From owner-biotechniques@net.bio.net Wed Sep 09 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Jan Polman Newsgroups: bionet.journals.letters.biotechniques Subject: DDPCR problem Date: 10 Sep 1998 08:45:02 -0700 Organization: akzo nobel Lines: 18 Sender: daemon@net.bio.net Approved: mmccarthy@BioTechniques.com Distribution: world Message-ID: <6t8s5u$t72@net.bio.net> Reply-To: a.ravestein@organon.oss.akzonobel.nl NNTP-Posting-Host: net.bio.net Hi dear collegues, I've just started on a new project, which core is differential display PCR. The problem which we cann't seem to solve is that the 3' primer scores most of the products in contrast to the 5' primer. I've tried to variate the concentrations of the primers, but this wasn't the key. My last hope is to raise the PCR temperature from 40 degrees Celcius to maybe 45 to 50 degrees Celcius. If you got another solution I'll be much obliged (or howerver you spell this word) My sincere greetings, Frank Cohen f.cohen@organon.oss.akzonobel.nl From owner-biotechniques@net.bio.net Mon Sep 21 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Don Peterson Newsgroups: bionet.journals.letters.biotechniques Subject: Biotech Date: 22 Sep 1998 06:25:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Approved: mmccarthy@BioTechniques.com Distribution: world Message-ID: <6u88g6$s9g@net.bio.net> NNTP-Posting-Host: net.bio.net I am looking for a source of bioluminescent plasmids for transformngE.coli, Traditionally we have used antibiotic resistant plasmids but would like to explore alternatives.. Any suggestions would be greatly appreciated From owner-biotechniques@net.bio.net Mon Sep 21 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Don Peterson Newsgroups: bionet.journals.letters.biotechniques Subject: Biotech Date: 22 Sep 1998 06:25:22 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Approved: mmccarthy@BioTechniques.com Distribution: world Message-ID: <6u88g2$s8a@net.bio.net> NNTP-Posting-Host: net.bio.net I am looking for a source of bioluminescent plasmids for transformngE.coli, Traditionally we have used antibiotic resistant plasmids but would like to explore alternatives.. Any suggestions would be greatly appreciated From owner-biotechniques@net.bio.net Mon Sep 28 23:00:00 1998 Path: biosci!biosci!not-for-mail From: dharmegn@0.0.0.0 (dharmegn) Newsgroups: bionet.journals.letters.biotechniques Subject: Re: Biotech Date: 29 Sep 1998 06:42:30 -0700 Organization: IPB Lines: 5 Sender: daemon@net.bio.net Approved: mmccarthy@BioTechniques.com Distribution: world Message-ID: <6uqo46$n6n@net.bio.net> NNTP-Posting-Host: net.bio.net I think that avoiding the use of antibiotic resistant plasmid will render very difficult the isolation of a single clone (you will probably have a lot of contaminant bacteria