From owner-biophys@hgmp.mrc.ac.uk Wed Nov 1 07:37:14 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 1E30517B62; Wed, 1 Nov 2000 07:37:14 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 80ACE17A4E; Wed, 1 Nov 2000 07:37:12 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id E9DEA415DA for ; Wed, 1 Nov 2000 07:37:07 +0000 (GMT) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id HAA20170 for biophys-list@hgmp.mrc.ac.uk; Wed, 1 Nov 2000 07:37:07 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f Message-ID: <39FFC6E8.102A6D3B@mediaone.net> From: George Hammond X-Mailer: Mozilla 4.05 [en] (Win95; I) MIME-Version: 1.0 X-Newsgroups: bionet.biophysics Subject: Formation of Metaphase plate in Mitosis? X-Priority: 1 (Highest) Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 31 Date: Wed, 01 Nov 2000 07:31:49 GMT X-Complaints-To: abuse@mediaone.net X-Trace: typhoon.ne.mediaone.net 973063909 24.218.1.16 (Wed, 01 Nov 2000 02:31:49 EST) Organization: Road Runner To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk [Hammond] I am a physicist, not a biologist, so excuse my elementary views in this area. I understand that during metaphase in mitosis, that the chromosomes (DNA molecules) align themselves in the "metaphase plate" which is the future cleavage plane of the cell. Can anyone tell me some more details of this alignment? For instance: 1. Do the chromosomes lie in the plane, or perpendicular to the plane? 2. Are they randomly oriented in the plane, or do they all point in the same direction? 3. At what point do they become attached to the microtubules (spindles) so that the whole assembly forms a rigid mechanical structure? 4. Are the chromosomes arranged into diploid "pairs" during this alignment, or as 46 individual randomly positioned chromosomes, in the plane? 5. Any "geometrical" or "mechanical-configurational" observations would be of extreme interest. Thanks in advance, George Hammond -- BE SURE TO VISIT MY WEBSITE, BELOW: ----------------------------------------------------------- George Hammond, M.S. Physics Email: ghammond@mediaone.net Website: http://people.ne.mediaone.net/ghammond/index.html ----------------------------------------------------------- From owner-biophys@hgmp.mrc.ac.uk Wed Nov 1 14:42:49 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 7C1BD17B6C; Wed, 1 Nov 2000 14:42:48 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 5BFDC17A6F; Wed, 1 Nov 2000 14:42:44 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 12B40415E9 for ; Wed, 1 Nov 2000 14:41:09 +0000 (GMT) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id OAA25500 for biophys-list@hgmp.mrc.ac.uk; Wed, 1 Nov 2000 14:41:09 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f Message-ID: <3A00061F.3BD5AAB1@mediaone.net> From: George Hammond X-Mailer: Mozilla 4.05 [en] (Win95; I) MIME-Version: 1.0 X-Newsgroups: bionet.biophysics Subject: DNA versus Roux's experiment X-Priority: 1 (Highest) Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 42 Date: Wed, 01 Nov 2000 12:01:30 GMT X-Complaints-To: abuse@mediaone.net X-Trace: typhoon.ne.mediaone.net 973080090 24.218.1.16 (Wed, 01 Nov 2000 07:01:30 EST) Organization: Road Runner To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk [Hammond] (physicist) The discovery of HOX genes, as I understand it, has proven that there is an axial isomorphism between the axial gene sequence on a DNA strand, and the axial sequence of body segments in simple animals (insects). In other words, it has been demonstrated that one end of the DNA strand codes for the head of an insect, and the other end codes for the tail of the insect (roughly generalizing to be sure). Since both DNA molecules and animals, are also bilaterally symmetric.. this leads to the inevitable conjecture that there is a complete geometric isomorphism between the DNA molecule and the zoological body plan. What I want to know is this: Is there a simple way to test this idea? If one were to snip off one end of a DNA molecule for a fruit fly or something; could it possibly produce a fruit fly with no head or no tail? Or is such gross manipulation generally lethal to the point where nothing would be reproduced? This of course, would be the equivalent of a "Roux's experiment" in genetics. As you will recall, Roux, the Father of Embryology discovered that if you kill one of the cells in the two cell stage of a (mosaic) egg, you will get half an animal. Secondly, if it IS true that the DNA molecule is a "homunculus" of the human body, is it a reasonable conjecture that some where along the line, there must be a physical alignment of the DNA molecule with the human body (or what is equivalent, with the cell); most likely occurring during the metaphaseplate-chromosome alignment during mitosis? I mean, if there is an axial isomorphism of the HOX genes and the body segments; why else would such a mechanical isomorphism exist unless there were a "mechanical isomorphism" somewhere in the growth process itself? GH -- BE SURE TO VISIT MY WEBSITE, BELOW: ----------------------------------------------------------- George Hammond, M.S. Physics Email: