From owner-biophys@hgmp.mrc.ac.uk  Fri Jun  2 20:49:51 2000
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From: karthi@uic.edu ("Dr. Karthikeyan Narayanan")
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Subject: in vitro nucleation test
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Hi, I want to study the crystal growth kinetics. Can anyone give me some
ideas?

--
********************************************************************************************

Narayanan Karthikeyan Ph.D.,
College of Dentistry,
Department of Oral Biology,
University of Illinois at Chicago
Chicago IL 60612
Tel: 312-413-5375
Fax: 312-413-6694
********************************************************************************************




---




From owner-biophys@hgmp.mrc.ac.uk  Mon Jun  5 06:10:03 2000
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From: jones999@earthlink.net
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From owner-biophys@hgmp.mrc.ac.uk  Tue Jun  6 03:16:52 2000
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Some years back, when I was working at the Harvard Science Center, I ran
across a reprint of what seemed to be a classic paper from the 40's or 50's
on why phosphate ion had the ideal characteristics for moving energy around
in living systems of the sort we have on Earth.  Since I can't recall the
author or title, searching for the paper has proven impossible, and I was
hoping someone here would point me in the right direction, as I promised a
science fiction author who likes to deal with extra-terrestrial chemistry
and biology that I'd send him a copy.  Thanks.





From owner-biophys@hgmp.mrc.ac.uk  Wed Jun  7 17:15:12 2000
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From: Siddhartha S Mitra <ssmitra@acsu.buffalo.edu>
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Subject: require constitutively active forms of Ras, Raf-1
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Can anyone tell me if you have the constitutively active  form of Ras,
Raf-1, JNK constructs in a vector form. This is extremely important for
my work.

Sincerely
Siddhartha S Mitra
Dept of Biological Sciences
Cooke Hall
SUNY at Buffalo
Buffalo NY 14260




From owner-biophys@hgmp.mrc.ac.uk  Fri Jun  9 06:53:54 2000
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From: skytop@wickedmail.com
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Subject: Master Disc 2000
Date: 9 Jun 2000 06:53:50 +0100
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---




From owner-biophys@hgmp.mrc.ac.uk  Sat Jun 10 19:42:17 2000
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From: Dong Xu <xud@ornl.gov>
X-Newsgroups: bionet.biophysics
Subject: Employment at Oak Ridge National Laboratory
Date: Sat, 10 Jun 2000 14:57:01 -0400
Organization: Oak Ridge National Lab, Oak Ridge, TN
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Employment at Oak Ridge National Laboratory Postdoctoral Research
Associates and Postmaster Software Engineer Positions in Computational
Biology/Bioinformatics

Applications are invited for postdoctoral and postmaster positions in
the Computational Biosciences Section of Life Sciences Division, at the
Oak Ridge National Laboratory. The successful candidates will
participate in research and development of computational algorithms and
software for mining microarray expression data, modeling biological
pathways, or protein structure prediction/modeling.

An advanced degree with demonstrated experience in computer programming
is required. Additional experience is desired in any of the following
areas: bioinformatics, computational biology, biological pathway
studies, protein folding/threading, high-performance computing,
large-scale software development. Oak Ridge National Laboratory
maintains well-equipped facilities; more information on the laboratory
and its divisions can be found at: http://www.ornl.gov and
http://compbio.lsd.ornl.gov/.

Funding is available for appointments of up to three years.  They are
offered through the ORNL Postdoctoral Research Associates Program and,
and the ORNL Postmasters Research Participation Program which are
jointly administered by ORNL's Office of University and Science
Education and the Oak Ridge Institute for Science and Education. These
programs are open to all qualified U.S. and non-U.S. citizens without
regard to race, color, age, religion, sex, national origin, physical or
mental disability, or status as a Vietnam-era veteran or disabled
veteran.

Interested candidates should complete and submit a "Recruited Position"
application for either the ORNL Postdoctoral Research Associates
Program, or the ORNL Postmasters Research Participation Program.  An
application and a complete description of these programs are available
at: http://www.orau.gov/orise/edu/postgrad/ornlpdoc.htm. or
http://www.orau.gov/orise/edu/postgrad/ornlpmappmat.htm.  A copy of the
application can also be obtained by contacting Bonnie DeJarnette at
865-241-5386, or dejarneb@orau.gov.   Specific technical questions
related to this position should be directed to: Ying Xu, Ph.D., Oak
Ridge National Laboratory, PO Box 2008, 1060 Commerce Park Drive, Oak
Ridge, TN 37831-6480, 865-574-7263; xyn@ornl.gov.

