From owner-biotechniques@net.bio.net Fri Jul 21 23:00:00 1995 Path: biosci!biosci!not-for-mail From: kristoff@net.bio.net (David Kristofferson) Newsgroups: bionet.journals.letters.biotechniques Subject: test of bionet.journals.letters.biotechniques Date: 22 Jul 1995 06:06:45 -0700 Organization: BIOSCI International Newsgroups for Biology Lines: 11 Approved: biotechniques-moderator@net.bio.net Distribution: world Message-ID: <3uqt55$h61@net.bio.net> NNTP-Posting-Host: net.bio.net test of bionet.journals.letters.biotechniques. This moderated newsgroup is not yet ready for operation. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-biotechniques@net.bio.net Tue Jul 25 23:00:00 1995 Path: biosci!biosci!not-for-mail From: Dave Kristofferson Newsgroups: bionet.journals.letters.biotechniques Subject: test of bionet.journals.letters.biotechniques Date: 26 Jul 1995 13:29:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <3v68jp$ho4@net.bio.net> NNTP-Posting-Host: net.bio.net This test message will be posted to the newsgroup by the moderator. From owner-biotechniques@net.bio.net Tue Jul 25 23:00:00 1995 Path: biosci!biosci!not-for-mail From: biohelp@net.bio.net (BIOSCI Administrator) Newsgroups: bionet.journals.letters.biotechniques Subject: BIOTECHNIQUES/bionet.journals.letters.biotechniques is ready! Date: 26 Jul 1995 13:55:09 -0700 Organization: BIOSCI International Newsgroups for Biology Lines: 231 Approved: biotechniques-moderator@net.bio.net Distribution: world Message-ID: <3v6a3d$jd2@net.bio.net> NNTP-Posting-Host: net.bio.net The BIOTECHNIQUES/bionet.journals.letters.biotechniques newsgroup is ready for operation. This newsgroup is moderated, i.e., all postings are forwarded to a moderator/editor and are reviewed prior to distribution. The newsgroup charter is appended below followed by detailed usage instructions and information on how to search the newsgroup archives. *Please save* this message for future reference if you intend to participate in this newsgroup. ---------------------------------------------------------------------- Moderator: Mary McCarthy Associate Editor/BioTechniques mmccarthy@biotechnet.com Charter: A new newsgroup, "BIOTECHNIQUES", is proposed to provide a forum for the discussion of methods, and appropriate related issues, that have appeared on the pages of BioTechniques - an international, peer-reviewed journal which describes laboratory techniques in the life sciences. The newsgroup will also function as a rapid-communication, electronic counterpart of the "BioFeedback" section of BioTechniques. Participation in the newsgroup should be of value to individuals, including the novice as well as the experienced scientist, who are interested in the development and application of laboratory methods. The newsgroup will be moderated by Mary McCarthy, Associate Editor/BioTechniques. ---------------------------------------------------------------------- SUBSCRIPTION/USAGE INFORMATION PLEASE NOTE that many USENET sites do not allow automatic creation of new USENET groups!!! If you do not see bionet.journals.letters.biotechniques in your newsreader within another day or two, ask your news system administrator to act on our "newgroup" message to enable the group at your site. We have already done several tests and are certain that the group is currently propagating around the network. If he/she can not find the newsgroup message, have them retrieve the bionet checkgroups message from the anonymous FTP area on net.bio.net in pub/BIOSCI/doc/bionet-checkgroups-msg. 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ALWAYS be certain that you examine the address on your messages before you send them!!! Since this is a moderated group, you may be able to cancel a message sent by mistake if you contact the moderator promptly before he/she posts the message. On unmoderated newsgroups, there would be no way to stop a mistaken message. Some non-Internet compliant mail systems may attempt to send replies to our error-trapping address called BIOSCI-REQUEST. If yours does this, please be sure to readdress your message to btechniq@net.bio.net or btechniq@daresbury.ac.uk if you want to send it to the newsgroup. How to look at archives of the list: ------------------------------------ Archives for BIOTECHNIQUES/bionet.journals.letters.biotechniques are kept in the anonymous FTP account at net.bio.net [204.31.212.2]. Look in the