ghammond@mediaone.net Website: http://people.ne.mediaone.net/ghammond/index.html ----------------------------------------------------------- From owner-biophys@hgmp.mrc.ac.uk Wed Nov 1 14:59:16 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id B775B17B6E; Wed, 1 Nov 2000 14:59:14 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id EFAAD17AE3; Wed, 1 Nov 2000 14:59:09 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 1B4BD415DD for ; Wed, 1 Nov 2000 14:58:18 +0000 (GMT) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id OAA25645 for biophys-list@hgmp.mrc.ac.uk; Wed, 1 Nov 2000 14:58:17 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: epand@fhs.csu.mcmaster.ca ("Richard M. Epand") X-Newsgroups: bionet.biophysics Subject: Membrane biophysics meeting Date: 1 Nov 2000 14:58:14 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 122 Message-ID: References: <3A00061F.3BD5AAB1@mediaone.net> Mime-Version: 1.0 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: 8bit X-Trace: niobium.hgmp.mrc.ac.uk 973090696 25642 193.62.192.80 (1 Nov 2000 14:58:16 GMT) X-Complaints-To: news@net.bio.net X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 62F0117AE3; Wed, 1 Nov 2000 14:58:09 +0000 (GMT) X-Received: from fhs.csu.McMaster.CA (fhs.csu.McMaster.CA [130.113.45.46]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id C7DA0415F1 for ; Wed, 1 Nov 2000 14:57:19 +0000 (GMT) X-Received: from pcepandr (pc-epandr-r.CSU.McMaster.CA [130.113.67.37]) by fhs.csu.McMaster.CA with SMTP (8.7.1/8.7.1) id JAA10991 for ; Wed, 1 Nov 2000 09:57:02 -0500 (EST) X-Reply-To: X-To: X-Priority: 3 (Normal) X-MSMail-Priority: Normal X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2910.0) X-In-Reply-To: <3A00061F.3BD5AAB1@mediaone.net> X-Importance: Normal X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2615.200 To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk I would like to call your attention to a meeting to be held in Boston on February 17th on the topic "The Role of Membranes in Cell Death". The programme for this meeting is: Membrane Structure and Assembly 12:00 – 6:00 PM, Room 304, Hynes Convention Centre Saturday, February 17, 2001 The Role of Membranes in Cell Death 12:00 Introduction Richard M. Epand McMaster University I. Cell membranes and Apoptosis Sarah Spiegel and Barry Lentz (Chairs) Georgetown University Medical Center (SS); Univ. of North Carolina (BL) 12:10 P.M. Mitochondrial Membranes in Apoptotic and Necrotic Cell Death John J. Lemasters University of North Carolina 12:30 P.M. Requirement for acidic sphingomyelinase in CD95/Fas/APO-1-induced death Richard N. Kolesnick Memorial Sloan-Kettering Cancer Center 12:50 P.M. Cellular Functions of Ceramide: Impact of Physicochemical Properties Paavo Kinunen and Juta M. Holopainen University of Helsinki 1:00 P.M. Sphingomyelin domain formation and ceramide signaling. The interface between physical and biological studies Alicia Alonso Universidad del País Vasco 1:10 – 1:30 General Discussion 1:30 – 2:00 Coffee Break II. Antimicrobial peptides Robert Lehrer and Michael Zasloff (Chairs) University of California, Los Angeles (RL); Magainin Corp. (MZ) 2:00 Lipid modulation of the properties of channel-forming peptides Olaf Sparre Andersen Weill Medical College of Cornell University 2:20 Why and how are peptide-lipid interactions utilized for host-defense? Katsumi Matsuzaki Kyoto University 2:40 The nature of transmembrane pores formed by antimicrobial peptides Huey W. Huang Rice University 3:00 Specific interaction of the lantibiotic nisin with Lipid II leads to highly efficient pore formation E.J. Breukink, B.B. Bonev, I. Wiedermann, H-G. Sahl, A. Watts and B. de Kruijff University of Utrecht (EJB, BK); Oxford University (BBB, AW); Univ. Bonn (IW, H-GS) 3:20 Peptide-Mediated Vesicle-Vesicle Contact: A Role in Antibacterial Action Yolanda Cajal and Mahendra Kumar Jain University of Barcelona (YC) and Univ. of Delaware (MKJ) 3:40 Beta-amino acid oligomers with magainin-like activity Sam Gellman University of Wisconsin Discussion of Antimicrobial Peptides Paul Axelsen and Hans Vogel (Chairs) University of Pennsylvania (PA); Univ. of Calgary (HV) 4:00 The Carpet Mechanism for Membrane Permeation by Antimicrobial Peptides: A Molecular Basis for Cell Selective Lytic Peptides Yechiel Shai Weizmann Institute of Science 4:10 The Role of Hydrophobic Interactions for the Specific Membrane Damaging Activities of Antimicrobial Peptides Karl Lohner Institut für Biophysik und Röntgenstrukturforschung 4:20 The Interaction of the antimicrobial peptide gramicidin S with lipid bilayer model membranes E.J. Prenner, R.N.A.H. Lewis and Ronald N. McElhaney University of Alberta 4:30 – 4:50 General Discussion 4:50 – 5:10 Subgroup Business meeting 5:10 – 6:00 Subgroup mixer We are grateful to Avanti Polar Lipids, Alabaster, AL and NPS Pharmaceuticals, Salt Lake City, UT for their financial support of this meeting. --- From owner-biophys@hgmp.mrc.ac.uk Wed Nov 1 20:00:05 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 06B2617A92; Wed, 1 Nov 2000 20:00:04 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id