Please reference project #ORNL00-12 when applying for these positions.
Applications will be accepted until the positions are filled.





From owner-biophys@hgmp.mrc.ac.uk  Sun Jun 11 14:14:09 2000
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Subject: Poll - is science grossly underfunded?
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Hi there,

I just wondered if you'd like to give your views on funding in
science - in the form of a quick poll I'm carrying out on my
website. The question and options are:

Do you think science is grossly underfunded?
* Yes - they do good work and should get more cash
* Yes - some areas are, while others get all the money
* No - they don't do anything worthwhile anyway
* No - they should raise money from private investment

So far, almost 90% of voters have selected one of the first two
options. Do you agree? If you'd like to vote, go to:

http://www.voteserve.com/vs/main?p_dsn_id=278&p_flr_id=8#278

If you like this question, check out more science polls in the
Science section - including Biology, Physics and Medicine. If
you think you can do better, why not register to add your own
science questions to my site? I'm always looking for good
science polls!

Thanks for your time,  voteserve


--------------------------------------
http://www.voteserve.com/
The online voting and discussion forum 
where members can earn shares
* Sent from RemarQ http://www.remarq.com The Internet's Discussion Network *
The fastest and easiest way to search and participate in Usenet - Free!




From owner-biophys@hgmp.mrc.ac.uk  Mon Jun 12 15:55:11 2000
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From owner-biophys@hgmp.mrc.ac.uk  Mon Jun 12 17:06:05 2000
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From owner-biophys@hgmp.mrc.ac.uk  Tue Jun 13 09:20:13 2000
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From owner-biophys@hgmp.mrc.ac.uk  Tue Jun 13 14:07:11 2000
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From owner-biophys@hgmp.mrc.ac.uk  Wed Jun 14 22:29:31 2000
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Hello all,

I am studying the redox properties of my enzyme in GSH/GSSG and
cysteine/cystine buffers.  Does anyone have a method (presumably
published) for deoxygenating redox buffers and ensuring that they don't
become oxidised during an assay.  In most of the articles that I have
found in the literature, the authors failto describe any precautions
that they undertook to prevent oxidation.  Presumably atmospheric oxygen
and perhaps dissolved oxygen would affect these redox buffers.

I have one further question, the active site cysteines of my enzyme
become oxidised upon isolation.  How could I test whether these
cysteines have been converted into sulfinic or sulfenic acids?

Thank you for your time.  Any ideas would be appreciated.

Sincerely,
Che Pillay

Dept. of Biochemistry
School of Molecular and Cellular Biosciences
University of Natal
South Africa
pillayche@nu.ac.za


---




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From owner-biophys@hgmp.mrc.ac.uk  Thu Jun 15 09:28:05 2000
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From: Frank Fuerst <fant.1@gmx.net>
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Subject: Re: redox novice
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defaultuser@domain.com wrote:
> 
> I am studying the redox properties of my enzyme in GSH/GSSG and
> cysteine/cystine buffers.  Does anyone have a method (presumably
> published) for deoxygenating redox buffers and ensuring that they don't
> become oxidised during an assay.  In most of the articles that I have
> found in the literature, the authors failto describe any precautions
> that they undertook to prevent oxidation.  Presumably atmospheric oxygen
> and perhaps dissolved oxygen would affect these redox buffers.

We used to
1. Degass buffers (stir under vacuum)
2. Extensively let nitrogen bubble through the stirred solutions
3. Add redox reagents
4. And again let N2 bubble through at each handling step, using a
glass capillary.

When working in Eppendorf tubes, we even used argon because it's
heavier than air and will stay in the cups while opening: We had to
pre-incubate buffers for thermostating purposes, then add some
denatured protein. After this I didn't want nitrogen bubbles in the
solution which could possibly interfere with renaturation, so I used
argon for "bubbling" with the buffers, and after adding protein I only
"refilled" the gas space over the solution with argon.

