From owner-biotechniques@net.bio.net Tue Jul 01 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Dr. K. P. Chepenik" Newsgroups: bionet.journals.letters.biotechniques Subject: Caltag biotin kit Date: 2 Jul 1997 14:02:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <5pefke$cp9@net.bio.net> NNTP-Posting-Host: net.bio.net Hello, Has anyone out there had any experience with Caltag's ANNEXINV15 Biotin kit, particularly as applied to tissues of the central nervous system? Thank you for your reply. Ken Chepenik From owner-biotechniques@net.bio.net Sun Jul 13 23:00:00 1997 Path: biosci!biosci!not-for-mail From: tina@borcim.wustl.edu (Tina Myers) Newsgroups: bionet.journals.letters.biotechniques Subject: Glyoxal gels Date: 14 Jul 1997 08:04:23 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <199707141441.JAA01525@borcim.wustl.edu> NNTP-Posting-Host: net.bio.net Hello, Our laboratory has recently adopted the method of RNA denaturation in glyoxal without running buffer for both northern blotting and direct examination of the radiolabeled RNA. While the signal obtained following northern blot is satisfactory, the high nonspecific background observed after even short exposures of the dried gel precludes visualization of the 32P-labeled RNA transcription products. We have tried washing the gel in dH2O prior to fixing without success. Examination of the RNA on a UV transilluminator shows that the RNA is a sharply, defined band, without degradation. Has anyone tried this methodology, which was in volume 22, number 4, pp 668-671, 1997 of Biotechniquesfor direct examination of radiolabeled products? We are currently testing different washing procedures to see if that will help. Thank you, Tina Myers email: tina@borcim.wustl.edu From owner-biotechniques@net.bio.net Sun Jul 20 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Michele Markstein Newsgroups: bionet.journals.letters.biotechniques Subject: Protein Transfection Date: 21 Jul 1997 11:58:39 -0700 Organization: University of Chicago Lines: 16 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <33CF839B.64B1@midway.uchicago.edu> NNTP-Posting-Host: net.bio.net I would like to transfect yeast cells with proteins, and would appreciate any information regarding protein transfection (in any cell type). Becasue the yeast cell wall may be an obstacle, my first approach will be to fuse liposomes to spheroplasted yeast cells. Therefore, I am also intereseted in general inights/information about yeast lipofection. In case you are wondering why I am transfecting proteins instead of DNA, my project is to study the effects of prions--a protein based phenomenon. Thanks for your time. Sincerely, Michele Markstein From owner-biotechniques@net.bio.net Sun Jul 20 23:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.letters.biotechniques Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 21 Jul 1997 11:59:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <199707200900.CAA06346@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-biotechniques@net.bio.net Wed Jul 23 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Ben Williams Newsgroups: bionet.journals.letters.biotechniques Subject: Forum Date: 24 Jul 1997 06:53:46 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I wonder if anyone can point me in the direction of a forum for the discussion of the issues surrounding genetic modificaation etc? Any help appreciated - and sorry to crash the conference like this. Ben -- "Curiouser and curiouser!" cried Alice. - Lewis Carroll Ben Williams From owner-biotechniques@net.bio.net Sat Jul 26 23:00:00 1997 Path: biosci!biosci!not-for-mail From: MMCCARTHY@biotechnet.com Newsgroups: bionet.journals.letters.biotechniques Subject: BioTechniques 23(2), August 1997 Date: 26 Jul 1997 19:27:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 155 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <01ILPITUE3ZM9ODFGB@delphi.com> NNTP-Posting-Host: net.bio.net BioTechniques 23(2), August 1997 Table of Contents Benchmarks In Situ RT-PCR Using Fluorescence-Labeled Primers U. Stein, W. Walther, J. Wendt and T.A. Schild Purification and Cloning of Differential Display Products S. Gery and S. Lavi PCR Synthesis of cDNA from Total RNA S.B. Shepard and A.G. Cooper Pitfalls of PCR: Cross-Reactivity with Joyride E. coli Nucleic Acid M. Kenzelmann and K. Muhlemann Rapid Cloning of PCR-Derived RAPD Probes A.M. Comes, J.F. Humbert and F. Laurent PCR-Based Multiplex Method for Rapid Screening of Recombinant Bacteria S. Schillberg, D. Schumann and R. Fischer Direct Sequencing of PCR-Amplified 23S rDNA A. Ibrahim and A. Sjostedt Method to Reverse the Order of Multiple Cloning Sites C.