C95F117A76; Wed, 1 Nov 2000 20:00:02 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 463AB415E9 for ; Wed, 1 Nov 2000 19:59:56 +0000 (GMT) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id TAA29557 for biophys-list@hgmp.mrc.ac.uk; Wed, 1 Nov 2000 19:59:55 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: faramarz@cns.bu.edu ("Faramarz Valafar") X-Newsgroups: bionet.biophysics Subject: METMBS'2001 call for papers and technical session proposals Date: 1 Nov 2000 19:59:54 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 234 Message-ID: Mime-Version: 1.0 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: 7bit X-Trace: niobium.hgmp.mrc.ac.uk 973108795 29555 193.62.192.80 (1 Nov 2000 19:59:55 GMT) X-Complaints-To: news@net.bio.net X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id AFAF917A76; Wed, 1 Nov 2000 19:59:52 +0000 (GMT) X-Received: from cns.bu.edu (CNS.BU.EDU [128.197.61.100]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 4ECDB415DD; Wed, 1 Nov 2000 19:59:45 +0000 (GMT) X-Received: from rostam (rostam [128.197.61.217]) by cns.bu.edu ((8.9.3.buoit.v1.0)/8.9.3/(BU-W-05/17/2000-v1.1)) with SMTP id OAA01302 for ; Wed, 1 Nov 2000 14:55:12 -0500 (EST) X-To: X-Priority: 3 (Normal) X-MSMail-Priority: Normal X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2910.0) X-MimeOLE: Produced By Microsoft MimeOLE V5.50.4133.2400 X-Importance: Normal To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk The 2001 International Conference on Mathematics and Engineering Techniques in Medicine and Biological Sciences (METMBS'2001) http://www.cns.bu.edu/metmbs/ June 25 - 28, 2001 Monte Carlo Resort, Las Vegas, Nevada, USA Call for Papers Recent advances in computer technology have provided the tools and the environment to study, analyze, and better understand complex systems. This technological development has enabled researchers to collect and analyze massive amounts of data to a scale previously not possible. The impact of this technology is now being felt in the medical field and in the biological sciences. In recent years, research in interdisciplinary areas such as Bioinformatics and computer assisted medical decision-making has dramatically intensified. METMBS'2001 aims to provide a platform for researchers to present and discuss recent breakthroughs in this area. The METMBS'2001 Conference will be held concurrently (i.e., same location and dates) with a number of international conferences: International Conference on Parallel and Distributed Processing Techniques and Applications (PDPTA'2001), the International Conference on Imaging Science, Systems, and Technology (CISST'2001), International Conference on Internet computing (IC'2001), ... The last concurrent meeting of this group of conferences in year 2000 had research contributions from 46 countries (the events held in Las Vegas had over 1100 participants from all over the world.) It is hoped that METMBS'2001 will maintain its strong international flavor in this year's meeting as well. You are invited to submit a draft paper of about 4 pages and/or a proposal to organize a technical session (see below for submission information). All accepted papers will be published in the conference proceedings. THE NAMES OF TECHNICAL SESSION CHAIRS WILL APPEAR AS ASSOCIATE EDITORS ON THE COVER OF THE CONFERENCE PROCEEDINGS. SCOPE Topics of interest include, but are not limited to, the following: o Bioinformatics: This includes informatics techniques in genomics gene sequencing, gene pattern discovery, gene pattern-function studies, and other genomics related studies). o Data mining in medicine and biological sciences. o Pattern recognition in medicine and biological sciences. o Signal processing in medicine and biological sciences (e.g. biomedical signal processing, etc.) o Image processing in medicine and biological sciences (e.g. biomedical image processing, biomedical imaging, etc.) o Medical decision-making. o Medical Physics. o Biomedical Engineering. o Biomedical Electronics. o Biosignal interpretation. o Any application of computers in Medicine and biological sciences (protein structure-function analysis, drug and protein design, molecular modeling and simulation, etc.) o Application of information technology in biomedicine (e.g. medical database management, information retrieval and use of computers in hospitals) o Application of Computational Intelligence (artificial neural networks, fuzzy logic, and evolutionary computing) in medicine and biological sciences o Medical and bio-computing. o Computer-based medical systems (automation in medicine, etc.) o Recent history (1990-1999) of Mathematics and engineering techniques in medicine and biological sciences, and what to expect during the next decade (2000-2009); New horizons. Review articles) o Other aspects and applications relating to technological advancements in medicine and biological sciences. SUBMISSION OF PAPERS Prospective authors are invited to submit three copies of their draft paper (about 4 pages) to F. Valafar (address is given below) by March 1, 2001. E-mail and Fax submissions are also acceptable. The length of the