From owner-biotechniques@net.bio.net Wed Oct 01 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Florence et =?UNKNOWN?Q?R=E9my?= Longueville Newsgroups: bionet.journals.letters.biotechniques Subject: Silver amplification protocole Date: 2 Oct 1997 13:47:23 -0700 Organization: Grolier Interactive Europe Lines: 10 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <3433C19F.5B9CB823@club-internet.fr> NNTP-Posting-Host: net.bio.net I'm a PhD student in neuroscience and I'm looking for information (protocole and/or ref.) about silver amplification used in immunohistochemistry or in immunocytochemistry. I would like to be able to distinguish the double labeling of spinal cord section for noradrenergic positive fibers and for WGA-HRP retrogradely labeled neurones. I suppose that sylver enhancement of the noradrenergic immunopositive fibers could be done. Thanks for answering to remyflo@club-internet.fr From owner-biotechniques@net.bio.net Sun Oct 05 23:00:00 1997 Path: biosci!biosci!not-for-mail From: zx Newsgroups: bionet.journals.letters.biotechniques Subject: tetracycline conjugation Date: 6 Oct 1997 03:42:40 -0700 Organization: GDMC Lines: 14 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <615k2i$cr0@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net Hi All, Would u like to tell me how to conjugate a tetracycline to a protein, for example, BSA? please tell me the method and related paper. Thank you in advance ! zhangx zjzxlhrm@pub.zhanjiang.gd.cn From owner-biotechniques@net.bio.net Wed Oct 15 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Beyene Lulseged Newsgroups: bionet.journals.letters.biotechniques Subject: Prestaining proteins Date: 16 Oct 1997 06:26:06 -0700 Organization: San Jose State University Lines: 7 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <34453D21.6408@isc.sjsu.edu> NNTP-Posting-Host: net.bio.net I would like to make my own prestained protein standards for SDS PAGE, similar to those sold by various companies (BioRad, Sigma, etc.) The companies are unwilling to share their techniques. Does anyone have a reference or method? J. Shackelford, San Diego State University jshackel@sunstroke.sdsu.edu From owner-biotechniques@net.bio.net Mon Oct 20 23:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.letters.biotechniques Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 21 Oct 1997 06:30:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <199710200900.CAA23218@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. 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If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. 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From owner-biotechniques@net.bio.net Tue Oct 21 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Simon Firek Newsgroups: bionet.journals.letters.biotechniques Subject: text book on gene manipulation Date: 22 Oct 1997 11:31:21 -0700 Organization: University of Wales, Aberystwyth Lines: 9 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <344E1220.2228@aber.ac.uk> NNTP-Posting-Host: net.bio.net I need to teach a 3rd year undegraduate course on the principles of gene manipulation e.g. cloning, sequencing, PCR etc. Could anyone recommend a good up to date text book which would cover this area. Thanks, Simon From owner-biotechniques@net.bio.net Tue Oct 21 23:00:00 1997 Path: biosci!biosci!not-for-mail From: MMCCARTHY@biotechnet.com Newsgroups: bionet.journals.letters.biotechniques Subject: BioTechniques 23(5), November 1997 Date: 22 Oct 1997 13:42:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 173 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <01IP3YBK9C7692YHIB@delphi.com> NNTP-Posting-Host: net.bio.net BioTechniques 23(5), November 1997 Table of Contents BENCHMARKS Preparative Electrophoresis: An Improved Method for the Isolation of Human Recombinant Apolipoprotein A-I H.H.