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From owner-biophys@hgmp.mrc.ac.uk  Tue Sep  4 04:39:33 2001
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From: mfireland@yahoo.com (Matthew Ireland)
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Subject: Perforated Patch Problem
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Dear Colleagues 

Currently I am using perforated patch on neurons within the CNS.
Recently, I have been experinceing a problem when I try to perforate a
neuron, instead of a full perforation as you would expect, it only
results in a partial perforation.

I have tried different concentrations of Amphotericin B, different
filling times and modified electrodes. All without much success.

I would appreciate any suggestions

With thanks,

Matthew Ireland
University of Queensland


From owner-biophys@hgmp.mrc.ac.uk  Tue Sep  4 10:39:49 2001
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Alhtough not likely, but could your ampho have gone nearly completely
gone bad?


On 3 Sep 2001 20:27:23 -0700, mfireland@yahoo.com (Matthew Ireland)
wrote:

>Dear Colleagues 
>
>Currently I am using perforated patch on neurons within the CNS.
>Recently, I have been experinceing a problem when I try to perforate a
>neuron, instead of a full perforation as you would expect, it only
>results in a partial perforation.
>
>I have tried different concentrations of Amphotericin B, different
>filling times and modified electrodes. All without much success.
>
>I would appreciate any suggestions
>
>With thanks,
>
>Matthew Ireland
>University of Queensland


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From owner-biophys@hgmp.mrc.ac.uk  Wed Sep  5 00:56:26 2001
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From: mfireland@yahoo.com (Matthew Ireland)
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justus.verhagen@psy.ox.ac.uk (Justus Verhagen) wrote in message news:<3b949d46.521116226@news.ox.ac.uk>...

Good question. But the amphotericin is relatively new and I checked
with our ordering company about the shelf life and they said that it
normally lasts for 3 years.

Thanks anyway,
Matthew


From owner-biophys@hgmp.mrc.ac.uk  Sat Sep  8 16:09:47 2001
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On 3 Sep 2001, Matthew Ireland wrote:

   > Dear Colleagues
   >
   > Currently I am using perforated patch on neurons within the CNS.
   > Recently, I have been experinceing a problem when I try to perforate a
   > neuron, instead of a full perforation as you would expect, it only
   > results in a partial perforation.


Not sure what a partial perforation is. Are you in fact getting
a proper seal? I suppose the ampB could go bad, or it could
leak out of the pipette and permeabilize the cells as well.
Are you back filling a small volume of the ampB into a large
volume of ampB-free buffer in the pipette?


Cheers,


Dominic


From owner-biophys@hgmp.mrc.ac.uk  Sat Sep  8 16:09:49 2001
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On 4 Sep 2001, Matthew Ireland wrote:

   > justus.verhagen@psy.ox.ac.uk (Justus Verhagen) wrote in message news:<3b949d46.521116226@news.ox.ac.uk>...
   >
   > Good question. But the amphotericin is relatively new and I checked
   > with our ordering company about the shelf life and they said that it
   > normally lasts for 3 years.

And I can confirm it normally works many, many years
after purchase.



Cheers,


Dominic


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Dominic-Luc Webb molmed <domweb@mbox.ki.se> wrote in message news:<Pine.GSO.4.33.0109081702570.14848-100000@mbox.ki.se>...
> On 3 Sep 2001, Matthew Ireland wrote:
> 
>    > Dear Colleagues
>    >
>    > Currently I am using perforated patch on neurons within the CNS.
>    > Recently, I have been experinceing a problem when I try to perforate a
>    > neuron, instead of a full perforation as you would expect, it only
>    > results in a partial perforation.
> 
> 
> Not sure what a partial perforation is. Are you in fact getting
> a proper seal? I suppose the ampB could go bad, or it could
> leak out of the pipette and permeabilize the cells as well.
> Are you back filling a small volume of the ampB into a large
> volume of ampB-free buffer in the pipette?
> 
> 
> Cheers,

I am backfilling the electrode with an AmpB free solution and I'm
forming normal seals.

What I'm seeing, is instead of a normal perforation (ie. within 5
mins, around 15pF & 10mOhms), I see a small change in the whole cell
capacitance (4pF) that remains that way for the duration of the
experiment.

I don&#8217;t think the AmpB is leaking out of the electrode. 

