From owner-biotechniques@net.bio.net Thu Oct 03 23:00:00 1996 Path: biosci!biosci!not-for-mail From: anton beletskii Newsgroups: bionet.journals.letters.biotechniques Subject: Site-Directed Mutagenesis...,BioTechniques 21 p.208 (1996) Date: 4 Oct 1996 06:33:24 -0700 Organization: Wayne State University, Detroit, MI 48201 Lines: 14 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <32542641.13A@chem.wayne.edu> NNTP-Posting-Host: net.bio.net I was pleased to find modification of the unique site elimination protocol in August issue of the BioTechniques (B.Ogretmen, C.Carpo and A.Safa, Site-Directed Mutagenesis by Unique Site Elimination Using Filamentous Phage-Derived ssDNA Templates for Plasmids That Are Resistant to Denaturation/Uf...who came up with the name like this!?/, Biotechniques, 1996, 21(2), 209-213). But there is a mistake in the composition of synthesis buffer which would probably make the protocol not working. In addition to four dNTPs, synthesis buffer should contain 1mM rATP. In my hands, addition of the T4 Gene 32 Protein (Pharmacia) at final conc. 5ug/ul greatly improves extension/ligation step, for regular USE mutagenesis. It is interesting if it will improve this protocol. Also, after addition of T4 DNA polymerase, keeping extension/ligation reaction on ice for 5 min, then at r.t. for 5 min and as usual 2 h at 37 C helps somewhat. I wish I see more mutagenesis papers in Biotechniques. From owner-biotechniques@net.bio.net Sat Oct 05 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Your_name Last_name Newsgroups: bionet.journals.letters.biotechniques Subject: non-radioactive DD Date: 6 Oct 1996 10:46:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Sirs. I would need quick information about performing non-radioactive differential display and silver-staining of the gel. I know that somebody published something concerning this protocol, but I couldn't find the original article. I thank evrybody who can help me. Please mail to Dr.Claudio Luparello, Dipartimento di Biologia Cellulare e dello Sviluppo, Viale delle Scienze, Palermo (Italy). Fax +39 91 420897, E-mail: cllup@mbox.vol.it From owner-biotechniques@net.bio.net Tue Oct 08 23:00:00 1996 Path: biosci!biosci!not-for-mail From: rtewari27@aol.com (RTewari27) Newsgroups: bionet.journals.letters.biotechniques Subject: transgenics Date: 9 Oct 1996 05:31:43 -0700 Organization: America Online, Inc. (1-800-827-6364) Lines: 17 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <53f604$ekh@newsbf02.news.aol.com> NNTP-Posting-Host: net.bio.net Hi: I am a graduate student at Rutgers University, New Jersey. I am doing a classroom research project, where I am trying to find out the different research groups all over North America who are using the Transgenic technology for their research purposes (either for academic or commercial purposes). Specifically, I am more interested in getting information on groups which are working with the Microinjection technique. I know that this list can be considerably extensive, but I am trying to get as much information as I possibly can. I would really appreciate some help with this project. I would even like it if I could get some pointers on how to go about getting this information. Thank you very much Rashmi Tewari email add: rtewari27@aol.com From owner-biotechniques@net.bio.net Wed Oct 09 23:00:00 1996 Path: biosci!biosci!not-for-mail From: lorraine@nature.berkeley.edu (Lorraine Sohlberg) Newsgroups: bionet.journals.letters.biotechniques Subject: Chromosome in situ hybridization to localize single copy genes Date: 10 Oct 1996 05:51:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <53irfk$8a9@net.bio.net> NNTP-Posting-Host: net.bio.net Hello, I am developing techniques at the NSF Center for Plant and Microbial Developmental Biology at Univ of Calif., Berkeley. I have used in situ hybridization to identify the chromosomal position of a tandem repeat sequence and am now I am interested in using in situ hybridization to detect single copy genes on maize, corn, meiotic pachytene chromosomes. This