From owner-biotechniques@net.bio.net Mon Apr 01 23:00:00 1996 Path: biosci!biosci!not-for-mail From: hardings@delta.newi.ac.uk Newsgroups: bionet.journals.letters.biotechniques Subject: plant metallothionines Date: 2 Apr 1996 09:08:19 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <4joleg$f1e@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net can anyone please tell me of any purification protocols for plant metallothionnes? stephen harding From owner-biotechniques@net.bio.net Wed Apr 03 23:00:00 1996 Path: biosci!biosci!not-for-mail From: hardings@delta.newi.ac.uk Newsgroups: bionet.journals.letters.biotechniques Subject: plant metallothionines Date: 4 Apr 1996 05:36:45 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <4k084i$pij@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net can anybody tell me of any references or protocols for the production of plant metallothionines?also references or protocols for the purification of the produced proteins? thanks steve harding From owner-biotechniques@net.bio.net Mon Apr 08 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Donald Lo Newsgroups: bionet.journals.letters.biotechniques Subject: (no subject) Date: 9 Apr 1996 06:36:31 -0700 Organization: Department of Neurobiology, Duke University Lines: 4 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <199604081853.OAA18327@neuro.duke.edu> NNTP-Posting-Host: net.bio.net Does anyone out there know if anyone sells biotinylated luciferase? (or avidin-luciferase, for that matter?) From owner-biotechniques@net.bio.net Fri Apr 12 23:00:00 1996 Path: biosci!biosci!not-for-mail From: MMCCARTHY@biotechnet.com Newsgroups: bionet.journals.letters.biotechniques Subject: BioTechniques 20(5), May 1996 Date: 12 Apr 1996 22:53:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 129 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <01I3F22ZM4XU96WTK3@delphi.com> NNTP-Posting-Host: net.bio.net BioTechniques 20(5), May 1996 Table of Contents BENCHMARKS Fluorescence in situ hybridization in whole-mount Drosophila embryos -Hughes, S.C., Saulier-Le Drean, B., Livne-Bar, I. and =20 Krause, H.M. Direct analysis of in vitro transcription products from supercoiled templates containing ribozymes -Batt, D.B. Heterogeneity of DNA binding to membranes used in quantitative dot blots -Anchordoguy, T.J., Crawford, D.L., Hardewig, I. and =20 Hand, S.C. Microsatellite libraries enriched for several microsatellite sequences in plants -Edwards, K.J., Barker, J.H.A., Daly, A., Jones, C. and Karp, A. Broad-host-range cloning and cassette vectors based on R388 trimethoprim resistance gene -DeShazer, D. and Woods, D.E. PCR-based quantitation of transfected luciferase DNA: evaluation using an Entamoeba histolytica reporter construct.=20 -Gilchrist, C.A., Streets, H.L., Ackers, J.P. and Hall, R. LA-PCR-based quick method for the identification of genes responsible for the complementation of Saccharomyces cerevisiae mutations -Ohya, Y. Identification of introns by Reverse-Transcription PCR -Alexander, H. and Alexander, S. Use of elevated reverse transcription reaction temperatures in RT-PCR -Freeman, W.M., Vrana, S.L. and Vrana, K.E. Isolation of DNA from stool and bodily fluids for PCR amplification -Nollau, P., Moser, C. and Wagener, C. Simultaneous extraction of DNA and RNA from nuclease-rich pathogenic protozoan Trichomonas vaginalis -Chou, C.-F. and Tai, J.