With thanks
Matthew


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</FONT></P></LI></FONT></TD></TR></TBODY></TABLE></TD></TR></TABLE><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR>

<P></P><FONT size=3>Certain statements contained in this news release may be 

forward-looking statements within the meaning of&nbsp; The Private Securities 

Litigation Reform Act of 1995. These statements may be identified by such terms 

as "expect", "believe", "may", "will", and "intend" or similar terms. We are NOT 

a registered investment advisor or a broker dealer. This is NOT an offer to buy 

or sell securities. No recommendation that the securities of the companies 

profiled should be purchased, sold or held by individuals or entities that learn 

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information provided is for reading purposes only. If anyone decides to act as 

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attorney or a registered financial broker, if any party decides to participate 

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the investment. The profiled companies make no warranties or guarantees as to 

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additional independent research on the profiled companies in order to allow the 

investor to form his or her own opinion and decision regarding investing in the 

profiled companies. Factual statements made by the profiled companies are made 

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there has been no change in the affairs of the company profiled since the date 

of review. Investing in micro-cap securities is highly speculative and carries 

an extremely high degree of risk and uncertainties. For further details 

concerning these risks and uncertainties, see the SEC filings of CBYI including 

the company's most recent annual and quarterly reports. It is possible that an 

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Subsequently we may from time to time buy or sell shares of CBYI in the open 

market. All information provided on the profiled companies may include 

information provided by outside sources, such as research reports, public 

filings, web sites or computer databases. Copyright © 2001 </FONT></BODY></HTML>

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From owner-biophys@hgmp.mrc.ac.uk  Tue Sep 18 17:27:06 2001
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Im looking for protocols for growing cells in hydrogels.



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From owner-biophys@hgmp.mrc.ac.uk  Sun Sep 23 20:55:36 2001
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From: daniel@seaoflife.co.il ("Uri Ben Hur")
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Subject: "..And the Dead Sea became the Sea of Life.."
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Subject: 2.9% Visa !               skjxad
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            CHECK BY FAX SERVICES!

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**********************************************************

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**********************************************************

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-=-=-=-=--=-=-=-=-=-=-=Remove Instructions=-=-=-=-=-=-=-=-=-=-=-=

**************************************************************
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---


From owner-biophys@hgmp.mrc.ac.uk  Sun Sep 30 19:55:39 2001
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------=_NextPart_000_3D94_000053D8.00001E00
Content-Type: text/html;

