagulation cascade. You can see it at the end of this message. ----- "... monitored by different signals": ----- Yes. You can analyze simultaneously several datasets that were obtained by monitoring e.g. at different wavelengths in a time-resolved spectroscopic experiment. Each of the experimental signals can be sensitive to the presence of any number of species, but of course if the experiment were to be meaningful, the molar response coefficients would have to be different for each "channel" or signal. A good example would be a diode array spectrophotometer observing at several different different wavelengths the change of absorbance over time. Hope this helps, -- Petr _____________________________________________________________________ Petr Kuzmic, Ph.D. * BioKin, Ltd. * Consulting & Software Development http://www.biokin.com * (608) 256-4790 * (608) 256-1269 FAX ;------------------------------------------------- ; Tissue factor pathway to thrombin. ; ; Reference: Jones & Mann, J. Biol. Chem. 269, 23367 (1994). ; ; Purpose: ; ; Assuming that factors IIa and mIIa could be observed ; (with response factors 1.0 and 1.2, respectively), show ; the maximum on the [IIa + mIIa] concentration curve. ; Also display concentrations of various intermediates, ; as indicated in the 'monitor' section below. ;------------------------------------------------- [task] data = progress task = simulate [mechanism] IX + TF.VIIa <==> IX.TF.VIIa : k6 k16 IX.TF.VIIa --> TF.VIIa + IXa : k11 X + TF.VIIa <==> X.TF.VIIa : k6 k17 X.TF.VIIa --> TF.VIIa + Xa : k12 X + VIIIa.IXa <==> X.VIIIa.IXa : k6 k18 X.VIIIa.IXa --> VIIIa.IXa + Xa : k13 IX + Xa --> Xa + IXa : k15 V + Xa --> Va + Xa : k1 VIII + Xa --> VIIIa + Xa : k3 V + IIa --> IIa + Va : k2 VIII + IIa --> VIIIa + IIa : k4 II + Va.Xa <==> II.Va.Xa : k6 k19 II.Va.Xa --> Va.Xa + mIIa : k14 mIIa + Va.Xa --> Va.Xa + IIa : k5 VIIIa + IXa <==> VIIIa.IXa : k7 k9 Va + Xa <==> Va.Xa : k8 k10 ; VIIIa.IXa --> : k20 [constants] ; all bimolecular rate constants are micromolar k1 = 20, k2 = 20, k3 = 10, k4 = 20, k5 = 10 k6 = 100, k7 = 10, k8 = 400, k15 = 0.1 k9 = 0.005, k10 = 0.4, k11 = 0.3, k12 = 1.15, k13 = 8.2 k14 = 32, k16 = 24, k17 = 44, k18 = 0.001 k19 = 70 ; k20 = 0.00000002 [concentrations] ; all concentrations are micromolar TF.VIIa = 0.000005 II = 1 V = 0.1, VIII = 0.1, IX = 0.1, X = 0.1 [responses] IIa = 1, mIIa = 1.2 [progress] ; remove semicolon from one of the following three lines ; monitor TF.VIIa, IXa ; monitor II, IIa, mIIa monitor Va, VIII, VIIIa, IX, X, Xa mesh from 0 to 300 step 1 error constant 0 file ./examples/simulate/data/thrombin.txt [output] directory ./examples/simulate/output/thrombin [end] From owner-biophys@hgmp.mrc.ac.uk Tue May 9 19:36:26 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id BBB1817A7B; Tue, 9 May 2000 19:36:25 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id TAA02873 for ; Tue, 9 May 2000 19:36:24 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id TAA19085 for biophys-list@hgmp.mrc.ac.uk; Tue, 9 May 2000 19:36:23 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: Frank Fuerst X-Newsgroups: bionet.biophysics Subject: Re: [ANN] Freeware for biochemical kinetics and equilibria Date: Tue, 09 May 2000 20:36:31 +0200 Lines: 15 Message-ID: <8f9lr3$abi2v$1@fu-berlin.de> References: <39181C00.F82A6B4F@biokin.com> <8f9db4$a6uci$1@fu-berlin.de> <3918415D.37CAB3FA@biokin.com> Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: fu-berlin.de 957897379 10864735 141.89.201.37 (16 955) X-Mailer: Mozilla 4.51 [de]C-CCK-MCD QXW03200 (WinNT; I) X-Accept-Language: de,en To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk Petr Kuzmic wrote: > > Frank Fuerst wrote: > > > There are some portions of your question that I don't fully understand, > but I'll try to answer as best as I can: You guessed just what I was trying to say, and, > > Hope this helps, yes, it helps, and I think we'll use Dynafit! Thanks, Frank From owner-biophys@hgmp.mrc.ac.uk Wed May 10 01:26:08 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 703D917A56; Wed, 