directory pub/BIOSCI/BIOTECHNIQUES for posting archives. Each file is assigned a date such as 9501 for January 1995. Please note that ours is a UNIX system and all file and directory names are case-sensitive, i.e., upper case file names are different from lower case names. You can also access these same files via Gopher if you start a gopher session using net.bio.net as your gopher server. Gopher also allows you to view the individual messages within each monthly archive file. The files are in the BIOTECHNIQUES directory. Postings to bionet.journals.letters.biotechniques are also WAIS indexed and can be searched via either gopher or WAIS at our site. In gopher the option at net.bio.net is "Search Bionet USENET Articles" and in WAIS one should use the WAIS source biosci.src. This is a WAIS index of all BIOSCI/bionet messages including this newsgroup. Please see the BIOSCI FAQ for details. The FAQ can be requested from biosci-help@net.bio.net. Finally, you can also access the archives via WWW/Mosaic. The URL is http://www.bio.net/ and you can run the WAIS archive searches in your WWW browser software window after connecting to our site. Once again, if you have any administrative questions that require personal assistance, please address them to biosci-help@net.bio.net in the U.S. or biosci@daresbury.ac.uk in the UK. Best wishes for a successful newsgroup! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-biotechniques@net.bio.net Mon Jul 31 23:00:00 1995 Path: biosci!biosci!not-for-mail From: pnh@fcsparc6.ncifcrf.gov (Paul N Hengen) Newsgroups: bionet.journals.letters.biotechniques Subject: Page Numbers for Articles in BIOTECHNIQUES Date: 1 Aug 1995 12:58:27 -0700 Organization: Frederick Cancer Research and Development Center Lines: 26 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: bionet Message-ID: <3vm113$gq3@net.bio.net> NNTP-Posting-Host: net.bio.net Summary: Why not put the page numbers on ALL pages? Mary McCarthy Associate Editor/BioTechniques mmccarthy@biotechnet.com Dear Mary: This posting is not about a specific technique, but about something that I've been meaning to bring to the attention of the editors of your journal for some time. Many of the printed articles are interrupted by advertisements and since not all the pages of BioTechniques have the page numbers on them, photocopied articles are difficult to reference without looking them up in the original volume. This can be very frustrating when a number of articles are to be referenced at one time, forcing one to go to the library and look all of them up again to be sure of the correct page numbers. It would be a vast improvement if the numbers were printed on ALL pages, or the page numbers for the reference printed on the first page of the article itself as many other journals do. ******************************************************************************* * Paul N. Hengen, Ph.D. /--------------------------/* * National Cancer Institute |Internet: pnh@ncifcrf.gov |* * Laboratory of Mathematical Biology | Phone: (301) 846-5581 |* * Frederick Cancer Research and Development Center| FAX: (301) 846-5598 |* * Frederick, Maryland 21702-1201 USA /--------------------------/* * - - - - - Methods FAQ list -> http://www-lmmb.ncifcrf.gov/~pnh/ - - - - - - * * - - - Anonymous FTP from ftp.ncifcrf.gov as file pub/methods/FAQlist - - - * ******************************************************************************* From owner-biotechniques@net.bio.net Mon Jul 31 23:00:00 1995 Path: biosci!biosci!not-for-mail From: hoekstra@unixg.ubc.ca (Sucking Gippy) Newsgroups: bionet.journals.letters.biotechniques Subject: Preferences in Immunohistochemistry Date: 1 Aug 1995 14:00:10 -0700 Organization: University of British Columbia, Vancouver, B.C., Canada Lines: 12 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <3vm4kq$kak@net.bio.net> NNTP-Posting-Host: net.bio.net I am soon to embark on immunohistochemical techniques to see specific proteins in tissue samples. Having never used these techniques before, I was wondering if people would share their preferences with regards to using cryo-sectioned tissues, cryo-sectioned tissues--and then fixed, and wax embedding tissues--usually using paraformaldehyde prior to embedding. Which do people prefer? And from personal experience, which have led to more successful preparation in the end? I will be analyzing the aorta, heart, liver and spleen