From owner-biotechniques@net.bio.net Mon Mar 03 22:00:00 1997 Path: biosci!biosci!not-for-mail From: John Brennand Newsgroups: bionet.journals.letters.biotechniques Subject: Re: 96-well amino coated microtitre plates Date: 4 Mar 1997 13:42:48 -0800 Organization: Zeneca Pharmaceuticals Lines: 6 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <331C4308.2F11@gbapr.zeneca.com> References: NNTP-Posting-Host: net.bio.net derek costar (01494 471207) have a range of plates with different surface chemistrys. john From owner-biotechniques@net.bio.net Mon Mar 03 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Mail@scri.sari.ac.uk Newsgroups: bionet.journals.letters.biotechniques Subject: 96-well amino coated microtitre plates Date: 4 Mar 1997 05:54:58 -0800 Organization: Scottish Crop Research Institute Lines: 10 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hello I am looking for a supplier of 96-well amino coated microtitre plates. Can any one help? Cheers Dr Derek Stewart d.stewart@scri.sari.ac.uk From owner-biotechniques@net.bio.net Mon Mar 03 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Keld Sorensen Newsgroups: bionet.journals.letters.biotechniques Subject: Re: 96-well amino coated microtitre plates Date: 4 Mar 1997 13:44:32 -0800 Organization: University of Illinois, College of Medicine Lines: 11 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <331BFE4D.5B58@uic.edu> References: NNTP-Posting-Host: net.bio.net Nunc used to carry plates coated with secondary amines. Lately I have received a flyer saying that they now carry ones coated with primary amines which theoretically should be advantageous. Corning-Costar carries various plates (NHS coated, i.e. NH2 reactive), COOH, and some additional ones. Pierce carries a long list of "activated" and "coated" plates maleic anhydride (NH2 reactive), NeutrAvidin, Streptavidin, Protein A, Protein G, Goat anti Mouse, Glutathione, Metal Chelate, Dextrin and more. From owner-biotechniques@net.bio.net Thu Mar 13 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Neuronal Virus Newsgroups: bionet.journals.letters.biotechniques Subject: How can I solubilize my protein for FPLC injection? Date: 14 Mar 1997 05:37:19 -0800 Organization: Dondamoa Co., Ltd. Lines: 34 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <01bc3025$606cd680$2d382e93@ercc.snu.ac.kr> NNTP-Posting-Host: net.bio.net Keywords: Protein solubilizing This is a multi-part message in MIME format. ------=_NextPart_000_01BC3070.D08E7A40 Content-Type: text/plain; charset=EUC-KR Content-Transfer-Encoding: 7bit I am trying to solubilize my protein which was desalted and lyophilized for further purification by FPLC. But it defied to be solved in small amount of solvent (e.g., water). Injection solution should be, as you well know, as small amount as possible. Maybe the protein has hydrophobic patches outside and aggregates each other or its concentration is too high to be dissolved in. But whenever I diluted it enough, its peak was hard to recognize. Have you experienced this type of dilemma? Any suggestions and opinions will be a great help! ------=_NextPart_000_01BC3070.D08E7A40 Content-Type: text/html; charset=ISO-2022-KR Content-Transfer-Encoding: quoted-printable =1B$)C

I am trying to solubilize my protein = which was desalted and lyophilized for further purification by = FPLC.
But it defied to be solved in small amount of solvent (e.g., = water). Injection solution should be, as you well know, as small = amount as possible.
Maybe the protein has hydrophobic patches = outside and aggregates each other or its concentration is too high to be = dissolved in. But whenever I diluted it enough, its peak was hard = to recognize. Have you experienced this type of dilemma?
Any = suggestions and opinions will be a great help!

