From owner-metabolic-reg@net.bio.net Sun May 05 23:00:00 1996 Path: biosci!ARGOTECH.COM!pvaneikeren From: pvaneikeren@ARGOTECH.COM (Paul van Eikeren) Newsgroups: bionet.metabolic-reg Subject: 1996 GRC Biocatalysis Date: 5 May 1996 17:03:14 -0700 Organization: Argonaut Technologies Inc. Lines: 174 Sender: daemon@net.bio.net Distribution: world Message-ID: <318D4108.2AA7@argotech.com> NNTP-Posting-Host: net.bio.net =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D Gordon Research Conference B I O C A T A L Y S I S Kimbal Union Academy * Meriden, NH, USA * 7-12 July 1996 =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D http://w3.argotech.com/argotech/biocatalysis I am posting this message to inform you of this years=92 Gordon Research = Conference on Biocatalysis. The Conference will be held 7-12 July 1996 = at Kimbal Union Academy in Meriden, New Hampshire, USA. The Conference = brings together an interdisciplinary group of biologists, chemists, and = engineers for a full week of intense discussion and examination of the = frontiers of Biocatalysis. We welcome your application for = participation in year=92s conference. Also, we would be grateful if you = would forward a copy of this message to people that you think might be = interested in attending. The subject of the Biocatalysis Conference is synthetically useful = reactions and processes catalyzed by enzymes or whole cells. We will = address three main themes: =B7 new uses of existing enzymes (e.g., lipases, oxidases, and aldolases); =B7 discovery of new enzymes (e.g., epoxide hydrolases, P-450 = hydroxylases, oxynitrilases, thermophilic organisms); and =B7 structure and protein engineering of enzymes (e.g., new structures of = proteases, transketolase and oxynitrilase, and combinatorial methods = versus site-directed mutagenesis). = We have assembled a notable group of speakers, discussion leaders, and = poster presenters. Attached is a copy of the preliminary program. For = additional information on the Conference including the most recent = update of the program and the poster sessions we suggest that you = connect to our World-Wide Web site at http://w3.argotech.com/argotech/biocatalysis If you do not have access to the World-Wide Web or need additional = information, please contact me by e-mail at the address shown below and = can send you additional information and applications forms. We look forward to receiving your application to the conference. Sincerely, Paul van Eikeren =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D Paul van Eikeren, Co-Chair Vice President, Chemistry Argonaut Technologies Inc. 887 Industrial Road, Suite G San Carlos, CA 94070 USA Voice: +1 415 598 1350 ext. 217 Fax: +1 415 598 1359 pvaneikeren@argotech.com 74260.1024@compuserve.com =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D P R O G R A M I N F O R M A T I O N (Updated on 25 March 1996) =3D=3D Opening Session: * Stanley Roberts (Liverpool, UK) Enzymic Baeyer Villiger Reaction * J. John Holbrook (U. Bristol, UK) Getting the Products Off Enzymes = Used in Bulk Chemoenzymic Synthesis =3D=3D Structure and Engineering of Hydrolases for Organic Synthesis * Sabine L. Flitsch (U. Edinburgh, UK) Design and Synthesis of = Enzyme-Cleavable Linkers for Solid Phase Synthesis * Guy G. Dodson (York U., UK) Nucleophilic Attack at the Carbonyl in = Hydrolases: The Varying Stereochemistry at the Nucleophile * Franz Effenberger (U. Stuttgart, Germany) New Results on Preparation = and Application of Chiral Cyanohydrins * Robert Menard (NRC Biotechnology Research Institute, Canada) Protein = Engineering of Cysteine Proteases: From a Better Understanding of Their = Function to Redesigning the Catalytic Activity =3D=3D Molecular Evolution of Subtilisin * Frances Arnold (California Institute of Technology, USA) Directed = Evolution of p-Nitrobenzyl Esterase * Marcus Ballinger (Genentech, USA) Subtilisin BPN Variants Designed for = Cleavage of Multibasic Substrates Multibasic Substrates = * Volker Schellenberger (Genencor, USA) Directed evolution of a = Bacillus protease =3D=3D New Application of Hydrolases * Herbert Waldmann (Karlsruhe, Germany) Bioorganic Synthesis and = Biological Signal Transduction * Milton Zmijewski (Lily, Indianapolis, USA) Enzymatic Removal of = Protecting Groups in the Synthesis of Pharmaceuticals * Kazuo Achiwa (Shizuoka U.) Lipase-Catalyzes Asymmetric Synthesis of = Optically-Active Medicines: New Strategies and their Application =3D=3D Discovery versus Engineering of New Enzymes * John Arnett (Recombinant Biocatalysis, USA) Enzymes from Thermophilic = Microorganisms * Gunter Schneider (Karolinska Institute, Stockholm, Sweden) Toward = Tailoring Enzymes for Asymmetric Synthesis: Protein Engineering of = Transketolase =3D=3D Aldolases and Glycosyl Transfer * Eric Toone (Duke University) Pyruvate Aldolases * Vladimir Kren (Czech Academy of Sciences) Enzymatic Glycosylation of = Pharmacologically Active Compounds: Multienzymatic Approaches and New = Enzymes =3D=3D New Hydrolases * Roland Furstoss (U. Aix-Marseille, France) Enantioselective = Biotransformations with Epoxide Hydrolases * Kenji Soda (Kyoto, Japan) 2-Haloacid Dehalogenases: Their Functions, = Structures, and Applications =3D=3D Oxidations * Aleksey Zaks (Schering-Plough, Union, NJ, USA) Chloroperoxidase * Bernhard Hauer (BASF AG, Ludwigshafen, Germany) Selection of = Biocatalysts for the Preparation of Chiral/Chemical Building Blocks * Roger Sheldon (Delft U. Netherlands) Enantioselective Aminolysis and = Selective Oxidations Mediated by Chloroperoxidase =3D=3D Large Scale Industrial Applications * Kevin DiGregorio (Union Carbide, USA) Polyester Hydrolysis * Robert Dicosimo (Dupont, Wilmington, DE, USA) Scale-Up of a = Biocatalytic Route to N-Phosphonomethylglycine (Glyphosate) Using Whole = Cell Transformants From owner-metabolic-reg@net.bio.net Sun May 05 23:00:00 1996 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!in2.uu.net!nntp.earthlink.net!usenet From: Adam Goldfine Newsgroups: bionet.metabolic-reg Subject: Free Audio Cassette Date: Mon, 06 May 1996 00:30:44 +0000 Organization: GeoServe Communications Lines: 42 Message-ID: <318D4834.1A8C@geocorp.com> Reply-To: goldfine@geocorp.com NNTP-Posting-Host: pool001.maxf.los_angeles.ca.us.dynip.earthlink.net Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 2.01 (Macintosh; I; PPC) No Strings! No Obligation! The most powerful US Government approved antioxidant (antioxidants combat aging) is now available at : http://geocorp.com/pycnogenol/ Proven results for a variety of symptoms. Order the audio cassette, free of charge, and learn about this amazing antioxidant, Pycnogenol. Visit the web site for more information on this product. Excerpt: The extraordinary antioxidant properties of Pycnogenol ® are attributed to the fact that it is not a single bioflavonoid but a complex combination of bioflavonoids. Pycnogenol ® has an exceptionally high level of proanthocyanidins (the main active ingredient), and it also contains other water soluble bioflavonoids. This unique combination provides a powerful, completely balanced all-natural antioxidant. According to recent studies, a growing number of experts agree that we need additional antioxidants to support our natural ability to fight free radicals. A free radical is an oxygen molecule with an odd number of electrons. Because electrons are normally paired, this creates an imbalance, resulting in unstable, reactive and destructive molecules. While some free radical activity is necessary for the proper functioning of the body, too much may cause damage. Unfortunately, many of today's environmental influences bombard us with free radicals and possibly suppress our natural ability to hold them in check. Fortunately, research has identified substances known as bioflavonoids (especially proanthocyanidins) as an excellent