Camera-Ready papers (if accepted) will be limited to 7 pages. Papers must not have been previously published or currently submitted for publication elsewhere. The first page of the draft paper should include: title of the paper, name, affiliation, postal address, E-mail address, telephone number, and Fax number for each author. The first page should also include the name of the author who will be presenting the paper (if accepted) and a maximum of 5 keywords. PROPOSAL FOR ORGANIZING TECHNICAL SESSIONS Each technical session will have at least 6 paper presentations. The session chairs will be responsible for all aspects of their sessions, including soliciting papers, reviewing, selecting, ... The names of session chairs will appear as Associate Editors in the conference proceedings. After the conference, some sessions will be considered for publication in appropriate journals as Special Issues with the session proposer as the Guest Editor of the journal. Proposals to organize technical sessions should include the following information: name and address (+ E-mail) of the proposer, title of session, a 100-word description of the topic of the session, and a short description on how the session will be advertised (in most cases, session proposers solicit papers from colleagues and researchers whose work is known to the session proposer). Mail your proposal to F. Valafar (address is given below); E-mail submissions are preferred. EVALUATION PROCESS Papers will be evaluated for originality, significance, clarity, and soundness. Two researchers in the topical area will referee each paper. The Camera-Ready papers will be reviewed by one person. PUBLICATION The conference proceedings will be published by CSREA Press (ISBN). The proceedings will be available at the conference. Some accepted papers will also be considered for journal publication (soon after the conference). ORGANIZERS/SPONSORS A number of university faculty members and their staff, in cooperation with the Monte Carlo Resort (Conference Division, Las Vegas), will be organizing the conference. The conference will be sponsored by Computer Science Research, Education, & Applications Press (CSREA: USA Federal EIN # 58-2171953) in cooperation with research centers, international associations, international research groups, and developers of high-performance machines and systems. The complete list of sponsors and co-sponsors will be available at a later time. The last conference's sponsors included: CSREA, the National Supercomputing Center for Energy and the Environment - DOE, The International Association for Mathematics and Computers in Simulation, The International Technology Institute (ITI), The Java High Performance Computing research group, World Scientific and Engineering Society, Sundance Digital Signal Processing Inc., the Computer Vision Research and Applications Tech., and more. LOCATION OF CONFERENCE The conference will be held in the Monte Carlo Resort Hotel, Las Vegas, Nevada, USA. This is a mega hotel with excellent conference facilities and over 3000 rooms. The hotel is minutes from the Las Vegas airport with free shuttles to and from the airport. This hotel has many vacation and recreational attractions, including: waterfalls, casino, spa, pools & kiddie pools, sunning decks, Easy River water ride, wave pool with cascades, lighted tennis courts, health spa (with workout equipment, whirlpool, sauna, ...), arcade virtual reality game rooms, nightly shows, snack bars, a number of restaurants, shopping area, bars, ... Many of these attractions are open 24 hours a day and most are suitable for families and children. The negotiated hotel's room rate for conference attendees is very reasonable ($79 + tax) per night (no extra charge for double occupancy) for the duration of the conference. The hotel is within walking distance from most other Las Vegas attractions (major shopping areas, recreational destinations, fine dining and night clubs, free street shows, and more). For the benefit of our international colleagues: the state of Nevada neighbors with the states of California, Oregon, Idaho, Utah, and Arizona. Las Vegas is only a few driving hours away from other major cities and attractions, including: Los Angeles, San Diego, Phoenix, the Grand Canyon, and more. EXHIBITION An exhibit is planned for the duration of the conference. We have reserved 20+ exhibit spaces. Interested parties should contact F. Valafar (address is given below). All exhibitors will be considered to be the co-sponsors of the conference. IMPORTANT DATES March 1, 2001 (Thursday): Draft papers (about 4 pages) due April 2, 2001 (Monday): Notification of acceptance May 1, 2001 (Tuesday): Camera-Ready papers & Prereg. due June 25 - 28, 2001: METMBS'2001 Conference Proposals to organize technical sessions should be submitted as soon as possible. All accepted papers are expected to be presented at the conference. MEMBERS OF PROGRAM & ORGANIZING COMMITTEES The Program Committee is currently being formed. Those interested in joining the Program Committee should e-mail F. Valafar (faramarz@cns.bu.edu) the following information: Name, affiliation