hasn't been done much in plants and I was wondering if I could get feedback from the world of bionet about successful techniques. I have a few questions regarding the probes used. I understand that the cloned sequence used to create the probe must be rather large in order to get a decent signal. In addition it can not contain any repeated sequence in an intron or spacer region that my light up elsewhere in the genome. I was also wondering how the probes are synthesized. I have been using DIG labelled RNA probes and they work well to locate the tandem repeat. Has anyone used PRINS or other PCR techniques? Thanks for your help, in advance, Lorraine From owner-biotechniques@net.bio.net Wed Oct 09 23:00:00 1996 Path: biosci!biosci!not-for-mail From: "Safa@musc.edu (Eddie Choi)" Newsgroups: bionet.journals.letters.biotechniques Subject: Re: Site-Directed Mutagenesis Date: 10 Oct 1996 06:41:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net It should be noted that the synthesis buffer used in our protocol that we described recently (Ogretmen, B., Carpo, C. and Safa, A. R., Site-directed mutagenesiss by unique site elimination using filamentous phage-derived ssDNA templates for plasmids that are resistant to denaturation. BioTechniques 21:209-213, 1996) was obtained from ClonTech (Palo Alto, CA) with the Transformer site-directed mutagenesis kit, as mentioned in the manuscript. The content of the buffer is as described by Deng and Nickoloff, Analytical Biochemistry 200:81-88, 1992, containing Tris-Hcl (100 mM, pH 7.5), four dNTP's (5mM/each), ATP (10mM) and DTT (20 mM). Sincerely, Ahmad R. Safa, PhD. From owner-biotechniques@net.bio.net Mon Oct 14 23:00:00 1996 Path: biosci!biosci!not-for-mail From: MMCCARTHY@biotechnet.com Newsgroups: bionet.journals.letters.biotechniques Subject: BioTechniques 21(5), November 1996 Date: 15 Oct 1996 13:00:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 182 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <01IAOEIS9DLE8ZGDQV@delphi.com> NNTP-Posting-Host: net.bio.net BioTechniques 21(5), November 1996 Table of Contents BENCHMARKS Long PCR: selective suppression by restriction endonuclease digestion -Her C. and R. Weinshilboum Simplified protocol of solid-phase cDNA libraries for multiple PCR amplification -Fellmann F., J.-L. Pretet and D. - Fellmann Removal of RT-PCR inhibitors from RNA extracts of tissues -Mathy N.L., R.P. Lee and J. Walker Oven cooking bags allow an inexpensive alternative in oil-free competitive RT-PCR -Houze T.A. and B. Gustavsson Evaluation of anchorage-independent proliferation in tumorigenic cells using the redox dye alamarBlueTM -Gray G.D. and E. Wickstrom Removing monolayer cells from culture dishes by incubation with EDTA in studies of cell surface receptors -Hu X. and K.S. Zuckermann Spin columns can be used to study G protein-coupled receptor binding -Popp M.P., F.L. Tomson and J.H. McDowell Importance of liposome complexing volume in transfection optimization -Staggs D.R., D.W. Burton and L.J. Deftos Simultaneous preparation of RNA and nuclei for Northern blot and flow cytometric analysis -Tesfaigzi J. and R. Jaramillo Use of a DNA sequencing gel apparatus for analysis of polypeptides -Whelan K.F. and D.E. Taylor Antibody screening for secreted proteins expressed in Pichia pastoris -Wung J.L. and N.R.J. Gascoigne Detection of early and late stage of apoptosis with field inversion gel electrophoresis -Luo Y. and D. Kessel Removal of Coomassie blue precipitates from polyacrylamide gels -Lewis S.A. Modified crush-and-soak method for recovering oligodeoxynucleotides from polyacrylamide gel -Chen Z. and D.E. Ruffner Use of engineered thrombin cleavage site for determination of translational reading frames -Zeng G. and T.J. Larson Preparation of miniprep-DNA for automated nonradioactive sequencing -Zimmermann R., C. Ullmann and W. Schaper Simplified method for ligase-free cloning of polymerase chain reaction products -Temesgen B. and K. Eschrich THE INTERNET ON-RAMP -Cyberspace for Biologists SHORT TECHNICAL REPORTS Analysis of loss of heterozygosity in microdissected tumor cells from cervical carcinoma using fluorescent dUTP labeling of PCR products -Magnusson P.K.E., E. Wilander and U. Gyllensten Improved "activator trap" method for the isolation of transcriptional activation domains from random DNA fragments -Escher D. and W. Schaffner Conversion of bacterial gene products to secretion-competent fusion proteins -Mollenkopf H.