source to help fight free radicals. Although some bioflavonoids occur naturally in fruits and vegetable, only 20% of Americans eat the recommended daily serving of these foods. The lack of dietary intake is compounded by the fact that growing methods and processing procedures actually deplete and even destroy these naturally occurring antioxidants. Pycnogenol® is an excellent source of antioxidant supplementation. It is water soluble and exceptionally high in proanthocyanidins and other bioflavonoids. These unique qualities provide a superior natural antioxidant that is easily absorbed. From owner-metabolic-reg@net.bio.net Tue May 07 23:00:00 1996 Newsgroups: bionet.software,bionet.metabolic-reg,bionet.general,bionet.cellbiol,sci.bio.misc From: SINCLAIRB@Agresearch.cri.nz (BRUCE SINCLAIR) Subject: Re: I am looking for NAD(P)H:FMN oxidoreductase (EC 1.6.8.1) Organization: AgResearch, Grasslands References: <4m639s$g8t@oac2.hsc.uth.tmc.edu> X-Newsreader: News Xpress Version 1.0 Beta #4 Date: Wed, 08 May 96 04:08:37 GMT NNTP-Posting-Host: 202.20.104.151 Message-ID: <31902773.0@news.palm.cri.nz> Lines: 13 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!brighton.openmarket.com!decwrl!waikato!news.massey.ac.nz!news.palm.cri.nz!gra41 Xref: biosci bionet.software:15435 bionet.metabolic-reg:733 bionet.general:21532 bionet.cellbiol:4632 sci.bio.misc:3149 In article <4m639s$g8t@oac2.hsc.uth.tmc.edu>, "Dr. Ashish K. Gupta" wrote: NNTP-Posting-Host: net.bio.net Dear colleagues, This is a follow-up on the next conference of the Society for Physical Regulation in Biology and Medicine (SPRBM) in Chicago, Oct. 9-12, 1996. The Program Committee Chair (Prof. Subrata Saha) has asked me to say that the ABSTRACT DUE DATE IS 31 MAY, not 10 May as was stated earlier. This gives us all extra time to get in a contributed abstract. If any of you are working in areas that relate to the control of biological systems by physical forces (mechanical, electromagnetic, ultrasound, thermal...), including cell mechanics, bone remodeling, growth and repair stimulation, etc., this is where you have an opportunity to share your new findings and/or get useful feedback and hot ideas for tomorrows grant proposals. ABSTRACT FORMAT and SUBMISSION: (by 31 MAY) Use high quality, crisp type to allow photo-reproduction. Title, bold and centered; below that, authors and affiliations, centered. 500 word, single spaced abstract (right and left justified if possible) with 1 inch margins on 8.5 x 11.0 inch paper. One (1) page; a second page can be used to include figures and tables. The abstract should be substantive and give results of original work done. SEND TO: Soc. for Physical Regulation in Biology and Medicine 9650 Rockville Pike Bethesda, Maryland 20814, U.S.A. For updated program information as it becomes available please contact: tel: 301-571-0680 Fax: 301-530-7049 E-mail: sprbm@faseb.org Remember also the 3 special instructive symposia with leading experts: 1. Mechanical factors on VERTEBRATE EVOLUTION (dinosaurs, etc.). 2. OSTEOARTHRITIS and physical forces. 3. MECHANICAL SIGNAL transduction in cells. Joe Spadaro Joseph A. Spadaro, Ph.D. Associate Professor - Orthopedic Research S.U.N.Y. Health Science Center - Syracuse spadaroj@vax.cs.hscsyr.edu From owner-metabolic-reg@net.bio.net Thu May 09 23:00:00 1996 Path: biosci!ns1.faseb.org!lamarck.sura.net!newsfeed.internetmci.com!swrinde!tank.news.pipex.net!pipex!lade.news.pipex.net!pipex!tube.news.pipex.net!pipex!hole.news.pipex.net!pipex!oleane!jussieu.fr!infobiogen.fr!pasteur.fr!univ-lyon1.fr!ws41.cnusc.fr!ciril.fr!usenet From: fiervill@clsh.u-nancy.fr (Thierry Fierville) Newsgroups: bionet.metabolic-reg Subject: Instruction for Authors Metabolism Date: 10 May 1996 21:01:19 GMT Organization: Laboratoire de Psychologie - Université Nancy 2 Lines: 5 Distribution: world Message-ID: <4n0aqv$hf1@arcturus.ciril.fr> NNTP-Posting-Host: pppcri1.plg.u-nancy.fr Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: News for Windows NT X1.0-89 Can anybody forward me the Instructions for Authors of the journal Metabolism to the following e-mail adress: karin@cmp.u-nancy.fr ?! Thank you very much ! From owner-metabolic-reg@net.bio.net Fri May 10 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!viking.ucsalf.ac.uk!news.salford.ac.uk!aber!not-for-mail From: dbk@aber.ac.uk (Douglas B. Kell) Newsgroups: bionet.metabolic-reg Subject: Gordon Research Conference on Macromolecular Organization and Cell Function Date: 10 May 1996 10:06:06 GMT Organization: University of Wales, Aberystwyth Lines: 212 Message-ID: <4mv4ee$9lf@infoserv.aber.ac.uk> NNTP-Posting-Host: pcjaeh.dbs.aber.ac.uk Mime-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII X-Disclaimer: Warning - Sender not verified X-Newsreader: WinVN 0.99.6 This is the conference for folk who want to know what the inside of a cell is REALLY like - not the mush that appears in a blendor ..... The Fixed Fee (Registration) for the Oxford Conference will be $675, which includes (covers) lodging and meals for the 5 day conference. This conference will have two poster sessions - these are always very well received, and provide a good opportunity to present your work, as well as interact with others attending the conference. Hope to see you in Oxford next September. ============================================================================== Gordon Research Conferene MACROMOLECULAR ORGANIZATION AND CELL FUNCTION Queen's College, Oxford, 1-6 September 1996 Chair: James Clegg (U. California, Davis) Vice-Chairs: Douglas Kell (U. Wales) and John Wilson (Mich. State U.) (Discussion Leaders for the sessions are indicated by asterisks; topics, not titles, are given.) DAY 1. MONDAY AM - Introduction to the Conference: *James S. Clegg CELL STRUCTURE Nuclear Organization: Jeffery Nickerson (MIT) Cytoplasmic Organization: Ken Giuliano (U. Pittsburgh Sch. Med.) Prokaryotic Cells: Frank Mayer (U. Goettingen) PM - INTRACELLULAR ENVIRONMENT Water in Cells and Macromolecules: *Philippa Wiggins (U. Auckland Sch. Med.) Crowding in the Aqueous Phase: Allen Minton (NIH) Cell Volume & Metabolism: Florian Lang (Tuebingen) DAY 2. TUESDAY AM - MINIMALLY INVASIVE STUDIES Nuclear Magnetic Resonance: *Chris Hardin (U. Missouri Sch. Med.) Fluorescence: Ron Lynch (U. Arizona Sch. Med.) Enzyme Cytochemistry: C. VanNoorden (U. Amsterdam Med. Cent.) New Approaches: G. Albrecht-Buehler (Northwestern U.) PM - THEORY & MODELING OF METABOLISM Overview and Importance: *G. Rickey Welch (U. New Orleans) Control Theory: Pedro Mendes (U. Wales) Applications: Hans Westerhoff (Free U. Amsterdam) DAY 3. WEDNESDAY AM - COMPARTMENTATION, COMPLEXES, CHANNELING-I Phosphocreatine Circuit Microsymposium: *John Wilson (Mich.State U.) Theo Wallimann (Swiss Fed. Inst. Tech.) Klaas Nicolay (U. Utrecht) Krebs Cycle Organization: Paul Srere (Dallas VA Med. Center) PM - COMPARTMENTATION, COMPLEXES, CHANNELING-II Intact Cell Biochemistry: *Michael Berry (Flinders U. Sch. Med.) Fatty Acid Oxidation: Horst Schulz (City College, CUNY) Glycolytic Pathway: Judit Ovadi (Hungarian Acad. Science) DAY 4. THURSDAY AM - CYTOSKELETON & MACROMOLECULAR FUNCTION Enzyme/Cytomatrix Interactions: *Harvey Knull (U. North Dakota Sch. Med.) mRNA Translocation & Mts: Kathy Suprenant (U. Kansas) MF/Enzyme Associations: Len Pagliaro (U. Washington) Signalling: Kermit Carraway (U. Miami Sch. Med.) PM - THE PROTEIN SYNTHESIZING SYSTEM Ribosome Ultrastructure: Joachim Frank (SUNY Albany) Channeling: *Murray Deutscher (U. Miami Sch.Med.) Cytomatrix Associations: John Hesketh (Rowett Res. Inst., Aberdeen) DAY 5. FRIDAY AM - NOVEL PROTEIN-PROTEIN INTERACTIONS Molecular Chaperones: *William Welch (UC San Francisco) Proteasomes: Mark Hochstrasser (U. Chicago) Two-Hybrid System: Stanley Fields (U. Washington) The AAA Module: Michel Duget (CNRS, U. Paris