From owner-biotechniques@net.bio.net Wed Dec 04 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Robert Gross Newsgroups: bionet.journals.letters.biotechniques Subject: apoptosis Date: 5 Dec 1996 06:53:46 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <586nlq$4gq@net.bio.net> NNTP-Posting-Host: net.bio.net I'm looking for 3 BT papers on nick end-labelling techniques, but my library doesn't carry BT. 1. Piqueras et al., BT, 20(4):634-40 (Apr., 1996) 2. Tornusciolo et al., BT, 19(5):800-5 (Nov., 1995) 3. Paper on Br-dUTP in July, 1996. If someone has access to these, and would be willing to fax me a copy, please e-mail me at robert.gross@utoronto.ca, and I will give you my fax number. Thanks. From owner-biotechniques@net.bio.net Thu Dec 12 22:00:00 1996 Path: biosci!biosci!not-for-mail From: brackena@tcd.ie (Adrian Bracken) Newsgroups: bionet.journals.letters.biotechniques Subject: Heat shock targets Date: 13 Dec 1996 05:18:13 -0800 Organization: Trinity College, Dublin 2 Lines: 4 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net If there is anybody out there involved in the field of Heat shock proteins and specifically their targets, ie what they actually interact with to repair stress damage", I am extremely interseted in discussing ideas. Pleas E-mail me at brackena@tcd.ie. Thank you. From owner-biotechniques@net.bio.net Thu Dec 12 22:00:00 1996 Path: biosci!biosci!not-for-mail From: =?UNKNOWN?Q?=B1=E2=BB=FD=C3=E6=C7=D0=B1=B3=BD=C7_=B1=B3=BC=F6_=C8?= Newsgroups: bionet.journals.letters.biotechniques Subject: Reamplification of the eluted PCR amplicons from PAGE gel Date: 13 Dec 1996 05:16:16 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <32A9CE59@yumc.yonsei.ac.kr> NNTP-Posting-Host: net.bio.net I am working on DDRT-PCR with amoebas. Currently, I found bunch of PCR products which showed differential display between compared mRNA populations. I eluted target amplicons from dried 6% polyacrylamide gel containing 7M urea according to Maniatis' protocol and tried to reamplify target DNA. Here, I am in trouble of reamplifying the target DNA of the same size in the original PAGE gel. I had modified the PCR conditions but I could not get the exact target amplicons. Sometimes I got smaller amplicons than the target, which were not present in the negative control reactions with no DNA. Please, help me to solve this problem. Thanks in advance. YPH From owner-biotechniques@net.bio.net Fri Dec 13 22:00:00 1996 Path: biosci!biosci!not-for-mail From: SacBunt96@gnn.com Newsgroups: bionet.journals.letters.biotechniques Subject: Hi Date: 13 Dec 1996 17:19:18 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <199612070350.WAA07754@mail-e2b.gnn.com> NNTP-Posting-Host: net.bio.net Hello! I'm a freshman in highschool and is in a honors bio class. I was wondering if you have any journals or know of any that are on the internet that contain the topic of "thermodynamics". I would gladly appreciate it. Thank you for your time. Future Scientist P.S. Please reply as quick as possible. From owner-biotechniques@net.bio.net Wed Dec 18 22:00:00 1996 Path: biosci!biosci!not-for-mail From: charles.lawrie@trinity.oxford.ac.uk (charles lawrie) Newsgroups: bionet.journals.letters.biotechniques Subject: Re:Biotechniques article Date: 19 Dec 1996 05:32:26 -0800 Organization: Oxford University Lines: 11 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <59bfkc$2s2@news.ox.ac.uk> NNTP-Posting-Host: net.bio.net Please help I am trying to track down an article from a 1989 BioTechniques volume 7 (10) pp 1096-1103 by Brenner, CA et al on the use of RT-PCR to amplify low quanties of mRNA .Does anybody have a copy of this article that they could send me I would be very grateful . Also I am trying to produce labbelled cDNA for use as a probe from a low quantity of mRNA producing insect material . Any suggestions would be very helpful . Thanks . Charles.lawrie@trinity.ox.ac.uk From owner-biotechniques@net.bio.net Thu Dec 19 22:00:00 1996 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.letters.biotechniques Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 20 Dec 1996 05:23:23 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <199612201000.CAA05200@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-biotechniques@net.bio.net Sat Dec 21 22:00:00 1996 Path: biosci!biosci!not-for-mail From: MMCCARTHY@biotechnet.com Newsgroups: bionet.journals.letters.biotechniques Subject: BioTechniques 22(1), January 1997 Date: 21 Dec 1996 18:33:22 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 180 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <01IDABL4QROY8Y6V0D@delphi.com> NNTP-Posting-Host: net.bio.net BioTechniques 22(1), January 1997 Table of Contents BENCHMARKS Nonradioactive method for quantitation of PCR products without hybridization with a specific probe -Atamas