-J., I. Gentschev and W. Goebel Quantification of mRNA using competitive RT-PCR with standard curve methodology -Tsai S.J. and M.C. Wiltbank Construction of an internal control to quantitate multiple porcine cytokine mRNAs by RT-PCR -Reddy N.R.J., B.N. Wilkie and B.A. Mallard Magnetic selection of transiently transfected cells -Schneider S. and S. Rusconi Microbiological assay for the quantitative determination of glutathione -Schmidt M., M. Grey and M. Brendel RESEARCH REPORTS Solid-phase metal chelate assay for quantifying total protein: resistance to chemical interference -Lim M.J., W.F. Patton, N. Shojaee and D. Shepro Protection of DNA during preparative agarose gel electrophoresis against damage induced by ultraviolet light -Grundemann D. and E. Schomig Identification of plasminogen in Matrigel(TM) and its activation by reconstitution of this basement membrane extract -Farina A.R., A. Tiberio, A. Tacconelli, L. Cappabianca, A. Gulino and A.R. Mackay One-step one-lane chemical DNA sequencing by N-methylformamide in the presence of metal ions -Negri R., G. Costanzo, R. Saladino and E. Di Mauro 2-D protein crystals as an immobilization matrix for producing reaction zones in dipstick-style immunoassays -Breitwieser A., S. Kupcu, S. Howorka, S. Weigert, C. Langer, K. Hoffmann-Sommergruber, O. Scheiner, U.B. Sleytr and M. Sara Temperature-programmed capillary electrophoresis for detection of DNA Point Mutations -Gelfi C., L. Cremonesi, M. Ferrari and P.G. Righetti PRODUCT APPLICATION FOCUS Improved nucleic acid organic extraction through use of a unique gel barrier material -Murphy N.R. and R.J. Hellwig Transfection maximizer increases the efficiency of calcium phosphate transfections with mammalian cells -Zhang G. and S.R. Kain NEW PRODUCTS INDEX TO ADVERTISERS From owner-biotechniques@net.bio.net Mon Oct 14 23:00:00 1996 Path: biosci!biosci!not-for-mail From: MMCCARTHY@biotechnet.com Newsgroups: bionet.journals.letters.biotechniques Subject: AT Biochem Query Date: 14 Oct 1996 17:48:43 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <01IAN8XWLIK28ZGO7R@delphi.com> NNTP-Posting-Host: net.bio.net Return-path: Path: daresbury!not-for-mail Received: from mserv1.dl.ac.uk (lpddist@mserv1.dl.ac.uk) by delphi.com (PMDF V5.0-7 #10880) id <01IAN29WLL1C913TSA@delphi.com> for mmccarthy@delphi.com; Mon, 14 Oct 1996 16:56:40 -0400 (EDT) Received: by mserv1.dl.ac.uk id VAA12109 (8.6.13/5.3[ref postmaster@dl.ac.uk] for dl.ac.uk from lpddist@dl.ac.uk); Mon, 14 Oct 1996 21:54:37 +0100 Date: Mon, 14 Oct 1996 21:54:31 +0100 From: Stephen Lynas <100544.3232@CompuServe.COM> Subject: Query re: AT Biochem Sender: lpddist@dl.ac.uk To: mmccarthy@biotechnet.com, biohelp@net.bio.net Message-id: <53u9a7$bq6@mserv1.dl.ac.uk> Content-transfer-encoding: 7BIT Precedence: first-class Original-To: Biotechnique newsgroup Newsgroups: bionet.journals.letters.biotechniques Lines: 11 I am trying to find out a UK distributor for PCR Purity Plus gel solution. Can anybody give em a phone number, fax or email address? My lab is crying out for the stuff ! I can be emailed at the lab at: caroline/lynas@phnt.swest.nhs.uk TYIA Dr Caroline Lynas From owner-biotechniques@net.bio.net Wed Oct 16 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Scott Jacoves Newsgroups: bionet.journals.letters.biotechniques Subject: Trans-synaptic labeling. Date: 17 Oct 1996 06:35:57 -0700 Organization: Erindale College, University of Toronto, Canada Lines: 18 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <32654031.5CB@credit.erin.utoronto.ca> NNTP-Posting-Host: net.bio.net Hello. I'm looking for information on trans-synaptic labeling via intracellular injection. So far, the few references I've found all deal with verts, are with one exception old (of the Grafstein era), and use extracellular techniques. Does anyone have experience with trying to trace pathways or find target neurons this way, esecially in insects? The only modern reference I've found is: Peschanski, M. and Ralston, J., Light and electron microscopic evidence of transneuronal labeling with wheat germ agglutinin-horseradish peroxidase to trace somatosensory pathways to the thalamus. _Jrn. Comp. Neuro._, 236(1):29-41. 