Sud) ----------------------------------------------------------------------------- Applications can be made electronically using this form: ****Please e-mail this application to: grc@grcmail.grc.uri.edu Applications are required of all individuals participating in a Conference. This applies to the Chair, Co-Chairs, Vice Chair, Discussion Leaders, Speakers, Poster Presenters, and Conferees. DEADLINE FOR RECEIPT OF APPLICATIONS IS 6 WEEKS PRIOR TO THE CONFERENCE ----------------------------------------------------------------------- CONFERENCE LOCATION: Oxford, UK CONFERENCE NAME: Macromolecular Organization and Cell Function CONFERENE DATE: 1-6 September 1996 NAME: ORGANIZATION: BUSINESS ADDRESS: CITY: STATE: COUNTRY: ZIP CODE: PHONE: FAX: E-MAIL: ----------------------------------------------------------------------- ACCOMODATIONS FOR APPLICANT: GUEST(S): CHILDREN: ----------------------------------------------------------------------- I work in ACADEMIC INSTITUTION:___ If predominantly undergraduate, please check here:___ GOVERNMENT AGENCY:___ INDUSTRIAL CORPORATION:___ ----------------------------------------------------------------------- Please check: CHAIR:___ VICE CHAIR:___ DISCUSSION LEADER:___ SPEAKER:___ POSTER PRESENTER:___ ATTENDEE:___ ----------------------------------------------------------------------- Previous conferences attended NONE:___ 1-5:___ 6-10:___ 10+:___ ----------------------------------------------------------------------- Your Position? GRADUATE STUDENT:___ POSTDOC:___ RESEARCH SCIENTIST:___ PROFESSOR:___ RESEARCH DIRECTOR:___ PROGRAM MANAGER:___ OTHER:___ ----------------------------------------------------------------------- Are you personally involved in research activities in the subject area of the conference? (Y/N):___ How many papers have you published during the past three years in the subject area of the conference?:___ ----------------------------------------------------------------------- You are invited to submit an abstract for a poster presentation at the Conference. Many Chairs find abstracts very useful in making decisions concerning admissions to their Conferences. Applications are referred to the Conference Chair in accordance with the established regulations. Following the Chair's acceptance, the registration card will be sent to you. Please complete it and return it immediately with payment of the FIXED FEE. ----------------------------------------------------------------------- GORDON RESEARCH CONFERENCES ADMITS SCIENTIFICALLY-QUALIFIED CONFEREES OF ANY SEX, RACE, RELIGION,AGE, COLOR, AND NATIONAL ORIGIN. ----------------------------------------------------------------------- The recording of lectures by tapes, etc. and the photography of slide material are prohibited. Printed reference to Gordon Research Conference papers and discussion is not permittted. Authors are requested to omit references to the Conferences in any publication. Guests are not permitted to attend the conference lectures and discussion sessions. Each member of the Conference agrees to these regulations when registration is accepted. ---------------------------------------------------------------------------- From owner-metabolic-reg@net.bio.net Sat May 18 23:00:00 1996 Path: biosci!student.dtu.dk!okjans From: okjans@student.dtu.dk (Jan Schripsema) Newsgroups: bionet.metabolic-reg Subject: (none) Date: 19 May 1996 01:29:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 104 Sender: daemon@net.bio.net Distribution: world Message-ID: <199605190826.KAA09208@aix0.kbar.dtu.dk> NNTP-Posting-Host: net.bio.net --=====================_832526739==_ Content-Type: text/plain; charset="us-ascii" I would like to become a member of the mca discussion group. Will it be sufficient to send this message or should I contact someone in special? Further text will be attached to this message. 