10 May 2000 01:26:07 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id BAA02362 for ; Wed, 10 May 2000 01:26:05 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id BAA01881 for biophys-list@hgmp.mrc.ac.uk; Wed, 10 May 2000 01:26:04 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: Vladimir Smorodin X-Newsgroups: bionet.biophysics Subject: 3D Cell structure of Pseudomonas syringae Date: Tue, 09 May 2000 20:18:14 -0400 Organization: University of Western Ontario Lines: 42 Message-ID: <3918AAC6.91F42EC@julian.uwo.ca> Reply-To: V_Smorodin@hotmail.com Mime-Version: 1.0 Content-Type: multipart/mixed; boundary="------------F2C2DF6131FD33C52121C1DA" X-Trace: panther.uwo.ca 957917887 9798 129.100.24.90 (10 May 2000 00:18:07 GMT) X-Complaints-To: abuse@julian.uwo.ca X-Mailer: Mozilla 4.72 [en] (Win98; U) X-Accept-Language: en,ru,fr-FR,pt To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk This is a multi-part message in MIME format. --------------F2C2DF6131FD33C52121C1DA Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Dear Colleagues: Would you kindly help me to find any references on the structure of cells of Pseudomonas syringae? Namely I am interested in the 3-D "architecture" of their metabolic apparatus (including locations of ADT, ADP etc units). Thank you in advance. Sincerely, Dr.Vladimir Smorodin --------------F2C2DF6131FD33C52121C1DA Content-Type: text/x-vcard; charset=us-ascii; name="vsmorodi.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for Vladimir Smorodin Content-Disposition: attachment; filename="vsmorodi.vcf" begin:vcard n:Smorodin;Vladimir tel;fax:1-519-661-3032 tel;work:1-519- 661 2111, ext. 86861 x-mozilla-html:TRUE url:www.itqb.unl.pt\people\Vladimir Smorodin org:University of Western Ontario;Physics & Astronomy Bldg., Center for Chemical Physics (CCP) adr:;;CCP, Physics & Astronomy Bldg., University of Western Ontario ;London;Ontario;N6A 3K7;CANADA version:2.1 email;internet:V_Smorodin@hotmail.com title:Senior Research Fellow note;quoted-printable:I am interested in creative partnership in area: =0D=0A=0D=0A1) Aerosol Science &Technology ( Atmospheric Aerosol &=0D=0AWeather Modification, Climate Change, Environment,=0D=0AHealth=3B Heterogeneous Atmospheric Chemistry=3B =0D=0AHeterogeneous Nucleation on Mixed Aerosol Particles=3B=0D=0AMechanisms of Maritime Aerosol Generation=3B=0D=0ABioaerosols=3B Ice Nucleating Bacteria=3B Technology of =0D=0APrecipitation of Indoor and Outdoor Aersosols etc)=3B =0D=0A=0D=0A2) Colloid & Interface Science and its Technology=0D=0AApplications (Separation Technology, Microflotation etc.)=3B=0D=0A=0D=0A3) Biocolloidal Systems, Biophysics & Biotechnology=0D=0A(Phase Transitions in Phospholipid Membarnes=3B=0D=0AHydrophobic Interactions and Mechanisms of Cell=0D=0AFusions=3B Bioenergetics of Ice Nucleation Bacteria etc.)=0D=0A=0D=0ASee Web Page: www.itqb.unl.pt/people/VladimirSmorodin fn:Vladimir Smorodin end:vcard --------------F2C2DF6131FD33C52121C1DA-- From owner-biophys@hgmp.mrc.ac.uk Wed May 10 13:44:26 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 2393517A4E; Wed, 10 May 2000 13:44:25 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id NAA02041 for ; Wed, 10 May 2000 13:44:22 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id NAA10682 for biophys-list@hgmp.mrc.ac.uk; Wed, 10 May 2000 13:44:20 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: Piers Mahon X-Newsgroups: bionet.biophysics Subject: DUAl RATIO IMAGING Date: Wed, 10 May 2000 13:50:48 +0100 Organization: University of Cambridge, England Lines: 21 Message-ID: <39195B28.F69BD275@mole.bio.cam.ac.uk> Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.03 [en] (WinNT; I) To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk I am about to try quantitative ratio imaging on a TCS using CYAN and YELLOW fluorescent proteins with different sub-cellular location signals. I am expecting any positive results to be very subtle images differences (i.e. not blue and yellow blobs in different parts of the cell, but a cyan/yellow signal ratio that varies