and position, complete mailing address, e-mail address, tel/fax numbers, a short biography together with research interests. OTHER INFORMATION It is planned to add other related conferences and workshops to be held simultaneously (same location and dates) creating an international multiconference. Each conference will have it's own proceedings and technical/research sessions. CONFERENCE CONTACT: Faramarz Valafar Cognitive and Neural Systems Boston University 677 Beacon Street Boston, MA 02215 Tel: (617) 353-5134 Fax: (617) 353-7755 E-mail: Faramarz@cns.bu.edu --- From owner-biophys@hgmp.mrc.ac.uk Fri Nov 3 09:20:20 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 10C8117B67; Fri, 3 Nov 2000 09:20:19 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 93D5117B3B; Fri, 3 Nov 2000 09:20:18 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 9AEBF415DA for ; Fri, 3 Nov 2000 09:20:14 +0000 (GMT) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id JAA14184 for biophys-list@hgmp.mrc.ac.uk; Fri, 3 Nov 2000 09:20:14 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: zigoteau@my-deja.com X-Newsgroups: bionet.biophysics Subject: Help requested: Solvent accessibility areas Date: Fri, 03 Nov 2000 09:12:19 GMT Organization: Deja.com - Before you buy. Lines: 26 Message-ID: <8ttvhd$sc3$1@nnrp1.deja.com> X-Article-Creation-Date: Fri Nov 03 09:12:19 2000 GMT X-Http-User-Agent: Mozilla/4.75 [en] (Win95; U) X-Http-Proxy: 1.0 x59.deja.com:80 (Squid/1.1.22) for client 194.66.39.67 X-MyDeja-Info: XMYDJUIDzigoteau To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk Hi! Could anybody out there help me with a problem of protein analysis? I want to calculate the area of the surface of a given protein accessible to solvent. The protein is defined in a .pdb file or similar. Protein Explorer allows me to display the surface as a 2D graphic, so that this information must be accessible to it, but I do not seem to be able to find the button which would give me the answer in square angstroms. Perhaps I need to fork out real money to buy a proper program? Could anybody advise me which program does what I want? Best regards, Zigoteau. Sent via Deja.com http://www.deja.com/ Before you buy. From owner-biophys@hgmp.mrc.ac.uk Tue Nov 7 04:16:16 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id ACEBD17B42; Tue, 7 Nov 2000 04:16:15 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 2B21B17A6E; Tue, 7 Nov 2000 04:16:14 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 97A93415DC for ; Tue, 7 Nov 2000 04:16:09 +0000 (GMT) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id EAA23605 for biophys-list@hgmp.mrc.ac.uk; Tue, 7 Nov 2000 04:16:08 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: Axel Boldt X-Newsgroups: sci.chem.analytical,sci.physics,bionet.biophysics Subject: Electrophoresis: why mobility linear in log(weight)? Date: 07 Nov 2000 05:15:58 +0100 Organization: none Lines: 14 Message-ID: X-Trace: news.uni-paderborn.de 973570560 29097 131.234.38.3 (7 Nov 2000 04:16:00 GMT) X-Complaints-To: usenet@news.uni-paderborn.de X-Newsreader: Gnus v5.5/Emacs 20.3 To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk Hi, in gel electrophoresis of particles whose charges are proportional to their molecular masses and whose shapes are "long strings", the observed velocity depends roughly linearly on the logarithm of the molecular mass (if applied voltage, temperature, particle density, pH, gel concentration, ionic strength etc. are constant). Can anybody think of a model that would predict this relationship? Thanks, Axel -- Axel Boldt ** axel@uni-paderborn.de ** math-www.uni-paderborn.de/~axel/ Sponsor free software at the Free Software Bazaar visar.csustan.edu/bazaar/ From owner-biophys@hgmp.mrc.ac.uk Tue Nov 7 21:43:33 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 1211317B6D; Tue, 7 Nov 2000 21:43:33 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id D04F917B6B; Tue, 7 Nov 2000 21:43:31 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 53737415D4 for ; Tue, 7 Nov 2000 21:43:27 +0000 (GMT) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id VAA14867 for biophys-list@hgmp.mrc.ac.uk; Tue, 7 Nov 2000 21:43:26 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: Peter Lasch X-Newsgroups: bionet.biophysics Subject: Re: Help requested: Solvent accessibility areas Date: Tue, 07 Nov 2000 16:56:40 -0500 Organization: Hunter College, CUNY Lines: 48 Message-ID: <3A087A98.C0902153@peter-lasch.de> References: <8ttvhd$sc3$1@nnrp1.deja.com> Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: fu-berlin.de 973633404 1309589 146.95.6.113 (16 [21784]) X-Mailer: Mozilla 4.51 [en] (WinNT; I) X-Accept-Language: de,en To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk zigoteau@my-deja.com wrote: > > Hi! > > Could anybody out there help me with a problem of > protein analysis? I want to calculate the area of > the surface of a given protein accessible to solvent. > The protein is defined in a .pdb file or similar. > > Protein Explorer allows me to display the surface > as a 2D graphic, so that this information must be > accessible to it, but I do not