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--=====================_832526739==_ Content-Type: text/plain; charset="us-ascii" ************************************* Jan Schripsema The Technical University of Denmark Dept. of Organic Chemistry Building 201 DK-2800 Lyngby Denmark tel: **-45-45252111 fax: **-45-45933968 ************************************* --=====================_832526739==_-- From owner-metabolic-reg@net.bio.net Mon May 20 23:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!uvsq.fr!Newsmaster From: Faroux-Kerboul Newsgroups: bionet.metabolic-reg Subject: Halobacterium marismortui Date: Tue, 21 May 1996 09:43:05 -0700 Organization: Universite de Versailles/St Quentin en Yvelines - France Lines: 2 Message-ID: <31A1F299.3E73@genome.uvsq.fr> NNTP-Posting-Host: pclic8.genetique.uvsq.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) Biology student, I'm looking for informations on the metabolism of bacteria living in hypersaline environment ( Dead Sea ). Thanks! From owner-metabolic-reg@net.bio.net Mon May 20 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Simon Eaton Newsgroups: bionet.metabolic-reg Subject: 3rd Conference on fatty acid oxidation and ketogenesis Date: 21 May 1996 11:25:05 -0700 Organization: Newcastle University, UK Lines: 58 Sender: daemon@net.bio.net Distribution: world Message-ID: <4nphja$3s8@whitbeck.ncl.ac.uk> NNTP-Posting-Host: net.bio.net 3rd Fatty Acid Oxidation & Ketogenesis Conference Saturday 7th to Monday 9th September 1996, Institute of Child Health, London The Third International Conference on Fatty Acid Oxidation and Ketogenesis will be held in London this year, at the Institute of Child Health and Great Ormond Street Hospital for Children NHS Trust, University of London, from Saturday 7th to Monday 9th September 1996, immediately after the Biochemical Society Meeting at Queen Mary and Westfield College, University of London (4-6 September). It is being held over the weekend in order to allow delegates to purchase cheap flights. The Conference Chairman and Organizer is Dr Patti Quant, and the Meeting Secretary is Dr Simon Eaton. This year it will be necessary for us to charge a registration fee of £100 for senior participants and £66 for postgraduate students and postdoctoral workers under the age of 27 years. The fee will cover the cost of all refreshments and meals during the entire Meeting (including the Conference Dinner but excluding breakfasts), hiring the rooms, equipment and catering staff, publicity and notebooks, tourist information and maps, badges etc. but will not cover accommodation. As before, we shall be happy to send information on travel, hotels etc. and organize and book accommodation of the kind required if you would like us to do so. The sessions will be: 1.Carnitine Palmitoyl Transferase I, II etc. & the carnitine carrier 2. Mitochondrial HMG-CoA synthase etc. & ketogenesis 3. Mitochondrial beta-oxidation & the electron transfer chain 4. Peroxisomal beta-oxidation If you and/or members of your group would like to attend/participate, please contact Dr Patti Quant as soon as possible in order to allow us to prepare a timetable, list of speakers etc. Dr Patti A. Quant Unit of Paediatric Surgery Institute of Child Health 30 Guilford Street LONDON WC1N 1EH Phone: +44 171 242 9789 ext. 2136 Fax: + 44 171 404 6181 E-Mail: P.Quant@ich.ucl.ac.uk Details of invited participants etc. are available at http://www.ncl.ac.uk/~nchwww/faoxk.html Simon Eaton Meeting Secretary From owner-metabolic-reg@net.bio.net Tue May 21 23:00:00 1996 Path: biosci!student.dtu.dk!okjans From: okjans@student.dtu.dk (Jan Schripsema) Newsgroups: bionet.metabolic-reg Subject: (none) Date: 22 May 1996 08:57:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 73 Sender: daemon@net.bio.net Distribution: world Message-ID: <199605221554.RAA23224@aix0.kbar.dtu.dk> NNTP-Posting-Host: net.bio.net I have recently started to read about metabolic control analysis. I was very happy to know that there is a theoretical treatment of the effects of