------=_NextPart_000_01BC3070.D08E7A40-- From owner-biotechniques@net.bio.net Fri Mar 14 22:00:00 1997 Path: biosci!biosci!not-for-mail From: MMCCARTHY@biotechnet.com Newsgroups: bionet.journals.letters.biotechniques Subject: BioTechniques 22(4), April 1997 Date: 15 Mar 1997 09:30:13 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 177 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <01IGJ55UKG829ZO042@delphi.com> NNTP-Posting-Host: net.bio.net BioTechniques 22(4), April 1997 Table of Contents BENCHMARKS Chemiluminescent detection of protein molecular weight markers in Western blot techniques Liu R.H., J. Jacob and B. Tennant Southern blotting of long-term preserved DNA Laniel M.-A., M. El-Amine, G. Boire and H.-A. Menard Low-power vacuum apparatus for blotting Passey R.J. Increased 32P-SSCP sensitivity by combining restriction enzyme-digestion and extended X-ray film exposures Lovlie R. and H.G. Eiken Separation of DNA strands facilitates detection of point mutations by PCR-SSCP Selvakumar N., B.-C. Ding and S.M. Wilson Improved resolution of asymmetric-PCR SSCP products Pokorny R.M., A.B. Dietz, S. Galandiuk and H.L. Neibergs DNA template as a source of artifact in the detection of p53 gene mutations using archived tissue Shiao Y.-H., G.S. Buzard, C.M. Weghorst and J.M. Rice Easy method for the cloning of mammalian cell colonies Thompson J.A. and G. Ioli Two-layer gradient isolation of rat peritoneal mast cells Price J.A. Micropreparation of cultured cells for in situ reverse transcription PCR Zhang H. and S. Wadler Site-directed mutagenesis using a PCR-based staggered re-annealing method without restriction enzymes Ailenberg M. and M. Silverman Semiquantitative RT-PCR: Enhancement of assay accuracy and reproducibility Saric T. and S.A. Shain Direct PCR from paraffin-embedded tissue Burns W.C., Y.S. Liu, C. Dow, R.J.S. Thomas and W.A. Phillips Bandstab: a PCR-based alternative to cloning PCR products Wilton S., L. Lim, D. Dye and N. Laing Sequential extraction of DNA and DNA-binding proteins from low cell numbers El-Osta S., P. Kantharidis and J.R. Zalcberg Non-radioisotopic identification of endonucleases involved in apoptosis Boone D.L. and B.K. Tsang Easy gene walking Harrison R.W., J.C. Miller, M.J. D'Souza and G. Kampo Automated fluorescent DNA sequencing by a simplified solid-phase chemical sequencing method Ohara R., A. Tanaka and O. Ohara Microassay for the assessment of low levels of hydroxyproline Creemers L.B., D.C. Jansen, A. van Veen-Reurings, T. van den Bos and V. Everts THE INTERNET ON-RAMP Cyberspace for Biologists SHORT TECHNICAL REPORTS Northern blotting of RNA denatured in glyoxal without buffer recirculation Burnett W.V. Identification of recombinant baculoviruses using green fluorescent protein as a selectable marker Wilson L.E., N. Wilkinson, S.A. Marlow, R.D. Possee and L.A. King Determination of human NAT2 acetylator genotype by oligonucleotide ligation assay Bigler J., C. Chen and J.D. Potter Detection of DNA polymorphisms by fluorescent RAPD analysis Corley-Smith G.E., C.J. Lim, G.B. Kalmar and B.P. Brandhorst Quantitative analysis of 16S rDNA using competitive PCR and the QPCR System 5000 Blok H.J., A.M. Gohlke and A.D.L. Akkermans Analysis of 3H-proline-labeled protein by rapid filtration in multiwell plates for the study of collagen metabolism Koyano Y., H. Hammerle and J. Mollenhauer RESEARCH REPORTS Recombinant HMG1 protein produced in Pichia pastoris: a non-viral gene delivery agent Mistry A.R., L. Falciola, L. Monaco, R. Tagliabue, G. Acerbis, A. Knight, R.P. Harbottle, M. Soria, M.E. Bianchi, C. Coutelle and S.L. Hart Expression of foreign proteins on the surface of Autographa californica nuclear polyhedrosis virus Grabherr R., W. Ernst, O. Doblhoff-Dier, M. Sara and H. Katinger High sensitivity of laser-induced fluorescence detection in capillary gel electrophoresis for accurate apolipoprotein E-genotyping Schlenk A., K. Bohnet, D. Aguillon, C. Lafaurie, G. Siest and S. Visvikis Heat-mediated activation of affinity-immobilized Taq DNA polymerase Nilsson J., M. Bosnes, F. Larsen, P.