1985 Thanks, Scott Jacoves From owner-biotechniques@net.bio.net Sat Oct 19 23:00:00 1996 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.letters.biotechniques Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 20 Oct 1996 10:17:46 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <199610200900.CAA19995@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-biotechniques@net.bio.net Wed Oct 23 23:00:00 1996 Path: biosci!biosci!not-for-mail From: thehooktek@aol.com (TheHookTek) Newsgroups: bionet.journals.letters.biotechniques Subject: WANTED: Bacteria/ Virus Detection Kit Date: 24 Oct 1996 15:52:42 -0700 Organization: America Online, Inc. (1-800-827-6364) Lines: 25 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <54ok27$m5o@newsbf02.news.aol.com> NNTP-Posting-Host: net.bio.net Wanted: We are seeking a kit for fast and simple bacterial detection. A major company is looking for test kits that can indicate the presence or absence of bacteria/ viruses on surfaces. Criteria: The method used must be simple, requiring no more than 4-5 steps or 2-3 reagents. The tests must also work fast, within 1 hour. Desired methods must indicate the presence of bacteria and viruses, either dead or alive. Ideally, the method will show organism activity. A simple self-contained kit is far preferrable to any method involving complex instrumentation. Funding for additional R&D may be available. Funding company would represent an ongoing captive market for newly commercialized test kits. Contact: Harold A. Meyer, III Chairman The Hook Appropriate Technology 52 Bank Street, Suite A New Milford, CT USA 06776-2706 TheHookTek@aol.com (or) tekscout@thehooktek.com From owner-biotechniques@net.bio.net Mon Oct 28 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Jeff James Newsgroups: bionet.journals.letters.biotechniques Subject: re: pipetman Date: 29 Oct 1996 06:21:30 -0800 Organization: University of Washington Lines: 9 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I am looking for a 96 well pipetman, I understand it may be called a 'splatt' pipetor, any help in locating this item would be greatly appreciated. thanks, jeff From owner-biotechniques@net.bio.net Thu Oct 31 22:00:00 1996 Path: biosci!biosci!not-for-mail From: lorraine@nature.berkeley.edu (Lorraine Sohlberg) Newsgroups: bionet.journals.letters.biotechniques Subject: Image manipulation guidelines Date: 1 Nov 1996 05:33:49 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <55cu7t$hba@net.bio.net> NNTP-Posting-Host: net.bio.net Bionetters- I work at a facility where researchers (myself included) video capture and scan-in images for formatting with Photoshop or Canvas to generate figures. I see a lot of image manipulation here and was wondering if there are any useful guidelines for this. Has anyone ever seen any published or unpublished guidelines outlining acceptable and unacceptable modifications of digital images (of gels, blots, in situs,cells,etc.) for scientific publication? Are there any guidelines for documenting the alterations done to an image before publication? Everyone seems to be using Photoshop to process their data these days and I was wondering if anyone has put down in black and white what is acceptable or not. Do you know of any imaging newsgroup where if I put out a query along these lines I would get some feedback? Would anyone like to propose any such guidelines? Inquiring minds want to know- Lorraine Sohlberg, NSF Center for Plant and Microbial Biology at UC Berkeley