seem to be able to > find the button which would give me the answer in > square angstroms. > > Perhaps I need to fork out real money to buy a > proper program? Could anybody advise me which program > does what I want? > > Best regards, > > Zigoteau. > > Sent via Deja.com http://www.deja.com/ > Before you buy. try the program 'NACCESS' Version 2.1 (Hubbard & Thornton, 1993) (I don't know if it is freeware or you have to buy it). Hope this helps Peter -- **************************************** Dr. Peter Lasch Department of Chemistry and Biochemistry City University of New York (CUNY) 695 Park Ave, New York 10021 NY Tel.: +1 212-772-5388 Fax.: +1 212-772-5332 e-mail:LaschP@hotmail.com http://www.peter-lasch.de **************************************** From owner-biophys@hgmp.mrc.ac.uk Thu Nov 9 09:51:47 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 499F517B83; Thu, 9 Nov 2000 09:51:43 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 8E59617B7D; Thu, 9 Nov 2000 09:51:38 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id E535B415DF; Thu, 9 Nov 2000 09:51:25 +0000 (GMT) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id JAA19548; Thu, 9 Nov 2000 09:51:25 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: "Sofie Ludwig" X-Newsgroups: aus.education.bio-newtech,bionet.biophysics,bionet.cellbiol,bionet.cellbiol.cytonet,bionet.general,bionet.info-theory,de.sci.biologie,sci.bio.misc,sci.bio.technology,sci.engr.biomed,z-netz.wissenschaft.biologie Subject: cell adhesion on coated coated Date: Thu, 9 Nov 2000 10:49:07 +0100 Organization: Humboldt Universitaet zu Berlin Lines: 11 Message-ID: <8udruf$fkv$1@lnews.rz.hu-berlin.de> Reply-To: "Sofie Ludwig" X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2615.200 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2615.200 To: bio-info@hgmp.mrc.ac.uk, bioforum@hgmp.mrc.ac.uk, biophys@hgmp.mrc.ac.uk, cellbiol@hgmp.mrc.ac.uk, cytonet@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk Hello I´m about to start some studies on cell adhesion on surfaces coated with different substances such as cell culture flasks. Does anyone has any ideas how I can test the stickiness (adherence forces) of the cells. Thanks Sofie From owner-biophys@hgmp.mrc.ac.uk Thu Nov 9 13:06:25 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id B599F17A65; Thu, 9 Nov 2000 13:06:24 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 1327417A89; Thu, 9 Nov 2000 13:06:18 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 0CB7941608 for ; Thu, 9 Nov 2000 12:54:42 +0000 (GMT) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id MAA21898 for biophys-list@hgmp.mrc.ac.uk; Thu, 9 Nov 2000 12:54:42 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: v_matveev@hotmail.com ("Vladimir Matveev") X-Newsgroups: bionet.biophysics Subject: Newest definition of a cellular signal Date: 9 Nov 2000 12:54:40 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 46 Message-ID: Mime-Version: 1.0 Content-Type: multipart/alternative; boundary="----=_NextPart_001_0004_01C04A65.396BEAE0" X-Trace: niobium.hgmp.mrc.ac.uk 973774482 21896 193.62.192.80 (9 Nov 2000 12:54:42 GMT) X-Complaints-To: news@net.bio.net X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 3B2D517A65; Thu, 9 Nov 2000 12:54:37 +0000 (GMT) X-Received: from hotmail.com (oe54.pav1.hotmail.com [64.4.30.47]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id F156541608 for ; Thu, 9 Nov 2000 12:54:07 +0000 (GMT) X-Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; Thu, 9 Nov 2000 04:54:06 -0800 X-Originating-IP: [194.85.224.35] X-To: X-Mailer: MSN Explorer 6.00.0010.0900 X-OriginalArrivalTime: 09 Nov 2000 12:54:06.0462 (UTC) FILETIME=[24F8A9E0:01C04A4C] To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk ------=_NextPart_001_0004_01C04A65.396BEAE0 Content-Type: text/plain; charset="koi8-r" Content-Transfer-Encoding: quoted-printable Newest definition of a cellular signal and the description of the protein= model of a cellular signal transduction see in the following paper: V.V.Matveev. Evidence of a new type of protein-protein interaction: desen= sitized actomyosin blocks Ca2+-sensitivity of the natural one. A possible= model for an intracellular signalling system related to actin filaments.= Physiological Chemistry Physics & Medical NMR, 32: 167-179, 2000. ABSTRACT see here: http://members.nbci.com/vm_spb/actomyosin/signal.htm V.Matveev v_matveev@hotmail.com____________________________________________________= _______ Get more from your time online. FREE MSN Explorer download : http://expl= orer.msn.com ------=_NextPart_001_0004_01C04A65.396BEAE0 Content-Type: text/html; charset="koi8-r" Content-Transfer-Encoding: quoted-printable

Newest= definition of a cellular signal and the description of the protein model= of a cellular signal transduction see in the following paper:

V.V.Matveev. Evidence of a new type of protein-protein= interaction: desensitized actomyosin blocks Ca2+-sensitivity of the natu= ral one. A possible model for an intracellular signalling system related = to actin filaments. Physiological Chemistry Physics & Medical NMR, 32= : 167-179, 2000.