-J. Schmidt, J. Genschel, R. Haas and M.P. Manns Method to Reduce the Quantity of Ethidium Bromide Required to Stain DNA in Agarose Gels M.J. Lucey, S.M. McColl and F.C.R. Manning Simplification of Titer Determination for Recombinant Baculovirus by Green Fluorescent Protein Marker H.J. Cha, T. Gotoh and W.E. Bentley Serum-Free Production, Concentration and Purification of Recombinant Retroviruses J. Seppen, R.J. Kimmel and W.R.A. Osborne Semiquantitative Comparison of the DNA-Binding Activity of In Vitro-Synthesized Proteins P. Chene Using a Eukaryotic GST Fusion Vector for Proteins Difficult to Express in E. coli R.Y.L. Tsai and R.R. Reed Production and Refolding of Recombinant Leptin P.L. Zamorano, L. De Sevilla, V.B. Mahesh and D.W. Brann Manipulation of Transcription Factor Activity Using Fluorescein-Tagged Dumbbell Oligonucleotides M. Steinmayr, J.-P. Aubry and M. Becker-Andre Cosmid Vector for the Cloning and Study of Complex GC-Rich Genomes S. Ananvoranich and C. Simard Duplication of a Region in the Multiple Cloning Site of a Plasmid Vector to Enhance Cloning-Mediated Addition of Restriction Sites to a DNA Fragment L. Cocea Stable DNA-Binding Yeast Vector Allowing High-Bait Expression for Use in the Two-Hybrid System O. Louvet, F. Doignon and M. Crouzet High-Efficiency T-Vector Cloning of PCR Products by Forced A-Tagging and Post-Ligation Restriction Enzyme Digestion H. Bielefeldt-Ohmann and D.R. Fitzpatrick PCR-Generated Crossover Linkers for Site-Directed Mutagenesis A.C. Boyd and D.J. Porteous Site-Specific Mutagenesis of Immunoglobulin Domains by Multiple-Fragment Homologous Recombination M. Eghtedarzadeh-Kondri, M.A. Walls and S.M. Glaser In a Blinded Analysis, Restriction Endonuclease Fingerprinting Detects All the Mutations in a 1.9-kb Segment Q. Liu, J. Feng and S.S. Sommer Nonradioactive Method for the Determination of Internucleosomal Cleavage Associated with Apoptosis C.-F. Wu, N.H. Bishopric and R.E. Pratt Reliable Detection of DNA CpG Methylation Profiles by the Isoschizomers MspI/HpaII Using Oligonucleotide Stimulators D. Kupper, M. Reuter, A. Meisel and D.H. Kruger THE INTERNET ON-RAMP Cyberspace for Biologists SHORT TECHNICAL REPORTS Cloning and Assembly of PCR Products Using Modified Primers and DNA Repair Enzymes D.E. Watson and G.N. Bennett Application of a Chimeric Green Fluorescent Protein to Study Protein-Protein Interactions N. Garamszegi, Z.P. Garamszegi, M.S. Rogers, S.J. DeMarco and E.E. Strehler Dicistronic LacZ and Alkaline Phosphatase Reporter Constructs Permit Simultaneous Histological Analysis of Expression from Multiple Transgenes X. Li, W. Wang and T. Lufkin Detection of False-Negative Results in Nested Primer PCR of Proviral HIV-1 DNA K. Zimmermann, B. Plaimauer, D. Schogl and J.W. Mannhalter Direct PCR of Small Genomic DNA Fragments from Serum A.J. Sandford and P.D. Pare Photoimmunodetection: A Nonradioactive Labeling and Detection Method for DNA O.J. Marvik, M.L. Isaksen, L. Roza and B.H. Lindqvist Quantitation of In Vitro Activity of Synthetic Trans-Acting Ribozymes Using HPLC L. Citti, L. Boldrini, S. Nevischi, L. Mariani and G. Rainaldi Isolation of Full-Length RNA from a Thermophilic Cyanobacterium X.-Z.J. Luo and S.E. Stevens, Jr. BioFeature Applications of Green Fluorescent Protein in Plants S.M. Leffel, S.A. Mabon and C.N. Stewart, Jr. RESEARCH REPORTS Ribonuclease H Renaturation Gel Assay Using a Fluorescent-Labeled Substrate L.-Y. Han, W.