From owner-biotechniques@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!biosci!not-for-mail From: bbernardy Newsgroups: bionet.journals.letters.biotechniques Subject: Auto Sample Prep Date: 3 Jun 1998 06:04:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Approved: mmccarthy@BioTechniques.com Distribution: world Message-ID: <6l3hl0$ato@net.bio.net> Reply-To: julia@mpsf.com NNTP-Posting-Host: net.bio.net I am wondering if anyone has had any experience with automated sample preparation systems for nucleic acid diagnostics. I would appreciate any advice or experience you may have had with the Tigris system by Gen-Probe, Qiagen's offerings in this area, or other automated systems. Thank you, Julia McKeon Lab Manager MP-Labs From owner-biotechniques@net.bio.net Thu Jun 04 23:00:00 1998 Path: biosci!biosci!not-for-mail From: verma@sol1.lrsm.upenn.edu (Ritu Verma) Newsgroups: bionet.journals.letters.biotechniques Subject: ligation and lambdaphage Date: 5 Jun 1998 13:21:42 -0700 Organization: University of Pennsylvania Lines: 10 Sender: daemon@net.bio.net Approved: mmccarthy@BioTechniques.com Distribution: world Message-ID: <6l9k0m$fbh@net.bio.net> NNTP-Posting-Host: net.bio.net I am trying to ligate the sticky ends of lambda DNA so that I have circular lambda. Does anyone have any experience doing this? I am currently having trouble since I cannot determine whether a regular ligation procedure worked. Thanks Ritu Verma From owner-biotechniques@net.bio.net Tue Jun 23 23:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.journals.letters.biotechniques Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 24 Jun 1998 05:32:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 235 Sender: daemon@net.bio.net Approved: mmccarthy@BioTechniques.com Distribution: world Message-ID: <6mqrk2$lth@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-biotechniques@net.bio.net Thu Jun 25 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Mary McCarthy Newsgroups: bionet.journals.letters.biotechniques Subject: BioTechniques 25(1), July 1998 Date: 26 Jun 1998 07:16:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 118 Sender: daemon@net.bio.net Approved: mmccarthy@BioTechniques.com Distribution: world Message-ID: <6n0agm$91m@net.bio.net> NNTP-Posting-Host: net.bio.net BioTechniques 25(1), July 1998 Table of Contents Benchmarks Preventing Proteolytic Artifacts in the Baculovirus=20 Expression System L.G. Hom and L.E. Volkman Nickel-Induced Oligomerization of Proteins Containing=20 10-Histidine Tags T. Sprules, N. Green, M. Featherstone and K. Gehring Expression Vector Containing an N-Terminal Epitope Tag for Dictyostelium discoideum C.-F. Chen and E.R. Katz Screening of Recombinant DNA Clones by Single-Colony Micro-Lysate/Restriction Enzyme Analysis K.R. Peterson Direct Use of Synthetic Peptides for Antiserum Production N. Sheibani and W.A. Frazier Subtraction with 3-prime Modified Oligonucleotides Eliminates=20 Amplification Artifacts in DNA Libraries Enriched=20 for Microsatellites A. Koblizkova, J. Dolezvel and J. Macas Influence of Magnesium Ion Concentration and PCR=20 Amplification Conditions on Cross-Species PCR J.J. Ely, A. Reeves-Daniel, M.L. Campbell, S. Kohler and W.H. Stone Large- and Small-Scale Preparation of Bacteriophage l Lysate and DNA M.-T. Su, T.V. Venkatesh and R. Bodmer Increased Efficiency of Liposome-Mediated Transfection by=20 Volume Reduction and Centrifugation R.S. Verma, D. Giannola, W. Shlomchik and S.G. Emerson Microscope Slide for Enhanced Analysis of DNA Damage Using=20 the Comet Assay M.J. Smith and K.L. O'Neill The SSCP Phenomenon: Addition of HEPES Buffer Dramatically=20 Affects Electrophoretic Mobility Q. Liu and S.S. Sommer Internet On-Ramp Cyberspace for Biologists Short Technical Reports DNA Compression Caused by an Upstream Point Mutation B.G. Weinshenker, D.D. Hebrink, A.M. Gacy and C.T. McMurray High-Temperature, Nonradioactive Primer Extension Assay for Determination of= a Transcription-Initiation Site M. Yamada, H. Izu, T. Nitta, K. Kurihara and T. Sakurai Genotyping of Human Apolipoprotein E Alleles by the New Qualitative, Microplate-Based CASSI-Detection Assay T. Kohler, A.-K. Rost, K. Purschwitz, S. Vondran, H. Remke,=20 O. Wagner and V. Richter Development of a Yeast Trihybrid Screen Using Stable Yeast=20 Strains and Regulated Protein Expression K.J. Fuller, M.A. Morse, J.H.M. White, S.J. Dowell and M.J. Sims Making Genes Green: Creating Green Fluorescent Protein=20 (GFP) Fusions with Blunt-End PCR Products W. Lo, W. Rodgers and T. Hughes One-Step Fluorescent Probe Product-Enhanced Reverse=20 Transcriptase Assay B.A. Arnold, R.W. Hepler and P.M. Keller Adapting the Biomek=AE 2000 Laboratory Automation=20 Workstation for Printing DNA Microarrays J. Macas, M. Nouzova and D.W. Galbraith BioComputing GeneUp: A Program to Select Short PCR Primer Pairs that=20 Occur in Multiple Members of Sequence Lists G. Pesole, S. Liuni, G. Grillo, P. Belichard, T. Trenkle, J. Welsh and M. McClelland Intensity Modulation of Pseudocolor Images L.E. Hinman and P.J. Sammak Research Reports Direct Quantitation of RNA Transcripts by Competitive Single-Tube=20 RT-PCR and Capillary Electrophoresis N.D. Borson, M.A. Strausbauch, P.J. Wettstein, R.P. Oda, S.L. Johnston= =20 and J.P. Landers Elimination of False Positives Generated Through PCR=20 Re-amplification of Differential Display cDNA G. Miele, L. MacRae, D. McBride, J. Manson and M. Clinton Carbohydrate Affinity PAGE for the Study of Carbohydrate- Binding Proteins P.D. Rye and N.V. Bovin Literature Express New Products Index to Advertisers From owner-biotechniques@net.bio.net Thu Jun 25 23:00:00 1998 Path: biosci!biosci!not-for-mail From: RyangGuk Kim Newsgroups: bionet.journals.letters.biotechniques Subject: [Q] Does promoter of CHO cell work in yeast ? Date: 26 Jun 1998 05:41:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Approved: mmccarthy@BioTechniques.com Distribution: world Message-ID: <6n04ue$r3g@net.bio.net> NNTP-Posting-Host: net.bio.net Hi, I mean as follows. If I took some fragment of chromosome from CHO cell and made it integrated into yeast chromosome, could I have genes from CHO cell DNA expressed in yeast ? In other words, what I wonder about is, whether CHO cell's cis-transcriptional and translational elements (e.g. promoters and ribosome binding sites) are functional in yeast (e.g. Saccharomyces cerevisiae). I could not find any article about it. Thanks in advance.