<HTML>
<BODY>

<FONT face="MS Sans Serif">
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your domain more traffic!<BR>
<BR>
WE submit your domain to 69 (sixty-nine)<BR>
search engines!<BR>
<BR>
</FONT>
<FONT color="#FF0000"><B> It's simple</B></FONT>
<FONT color="#000000">  - WE get you indexed on more<BR>
search engines and <BR>
Drive more traffic to your domain! <BR>
<BR>
We compiled a list of the major<BR>
international and domestic search engines!<BR>
<BR>
This is your opportunity to increase<BR>
your domain's traffic, <BR>
Quickly!<BR>
<BR>
WE update </FONT>
<FONT color="#000000"><B> (submit) </B></FONT>
<FONT color="#000000"> your domain weekly or<BR>
as needed plus, WE provide you with a monthly<BR>
report </FONT>
<FONT color="#FF00FF"> (by request only!) </FONT>
<FONT color="#000000"> <BR>
<BR>
</FONT>
<FONT color="#0000FF"><B> Here's the complete list:<BR>
</B></FONT>
<FONT color="#000000"><B> AltaVista<BR>
HotBot<BR>
InfoSeek/Go Network (Web Results) <BR>
InfoSeek/Go Network (Proven Picks)<BR>
AOL Search<BR>
Direct Hit <BR>
Excite<BR>
Fast/All The Web<BR>
Google<BR>
Goto.com<BR>
IWon<BR>
Lycos<BR>
MSN<BR>
NBCi (Formerly Snap)<BR>
Netscape<BR>
Open Directory <BR>
WebCrawler   <BR>
Yahoo<BR>
Plus<BR>
Acoon (Germany)<BR>
Aeiwi (USA)<BR>
AltaVista (France)<BR>
AltaVista (Germany)<BR>
AltaVista (Itlay)<BR>
AltaVista (Switzerland)<BR>
Blitz (Germany)<BR>
Excite (Australia)<BR>
Excite (France)<BR>
Excite (Italy)<BR>
Excite (UK)<BR>
FindOnce (UK)<BR>
Free (France)<BR>
FrequentFinders (USA)<BR>
GoEureka (Australia)<BR>
Indibil (Spain)<BR>
InfoMak (USA)<BR>
Infoseek (Germany)<BR>
InfoTiger (USA)<BR>
Lokace (France)<BR>
Lycos (Chile)<BR>
Lycos (Columbia)<BR>
Lycos (France)<BR>
Lycos (German)<BR>
Lycos (Italian)<BR>
Lycos (Japan)<BR>
Lycos (Peru)<BR>
Lycos (Spain)<BR>
Lycos (Sweden)<BR>
Lycos (UK)<BR>
MaxiSearch (Germany)<BR>
Origo (Norway)<BR>
QuestFinder (USA)<BR>
SearchEngine (UK)<BR>
Sharelook (Italy)<BR>
ShinySeek (Italy)<BR>
Speedfind (Germany)<BR>
SplatSearch (USA)<BR>
Spray (France)<BR>
SunSteam (Norway)<BR>
SuperSnooper (USA)<BR>
Superva (Italian)<BR>
Sympatico (Canadian)<BR>
UK Max (UK)<BR>
Unearth (New Zealand)<BR>
Voila (France)<BR>
Voila (Spain)<BR>
Walhello (USA)<BR>
WebSearch (Australia)<BR>
WebWombat (Australia)<BR>
Yagua (Paraguay)<BR>
</B></FONT>
<FONT color="#000000"> <BR>
</FONT>
<FONT color="#FF0000"><B> It's easy!!<BR>
</B></FONT>
<FONT color="#000000"> <BR>
Just fax or mail the following to:<BR>
</FONT>
<FONT color="#000000"><B> FAX:</B></FONT>
<FONT color="#000000">  </FONT>
<FONT face="Times New Roman">
<FONT size=3><B> 1-(413) 638-8510</B> <BR>
<FONT face="MS Sans Serif">
<FONT size=2>
<FONT color="#000000"> <BR>
</FONT>
<FONT color="#000080"><B> US MAIL:<BR>
</B></FONT>
<FONT color="#000000"><B> CyberCO<BR>
8190 W Deer Valley Road Suite 104-265<BR>
Peoria, AZ 85382<BR>
</B></FONT>
<FONT color="#000000"> <BR>
</FONT>
<FONT color="#000000"><B> WE WILL CONTACT YOU IN 2 BUSINESS DAYS<BR>
AND WE DO NO START THE WORK UNTIL <BR>
WE SPEAK WITH YOU!!</B></FONT>
<FONT color="#000000"> <BR>
<BR>
<BR>
-- Order Form --<BR>
<BR>
PAYMENT TYPES:<BR>
<BR>
CHECK  -  CREDIT CARD  -  MONEY ORDER<BR>
<BR>
All orders must be completely filled<BR>
out!<BR>
<BR>
Company Name:<BR>
<BR>
Address:<BR>
<BR>
State:                       City:      <BR>
Zip:<BR>
<BR>
Contact Name:<BR>
<BR>
Contact Telephonel:<BR>
<BR>
Contact Fax:<BR>
<BR>
Contact E-mail Address:<BR>
<BR>
Web site Address:<BR>
<BR>
<BR>
A few of your top Keywords:<BR>
<BR>
Questions:<BR>
<BR>
Credit Card #.<BR>
<BR>
Name On Card.<BR>
<BR>
Sign Here:__________________________________<BR>
<BR>
Date:_____________<BR>
<BR>
 --***  NOTE  ***--<BR>
 348.00 US Dollars WILL COVER 12 MONTHS<BR>
OF SERVICE.<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
<BR>
--------------------------------------<BR>
--------------------------------------<BR>
To be removed from future mailings!!!!<BR>
All REMOVE requests AUTOMATICALLY<BR>
honored upon receipt.<BR>
href="mailto:sendhere11@excite.com?subject=NOBIZ"mail<BR>
to:<BR>
sendhere11@excite.com?subject=NOBIZ<BR>
PLEASE understand that any effort to disrupt, close or block <BR>
this REMOVE account can only result in difficulties for others <BR>
wanting to be removed from our mailing list as it will be impossible <BR>
to take anyone off the list if the remove instruction is not received.<BR>
<BR>
<BR>
----------------------------------------<BR>
----------------------------------<BR>
<BR>
<BR>
<BR>
<BR>
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