-S. Lin Immediate Digestion of Fish Muscle Following Field Collections Yields DNA Suitable for RAPD Fingerprinting J.S. Nelson, C.W. Khiong, C.L. Ming and V.P.E. Phang Isolation of DNA Suitable for PCR for Field and Laboratory Work J. Altschmied, U. Hornung, I. Schlupp, J. Gadau, R. Kolb and M. Schartl Use of a Dental Amalgamator to Extract RNA from the Gram-Positive Bacterium Streptococcus agalactiae H.H. Yim and C.E. Rubens Quality Control of Centrifugal Elutriation for Studies of Cell Cycle Regulation M. Hengstschlager, O. Pusch, T. Soucek,E., Hengstschlager-Ottnad and G. Bernaschek Nonselective URA3 Colony-Color Assay in Yeast ade1 or ade2 Mutants Y.-s. Weng and J.A. Nickoloff Rapid Generation of DNA Probes by Amplification of Tandem Repeats G. Trendelenburg and C. Hanski Pyrophosphate in the Binding Reaction Increases the Sensitivity of Mobility Shift Analysis C. Fenton and M. Raafat El-Gewely Re-probing of Immunoblots after Storage for More than a Decade S.-U. Gorr Universal Template Plasmid for Introduction of the Triple-HA Epitope Sequence into Cloned Genes M.H. Sato and Y. Wada The Internet On-Ramp Cyberspace for Biologists Short Technical Reports Photoactivation of Caged Compounds in Single Living Cells: An Application to the Study of Cell Locomotion A. Ishihara, K. Gee, S. Schwartz, K. Jacobson and J. Lee Automated Differential Display Using a Fluorescently Labeled Universal Primer N.R. Smith, M. Aldersley, A. Li, A.S. High, T.P. Moynihan, A.F. Markham and P.A. Robinson Use of Internal Controls to Increase Quantitative Capabilities of the Ribonuclease Protection Assay M.J.J. Davis, C.S. Bailey and C.K. Smith, II Increased Informativeness of RAPD Analysis by Detection of Microsatellite Motifs J. Ramser, K. Weising, V. Chikaleke and G. Kahl Efficient Cloning Method that Selects the Recombinant Clones E. Loukianov, T. Loukianova and M. Periasamy Identification of Endonuclease Activity in HIV-1 gp120 Preparations Produced Using Baculovirus Expression Systems A.N. Davidoff and B.V. Mendelow Long-Range and Highly Sensitive DNase I Footprinting by an Automated Infrared DNA Sequencer M. Machida, H. Kamio and D. Sorensen Research Reports Generation of Large Libraries of Random Mutants in Bacillus subtilis by PCR-Based Plasmid Multimerization S. Shafikhani, R.A. Siegel, E. Ferrari and V. Schellenberger Automated Cycle Sequencing with Taquenase: Protocols for Internal Labeling, Dye Primer and "Doublex" Simultaneous Sequencing H. Voss, U. Nentwich, S. Duthie, S. Wiemann, V. Benes, J. Zimmermann and W. Ansorge Product Application Focus Self-Seal Reagent: Evaporation Control for Molecular Histology Procedures without Chambers, Clips or Fingernail Polish D.E. Sullivan, L.E. Bobroski, O. Bagasra and M. Finney New Products Index to Advertisers From owner-biotechniques@net.bio.net Sat Jul 26 23:00:00 1997 Path: biosci!biosci!not-for-mail From: RHODES@UCONNVM.UCONN.EDU Newsgroups: bionet.journals.letters.biotechniques Subject: 96 well plates ?? Date: 26 Jul 1997 19:38:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <5recae$51@net.bio.net> NNTP-Posting-Host: net.bio.net Does anyone know of a source for 96 well (or 6 or 12 or 24 or 384...) plates made of something OTHER than plastic? Thanks! | O==O | | David G. Rhodes O==O Phone 860-486-5413 | | School of Pharmacy; U-92 O==O Fax 860-486-4998 | | University of Connecticut O==O | | Storrs, CT 06269-2092 O==O rhodes@uconnvm.uconn.edu | |_____________________________O==O____________________________| CAUTION: Dates on calendar are closer than they appear!