ABSTRACT see here: http://members.nbc= i.com/vm_spb/actomyosin/signal.htm

V.Mat= veev
v_matveev@hotmail.com

_______________________________________= ____________________
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--------------FF7A5EEC25D05D0B73BC582A-- From owner-biophys@hgmp.mrc.ac.uk Wed Nov 22 19:07:16 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 0E5BE17B77; Wed, 22 Nov 2000 19:07:04 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 2F81C17B6B; Wed, 22 Nov 2000 19:06:52 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id ADC9A41602 for ; Wed, 22 Nov 2000 19:03:55 +0000 (GMT) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id TAA05416 for biophys-list@hgmp.mrc.ac.uk; Wed, 22 Nov 2000 19:03:55 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: hxyntn@hotmail.com X-Newsgroups: bionet.biophysics Subject: The Perfect Christmas Gift 8819 Lines: 7 Message-ID: X-Abuse-Info: Please be sure to forward a copy of ALL headers X-Abuse-Info: Otherwise we will be unable to process your complaint properly X-Complaints-To: abuse@webusenet.com Organization: WebUseNet Corp http://www.usenetserver.com - Home of the fastest NNTP servers on the Net. Date: Wed, 22 Nov 2000 17:02:43 GMT To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk Two days ago I ordered 10 pieces of paper money from 10 different countries from a company called Perth Numismatics. Lo and behold they arrived today and they are very nice and colourful. They even have a bill from Antarctica. I didn't even know they existed. These are perfect for stocking stuffers or people who are hard to buy for. The website address is www.perthmoney.com Good luck and Merry Christmas Cynthia Reeves vzyrsynsiehps From owner-biophys@hgmp.mrc.ac.uk Thu Nov 23 08:00:10 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 33FFA17ACF; Thu, 23 Nov 2000 08:00:10 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id EFB1917ACE; Thu, 23 Nov 2000 08:00:08 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 49B82415E5 for ; Thu, 23 Nov 2000 08:00:05 +0000 (GMT) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id IAA23166 for biophys-list@hgmp.mrc.ac.uk; Thu, 23 Nov 2000 08:00:04 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: Vladimir Matveev X-Newsgroups: bionet.biophysics Subject: New type of protein-protein interaction Date: Thu, 23 Nov 2000 07:48:56 GMT Organization: Deja.com - Before you buy. Lines: 14 Message-ID: <8vii58$vi6$1@nnrp1.deja.com> References: <3A00061F.3BD5AAB1@mediaone.net> X-Article-Creation-Date: Thu Nov 23 07:48:56 2000 GMT X-Http-User-Agent: Mozilla/4.0 (compatible; MSIE 5.5; Windows 98) X-Http-Proxy: 1.0 www-proxy.ioffe.rssi.ru:3128 (Squid/2.3.STABLE4), 1.0 x62.deja.com:80 (Squid/1.1.22) for client 194.85.234.70, 194.85.224.35 X-MyDeja-Info: XMYDJUIDvm_spb To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk New type of protein-protein interaction: actomyosin gels interact in a THRESHOLD manner. For details see the following paper: V.V.Matveev. Evidence of a new type of protein-protein interaction: desensitized actomyosin blocks Ca2+-sensitivity of the natural one. A possible model for an intracellular signalling system related to actin filaments. Physiological Chemistry Physics & Medical NMR, 32: 167-179, 2000. ABSTRACT see here: http://members.nbci.com/vm_spb/actomyosin/signal.htm Sent via Deja.com http://www.deja.com/ Before you buy. From owner-biophys@hgmp.mrc.ac.uk Fri Nov 24 13:02:43 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id A12AC17A72; Fri, 24 Nov 2000 13:02:42 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 5554C17A66; Fri, 24 Nov 2000 13:02:34 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 8C732415DD for ; Fri, 24 Nov 2000 13:02:06 +0000 (GMT) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id NAA22959 for biophys-list@hgmp.mrc.ac.uk; Fri, 24 Nov 2000 13:02:05 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: Kieran Gillick X-Newsgroups: bionet.biophysics Subject: Circular dichroism Date: Fri, 24 Nov 2000 13:01:27 +0000 Organization: University of Cambridge, England Lines: 32 Message-ID: <3A1E66A6.F374F966@cam.ac.uk> Mime-Version: 1.0 Content-Type: multipart/alternative; boundary="------------E8085FAF485DDA7005D656F8" X-Mailer: Mozilla 4.74 [en] (Win95; U) X-Accept-Language: en To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk --------------E8085FAF485DDA7005D656F8 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Does anyone know what a 'missing amplitude' is? I'm doing some work on protein folding pathways, and there's loads of stuff in the literature about 'missing amplitudes' in CD spectra indicating multi-stage kinetics/burst phases... Why does a burst phase give a missing amplitude, and what exactly is a missing amplitude? Any help would be much appreciated. If you could reply to kpg21@cam.ac.uk, that would be wicked. Many thanks, Kieran Gillick --------------E8085FAF485DDA7005D656F8 Content-Type: text/html; charset=us-ascii Content-Transfer-Encoding: 7bit Does anyone know what a 'missing amplitude' is? I'm doing some work on protein folding pathways, and there's loads of stuff in the literature about 'missing amplitudes' in CD spectra indicating multi-stage kinetics/burst phases... Why does a burst phase give a missing amplitude, and what exactly is a missing amplitude? Any help would be much appreciated. If you could reply to kpg21@cam.ac.uk, that would be wicked.