in sub-sections of the same blob). If you have any experience of CYAN FP/ YELLOW FP imaging, or related dual imaging, please get in touch. I'm particularly interested in advice in three areas: 1) selecting the right filter ranges to obtain the best bleed through / sensitivity compromise, 2) Indications of the random noise levels I can expect in my negative controls of dual transformants containing CYAN and YELLOW with the same location signals, 3) Suggestions for statistical tests that I can perform to see if ratio images with different signals on the two reporters are more variable than images captured using the same signal. Many thanks in advance, Piers Mahon From owner-biophys@hgmp.mrc.ac.uk Fri May 12 09:11:49 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 3EFCD17AB4; Fri, 12 May 2000 09:11:47 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id JAA14802 for ; Fri, 12 May 2000 09:11:44 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id JAA00206 for biophys-list@hgmp.mrc.ac.uk; Fri, 12 May 2000 09:11:42 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: Peter Nollert X-Newsgroups: bionet.biophysics Subject: sequence to structure software / web page Date: Thu, 11 May 2000 16:00:02 -0700 Organization: UCSF, ITS Lines: 39 Message-ID: <391B3B72.246E2CC@msg.ucsf.edu> Mime-Version: 1.0 Content-Type: multipart/mixed; boundary="------------19B41EEB2271AC7182294A73" X-Mailer: Mozilla 4.7 [en] (WinNT; I) X-Accept-Language: en To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk This is a multi-part message in MIME format. --------------19B41EEB2271AC7182294A73 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Looking for software (preferentially web-based), that I can feed with a PDB file (not available in databases) and a sequence and that outputs a PDB datafile of a structural alignment. I don't really mind the type of algorithm used. (threading etc) Thanks, Peter --------------19B41EEB2271AC7182294A73 Content-Type: text/x-vcard; charset=us-ascii; name="nollert.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for Peter Nollert Content-Disposition: attachment; filename="nollert.vcf" begin:vcard n:Nollert;Peter tel;fax:(415) 476 1902 tel;home:(415) 564 1638 tel;work:(415) 502 8663 x-mozilla-html:TRUE url:http://msg.ucsf.edu/~nollert/home.html org:UCSF;Biochemistry & Biophysics adr:;;513 Parnassus Ave;San Francisco;CA;94143-0448;USA version:2.1 email;internet:nollert@msg.ucsf.edu title:PhD x-mozilla-cpt:;-21904 fn:Peter Nollert end:vcard --------------19B41EEB2271AC7182294A73-- From owner-biophys@hgmp.mrc.ac.uk Sun May 14 13:51:47 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 4819917A81; Sun, 14 May 2000 13:51:46 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id NAA24023 for ; Sun, 14 May 2000 13:51:44 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id NAA25002 for biophys-list@hgmp.mrc.ac.uk; Sun, 14 May 2000 13:51:43 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: congreso2001@cnoo.es (Congreso 2001) X-Newsgroups: bionet.biophysics Subject: I Virtual Meeting of Optics and Optometry Date: 14 May 2000 13:51:42 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 654 Message-ID: <0e8b61535120e50CANCER@correo.recol.es> Mime-Version: 1.0 Content-Type: text/html Content-Transfer-Encoding: quoted-printable X-Trace: niobium.hgmp.mrc.ac.uk 958308703 25000 193.62.192.80 (14 May 2000 12:51:43 GMT) X-Complaints-To: news@net.bio.net X-Received: from cns.bu.edu (CNS.BU.EDU [128.197.61.100]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id NAA23997; Sun, 14 May 2000 13:51:38 +0100 (BST) X-Received: from correo.recol.es (cancer.recol.es [193.149.2.141]) by cns.bu.edu ((8.8.8.buoit.v1.0)/8.8.8/(BU-S-10/08/98-v1.0)) with ESMTP id IAA00575 for ; Sun, 14 May 2000 08:49:55 -0400 (EDT) X-Received: from charlypc ([195.53.185.121]) by correo.recol.es with Microsoft SMTPSVC(5.5.1877.117.11); Sun, 14 May 2000 14:35:15 +0200 X-To: Virtual Meeting X-Reply-To: congreso2001@cnoo.es X-Mailer: MultiMailer (3.1.0) To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk

I= Virtual Meeting of Optics and Optometry

A= project, a reality
www.cnoo.es

National College of Opticians= and Optometrists

Spain

=09Internet offers more and more new interesting facets and= applications for professional activity. One of them is no doubt= the possibility of on-line meetings. The National College of= Opticians and Optometrists is going to join this type of= initiatives organizing a virtual meeting for the year 2001. The= access will be free for all of those people who fill out a= simple registration form, where a users name and a personal= entry password to the web will be given.

Why a virtual meeting?

=09Because we can break geographic= barriers. The= seat for the 2001 Meeting will be the web itself, therefore= every PC or Mac user will be able to access from any part of the= world.

=09Because multipresence provides us a= greater professional and cultural wealth:=

- Cooperating in the scientific= discipline progress: Contact with= professionals from other countries assures a greater= collaboration for the development of new techniques and = scientific routines.
- Learning from the experience of= somebody else: All of the= professional contributions related to visual health= can help us develop and better understand certain aspects of = optometry in those topics of our interest.

=09Because comfort and easy access= stimulate collaboration and participation.

- Since we can virtually go into it= and travel through the meeting from home, without
journeys nor specific program hours.
- Because it is free, with no additional costs.

Appointment in 2001

=09Emblematic date for fonds of science= fiction since it makes an allusion to a "cult" film

- After the 2000 Meeting and Academy= 2000: The virtual congress does not= pretend to shadow the habitual events. It must be considered= only as a complement for those professionals who usually= assiduously assist and as a clear opportunity for those who did= not make it because of several causes.
- First virtual meeting of the XXI century,= the century of great technologies: The= National College of Opticians and Optometrists makes a great bet= for the future, going into the technological routines that come= up.

Objectives

=09To promote an environment of= communication and relation between vision= professionals: The meeting is not only based on paper= presentations, since the main objective is to promote the= interchange of information by means of electronic mail, chats= and discussion forums.

=09To promote continuos education through= already technologically possible events: = It is currently possible to present information= through internet using all of the multimedia resources= (hypertext, graphics, photographs, audio and video).

Activities

A virtual meeting can offer almost the= same structure than a normal live meeting. On one hand we will= have collaborations, participation and exhibitions of scientific= photography that will group the different presented papers= depending on origin and used format and on the other hand we= will have communication between participants and signed up= people.

=09Collaborations: All of the events are= given by professionals of known prestige.

=09Conferences

=09Mini-courses

=09Round tables

=09Participation:= Depending on presentation format and used= resources, papers will be classified into the following:

=09Free communications (only text= format)

=09Electronic posters (text + photography or graphics)

=09Lectures (audio, text, photography or/and video= format)

=09Communication: All of the= presentations and papers will have a communication channel with= their authors.

=09Electronic mail (free communications and= posters)

=09Debate forums (papers and conferences)

=09Chat (about topics of interest)

=09Distribution list (all of the events of the= meeting): Subscription to this list will be= automatic after signing up for the meeting, this way all of the= registered people will periodically receive all of the= information about the development of the meeting through their= electronic mail.

=09Exhibition:

=09Scientific photography: Another possibility= for participating in the meeting is to collaborate in this= proposal sending photographs related with optometry and ocular= pathology. All of the illustrations will be permanently= exhibited in a virtual room attached to the meeting.

Conditions for sending summaries and= papers

Summaries

Summaries must be sent to the following address= congreso2001@cnoo.es including in the "subject" field the next= reference "summary for 2001= meeting" before the 15th of September of 2000. It must contain the next points:

=09Title of the Communication: in capital letters.= Short and indicative of the nature of the research.

=09Authors: following the title,= leaving one line space, write the name of the main author and= his abbreviated curriculum and then the co-authors. Use capital= letters and lower cases. Underline the name of the speaker in= the case of audio or video for the presentation.

=09Institution and locality: where the paper was= done.

=09Structure of the summary: it will not exceed= (250) two hundred and fifty words. The following aspects must be= included on the text:

=09Topic of the communication or objectives of= the study.

=09Material and/or methodology of the paper.

=09Proposals or results.

=09Conclusions

=09Key words, between 3 and 6 words for the index of the= article.

=09Contacting e-mail: For us to be able to= communicate the admission of the papers in the case of being= accepted.

=09Presentation format: There are four= possible ways of presenting the paper and it is essential to= point out which one of them will be chose for its= exhibition.

=09Free communications: Only text= format.

=09Electronic posters