HTH, Frank Fuerst

-- 
Department of physical biochemistry, Potsdam University




From owner-biophys@hgmp.mrc.ac.uk  Thu Jun 15 09:32:56 2000
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From: Frank Fuerst <fant.1@gmx.net>
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Subject: Testing cysteine content (was: redox novice)
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defaultuser@domain.com schrieb:
 
One other thing:
> 
> I have one further question, the active site cysteines of my enzyme
> become oxidised upon isolation.  How could I test whether these
> cysteines have been converted into sulfinic or sulfenic acids?

Ellman's Test works quite well for quantifying cysteine content in
proteins. But I'm not sure about the chemistry, so you should check
wether sulfinic or sulfenic acid would interfere with the reaction.

Frank
-- 
Die Verwendung von mehreren Ausrufezeichen macht die Aussage nicht
ausrufender sondern ausufernder. [Michael Bauer in dnq]




From owner-biophys@hgmp.mrc.ac.uk  Sun Jun 18 13:03:32 2000
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From: Martti Mela <martti.mela@iki.fi>
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Subject: Systems Thinking
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Hello every one,

I'd like to hear your comments how to proceed with the idea explained below:

One of my friends has written an English manuscript of Systems Thinking for
university level students and researches. The manuscript handles several
fields of science, such as electrical, fluidic, mechanical, thermal and
diffusional systems and tries to find similarities between these topics using
physical and mathematical approach.
The Finnish version of the Systems Thinking book is already in use in some
Finnish universities and because of the good reception he thought that it
would be interesting to publish the English version of the book in Internet so
that it would be licensed like Open Source software, under some kind of GNU
General Public License for documents.
In this case the development of the manuscript could be done on the Net by
researchers and graduate level students who study topics handled in the
manuscript.
Because licensing under non-profit license, the writing would be non-profit
(same with the original author), but every one who extends the manuscript
would also be the co-writer of the book.

There already exists this kind of development facilities for software, like
CVS, but I don't know what's the situation with creating documents. So, how to
license the manuscript so that it would be freely distributable an extendable
but the authors would have legal rights for it? 



Best Regards,

Martti M. Mela, M. Sc. (Eng) student, Electrical Engineering


[martti.mela@iki.fi, http://www.iki.fi/mmela/]




From owner-biophys@hgmp.mrc.ac.uk  Sun Jun 18 16:43:51 2000
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From owner-biophys@hgmp.mrc.ac.uk  Wed Jun 21 10:33:00 2000
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From: silvano.traverso@tilgher.it (Silvano Traverso)
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In the latest issue of Rivista di Biologia / Biology Forum, the 
international journal for theoretical biology founded in 1919, a
selection of abstracts from the 2nd International A. Gurwitsch
Conference is published. The Conference was held in Moscow, Russia, in
September, 1999 and was entitled: 'Non-equilibrium and coherent systems
in Biology, Biophysics and Biotechnology'.

Information about the journal (including tables of contents, abstracts
and articles) is available at the following address:
http://www.tilgher.it/biologiae.html

Best regards,

Silvano Traverso
Managing Editor
Rivista di Biologia / Biology Forum
http://www.tilgher.it/biologiae.html
silvano.traverso@tilgher.it


---




From owner-biophys@hgmp.mrc.ac.uk  Fri Jun 23 16:07:09 2000
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From: raman@bragg.bio.uci.edu (CS Raman)
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POSTDOCTORAL POSITIONS IN STRUCTURAL BIOLOGY

Two positions are available in the newly established
Structural Biology Center at the University of Texas.
Highly motivated individuals are sought to pursue
research in an active and collaborative environment.
One position will focus on x-ray crystallographic
studies of the proteins involved in the nitric oxide
signaling pathway (Cell 95: 939-950 [1998]).  
Another position is geared towards solving structures
of novel proteins in transcriptional silencing.
Diffraction-quality crystals and/or optimized protein
expression systems are in hand to provide a head-start 
on these projects. Both projects also present the
opportunity for pursuing biochemical and functional
studies in parallel with the structural analysis.  
The laboratory is equipped with state-of-the-art facilites
including a Rigaku generator, RAXIS IV++ detectors, Osmic
mirrors, cryo-cooling, and equipment for all aspects
of protein purification, expression, and characterization.