enzyme amounts on "in vivo" pathway fluxes. I work in the area of phytochemistry, more precisely with the regulation of terpenoid indole alkaloid accumulation in plant cell suspension cultures. I do not know any articles concerning plant secondary metabolites which use m.c.a., do you? I have read the original article by H. Kacser and J.A. Burns and am gaining further insight reading more recent papers. Most of them concern theoretical simulations and animal or microorganism biochemistry. The papers on plant biochemistry that I have been able to find deal with primary metabolism (starch and sugar accumulation). I am trying to apply mca to my own experiments but am having some difficulties. My question is the following: Which enzyme(s) of the biosynthetic pathway limit the biosynthesis of the product of my interest or which enzyme(s) have the highest flux control coefficient? Before knowing of the existence of mca I made the following experiment to try to answer part of this question: I compared the alkaloid accumulation and the activities of enzymes of its biosynthetic pathway of cells growing under normal culture conditions with cells to which a substrate of the pathway had been added. This was done for two cell lines differing in their alkaloid accumulation capacity. In a previous experiment I had found that the differences in the alkaloid accumulation of the two cell lines was due to differences in biosynthetic rates and not breakdown rates. The levels of several of the enzymes involved in the biosynthesis were also different, though often not proportional or even related to levels of biosynthesis. When loganin (an intermediate of the terpenoid indole alkaloid biosynthetic pathway) was added to the cultures, alkaloid biosynthesis was increased in both cell lines (from 5 to 100 times). The levels of all the biosynthetic enzymes investigated remained the same. From these results I concluded that the amounts of the enzymes I measured did not limit terpenoid indole alkaloid accumulation under normal culture conditions, since the same levels could give rise to higher fluxes when precursor was added. In mca terminology: their flux control coefficient is low (but I can not really calculate them can I?). Another conclusion was that enzymes with higher flux control coefficients will be found proximal (before) the intermediate loganin. Since I do not work directly with gene technology and if I would start to, I still know it is quite laborious, I am trying to find a way to avoid it but still use mca. Is this possible? Is it possible to consider an added intermediate of the pathway under study a parameter and calculate its response coefficient and so locate the enzymes which will have higher flux control coefficients? I have not yet found any similar experiment using mca. Thank you in advance for all comments and suggestions. Denise Dagnino ************************************* Denise Dagnino The Technical University of Denmark Dept. of Organic Chemistry Building 201 DK-2800 Lyngby Denmark tel: **-45-45252111 fax: **-45-45933968 ************************************* From owner-metabolic-reg@net.bio.net Wed May 22 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: mdb1@mole.bio.cam.ac.uk (Martin Brand) Newsgroups: bionet.metabolic-reg Subject: reply to Denise Dagnino Date: 23 May 1996 12:48:07 +0100 Lines: 43 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4o1j9n$8hl@mserv1.dl.ac.uk> Original-To: btk-mca@dl.ac.uk Denise You can't really conclude that steps in a pathway beyond a certain metabolite have no flux control just because adding the metabolite increases the flux, so you can't conclude that steps before the metabolite must have most of the control: it depends on the relative elasticities of the groups of reactions before and after your metabolite to the concentration of that metabolite. If you choose to use gene expression to measure control coefficients, you need to be