Many thanks,
Kieran Gillick --------------E8085FAF485DDA7005D656F8-- From owner-biophys@hgmp.mrc.ac.uk Fri Nov 24 15:01:57 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 0948217AE8; Fri, 24 Nov 2000 15:01:55 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 9D96417AE7; Fri, 24 Nov 2000 15:01:53 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id E789D415DE for ; Fri, 24 Nov 2000 15:01:38 +0000 (GMT) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id PAA24500 for biophys-list@hgmp.mrc.ac.uk; Fri, 24 Nov 2000 15:01:38 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: Frank Fuerst X-Newsgroups: bionet.biophysics Subject: Re: Circular dichroism Date: Fri, 24 Nov 2000 16:01:34 +0100 Lines: 70 Message-ID: <8vlvsd$4u70k$1@fu-berlin.de> References: <3A1E66A6.F374F966@cam.ac.uk> Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: fu-berlin.de 975078093 5184532 141.89.201.37 (16 49 955) X-Newsreader: Forte Agent 1.8/32.548 To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk Kieran Gillick wrote: >Does anyone know what a 'missing amplitude' is? I'm doing some work on >protein folding pathways, and there's loads of stuff in the literature >about 'missing amplitudes' in CD spectra indicating multi-stage >kinetics/burst phases... Why does a burst phase give a missing >amplitude, and what exactly is a missing amplitude? What you observe when recording any kinetics with a spectroscopic signal is always the difference between the signal of the products and the educts. For a protein folding kinetics, this means the difference of unfolded protein (at the beginning) and native protein (in the end). Let's assume the unfolded species have an ellipticity (CD-signal) of zero, which is sensible, and the native protein has an ellipticity of 1 (just to keep figures simple). Then you would expect to observe a kinetic trace starting at 0 and ending at 1, often with a monoexponential time course [Theta = 1-exp(-t/tau) = 1-exp(-kt)]. But no let's look what happens if there is an intermediate that has a signal between that of the native and that of the unfolded protein, say 0.5 (for far-UV-CD this would mean that the intermediate has about half of the structure formed[1]). Now we would first see a fast rise to nearly 0.5 that smoothly merges into a slower rise to unity. However, if the intermediate is formed very fast, its formation is completed in the dead-time of mixing, so we cannot see the fast rise to 0.5 - we simply start at 0.5 and see the slow rise from 0.5 to 1. And the range from 0 to 0.5 is the 'missing amplitude'. Generally, 'missing amplitude' means that the amplitude of the change of a spectroscopic signal is smaller than expected from the difference in amplitude of the native and denatured signal. To be able to conclude that there is a kinetic intermediate on the folding pathway present, one has to make sure, however, that this missing amplitude is really missing *in*the*folding*reaction*. Two main other sources, or better sinks ;-), of amplitude occur to me: 1. Refolding yield One should always compare the absolute end value of the kinetics with the signal of native protein at the same protein concentration, because if the refolding yield is below 100%, you don't see 100% of the amplitude change, of course. 2. Dependence of the signal of denatured protein on the concentration of denaturant. This is especially important for fluorescence: When one measures the fluorescence of denatured protein at different concentrations of denaturant, all high enough to be sure that it is completely unfolded, one usually gets different values, because the denaturant interacts with the solvent exposed fluorophores. (And the effect is even stronger with heat denaturation). Thus, to calculate the expected amplitude of a refolding reaction, one has to extrapolate these values to the denaturant concentration at which the refolding experiment is carried out. Of course this can also lead to an increase in refolding amplitude. I hope this clarifies the topic a little. Yours, Frank [1] or 3/4 of the secondary structure, but it's in fast equilibrium with unfolded forms and populated only 2/3 of the time, or similar numbers. -- Was W. Richard Stevens angeht: Auf dessen Buecher verweist man nicht einzeln, dessen Buecher sind eigentlich Pflichtlektuere, da schaeme ich mich eigentlich, wenn ich eines nicht auswendig kann. Und ich muss mich da sehr kraeftig schaemen. [Detlef Bosau in dcoun] From owner-biophys@hgmp.mrc.ac.uk Sat Nov 25 23:16:02 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id B9FD417AFB; Sat, 25 Nov 2000 23:16:01 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 6906B17AA4; Sat, 25 Nov 2000 23:16:00 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 7E5DA415CF for ; Sat, 25 Nov 2000 23:15:56 +0000 (GMT) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id XAA04797 for biophys-list@hgmp.mrc.ac.uk; Sat, 25 Nov 2000 23:15:55 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: suzya@dns.espar.co.jp X-Newsgroups: bionet.biophysics Subject: Pay Nothing -- Incredible Satellite Offer [yoqsx] Date: 25 Nov 2000 23:15:54 -0000 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 223 Message-ID: X-Trace: niobium.hgmp.mrc.ac.uk 975194154 4794 193.62.192.80 (25 Nov 2000 23:15:54 GMT) X-Complaints-To: news@net.bio.net X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 6C43117AA4; Sat, 25 Nov 2000 23:15:53 +0000 (GMT) X-Received: from outgoing.ns-center.net (outgoing.ns-center.net [206.112.2.241]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 4A8D9415CF for ; Sat, 25 Nov 2000 23:15:48 +0000 (GMT) X-Received: from stfrancis.ntr.net (stfrancis.ntr.net [206.115.84.124]) by outgoing.ns-center.net (8.9.3/8.9.3) with ESMTP id SAA26894; Sat, 25 Nov 2000 18:15:16 -0500 X-Received: from ST_FRANCIS/SpoolDir by stfrancis.ntr.net (Mercury 1.31); 25 Nov 100 17:58:19 -500 X-Received: from SpoolDir by ST_FRANCIS (Mercury 1.31); 25 Nov 100 13:25:26 -500 X-Received: from kujyy.dns.espar.co.jp by stfrancis.ntr.net (Mercury 1.31); 25 Nov 100 13:25:23 -500 X-To: wgwiu@deltacmas.deltacm.ru X-Reply-To: To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk You get a FREE Satellite Dish with Receiver... PLUS: => FREE Installation => FREE additional receiver => FREE 3 months of movies (optional) => Digital quality picture and CD sound Now Available for an "extremely" Limited Time. That's right! You