The applicant should have a Ph.D. in Biochemistry or 
Molecular Biophysics.  Preference will be given to those
candidates with training in macromolecular crystallography.
However, those with research expertise in protein chemistry,
molecular biology, or computational chemistry/biology are
encouraged to apply.  Interested candidates should send their
CV along with e-mail addresses of three references to 
craman@uci.edu OR Dr. C. S. Raman, Structural Biology Center,
Dept. of Biochemistry and Molecular Biology, University
of Texas - Houston Medical School, Suite 6.200, 6431 Fannin St,
Houston, TX 77030.  URL:deanweb.med.uth.tmc.edu.  The University
of Texas - Medical School in Houston is an Equal Employment/
Opportunity Employer.


---




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		 Postdoctoral Positions
		Structural Biology Center
	University of Texas-Houston Medical School

Two postdoctoral positions are available immediately to solve
protein structures using modern NMR methods.  Research focus is to 
elucidate the molecular basis for cell cycle regulation.  One project
involves studies on a novel protein that regulates oocyte 
maturation; another involves investigating a kinase that plays a key
role in cell proliferation.  Fellows will study the solution structures
and dynamics of proteins or protein fragments using hetero- and
homonuclear multidimensional gradient NMR techniques on a 600 MHz Avance
NMR spectrometer.  The projects will also incorporate proteomics
approaches to identify and characterize interacting proteins.

Candidates who are competent in macromolecular NMR methods and
solving structures or with a strong background in pulse program
development and interest in learning to solve structures are preferred.
Interested candidates should e-mail their CV with the names, addresses,
and e-mail of three references to sudha@scripps.edu or mail to

Dr. Sudha Veeraraghavan
Structural Biology Center 
Department of Biochemistry and Molecular Biology
University of  Texas-Houston Medical School
6431 Fannin Street
Houston, TX 77030

***The UT-Houston Medical School is an Equal Opportunity/Affirmative
Action Employer ***


!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
*************************************************************************


Sudha Veeraraghavan, Ph.D.              E-Mail: sudha@scripps.edu
Department of Molecular Biology TPC15
The Scripps Research Institute          Office: (858) 784-8824
10550 N. Torrey Pines Rd.		Harper Lab: (858) 784-9858
La Jolla, CA 92037                      FAX   : (858) 784-8895
							  ^^^^   

**************************************************************************
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!





---




From owner-biophys@hgmp.mrc.ac.uk  Tue Jun 27 01:35:21 2000
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Subject: Re: Phosphate ion -- uniqurely suited for energy transport?
Date: 26 Jun 2000 17:20:58 -0700
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>>>>> "Ed" == Ed Koenig <edko@shore.net> writes:

    Ed> Some years back, when I was working at the Harvard Science
    Ed> Center, I ran across a reprint of what seemed to be a classic
    Ed> paper from the 40's or 50's on why phosphate ion had the ideal
    Ed> characteristics for moving energy around in living systems of
    Ed> the sort we have on Earth.  Since I can't recall the author or
    Ed> title, searching for the paper has proven impossible, and I
    Ed> was hoping someone here would point me in the right direction,
    Ed> as I promised a science fiction author who likes to deal with
    Ed> extra-terrestrial chemistry and biology that I'd send him a
    Ed> copy.  Thanks.

This isn't as old as you say, and may not be the one, but it might
have citations that would help.

@Article{Westheimer87,
  author = 	 {Westheimer, F. H.},
  title = 	 {Why Nature Chose Phosphates},
  journal = 	 {Science},
  year = 	 1987,
  volume =	 235,
  pages =	 {1173--1178}
}




From owner-biophys@hgmp.mrc.ac.uk  Wed Jun 28 00:41:29 2000
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Dear  Sir/Madam;

As one of editors-in-chief of the international series in Advances in Fuzzy 
Mathematics and Engineering, published by Beijing Normal University Press , 
I am glad to tell you that we have printed
(1) A new volume collected Zadeh's papers, entitled Fuzzy Sets and Fuzzy  
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---




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From owner-biophys@hgmp.mrc.ac.uk  Fri Jun 30 16:44:52 2000
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From: "jk" <kaczorek@polnet.cc>
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Subject: IM LOOKING FOR MOLECULAR DYNAMICS TYPHOON 8600 - USERS WORLDWIDE
Date: Fri, 30 Jun 2000 17:47:02 +0200
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I am interested to buy TYPHOON 8600 and I am looking for users opinion.
If you use this equipment please contact to my e-mail.

jk








