From owner-biotechniques@net.bio.net Sat Feb 01 22:00:00 1997 Path: biosci!biosci!not-for-mail From: "C. Cantina" Newsgroups: bionet.journals.letters.biotechniques Subject: Starting Cancer Research - Looking for Publications Date: 2 Feb 1997 11:13:43 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <32F538E6.1981@iafrica.com> NNTP-Posting-Host: net.bio.net Hi, I am a final year BSc student (Biochemistry, Microbiology, and Botany), starting a cancer research project and need some help in finding the following publications: 1. McLAUGHLIN, J. L. 1991, Crown Gall Tumours on potato discs and Brine Shrimp Lethality: Two Simple Bioassays for Higher Plant Screeninng and Fractionations: In Methods in Plant Biochemistry 6. Academic Press, 1-32. 2. GEORG WALLER & KAZUO YAMASAKI, Advances in Experimental Medicine and biology, Vol. 403 & 404. The URL for this publication should be http://3y.pharm.hiroshim-u.ac.jp, but it does not work. Could someone please send me these publications, or tell me where I can find them. Should anyone have information about other sites/publications related to the above, please let me know! Please contact me under my E-mail address; ccantina@iafrica.com Thanks very much in advance C. Cantina PS. please forward this message to other relevant Newsgroups, in case I have left some out, thanks. From owner-biotechniques@net.bio.net Sun Feb 09 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Pradeep M Joshi Newsgroups: bionet.journals.letters.biotechniques Subject: sticky surfaces Date: 10 Feb 1997 05:18:01 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <5dmqph$d4a@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net hi! I am a new subscriber to the news group. I have run into a small problem. I want to do some surface staining of HaCat, an epithelial cell line for a FACS scan. The Problem now is that my cells dissociate only in th presence of trypsin and consequently most of my cell surface markers , receptors get chewed up. could you suggest me some way of getting a single cell suspension with my cell surface molecules intact for a FACS scan. Thanks, Pradeep From owner-biotechniques@net.bio.net Thu Feb 13 22:00:00 1997 Path: biosci!biosci!not-for-mail From: MMCCARTHY@biotechnet.com Newsgroups: bionet.journals.letters.biotechniques Subject: BioTechniques 22(3), March 1997 Date: 14 Feb 1997 13:25:39 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 197 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <01IFEUZ6TJ3E9D9RWL@delphi.com> NNTP-Posting-Host: net.bio.net BioTechniques 22(3), March 1997 Table of Contents BENCHMARKS Rapid plasmid minipreps in microplate format Mohler W.A. and H.M. Blau Method of extracting genomic DNA from non-germinated gymnosperm and angiosperm pollen Simel E.J., L.R. Saidak and G.A. Tuskan Enhanced DNA extraction and PCR amplification of mitochondrial genes from formalin-fixed museum specimens Shedlock A.M., M.G. Haygood, T.W. Pietsch and P. Bentzen Preparation of bone samples for DNA extraction: a nuts and bolts approach Thomas M.G. and L.J. Moore Plasmid mini-preparations from culture streaks Good L. and R.N. Nazar Preparation of RNA from Ruminococcus albus Nagamine T., R.I. Aminov, M. Suguira, K.Ogata, K. Tajima and Y. Benno Tissue homogenization as a key step in extracting RNA from human and rat pancreatic tissue Sparmann G., A. Jaschke, M. oehr, S. Liebe and J. Emmrich "Colony Sequencing": direct sequencing of plasmid DNA from bacterial colonies Kilger C., M. Krings, H. Poinar and S.Paabo Fast plasmid DNA sequencing using a thermal cycler and high temperature alkali denaturation Martsen E.O., M. Zeng and J.-N. Lapeyre Improved resolution of asymmetric-PCR SSCP products Pokorny R.M., A.B. Dietz, S. Galandiuk and H.L. Neibergs Protocol for site-directed mutagenesis in the preparation of large gene targeting vectors De Togni P. Method of site-directed mutagenesis using long primer-unique site elimination and exonuclease III Nicolas G., S. Pedroni, C. Fournier, H.Gautero and M.-C. Lecomte Scanning mutagenesis to localize DNA-protein interactions Rosmarin A.G., D.G. Caprio and C.P.Simkevich "Megaprimer" method of PCR-based mutagenesis: the concentration of megaprimer is a critical factor Smith A.M. and K.P. Klugman Quantitative RT-PCR for human Fas (CD95) expression Mitra D. and J. Laurence Nonradioactive PCR-SSCP assay to detect apoB Arg3500 to Gln mutation using a minigel format Hennessy L.K. and C.R. Pullinger Titering replication-defective virus for use in gene transfer Strayer D.S., L.-X. Duan, I. Ozaki, J. Milano, L.E. Bobraski and O. Bagasra Recovery of polypeptides cleaved from purified calmodulin-binding peptide fusion proteins Vaillancourt P., T.G. Simcox and C.-F. Zheng Measurement of antigen by enhanced chemiluminescent Western blot Huang D. and S.A. Amero Reusing hybridization mixtures Quigley N.B. Enhanced digital imaging of diaminobenzidene-stained immunoblots Smejkal G.B. and J.R. Shainoff THE INTERNET ON-RAMP Cyberspace for Biologists SHORT TECHNICAL REPORTS Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity Wilfinger W.W., K. Mackey and P.Chomczynski PCR-based method for isolation of full-length clones and splice variants from cDNA libraries Alphey L. Cryostat tissue printing: an improved method for histochemical and immunocytochemical localization in soft tissues Conley C.A. and M.R. Hanson Electromechanical microinjection of pink bollworm Pectinophora gossypiella embryos increases survival Peloquin J.J., S.T. Thibault, L.P.J. Schouest and T.A. Miller Sensitive, nonradioactive dot blot/ribonuclease protection assay for quantitative determination of mRNA Zhan J., H.D. Fahimi and A. Voekl RESEARCH REPORTS Rapid, universal method to isolate PCR-ready DNA using magnetic beads Rudi K., M. Kroken, O. Dahlberg, A. Deggerdal, K.S. Jakobsen and F. Larsen Quantification of genetically-tagged cyanobacteria in Baltic Sea sediment by competitive PCR Moller A. and J.K. Jansson Multiplex mutagenically separated PCR: Diagnosis of beta-thalassemia and hemoglobin variants Chang J.-G., H.-J. Liu, J.-M. Huang, T.-Y. Yang and C.-P. Chang Low-cost system for real-time monitoring of luciferase gene expression Gailey P.C., E.J. Miller and G.D. Griffin Generation of multiple mRNA fingerprints using fluorescence-based differential display and an automated DNA sequencer Jones S.W., D. Cai, O.S. Weislow and B. Esmaeli-Azad Preparation of animals with a high degree of chimerism by one step co-culture of embryonic stem cells and preimplantation embryos Khillan J.S. and Y. Bao PRODUCT APPLICATION FOCUS DNAzol, a reagent for the rapid isolation of genomic DNA Chomczynski P., C. Mackey, R. Drews and W. Wilfinger Rapid isolation of PCR-ready DNA from blood, bone marrow and cultured cells, based on paramagnetic beads Deggerdal A. and F. Larsen LITERATURE EXPRESS NEW PRODUCTS INDEX TO ADVERTISERS From owner-biotechniques@net.bio.net Wed Feb 19 22:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.letters.biotechniques Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 20 Feb 1997 06:31:17 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <199702201000.CAA20194@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net