aware that the numbers you get refer to the effect of the gene manipulation on the flux with a long relaxation time, and this is not neccessarily the same as the effect of the enzyme on the flux with a short relaxation time. So if the gene expression causes secondary unidentified changes in the system (such as consequential changes in the expression of other genes) these changes appear with gene manipulation but not with direct inhibition of the enzyme or from elasticities. Which type of flux control coefficient you want to know is a matter of choice, but they may differ. To avoid gene manipulation you can either inhibit your pet enzyme and see what happens, or do the analysis by measuring elasticities and solving the relevant equations. I think you can't make a metabolite a parameter without altering the nature of the pathway under investigation: it is now (at least) two pathways, one leading to your clamped metabolite and the other leading away from it. But you can solve the control around your metabolite by measuring the relative changes in the fluxes in these pathways when you raise the metabolite slightly, since the flux changes are related to the elasticity ratios and the control coefficients. Martin Brand Martin D. Brand Department of Biochemistry, University of Cambridge Tennis Court Road, Cambridge CB2 1QW UK phone: +44 1223 333648, fax: +44 1223 333345 Internet: http://www.bio.cam.ac.uk/dept/biochem/brand/ From owner-metabolic-reg@net.bio.net Fri May 24 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!news.ac.net!news.bconnex.net!news2.insinc.net!pegasus.odyssee.net!news From: Carolyn Petit-Turcotte Newsgroups: bionet.metabolic-reg Subject: Chromogranins Date: 25 May 1996 01:20:10 GMT Organization: Odyssee Internet Lines: 12 Message-ID: <4o5n8a$qbj@pegasus.odyssee.net> NNTP-Posting-Host: pool14_11.odyssee.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:bionet.metabolic-reg Hello! I am looking for antibodies against chromogranins... If you have one or know where I can get some, please e-mail to: carrie@odyssee.net Thanks! From owner-metabolic-reg@net.bio.net Thu May 30 23:00:00 1996 Path: biosci!ciit.org!schlosser From: schlosser@ciit.org (Paul M. Schlosser) Newsgroups: bionet.metabolic-reg Subject: Re: enzymes Date: 31 May 1996 13:20:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net >Hello! > >Are there any references out there for multifunctional enzymes? > >Thanks! Please email to: pollack.1@osu.edu Cytochrome P450s are typically multi-functional (act on multiple substrates)? Is that the sort of thing you are looking for? Paul S. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Paul M. Schlosser, Ph.D. Chemical Industry Institute of Toxicology (CIIT) P.O. Box 12137 6 Davis Drive (not needed for regular mail) Research Triangle Park, NC 27709 Ph:(919) 558-1243; FAX:(919) 558-1300; schlosser@ciit.org _________________________ _____________________________________ _________________________ | | _________________________ | ////// //// //// ////////// | _________________________ | // // // // // // // | _________________________ | // // // // | _________________________ | // // // // | _________________________ | // // // // // | _________________________ | ////// //// //// //// | _________________________ |_____________________________________| From owner-metabolic-reg@net.bio.net Thu May 30 23:00:00 1996 Path: biosci!rutgers!csn!nntp-xfer-1.csn.net!magnus.acs.ohio-state.edu!medmicro1.med.ohio-state.edu!pollack.1 From: pollack.1@osu.edu (J. Dennis Pollack) Newsgroups: bionet.metabolic-reg Subject: enzymes Date: Fri, 31 May 1996 19:01:53 GMT Organization: The Ohio State University Lines: 5 Message-ID: NNTP-Posting-Host: medmicro1.med.ohio-state.edu Keywords: multifunctional enzymes X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #4] Hello! Are there any references out there for multifunctional enzymes? Thanks! Please email to: pollack.1@osu.edu