From owner-biotechniques@net.bio.net Tue May 28 23:00:00 1996 Path: biosci!biosci!not-for-mail From: MMCCARTHY@biotechnet.com Newsgroups: bionet.journals.letters.biotechniques Subject: BioTechniques 20(6), June 1996 Date: 29 May 1996 05:49:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 142 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <01I59SSG1ZSY984WRL@delphi.com> NNTP-Posting-Host: net.bio.net BioTechniques 20(6), June 1996 Table of Contents BENCHMARKS Joining of DNA fragments by repeated cycles of denaturation, annealing and extension -Kim J., M. Puder and R.J. Soberman Differentiating allele combinations of a transgene using multiple primer PCR. -Pekhletski R. and D.R. Hampson An in vitro assay of =E1-galactosidase from yeast -Schneider S., M. Buchert and C.M. Hovens Improved in situ hybridization: color intensity enhancement procedure for the alkaline phosphatase/Fast Red system. -Chiu K.-p., T. Sullivan and S. =20 Bursztajn Use of UV methods for measurement of protein and nucleic acid concentrations -Manchester K.L. Reusing the same blood-stained punch for sequential DNA amplifications and typing -Del Rio S.A., M.A. Marino and P. =20 Belgrader DNA extraction technique for PCR in Rain Forest Plant Species -Scott K. and J. Playford Direct PCR-screening of Pichia pastoris clones -Linder S., M. Schliwa and E. =20 Kube-Granderath Trichloroacetic acid precipitation by ultracentrifugation to concentrate dilute protein in viscous solution -Hwang B.J. and G. Chu A modified unique site elimination mutagenesis in constructing a chloramphenicol resistance-encoding pGEM vector -Young C., K. Matsubara and C. Nitz Bi-directional Dideoxy Fingerprinting (Bi- ddF): Rapid and Efficient Screening for Mutations in the Big BlueTM Transgenic Mouse Mutation Detection System -Haavik, J., H. Nishino, Q. Liu and S.S. Sommer THE INTERNET ON-RAMP SHORT TECHNICAL REPORTS Modulaton of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. -Brownstein M.J., J.D. Carpten and J.R. Smith Optimization of DNase I removal of contaminating DNA from RNA for use in quantitative RNA-PCR -Huang Z., M.J. Fasco and L.S. Kaminsky Method for 96-well M13 DNA template preparations for large scale sequencing -Andersson B., J. Lu, K.E. Edwards, D.M. Muzny and R.A.Gibbs BIOFEEDBACK RESEARCH REPORTS Differential display protocol with selected primers that preferentially isolates mRNAs of moderate-to-low abundance in a microscopic system -Ikonomov O.C. and M.H. Jacob Generation of sequence signatures from DNA amplification fingerprints with mini-hairpin and microsatellite primers -Caetano-Anoll=82s G. and P.M. Gresshoff DNA sequencing by capillary electrophoresis using short oligonucleotide primer libraries -Ruiz-Martinez M.C., E. Carrilho, J. =20 Berka, J. Kieleczawa, A.W. Miller, F. =20 Foret, S. Carson and B.L. Karger Phage display shot-gun cloning of ligand binding domains of prokaryotic receptors approaches 100% correct clones -Jacobsson K. and L. Frykberg BIOCOMPUTING System for accurate one-dimensional gel analysis including high resolution quantitative footprinting -Smith J. and M. Singh Spreadsheet macro for setting up PCR assay tubes -Stowe R.P. and D.L. Pierson PRODUCT APPLICATION FOCUS Software to determine optimal oligonucleotide sequences based on hybridization simulation data -Hyndman D., A. Cooper, S. Pruzinsky, D. Coad and M.Mitsuhashi Selection of murine lymphoid and hematopoietic cells using polystyrene tissue culture devices containing covalently immobilized antibody -Berson A.E., K.M. Knobel, D. Rood, K. =20 Chen, D. Lamons, M.A.McNally, T.B. =20 Okarma and J.S. Lebkowski 1996 AUTHORS INDEX (Volume 20) NEW PRODUCTS INDEX TO ADVERTISERS From owner-biotechniques@net.bio.net Tue May 28 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Ronald Alexander Newsgroups: bionet.journals.letters.biotechniques Subject: DNA Extraction from Fecal Samples Date: 29 May 1996 13:04:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <31A61763.4FD3@sirius.com> NNTP-Posting-Host: net.bio.net I was hoping someone could lead me to some protocols for extracting DNA from gram+ bacteria that are present in feces. Please send replies to: jabrwock@sirius.com Thank you, Ron From owner-biotechniques@net.bio.net Tue May 28 23:00:00 1996 Path: biosci!biosci!not-for-mail From: MMCCARTHY@biotechnet.com Newsgroups: bionet.journals.letters.biotechniques Subject: BioTechniques Database on the Web Date: 29 May 1996 06:05:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <01I59T88KD2Q984WRL@delphi.com> NNTP-Posting-Host: net.bio.net The BioTechniques database is now available on the BioTechniques home page at http://www.BioTechniques.com Restricted and inclusive searches can be conducted by title, author, keyword, abstract and "all text". Check it out! The site is still under construction and suggestions for improvements are welcome. Mary McCarthy Associate Editor From owner-biotechniques@net.bio.net Thu May 30 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Chromosome Terror Newsgroups: bionet.journals.letters.biotechniques Subject: Genomic labelling in-situ of plant DNA Date: 31 May 1996 05:40:46 -0700 Organization: University of Reading, U.K. Lines: 21 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I am working with wheat. I want to intercross two lines, but I want to keep track, in the very early embryo and endosperm development, of the parental chromosomes. I thought of introducing tritiated thimidine into the spikes, previous to meiosis. Any other way of labelling, not involving tritium? Any thoughts as to what would be the best way to introduce the labelled compound into the plant? I thought of putting a fine thread through the stem, wetted in the labelling solution, and let the transpiration flow carry the label to the pollen mother cells, and to the eggs. Any better ways? Thank you for any help Nacho de las Heras Botany Dept. University of Reading Reading UK