-A. Nygren, M. Uhlen and J. Lundeberg PRODUCT APPLICATION FOCUS Rainbow Universal CPG: a versatile solid support for oligonucleotide synthesis Nelson P.S., S. Muthini, M. Vierra, L. Acosta and T.H. Smith ThermoSequenase DNA Polymerase and T. acidophilum pyrophosphatase: New thermostable enzymes for DNA sequencing Vander Horn P.B., M.C. Davis, J.J. Cunniff, C. Ruan, B.F. McArdle, S.B. Samols, J. Szasz, G. Hu, K.M. Hujer, S.T. Domke, S.R. Brummet, R.B. Moffett and C.W. Fuller NEW PRODUCTS INDEX TO ADVERTISERS From owner-biotechniques@net.bio.net Mon Mar 17 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Jan Marten de Jong Newsgroups: bionet.journals.letters.biotechniques Subject: philosophy paper Date: 18 Mar 1997 06:23:01 -0800 Organization: Universiteit Twente Lines: 22 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <01bc32dd$24ae5fa0$8fe35982@cal010054.student.utwente.nl> NNTP-Posting-Host: net.bio.net L.S., My name is Jan Marten de Jong. I'm a student of the university of Twente (netherlands) and I'm studying applied communications. This trimester I have to make an essay for Philosophy. I'd like to do it about bio-technology. More specificly, about identification (DNA fingerprinting for crime, insurance issues, screening of large populations, etc.) Now I wondered if maybe anyone had written about this subject. Maybe there is some information on the net about it. Or someone has an article about it. It doesn't matter what it is, I can use (almost) everything. If you have something for me, you can mail me (j.m.dejong@student.utwente.nl) or you can post it in this newsgroup. Thanks already! Jan Marten de Jong From owner-biotechniques@net.bio.net Wed Mar 19 22:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.letters.biotechniques Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 20 Mar 1997 06:00:46 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <199703201000.CAA12764@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-biotechniques@net.bio.net Thu Mar 27 22:00:00 1997 Path: biosci!biosci!not-for-mail From: John Brandt Newsgroups: bionet.journals.letters.biotechniques Subject: Non-radioactive labelling Date: 28 Mar 1997 13:55:39 -0800 Organization: National Genetics Institute Lines: 6 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <333C0C00.6BC3@ngi.com> NNTP-Posting-Host: net.bio.net I currently use digoxigenin-labelled probes and a colorimetric detection assay using anti-DIG antibodies (NBT/BCIP). I want to incorporate fluorescent detection. I would like feedback on anti-DIG-fluorescein, -rhodamine, -AMCA, or other detection methods. Thank you, in advance, for any suggestions. From owner-biotechniques@net.bio.net Mon Mar 31 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Rolf Kocherhans Newsgroups: bionet.journals.letters.biotechniques Subject: Free molbio prg. available here ! Date: 1 Apr 1997 05:37:21 -0800 Organization: University of Zurich Lines: 42 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <333FD5CC.474@vetvir.unizh.ch> NNTP-Posting-Host: net.bio.net Hi there I am presently looking for molecular biologists willing to test some programs I made aimed to speeding up the daily lab work ! - This is how it works: The programs are accessible over the WWW, all you have to do is download a free plugin to enhance the browser you are using (Netscape 2.x or Internet Explorer 2.x or higher) on your Mac or Windows computer ! - What you have to do first: Download the appropriate plugin and install it on your computer, here is the URL: http://www.unizh.ch/vetvir/plugin.html - Then connect to: http://www.unizh.ch/vetvir/programs.html These are the programs which make your live as a molecular biologist easier ! a. Digest Preview enter the size(s) of your DNA fragment(s) and see their migration pattern in a virtual gel in comparison to a 1 kb ladder. b. Dilution Calculator does all the calculations when you have to make up solutions. c. Adaptor Design helps to create adaptors in order to link incompatible DNA ends together,in frame ! there many others: Oligo Tm,Compatible ends etc. Please have a look and comment ! Regards Rolf Kocherhans mailto:rolfk@vetvir.unizh.ch