-P. Ma and R.J. Crouch Focal Delivery of Neurotrophins into the Central Nervous System Using Fluorescent Latex Microspheres D.R. Riddle, L.C. Katz and D.C. Lo PRODUCT APPLICATION FOCUS Bifunctional lacZa-ccdB Genes for Selective Cloning of PCR Products P. Gabant, P.-L. Dreze, T. Van Reeth, J. Szpirer and C. Szpirer Infrared Fluorescent Detection of D1S80 Alleles from Blood and Body Fluid Collected on IsoCode(TM) Devices R. Roy and L.R. Middendorf NEW PRODUCTS INDEX TO ADVERTISERS From owner-biotechniques@net.bio.net Wed Oct 22 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Richard A. Murphy" Newsgroups: bionet.journals.letters.biotechniques Subject: Hoechst 33342 Date: 23 Oct 1997 05:59:49 -0700 Organization: University of Illinois at Chicago, ADN Computer Center Lines: 9 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <97295.171515U14663@uicvm.uic.edu> NNTP-Posting-Host: net.bio.net Does anyone know if Hoechst 33342 is analogous to fluorescein diacetate in staining bacteria? and: Do different bacteria (gram -ve/gram +ve) take up Hoechst to different degrees? Thanks for your help. Dick Murphy From owner-biotechniques@net.bio.net Wed Oct 22 23:00:00 1997 Path: biosci!biosci!not-for-mail From: ray@leicester.ac.uk (Raymond Dalgleish) Newsgroups: bionet.journals.letters.biotechniques Subject: Re: text book on gene manipulation Date: 23 Oct 1997 14:21:56 -0700 Organization: Department of Genetics, University of Leicester, UK Lines: 17 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <62nhn8$dac_001@gene.le.ac.uk> References: <344E1220.2228@aber.ac.uk> NNTP-Posting-Host: net.bio.net In article <344E1220.2228@aber.ac.uk>, Simon Firek wrote: >I need to teach a 3rd year undegraduate course on the principles of gene >manipulation e.g. cloning, sequencing, PCR etc. > >Could anyone recommend a good up to date text book which would cover >this area. > >Thanks, > >Simon Try "Gene Cloning - an introduction" (3rd edn.) TA Brown (1995) Chapman and Hall Raymond Dalgleish Department of Genetics University of Leicester From owner-biotechniques@net.bio.net Thu Oct 23 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Janis Shackelford Newsgroups: bionet.journals.letters.biotechniques Subject: Re: Prestaining proteins Date: 24 Oct 1997 06:27:56 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <344F9020.1D5E@sunstroke.sdsu.edu> NNTP-Posting-Host: net.bio.net Subject: Prestaining proteins If anyone has responded to my question regarding prestaining proteins, would you please do so again. An incorrect email address previously appeared. My question was: I would like to make my own prestained protein standards for SDS PAGE, similar to those sold by various companies (BioRad, Sigma, etc.) The companies are unwilling to share their techniques. Does anyone have a reference or method? J. Shackelford, San Diego State University jshackel@sunstroke.sdsu.edu From owner-biotechniques@net.bio.net Sat Oct 25 23:00:00 1997 Path: biosci!biosci!not-for-mail From: fenghc Newsgroups: bionet.journals.letters.biotechniques Subject: Look for the p73 protocol Date: 26 Oct 1997 10:26:35 -0800 Organization: biology Lines: 20 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <3453B4F2.3B27@ems.hrbmu.edu.cn> NNTP-Posting-Host: net.bio.net I want to do some work with p73 liked the p53, but there is much little information about it? Who can provide me a protocol of p73? Please send me. fenghc@ems.hrbmu.edu.cn So any detail sent me will be apprecaited. Thank a lot. Feng ************************************************************** Huichen Feng Laboratory of Medical University Harbin Medical University Phone: +86+451+6674798 Habin 150086 Fax: +86+451+6677243 China Email: fenghc@ems.hrbmu.edu.cn ******************************************************************