S.P. and B. White Screening for homologous recombination in ES cells using RT-PCR -Danoff T.M., M. Chiang, P.A. Janne and E.G. Neilson Simultaneous introduction of multiple mutations using overlap extension PCR -Ge L. and P. Rudolph Primer-directed mutagenesis of an intact plasmid by using Pwo DNA polymerase in long distance inverse PCR -Hidajat R. and P. McNicol Ligation of single adaptors allows efficient cloning of long PCR products -Ren S. and J.M. Ruppert Optimized conditions for cloning PCR products into an XcmI T-vector -Schutte B.C., K. Ranade, J. Pruessner =20 and N. Dracopoli Universal primers for sequencing DNA restriction fragments -Chen Y.J. and M.S. Valenzuela Improved method for isolation of DNA from slow-growing basidiomycetes such as Armillaria mellea -Flynn T.M. and W.G. Niehaus Enhancement of transformation in Pseudomonas aeruginosa PAO1 by magnesium and heat -Irani V.R. and J.J. Rowe Rapid method for cloning a calcium-dependent metalloprotease from a gram-positive bacterium in Escherichia coli -Kohl A., K.-D. Wittchen and F. =20 Meinhardt Simplified selection of transgenic Arabidopsis thaliana seed in liquid culture -Nichols K.W., G.R. Heck and D.E. =20 Fernandez Discrimination of run-on transcription from constitutive genes and antisense expression vectors in the same cells -Koropatnick J., J. DeMoor, M. Vincent =20 and O. Collins Mammalian expression vector with two multiple cloning sites for expression of two foreign genes -Tsang T.C., D.T. Harris, E.T. =20 Akporiaye, R.S. Chu, J. Brailey, F. Liu, F.H. Vasanwala, S.F. Schluter =20 and E.M. Hersh Site-directed mutagenesis for large insertions by oligonucleotide primers in optimized molar ratios -Wang D. and H.S. Sul Reduction of background in protein kinase assays by electroblotting -Enke D.A. and M.J. Solomon Removal of an inhibitor of marker enzyme activity in artery extracts by chelating agents -Oswald H., F. Heinemann, S. Nikol, B. =20 Salmons and W.H. G=81nzburg Fabrication of an autoclavable one-way valve for venting fermentation gases -Skory C.D. and H.A. Wyckoff Rapid purification of microtubule motor domain proteins expressed in bacteria -Song H. and S.A. Endow Method for simultaneous RNA and DNA isolation =66rom biopsy material, culture cells, plants and bacteria -Dobbeling U., R. Boni, A. Haffner, R. =20 Dummer and G. Burg THE INTERNET ON-RAMP -Cyberspace for Biologists SHORT TECHNICAL REPORTS Immunobead RT-PCR: a sensitive method for detection of circulating tumor cells -Eaton M.C., J.E. Hardingham, D. Kotasek and A. Dobrovic Deoxyribonuclease treatment improves the homogeneity of single-stranded DNA preparations -Aliev T.K., A.A. Panina, V.G. Korobko =20 and S.D. Varfolomeyev Relationship between opacity of transformed E. coli colonies and over-expression of the recombinant transcript -Barik S. Methods and applications of three color confocal imaging -Paddock S.W., E.J. Hazen and P.J. =20 DeVries BIOFEATURE Continuous fluorescence monitoring of rapid cycle DNA amplification -Wittwer C.T., M.G. Herrman, A.A. Moss =20 and R.P. Rasmussen RESEARCH REPORTS Specific detection of His-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions -Lindner P., K. Bauer, A. Krebber, L. =20 Nieba, E. Kremmer, C. Krebber, A. =20 Honegger, B. Klinger, R. Mocikat and A. Pluckthun Use of dicistronic expression cassette encoding the green fluorescent protein for the screening and selection of cells expressing inducible gene products -Mosser D.D., A.W. Caron, L. Bourget, P. Jolicoeur and B. Massie Tracking and quantitation of retroviral mediated transfer using a completely humanized, red shifted green fluorescent protein gene -Muldoon R.R., J.P. Levy, S.R. Kain, =20 P.A. Kitts and C.J. Link, Jr. PRODUCT APPLICATION FOCUS Analysis of differential display RT-PCR products using fluorescent primers and GeneScanTM software -Luehrsen K.R., L.L. Marr, E. van der =20 Knaap and S. Cumberledge The LightCyclerTM: A microvolume multisample fluorimeter with rapid temperature control -Wittwer C.T., K.M. Ririe, R.V. Andrew, D.A. David, R.A. Gundry and U.J. Balis NEW PRODUCTS INDEX TO ADVERTISERS From owner-biotechniques@net.bio.net Tue Dec 31 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Xiao Zhang Newsgroups: bionet.journals.letters.biotechniques Subject: IL-6 oligo sequence Date: 1 Jan 1997 15:28:13 -0800 Organization: GDMC Lines: 12 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <5abb5f$ge3@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net Hi,everyone I get a abstract from Medline,I want to know the sequence of the deoxyoligonucleotides complementary to the IL-6 mRNA, but this journal(J-Bone-Miner-Research, 1994 May; 9(5):753-7)is unavailable in our college, and difficult in other city in China. Would you like to tell me the oligonucleotide sequence used in this paper. Xiao Zhang Fax:86-759-2284104