-H. Reducing "double sequences" in automated DNA sequencing with T7 DNA polymerase and internal labeling -Wiemann, S., Schilke, A., Rechmann, S., Zimmermann, J., Voss H. and Ansorge, W. Lysis buffer composition dramatically affects extraction of phosphotyrosine-containing proteins -Ignatoski, K.M.W. and Verderame, J.F. Improved technique for the preparation of water-in-oil emulsions containing protein antigens -Vulliet, R. Robotic automation of Xenopus oocyte bath perfusion -Mihic, S.J. and Harris, R.A. Single step purification/solubilization of recombinant proteins: application to surfactant protein B -Holzinger, A., Phillips, K.S. and Weaver, T.E. Quantitative dot-immunoblot assay to measure protein in multiple brain samples -Mink, R.B. and Johnston, J.A. Conversion of pipette-tip boxes into secure storage containers for microcentrifuge tubes -Hendry, I.R. and Hendry III, W.J. Screening for recombinant plasmids in yeast colonies of the two-hybrid system using PCR -Schenk, J.A., Heymann, S., Peters, L.E. and Micheel, B. THE INTERNET ON-RAMP (Cyberspace for Biologists) SHORT TECHNICAL REPORTS Transient transfection of primary cultured hepatocytes using CaPO4/DNA precipitation -Gaunitz, F., Papke, M. and Gebhardt, R. In vivo chromatin structure of the murine interleukin-5 gene region: a new intact cell system.=20 -Ymer, S. and Jans, D.A. Single-step immunocapture RT-PCR in the detection of raspberry bushy dwarf virus -Kokko, H.I., Kivineva, M. and K=84renlampi, S.O. Single-tube protocol for the extraction of DNA or RNA from paraffin-embedded tissues using a starch-based adhesive.=20 -Turbett, G.R., Barnett, T.C., Dillon, E.K. and =20 Sellner, L.N. Improved method for the production of M13 phage and single-stranded DNA for DNA sequencing -Reddy, P. and McKenney, K. Sequencing homopolymer tracts and repetitive elements.=20 -Robbins, C.M., Hsu, E. and Gillevet, P.M. Control system for cDNA enrichment reactions -Gastel, J.A. and Sutter, T.R. LAB SAFETY TIPS Prevention of radioactive contamination of cell culture incubators during metabolic labeling experiments -Fornerod, M. Economical apparatus for safe, accurate recovery of biohazardous or radioactive gradient fractions -Canon, J. and Krogstad, P. BIOFEEDBACK RESEARCH REPORTS Multiplex sets for the amplification of polymorphic short tandem repeat loci - silver stain and fluorescent detection -Lins, A.M., Sprecher, C.J., Puers, C. and Schumm, J.W. Rapid, High-level transient expression of papillomavirus-like particles in insect cells -Benincasa, D., Silberklang, M., Mark III, G.E., and =20 Ludmerer, S.W. CD-tagging: a new approach to gene and protein discovery and analysis -Jarvik, J.W., Adler, S.A., Telmer, C.A., Subramaniam, V. and Lopez, A.J. Glycerol enhancement of ligand-polylysine/DNA transfection -Zauner, W., Kichler, A., Schmidt, W., Sinski, A. and =20 Wagner, E. PRODUCT APPLICATION FOCUS Accelerating the pace of luciferase reporter gene assays -Roelant, C.H., Burns, D.A. and Scheirer, W. Better gel resolution and longer cDNAs increase the precision of differential display -Averboukh, L., Douglas, S.A., Zhao, S., Lowe, K., =20 Maher, J. and Pardee, A.B. NEW PRODUCTS INDEX TO ADVERTISERS From owner-biotechniques@net.bio.net Tue Apr 23 23:00:00 1996 Path: biosci!biosci!not-for-mail From: perrinob@ohsu.edu (Brian Perrino) Newsgroups: bionet.journals.letters.biotechniques Subject: expression vector Date: 24 Apr 1996 06:36:56 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I am looking for a prokaryotic protein expression vector that carries a tetracycline, or gentamycin resistance gene, and in which protein expression is induced by IPTG. reply to perrinob@OHSU.edu From owner-biotechniques@net.bio.net Tue Apr 30 23:00:00 1996 Path: biosci!biosci!not-for-mail From: "Vladimir Yu. Kuryshev" Newsgroups: bionet.journals.letters.biotechniques Subject: I'm looking for Microplate Reader's info Date: 1 May 1996 05:47:00 -0700 Organization: SPb State University Lines: 17 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <4m0u7b$odd@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net Hi, Netters, Our Lab are planning to buy Microplate Reader for ELISA-tests. I'd like to collect some information about this equipment and appropriate software. I will be very obliged to you if somebody shares your information (names, addresses, fax, e-mail or URL is preferable) of firms which produce or sale the Microplate Readers. Will be glad to get any your advices for making a good choice. Thank you in advance. Volodia P.S. Firms in Russia are preferable, but not exclusive